Combination of lycopene, polyphenol and vitamins for care of keratin materials

FIELD: medicine, pharmaceutics.

SUBSTANCE: invention relates to the field of cosmetology, namely to a cosmetic composition for peroral introduction, which contains a combination of lycopene, vitamin C, vitamin E and at least one polyphenol compound, obtained from pine bark, in which the ratio of weight content of polyphenol compound to the sum of weight contents of lycopene, vitamin C and vitamin E constitutes from 0.3 to 0.7, as s single active ingredient.

EFFECT: invention is intended for prevention and/or treatment of wrinkles in the area of eyes and mouth angles, small wrinkles, eye bags and dark circles under eyes.

22 cl, 2 ex, 11 tbl

 

The present invention relates to the field of food additives intended for skin care. Its purpose is also the use of a composition for oral and/or parenteral administration containing a combination of active ingredients designed to maintain and/or restore the biomechanical properties of keratin materials, such as connective tissue and, more specifically, of the skin. It relates also to a composition for oral and/or parenteral administration, representing a particular combination of these active ingredients.

The human skin consists of three layers: epidermis, dermis and hypodermis. The last layer consists essentially of a single type of cells, specializing in the accumulation and storage of fat in the adipocyte. The hypodermis is the energy reservoir of the organism.

The human epidermis is composed mainly of three types of cells: significantly prevailing keratocytes, melanocytes and Langerhans cells. Each of these cell types, their function contributes to the significant role played by the skin in the body.

The dermis gives the skin a solid support. In addition, she provides nutritional function. Basically it consists of fibroblasts and extracellular matrix, which, in turn, consists of collagen, elastin and a substance nazyvaemogo substance components synthesized by the fibroblasts.

In addition, there find leukocytes, Metacity and tissue macrophages. It also cross the blood vessels and nerve fibers. In normal skin, there are no abnormalities and no scars fibroblasts are at rest, that is not proliferate.

It is the collagen fibers give the skin strength. Collagen fibers are composed of fibrils bonded among themselves in such a way that it forms more than ten different structural types. His strength skin in a large part thanks to the confusion of collagen fibers, which are stacked one another in all directions. Collagen fibers give the skin firmness and tone of the skin and mucous.

Collagen fibers are continuously updated, but the update decreases with age, which leads to the thinning of the dermis. The thinning of the dermis can lead to a pathological condition, for example, increased secretion of corticosteroid hormones or lack of vitamins (vitamin C in the case of scurvy). It is possible that external factors such as ultraviolet rays, tobacco and certain medications (eg, glucocorticoids, vitamin D and its derivatives), also affect the skin and the content of collagen.

Various factors cause the destruction of collagen with all the ensuing p. the consequences for the structure and/or the elasticity of the skin and/or mucous membranes.

Being very stable, collagen fibers sensitive to certain enzymes called collagenases. Destruction of collagen fibers leads to the fact that the skin becomes loose and wrinkled, and people who prefer to have his skin was smooth and tight, always tried to deal with it.

Collagenase are a family of enzymes called metalloproteinases (MMP), which in turn are members of a family of proteolytic enzymes (endoproteinase, or endopeptidase), which includes the zinc atom, the coordination associated with three cysteine residues and one methionine residue in the active center, and which destroy the macromolecular components of the extracellular matrix and basal membranes at neutral pH (collagen, elastin and the like). These enzymes, being widely distributed in the living world, are present in some quantity in normal physiological States, such as the growth of organs and tissue renewal.

Their overexpression in humans and their activation is associated with numerous processes, including pathological, which involved the destruction and rebuilding of the matrix. This causes either uncontrolled resorption of the matrix, or Vice versa, the state of fibrosis.

The family of metalloproteinases consists of several groups, well-defined with what tion of enzymes in terms of the structure of the substrate and specificity. Among these groups can lead collagenase, intended for the destruction of fibrillar collagen (MMP-1 or interstitial collagenase, MMP-8 or neutrophil collagenase, MMP-13 or collagenase 3), gelatinase, which destroy collagen type IV and all kinds of denatured collagen (MMP-2, or gelatinase A (72 kDa), MMP-9, or gelatinase B (92 kDa)), stromelysin (MMP-3), a broad spectrum of activity which aims to extracellular matrix proteins, such as glycoproteins (fibronectin, laminin), proteoglycans and the like, or membrane metalloprotease.

In addition, some proteoglycans, for example, belong to the family of small proteoglycans, rich in leucine (Small Leucine-Rich addition proteoglycans, SLRP), represent an interesting target from the point of view of prevention of such negative phenomena as the aging and deterioration of the mechanical properties of the skin. In fact, SLRP directly involved in fibrillogenesis and hydration peripapillary space, in particular, SLRP contribute to enhancing the bioavailability of some growth factors such as TGF-P. Among SLRP may be mentioned decorin, lumican, fibronodular, biglycan. On the other hand, some immunohistochemical observations reveal a decrease in the accumulation of biglycan in elderly skin. In addition, it is noted that the reduction of lumican and fibronodular leads to deterioration of the of fibrillogenesis collagen, and breach of architecture fibers. Therefore, proteoglycans SLRP family play a fundamental role in the architectural organization of the structures of the skin.

Finally, SLRP not only sensitive to the action of metalloprotease, but also to the proteolytic action of aggrecan, or ADAMTS (A Disentegrin And Metalloprotease with Thrombospondin type I repeat. Some members of this new family of metalloprotease, in particular, ADAMTS1 and 4 identified in the skin, a ADAMTS4, as you know, splits decorin.

Prolonged exposure to ultraviolet radiation, in particular ultraviolet radiation type A and/or B stimulates the expression of collagenases, in particular MMP-1. This is one of the components of the photo-induced aging of the skin.

On the other hand, the menopause, the main changes affecting the skin, are the reduction of collagen and thickness of the skin. This causes in women during menopause thinning of the skin and mucous membranes. With women there is a sense of papyrus skin or tightening of the skin and marked the deepening of the shallow and superficial wrinkles. The skin becomes rough to the touch. Finally, decreasing the softness of the skin.

Finally, overweight people, more specifically, during weight gain, adipocyte tend to a rapid increase in volume (storage of increasing amounts of lipids). When this fat lobules gradually rustagi which are stated, what causes stretching of the connective tissue strands parallel to each other and perpendicular to the surface of the skin. Heavy pressure from the adipocytes into the dermis, quickly causes deformation of the surface of the skin. This phenomenon, known as cellulite, is that the skin has an orange peel appearance. In clinical terms cellulite is a change of texture subcutaneous and surface tissue, which, in particular, differs as follows:

- the skin is generally thicker,

- a thicker skin,

- more sensitive skin, which may even, depending on the stage of development of cellulite, to be painful with the feeling, and/or

- cutaneous tissue of the less mobile due to loss of adhesion and fusion of the deep layers of the skin.

In addition, this phenomenon is more noticeable in women, because they have thinner skin with vertical connective fibers, in contrast to men's skin, where the connective tissue fibers are tilted and overlap between them.

Cellulite, which is often complicated by overweight and obesity, most often localized at the level of the pelvis and lower extremities ("breeches" or "clown pants"). Such changes can even cause permanent cicatricial changes.

Hypertrophy of the connective tissue at the level of the skin is accompanied by tension network of fibers, h is about leads to functional disturbances in the cells located there. In fact, this excessive tension interferes with cellular metabolism, venous blood circulation and lymph drainage, because it is compressed vessels, so that this state is prone to self-maintenance. In the end, the fibers degenerate, and the skin loses its basic structure.

Biologically under normal tension skin fibroblasts actively synthesize collagen, elastin and glycosaminoglycans - the key molecules that are involved in strengthening the tissues of the skin. Similarly, adipocytes, lipid, also put pressure on the dermis that causes sverhproizvoditelny collagen, up to fibrosis.

In contrast, when the weight loss, particularly when following a low calorie diet, fast unloading of adipocytes leads to a decrease in pressure provided by the hypodermis on supporting tissues. Consequently, since the dermis is not under pressure, the skin gradually loses its connectivity, reduces the adhesion of fibroblasts with collagen, reduces neocollagenesis, reduces the tension of elastin fibers, depolymerized proteoglycans, etc. While fibroblasts, weaker interacting with the fibers of the extracellular matrix, no longer receive from their environment signals to the increased activity and reparations that trigger the synthesis of the basic macromolec the l dermis. Moreover, fibroblasts, no longer receiving signals from its fibrillar environment, secrete matrix metalloprotease (MMP) enzymes that cause the destruction of the fibrous structures. This significant decrease metabolism in fibroblasts, followed by destruction of the fibers metalloprotease, eventually leads to the deterioration of viscoelastic, or biomechanical properties of the skin (loss of elasticity, tone, elasticity and so on).

From the above it becomes clear the importance of collagen and glycosaminoglycans in the tissues, particularly the skin and/or mucous membranes, and how important it is to prevent their destruction in order to prevent loss of viscoelastic, or biomechanics properties of the skin (loss of elasticity, tone and so on), whether it is associated or not associated with chronobiological or photoinduced aging.

The purpose of the present invention is precisely to propose a new combination of active ingredients that are useful to alleviate and/or prevent violations of the biomechanical properties of the skin and, thus, the severity of skin disorders that are directly related to such violation, in particular, concomitant disorders, thinning of the skin and/or destruction of collagen fibers, while the latter leads to the fact that the skin becomes loose, flabby and/or wrinkled, and PR is correspond to fight the present invention.

More specifically, the interests of the present invention relate to the prevention and/or treatment, orally or parenterally, symptoms of deterioration viscoelastic, or biomechanical properties of the skin. More specifically, the present invention consists in maintaining and/or restoring the biomechanical properties of the skin.

