Method for controlled mexidol-induced platelet aggregation decrease in experiment

FIELD: medicine, pharmaceutics.

SUBSTANCE: invention refers to medicine, particularly to experimental pharmacology, and concerns methods for controlled platelet aggregation decrease in vitro. That is ensured by administering mexidol to plasma containing 200-220 thousand/mcl of platelets immediately after plasma sampling. Mexidol is used in the concentration of 0.1 to 0.8 mg/ml of an incubation mixture. The incubation is conducted for 10-15 minutes at room temperature.

EFFECT: method provides platelet aggregation to a desired value.

 

The invention relates to medicine, namely to experimental pharmacology.

Known application of three sodium citrate, a mixture of sodium citrate and Epsilon aminocaproic acid solution SDA hypersecreting solution, sodium oxalate, potassium oxalate solution Wintrobe, two sodium salt of ethylenediaminetetraacetate, lithium oxalate, Florida sodium to suppress spontaneous platelet aggregation in vitro [1]. It is known that Mexidol exhibits antiplatelet properties in vivo [2]. However, Mexidol, possess antioxidant properties [3], has not been used for controlled lowering of the aggregation activity of platelets in vitro.

The purpose of the invention - expanding Arsenal of tools to reduce aggregation activity of platelets necessary to the experimental values.

This goal is achieved by applying etilmetilgidroksipiridina succinate (Mexidol) as a means of having the ability to reduce aggregation activity of platelets in a dose-dependent manner.

The invention reduces aggregation activity of platelets in experimental limits.

The method is as follows.

Mexidol is introduced into the platelet-rich plasma directly after its receipt at a concentration of platelets 200-220 thousand/ál. In the incubation with the art creates a concentration of Mexidol from 0.1 mg/ml to 0.8 mg/ml Incubated for 10 to 15 min at room temperature. Get the plasma rich in platelets in which the platelets have required the experimenter activity.

When applying Mexidol identified the following change indicators aggregation activity of platelets (table 1) in comparison with control 1 (control 1 taken as 100%). Control 1 was intact plasma rich in platelets, control 2 plasma rich in platelets, which were administered the same volume of solvent for Mexidol (NaCl 0.85 per cent, pH 7.4).

Table 1
GroupThe degree of aggregationThe rate of aggregationTime aggregation
Control 1 (intact)100%100%100%
Control 2 (saline)105%102%102%
Mexidol 0.002 mg/ml104%104%139%
Mexidol 0,010 mg/ml108% 124%88%
Mexidol 0,020 mg/ml97%101%123%
Mexidol 0,040 mg/ml99%111%90%
Mexidol 0,080 mg/ml95%106%117%
Mexidol 0.100 mg/ml97%96%88%
Mexidol 0,200 mg/ml80%89%56%
Mexidol 0,400 mg/ml47%73%26%
Mexidol 0,600 mg/ml0%39%24%
Mexidol 0.800 to mg/ml0%0%0%
Mexidol 1,000 mg/ml0%0%0%
Mexidol 1,200 mg/ml0% 0%0%

1. Thus, the introduction of Mexidol in the dose of from 0.400 mg/ml or more in platelet-rich plasma causes a significant decrease in maximum platelet aggregation and a significant (p<0.001) decrease in the rate of aggregation, completely suppressing the aggregation in a dose of more than 8,000 mg/ml

2. When the concentration of Mexidol in platelet-rich plasma from 0.400 mg/ml or more, significantly (p<0.001) reduced the time of platelet aggregation.

The proposed method can be used in scientific research to finding and developing new effective ways of reducing aggregation activity. You can use this method to create the necessary level of aggregation activity of platelets in the study of various properties of platelets or influence of drugs on platelets.

References

1. Laboratory methods of hemostasis. Edited by Professor D. of Golberg. -Tomsk, 1980. - 292 S.

2. Zrazhevskaya YEAR Use of Mexidol in the treatment of preeclampsia: author. Diss.... Kida. the honey. Sciences. - Irkutsk - 2001. - 22 S.

3. Klebanov GI, Lubicky O., Vasilyev, O.V., Klimov J.V., Benzolive O., tiplyashin A.S., Thick LM, Promience VK Vladimirov, Y.A. Antioxidant properties of derivatives of 3-oksipiridina: Mexidol, emoxipin and proxipen// Questions of medicines is some chemistry. - 2001, UDC 615.849.19.

The method of controlled lowering of the aggregation activity of platelets, characterized by the fact that in the plasma, containing 200-220 thousand/μl platelets, immediately after its receipt, enter Mexidol in a concentration of from 0.1 to 0.8 mg/ml of incubation mixture, and the incubation carried out for 10-15 min at room temperature.



 

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