Method for increasing radioresistance in mammals

FIELD: medicine.

SUBSTANCE: invention refers to medicine and veterinary science and aims at increasing the radioresistance in mammals. In the period of 12 hours to 20-30 minutes before a radiation exposure and immediately thereafter, 0.11% oil preparation of chlorophyll is introduced into the mammals. Linseed oil is used as an oil base. The preparation is introduced in a dose of 11-12 mg/kg intramuscularly.

EFFECT: method enables increasing the radioresistance and survival rate in the mammals.

1 tbl, 1 ex

 

The invention relates to the field of biological chemistry, radiobiology and can be used in the practice of medical and veterinary measures at contaminated by radioactive substances to the environment to increase the radioresistance of mammals.

The known method antiradiation protection (patent RF №1662042, IPC A61N 2/08, publ. 09.07.1995 g), namely, that the radioresistance of mice increased by the impact of physical factors on the organism, an animal before irradiation put on 3 to 4 hours in camera, reducing the intensity of the geomagnetic field up to 5·10-10t.

The disadvantage of this method is limited its application to a low degree of increase in radioresistance of the body.

An object of the invention is to increase the resistance and survival of mammals at the various types of radioactive destruction of the organism.

The problem is solved through the use of biological radiosensitive of the drug on the basis of derived natural complex of chlorophylls in the practice of medical-veterinary control and supervision.

The essence of the method lies in the fact that in the period from 12 hours to 20-30 minutes before radiation and after radiation exposure mammals enter 0,11%oil drug chlorophy the La, at the same time as oil is used linseed oil, and the drug is administered at a dose of 11-12 mg/kg intramuscularly.

Studies on drug action of chlorophyll on the survival of mice after ionizing irradiation. The objective of the study was to evaluate the radioprotective action of the drug chlorophyll containing in the molecular structure of magnesium. Also during the experiment, we investigated the toxicity, blood picture, the state of the antioxidant system of the organism.

Chlorophylls (a, b) - according to the chemical nature of the esters of dicarboxylic acids of chlorophyllin and two alcohols methyl and unsaturated monohydroxy alcohol phytyl side-chain. Therefore, according to the chemical nomenclature can be defined as titillatingly: chlorophyll a C55H72O5N4Mg, chlorophyll b - C55H70O6N4Mg.

Pigments absorb visible light, but not all, selectively, i.e. each pigment has its own characteristic absorption spectrum. In particular, the most important feature of the absorption spectrum of chlorophyll a and b have two distinct peaks in the red region, respectively 660 and 640 nm in the blue-violet 430 and 450 nm. The minimum absorption lies in the area of green light. This explains the green color of the pigments. Live leaf at chlorophylls wider and aligned absorption spectrum. So, KRA is hydrated maximum absorption of chlorophyll ain the chloroplast has several peaks: 670, 683, 700, 710 nm; chlorophyll b is for wavelengths 650-655 nm. A similar shift towards the long-wave part is true for the blue max. These differences between the absorption spectra of chlorophylls in solution and the sheet is determined by the degree of aggregation of the molecules of the pigment and the nature of their connection with lipoproteidnyi complex in the leafs of the thylakoids.

Chlorophyll b differs from chlorophyllaonly substitution at the third carbon atom in the second pyrrole ring in its molecule the methyl group PA aldehyde. In General, a molecule of chlorophyll due to structural and physico-chemical features able to perform three major functions: to selectively absorb light energy; to store it in the form of electronic excitation energy; photochemically convert the energy of the excited state in chemical energy primary fotovosstanovlenii and vodoochischennya connections.

On the production facilities of LLC "Limex-Pharma" (Moscow) was produced from vegetable raw materials of plant pigment chlorophyll. For experiments, we studied 0,11%drug chlorophyll in solution of linseed oil. Before applying the oil, the drug was dispensed in vials of 20 ml, for sterilization added streptomycin sulfate.