More specifically, the authors of the present invention have found that the combination of lycopene, vitamin C, vitamin E and at least one polyphenolic compounds, in particular polyphenol compound derived from pine bark, oral and/or parenteral administration has a beneficial effect on keratin materials, in particular, on the part of the dermis, and allows you to fight with impaired biomechanical properties of keratin materials and/or beneficial effect, in particular, to maintain and/or restore the biomechanical properties of the keratin material, in particular connective tissue, more specifically, of the skin.

Lycopene is a natural pigment found in ripe fruit, particularly in tomatoes. He belongs to the family of carotenoids, and its structure is similar to the structure of beta-carotene.

The role of lycopene in the fruit ripening known from the prior art. Lycopene is used in compositions for tanning, because it affects the synthesis Melanie is a (WO 97/47278), in compositions for the treatment of hair and/or acne due to activity against 5α-reductase (JP-2940964), as a tool against free radicals (JP-A-8-283136), as well as in compositions for the prevention and/or treatment of skin aging manifestations (EP 1090628).

Polyphenolic compounds have been known, in particular as antioxidants and are widely used in cosmetics. Also described their activity in relation to the prevention of cardiovascular diseases by oral administration. Some of them are used in compositions for external use, for example, in esterified form, as described in document FR 2706478 to make them fat-soluble properties to improve their resistance to oxidation.

It is known that vitamin C (ascorbic acid) is capable of stimulating the synthesis of collagen, preventing, as a cofactor, autoactivate lysine and prolylhydroxylase and increasing the synthesis of mRNA procollagen. It is also known that ascorbic acid (vitamin C) stimulates the synthesis of elastin and treats wrinkles.

Has already been proposed a combination of the above compounds with other active ingredients in the compositions for oral administration, for example, food supplements, the above compounds were served, in particular, to improve the physiological condition of the patient is s, as expressed, in particular, in the best resistance cardiovascular, rheumatic and circulatory diseases (US 6,605,296), as well as to improve skin condition in terms of smoothness and softness.

From the document WO 02/34210 known application of at least one carotenoid and vitamin C for the treatment of skin aging manifestations.

From the document WO 2006/000226 known composition for oral administration, contributing to the treatment of skin aging manifestations, vitamin E, vitamin C and white tea extract, as well as, in some cases, antioxidant, extracted from grape seeds, tomato, soybean and/or chamomile, and in this extract contained glycosaminoglycan and at least one transition metal.

Finally, the described composition for oral administration, which includes, as active ingredients contains a blend of vitamins, carotenoids. selenium, zinc, amino acids, glycosaminoglycans, blueberry extract and Pycnogenol®, and such a composition is proposed for the treatment of wrinkles and/or decreased skin elasticity (Segger et al. Journal of Dermatological Treatment (2004), 15, 222-226).

Generally speaking, loss of skin elasticity is a result of changes in the network of elastic fibers of the dermis, as well as reducing levels of glycosaminoglycans, which control the flow of the interstitial fluid surrounding fibers in the dermis. For this reason, p is losing skin elasticity is not specific to one group of the population and may manifest, starting from a very young age.

In turn, the present invention more specifically relates to the protection of the skin or even improve its tone, elasticity, softness and/or density.

The lack of tone, elasticity, softness and/or density of the skin is often the result of aging, but also of fatigue. Thus, such a disadvantage can serve as a direct basis for the occurrence of such manifestations of aging, such as fine and deep wrinkles that usually appear, starting from the age of 30, as well as manifestations, not directly connected with aging, but still observed more frequently in adults, such as bags and circles under the eyes.

In terms of their biological mechanism wrinkles are the result of loss of muscle tissue or permanent stretching of the muscle tissue, loss of adipose tissue, permanent effects of gravity and loss of bone and cartilage tissue facial area. Flattening the connection of the dermis with the epidermis, as well as reduced levels of collagen IV are considered as factors influencing the formation of wrinkles.

From the histological point of view, the epidermis in this Trofimova, and this manifestation is different from ptosis of the skin tissue observed in the loss of skin elasticity.

Unexpectedly, the authors of the present invention found that it is possible to treat these skin manifestations, is it deep and fine wrinkles, bags and circles under the eyes, effectively stimulating the biomechanical properties of the skin through the introduction of a very specific composition.

To the authors ' knowledge of the invention, the effectiveness of these compounds in oral and parenteral administration for the prevention and/or treatment of disorders of biomechanical properties of the component parts of the keratin material, in particular, the dermis, the previously described was not.

On the other hand, it is known that the treatment of external funds allows you to deal with skin manifestations, associated in particular with aging. However, the declared outside the active ingredients are not always active, since they are not absorbed into the skin. In addition, topical applications may be distributed unevenly over the area to be treated, and require careful and repeated application. In some cases, they can cause side effects on the skin, and discomfort.

In contrast, oral administration has the advantage that affects the entire skin, including its deeper layers (dermis and hypodermis), while being quick and easy method. In fact, metabolites and other active nutrients are distributed, in particular, inside the matrix of the dermis with blood flow. Oral or destination in videopage patch also have this advantage, what are quick and easy ways of reception.

The combination according to the present invention more specifically allows you to maintain and/or restore properties such as tone, elasticity, softness and/or density of the skin.

The present invention relates, in particular, to the use of such combination for the prevention or treatment of skin manifestations, such as wrinkles and fine lines and/or for preventing and/or treating manifestations of fatigue, such as bags under the eyes, around the eyes and dull, devoid of freshness to the skin, preferably for prophylaxis and/or treatment of bags under the eyes.

In a preferred embodiment, the combination according to the present invention can prevent and/or treat such symptoms of fatigue, as bags under the eyes, around the eyes and dull, devoid of fresh leather.

It also allows to improve the quality of the nails.

This combination is especially interesting in relation to the beneficial effects on maintaining and/or restoring skin elasticity in women during menopause. It was also proven to increase cellular activity of the dermis and the best hydration matrix gel in the composition of the intercellular matrix with orally administered nutritional supplements containing this combination.

Now izaberete is s also applies to the use of a combination of lycopene, vitamin C, vitamin E and at least one polyphenol compound derived from pine bark, as active ingredient to obtain a composition for oral and/or parenteral use in order to accelerate healing.

In particular, compositions according to the present invention is particularly useful for maintaining and/or restoring properties, such as tone, elasticity, softness and/or density of the skin.

In addition, the present invention is intended for oral use for cosmetic purposes a combination of lycopene, vitamin C, vitamin E and at least one polyphenol compound derived from pine bark, as active ingredients for improving the quality of nails.

The present invention relates also to a composition for oral and/or parenteral administration containing as the sole active ingredient combination of lycopene, vitamin C, vitamin E and at least one polyphenol compound derived from pine bark.

In addition, it relates to a composition for oral and/or parenteral administration containing a combination of lycopene, vitamin C, vitamin E and at least one polyphenol compound derived from pine bark, in which the ratio between the mass content of polyphenol the CSOs connection to the total mass content of lycopene, vitamin C and vitamin E is from 0.05 to 1, 2, in particular from 0.2 to 1, and in particular from 0.3 to 0.7.

Preferably, such a composition is represented also in the form of soft capsules, gelatin capsules, gels, dry or liquid emulsions, tablets, dispersible powders or vials of drinking the contents, or active products, such as yoghurts, drinks, etc.

Compositions according to the present invention are used, in particular, to maintain and/or restore properties such as tone, elasticity, softness and/or density of the skin.

In particular, the combination of lycopene, vitamin C, vitamin E and at least one polyphenol compound derived from pine bark, is used to obtain a composition for oral and/or parenteral administration, intended for the prevention and/or treatment of skin manifestations associated with violation of the above properties.

Under "viscoelastic, or biomechanical properties of the skin in the framework of the present invention refers to the properties of elasticity, tone, elasticity and/or softness of the skin.

Under "skin disorders" refers to any change in the appearance of the skin, such as wrinkles, particularly around the eyes and in the corners of the mouth, fine lines, withered skin, loose skin, dull and devoid of freshness of the skin, thinning skin, bags under the eyes, skin with the gasket, the lack of elasticity and/or tonicity of the skin, as well as any internal changes in the skin that do not affect the appearance, for example, any internal degenerative changes in the skin, in particular, the destruction of collagen fibers due to ultraviolet radiation.

Under the "manifestations of fatigue" refers to any change in the appearance of the skin caused or aggravated by significant fatigue, lack of sleep and/or stress, for example, bags under eyes, under eye circles or dull, devoid of fresh leather.

Under "skin disorder caused by weight loss or a low-calorie diet" refers to any change in the appearance of the skin, for example, the faded appearance of the skin, which may be more or less pronounced due to weight loss.

Under "skin disorder caused by cellulite" refers to any change in the appearance of the skin, for example, sickofitall or "orange peel", which can be more or less pronounced in areas of excessive accumulation of weight, such as thighs, arms or abdomen.

In the framework of the present invention it is understood that "oral administration for cosmetic purposes" means the use of funds designated oral, with these funds, for example, in the form of supplements or active food products, as provided above in the case of oral p is imeneniya, affect the skin, improving the aesthetics and comfort or beauty, in particular, to protect, maintain in good condition, its changes and, in particular, decoration.

In the framework of the present invention, the term "parenteral administration is intramuscular injection, intravenous injection, as well as to the designation in the form of adhesive tape systemic action. In other words, this term applies to any route of administration other than oral, in which the active substances are released into the bloodstream.

The purpose in the form of adhesive tape system is the preferred method of parenteral administration. Adhesive tape, providing only a local effect, not relevant to the present invention.

