SUBSTANCE: application of combination of diterpene alkaloids of aconitum baicalense: napelline, zongorine, mesaconitine, hipaconitine and N-oxide of epinapelline, obtained by extraction with chlorophorm as hemostimulating medication.
EFFECT: medication possesses expressed hemostimulating activity.
2 tbl, 2 ex
The invention relates to medicine, specifically to pharmacology and Hematology.
The high occurrence of anemic conditions and their influence on the duration and quality of life of patients [1, 2], is the basis of wide application gemostimuliruyuschee tools in medical practice.
Many gemostimuliruyuschee tools [1, 2].
The disadvantage of these tools is often their low efficiency and high frequency of adverse effects [3, 4].
Problem solved by the present invention is an expanding Arsenal of highly effective gemostimuliruyuschee funds.
The task is achieved by using the amount of diterpene alkaloids of Aconite Baikal as gemostimuliruyuschee tools.
New in the present invention is the use as gemostimuliruyuschee means amount of diterpene alkaloids of Aconite Baikal.
Used original means amount of diterpene alkaloids of Aconite Baikal was developed and received NII pharmacology" SB RAMS (Tomsk) in conjunction with the National research Irkutsk State Technical University (Irkutsk, Russia). Diterpenoid alkaloids were extracted from raw materials (herbs Aconite Baikal) in the form of free bases by extraction with chloroform, OTDELA is of n and identified according to standard methods .
In practical medicine for the treatment of anemic States, representing as independent disease, and pathological syndrome, occurring in various diseases, the most widespread means on the basis of recombinant forms of natural regulators of haematopoiesis (cytokines): erythropoietin, granulocyte colony-stimulating factor, granulocytemacrophage colonies-stimulating factor [1-3]. When this protein nature of the specified substance determines the existence of the only possible - parenteral route of its introduction into the body and a significant risk of side effects and complications [4, 5], due to the immunogenicity of these growth factors.
At the same time in NII pharmacology" SB RAMS was shown the principal possibility and high efficiency management functions of mesenchymal stem cells with the help of diterpene alkaloids of Aconite Baikal . When oral detected marked stimulation of stromal progenitor cells of the skin, accompanied by accelerating the process of regeneration of the surface tissues .
While the effect of diterpene alkaloids of Aconite Baikal on the regeneration of hematopoietic tissue and the possibility of stimulation of hematopoiesis due to the activation of rodonachalnik the blood cells is not known. The experiment showed unexpected results.
The fact of the use amount of diterpene alkaloids of Aconite Baikal with achieving a new technical result consists in stimulation of hemopoiesis, for the specialist is not obvious.
The new properties are not derived explicitly from the prior art in this field and is not found in the patent and scientific literature.
The present invention can be used in medicine.
Based on the above, you should consider the claimed solution meets the criteria of "Novelty", "Inventive step", "Industrial applicability".
The experiments were conducted on 62 mice CBA, Animals obtained from the nursery of the Department of experimental biomedical modeling NII pharmacology" SB RAMS.
Studies were performed in accordance with good laboratory practice (GLP), the Order of healthcare of the Russian Federation No. 708n from 23.08.2010 "On approval of rules of good laboratory practice", the Federal Law of April 12, 2010 №61-FZ "On circulation of medicines", the "guidelines for the study gemostimuliruyuschee activity pharmacological substances" (2002) .
Used the original tool was obtained from the herb Aconite Baikal. The aboveground part of the plants collected during the flowering period the Irkutsk region, were ground to particle size less than 5 mm, were treated with sodium carbonate solution and loaded into percolator. 2 hours after load filled him in 2000 ml of chloroform. Extraction with chloroform was conducted on a laboratory installation of continuous operation, consisting of percolator film evaporator and direct the fridge. Continuous circularity solvent is conducted at a speed of 2 l/hour Duration of exhaustive extraction was 5 days. The concentrated chloroform extract of percolator merged through the bottom valve of percolator and were concentrated in the evaporator. The obtained chloroform extract of alkaloidal was purified from alcaloide compounds by extraction with an aqueous solution of sulfuric acid. Further purification of the amount of alkaloids they were re-chloroform extraction from sulfuric acid extract after neutralization and its alkalizing to pH=9-10. To get rid of traces of alkali was washed with 50 ml water. Then the chloroform extract was filtered through a desiccant (15 g anhydrous sodium sulfate). At the last stage the received amount of alkaloids was evaporated to dryness. The product yield was 0.23%. Plant-derived agent, contained an amount of alkaloids: kapellen, somorin, mesaconitine, hypaconitine and N-oxide 12-epinepherine. Received alkaloids diluted distillirovanna the th water and brought them Concentratio to 0.0005%.
