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Antibody against il-6 receptor. RU patent 2505603.

Antibody against il-6 receptor. RU patent 2505603.
IPC classes for russian patent Antibody against il-6 receptor. RU patent 2505603. (RU 2505603):

C12N15/09 - Recombinant DNA-technology
C07K16/28 - against receptors, cell surface antigens or cell surface determinants
A61K39/395 -
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FIELD: biotechnologies.

SUBSTANCE: invention pertains to the versions of antibodies against IL-6 receptor, variable regions of light and heavy chains of which are modified by introduction of amino-acid replacement. There revealed is a pharmaceutical composition for treating the diseases associated with IL-6 and containing the said version of antibody.

EFFECT: invention allows efficient treatment of the diseases associated with IL-6.

8 cl, 48 dwg, 16 tbl, 20 ex

 

The text of the description is given in facsimile form.

1. Antibody against the receptor IL-6, containing region of the heavy chain and area light chain, in which: (1) CDR1 variable field of the heavy chain consists of amino acid sequence SEQ ID NO:1, in which the Ser in position 1 replaced with Asp, (2) CDR2 variable field of the heavy chain consists of amino acid sequence SEQ ID nos:2, which Thr 9 and Ser in the position 16 replaced by Asn and Gly respectively, (3) CDR3 variable field of the heavy chain consists of amino acid sequence SEQ ID NO:3, (4) CDR1 the variable region of the light chain composed of the amino acid sequence SEQ ID NO:4, where Arg is in position 1 replaced by Gln, (5) CDR2 the variable region of the light chain composed of the amino acid sequence SEQ ID NO:5, which Thr in position 2 and Arg in position 4 of the replaced and Gly Glu respectively, and (6) CDR3 the variable region of the light chain composed of the amino acid sequence SEQ ID NO:6 which Thr in position 5 replaced on Ser.

2. Antibody against the receptor IL-6, containing region of the heavy chain and area light chain, in which: (1) CDR1 variable field of the heavy chain consists of amino acid sequence SEQ ID NO:1, in which the Ser in position 1 replaced with Asp, (2) CDR2 variable field of the heavy chain consists of amino acid sequence SEQ ID nos:2, which Thr 9 and Ser in the position 16 replaced by Asn and Gly respectively, (3) CDR3 variable field of the heavy chain consists of amino acid sequence SEQ ID NO:3, in which the Ser in position 1 and in Thr position 5 were replaced with Val and Il respectively, (4) CDR1 the variable region of the light chain composed of the amino acid sequence SEQ ID NO:4, where Arg is in position 1 replaced by Gln, (5) CDR2 the variable region of the light chain composed of the amino acid sequence SEQ ID NO:5, which Thr in position 2 and Arg in position 4 of the replaced and Gly Glu respectively, and (6) CDR3 the variable region of the light chain composed of the amino acid sequence SEQ ID NO:6 which Gln in position 1 and Thr in position 5 replaced by Gly Arg respectively.

3. Antibody against the receptor IL-6, containing region of the heavy chain and area light chain, in which: (1) CDR1 variable field of the heavy chain consists of amino acid sequence SEQ ID NO:1, in which the Ser in position 1 replaced with Asp, (2) CDR2 variable field of the heavy chain consists of amino acid sequence SEQ ID NO:122, (3) CDR3 variable field of the heavy chain consists of amino acid sequence SEQ ID NO:3, in which the Ser in position 1 and Thr in position 5 were replaced with Ala Leu and respectively, (4) CDR1 the variable region of the light chain consists of amino acid sequence SEQ ID NO:4, where Arg is in position 1 and Gln in position 4 of the replaced with Gln and Glu respectively, (5) CDR2 the variable region of the light chain composed of the amino acid sequence SEQ ID NO:126, and (6) CDR3 the variable region of the light chain composed of the amino acid sequence SEQ ID NO:6 which Gln in position 1 and Thr in position 5 replaced by Gly Arg respectively.

4. Antibody against the receptor IL-6 according to claim 1, where: (7) FR1 variable field of the heavy chain consists of amino acid sequence SEQ ID NO:7, where Arg in position 13, Gln in position 16, Thr is in a position 23 and Thr in position 30 replaced by Lys, Glu, Ala and Ser respectively, (8) FR2 variable field of the heavy chain consists of amino acid sequence SEQ ID NO:8 which Arg in position 8 replaced by Glu, (9) FR3 variable field of the heavy chain consists of amino acid sequence SEQ ID NO:9 which Met in position 4, Leu in position 5, Arg in position 16 and Val in the position of 27 replaced with Il, Ser, Lys and Ala respectively, (10) FR4 variable field of the heavy chain consists of amino acid sequence SEQ ID NO:10, which Gln in position 3 and Ser in position 5 replaced with Glu and Thr respectively, (11) FR1 the variable region of the light chain composed of the amino acid sequence SEQ ID NO:11, where Arg in position 18 replaced the Ser, (12) FR2 variable region of the light chain composed of the amino acid sequence SEQ ID NO:12 which Lys regulation 11 replaced by Glu, (13) FR3 variable region of the light chain composed of the amino acid sequence SEQ ID NO:13, which Gln at position 23, Pro position 24 and Il in the position of 27 replaced with Glu, Ala and Ala respectively, and (14) FR4 the variable region of the light chain composed of the amino acid sequence SEQ ID NO: 14, which Lys in position 10 replaced with Glu.