Under "PCI device", "sticky patch" or "system percutaneous actions in the framework of the present invention, refers to any system providing active or passive release of the active substance through the skin, i.e. providing transfer thereof through the skin with admission to the General systemic circulation.

LYCOPENE

Lycopene used according to the present invention may be of natural or synthetic origin. Lycopene natural origin implies lycopene in pure form or in the ideal solution, any concentration, derived from a natural product, such as a plant extract, in particular tomato. Lycopene synthetic origin implies lycopene in pure form or in the form of a solution of any strength, obtained by chemical synthesis.

Lycopene natural origin can be made on the basis of natural material obtained from whole plants grown in vivo or from cultures grown in vitro.

Under grown in vivo refers to any culture of classical type, that is grown in soil, outdoors or in the greenhouse, and without soil.

By culture in vitro is a set of techniques known in the art, which allows artificial way to get a plant or plant part. The selection pressure imposed by the physico-chemical properties during the growth of plant cells in vitro, allows to obtain a standardized material available throughout the year, unlike plants, cultivated in vivo.

Preferably according to the present invention uses the plant material obtained in culture in vivo. Very preferably according to the present invention uses a tomato extract rich in lycopene.

Lycopene is also present in melon, guava and grapefruit.

Any method of extraction known in the art, can the be used to obtain lycopene, used according to the present invention.

Lycopene can be represented in the form of aqueous suspensions. For this reason, you can use the powder to obtain a slurry in cold or hot, for example, such as that marketed under the name of Lyc-o-Mato CWD® (Lycored).

As an example, according to the present invention can be used rich in lycopene extract from tomato, marketed under the name of LycOMato® (Lycored)containing, for example, from 6 to 10% of pure lycopene.

Any other more complex ingredient on the basis of lycopene can also be used to implement the present invention.

Thus, under more complex ingredient refers to the original composition comprising lycopene and whey protein. This original composition, in particular described in document WO 01/91588. This original composition is also called lactoscope. This ingredient is used in food additive in example 1. It is of interest that increases the bioavailability of lycopene and/or those that can easily be included in the composition of food supplements (in the form of bags, gelatin capsules, tablets, coated tablets, soft capsules, and the like).

The amount of extract used according to the present invention, of course, depends on the desired effect and can therefore vary in a wide range of the zone.

To indicate the order of magnitude, you can use lycopene in terms of a pure substance in a quantity of 0.0001 to 50 wt.%, preferably from 0.001 to 10 wt.%, more preferably, in quantities of 0.05 to 0.2 wt.% in relation to the total weight of the composition.

For example, if the composition is primarily aimed at prevention and/or treatment of such manifestations of fatigue, as bags under eyes, under eye circles, dull, devoid of fresh skin, you can use lycopene in terms of pure substance in an amount of from 0.001 to 1 wt.%, in particular, 0.005 to 0.1 wt.% in relation to the total weight of the composition.

Of course, specialist, if he uses lycopene in the form of a solution, for example, the herbal extract will be able to calculate the amount of solution, which he uses to obtain the composition so that the final amount of lycopene in the composition corresponded videopreteen quantities.

VITAMIN C

According to the present invention, vitamin C, or ascorbic acid and/or its analogues can be used individually or in mixtures of any nature and in any ratio and can be of natural or synthetic origin.

Ascorbic acid is commonly represented in the L-form, because in this form it occurs in rastitel the m world.

The amount of vitamin C, applicable according to the present invention, of course, depends on the desired effect and can therefore vary within a wide range.

To indicate the order of magnitude, in the composition according to the present invention, the vitamin C in terms of a pure substance can be present in an amount of from 0.0001 to 50 wt.%, preferably, from 0.1 to 10 wt.%, more preferably, in quantities of 3 to 6 wt.% in relation to the total weight of the composition.

For example, if the composition is primarily aimed at prevention and/or treatment of such manifestations of fatigue, as bags under eyes, under eye circles, dull, devoid of freshness to the skin, the vitamin C in terms of a pure substance can be present in an amount of from 0.001 to 5 wt.%, in particular, from 0.05 to 1 wt.% in relation to the total weight of the composition.

Of course, specialist, if he uses vitamin C in the form of a solution, for example, the herbal extract will be able to calculate the amount of solution, which he uses to obtain the composition so that the final amount of vitamin C in its composition corresponded videopreteen quantities.

VITAMIN E

Vitamin E can be represented in the composition for oral and/or parenteral administration in the amount of from 0.0001 to 50 wt.%, predpochtitel is about, from 0.1 to 10 wt.%, more preferably, from 0.5 to 2 wt.% in relation to the total weight of the composition.

For example, if the composition is primarily aimed at prevention and/or treatment of such manifestations of fatigue, as bags under eyes, under eye circles, dull, devoid of freshness to the skin, vitamin E can be present in an amount of from 0.001 to 5 wt.%, in particular, 0.005 to 1 wt.% in relation to the total weight of the composition.

POLYPHENOLIC COMPOUND

Polyphenolic compounds represent a large family of substances that are very widespread in the plant Kingdom. In plants they are found everywhere, from roots to fruits. Among the different classes of polyphenols can be noted, in particular, flavonoids, proanthocyanidins, lignans, lignins, stilbene, coumarin. So, polyphenolic compound, used in the context of the present invention, may be represented in all the above forms.

Polyphenolic compounds, in particular, can be obtained from plant extracts, chosen among extracts of green tea, grapes, for example, Vitis vinifera; pine, in particular pine bark; Apple, blueberry, hops, guava, cocoa, timber, such as chestnut, oak, horse chestnut, hazel.

The term "polyphenol compound" in the context of the present invention also extends the and the plant extract, rich in polyphenolic compounds.

Flavonoids constitute a major group of polyphenols.

With regard to catechin polyphenols, they are a subgroup of flavonoids, which also includes flavones, flavanone, flavonols and anthocyanins.

Specifically polyphenolic compound is a catechin polyphenol, as defined below.

Subgroup catechin polyphenols includes a set of connections is usually allocated from plants such as cocoa, tea, grapes and grape by-products; pine (Pinus maritima), cereal, some fruit; and having varying degrees of polymerization.

The basic monomer, also referred to as catechin, or catechol is a 3,5,7,3',4'-pentahydroxy-2,3-dihydro-2-phenylpropen, which can exist in CIS - and TRANS-forms; epicatechin is its isomer and also can exist in CIS - and TRANS-forms.

The catechin polyphenols mean as various isomers of the base monomer and oligomers (proanthocyanidin) or polymers (tannins).

More specifically, the catechin polyphenols that have application in accordance with the present invention, are selected from the group comprising: catechin, epicatechin, gallocatechin, epigallocatechin, and their salts, esters and/or derivatives thereof in Monomeric or oligomeric form.

If oligomers are used, they eleutherodactyline from 2 to 14 of monomer units, in particular, from 2 to 10.

Preferably, the degree of polymerization less than or equal to 5.

In particular, the compounds are applied, usually called proanthocyanidine or procyanidolic, also called precursors of anthocyanins or procyanidolic oligomers (PCO). After oral or parenteral intake of these oligomers are partially destroyed after adsorption with the formation of monomers. Such polyphenols can be associated with sugars, for example glucose, galactose, Ramezay, the galacturonic acid.

In particular, in the present text under catechin polyphenols refers to the mixture of monomers and various oligomers containing from 2 to 14 of monomer units as defined above in any proportions.

Among the widespread dimers family procyanidolic whose use is particularly preferred within the present invention, it is possible to bring procyanidin B1, procyanidin B2, procyanidin B3, and procyanidin B6 or B7.

Procyanidins B1, B2, B3 are present in plant extracts of pine bark, cocoa, Apple, bilberry, horse chestnut, hops, guava and hazel. Thus, the composition according to the present invention contains, preferably, one of these plant extracts.

Polyphenolic compound present in the composition according to the present invention, is received from the bark of a pine.

This polyphenolic compound derived from pine bark, different, preferably, the content of phenol trimers from 5 to 25 wt.%, preferably, from 10 to 20 wt.% in relation to the total weight of polyphenolic compounds. In addition, it is, preferably, different content of polyphenolic dimers at least 5 wt.% or from 5 to 25 wt.%, preferably, at least 10 wt.% or from 10 to 20 wt.% in relation to the total weight of polyphenolic compounds.

Polyphenol compound derived from pine bark, also preferably contains from 2 to 15 wt.%, for example, from 5 to 10 wt.%, phenolic acids type of ferulic acid, para-coumaric acid, caffeic acid and protocatechuic acid, relative to the total weight of the polyphenolic compounds.

Thus, a polyphenolic compound, particularly preferred for use in the present invention may have the following characteristics:

Analysis/scoreValue
Loss on drying≤5,0%
Sulphate ash≤0,4%
Water-insoluble substances (1% solution, T=37°C)≤5,0%

Insoluble in tetrahydrofuran (THF) substance (1% solution, T=20°C)≤1,0%
pH (4% aqueous solution, T=20°C)2,5-4,5
Polyphenolic trimers10-20%
Polyphenol dimer10-20%
Taxifolia + Taxifolin glycoside>3%
The content of phenolic acids(1)2-15%
(1) ferulic + para-coumaric + protomateria + caffeic acid.

So, in one of the embodiments of the present invention in terms of its first and second objects, a polyphenolic compound derived from an extract of Maritime pine. This extract of Maritime pine is described, in particular, in the article: "A review of French Maritime Pine Bark Extract (PYCNOGENOL®), a herbal medication with a diverse clinical pharmacology", P. ROHDEWALD, International Journal of Clinical Pharmacology and Therapeutics, Vol.40, No. 4, 2002 (158-168).