The effectiveness of stimulation of hemopoiesis was determined on the model of cytotoxic mielosupression in accordance with the methodological guidelines for the study of gemostimuliruyuschee activity pharmacological substances .
Cytostatic myelosuppression was modeled by a single intraperitoneal injection of a solution of 5-fluorouracil (5-FU)1/2the maximum tolerated dose (MTD) (114 mg/kg). Starting from the next day after administration of cytostatic mouse experimental group received per os 0,0005% - aqueous solution the amount of alkaloids 0.05 mg/kg 1 time per day for 5 days. The control animals under similar conditions was injected solvent (distilled water) equivalent volume (0.2 ml).
5, 8 th and 12-th day after injection of cytostatic animals were killed by inhalation of CO2. From experimental and control mice by standard methods was determined by the content of reticulocytes in the peripheral blood, as well as indicators of bone marrow hematopoiesis (the total number of myelokaryocytes, myelogram) . Content commiteeman precursor cells erythropoiesis (CFU-e) and stromal mechanical (CFU-f) in the bone marrow, as well as products eritropoeticescoe active substances (EPA) stick and non-stick items gemopoeticescoe microenvironment (SHM) was determined by culture is erodov in vitro .
Statistical data processing was performed by the method of variation statistics using t-student criterion. In case of deviation of the distribution in samples from a normal to evaluate the reliability of differences was applied nonparametric test of Wilcoxon signed-Mann-Whitney.
In the experiment, the introduction of 5-FU resulted in developing a deep and prolonged depression of erythroid germ blood. Throughout the timeframe of the study was to decline the content of eritrotsitov in hematopoietic tissue and the number of reticulocytes in the peripheral blood (up 10.2% 0,0% from the background on the 5th day, respectively). However, had expressed compensatory reaction from the pool of hematopoietic precursors. Registered the increase in the number of bone marrow CFU-e (table 1), associated with increased production of hematopoietic cells (EPA) stick and non-stick by myelokaryocytes. In addition, it was revealed increased functional activity of mesenchymal precursors (table 2), which plays an important role in the recovery of corrupted data cytotoxic agent SHM .
Introduction alkaloids led to a significant increase in the intensity of the recovery process of erythropoiesis. An increase in the content of eritrotsitov in the bone marrow, which is on the 8th with the t of the experiment was reached levels of background values and were 190,4% from the similar values in animals cytotoxic control (table 1). Reflection regenerate the hematopoietic tissue has been the emergence of a significant number of reticulocytes in the peripheral blood on the 5th day (35,63% of the background) and the increase in their number in the remaining observation period: up to 1151,0% and 237,7% of control for the 8-th and 12-th day, respectively.
Investigation of the mechanisms gemostimuliruyuschee actions alkaloidsetoposide drug revealed the dependence of the formation of the blood picture and bone marrow from the state of the pool parental bone marrow cells. The introduction of the solution of the pharmacological agents were accompanied by a sharp increase in the content of erythroid precursors in hematopoietic tissue on the 5th and 12th day experience (up to 132.6% and 347,6% of the cytotoxic control, respectively) (table 1).
The reaction of the haematopoietic precursors were largely determined by the change in the secretory functions of the stromal elements of the SHM. During the experiment were recorded increase production eritropoeticescoe active substances stick myelokaryocytes on the 5th, the 8th day of the study. At the same time, the alkaloids of Aconite Baikal does not affect the mobile fraction of the nuclears bone marrow. Production of EPA non-stick by surrounding cells remained at the level of the control values (table 2).
