Magnetically controlled sorbent for endo- and exotoxin elimination in humans
SUBSTANCE: magnetite surface is modified with a compound forming a stable bond to a carrier particle by using surface-active groups giving the selectivity properties, and presented in the form of a coating of normal hydrocarbon chains C12H25 attached to a nucleus by a sulphide bond Fe-S; the above compound providing the bond of iron to the hydrocarbon chain is dodecyl mercaptan. Magnetite is prepared of ferric chloride (II) and ferric chloride (III) by chemical deposition with the concentration of 1 wt % in the solution and a mole ratio of iron (II) and (III) = 2.5, or of ferric sulphate FeSO4 (II) with adding a mixture of aqueous solution of potassium nitrate KNO3 and potassium hydroxide KOH at 3 moles of KOH and 0.1 mole of KNO3 per 1 mole of FeSO4.
EFFECT: invention provides the sorbent that is used to achieve the effective control of hazardous ingredients of blood plasma.
2 cl, 2 tbl
The invention relates to medicine and can be used in the treatment of endotoxemia, which accompany most urgent surgical diseases. According to most researchers, under the endotoxicosis (endogenous intoxication) refers to the manifestation of various pathological conditions, which is the basis of effects on the body of toxic products formed therein. The development of endotoxemia begins with receipt of toxins from the primary site of toxicity in the blood, lymph, interstitial space and spreading the blood around the body. If the primary source of endogenous intoxication cannot be removed surgically, and the protective system of the body is not able to neutralize the increasing flow of toxins, then there is a further progression of endotoxemia. In this case, only the additional, faster by removing toxins from the body able to resolve the critical situation. One such method is extracorporeal detoxification using filtration and sorption technologies. In this invention it is proposed to use magnetic sorbents in the form of suspensions of nano - and micro-size particles particles coated with a special molecular shells DL the biocompatibility and implementation of target sorption of toxins on the surface of the particles.
The known method of hemosorption on carbon sorbents for the treatment of diseases of the hepatobiliary system in which carbon hemosorbent has on its surface the volume of mesopores 0,17-0,35 cm3/g, micropore volume of 0.03 to 0.05 cm3/g and macropores 0.06 to 0.10 cm3/g when the total volume of all pores 0,26-0,50 cm3/g, and contains oxygen functional groups in the amount of 0.04-0.05 mEq./g .
The disadvantage of this technical solution is the use of sorbent, non-magnetic properties, which does not allow to achieve high rates of sorption efficiency in relation to remove toxic ingredients from the plasma of the human body.
Known, in addition, the magnetic sorbent prepared on the basis of micro - and nanoparticles of magnetite coated with polymerized silicic acid containing specific ligands .
However, this sorbent is intended solely for the adsorption of nucleic acids and can not provide efficient removal of endo - and exotoxins from the human body, such as total cholesterol, urea, creatinine and other toxins.
The applicant has set itself the practical task of developing the magnetic sorbent for removal of endo - and exotoxins from the human body, which is achieved effective control over the OC is ω harmful ingredients plasma, requiring mandatory output from the human body. A positive result was achieved due to a new essential features of the claimed magnetic sorbent for removal of endo - and exotoxins from the human body, is recorded in the following claims: "the magnetic sorbent for removal of endo - and exotoxins from the human body, made from nanoparticles of magnetite Fe3O4obtained on the basis of the chlorides of bivalent iron (II) and ferric chloride (III) or derived from ferrous sulfate FeSO4(II) adding the mixture of aqueous solutions of potassium nitrate KNO3and potassium hydroxide KOH, the surface of magnetite modified bifunctional compounds, forming a strong bond with the particle carrier through the surface-active groups, giving properties of selectivity and executed as a shell of normal hydrocarbon chains C12H25attached to the core by sulfide Fe-S, and the binding of iron with carbon chain carried out by a mercapto-group SH-R; the size of nanoparticles of magnetite Fe3O480÷150 nm; the magnetite nanoparticles of Fe3O4on the basis of ferric sulfate FeSO4(II) is added a mixture of aq is x solutions of potassium hydroxide KOH and aqueous solutions of potassium nitrate KNO 3taken at the rate of 3 mol of KOH and 0.1 mol KNO31 mol FeSO4(II); the bearer of the mercapto-group SH-R is dodecylmercaptan, providing the binding of iron with carbon chain; 1 g of a suspension of nanoparticles of magnetite in 50 ml of diphenyl ether is added 0.2 g of dodecylmercaptan".
