Method for pathomorphological determination of prescription of myocardial infarction
SUBSTANCE: pathomorphological determination of the prescription of myocardial infarction is ensured by fixing a tissue sample and placing it into paraffin. Sections are prepared, de-waxed, heated, washed in a buffer solution, incubated in a moisture chamber and processed with a developing agent, dehydrated and enclosed by a medium. The reagent is presented by matrix metalloprotease 9 antibodies in dilution 1:100-1:250. The sections are incubated with the reagent at a temperature of 25°C and a relative humidity of 100% for 60 minutes. If the microscopy detects the bright-coloured neutrophils in peri-infarction vessels and within the infarction zone, the prescription is stated to be 2 hours to 1 day. If observing the neutrophil degranulation and the bright colour of an extracellular matrix within the infarction, the prescription is 1 to 2 days. If the coloured fibroblasts are found in the infarction border, the prescription is stated to be within 3 to 30 days.
EFFECT: method enables differentiating the prescription of myocardial infarction within 2 hours to 30 days.
1 ex, 3 dwg
The present invention relates to medicine, namely to pathological anatomy.
It is known that the exact determination of the period of limitation of myocardial infarction is of great importance for the pathologist.
The known method of macroscopic diagnosis of early stages of myocardial infarction based on the interaction of tellurite potassium dehydrogenase tissues. Outside of myocardial telluric potassium colors myocardium in grey or black color, and the area of necrosis remains unpainted, due to the destruction of these enzymes in the zone of myocardial infarction (Fingers M.A., Anichkov NM Pathological anatomy. 2 so, Vol.2, Part 1. - M.: Medicine, 2001. - S).
The disadvantage of this method is the lack of differential timing of the onset of myocardial infarction. There is a method allows to detect only necrotic stage of myocardial infarction (the time from 2 hours up to 3 days), because after the onset of the stage of organization of the activity of dehydrogenases in the tissue is gradually restored.
In addition, the use of this method makes it difficult to obtain high-quality samples for histological and immunohistological studies and reduces the quality of the documentation of the morphological changes in the myocardium due to the fact that the dyes steadfastly dyed fabric in grey color. When [daln] Isham mandatory study material this coloration prevents staining of standard dyes.
The closest in technical essence to the present invention is a method for the diagnosis of ischemic necrosis of the heart muscle (U.S. Pat. 2094806 Russian Federation, IPC G01N 33/68, G01N 33/50, a method for the diagnosis of necrosis in histological sections / Kozlov D.V.; applicant and patentee, Novokuznetsk state Institute of advanced training of physicians, No. 94005367/14; Appl. 14.02.1994; publ. 27.10.1997).
The known method is as follows. Fragments of the cardiac muscle is fixed with 15% aqueous neutral formalin solution for days at room temperature. Subsequently the material is subjected to paraffin filling, then prepare histological sections, which deparaffinized processing in three portions of o-xylene and subsequent heating in a thermostat at 37°C for 3 hours. Dehydration slices reach the 3 minute treatment in each of the four portions of ethanol (absolute, 96% 70% and 56%). Next, the slices for 5 minutes, transferred to 0.05 M buffered saline with a pH of 7.6. Blocking of endogenous peroxidase carry out a fresh solution of 3% hydrogen peroxide for 10 minutes, washed with buffered saline for 5 minutes. Further cuts stand under the drop of a 5%solution of horse serum albumin. After that, the excess albumin shake and gently wipe the glass around the slices. the and last put a drop afinno-purified rabbit primary antibodies against associated with pregnancy Alphas-glycoprotein at a concentration of 8 µg/ml Incubation of slices is carried out for 30 minutes at 37°C in a humid chamber, and then washed twice buffered saline solution. Incubated histological sections in the secondary biotinylated antibodies in a dilution of 10 µg in 1 ml of buffered saline solution for 30 minutes, then washed buffered saline for 5 minutes. Then treated slices complex avidin-peroxidase for 30 minutes and washed buffered saline solution. Immunological manifestation exercise 0.03% solution of the substrate on the basis of diaminobenzidine (DAB Chromogen) for 10 minutes the Reaction is stopped by rinsing the sections in two portions of distilled water. Cell nuclei Domracheva with hematoxylin for 3 minutes Then cuts to carry through the fresh spirits ascending concentrations (56% 70% 96%absolute ethanol) and three servings of o-xylene, and then sign in balsam.