In addition, in the in-depth study of the influence of the investigated compounds the substances on the local mechanisms of regulation of erythropoiesis was installed another feature of the pharmacological action of alkaloids in respect of stromal progenitor cells from the bone marrow. The introduction of the drug on the background modeling cytotoxic mielosupression resulted in lower levels of fibroblastic SOME 5 day experience 1.7 times compared with the control group (table 2). Based on information in the literature  on the stimulating effect of the alkaloids of Aconite Baikal on mesenchymal precursors with predominant activation of processes of differentiation, it follows that the development of the identified phenomenon could be the result of the ripening acceleration data of the parental cells. Where compliance with the dynamics of changes of the studied parameter in such cytostatic control (table 2), when there is a significant part of CFU-f in regeneration SHM . specifies the compensatory nature of drug induced shifts. Moreover, the development of the described reactions from mesenchymal precursors, probably served as the basis described above (stimulation of secretion EPA stick myelokaryocytes) enhance the role of the stromal component of the SHM in the restoration of suppressed 5-FU erythropoiesis due to the formation of de novo functionally active adherent of myelokaryocytes.
Thus, the results obtained indicate expressed gemostimuliruyuschee properties, the amount of diterpene alkaloids of Aconite Baikal in their oral use. This mechanismo the acceleration of regeneration of hematopoietic tissue is the activation of the parental hemo poetic cells by direct action on them alkaloids , and as a result of increased feeder capacity stromal compartment SHM. In addition, identified the importance of changes in the functional activity fibroblastic predecessors in the implementation gemostimuliruyuschee effects of the proposed tools.
1. Metcalf D. Hemopoietic growth factors. 1. // The Lancet. - 1989. - Vol.15. - P.825-827.
2. Mashkovsky PPM Medicines: 15-ed. - M: PA "Publishing house New Wave", 2008. - 1206 S.
3. Volkova MA.. Shirin A.D. Erythropoietin in the treatment of anemia in cancer // Hematol. and transfusion. - 1997. - V.42. No. 6. - Pp.33-36.
4. Anderson J.A. Allergic reactions to drugs and biologic agents. JAMA. - 1992 - vol.268. - p.2845-2857
5. Pogodaev N.N., Gapova C., Vereshchagin A.L., Gorshkov A.G., Semenov A., study of the alkaloid composition of some species of Siberian aconito // Rast. resources, issue 2, 2000, S-84.
6. Sushkov GN,, Krapivin V., Nesterov Y.,, Povetieva T.N., Zhdanov V., Suslov NI, T. Fomin.. Udut E.V., Miroshnichenko, L.,, Kamanina E.V., A.A. Semenov, S. Kravtsova, Digi A.M. regenerative Mechanisms of action of diterpene alkaloids of Aconite Baikal // bul, experimental. Biol. and medicine. - 2012. No. 6. - S-827.
7. Digi A. M., Zhdanov V., Goldberg V.E. and other guidelines for the study gemostimuliruyuschee activity pharmacological substances // Bulletin of the scientific expertise and state control Le is arctonyx funds. - 2002. No. 1 (9). - P.29-32.
8. Goldberg ED, Digi A. M., Shah V.P. Methods of tissue culture in Hematology. - Tomsk: Publishing house of Tomsk state University, 1992. - 272 S.
9. Dygai A.M., Zyuz'kov G.N., Zhdanov V.V., et al. Effect of Transplantation of Peripheral Blood Mononuclears Obtained Using Granulocytic Colony-Stimulating Factor and Hyalu-ronidase on Hemopoietic Regeneration of Tissue during Myelosuppression // Bulletin of Experimental Biology and Medicine. - 2009. - Vol.148. No. 1. - P.120-125.