Implementation of the invention is as follows.
Synthesis of magnetic carriers is carried out in two ways. Method No. 1 was based on the method of chemical deposition of salts of bivalent and trivalent iron. Why in the reactor is placed aqueous solutions of chlorides of iron (II) and (III) their concentration in solution of 1% of the mass. and the molar ratio of iron (II) and (III) = 2,5, which is freed from dissolved oxygen by passing nitrogen for 20 minutes.
Synthesis of magnetite is carried out by adding 8% of the mass. hydrate of ammonia dropwise to the reaction solution at room temperature and vigorous stirring. For complete formation of nanoparticles of magnetite Fe3O4the slurry is mixed for another 20÷40 minutes. The obtained magnetic dispersion is washed by centrifugation or sedimentation on the magnet with repeated washing with distilled water to lower the pH of the wash water is ~7÷8. The size of nanoparticles of magnetite Fe3O480÷150 nm.
Method 2 is based on Sul the ATA iron FeSO 4(II). When this aqueous solution of ferric sulfate FeSO4(II) is heated to 90°C in argon atmosphere. Then add drops with vigorous stirring a mixture of aqueous solutions of potassium nitrate KNO3and potassium hydroxide KOH at the rate of 3 mol of KOH and 0.1 mol KNO31 mol of ferrous sulfate FeSO4(II). Suspension with a black precipitate leave much to be mixed at the temperature of the reaction mixture to 90°C in argon atmosphere for two hours to complete crystallization of the particles of ferric sulfate FeSO4(II). The obtained magnetite was washed with water, then isopropyl alcohol and dried in vacuum at room temperature.
To make the surface of the particle extraction properties when using the magnetite nanoparticles obtained by methods 1 and 2, form the shell of a normal hydrocarbon chains With12H25attached to the core by sulfide Fe-S, and the binding of iron with carbon chain is realized by means of mercapto-group SH-R. then prepare a suspension of 1 g of the magnetite nanoparticles in 50 ml of diphenyl ether, treating the mixture with ultrasound for 30 minutes. In the resulting mass is added 0.2 g of dodecylmercaptan and boil at a temperature of 260°C for one hour with stirring. At the end of the process the product is separated by centrifugation and washed with ethanol and or what propanolol (number of experiments, respectively, D-1 and D-2).
The study of sorption properties of nanosorbent were performed on blood plasma and lymph for 12 people (donors). Bioliquids were given the 3rd Central military clinical hospital named A.A. Vishnevsky (3 CVKG them. A.A. Vishnevsky). As controlled criteria values of sorption capacity used indicators of content in blood plasma and lymph of their basic regular ingredients, the concentration of which you can judge the state of the cell, organ and overall homeostasis in various diseases.
To study the sorption was preparing a suspension of the modified magnetite in physiological solution with a concentration of 12.0±3.0 mg/ml, which were treated by ultrasound for 40 minutes in an ultrasonic bath Elmasonic-S-100 H at a frequency of 37 kHz for disaggregation of the particles and their distribution within the volume. Then mixed with 0.5 ml each of the resulting suspension with 3 ml of blood plasma. The resulting mixture was kept under constant shaking for 3 minutes and then separated particles by centrifugation at 2500÷3000 rpm for 15 minutes.
Controlled performance obtained with this technology the magnetic sorbent was following parameters.
ALT (alanine aminotransferase) and AST (aspartate aminotransferase) - specialized proteins (enzymes), which contains NR the three cells of the body and are involved in the metabolism of amino acids (substances, of which are proteins). Chol (total cholesterol) - source education in mammals bile acids, corticosteroids, sex hormones, vitamin D3. UREA (urea) - the major end product of nitrogen metabolism, quantitative determination of blood and urine is important for the diagnosis of pathological conditions of the liver and kidneys. Crea (creatinine) - the end product of protein metabolism, which is formed in the muscles and then is released into the blood. BiliT (total bilirubin) is the result of the breakdown of hemoglobin, myoglobin and cytochromes in the cells of the reticuloendothelial system, spleen and liver. GluC (glucose) - the main indicator of carbohydrate metabolism. TP (total protein) is the most important component of protein metabolism in the body, characterized by the total concentration of albumin and globulin in the blood serum. AlbG (albumin) is the most representative part of blood plasma proteins, plays a significant role in maintaining colloid osmotic pressure in the blood and is important for the body reserve of amino acids.