In the study of prepared microslides mark a clear staining foci of ischemic necrosis of the heart muscle in the area of myocardial infarction. The focus of necrosis has a brown color, and other fabric - yellow-gray color.
The disadvantage of this method, as similar, is the lack of a precise definition of the period of limitation of myocardial infarction, because the prototype method call which allows to identify only necrotic stage.
The disadvantages of this method include that the half-life of complexes associated with pregnancy A2-glycoprotein with proteinase does not exceed 2 minutes. Therefore, in half an hour, virtually all complexes of enzymes associated with pregnancy A2-glycoprotein reach target organs, particularly the liver, where and are metabolized. Outside of the blood vessels this distribution has different characteristics, as high molecular weight complexes prevents them from entering the blood vessels (Zorin N.A. Associated with pregnancy-ALPHA-2-GLYCOPROTEIN. NEWS "Vector-best", N 9, September, 1998. CIT. The link http://www.vector-best.ru/nvb/st9_5.htm 29.04.12 year). Therefore, identifying complexes in the border zone of myocardial infarction is limited to a very short period; identification of the complexes is only possible in the zone of necrosis, i.e. outside the zones intact blood supply.
The task of the invention is to develop a method for determining the period of limitation of myocardial infarction with pathomorphological studies.
The technical result of the proposed method is the determination of the period of limitation of myocardial infarction from 2 hours to 30 days.
The technical result is achieved in that the method pathological determining the limitation of the occurrence of a heart attack is ICARDA includes the fixation of the tissue sample, place it on the wax, making slices, dewaxing and warming up, laundering in the buffer solution, incubation in a humid chamber with the reagent and processing developing agent, dehydration and conclude on Wednesday.
The main distinctive technique proposed method is that as the reagent is used antibodies to the matrix metalloprotease 9 in a dilution of 1:100-1:250.
Distinctive reception of the proposed method is that the incubation with the reagent is carried out at a temperature of +25°C and a relative humidity of 100%. Incubation duration is 60 minutes.
Differences between the proposed method lies in the fact that microscopic detection in drug brightly colored neutrophils in the blood vessels periinfarct zone and brightly colored neutrophils in the zone of infarction establish a period of limitation of infarct from 2 hours to 1 day, in identifying the degranulation of neutrophils and bright colors of the extracellular matrix in the zone of the infarct - from 1 to 2 days, and when the detection of stained cells fibroblast number in the border zone of the infarct - from 3 to 30 days.
Comparative analysis of the proposed technical solutions to the prototype allows to make a conclusion on the conformity of the proposed technical solution the criteria of the invention of "novelty."
The inventive method provides achievement is the perceived by the applicant of the technical result, namely the ability to determine when pathomorphological studies of the period of limitation of myocardial infarction from 2 hours to 30 days while reducing the complexity and duration of the study.
Thus, the authors of the proposed method is established that the used primary antibodies bind with substances synthesized by neutrophils, fibroblastoma, as well as substances in the extracellular matrix in a strictly defined time intervals after myocardial infarction. Therefore, the use of immunohistochemical staining of histological preparations, as the primary antibody to the matrix metalloprotease 9, allowed the authors of the proposed method to differentiate the expiration of the onset of myocardial infarction with pathomorphological study.
The advantage of the proposed method is that it allows you to identify not only the necrotic stage of myocardial infarction, but also the stage of the organization that determines its strength in comparison with the prototype.
The above can conclude that the technical solutions according to the criterion of "inventive step".
The method constituting the invention, intended for use in medicine. The possibility of its fulfillment is confirmed as described in the application techniques and the medium is you. This gives grounds to believe that the proposed solution meets the criteria of the invention "industrial applicability".
The essence of the proposed method is illustrated by a specific example. The following example serves to illustrate, but not limit the invention.