The composition according to the present invention preferably contains polyphenolic compound in amounts of from 0.0001 to 50 wt.%, preferably from 0.001 to 10 mass%, even more preferably from 0.5 to 2 wt.% in relation to the total weight of the composition.

For example, if the composition is focused more on prevention and/or treatment of such manifestations of fatigue, as bags under eyes, under eye circles and dull, devoid of freshness skin polyphenolic compound may be present in an amount of from 0.001 to 5 wt.%, in particular, from 0.1 to 1 wt.% in relation to the total weight of the composition.

As illustrated by the following examples, the authors present invention proved that the composition containing lycopene, vitamin C, vitamin E and polyphenol compound derived from pine bark, in the required proportions, can beneficially affect the matrix of the dermis, improving cellular activity of the dermis, improving the quality of the intercellular matrix of the dermis and improves the hydration of the matrix gel in the intercellular matrix.

Thus, the composition according to the present invention is designed to combat skin aging process and, specifically, is designed to maintain and/or restore the biomechanical properties of the skin.

Thus, as has been clarified previously, the composition according to the present invention is useful, in particular, to maintain and/or restore properties such as tone, elasticity, softness and/or density of the skin.

Therefore, to the position according to the present invention is intended, in particular, for the prevention and/or cosmetic treatment of skin disorders caused by aging over time, in particular, Mature female skin before and after menopause and for the prevention and/or cosmetic treatment of skin disorders caused by aging under ultraviolet irradiation.

Thus, the composition according to the present invention is also suitable for the prophylaxis and/or cosmetic treatment of skin disorders caused by menopause.

Thus, according to one of the preferred embodiments, the present invention relates to the use of the composition according to the present invention, in cosmetics as a composition intended for the prevention and/or treatment of skin disorders caused by menopause.

The composition according to the present invention, particularly adapted for the prevention and/or cosmetic treatment of skin disorders caused by the loss of weight that occurs when losing or keeping a low calorie diet, such as sagging of the supporting tissues, loss of skin tone and softness of the skin.

The present invention relates also to the use of a composition according to the present invention as a composition intended to combat the loss of softness and/or skin tone.

As shown in example 1, the level of vitamins is s significantly increased, thus, the composition according to the present invention has a beneficial effect on the healing process.

The present invention also relates to the use of a composition according to the present invention in cosmetics as a composition intended for the prevention and/or treatment of occurrences of fatigue, such as bags under eyes, under eye circles.

In a preferred embodiment, lycopene presented in this composition in an amount of from 0.05 to 0.2 wt.%, vitamin C present in an amount of from 3 to 6 wt.%, vitamin E present in an amount of from 0.5 to 2 wt.%, and polyphenolic compound present in an amount of from 0.5 to 2 wt.% in relation to the total weight of the composition.

If the composition is more particularly intended for the prevention and/or treatment of occurrences of fatigue, such as bags under eyes, under eye circles skin, lycopene presented in this composition in an amount of 0.005 to 0.1 wt.%, vitamin C present in an amount of from 0.05 to 1 wt.%, vitamin E present in an amount of 0.005 to 1 wt.%, and polyphenolic compound present in an amount of from 0.1 to 1 wt.% in relation to the total weight of the composition.

According to the invention, the value of the ratio between the mass content of polyphenolic compounds and the sum of the mass concentrations of lycopene, vitamin C and vitamin E sostavlayut 0.3 to 0.7.

If the composition is more particularly intended for the prevention and/or treatment of occurrences of fatigue, such as bags under eyes, under eye circles, the value of the ratio between the mass content of polyphenolic compounds and the sum of the mass concentrations of lycopene, vitamin C and vitamin E is from 0.3 to 0.7, even more preferably from 0.4 to 0.6.

ADDITIONAL ACTIVE INGREDIENTS

In a preferred embodiment of the present invention may use a combination according to the present invention, together with others, at least one food anti-aging active ingredient, food, lighting the active ingredient, food menopausal active ingredient and/or active food ingredient that promotes weight loss.

Among the anti-aging food active ingredients can, in particular, to bring food antioxidants, nutrients, reducing the level of free radicals; cofactors internal antioxidant enzymes: vitamin A, carotenoids, xanthophylls, isoflavones, some minerals such as zinc, magnesium, copper, selenium; lipoic acid, coenzyme Q10, superoxide dismutase (SOD), as well as taurine. Among the anti-aging active ingredients can, in particular, to lead unsaponifiable fraction lipids the x extracts of vegetable origin, Aloe vera, native or hydrolyzed collagen seafood, vegetable oil or seafood rich in omega-3 and omega-6 fatty acids (including gamma-linolenic acid), etc.

Among photoprotection food active ingredients can, in particular, provide antioxidants and traps free radicals: vitamin A, carotenoids, xanthophylls, some minerals such as zinc, magnesium, copper, selenium; coenzyme Q10, superoxide dismutase, probiotics.

You can lead a nutritious ingredients, having the properties of hydration, as well as immunomodulation: probiotics, an extract of Polypodium leucotomos, vegetable oil or seafood rich in omega-3 and omega-6 fatty acids, including gamma-linolenic acid.

Among food ingredients active against clinical manifestations of menopause (eg, hot flushes and so on) can, in particular, to lead isoflavones, lignans, dehydroepiandrosterone (DHEA), extracts of wild Yam, sage, hops; calcium, magnesium, protein hydrolysates, plant oils or seafood rich in omega-3 fatty acids, etc.

Of course, in addition to polyphenol compound derived from pine bark, other polyphenolic compounds, for example, described above, can be included in the composition according to the present invention as dopolnitelnost.

Among the nutritional ingredients that promote weight loss, it is possible in particular to produce green tea, mate, horse chestnut, Cola, caffeine, theobromine, sinepin, bromelain, ephedra, bitter orange, hoodia, Garcinia, chitosan, vegetable fiber (cactus, apples, pineapple, etc.), fennel, black currant, meadowsweet, black radish.

COMPOSITION

The combination according to the present invention is administered orally or parenterally. Appropriate compositions can be presented in all the pharmaceutical forms commonly used in appropriate ways.

In the case of oral route of administration we can talk about the composition type food additives or active food product, and pharmaceutical compositions.

The composition according to the present invention can, in particular, to be presented in the form of soft gelatin capsules, gelatin capsules, gels, dry or liquid emulsions, tablets, dispersible powders, vials with content for ingestion or any other form known in the art. The composition also optionally may contain excipients in a suitable amount, such as colorants, sweeteners, flavors, fillers, binders, preservatives, etc.

In another preferred embodiment of the present invention, lycopene, vitamin C, VI is Amin E and polyphenolic compound can also be included in food matrices for the production of such active food as a food tile; fortified foods, such as vegetable and animal oils, Margarines, compact powders, fibers, and also in the form of an emulsion in the composition of the drinks.

Thus, the composition according to the present invention can be active food product.

The composition may contain other antioxidants, and other vitamins, minerals, approved for use in Europe in food additives in accordance with the Directive of CE 2002/46.

In the case of the introduction of parenteral composition may be presented in the form of solution for injection or patch system percutaneous procedure.

For information, dose of lycopene, which allows to achieve the objectives of the present invention will be selected depending on the desired effect. For example, can be assigned a dose of from 1 to 22 mg, for example from 2 to 11 mg per day, even from 4 to 7 mg per day.

Similarly, doses of polyphenolic compounds in the form of monomers or oligomers used to achieve the objectives of the present invention, can range from 6 to 75 mg, for example from 13 to 75 mg, in particular from 20 to 75 mg / day, more private way, from 30 to 50 mg per day.

In addition, doses of vitamin C, used to achieve the objectives of the present invention, can be from 10 to 105 mg, for example from 20 to 105 mg, in particular from 33 to 105 mg per day, preferably from 50 to 70 mg the day.

Finally, the dose of vitamin E used to achieve the objectives of the present invention, can be from 1 to 17 mg, for example, from 3 to 17 mg, in particular from 6 to 17 mg per day, preferably from 9 to 11 mg per day.

If the combination is more primarily directed to the prevention and/or treatment of manifestations of fatigue, such as bags under eyes, under eye circles, dull, devoid of freshness to the skin, preferably for the prevention and treatment of bags under the eyes, the daily dose of lycopene may be from 2 to 11 mg, and/or daily dose of vitamin C can range from 20 to 70 mg and/or daily dose of vitamin E can be from 3 to 12 mg and/or daily intake of polyphenolic compounds may range from 13 to 50 mg

In one of the options for implementing the present invention relates to a composition for oral administration containing a combination of lycopene, vitamin C, vitamin E and at least one polyphenol compound derived from pine bark, in which the ratio of the mass content of polyphenolic compounds to the sum of the mass concentrations of lycopene, vitamin C and vitamin E is from 0.3 to 0.7, characterized in that it presents in the form of soft capsules, gelatin capsules, gels, dry or liquid emulsions, tablets, dispersible powders, vials with content for ingestion or active food products is s, such as yoghurts, drinks, etc.

The present invention relates also to the use of the combination of ingredients in accordance with the present invention to obtain a composition for oral and/or parenteral administration, intended for the prevention and/or treatment of skin aging manifestations and/or fatigue, preferably, symptoms of fatigue, in particular, bags under the eyes.

In particular, the combination of lycopene, vitamin C, vitamin E and at least one polyphenol compound derived from pine bark, in particular, applicable to obtain a composition for oral and/or parenteral administration, intended for the prevention and/or treatment of skin aging manifestations associated with loss of elasticity, firmness, tone, softness and/or density of the skin.

Compositions according to the present invention can be cosmetic, dermatological or pharmaceutical.