|Dynamics of the content of erythroid cells in the bone marrow, reticulocytes in peripheral blood CFU-e in the bone marrow of mice CBA/CaLac after injection of distilled water (1) or the amount of alkaloids (2) against a single injection of 5-fluorouracil in1/2MTD, (X±m; R)|
|The timeframe of the study day||Group||Erythroid cells|
|The reticulocytes (%)||CFU-e (×105the nuclears)|
|before the introduction of||1,77±0,32||36,00±2,05||22,00±3,02|
|Note: * - differences significant in relation to the background,|
|# - the difference is significant compared to the group treated with distilled water by infusion of 5-fluorouracil (1)|
|Dynamics of the level eritropoeticescoe activity in supernatant from non-stick, stick of kariolou bone marrow, the content of CFU-f in the coast is the second brain of mice CBA/CaLac after injection of distilled water (1) any amount alkalai Dov (2) against a single injection of 5-fluorouracil in 1/2MTD, (X±m; R)|
|The timeframe of the study day||Group||EPA from sticking to the myelokaryocytes||EPA from the non-stick myelokaryocytes||CFU-f (2.5×105the myelokaryocytes)|
|before the introduction of||2,33±0,33||4,0±0,73||9,00±0,73|
|Note: * - differences significant in relation to the background,|
|# - the difference is significant compared to the group treated with distilled water by infusion of 5-fluorouracil (1)|
The use amount of diterpene alkaloids herb Aconite Baikal: kapellen, somorin, mesaconitine, hypaconitine and N-oxide 12-epinepherine obtained by extraction with chloroform, as gemostimuliruyuschee funds.
SUBSTANCE: invention refers to medicine, particularly to transfusion medicine, namely to a method for preparing polymer modified haemoglobin by the step multifunctional condensation of purified haemoglobin recovered from concentrated red cells in the mixed oxy/deoxy form with a synthetised multifunctional cross-linking agent that is glutaric aldehyde with glutamic acid and sodium glutamate, wherein the first stage of the reaction involves the intramolecular chemical cross-linking of a haemoglobin molecule labile in an aqueous solution; the second stage involves the intramolecular cross-linking of the ready modified haemoglobin molecules to form polymer modified haemoglobin.
EFFECT: invention provides preparing more effective polymer modified haemoglobin in the form of the multifunctional blood substitute having the effective oxygen transfer function, anti-shock and haemodynamic action able to initiate own blood formation.
3 cl, 1 ex, 2 tbl
FIELD: medicine, pharmaceutics.
SUBSTANCE: presented group of inventions refers to immunology and medicine. There are presented compositions containing a digoxin antibody for weakening the action of tumour necrosis factor on a cell adhesion molecule and for weakening the TNF induction of ICAM, VCAM and E-selectin expression in an endothelial cell. What is presented is a method for weakening the cytokine-induced surface expression of the cell adhesion molecule involving the stage of cell contact to a digoxin antibody composition.
EFFECT: group of inventions provides the effective weakening of the cytokine-induced TNF-α surface expression of the ICAM, VCAM and E-selectin molecules due to contacting the cell in need thereof with the digoxin antibody composition.
5 cl, 12 dwg, 6 tbl, 4 ex
SUBSTANCE: invention refers to medicine, namely to - physiotherapy, to infectious diseases. The method involves the integrated use of drug preparations, magnetic and laser therapy. The laser therapy is differentiated depending on the severity, modified leukocytal intoxication index (mLII), malondialdehyde (MDA), lipid hydroperoxide (LHP), antioxidant activity (AOA), interleukin-1β (IL-1β), tumour necrosis factor (TNF-α). The mild severity, mLII within the range of 2.8±0.09-3.44±0.07, MDA 3.7±0.08-4.2±0.07mcm/ml, LHP 10.1±0.1-11.3±0.09 mcm/ml, AOA 0.489±0.005-0.390±0.007, IL-1β 25.3±0.5-26.71±0.3 pg/ml; TNF-α 37.1±0.5-45.7±0.8 pg/ml require the daily percutaneous exposure to a constant magnetic field and low-intensity laser light of power 55 mWt, wave length 0.89 mcm, pulse frequency 80 Hz in the morning hours. The contact scanning exposure covers a projection of thymus, regional lymphatic nodes and great vascular pedicle. The length of the exposure makes 60 