The object of the study was postmortem material 30 patients who died from acute myocardial infarction. In all cases diagnosed with primary acute transmural myocardial infarction. The average age was 63.7 years. Male 18, female 12. The timing of the onset of heart attack patients were distributed as follows: 16 patients died in the period up to 3 days; 14 patients - in time from 3 days to 1 month.
Tissue samples from the zone of myocardial infarction and edge to her area recorded a 10% solution of neutral formalin and embedded in paraffin blocks according to the standard technique (Merkulov, G. A. Course histopathological techniques. - L.: Medicine, 1969. - S-15, 52-59).
Then cooked slices with a thickness of 5 μm, which were mounted on glass coated with poly-L-lysine. Was deparaffinization drugs beginning in toluene - 5 minutes, and then in alcohols 100°, 96°, 90°, 70° and 50° for 1 minute. Then the preparations were kept in distilled water for 10 minutes.
For enabling IRQ-unmasking antigen preparations were placed in CIT is atny buffer (rn) and heated for 8 minutes in the microwave power 800W at 100% power, and then 10 minutes at 60% power. Then samples were cooled to 25°C in citrate buffer (rn) and washed with distilled water 2 times for 5 minutes. The tissue sample was fortified with a hydrophobic pencil Dako Pen (Dako, Code S2002).
For inactivation of endogenous peroxidase for each drug was applied in 50 μl of 3%hydrogen peroxide and kept for 5 minutes. Then laundered Tris-buffer (rn) twice for 5 minutes. Then put 50 ál of 0.4% solution of casein, and incubated for 5 minutes, washed Tris-buffer (rn) 2 times, 5 minutes each.
Next, for each drug was applied primary antibodies - antibodies to MMR rabbit monoclonal IgG antibody (Epitomics, Clone ID: EP1254, Cat. N2551-1, Lot YG 11300 IP) working dilution of 1:100-1:250 and incubated in a moist chamber at 25°C and 100% humidity for 1 hour. Then laundered Tris-buffer (rn) 2 times, 5 minutes each. Then the preparations were applied in 50 μl of a solution containing 10% serum in Tris-buffer (rn), incubated in a humid chamber at a temperature of +25°C for 30 minutes. Washed Tris-buffer (rn) 2 times, 5 minutes each. Then put the secondary antibodies Novolink Polymer (Novocastra, REF=7112, Lot 711236)labeled with peroxidase, 50 ál, incubated in a humid chamber at a temperature of +25°To 30 minutes. Washed Tris-buffer (rn) 2 times, 5 minutes each. Added 50 μl of the substrate mixture containing 50 μl of 1.74% 3'3'-diaminobenzidine in 1 ml of 0.05% hydrogen peroxide Novocastra, REF=RE7105, Lot 710550), kept for 5 minutes. Washed with water. The slices they finished painting areas 0.02% solution of hematoxylin for 30 seconds. Washed with water. Dehydrational consistently alcohols 70°, 90°, 96°, 100° for 1 minute. Then in toluene for 5 minutes. Placed in the enclosing environment Permanent Slide Mounting Medium (Novocastra, REF=7137, Lot 713708) under a glass cover. Microscopic examination was performed using a light microscope Nikon 80L
Established that the deaths that have developed within a few hours after myocardial infarction, recorded brightly coloured neutrophils in the blood vessels periinfarct zone (Figure 1, item A), as well as brightly coloured neutrophils in the area of infarction (Figure 1, item B).
In the period of limitation of infarct from 1 to 2 days recorded the degranulation of neutrophils, loss of staining of the cytoplasm of neutrophils. In parallel with this was fixed coloration of the extracellular matrix in the area of the heart that reflects the allocation of MMP-9 from neutrophils in the tissue (Figure 2, item C). In the case of deaths at a later date (3-30 days) was noted colouring cells fibroblast number in the border zone. The maximum intensity of staining noted at 7-14 day (Figure 3, item D).
The authors note a good reproducibility of the proposed method is staining of serial sections in different parties reached an identical re ulitity staining.
Thus, the proposed method allow to clearly differentiate the limitation period of the onset of myocardial infarction with pathomorphological study. This method can be used in medicine, namely in pathological anatomy.
