FIELD: chemistry.

SUBSTANCE: invention discloses a disinfectant and can be used in prevention and treatment facilities, at first aid facilities, disinfection facilities, child care centres, public catering facilities, public utilities, transportation, veterinary, agriculture, food industry enterprises and other sectors, as well as for pre-sterilisation cleaning of medical items, including surgical and dental instruments, rigid and flexible endoscopes and other instruments. The disinfectant contains catamine AB, isobornyl acetate, isopropanol, α-pinene, glutaric aldehyde, glycerine, a nonionic surfactant and water in defined ratios.

EFFECT: disinfectant does not cause corrosion in articles made of metal and decomposition of metals and endoscope units; does not cause organic contamination.

3 tbl


The invention relates to disinfecting with a bactericidal and fungicidal effect and surface-active properties and can be used for handling objects contaminated by conditionally pathogenic microorganisms.

Known disinfectants containing terpene alcohols and hydrocarbons or essential oils of plants.

Similar to the proposed disinfectant is a disinfectant celades containing pine oil with a share of alcohols of at least 70%, surfactant (sodium salt of a mixture of tall oil fatty acids) and water [1]. It has a pronounced antimicrobial action against bacteria groups, coliforms and staphylococci, surface-active properties and deodorizing effect.

Subsequent developments aimed at expanding the range of active action of disinfectants.

The closest in composition to the proposed the sanitizer air disinfection is the "Receptek" (Patent of the Russian Federation 2197994, 25.05.2000, CL. A61L 2/16), (2)prototype containing pine oil, katamin AB, isobutylacetate, ethoxylated fatty alcohols and/or alkyl phenols (SAS), ethanol or isopropanol and water in the following ratio of components, wt.%:

pine oil with a share of terpene alcohols of not less than 70% - 1,0-15,0

katamin AB - 5,0-20,0

isobutylacetate - 1,0-15,0

ethoxylated fatty alcohols and/or alkyl phenols - 10,0-20,0

ethanol or isopropanol - 5,0-10,0

water - the rest

Disinfection means lisatek on the basis of natural terpenoids has antimicrobial and fungicidal action of surface-active properties. Fungicidal effect of the drug "Receptek" seen in sufficiently high for the disinfectant concentration of the working solution of 5 wt.%. Bactericidal effect of the drug "Receptek" cover gram-negative and gram-positive bacteria and microbacteria, not including crops such as Pseudomonas aeruginosa, Escherichia coli, Staphylococcus aureus, Klebsiella oxytoca, i.e. the range of its validity can be extended.

The technical result of the proposed solutions expanding the range of active bactericidal (including bacteria: Pseudomonas aeruginosa, Escherichia coli, Staphylococcus aureus, Klebsiella oxytoca) and fungicidal action, lowering the concentration of the working solution.

This technical result is achieved in that the disinfectant containing katamin AB, isobutylacetate, isopropanol, surfactant and water, additionally contains a-pinene, glutaric aldehyde, glycerin, and as surface-active substances sintimid-5K in the following ratio of components, wt.%:

kata is in AB - 15-25

α-pinene - 7-10

isobutylacetate - 7-10

glutaric aldehyde - 5-7

glycerin - 1-5

isopropanol - 1-10

sintimid-5K - 1-2

water - the rest.

Katamin AB (alkyldimethylbenzylammonium chloride) is a Quaternary ammonium compound (QAC). The main elements of the structure, which determine the antimicrobial properties of the HOUR, include hydrophilic and hydrophobic hydrocarbon radicals. Also antimicrobial properties of H are determined by their surface-active properties. The mechanism of action HOUR, as a surfactant on living cells, is a change in cell permeability by interacting with lipid-protein membranes of living entities. In the process of penetration HOUR in the cage is the denaturation of cell proteins, disruption of the enzyme balance in the body. The HOURS applied for disinfection of surfaces, clothes, dishes and other objects infections (excluding TB). THE 9392-098-56856807-2011 production "Bursitis-M".

It is known that microbes in prolonged contact with natural oils not actually produce them stability. Pine oil is a complex mixture of several hundred components, the activity of which is very difficult to judge. We applied the disinfectant composition of α-pinene - terpenoid, the main component of skiped is a (pine oil), used as an independent substance, which allowed to evaluate its activity, to compare with the fungicidal activity of pine oil, α-pinene destructive acts on the cytoplasmic membrane of microorganisms, reducing their permeability, reducing the activity of aerobic respiration of microbes, i.e. observed and antibiotic effect through modification of cherishing environment [3-5]. In the manufacture of the drug, "Roderick" used α-pinene cleared of production of JSC "Balm" on THE other 2416-024-05788576-96.