5. Antibody against the receptor IL-6 in paragraph 2, where: (7) FR1 variable field of the heavy chain consists of amino acid sequence SEQ ID NO:7, where Arg in position 13, Gln in position 16, Thr is in a position 23 and Thr in position 30 replaced by Lys, Glu, Ala and Ser respectively, (8) FR2 variable field of the heavy chain consists of amino acid sequence SEQ ID NO:8 which Arg in position 8 replaced by Glu, (9) FR3 variable field of the heavy chain consists of amino acid sequence SEQ ID NO:9 which Met in position 4, Leu in position 5, Arg in position 16 and Val in the position of 27 replaced with Il, Ser, and Ala Lys respectively, (10) FR4 variable field of the heavy chain consists of amino acid sequence SEQ ID NO:10, in which the Gln in position 3 and Ser in position 5 replaced with Glu and Thr respectively, (11) FR1 the variable region of the light chain composed of the amino acid sequence SEQ ID NO:11, where Arg in position 18 replaced the Ser, (12) FR2 variable region of the light chain composed of the amino acid sequence SEQ ID NO:12, which Lys regulation 11 replaced by Glu, (13) FR3 variable region light chain consists of amino acid sequence SEQ ID NO:13, which Gln at position 23, Pro in position 24 and Il in the position of 27 replaced with Glu, Ala and Ala respectively, and (14) FR4 the variable region of the light chain composed of the amino acid sequence SEQ ID NO:14, which Lys in position 10 replaced with Glu.

6. Antibody against the receptor IL-6 in paragraph 2, where: (7) FR1 variable field of the heavy chain consists of amino acid sequence SEQ ID NO:7, where Arg in position 13, Gln in position 16, Thr is in a position 23 and Thr in position 30 replaced by Lys, Glu, Ala and Ser accordingly, (8) FR2 variable field of the heavy chain consists of amino acid sequence SEQ ID NO:8 which Arg in position 8 replaced by Glu, (9) FR3 variable field of the heavy chain consists of amino acid sequence SEQ ID NO:184, (10) FR4 variable field of the heavy chain consists of amino acid sequence SEQ ID NO:10, in which the Gln in position 3 and Ser in position 5 replaced with Glu and Thr respectively, (11) FR1 the variable region of the light chain composed of the amino acid sequence SEQ ID NO:l1, where Arg in position 18 replaced the Ser, (12) FR2 variable region of the light chain composed of the amino acid sequence SEQ ID NO:12 which Lys regulation 11 replaced by Glu, (13) FR3 variable region of the light chain composed of the amino acid sequence SEQ ID NO:13, which Gln at position 23, Pro in position 24 and Il in the position of 27 replaced with Glu, Ala and Ala respectively, and (14) FR4 the variable region of the light chain composed of the amino acid sequence SEQ ID NO:14, which Lys in position 10 replaced with Glu.

7. Antibody against the receptor IL-6 to 3, where: (7) FR1 variable field of the heavy chain consists of amino acid sequence SEQ ID NO:7, where Arg in position 13, Gln in position 16, Thr is in a position 23 and Thr in position 30 replaced by Lys, Glu, Ala and Ser respectively, (8) FR2 variable field of the heavy chain consists of amino acid sequence SEQ ID NO:8 which Arg in position 8 replaced by Glu, (9) FR3 variable field of the heavy chain consists of amino acid sequence SEQ ID NO:184, (10) FR4 variable field of the heavy chain consists of amino acid sequence SEQ ID NO:10, in which the Ser in position 5 replaced by Il, (11) FR1 the variable region of the light chain composed of the amino acid sequence SEQ ID NO:11, where Arg in position 18 replaced the Ser, (12) FR2 variable region of the light chain composed of the amino acid sequence SEQ ID NO:12 which Lys regulation 11 replaced by Glu, (13) FR3 variable region of the light chain composed of the amino acid sequence SEQ ID NO:13, which Gln at position 23, Pro in position 24 and Ile in the position of 27 replaced with Glu, Ala and Ala respectively, and (14) FR4 the variable region of the light chain composed of the amino acid sequence SEQ ID NO:14, where Lys in position 10 replaced with Glu.

8. The pharmaceutical composition for the treatment of diseases associated with IL-6, containing the antibody on any one of claims 1 to 7.

 

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