How pathological determining the limitation of the onset of myocardial infarction, including the fixation of the tissue sample and placing it in paraffin, making slices, dewaxing and warming up, laundering in the buffer solution, incubation in a humid chamber with the reagent and processing developing agent, dehydration and concluding on Wednesday, characterized in that the reagent is used antibodies to the matrix metalloprotease 9 in a dilution of 1:100-1:250, duration of incubation with the reagent at a temperature of 25°C and 100% relative humidity is 60 minutes, and microscopic identification of the preparation of brightly coloured neutrophils in the blood vessels periinfarct zone and brightly colored neutrophils in the zone of infarction establish a period of limitation of infarct from 2 hours to 1 day, in identifying the degranulation of neutrophils and bright colors of the extracellular matrix in the infarction zone from 1 to 2 days, and when the detection of stained cells fibroblast number in the border zone of the infarct - from 3 to 30 days.
FIELD: food industry.
SUBSTANCE: method envisages the sample acid hydrolysis, the hydrolysate filtration and chromatographic separation with subsequent automatic identification and quantitative evaluation of amino acids content using an automatic analyser. The invention allows to determine amino acids in the food product proteins composition with amino acids content equal to nearly 0.1-3.5 g/100 g of the product (1.5-17 g/100 g of protein) with application of sequential elution of amino acids with a buffer solutions mixture and simultaneous detection of the components at two wave lengths being 440 and 570 nm.
EFFECT: acceleration of the process of amino acids isolation from the food product and determination accuracy enhancement due to losses decrease and highly sensitive material application.
SUBSTANCE: submersible end of bearing pipe is equipped with metering head with submersible end and circumferential side surface. Said metering head with submersible end is furnished with at least one transducer or inlet for samples chamber arranged inside this device. Note here that said circumferential side surface of bearing pipe or metering head with accommodates inlet extending through intake channel into forechamber arranged inside said pipe or metering head. Forechamber end opposite metering head submersible end has inlet extending into slag sampling chamber arranged inside the device on forechamber side opposite said submersible end.
EFFECT: high-quality samples, precise analysis.
13 cl, 3 dwg
FIELD: machine building.
SUBSTANCE: aspirator-dust sampler consists of a casing, a diaphragm pump with electric drive, a system to stabilise the volume speed of air pumping, systems to measure the volume of the pumped air, a sampling tube and a filter holder with a filter. The diaphragm pump is made as two chambers being set towards each other, rigidly interconnected and driven by an eccentric mechanism. The eccentric mechanism is installed on the electric motor axis so that the front diaphragm position of one chamber corresponds to the opposite diaphragm position of the other chamber. Suction of air into one chamber is accompanied by the discharge of air from the other chamber. Suction valves are set on the movable diaphragms, and the discharge valves - on the stationary chambers' casing. Both chambers are the walls of the air-tight pump casing connected to a suction branch pipe in which a rarefaction sensor is built-in. The two-chamber pump is placed in another external air-tight casing where air from the chambers is discharged to and one wall of which is replaced by a rubber diaphragm serving as a damper together with the inner casing space. An air mass flow metre is built-in in the other wall. The rarefaction sensor and the flow metre are connected to a motor mode control unit and to the unit for the data on air flow, volume of pumped air, weight of dust on the filter and dust concentration.
EFFECT: improved accuracy of sampling and measuring the pumped air volume, keeping and measuring the constant volume speed of air pumping through the filter with dust residues, increased reliability of aspirator performance both at dust sampling and in the course of operation, simplified valve design, simplified measuring procedure for pumped air volume reduced to standard conditions.
3 cl, 1 dwg
FIELD: testing equipment.
SUBSTANCE: prismatic sample has a prism shape, longitudinal and transverse planes of symmetry, two side ledges, arranged longitudinally, at the ends of the prism - support surfaces, and in its central part - surface of loading with a transverse test load. The prismatic sample is additionally equipped with inclined support surfaces arranged on side longitudinal ledges of the prism and characterised by angles of inclination to the longitudinal plane of the prism symmetry 5…20°.
EFFECT: simplification and reduction of cost of prismatic sample testing process with concentrators of mechanical stresses in complex stressed condition, provision of necessary accuracy of modelling of a type of stressed-deformed condition of structure material in focus of its damage.