seconds per each region. Then, an inflammatory centre is exposed to pulse red light of wave length 0.65 mcm, output pulse power min. 5 Wt, pulse frequency 80 Hz, modulation frequency of light-emitting diodes 8 Hz generated by a light guide tip. The length of the exposure is 120 minutes. The therapeutic course consists of 5 procedures. The moderate severity, mLII 4.18±0.08-6.06±0.07, MDA 4.9±0.03-5.6±0.02 mcm/ml, LHP 12.3±0.08-14.7±0.07 mcm/ml; AOA 0.345±0.007-0.315±0.006, IL-1β 27.1±0.2-28.1±0.1 pg/ml, TNF-α 57.7±0.9-72.1±0.5 pg/ml requires the daily percutaneous exposure to the constant magnetic field and low-intensity laser light of power 60 mWt, wave length 0.89 mcm, pulse frequency 80 Hz in the morning hours. The contact scanning exposure covers a projection of thymus, regional lymphatic nodes and great vascular pedicle. The length of the exposure makes 90 seconds per each region. Then, an inflammatory centre is exposed to pulse red light of wave length 0.65 mcm, output pulse power 7 Wt, pulse frequency 80 Hz, modulation frequency of light-emitting diodes 8 Hz generated by the light guide tip. The length of the exposure is 150 minutes. The therapeutic course is 7 procedures. The severe condition, mLII 7.76±0.08-8.06±0.07, MDA 7.1±0.03-11.6±0.02 mcm/ml, LHP 16.3±0.08-19.7±0.07 mcm/ml; AOA 0.310±0.007-0.294±0.006, IL-1β 30.1±0.2-31.1±0.1 pg/ml, TNF-α 76.7±0.9-85.1±0.5 pg/ml requires the daily percutaneous exposure to the constant magnetic field and low-intensity laser light of power 60 mWt, wave length 0.89 mcm, pulse frequency 80 Hz in the morning hours The contact scanning exposure covers a projection of thymus, regional lymphatic nodes and great vascular pedicle. The length of the exposure makes 120 seconds per each region. Then, an inflammatory centre is exposed to pulse red light of wave length 0.65 mcm, output pulse power 9 Wt, pulse frequency 80 Hz, modulation frequency of light-emitting diodes 8 Hz generated by the light guide tip. The length of the exposure makes 180 seconds. The therapeutic course consists of 9 procedures. For the first five days, all the patients are prescribed with heparin electrophoresis by common technique during afternoon.
EFFECT: method reduces a rate of recurrence.
3 tbl, 3 ex
FIELD: medicine, pharmaceutics.
SUBSTANCE: invention refers to chemical-pharmaceutical industry, and represents an immunomodulator for treating chronic hepatitis, hepatic cancer, lymphatic sarcoma, chronic leukemia, and for improving the functions of liver and blood-forming organs, for enhancing the immunobiological body characteristics, prepared by mixing 1000 ml of an aqueous infusion of sandy everlasting blossom, pepper mint herb and chicory herb with 50 ml of bovine serum containing leukaemia oncovirus antibodies, 20 ml of wild rosemary infusion, 40 g of ascorbic acid, 2 g of sorbic acid, 0.2 g of folic acid until the ingredients are dissolved completely, with adding 60 g of liver powder, 30 g of lymphatic node powder, 30 g of young bovine spleen powder; the prepared solution is settled at room temperature for 24 hours, then kept at a boiling water bath for 30 minutes and cooled for 6-8 hours at room temperature; the settled solution is filtered, wherein the aqueous herbal solution is prepared by mixing equal proportions of the separately prepared aqueous infusions of 40 g of pepper mint herb in 1000 ml of water, 30 g of sandy everlasting blossom in 1000 ml of water and 30 g of chicory herb in 1000 ml of water, while the wild rosemary infusion is prepared by infusing 60 g of ground wild rosemary blossom in 1000 ml of 70% purified ethanol.
EFFECT: invention provides creating the high-efficacy agent and reducing the length of treatment.
SUBSTANCE: invention refers to veterinary science, and may be used for normalising spontaneous erythrocyte aggregation (SEA) in newborn piglets suffered acute hypoxia at birth. That is ensured by prescribing crezacin 4 mg/kg a day of fed-on plan in the morning for five days combined with gamavit 0.03 mg/kg intramuscularly once a day in the morning for five days in the newborn piglets suffered acute hypoxia at birth.
EFFECT: method enables normalising SEA in the newborn piglets, normalising weight gain, reducing death loss and improving the quality of meat products.