Isobutylacetate - terpenoid, ester isoborneol and acetic acid, is found in many essential oils. The mechanism of action like essential oils.

Glutaric aldehyde C3H5O2- organic substance having sterilizing and disinfecting properties. The main advantage of glutaraldehyde is a gentle effect on the interest and activity in relation to all types and forms of microorganisms for the alkylation of amino, carboxyl and sulfhydryl groups of proteins and other organic compounds, and suppression of the synthesis of the latter. Glutaric aldehyde reacts with the amino group of the cell walls with the formation of the amine bridge connection. In the acidic environment of Glutaric aldehyde penetrates into the cell in an alkaline environment (pH 7.5 to 8.5) reaction takes place with NR is Snimi layers of cells, stopping their division. When using glutaraldehyde observed effect bonding of the cells, which is manifested in increased speed decanting cells. In addition, in sanitizer parowy aldehyde acts as an anti-corrosion additive that gives the opportunity to use the tool for handling medical instruments and medical equipment.

Glycerin is a trivalent alcohol With3H5(OH)3(1, 2, 3-trihydroxypropane). Treatment of alcohol antiseptics (with ethanol) provides the highest level of purity. However, frequent use of ethanol for disinfection of rubber products (gloves, endoscopes) leads to the appearance of microcracks. In addition, the use of ethanol contributes to the fixation of organic pollution, the rapid decrease in concentration due to evaporation. For this reason, technologically preferable to use glycerin. In addition, the glycerol has a beneficial effect on the skin.

Isopropanol is used as solvent terpene compounds (LLC Synthesization" according to GOST 9805-84).

As the surfactant used, the preparation of nonionic sintimid-5K on THE 2483-064-05807977-2003) produced by LLC "Sintez OKA". The surfactant improves the detergency of the composition as a whole, and also allows jet to increase the permeability of the active ingredients of the drug.

The disinfectant is obtained by thorough mechanical mixing all of the components in its composition. The composition of the technically reproduced with the boundary and the averaged values of the initial components.

Below is an example of obtaining a disinfectant "Roderick".

In a glass reactor with a capacity of 20 kg load 1 kg of α-pinene, 1 kg of isobutylacetate. The reactor is closed and carefully mix until dissolved. Next, under stirring add 1 kg of isopropanol, 0.2 kg of sintimid 5K, 0.1 kg of glycerol. The mixture was thoroughly stirred for 5-10 minutes. Then add 5 kg of catamine AB and again stirred for 5-10 minutes. Add 1.4 kg glutaraldehyde under constant vigorous stirring to obtain a clear solution. The increase in the amount of more than 7% causes the formation of sludge.

Disinfectant "Roderick" is a transparent colorless or light yellow liquid with a specific smell of terpene compounds. It can be stored in plastic, glass or stainless steel tanks for 3 years.

To determine the availability of fungicidal properties of a 5% solution of the drug was introduced in cylinde (0=10 mm)placed on the surface of the agar medium of čapek-doksa in Petri dishes. Substance the possession is t fungicidal properties, if the sample is placed in the cylinder, the nutrient medium is observed area of the absence of fungal growth (inhibition zone). As the test cultures used the following micromycetes, the most active destructors industrial materials and wood:

Table 1 shows the fungicidal activity of the disinfectant "Roderick".

From table 1 it is evident that the antiseptic drug "Roderick" at a concentration of 5% has antifungal properties, both in relation to individual types investigated the test-cultures of fungi, and in relation to their Association.

Trials of the drug, "Roderick" the ability to exert an inhibitory effect on bacteria was carried out by a special technique in relation to certain types of bacteria using the method of paper disks (D=5 mm). Paper discs were impregnated with the studied substance in various concentrations were placed on the surface of the agar nutrient medium inoculated with the test bacteria culture. As the test cultures used the following bacterial species: Staphylococcus aureus, Bacillus subtilis, Staphylococcus epidermidis, Klebsiella oxytoca, Escherihia coli, Proteus vulgaris, Pseudomonas aeruginosa.