The study of radioprotective is waist conducted pas outbred mice in the amount of 100 heads. Teams were formed: control and test subjects. Animals of both groups were subjected to a single total uniform exposure to gamma radiation from Cs-137 at the "Panorama" with the power 4,3 R/min doses 12,18 Gr (1400 R). The drug was administered intramuscularly in a volume of 0.2 ml (11-12 mg/kg). Was also taken control of irradiation with linseed oil, used for dissolution of chlorophyll. Experimental animals were divided into groups, which were injected oil drug chlorophyll before and after irradiation:

1) the introduction of the drug for 12 h before irradiation;

2) the introduction of the drug for 20-30 min before irradiation;

3) the introduction of the drug in 15-20 min after irradiation;

4) the introduction of the drug for 20-30 minutes before and after 15-20 min after irradiation;

5) the introduction of the drug for 20-30 minutes before and after 15-20 min after irradiation and on the 5th day;

6) the introduction of the drug in 15-20 min after irradiation and on the 5th day;

7) introduction of linseed oil for 12 hours before irradiation;

8) introduction of linseed oil for 20-30 min before irradiation;

9) introduction of linseed oil in 15-20 min after irradiation;

10) control (drug did not enter).

The survival rate of mice at the dose of 1400 R (12,18 Gr) are presented in table 1.

In parallel with the test under irradiation was tested toxicity of the drug and oil on linen 10 outbred white mice-males. The preparation of chlorophyll was in the Eden intramuscularly at a dose of 0.2 ml Survival to 30 days 100%. Vegetable oil was given intramuscularly at a dose of 0.2 ml of Survival to 30 days 100%.

The main indicators of the effectiveness of the drug chlorophyll served the survival of animals within 30 days after irradiation, the lifespan of mice.

The study of the acute toxicity and the pyrogenic action of the drug chlorophyll was carried out in the laboratory of preclinical research Institute of environmental medicine of the Kursk state medical University.

The results of the research. Absolutely lethal dose for mice is dose 7,83 Gr (900 R).

The results of the study of radioprotective properties of the drug chlorophyll presented in table 1.

Survival to 30 days in group 2 (introduction chlorophyll 20-30 minutes before exposure) reached 40%, with a lifespan of up to 68 days, compared with control - 7 days.

Survival to 30 days in group 4 (introduction chlorophyll 20-30 minutes before and immediately after irradiation) reached 20%, with a lifespan of up to 71 days, compared with control - 7 days.

It is noteworthy that in the group of 8 (introduction linseed oil for 20-30 minutes before exposure) and group 9 (introduction of linseed oil in 15-20 min after irradiation), which was used to control exposure with linseed oil, the survival rate of mice SOS is Avila 10% and 10%. The survival rate of the control group 10 was 0%.

In General, evaluating the "work" derived porphyrin - chlorophyll in the "beyond" radiation doses, it should be noted that its use increases the lifespan of mice in comparison with control, effective application to radiation and after radiation exposure.

A comparative clinical study of acute toxicity derived porphyrin - chlorophyll found that intramuscular and intragastric administration maximum dose (3 g/kg) for these routes of administration in mice, the drug does not cause toxic effects. The drug chlorophyll can be attributed to low-toxic drugs.

In the study of pyrogenic reactions in response to intramuscular injection of chlorophyll found that the drug does not cause a statistically significant relative to control group increased body temperature.

The proposed method is experimentally demonstrated that isolated from plant materials preparation chlorophyll has a pronounced radioprotective property in excess of the lethal doses of radiation. Effective when applied before irradiation and after irradiation.

According to the report of the state educational institution of higher professional education "Kursk state medical supply is a ski University from 17.12.2010, "On pre-clinical study of acute toxicity and pyrogenic actions of drugs curcumin (PPL-12K) and chlorophyll (PPL-17TH) production LLC LIMEX-PHARMA" (Moscow)" the drug chlorophyll refers to malotoksicen drugs.

Table 1
GroupQty (PCs)SurvivalTime (day)
4567814172021222324272830345557636871
12345678910111213 1415161718192021222324
110Palo4231
survived6410 0
210Palo421111
survived64 43210
310Palo2311111
survived8543200
410Palo1231111
survived974 32210
510Palo244
survived840 0
610Palo12133
survived97630 0

Continuation of table 1
123456789101112131415161718192021222324
710Palo3 43
survived7300
810Palo2 3311
survived852110
910Palo2 3311
survived852110
1010Palo2323
survived85300

The method of increasing the resistance of the organism mammals in the radioactive defeat, namely, that in the period from 12 hours to 20-30 minutes before radiation and after radiation exposure mammals, e.g. the Yat 0,11%oil preparation chlorophyll, at the same time as oil is used linseed oil, and the drug is administered at a dose of 11-12 mg/kg intramuscularly.



 

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