SUBSTANCE: invention relates to veterinary, and can be applied for normalisation of spontaneous erythrocyte aggregation (SEA) in piglets with dairy-vegetative nutrition with bronchitis. For this purpose to piglets of diary-vegetative nutrition with bronchitis simultaneously prescribed are crezacine in dose 4 mg/kg per day in scheme of drinking in the morning for five days and gamavit in dose 0.03 mg/kg per day intramuscularly one time per day, for five days.
EFFECT: method makes it possible to normalise level of SEA in piglets, reduce mortality, normalise weight gain, and increase quality of obtained from them in future meat production.
SUBSTANCE: invention refers to veterinary science, and may be used for normalising spontaneous erythrocyte aggregation (SEA) in sows with arthritis. That is ensured by prescribing crezacin 4 mg/kg a day of fed-on plan in the morning for five days combined with gamavit 0.03 mg/kg intramuscularly once a day in the morning for five days.
EFFECT: method enables normalising SEA in sows, reducing death loss and improving the quality of the produced piglets and meat products.
SUBSTANCE: invention relates to bicyclosubstituted pyrazolon azo derivatives of formula
or pharmaceutically acceptable salts thereof, intermediate compounds of formula ,
as well as methods for production thereof, a pharmaceutical composition containing a compound of formula (II), and use thereof as a therapeutic agent, which is a thrombopoietin (TPO) mimetic, as well as use thereof as agonists of the thrombopoietin receptor. Values of substitutes in formulae (I) and (IA) are given in the claim.
EFFECT: obtaining bicyclosubstituted pyrazolon azo derivatives.
12 cl, 58 ex
SUBSTANCE: invention refers to veterinary science, and may be used for normalising spontaneous erythrocyte aggregation in suckling piglets with arthritis. That is ensured by prescribing crezacin 4 mg/kg a day of fed-on plan in the morning for five days combined with gamavit 0.03 mg/kg intramuscularly once a day in the morning for five days in the suckling piglets with arthritis.
EFFECT: method enables normalising spontaneous erythrocyte aggregation in piglets, reducing death loss, normalising weight gain, and improving the quality of meat products.
SUBSTANCE: invention refers to veterinary science, and may be used for normalising spontaneous erythrocyte aggregation in newborn piglets with dyspepsia. That is ensured by prescribing crezacin 4 mg/kg a day of fed-on plan in the morning for five days combined with gamavit 0.03 mg/kg intramuscularly once a day in the morning for five days.
EFFECT: method enables normalising spontaneous erythrocyte aggregation in newborn piglets, normalising weight gain, reducing death loss and improving the quality of meat products.
SUBSTANCE: method of obtaining rutin from vegetative mass of buckwheat includes the following stages: dried, crushed buckwheat is subjected to extraction with 70% ethyl alcohol, thickened, filtered, dried, purified from admixtures by means of organic solvents, dissolved, subjected to hot filtration, crystallised, with crushed vegetative buckwheat mass being before extraction subjected to uncoiling, extraction with 70% ethyl alcohol on boiling water bath, filtration and re-extraction with 70% ethyl alcohol, re-filtration, obtained alcohol extracts are concentrated on water bath under vacuum and dried, twice purified from admixtures by means of organic solvents, as organic solvents used are diethyl ester and ethylacetate. After crystallisation, re-crystallisation is carried out with hot 70% ethyl alcohol with hot filtering through paper filter, with further cooling until rutin crystals precipitate, filtration under vacuum and drying in the open air.
EFFECT: application of the claimed method makes it possible to obtain rutin with high degree of purification.
SUBSTANCE: composition to be used for the purpose of inhibiting the infecting ability of viruses accompanying viral infections, including cold with a primary infection caused by rhinoviruses, adenoviruses and/or coronaviruses, influenza, and a viral infection caused by retroviruses; the above composition contains an aqueous extract of Ribes leaves and branches. Using the aqueous extract of Ribes leaves and branches for preparing a drug preparation for inhibiting the infecting ability of viruses accompanying viral infections, including cold with the primary infection caused by rhinoviruses, adenoviruses and/or coronaviruses, influenza, and the viral infection caused by retroviruses The composition containing the aqueous extract of Ribes leaves and branches is effective for inhibiting the infecting ability of viruses accompanying viral infections, including cold with the primary infection caused by rhinoviruses, adenoviruses and/or coronaviruses, influenza, and the viral infection caused by retroviruses.