2 cl, 4 dwg
SUBSTANCE: invention refers to a medical sampling container, particularly to a modified multifunctional sampling container. The container comprises a body (1), a lid (2) on a body (1) opening, a fixed rotating rod (4) mounted on the lid (2) and freely rotating about the lid (2), and a sampling spoon (5) placed at the bottom of the fixed rotating rod (4). The container comprises a mesh filter (3) placed inside the body (1) perpendicular to the lid (2), and a separator connected to the bottom of the mesh filter (3). The above mesh filter (3) and the separator divide the container body (1) on a pump-down chamber (11) and a pump-off chamber (12).
EFFECT: preventing laboratory contamination and contagion, as well as providing odour-control treatment in the laboratory.
7 cl, 3 dwg
FIELD: process engineering.
SUBSTANCE: set of invention relates to entrapment of biological particles suspended in fluid for preparation of biological specimens for cytological analysis. It relates also to preparation of biological specimens with the help of this device, to platform and system for multianalysis. This device comprises pipe with first and second ends. Note here that pipe first end is closed by filter membrane surface glued to pipe wall cross-section. It includes the piston composed by rod engaged with thrust element. Note also that said rod can slide in axis parallel with pipe wall. Besides, it comprises the unit of hydrophilic absorbent arranged in said pipe between filter membrane inner surface and piston thrust element. Proposed process comprises placing aforesaid device in vessel with fluid wherein suspended are biological particles, retaining said device in said vessel for time sufficient for entrapment of a portion of biological particles on filter membrane surface. Then, this device is removed from said vessel to collect trapped biological particles from membrane filter.
EFFECT: production of high-quality cytological preparations using simpler device.
18 cl, 11 dwg
FIELD: tobacco industry.
SUBSTANCE: invention relates to the field of elaboration of biocatalysts intended for usage as part of biological gas purification filters and may be used during conductance of laboratory experiments with samples of biocatalysts removing volatile components of natural tobacco raw material from air as well as for creation of selective conditions in the process of isolation and study of microorganisms constituting the biologically active components of the sais type catalysts. For the method implementation tobacco raw material is moistened with water, the moistened tobacco raw material mass is placed into the extractor and heated to a temperature exceeding that of water boiling with subsequent pumping of purified air through the extractor to produce the model gas-and-air mixture.
EFFECT: production of a highly efficient biocatalyst for deodorisation of gas-and-air emissions.
SUBSTANCE: method of determining the coefficient of heterogeneity of a mixture of hard-to-separate granular materials involves determining the number of samples, the minimum allowable weight of a sample, collecting samples of the mixture and components thereof. The samples are distributed in a uniform layer on a smooth surface and photographed. Pixel-by-pixel analysis of images of miscible components is performed to obtain histograms of distribution of pixels of the image on shades of gray with respect to the total number thereof, followed by determination of the threshold shade. Concentration values of the key component in samples of the mixture are then determined as a ratio of the number of pixels corresponding thereto to the total number of pixels of the image of the sample and the coefficient of heterogeneity of the mixture is then calculated. When calculating the value of the threshold shade, coordinates of the centroids of areas of the histograms of distribution of pixels of the components of the mixture are found and the value corresponding to the abscissa of the middle of the section between the centroids of the areas of the histograms is assigned the threshold shade.
EFFECT: simple and accurate method of determining the coefficient of heterogeneity of a mixture of hard-to-separate components with minimum time consumption.
SUBSTANCE: invention refers primarily to mine-mill industry in part of operating procedure and equipment development and may be used during pulp sampling in quality control system for products preparation for analysis. Pulp flow sampling method includes installation of slot-type sampling device to sampled pulp pipeline using mating flanges, pulp flow supply to reforming chamber of slot-type sampling device and taking primary sample using slot-type shutoff device. After pulp flow delivery to reforming chamber, pulp flow is reformed to horizontal average flow directed to expansion chamber. Pulp average flow is used for continuous taking of primary sample supplied through inlet chamber and sample outlet branch to feeding funnel of sample reducing module. Reduced sample taking is performed using cutoff-bucket, reduced sample from cutoff-bucket is directed to flow reducer, in which after mechanical mixing of flow on homogenisation disk flow is reformed to vertical down flow through homogenisation chamber, and then - to annular flow directed to reducing chamber walls outgoing from reducing disk, and controlled continuous taking of final reduced sample is performed from pulp annular flow.