In the sense of the present invention the cosmetic composition involves the composition might have on the skin effect of improving the appearance and comfort, as well as focused on beauty, in particular, to its protection, maintenance in good condition, change it, and, in particular, decoration. It can be presented in the form of food.

Now the image is giving will be illustrated in the following examples.

Example 1 a preliminary clinical study on the quantitative determination of the reference mRNA after administration of A food additive

Dietary Supplement A

- Dosage form: tablets in the shell

- Dosage: 2 tablets per day

- The composition of A food additive

Table 1
The accepted name of the ingredient/excipientComposition (mg/tab.)
NOURISHING INGREDIENTSLactoscope 2%(1)100,00
Vitamin C (90%)(2)28,9
The extract of the bark of the Maritime pine(3)13,3
Vitamin E(4)a 4.9
(1) 2% of lycopene.
(2) 90% L-ascorbic acid
(3) In accordance with the descriptions in the monograph USP28, Maritime Pine Extract, Appendix A (a).

Where:

- from 65 to 75% procyanidins;

is ≤8% loss during drying.

(4) In accordance with the descriptions in the USP monograph (VitaminE), Ph. Eur. 5 (RRR-alpha-Tocopheryl Hydrogen Succinate) and FCC IV (d-alpha-Tocopheryl Acid Succinate).

Dietary Supplement A preferably is designed for women in menopause, which is tested for loss of skin elasticity.

The purpose of clinical studies

To estimate the effect of A food additive in comparison with placebo on skin biomarkers that may be associated with changes in the biomechanical properties of the skin after two months of nutritional Supplement A.

Research methods

- Two-month study of 18 menopausal women for more than 2 years (9 in the group treated with A dietary Supplement and 9 in the placebo group) age over 50 years, not receiving hormone replacement treatment and obnarujivshih the lack of elasticity of the skin on the inner surface of the hand.

- Fence biopsies of 3 mm at t0 and T2 of the month, followed by extraction of total mRNA.

- Determination of the amounts of specific mRNA encoding proteins that may be associated with changes in the biomechanical properties of the skin.

Obtaining mRNA for use in RT-PCR (reverse transcription - polymerase chain reaction)

Both biopsies were crushed in liquid nitrogen (Mikro Dismenbrator S, B. Braun Biotech International), then total mRNA was isolated and purified by ultracentrifugation in a density gradient cesium chloride. The amount of purified mRNA was assessed by optical is oglosheniy (OD) at 260 nm (Nanodrop), and as a dedicated RNA was assessed by the ratio of OD260/OD280. Also assessed the integrity of the selected RNA using the device Bio Analyzer (Agilent). Stock solutions RNA was prepared with a concentration of about 1.25 ng/ál.

An estimate of the number of skin biomarkers

The concentration of RNA were normalized with 28S rRNA. RT-PCR was performed quantitatively by adding to each tube of known internal standard of known concentration, presents a synthetic RNA, which will be cotranscriptional and coamplification simultaneously with the desired mRNA. Samples corresponding to t0 and T2 each volonteri, were analyzed in the same series by electrophoresis on the same polyacrylamide gel. Statistical analysis was performed by one-way method t-test for paired samples comparing each group values at t0 and T2. The ratio T2/t0=1,00 meant that both values are comparable. The ratio T2/t0>1,00 reflected the increase in the number of study transcript. In contrast, T2/t0<1,00 reflected a decrease in the expression of the studied mRNAs.

Results / quantification of mRNA targets.

Table II:
expression of biomarkers to target after taking A food additive (versus placebo)
the T2/T0
PLACEBOFOOD
ADDITIVE A
paired t-testpaired t-test
(n=9)(n=9)
VIM1,02±0,36NS1,24±0,250,03
DEC1,33±0,58NS1,40±0,510,03
FEBMOD1,17±0,44NS1,48±0,570,02
LUM1,05±0,53NS1,40±0,300,002
XYL0,92±0,27 NS1,25±0,270,01
VIM: vimentin; DEC: decorin; LUM: lumican; FIBMOD: fibronodular; XYL: xylosyltransferase.

The group receiving the Supplement, the level of mRNA encoding vimentin was significantly increased (p=0.03). In cells mathematizing origin, such as fibroblasts and endothelial cells, vimentin is the major structural substance of intermediate filaments. This cytoskeletal network is directly involved in the mechanical functions of the cell. In particular, low expression of vimentin affects the healing process. Cells deficient in vimentin have, in particular, low mechanical strength, and reduced properties motility and contractility. In addition, vimentin is involved in the spatial organization of focal complexes in the organization of the actin-dependent fibers, as well as in interactions with the extracellular matrix. Some of these interactions directly affected by the aging of the skin, as in the case of the cells responsible for the control of migration, proliferation and reduction of cells and for the control of metabolic phenotype.

Thus, the study of mRNA encoding vimentin showed that dietary Supplement A beneficial effect on cyto is kehlet fibroblasts. This indicator increased cell activity.

On the other hand, and mRNA encoding decorin, detects the increased expression using the nutritional Supplement A (p=0.03), whereas the expression of this mRNA remains unchanged when receiving placebo. Decorin is a small glycosylated protein (proteoglycan), which belongs to the family of small proteoglycans, enriched with leucine (SLRP). Decorin presents throughout the dermis, but not in the epidermis. It is synthesized and secreted by fibroblasts. Its primary role is in the regulation of collagen fibrillogenesis. In fact, decorin promotes better adhesion of the collagen fibers: attaching itself to the fibers, it allows them to organize among themselves with the formation of collagen bundles. It is shown that the level decorin decreases with aging.

The results concerning decorin, showing that a dietary Supplement has A stabilizing effect on the collagen fibers and, consequently, to improve the quality of the intercellular matrix of the dermis.

After supplementation there is also increasing mRNA level of the other SLRP, in particular, lumican (p=0.002) and fibromyalia (p=0.02), which largely involved in fibrillogenesis and hydration peripapillary space.

Finally, the expression of another mRNA snackoperates when nutritional Supplement A. We are talking about an mRNA that encodes xylosyltransferase (p=0.01), which is the first enzyme of the synthesis of proteoglycans, such as chondroitin sulfate and dermatooncology in fibroblasts. Xylosyltransferase is an enzyme that starts glycosylation, i.e. the consolidation of sugars on the Central protein chains of proteoglycan. Next proteoglycans go in the extracellular matrix, where they form an aqueous gel by absorbing water molecules. This gel washes the cells of the dermis, as well as collagen and elastin. Proteoglycans play a role not only in the conservation of moisture, which gives the skin resistance to compression, but in the structure of the matrix and the transmission of information. The level of proteoglycans decreases with age, which causes destruction of the matrix gel. Dietary Supplement A, acting on xylosyltransferase, partly helps to restore the quality of this gel. Through better retention of water molecules such matrix gel allows the dermis to restore "bulked up" state and, thus, to improve the condition of skin surface.

Conclusion

The combination of the data received indicates that a food additive "A" has a General impact on the quality of the dermis:

1) increasing the level of metabolism in fibroblasts by enhancing transcription of a gene of vimentin is a key protein cytoskeletal;

2) improving odny gel of extracellular matrix, who washes the fibers and cells of the dermis by increasing transcription of the gene xylosyltransferase - the first enzyme of the synthesis of proteoglycans;

3) structuring of collagen fibers by increasing transcription of genes lumican, fibromyalia and decorin - proteoglycans involved in fibrillogenesis.

Example 2. Assessment of the effectiveness of A food additive in relation to biomechanical properties of the skin in comparison with placebo.

Food additives A and its dosage corresponds to those shown in example 1.

The goal of clinical research: study of the effects of nutritional supplements on A change in the biomechanical properties and microrelief of the skin.

Research methods

Single center, double-blind study on two groups, one of which received a placebo. To ensure better comparability of the two groups was conducted, the enforcement procedure of randomization.

In this study, a total duration of 6 months participated 72 volunteer women aged 40 to 65 years, meeting the requirements of inclusion and exclusion.

Dietary Supplement A: n=49.

Placebo: n=23.

The effectiveness of A food additive were evaluated:

- dermatologist using various photographic atlases,

- dermatologist clinical 6-point rating scale from 0 to 5. The better assessed the condition or indicators of the e l e C the closer the score was to 5 and Vice versa;

by instrumental studies (Torquemeter®and analysis of the images on the prints of the skin);

by self - assessment survey volunteers.

The measurement instrument Torquemeter®

Torquemeter®is a non - invasive device. The measuring head consists of rolling a Central disk with a diameter of 20 mm and a stationary circular plate.

This device was fixed on the skin with the help of stationary concentric double-sided adhesive tape. The angle of rotation of the Central disc was measured angular sensor with very high resolution. When applying the measuring head of the Central disc rotates around its axis. Then the restriction of the rotation angle of the Ue is superimposed on the area of skin between the movable Central disk and a fixed peripheral ring (rapid deformation). Then the angle of rotation is gradually increased until the angle of the Uv.

After stopping torsional moment, the skin returns to its original state in two stages: rapid deformation, Ur) and slow to the starting position.

Specific zone, measured, marked by circles. Measured parameters Ue, Uv, Ur.

Graphical analysis of skin prints.

A negative effect of wrinkles on the skin surface was obtained by using silicone rubber. For image analysis, this size is the current covered side light in which wrinkles were shadows.

Analysis of the wrinkles was carried out using the program Toposurf.

Results

Assessment of skin with photographic atlases.