EFFECT: improved properties of the composition.
14 cl, 3 dwg, 1 ex
SUBSTANCE: method involves the subcutaneous injections of donor animals' hyperimmune blood serum containing antihemagglutinin in titres to herpes virus-3 1:1280, to rednose virus - min. 1:256, to viral diarrhea -1:1024 and to adenovirus -1:128; in a dose of 2.0 ml/kg every 24 hours until recovered completely; besides, 20-30 minutes before feeding, a pytopreparation of 70% alcohol tincture of purple echinacea (Echinacea purpurea L) herb and blossom, common pine (Pinus sylvestris) buttons, horseheal (Inula helenium) roots and rhizomes, common licorice (Glycyrrhiza glabra L.) roots and harmala shrub (Peganum harmala) herb taken in relation 1:1:1:1:0.5 in the amount of 7-8% aqueous solution is administered in a dose of 3.0-3.5 ml/kg of body weight every 12 hours until recovered completely.
EFFECT: higher clinical effectiveness and natural body resistance.
4 tbl, 3 ex
SUBSTANCE: invention represents a method for biomechanical stimulation of collagen synthesis in skin cells and reduction of skin small lines and wrinkles involving: (a) forming a polymer composition containing a first polymer and a second polymer that are dissolved or dispersed in a solvent system containing water, wherein the above first polymer represents a water-soluble or water-dispersible anionic polymer able to be reduced after solvent evaporation, and wherein the above second polymer represents a water-soluble or water-dispersible cationic polymer able to form a polymer complex able to bind to a skin surface, (b) applying the polymer composition on a first skin region and a second skin region, wherein the first and second regions are separated from each other at a specified distance by at least one small line or wrinkle in between; and (c) drying the polymer composition in the first and second skin regions in the first and second skin regions so that water evaporates in the solvent system and the polymer composition is initiated to be reduced in the first and second skin regions, wherein the above reduction creates a tension over the skin surface between the first and second regions; the tension provides the biomechanical stimulation of collagen in skin cells and reduction of skin small lines and wrinkles.
EFFECT: invention provides the biomechanical stimulation of collagen in skin cells and reduction of skin small lines and wrinkles.
16 cl, 7 dwg
SUBSTANCE: as active ingredients, the presented toothpaste contains troxerutin in the amount of 0.1-0.3 wt %, ectoin in the amount of 0.01-0.1 wt % and nicotinamide in the amount of 0.05-0.1 wt %, as well as a biologically active additive containing aloe vera and herbal infusion; an anti-caries additive and target additives in the amounts to make the above functions be fulfilled; a cosmetically acceptable base containing an abrasive, a humectant, a thickening agent, a cleansing and foaming agent, a sweetening agent, and water as a medium.
EFFECT: toothpaste composition provides the effective regenerative action on periodontal tissues alongside with the high cleansing properties of the toothpaste.
14 cl, 5 tbl, 2 ex
SUBSTANCE: combined dental whitening and oral therapeutic composition for delaying and inhibiting dental caries, delaying or inhibiting demineralisation and promoting dental remineralisation. The composition contains an effective amount of a whitening agent and arginine in a salt form in the amount of 0.1 wt % to 50 wt % with the whitening agent and arginine dispersed in a matrix material.
EFFECT: improved properties of the composition.