About the degree of inhibitory effect of the drug was judged by the presence of zone of inhibition of growth of test cultures of bacteria formed around the discs with the test substance.

In the tables the 2 shows bactericidal activity of disinfectants means Roderick. The table shows that the disinfectant agent "Roderick" in those concentrations has antibacterial activity against all the studied crops.

Since a concentration of 5% drug "Roderick" showed high antifungal activity (8 to 25 mm, and fungicides is already in the absence of the growth zone, i.e. from 0 mm), additional tests were conducted working concentration of 2.5% of the drug, "Roderick", as well as comparative tests with the closest in composition of the drug "Receptek" (table 3). From table 3 it can be seen that the composition exhibits a higher inhibiting properties than the composition of the prototype, even at lower concentrations.

The composition of the technically reproduced and the active boundary and the averaged values of the initial components.

Sources of information through

Weterynarii, 11, 1996, p.45-47.

2. patent RF №2197994, A61L 2/16, epubl.

Cadorna S. C. Clinical significance of resistance to antimicrobial drugs // Russian medical news. 1998. No. 1. Pp.28-34.

Il, General Microbiology. M., 1987. 566 C.

Alberts B., Bray D., Lewis J., Raff M., Roberts K., Watson, J. Molecular biology of the cell. 3 T.M., 1994. 517 S.

Table 1
No. Species of fungiThe presence of a zone of inhibition of fungal growth around the sample (R, mm)Of product characteristics
1Aspergillus oryzae12fungicide
2Aspergillus niger9fungicide
3Aspergillus terreus12fungicide
4Chaetomium globosum25fungicide
5Paecilomyces variotii20fungicide
6Penicillinum funiculosum18fungicide
7Penicillinum chrysogenum13fungicide
8Penicillinum cyclopium15fungicide
10Alternaria alternata21fungicide
11Fusarium moniliforme11fungicide
12Penicillinum brevicompactum12fungicide
13Penicillinum ochrochloron14fungicide
14Penicillinum martensii13fungicide
15Association of fungi8fungicide

Table 2
No.The test-cultureConcentration,%D zoneinhibition, mmDescriptionmedication
1 Staphylococcus aureus0,520celeberities
2Bacillus subtilis0,520celeberities
4Klebsiella oxytoca0,526celeberities
5Escherihia coli1,016bactericidal
6Proteus vulgaris2,510Sabbatarian
7Pseudomonas aeruginosa2,59Sabbatarian

Table 3
No.Species of fungiThe presence of a zone of inhibition of fungal growth around the sample (R, mm)Of product characteristics
"Receptek" 5%"Roderick" 5%"Roderick" 2,5%
1Aspergillus oryzae3125fungicide
2Aspergillus niger495fungicide
3Aspergillus terreus6128fungicide
4Chaetomium globosum152520fungicide
5Paecilomyces variotii142016fungicide
6Penicillinum funiculosum181813fungicide
7Penicillinum chrysogenum8138fungicide
8Penicillinum cyclopium91512fungicide
9Trichoderma viride8116fungicide
10Alternaria alternata152115fungicide
11Fusarium moniliforme3116fungicide
12Penicillinum brevicompactum812 8fungicide
13Penicillinum ochrochloron11148fungicide
14Penicillinum martensii18130fungicide
15Association of fungi384fungicide

Disinfectant containing katamin AB, isobutylacetate, isopropanol, surfactant and water, characterized in that it additionally contains α-pinene, glutaric aldehyde, glycerin, and as surface-active substances sintimid-5K in the following ratio of components, wt.%:
katamin AB - 15-25
α-pinene - 7-10
isobutylacetate - 7-10
glutaric aldehyde - 5-7
glycerin - 1-5
isopropanol - 1-10
sintimid-5K - 1-2
water - the rest


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1 cl, 1 ex, 2 tbl

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7 tbl

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2 tbl

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4 tbl, 3 ex

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1 tbl

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2 ex, 1 tbl

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4 cl, 6 tbl, 14 ex

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EFFECT: improved preparing method.

1 tbl, 2 ex

Disinfecting agent // 2261089

FIELD: medicine.

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EFFECT: improved and valuable properties of agent.

4 ex

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EFFECT: higher quality of disinfection.

1 ex

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2 tbl, 3 ex

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4 cl, 4 ex

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EFFECT: higher efficiency of application.

6 ex, 1 tbl