Analysis nasadochnykh bioliquids were conducted in the biochemical laboratory 3 CVKG them. A.A. Vishnevsky. Content controlled ingredients in the blood before and after adsorption was determined at the laboratory of biochemical analyzers Olympus (Germany) Advia 1200 (USA - Germany) and the system wedge is ical capillary electrophoresis Paragon (USA).
|The sorption efficiency of the modified magnetite in relation to the ingredients of the blood plasma (averaged relative to the initial concentration)|
|Ingredients plasma||Experience D-1||Experience D-2|
|The sorption efficiency of the modified magnetite in relation to the ingredients of the blood plasma (averaged relative to the initial concentration)|
|Ingredients plasma||Experience D-1||Experience D-2|
Sources of information
1. Description of the invention to the patent of the Russian Federation "Method of hemosorption on carbon sorbents for the treatment of diseases of the hepatobiliary system No. 2343926, CL A61K 33/44, A61P 1/16, declared 10.09.2007, published 20.01.2009.
2. U.S. patent No. 8206990, class G01N 30/56, published 2012p> 3. Description of the invention to the patent of the Russian Federation "Method of treatment of mesoporous carbon hemosorbent" No. 2362733, CL C01B 31/08, B01J 20/20, declared 19.05.2008, published 27.07.2009.
4. U.S. patent No. 2007/0105094 A1, class C72Q 1/70 (435/5), published 10.05.2007,
5. Description of the invention to the patent of the Russian Federation "Magnetic sorbent and method thereof" No. 2255800, CL A61M 1/36, declared 19.05.2008, published 27.07.2009.
6. Description of the invention to the patent of the Russian Federation "Magnetic sorbent, the manufacturing method and the method of its application" No. 2356620, CL B01J 20/06 declared 23.04.2008 published 27.05.2009.
7. Description of the invention to the patent of the Russian Federation "Porous magnetic sorbent No. 2226126, CL B01J 20/16 declared 30.12.2002, published 27.03.2004.
8. Description of the invention to the patent of the Russian Federation "Porous magnetic sorbent No. 2241537, CL B01J 20/26, declared 09.04.2003 published 10.12.2004.
1. The magnetic sorbent for removal of endo - and exotoxins from the human body, made from nanoparticles of magnetite Fe3O4received iron chloride (II) and iron chloride (III) by chemical vapor deposition with their concentration in solution of 1% of the mass. and the molar ratio of iron (II) and (III) = 2.5 or iron sulfate FeSO4(II) adding the mixture of aqueous solutions of potassium nitrate KNO3and potassium hydroxide KOH at the rate of 3 mol KOH and 0.1 mol KNO31 mol FeSO4while the surface of the magnetite modifica avana connection forming a strong bond with the particle carrier through the surface-active groups, giving properties of selectivity and executed as a shell of normal hydrocarbon chains C12H25attached to the core by sulfide Fe-S, and as mentioned compounds, providing the binding of iron with carbon chain selected dodecylmercaptan.
2. The sorbent according to claim 1, in which the size of the nanoparticles of magnetite Fe3O4is 80-150 nm.
SUBSTANCE: invention relates to processing of drinking water with application of sorption purification. Method of final purification of drinking water includes mechanical filtration of water through wooden activated carbon sorption charge and introduction of sodium hypochlorite into initial filtered water. Filtration of initial water is carried out at specified rate, corresponding to time of contact of filtered water with sorption charge for 8-12 minutes. Index of oxidisability in filtrate is periodically measured. If index of oxidisability in filtrate increases by 25-30%, specified rate of filtration is reduced by 2-3 fold. Sodium hypochlorite is introduced into initial filtered water with concentration 60-80 mg/l, after which sorption charge is washed with reverse flow of purified water.
EFFECT: invention makes it possible to increase sorption capacity of carbon in the process of final processing of water.
2 cl, 1 dwg, 1 tbl
SUBSTANCE: invention relates to field of obtaining chemosorbents, which are applied for means of protection of respiratory organs and for purification of discharge gases. Method of obtaining chemosorbent includes impregnation of activated coal granules with modifying solution, maturing granules and their thermal processing. Active coal with volume of micropores 0.36-0.55 cm3/g is subjected to impregnation. Impregnation solution contains copper sulfate, water and sulfuric acid in ratio 1:(8-10):(0.3-0.5). Coefficient of impregnation constitutes 0.8-1.0.