17 cl, 11 ex
SUBSTANCE: wet wipe for cleansing the skin or hair of a user comprises: a wipe substrate and a single-phase liquid composition containing from 0.05% (by weight of the composition) to 50% (by weight of the composition) of at least one water dispersible silicone and from 50% (by weight of the composition) to 99.95% (by weight of the composition) of water. Silicon represents modified sorbitan siloxane having the following structure 1, wherein R1 represents an alkyl group having 7 to 21 carbon atoms, R2, R3 and R4 are independently specified in a group consisting of H and structure 2, wherein at least one of the radicals R2, R3 or R4 represents structure; a is an integer ranging from 0 to 200; b is an integer ranging from 1 to 10; z is an integer ranging from 1 to 10; c is an integer ranging from 0 to 10; d is an integer ranging from 5 to 20; n is an integer ranging from 7 to 17; e is an integer ranging from 0 to 30; f is an integer ranging from 0 to 30; g is an integer ranging from 0 to 30, wherein the sum of e, f, and g is an integer ranging from 9 to 50; and wherein the ratio of hydroxyl to carboxyl group ranges from 4:1 to 2:1.
EFFECT: invention provides user's pleasant sensations, improves the skin and hair with using no hard surfactants.
20 cl, 7 ex, 8 tbl
FIELD: food industry.
SUBSTANCE: method involves preliminary milling of berries into particles sized 2-5 mm; then berries surface treatment with enzyme preparations. Before ultrasound exposure one performs the components soaking in well or purified water during 6-8 hours at a temperature of 35-40°C, after treatment one proceeds with the extract cooling and filtration.
EFFECT: method allows to produce an extract with enhanced biological activity, nutritive value and bactericidal effect and to increase the extractive, flavouring and colouring substances yield.
SUBSTANCE: invention refers to medicine, namely physiotherapy. A method involves the administration of chophytol, diet therapy, magnetic therapy and exposure to electric current. The magnetic therapy is performed by a low-frequency sinusoidal alternating magnetic field. The exposure covers a collar zone and liver for 10-15 minutes. A continuous mode, frequency 50 Hz, grade I-II magentic induction are specified. The magnetic therapy is immediately followed by the exposure to complex-modulated pulse electric current with a neural-like pulse shape. The exposure is generated at the level ThVII - ThXI, paravertebrally to the right, and cover skin projections within the liver and gallbladder transversally. For this purpose, the electrodes are placed in 6 procedure fields: 1 - paravertebrally to the right at the level of the ThVII spine, 2 - paravertebrally to the right at the level of the ThXI spine, 3 - in a projection of the liver on a front surface of the body, 4 - in a projection of the liver on a back surface of the body, 5 - in a projection of the gallbladder on the front surface of the body, 6 - in a projection of the gallbladder on the back surface of the body. The 1st and 2nd electrodes are connected to the channel IV, while the 3rd and 4th electrodes - to the channel I, and the 5th and 6th electrodes are connected to the channel II. The channels I, II work in a frequency-drift group mode. The channel IV work in a continuous mode at fixed frequency 20 Hz. The current intensity is adjusted to apparent vibration sensation. Duration of a session is 10-15 minutes. The therapeutic course is 10-12 daily procedures.
EFFECT: method improves the motor function of the gall bladder ensured by the improved cholekinesis, hepatic circulation and cholestasis elimination.
2 ex, 3 tbl
FIELD: medicine, pharmaceutics.
SUBSTANCE: invention refers to pharmaceutical industry, namely a method for preparing an agent possessing anticoagulant, hypotensive and diuretic action. The method for preparing the agent possessing anticoagulant, hypotensive and diuretic action, consisting in the fact that milled dark mullein is extracted in 60% ethanol by re-percolation in a cluster of 3 unequally batched percolators; the prepared extract is settled, filtered, boiled down and dried in a vacuum drier in the certain environment.
EFFECT: agent prepared by the above method possess pronounced anticoagulant, hypotensive and diuretic action.
FIELD: medicine, oncology, amino acids.
SUBSTANCE: invention relates, in particular, to the development of an antitumor preparation based on natural substances. Invention relates to an amino acid preparation comprising at least one modified essential amino acid obtained by treatment of amino acid by ultraviolet radiation (UV) at wavelength 250-350 nm for 12-80 h at temperature 15-30oC or with ozone at temperature 15-25oC. The modified amino acid has no toxicity for health cells. Also, invention relates to a method for preparing such preparation. Invention provides the development of an antitumor preparation based on modified amino acids and expanded assortment of antitumor preparations being without cytotoxicity for normal cells.
EFFECT: valuable medicinal antitumor properties of preparation.
8 cl, 4 tbl, 2 dwg, 4 ex