EFFECT: obtaining final reduced accumulated sample with minimum error.
3 cl, 3 dwg
SUBSTANCE: system and method for ground material characterisation in a grinding system use an irradiation section through which at least a part of the ground material stream is fed and with irradiation means for irradiating the particles in the part of the stream with electromagnetic radiation; and a detection section for passage, having a detection means for detecting electromagnetic radiation emitted from the particles of the part of the ground material stream fed through the irradiation section The detection means comprises an imaging system and a colour image sensor for imaging the particles thereon using the electromagnetic radiation emitted by the particles. The colour image sensor comprises image elements for spectrally selective detection of the electromagnetic radiation imaged on the sensor image elements. The detection section comprises a luminous means or is made and arranged to detect particles of the ground material using a combination of transmitted and incident light.
EFFECT: high rate and accuracy of detecting properties of a stream of a grinding product.
26 cl, 3 dwg
SUBSTANCE: invention refers to a faecal sample collection and extraction device, particularly applicable for laboratory diagnostic analyses in a completely automated laboratory, namely for the recovery of one or more a substance sampled, and the diagnostic marker test for prevention and treatment. The faecal sample collection test tube comprises a hollow open-ended container, a first lid provided with a threaded rod for the faecal sample collection. The rod with the lid placed on the first end of the container is axially placed inside the container. The test tube comprises a partition in the middle of the container and dividing it on first and second compartments. It additionally comprises a gripper assembly integrated with a second end of the container opposite to the first end, and forming a retainer for gripping of automatic analysis machines for automated handling of the test tube.
EFFECT: technical effect is ensured by developing the advanced test tube enables stating the obvious unauthorised test tube manipulations effectively and immediately.
15 cl, 10 dwg
SUBSTANCE: neuropsychological testing aims at determining an analytic-synthetic thinking index; a depression index; a trait anxiety index; a state anxiety index; a mental alertness index; a psychomotor activity rate, and recording an electroencephalography to measure β1-rhythm and β2-rhythm. A diagnostic function F is calculated; the derived value is compared to a constant, and if F is more than the constant, the cognitive disorders accompanying encephalopathy caused by an effect of alcohol are diagnosed; if F is equal to or less than the constant, the cognitive disorders accompanying chronic mercury intoxication are diagnosed.
EFFECT: technique enables providing more accurate differentiation of the cognitive disorders accompanying toxic encephalopathy caused by the effect of mercury, and alcoholic encephalopathy.
2 tbl, 3 ex
SUBSTANCE: invention relates to forensic medicine and can be used to determining the prescription of death coming by the morphological changes of putrid adhesions. For this purpose, a forensic examination of a corpse involves sampling adhesions and a portion of abdominal muscle tissue from an attachment points of the adhesion. Then, histological sections are taken from the tissue samples and stained with haematoxylin and eosin and Masson and Zerbino trichrome. That is followed by a microscopic examination of the stained sections. If observing the cell loss in haematoxylin and eosin staining, while maintaining the Zerbino muscle cell staining, and collagen and reticular fibres staining in Masson trichrome, the prescription of death coming is stated within 7 to 10 days. Besides, if the examined tissues lose their ability for Zerbino myocyte staining, the prescription of death coming is stated within 10 to 14 days. If the reticular fibres are not stained in the sections with maintaining the collagen fibres staining in Masson trichrome, then the prescription of death coming is set more than 14 days.
EFFECT: method enables specifying the prescription of death coming up to 14 post-mortal days.
SUBSTANCE: X-ray image of a proximal one-third of femur and pre- and post-therapeutic patient's clinical state are analysed. The X-ray image is used to measure an average optical density (AOD) of soft tissues along an inner surface of a proximal femur to be aligned. If observing the AOD as compared to the pre-therapeutic level by more than 50% in a combination with intermittent slight pain syndrome, a response to the therapy is considered to be positive. The AOD decreased within 16 to 50% in a combination with persistent slight pain syndrome shows a satisfactory response to the therapy. If the AOD decreases by 15% and less in a combination with persistent pronounced pain syndrome provides stating a negative response to the therapy.