EFFECT: invention makes it possible to increase duration of protective action of obtained chemosorbent by benzene with preservation of high adsorption activity.
2 cl, 3 ex
SUBSTANCE: invention relates to method of obtaining active coal. Method includes separation of 1-3 mm fraction from charge for layered high-temperature coking and the following stages: oxidation with air oxygen at temperature 250°C with rate of temperature increase from room to specified temperature 15-20°C/min with keeping at final temperature for 2.5 hours, carbonisation of oxidised raw material with temperature increase with rate 5°C/min to temperature of carbonisation 550-650°C, with keeping at temperature of carbonisation for 60 minutes, and activation of obtained products with water steam to 35-40% burn at temperature 950°C.
EFFECT: providing possibility of obtaining active coals from charges of coking production without introduction of any additional components.
SUBSTANCE: invention can be applied for purification of technological sewages of chemical industry enterprises. Method of purifying water solutions from pyridine by adsorption by activated coal includes processing of activated coal with ammonium chloride with concentration 5 mg/dm3 for 3 hours. Ratio of activated coal weight to volume of ammonium chloride constitutes 1:100. Pyridine adsorption from its water solution is carried out in static conditions.
EFFECT: invention makes it possible to increase efficiency and economy of purification process.
3 tbl, 15 ex
SUBSTANCE: coal is obtained by carbonisation and following activation of polymeric organic, sulfonated initial substances. Installation includes means of drying for drying initial substances, means of sulfonating, placed downstream relative to means of drying. Further, installation contains means of carbonisation, placed downstream with respect to means of sulfonating, as well as means of activation, placed downstream with respect to means of carbonisation, where activation of carbonised products is performed. Installation also includes means of processing discharged gases, which are formed in means of carbonisation and/or in means of activation in the course of process. All discharged gases are subjected to afterburning.
EFFECT: increased efficiency of the process and its ecological safety.
6 cl, 1 dwg
SUBSTANCE: invention relates to technological processes of obtaining wood-based active coal. Method of obtaining activated coal lies in drying, carbonisation and activation of raw material in reactor and in further discharge of obtained product. As raw material used is wood chips with particle size from 1 mm to 10 mm and initial humidity from 30% to 70%. Drying, carbonisation and activation are carried out with straight flow movement of chips through reactor with rate of temperature rise in it 20-80°C/min to pyrolysis temperature 800-900°C, at which air is supplied into reactor.
EFFECT: invention ensures obtaining coal with high sorption iodine activity.
2 cl, 9 ex
SUBSTANCE: invention relates to carbon materials. Described is a method of producing porous carbon material from silicon-containing plant material. The method involves carbonising plant material by temperature treatment, treating with acid or alkali, followed by activation. The porous carbon material has nitrogen BET specific surface area of at least 130 m2/g, mesopore and micropore volume of at least 0.1 cm3/g, measured by BJH method and MP method and containing more mesopores smaller than 20 nm than mesopores having size of at least 20 nm. The obtained material is used, for example, as an adsorbent, an adsorbent for oral administration, a medical adsorbent, a creatinine adsorbent, a cap for blood purification columns, a water treatment adsorbent, a mask, an adsorbent sheet, a medicine carrier and a carbon-polymer complex.
EFFECT: invention enables to obtain material with a wide range of applications.
10 cl, 10 dwg, 12 tbl, 8 ex
SUBSTANCE: invention relates to porous carbon materials. Disclosed is a material obtained by reducing silicon content in plant material, such as sugar cane, seaweed stems, rice husks and straw. The method of producing porous carbon material from silicon-containing plant material involves carbonising plant material during high-temperature treatment, treating with acid or alkali, followed by activation. The disclosed carbon material has nitrogen BET specific surface area of at least 10 m2/g, mesopore volume of at least 2.2 cm3/g, measured by mercury press-in method, mesopore volume of at least 0.1 cm3/g, measured by BJH method and MP method. The obtained carbon material can be used, for example, as a medical adsorbent, a creatinine adsorbent, a cap for blood purification columns, a water treatment adsorbent, an adsorbent sheet, a medicine carrier and a carbon-polymer complex.
EFFECT: invention enables to obtain material with high functionality.
10 cl, 10 dwg, 12 tbl, 8 ex
FIELD: medicine, pharmaceutics.