Table 2:
The change of skin condition - A Food additive
OptionsT0 (mean ±SD)T12 (mean ±SD)T12/t0 Test tuks for multiple comparisonsT24 (mean ±SD)T24/T0 Test tuks for multiple comparisonsAnalysis of variance
The depth of wrinkles "goose paws"3.3V±1,12,6±0,9Improving P=0.0032,8±1,0Improving P=0,045Improving P=0.004
Bags2,4±1,22,0±1,4NS1,8±1,2Improving p=0,034Improving p=0,042
Wrinkles in the corners of the mouth/td> 2,7±1,7is 1.9±1.7Improving p=0,048is 1.9±1.7NS (trend, p=0,055)Improving p=0,027
SD: standard deviation

Table 3:
The change of skin condition - Placebo
OptionsT0 (mean ±SD)T12 (mean ±SD)T12/t0 Test tuks for multiple comparisonsT24 (mean ±SD)T24/t0 Test tuks for multiple comparisonsAnalysis of variance
The depth of wrinkles "goose paws"3.3V±1,32,8±1,2NS2,7±1,1NSNS
Bags1,9±1,11,9±1,2NS2,0±1,3NS NS
Wrinkles around mouth2,5±1,51,6±1,5NS2,2±1,8NSNS
SD: standard deviation; NS: not statistically significant

In the treated group, A dietary Supplement, the depth of wrinkles "goose paws" statistically significantly decreased since 12 weeks. The nutritional Supplement is also A statistically significant decrease in wrinkles at the corners of the mouth, since 12 weeks, as well as bags under the eyes, since 24 weeks. These parameters did not change in the placebo-treated group.

Dermatological changes on the clinical rating scale

Table 4:
The change of skin condition - A Food additive
OptionsT0 (mean ±SD)T12 (mean ±SD)T12/t0 Test tuks for multiple comparisonsT24 (mean ±SD)T24/t0 Test tuks for multiple comparisonsDisperse the config analysis
The softness of the skin2,7±0,93,4±0,8The improvement of p=3,32E-043,6±0,8The improvement of p=3,36E-04The improvement of p=2,19E-06
Surface wrinkles2,5±1,02,9±1,0NS3.3V±1,0Improving p=0.001Improving p=0.001
Wrinkles on the face2,3±1,02,9±1,0Improving p=0.012,8±0,9Improving p=0,032Improving p=0.007
Wrinkles décolleté3,2±1,43,2±1,0NS4,1±1,1Improving p=0.001Improving p=1,83E-04
SD: standard deviation; NS: not statistically significant

Table 5:
The change of skin condition - Placebo
Options(Mean ±SD)T12 (mean ±SD)T12/t0 Test tuks for multiple comparisonsT24 (mean ±SD)T24/t0 Test tuks for multiple comparisonsAnalysis of variance
The softness of the skin2,7±0,93,2±0,8NS3,2±0,9NSNS
Surface wrinkles2,9±1,13,2±1,0NS3,5±0,9NSNS
Wrinkles on the face3,0±1,13,2±1,1NS3.3V±1,3NSNS

Wrinkles décolleté4,0±0,73.3V±1,1Deterioration p=0,047 4,5±1,1NSp=0.001
SD: standard deviation; NS: not statistically significant

Dietary Supplement improves A statistically significant way, these skin manifestations as superficial and deeper wrinkles, and skin softness. Placebo does not change these parameters.

Instrumental assessment

Changes with the help of the device Torquemeter®after 6 months of dietary Supplement of a dietary Supplement or A placebo.

Table 6:
Evaluation of the biochemical properties of the skin - A Food additive
OptionsT0 (mean ±SD)T24 (mean ±SD)T24/t0 analysis of Variance
Ue (stretchability)2,91±0,5713,207±0,687Improving p=0,023
Uv (plasticity)1,894±0,4382,085±0,376Improving p=0,024
Ur (tone)1,099±0,175 1,253±0,240Improving p=0.001
SD: standard deviation

Table 7:
Evaluation of the biochemical properties of the skin - Placebo
OptionsT0 (mean ±SD)T24 (mean ±SD)T24/t0 analysis of Variance
Ue3,189±0,6953,179±to 0.645NS
(stretchability)
Uv1,960±0,3892,092±0,340NS
(plasticity)
Ur (tone)1,131±0,1671,209±has 0.168NS
SD: standard deviation; NS: not statistically significant

In the 6-month ingestion of food is obuvki A parameters related to the biomechanical properties of the skin, statistically significantly improved. These parameters were not changed when receiving a placebo.

Graphical analysis of fingerprints skin after 6 months of nutritional Supplement or A placebo.

Table 8:
Modification of skin topography - A Food additive
OptionsT0 (mean ±SD)T24 (mean ±SD)T24/t0 analysis of Variance
SRvm (the average height of the depressions)0,059±0,0090,053±0,01Improving p=0,026
Density <Z1 (density of wrinkles height ≤10 µm)of 8.06±7,18714,402±10,192Improving p=0,019
Density >Z2 (Density of wrinkles height >20 μm)73,307±14,93555,733±18,878Improving p=0.001
Anisotropy (density of wrinkles, reduced to their height and orientation)43,926±14,56134,011 the 12,114 Improving p=0.016
SD: standard deviation

Table 9:
Modification of skin topography - Placebo
OptionsT0 (mean ±SD)T24 (mean ±SD)T24/t0 analysis of Variance
SRvm (the average height of the depressions)0,059±0,0190,053±0,01NS
Density <Z1 (density of wrinkles height ≤10 µm)13,763±12,95616,76±8,729NS
Density >Z2 (Density of wrinkles height >20 μm)65,231±22,1252,168±18,494NS
Anisotropy (density of wrinkles, reduced to their height and orientation)39,117±16,25533,224±13,099NS
SD: standard deviation; NS: not statistically significant

At d the TEI skin relief is very subtle and only visible by dermatological magnifier, whereas the older he becomes visible to the naked eye. On the other hand, the skin relief is associated with the mechanical properties of the dermis. Overlapping collagen fibers deep dermis make the skin taut young, forming a dense network. This network age disarmed, skin withering away, and wrinkles become deeper. Skin topography has a special design: parallel lines intersect with the formation of rectangles, squares, trapezoids, rhombuses and triangles. Primary lines wide and a depth of from 20 to 100 microns depending on the area of skin and age. Secondary thin line.

The nutritional Supplement a for 6 months causes a statistically significant improvement in skin anisotropy, increasing the density of the secondary wrinkles (density <Z1) and the primary reduction of wrinkles (density >Z2). These parameters do not change in the placebo group. In other words, A food additive again causes the primary wrinkles, characteristic of young skin (density <Z1), reduced depth of the primary wrinkles (density >Z2), characteristic of Mature skin and reducing skin anisotropy, which usually increases with age.

Self-esteem volunteers

Quality nail was estimated by volunteers on analogue scale (0 cm - poor quality, 10 cm good quality is about). Thus, the increase in length was synonymous with improvement.

Table 10:
Changes in nails - A Food additive
T0 (mean ±SD)T6 (mean ±SD)T6/t0 Test tuks for multiple comparisonsT12 (mean ±SD)T12/t0 Test tuks for multiple comparisonsT24 (mean ±SD)T24/t0 Test tuks for multiple comparisons
Quality nail4,24±2,966,13±2,11The improvement of p=4,68E-046,48±2,16The improvement of p=2,19E-056,48±1,93The improvement of p=2,11E-05
SD: standard deviation

Table 11:
The change in the quality of nail - Placebo
T0 mean ±SD) T6 (mean ±SD)T6/t0 Test tuks for multiple comparisonsT12 (mean ±SD)T12/t0 Test tuks for multiple
comparisons
T24 (mean ±SD)T24/t0 Test tuks for multiple comparisons
Quality4,85±2,90of 5.83±2.26 andNS6,47±2,23NS6,52±2,35NS

Nail
SD: standard deviation; NS: not statistically significant

Quality nail statistically significantly improved the volunteers who food additive A. Receiving placebo did not change this parameter.

Conclusion

The combination of the data received indicates that A food additive allows to correct unwanted signs, in particular, skin, associated with the loss of imechanica indicators in particular, the skin, improving the topography and relief of the skin. Dietary Supplement promotes A restructuring of the skin from the inside, and the skin becomes more elastic and improves the structure of its surface.

1. Used in cosmetic oral by a combination of lycopene, vitamin C, vitamin E and at least one polyphenol compound derived from pine bark, where the ratio of the mass content of polyphenolic compounds to the sum of the mass concentrations of lycopene, vitamin C and vitamin E is from 0.3 to 0.7, as the sole active ingredient intended for the prevention and/or treatment of skin manifestations, selected from the group consisting of wrinkles around the eyes and corners of the mouth, wrinkles, bags and circles under the eyes.

2. The use according to claim 1, wherein the daily dose of lycopene is from 1 to 22 mg, for example from 2 to 11 mg, in particular from 4 to 7 mg.

3. The use according to claim 1, wherein the daily dose of vitamin C is from 10 to 105 mg, for example from 20 to 105 mg, in particular from 33 to 105 mg, in particular from 50 to 70 mg

4. The use according to claim 1, wherein the daily dose of vitamin E is from 1 to 17 mg, for example, from 3 to 17 mg, in particular from 6 to 17 mg, in particular from 9 to 11 mg.

5. The use according to claim 1, characterized in that the daily intake of polyphenolic compounds is about the 6 to 75 mg, for example, from 13 to 75 mg, in particular from 20 to 75 mg, in particular from 30 to 50 mg

6. The use according to any one of claims 1 to 5, characterized in that it is intended to maintain and/or restore properties such as tone, firmness, elasticity and/or density of the skin.

7. Use in cosmetics according to any one of claims 1 to 5, characterized in that it is designed to combat the cutaneous manifestations associated with weight loss, in particular, observed in a low-calorie diet.