EFFECT: objective estimation of the manual therapy in the given group of patients.
6 dwg, 3 ex
SUBSTANCE: what is involved is an endoscopic assessment of a degree of oesophageal involvement. The oesophagus is stained with 0.3% Congo red 2-3 ml through a spray catheter with maximum air insufflation. A length of the stained oesophageal segment (h) from a Z line with the stain colour changed by blue-black is measured in cm. Stain completeness coefficient K of an oesophageal circle is visually estimated as 1 for complete staining, and as 3/4, 1/2, 1/4 for partial staining. The derived data are used to calculate an area of the oesophageal involvement (S) by formula: S=27πrhK, wherein r=1 cm. If the patient shows no endoscopic signs of oesophagitis, though has pathologically acidic reflux oesophagitis with S>3.14 cm2, a high risk of degree I reflux oesophagitis is predicted. The value S>9.42 cm2 in the patient degree I reflux oesophagitis enables predicting a high risk of a transformation into degree II reflux oesophagitis .
EFFECT: simple and information-bearing method enables the early detection of the patients relating to a risk of developing and progressing reflux esophagitis, provided prevention of developing and progressing organic changes of the oesophagus for the timely administration of a therapy.
2 cl, 3 tbl, 2 ex
SUBSTANCE: group of inventions refers to medicine. A biopsy system comprises: a visual display system for diagnostic imaging, a probe comprising a slip biopsy needle, a computer connected to a tracking system, the visual display system and an ultrasonic visual display system. A machine-readable medium comprises a computer executable code for implementing the stages of a method of using the tracking system for biopsy. The method for imaging involves the stages providing the diagnostic imaging of a target region covering a biopsy point. The tracking system matches with the diagnostic images. Ultrasonic images of the target region are formed during a biopsy procedure. The tracking system is used to obtain tracking data to localise at least one of: the probe, the biopsy needle and a needle guide during the biopsy procedure. The localisation is marked in the biopsy point on the ultrasonic images. The marked localisation is transferred from the ultrasound images onto the diagnostic images by reference to the tracking data and matching the tracking system with diagnostic images.
EFFECT: group of inventions enables providing more accurate mapping of the biopsy points.
15 cl, 14 dwg, 1 ex
SUBSTANCE: invention refers to medicine, namely recreation therapy, and may be used for human health improvement. That is ensured by traditional medical examination of the patient. The findings are used to draw up the rehabilitation program including body cleansing, intestine and liver purification, physical exercises. One week before the beginning of the rehabilitation program, the patient starts separate nutrition with preferential vegetable food and dairy products, limited consumption of salt, alcohol and sugar. One day before the rehabilitation program, a fasting day is kept with taking 1% kephir or fresh apple juice. Further, on the first two days of the three-day program or on the first four days of the seven-day program, the body is cleansed by keeping a juice diet. The juice diet contains fresh vegetable and fruit juices, herbal infusions and mineral water. Water is unlimited. On the first day of the cleansing program, the intestine is purified with the preparation Fortrans. Starting from the first day the liver and gall bladder are cleansed with using herbal and saline choleretics for four days of the 7-day program and for two days of the 3-day program. That is combined with paradoxical respiration by Strelnikova's technique and hydrotherapy. Physical exercises involve physical loads on a cardio-vascular machine, one training as prescribed by the doctor - yoga, pilates, chi gong, water aerobics or hypoxi capsule. Therapeutic massage and lymph drainage massage follow. Then body-detox, ozone therapy, collagenarium procedures are performed. Cold training procedures involve Linear Kneipp, saunas, swimming pool, turpentine bathes. Bathing and cold training procedures are followed by pilling and algae wraps. From the 5th day of the 7-day program, or from the 3rd day of the 3-day program, the diet involves water cereals, vegetable and fruit salads, vegetable fast soups.
EFFECT: method provides effective patient's health recovery, namely weight loss, health improvement, blood value normalisation, formation behavioural model of health preservation over a shortest possible period of time.