SUBSTANCE: group of inventions refers to a method for preparing a carbon sorbent with the antibacterial properties, and to the carbon sorbent with the antibacterial properties prepared by this method. The declared method involves impregnation of the carbon hemosorbent granules in an initiator solution in N-vinylpyrrolidone at pH 7.0-7.5 and a residual pressure of 15-20 mm Hg. The hemosorbent : initiator solution in N-vinylpyrrolidone ratio is 1:1.4-2.0. Then the temperature is raised to 65-75°C, kept at that temperature for 0.5-8 hours in an inert medium and washed in water from the residual monomer at room temperature.
EFFECT: carbon sorbent with the antibacterial properties prepared by the specified method represents the round granules, contains polyvinylpyrrolidone in an amount of 4,5-5,5% and is characterised by a specific surface adsorption of less than 50 m2/g and total pore volume of less than 0,30 cm3/g.
2 cl, 2 tbl, 1 dwg, 7 ex
SUBSTANCE: invention relates to production of activated carbon. Disclosed is a method of forming activated carbon from microcrystalline cellulose, involving: converting microcrystalline cellulose powder into microcrystalline cellulose granules by mixing microcrystalline cellulose powder with water and extrusion to obtain spherical granules. The method involves carbonisation the microcrystalline cellulose to obtain carbonised microcrystalline cellulose and activation of the carbonised microcrystalline cellulose to obtain activated carbon. Disclosed also is a method of making a smoking article filter which contains the obtained activated carbon granules, and a method of making a smoking article having said filter.
EFFECT: invention provides an optimum porous structure of activated carbon used in smoking article filters.
9 cl, 2 dwg
SUBSTANCE: diamond-like coatings are produced in vacuum by spraying of target material with an impulse laser. The target material made of graphite of high degree of purity (more than 99.9%) is exposed to combined laser radiation: first short-wave (less than 300 nm) pulse radiation, the source of which is a KrF-laser with wavelength of 248 nm and specific energy of 5·107 W/cm2, as a result of which ablation is carried out, and gas-plasma phase of target material is generated. Subsequent exposure of a gas-plasma cloud during cloud flight from a target to a substrate is carried out by long-wave (more than 1 mcm) laser radiation. The source of long-wave laser radiation is a gas CO2-laser or a solid-state fibrous laser radiator.
EFFECT: increased diamond phase in a produced coating and increased energy spectrum of plasma at stage of its flight.
3 cl, 1 dwg
SUBSTANCE: invention relates to chemical industry. Carbon-metal material in form of mixture of carbon fibres and capsulated in non-structured carbon nickel particles with diameter from 10 to 150 nanometers are obtained by catalytic pyrolysis of ethanol at atmospheric pressure. Catalyst in form of nickel and magnesium oxides, applied on the surface of graphite foil as inert substrate in dust-like or granulated state, is placed into closed hermetic capacity, in which constant temperature 600 - 750 °C is supported. Ethanol vapour is supplied through input collector, and gaseous pyrolysis products are discharged through output collector. Ethanol vapour is diluted with inert gas, for instance, argon, with weight ratio ethanol: inert gas 1:4…5. Time of synthesis is from 1 to 180 min.
EFFECT: invention makes it possible to obtain carbon nanomaterials from renewable raw material and simplify the process.
6 cl, 3 dwg, 2 ex
SUBSTANCE: invention relates to chemical and electrotechnical industry and can be applied for modification of rubbers and caoutchoucs, in production of high-capacity condensers and composite materials. Finely milled mixture, containing, wt.p.: carbon - 100, oxidiser - 200, is placed into heat-resistant vessel, closed with heat-resistant mesh cover, and heated in furnace to 150-250°C. Pyrolysis of mixture is carried out in mode of self-propagating high-temperature synthesis with heating to 900°C at rate 20-30°C/min, with keeping at said temperature for 15-30 minutes and discharge of excess of released gas. As carbon, used is sugar, glucose, fructose or starch, with ammonium nitrate being used as oxidiser. Nanostructured carbon foam with volume density 0.006 g/cm3 and specific surface not less than 150 m2/g is obtained. Output of carbon foam constitutes 31% of carbon weight.
EFFECT: release of aggressive products is excluded.