8. The use according to any one of claims 1 to 5, characterized in that it is intended for the prevention and/or treatment of skin disorders caused by menopause.

9. The use according to any one of claims 1 to 5, characterized in that it is intended for the prevention and/or treatment of manifestations of fatigue, selected from the group consisting of eye bags and circles around the eyes, preferably for prophylaxis and/or treatment of bags under the eyes.

10. The use according to any one of claims 1 to 5, characterized in that it is intended for the prevention and/or treatment of bags under the eyes and the fact that daily dose of lycopene ranges from 2 to 11 mg, and/or daily dose of vitamin C is from 20 to 70 mg, and/or daily dose of vitamin E is from 3 to 12 mg and/or daily intake of polyphenolic compounds is from 13 to 50 mg

11. The use according to any one of claims 1 to 5, characterized in that h is on the content of phenolic trimers in polyphenolic compound, derived from pine bark, can be from 5 to 25 wt.%, preferably from 10 to 20 wt.% in relation to the total weight of polyphenolic compounds.

12. The use according to any one of claims 1 to 5. characterized in that the content of phenolic dimers in polyphenol compound derived from pine bark, is not less than 5 wt.% or may be from 5 to 25 wt.%, preferably, at least 10 wt.% or from 10 to 20 wt.% in relation to the total weight of polyphenolic compounds.

13. The use according to any one of paragraphs. 1 or 5, wherein the polyphenol compound derived from pine bark contains from 2 to 15 wt.%, for example, from 5 to 10 wt.%, phenolic acids such as ferulic acid, para-coumaric acid, caffeic acid and protomateria acid, relative to the total weight of the polyphenolic compounds.

14. Kosmeticheskaja composition for oral administration containing as the sole active ingredient combination of lycopene, vitamin C, vitamin E and at least one polyphenol compound derived from pine bark, in which the ratio of the mass content of polyphenolic compounds to the sum of the mass concentrations of lycopene, vitamin C and vitamin E is from 0.3 to 0.7.

15. The composition according to 14, in which the content of polyphenolic compounds from 0.0001 to 50 wt.%, preferably from 0.01 to 10 m is from.%, preferably from 0.5 to 2 wt.% in relation to the total weight of the composition.

16. The composition according to 14, in which the content of lycopene from 0.0001 to 50 wt.%, preferably from 0.001 to 10 wt.%, even more preferably from 0.05 to 0.2 wt.% in relation to the total weight of the composition.

17. The composition according to 14, in which the vitamin C content is from 0.0001 to 50 wt.%, preferably from 0.1 to 10 wt.%, more preferably from 3 to 6 wt.% in relation to the total weight of the composition.

18. The composition according to 14, in which the content of vitamin E is from 0.0001 to 50 wt.%, preferably from 0.1 to 10 wt.%, more preferably from 0.5 to 2 wt.% in relation to the total weight of the composition.

19. The composition according to 14, in which the content of lycopene is from 0.05 to 0.2 wt.%, the content of vitamin C is from 3 to 6 wt.%, the content of vitamin E is from 0.5 to 2 wt.%, and the content of polyphenolic compounds ranges from 0.5 to 2 wt.% in relation to the total weight of the composition.

20. Composition according to any one of PP-19, characterized in that it presents in the form of soft capsules, gelatin capsules, gels, dry or liquid emulsions, tablets, dispersible powders, vials of liquid for oral administration, or active food product.

21. Cosmetic composition for oral administration containing as the sole active ingr diente combination of lycopene, vitamin C, vitamin E and at least one polyphenol compound derived from pine bark, in which the ratio of the mass content of polyphenolic compounds to the sum of the mass concentrations of lycopene, vitamin C and vitamin E is from 0.3 to 0.7, characterized in that it presents in the form of soft capsules, gelatin capsules, gels, dry or liquid emulsions, tablets, dispersible powders or vials of liquid for oral administration.

22. Composition according to any one of p or 21, in which the polyphenol compound derived from pine bark, represents what is defined in any of § § 11-13.



 

Same patents:

FIELD: chemistry.

SUBSTANCE: invention describes a skin and hand cleaner containing the following components: a) 5-70 wt % alkyl ester and/or diester, b) 0-40 wt % surfactant, selected from a group comprising ethoxylates of fatty alcohols, sulphoesters of fatty alcohols and salts of sulphated and/or sulphonated fatty acids, c) 0.5-10 wt % thixotropy agent, such as organic bentonite, and more than 0.1 wt % hydrophilic pyrogenic silicic acid, d) 0-30 wt % of one or more abrasive agents, e) 0-5 wt % physiologically compatible carbonic ester, f) 0 to less than 10 wt % water, g) optionally one or more viscosity stabilising agents, h) optionally other cosmetic auxiliary substances.

EFFECT: cleaner removes stubborn dirt from skin and hands, has a fluidity point which enables to prevent sedimentation if there is need to use abrasive particles, is easy to make, is homogeneous and stable and is easy to store.

16 cl, 29 ex, 3 tbl

FIELD: medicine, pharmaceutics.

SUBSTANCE: invention refers to cosmetology and represents a complex cosmetic agent containing hyaluronic acid, colloidal silver, emulsion wax, water, carnosine, enfolin recovered from hydrogel of hyaluronic acid in the process of photochemical nanostructuring at wave length equal to 280 nm, while hyaluronic acid is nanostructured at a diameter of single chains up to 5 nm with the ingredients of the agent taken in certain ratio, wt %.

EFFECT: invention provides rejuvenating effect, anti-inflammatory action and lifting effect, besides it possess local immunostimulative action, extended storage period and high biological activity.

1 tbl, 3 dwg

FIELD: medicine, pharmaceutics.

SUBSTANCE: invention refers to dermatology and cosmetology, and represents a pharmaceutical composition that prevents or relieves a local dermal allergic reaction, and contains triethylene glycol, lanthanum or cerium nitrate or chloride, glycerol, polyethyl glycol-115, propylene glycol , ethanol, potassium hydroxide, cedar nut oil or milk thistle oil, vitamins A, E, C, F, analgin, glyciram, diazolin, hyaluronic acid and distilled water, wherein the ingredients are taken in a specific ratio, wt %.

EFFECT: invention provides an extended range of products improving the physiological skin condition and protecting the skin from the adverse environmental exposure.

1 tbl

FIELD: medicine.

SUBSTANCE: invention relates to medicine, namely therapeutic dentistry and may be used for treating destructive forms of chronic periodontitis. That is ensured by administering a therapeutic preparation into a root canal. A sonic phoresis of the therapeutic preparation Beresh plus drops is preceded by coating the skin surrounding a causative tooth with a therapeutic mixture containing the preparation Beresh plus drops, 10% calcium gluconate and Vaseline oil. Thereafter, an emitter is used to process the area. The sonic phoresis is followed by a root canal obturation with calcium hydroxide. That is combined with administering ascorbic acid in a daily dose of 50-100 mg and the preparation Beresh plus drops 1 drop per 2 kg of body weight a day orally. The therapeutic course is 5-8 procedures.

EFFECT: invention enables arresting the inflammatory process in the aggressive lesion, reducing the number of complications and aggravations ensured by accelerating the repair processes in the apical aggressive lesion.

2 dwg

Oral composition // 2510262

FIELD: medicine.

SUBSTANCE: group of inventions refers to medicine, namely to dentistry, and may be used as an oral composition for controlling an oral sweetener release. The above composition contains a polymer attached to the sweetener by a breakable link comprising a covalent bond, an ester bond or an amide bond. The breakable link is breakable in the oral cavity to release the sweetener. Said polymer is a polymer adhered to the mucosa and specified in methyl vinyl ether/maleic anhydride copolymer with polymer found in the amount of 0.001% to 10%. The sweetener is specified in dextrose, saccharose, maltose, dextrin, dried invert suger, mannose, xylose, ribose, fructose, lexulose, galactose, corn syrup, partially hydrolysed starch, sorbitol, mannitol, isomalt, aspartame, neotame, saccharine and its salts, sucralose, the sweeteners on the basis of dipeptides, cyclamates, dihydrochalcone with the sweetener content making 0.005% to 5%. What is also presented is a portable dispenser, a method for preparing the oral composition, a method for controlling the sweetener in the oral cavity, and using the polymer.

EFFECT: group of inventions enables the prolonged and/or more uniform sweetener release as compared to the oral compositions containing the sweeteners not attached to the polymer.

15 cl, 3 ex

FIELD: medicine, pharmaceutics.

SUBSTANCE: invention refers to pharmaceutical industry and represents an oral care composition having pH min. 8 or buffered so that to maintain the pH value min. 7, containing 3- hydroxypyridine-2-carbonyloxy-bis-(3-chlor-4-methylphenyl)borane in a combination or together with an orally acceptable carrier.

EFFECT: invention provides the stability, solubility and antimicrobial activity of borinic acid esters.

20 cl, 9 ex, 2 dwg

FIELD: medicine, pharmaceutics.

SUBSTANCE: invention refers to cosmetology and represents a composition applicable by a user as a personal hygiene product in the form of a bath or shower gel, containing: (a) no more than 11.5 wt % of an anionic surfactant representing alkyl sulphate, alkyl sarcosinate, or any combination thereof; (b) no more than 3 wt % of a zwitterionic surfactant representing fatty acid amidoalkylbetaine, (c) not less than 0.1 wt % of a hydroxyalkylcellulose derivative with a long-chain alkyl group; and (d) water.

EFFECT: invention provides preserving and improving the functionality, such as viscosity, smooth systemic action, foaming behaviour, low surfactant load, high biological degradability and low aquatoxicity of waste water flow.