SUBSTANCE: invention refers to medical equipment, namely to a device and method for easy collection, dilution, mixing and dosing an analysis fluid in an isolated system. A sample suction and dosing unit comprises a container and a sampler. The container comprises a sealed chamber, limited at least from one side with a permeable element. The sealed chamber contains a fluid. The sampler has an open-ended passage. At least a portion of the passage extends from a first end comprising a capillary passage adsorbing the sample by a capillary action. The sampler contains a penetration unit penetrating inside the above permeable element so that the above passage is connected with the above sealed chamber after the permeable element has been perforated. The above canal is connected with the sealed chamber to allow for mixing the sample and fluid medium and dosing the mixed sample with the fluid medium from the device from the capillary passage. The method for sample suction and dosing with the use of the above device comprises the following stages: suction of a fluid sample into the above capillary passage, penetration into the above permeable element to interlock with the permeable element so that the above passage is connected with the sealed chamber, for actuation of the permeable element as a piston rod for mixing the sample and fluid medium and dosing of the mixed sample and fluid from the device through the capillary passage.
EFFECT: present invention may be used in a combination with a number of testers for chemical, biochemical or biomedical qualitative or quantitative analysis within clinical and hygienic studying.
24 cl, 23 dwg
SUBSTANCE: invention relates to field of medicine, namely to oncology. In order to predict efficiency of pre-operation radiotherapy of squamous carcinpmas of head and neck immune-enzyme analysis of TIMP-1 and TIMP-2 in blood serum is carried out. Level of MMP-2 and dimensions of primary tumour are additionally determined in accordance with international classification TNM. Discriminant functions Y1 and Y2, and efficiency of pre-operation radiotherapy is predicted on the basis of their comparison.
EFFECT: method increases accuracy and self-descriptiveness of prediction of efficiency of pre-operation radiotherapy of squamous carcinomas of head and neck due to assessment of the most informative parameters.
SUBSTANCE: invention relates to medicine, in particular to infectology, and deals with predicting development of vascular disorders in patients with influenza. For this purpose age of patients and term of observation are taken into account and values of ristomycin-aggregation of platelets in blood serum are determined. Obtained data are substituted into formulas: PC=28.92+A*0.35-B*0.26-C*2.18, where PC is prognostic coefficient of risk of development of vascular disorders 28.92 is constant of mathematical calculation for prediction of development of vascular disorders; A is patients' age group, where 1 is age of patients from 21 to 35, 2 is from 36 to 50, 3 is age from 51 to 65; B is term of observation: 1 is 1-3 days of disease, 2 is 4-5 days of disease, 3 is 6-8 days, 4 is 9-14 days from the onset of disease, 5 is one month from the onset of disease, C is value of ristomycin-aggregation of platelets in blood serum in seconds. PC value is used to predict development and expression of vascular disorders in form of tortuosity and non-uniformity of caliber of microcirculatory bed vessels in patients with influenza and after influenza. If Pc is larger than 0, but lower than 6, development of mildly expressed vascular disorders is predicted, if value is from 6 to 9 - development of moderately expressed disorders, with PC value from 9 to 12 - expressed vascular disorders.
EFFECT: method ensures identification and prevention of said changes in due time and makes it possible to perform their correction in justified way.
FIELD: medicine, clinical toxicology.
SUBSTANCE: at patient's hospitalization one should gather the data of clinical and laboratory values: on the type of chemical substance, patient's age, data of clinical survey and laboratory values: body temperature, the presence or absence of dysphonia, oliguria being below 30 ml/h, hemoglobinuria, erythrocytic hemolysis, exotoxic shock, glucose level in blood, fibrinogen and creatinine concentration in blood serum, general bilirubin, prothrombin index (PTI), Ph-plasma, the state of blood clotting system. The state of every sign should be evaluated in points to be then summed up and at exceeding the sum of points being above "+20" one should predict unfavorable result. At the sum of "-13" prediction should be stated upon as favorable and at "-13" up to "+20" - prediction is considered to be doubtful.
EFFECT: higher accuracy of prediction.
2 ex, 3 tbl