7 dwg, 4 ex
SUBSTANCE: invention can be used in obtaining composite materials. Initial carbon nanomaterials, for instance, nanotubes, nanothread or nanofibres, are processed in mixture of nitric and hydrochloric acid at temperature 50-100°C for not less 20 min, washed with water and dried. After that, it is empregnated with alcohol solution of oligoorganohydride siloxane, for instance, oligoethylhydride siloxane or oligomethylhydride siloxane, evaporated, air-dried at temperature not higher than 200°C for not less than 20 min, then tempered in inert medium at temperature 600-800°C for not less than 20 min.
EFFECT: obtained carbon nanomaterials with applied silicon dioxide have high resistance to oxidation.
2 cl, 4 dwg, 6 ex
SUBSTANCE: heating, evaporation and deposition of aluminium oxide on the metal substrate with a particular crystal orientation is carried out. Deposition of vaporised stream consisting of AlO particles and (AlO)2 is carried out. The vaporised stream consists of AlO particles and (AlO)2, and after each subsequent monolayer the exposure is carried out in molecular oxygen at a partial pressure of 10-7 mmHg for 3 minutes at a substrate temperature of 700°C.
EFFECT: oriented highly stable nanoscale film is obtained on a clean surface of metal-substrate while maintaining the interphase oxide-metal border at the atomic level.
SUBSTANCE: invention relates to the field of physical and colloidal chemistry and can be used in obtaining polymer compositions. Finely-disperse organic suspension of carbon metal-containing nanostructures is obtained by interaction of nanostructures and polyethylene polyamine. First, powder of carbon metal-containing nanostructures, representing nanoparticles of 3d-metal, such as copper, or cobalt, or nickel, stabilised in carbon nanostructures, are mechanically crushed, after which, mechanically ground together with introduced in portions polyethylene polyamine until content of nanostructures not higher than 1 g/ml is reached.
EFFECT: invention ensures reduction of energy consumption due to the fact that obtained finely-disperse organic suspension of carbon metal-containing nanostructures is capable of recovery as a result of simple mixing.
2 cl, 5 dwg, 2 ex
FIELD: measurement equipment.
SUBSTANCE: device is designed for performance of probing measurements on objects of complex shape, for instance, on pipes in oil and nuclear power industries. The substance of the invention consists in the fact that into a scanning probe microscope for investigation of bulk objects, comprising a measurement head with a piezoscanner and a probe, coupled with a unit of analysis and control, an approach module, three support stands installed on the measurement head, and a drive of the measurement head, included into the approach module, additionally a platform is added, on which there is a double-coordinate table installed, being coupled with the body installed on it as capable of rotation, on which there is the approach module, in which the measurement head is fixed with the piezoscanner and the probe. The measurement head comprises two spring supports, and the platform - modules of fixation to the object.
EFFECT: expansion of functional capabilities of a device.
7 cl, 2 dwg
FIELD: machine building.
SUBSTANCE: prior to applying coating onto the article surface it is necessary to evacuate air from the vacuum chamber, clean the article surface and the vacuum chamber in the inert gas environment, perform ion etching and ion-plazma nitriding of the article surface. The coating is produced by applying a microlayer of nanolayers of titanium and aluminium 1-100 nm thick and a microlayer of nanolayers of titanium nitride and aluminium nitride 1-100 nm thick. After each of the microlayers is applied, the ion-beam cleaning of the surface by argon is carried out for 10 minutes under pressure of 1.5 Pa and displacement voltage of 1150 V. Application of microlayers and the following ion-beam cleaning is performed in N stages, where N is an integer number and N ≥ 1, up to the production of a protective coating with the total thickness of 5.8-7.2 and more.
EFFECT: extended service life of the coating under erosion, corrosion and high temperatures.