22 cl, 3 tbl, 11 dwg

FIELD: medicine.

SUBSTANCE: present invention refers to cosmetology, particularly represents a hair styling composition containing a dispersion of at least one polyurethane that can be prepared by a reaction of one or more water-insoluble, water-nondispersible polyurethane pre-polymers with isocyanate functional groups with one or more compounds containing aminofunctional groups and specified in primary and/or secondary amines, and/or diamines.

EFFECT: improving the composition.

7 cl, 5 ex, 5 tbl, 2 dwg

FIELD: medicine.

SUBSTANCE: invention refers to cosmetic and pharmaceutical industry and represents a method for improving the transdermal permeability of therapeutic or cosmetic topical preparations involving the dermal administration of 1.0 to 300.0 vol. % of liquid xenon as a part of an acceptable neutral carrier or a therapeutic or cosmetic preparation.

EFFECT: invention provides the higher therapeutic or cosmetic effectiveness ensured by improving the transdermal transport of bioactive compounds.

8 cl, 13 ex, 4 dwg

FIELD: medicine.

SUBSTANCE: invention represents a method for assessing the water resistance of an antiperspirant, involving: a) sampling participants; b) fulfilling the requirement not to use any products or using the products containing no antiperspirant in the axillary region for a specific period of time; c) cleansing the axillary regions of each participant, d) applying a required amount of the antiperspirant product on one axillary region and a placebo product on the other axillary region of each participant; e) performing the stage d) until the required number of applications is completed provided more than one application is preferred; f) the last application is followed by a water test comprising a rotary motion of the participants in the swimming pool and/or swimming for a period of time of the activity in the swimming pool at a depth adequate to wet the axillary regions; g) performing a perspiration test; and h) stating if the antiperspirant shows at least the standard antiperspirant activity.

EFFECT: method improvement.

9 cl

Wound-healing drug // 2517065

FIELD: chemistry.

SUBSTANCE: invention relates to a wound-healing drug which consists of stearic acid; castor oil; zinc stearate; 70% alcohol extract of a plant collection including oak tree bark, marigold flowers, wild chamomile flowers, nettle leaves, taken in ratio of 1:3:2:1, respectively; Na salts of fatty acids of wool fat; boric acid; vaseline; glycerine; triethanolamine; treated water; vegetable oil; Tipton weed extract; wild rose oil or sea buckthorn oil; 10% propolis oily solution; natural L-amino acid-peptide biocomplex obtained by extracting native paunch manure of ruminants with 2.0-3.0% NaCl solution; purified lanolin and derivatives thereof.

EFFECT: improved bactericidal, anti-inflammatory, regenerating, antioxidant and antimicrobial properties, drug does not have allergic action.

4 ex

FIELD: medicine.

SUBSTANCE: composition contains the bactericidal substance Poviargol in the amount of 2.1-7.0 wt % and zosterin in the amount of 1.1-7.0 wt %.

EFFECT: synergetic action of the ingredients of the composition and promotion of the anti-inflammatory, radio protective, reparative action.

1 ex

FIELD: medicine.

SUBSTANCE: invention refers to using ultradisperse silver-containing systems as anti-inflammatory, anti-exudative and wound-healing agents. The ultradisperse silver-containing systems represent zero-valent silver metal nanocomposites at particle size 10-25 nm stabilised with arabinogalactine and its sulphated derivative. The invention also refers to an agent for wound and burn healing having anti-inflammatory, anti-exudative, wound-healing and antimicrobial activity, comprising said silver nanocomposites as a pharmacologically active substance and additionally containing carbomer, triethanolamine, glycerol or 1,2-propylene glycol and water. The agent is presented in the form of topical hydrophilic gel. The invention also refers to using the above agent for wound and burn healing in an individual in need thereof.

EFFECT: declared invention provides creating the hydrophilic gels of silver nanocomposites that are applicable for wound and burn healing.

3 cl, 8 dwg, 7 ex

FIELD: medicine, pharmaceutics.

SUBSTANCE: present invention refers to a topical preparation for contaminated wound healing. The above preparation contains lidocaine hydrochloride 0.1-0.2%, thrombolysin 30-36%, metronidazole 0.25-0.5%, clindamycin 0.3-0.45%, rifampicine 0.45-0.6%, olive oil 10%, sea buckthorn oil 1.5-2.0%, starch 5.1% and silver water.

EFFECT: invention promotes reducing contaminated wound complications, provides the advance of antibacterial active substances through intertissue spaces to the deep tissues with faster pathogenic flora inhibition in a wide range.

2 cl, 2 ex

FIELD: medicine, pharmaceutics.

SUBSTANCE: invention refers to pharmaceutics and medicine, namely to a soft dosage form in the form of a topical oily gel used in treating purulent skin infections and containing sodium fusidine, methyluracil (dioxomethyl tetrahydropyrimidine), an oily gel base (mineral oil and polyethylene) and additionally hydroxymethyl quinoxaline dioxide.

EFFECT: developing the preparation for treating purulent wounds, degrees 3-4 burns, decubituses and ulcers, possessing the improved antibacterial effect and regenerative action.

2 cl, 8 ex, 2 tbl

FIELD: medicine.

SUBSTANCE: invention refers to medicine and may be used for chronic wound and/or wound chamber healing. That is ensured by a multiple coating of a patient's chronic wound and/or wound chamber surface with a composition containing a culture medium conditioned by waste products and growth factors of human mesenchymal stem cells, prepared at the stages of a logarithmic and stationary growth phase of a stable cell culture of mesenchymal stem cells, containing biologically active compounds of low-molecular peptides and cytokines, used for human mesenchymal stem cell culture in vitro.

EFFECT: using the given method enables reducing chronic pyoinflammatory processes in wounds and wound chambers of various geneses in a relatively short time and ensuring a complete healing in the wounds and wound chambers of the volume up to 20 cm2.

17 cl

FIELD: medicine.

SUBSTANCE: what is presented is a group of inventions that refers to medicine, namely dermatology. The methods for improving a cosmetic wound appearance and relieving a scarring process in the skin wounds, as well as the method for activating a wound healing. The methods involve administering a pharmaceutical formulation containing pharmaceutically active relaxin into an individual having a skin wound in the amount effective for wound healing with the improved cosmetic wound appearance in comparison with the healed wound in an untreated individual.

EFFECT: group of invention provides improving the skin wound appearance, relieving the scarring process, ensured by the fact that it has been shown that relaxin reduces the angiogenesis and leads to the early disappearance of the new blood vessels that provides the total reduced blood vessels, respectively less wound redness thereby providing the better cosmetic effect.

20 cl, 15 dwg

FIELD: medicine, pharmaceutics.

SUBSTANCE: invention refers to pharmaceutical industry and represents a composition for treating wounds, ulcers, erosions, burns, freeze burns adhesions, as well as infections caused by the gram-positive and gram-negative bacteria Staphylococcus spp., Streptococcus spp., Enterococcus spp., Shigella spp., Escherichia spp., Salmonella spp., Proteus spp., Acinetobacter spp., Citrobacter spp., Pseudomonas spp., Serratia spp., Klebsiella spp., Antracoides spp., Cryptococcus spp., pathogenic fungi of the genera Microsporum, Trichophyton, Nocardia, Aspergillus, yeast-like fungi of the genus Candida (including the multiresistant strains), as well as Actinomycetes and some pathogenic protozoa (Entamoeba histolytica, Trichomonas vaginalis), containing an active ingredient in the form of 0.001-5.0 wt % of collagenase, 0.001-5.0 wt % of lysozyme and/or 0.001-5.0 wt % of sangvitirine, and an carrier in the form of 0.05-1.0 wt % of p-cyclodextrine or liposomes, and acceptable excipients.

EFFECT: invention provides stability and maintained enzymic activity for a long period of time, deep penetration into an affected skin area, potentiated complex antimicrobial and anti-adhesion action.

2 cl, 10 ex, 4 tbl

FIELD: medicine, pharmaceutics.

SUBSTANCE: invention refers to pharmaceutical composition in the form of a skin protection paste. The composition contains a film-forming agent representing poly(butyl methacrylate-co-methyl methacrylate), hydrocolloid, a filling agent, a softening agent and an alcoholic diluent in the certain proportions.

EFFECT: pharmaceutical composition has a prolonged shelf life and is able to ensure a uniform skin coverage which is characterised by the balanced characteristics of durability and flexibility.

3 cl, 2 tbl, 5 ex

FIELD: biotechnologies.

SUBSTANCE: peptides consist of four amino-acid residues that are used for stimulation of collagen production with fibroblast.

EFFECT: invention allows effective stimulation of collagenoses in fibroblast cells.

11 cl, 3 dwg, 7 tbl, 6 ex

FIELD: chemistry.

SUBSTANCE: invention relates to preparation of encapsulated protein-containing products. The method producing protein-containing products involves preparing internal contents with a protein component, followed by encapsulation of the internal contents to obtain a protein-containing product with a shell. Said internal contents have a ratio of protein to salt mixture of (1-11):(1.0-4); the salts used are soluble calcium salts, barium salts or mixtures thereof and pH corrector salts with the ratio (1.0-3.0):(1-2). The internal contents are encapsulated through a sodium alginate solution 0.5…3.0%. Also disclosed is an encapsulated protein-containing product. The product has an alginic shell based on calcium, barium or mixture thereof with the ratio of internal contents to the shell of 100:(20-50), wherein the internal contents have pH 7.5…8.5.

EFFECT: invention enables to obtain an encapsulated protein-containing product which is resistant to breakdown at low pH values and is impervious to pepsinolysis enzymes.

3 cl, 1 tbl, 6 ex

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