4 cl, 1 tbl
SUBSTANCE: invention relates to medicine and specifically to trauma surgery and orthopaedics, and can be sued for surgical treatment of ununited fractures and false joints of cylindrical bones when there is a shortage of soft tissue. The method involves, 5-6 days before an operation, performing needle biopsy of bone and soft tissue fragments from the damage centre of the cylindrical bone and determining presence and nature of obligate intracellular viral infection (OIVI). Super-selective angiographic analysis of the microvascular channel to the capillary link is also performed. Valtrex is administered to the patient 2-4 days before the operation in a dose of 500 mg twice a day. Further, the method involves performing osteosynthesis or re-osteosynthesis with resection of the ends of bone fragments, opening marrowy canals, bone stimulation and batting the space of the bone defect with a gel-like nanostructured composite implant. In the presence of OIVI, resection of bone fragments is carried out in a larger volume until the onset of "pinpoint bleeding", i.e. to areas with satisfactory intrabone blood supply. The composite implant contains thrombocyte-rich autoplasma, mixed in ratio of 1:(1-2) with granules of a complex alloplastic preparation (CAP) based on hydroxyapatite which contains 50-60 wt % collagen. The composite implant also contains either 0.08-2.8 wt % colloidal solution of nanoparticles of zero-valent silver metal Ag0, or gold Au0, or copper Cu0, or palladium Pd0, or platinum Pt0, or 5-12 wt % nanoparticles of said metals in dry form. The nanoparticles have size of 2-40 nm. A colloidal solution of said nanoparticles or colloidal nanoparticles of said metals in dry form is added to the CAP granules. Further, the prepared granules of the gel-like complex alloplastic preparation are laid in a selected ratio on the layer of thrombocyte-rich autoplasma, without mixing, for subsequent transfer into the bone defect space. In case of performing resection of bone fragments in a larger volume until the onset of "pinpoint bleeding", corticotomy is further performed on the cylindrical bone being operated on, with subsequent distraction of the bone regenerate using any existing method. Further, the bone fragments are repositioned, followed by metallo-osteosynthesis. Before wound suturing, the surface of the area with shortage of soft tissue in the projection of the ununited fracture and false joints is covered by a semi-permeable flexible plate made of the complex alloplastic preparation based on hydroxyapatite, which contains 50-60 wt % collagen. The plate has thickness of 0.25-1.2 mm. The surface area of the plate is 10-20% greater than the area with shortage of soft tissue in the corresponding projection. The part of the erythrocyte mass remaining from preparing the thrombocyte-rich autoplasma and the plasma are returned into the bloodstream of the patient by intravenously using a drip during the surgical procedure or in the early post-operation period. After the operation, valtrex is administered to the patient in a dose of 500 gm once a day for two weeks and then in a dose of 500 mg every other day for two weeks.
EFFECT: method provides reliable prevention of OIVI at a damage centre, normalisation of local microcirculation of blood, avoiding ischemic processes, and compensation for the shortening of the length of the limb of the patient being operated on while preventing weakening of the process of reparative osteogenesis and allergic reactions of the body.
6 cl, 4 ex
SUBSTANCE: proposed method comprises mixing of nickel compounds and poluolyc alcohol, heating of obtained mix, cooling it and centrifugation, flushing and drying of produced precipitate. Nickel compounds and poluolyc alcohol are taken at the ratio of 1-2 mmol/mol. Additionally alkali is introduced at the ratio of 2-3 mol to nickel mol and surfactant in amount of 10-30 mmol to nickel mol. Mix is heated in stepwise manner: First, it is heated from room temperature to 120°C at the rate of 10°C/min, then, from 120 to 190°C at the rate of 3.5°C/min. Thereafter, said mix is held for at least 10 minutes and cooled with the help of frozen distilled water. Multicomponent suspension after cooling is subjected to centrifugation, flushed and dried in air.
EFFECT: higher catalytic activity and safety, non-polluting process.
8 cl, 3 dwg, 22 ex
SUBSTANCE: formed sorbent comprises a nanodispersed mesoporous carbon material in the form of cylinders of the diameter of 8-13 mm, the length of 50-80 mm, the thickness of an external wall of 2.2-3.0 mm with one round inner passage or six triangular passages with the thickness of passage partition of 1.1-1.2 mm. The above sorbent contains at least 99.5 wt % of carbon, no more than 0.15 wt % of ash, no more than 0.30 wt % of sulphur; it has the total porosity not less than 0.4 cm3/g, the specific BET absorption surface of 200-300 m2/g and the crushing strength not less than 50 kg/cm2. The group of inventions also refers to a method for making the above sorbent involving mixing of nanodispersed carbon with 0.2-3% aqueous polyethylene oxide in ratio 1.0-1.2 respectively, extrusion of the mixture, drying of the extrudate, keeping at 200°C in an inert medium, thermal treatment in the hydrocarbon medium and activation with water steam at 700-950°C, and further pneumohydromechamnical treatment and drying. What is also declared is a method for prevention of suppurative septic complications in obstetric patients with a risk of an infection, involving the uterine cavity sanitation by placing the above sorbent therein in a capron container.
EFFECT: group of inventions enables reducing a risk of developing endometrium and reducing the number of antibiotics used in the obstetric patients.
3 cl, 2 tbl, 10 ex