Method for prediction of infectious inflammation of postoperative wound in external fixation of long bones

IPC classes for russian patent Method for prediction of infectious inflammation of postoperative wound in external fixation of long bones (RU 2504774):

G01N33/48 - Biological material, e.g. blood, urine (G01N0033020000-G01N0033140000, G01N0033260000, G01N0033440000, G01N0033460000 take precedence;determining the germinating capacity of seeds A01C0001020000); Haemocytometers (counting blood corpuscules distributed over a surface by scanning the surface G06M0011020000)

Another patents in same IPC classes:

Method of predicting unfavourable course of locally spread forms of human papilloma virus-associated cervical cancer / 2504326

Invention relates to field of medicine, namely to oncogynecology. Immunological parameters are determined: absolute number of lymphocytes (lim_ab), TNFα (TNFα-k) and IL-10 (IL-IL10_k) in peripheral blood, as well as TNFα (TNFα-c) and IL-4 (IL-4_c) in cervical mucus. On the basis of obtained data parameter F(x) is calculated by formula: F(x): P(x)=9.265+1,749x(lim_ab)+0.013×(IL-4_c)+0.005×(IL-10_k)-0.410×(TNFα-k)-0.206×(TNPα-c). If value F(x) is higher than 0.555, unfavourable course of tumour process is predicted, in case if it is lower than 0.555, favourable course is predicted.

Method for assessing conditions of proliferative cervical diseases / 2503960

Method is staged: 1) cervical canal scrapes and biopsy materials of the uterine cervix are used to recover DNA of human papilloma virus; 2) virus genotype is determined; 3) patients with HPV 16 positive CIN are sampled; 4) real-time PCR is used to determine the number of virus DNA copies and a degree of its integration with a host gene (a physical status); 5) a threshold level of the viral load is stated taking into account the physical status of the virus (6.5 lg HPV DNA copies 16 per one cell); 6) the patients are classified according to the determined threshold viral load and physical status of the virus (the episomal and integrated form); 7) the poorly predicted patients having the high viral load (≥6.5 lg DNA copies per one cell) are detected in the episomal form of the virus, while the low load (<6.5 lg DNA copies per one cell) is shown by the integrated form of the virus.

Method for prediction of individual risk of bronchial asthma in regions with high and low incidence of helminth infections / 2503959

Presence or absence of opisthorchiasis is stated; and polymorphous versions of genes and a possibility of referring an individual to a group with a low or high risk of bronchial asthma are found. If observing one of any risk combinations in accordance with the SOCS5-IFNG-opisthorchiasis model in the patient, the high risk of bronchial asthma is diagnosed. If observing one of any protective combinations in accordance with the IL4-TBX21-SOCS5 model in the patient, the low risk of bronchial asthma is diagnosed.

Method of individual quantitative assessment of ischemic heart disease development / 2503405

Invention relates to cardiology, pathophysiology, biochemistry and pharmacology. To assess development of ischemic heart disease value of discriminant function is calculated by a set of parameters: age, height, weight, Quetelet index, Broca's index, abdominal obesity, triglycerides, total cholesterol, high density lipoprotein cholesterol, low density lipoprotein cholesterol, atherogenicity index, arterial pressure, glucose, alanine aminotransferase, aspartate aminotransferase, lactate dehydrogenase, α-amilase, total protein, albumin, uric acid, urea, creatinine, creatine kinase, alkaline phosphatase, total bilirubin, direct bilirubin, ADP-induced platelet aggregation, collagen-induced platelet aggregation, platelet number, mean platelet volume, number of red blood cells, hematocrit, hemoglobin, mean corpuscular volume, RDW-CV, average content of hemoglobin in blood, number of white blood cells, percent content of neutrophil granulocytes, percent content of eosinophils, percent content of basophils, percent content of lymphocytes, percent content of monocytes, mean content of hemoglobin on red blood cell. Conclusion about initial stage of IHD development, 50% risk of disease or 100% development of IHD is made on the basis of obtained value.

Method for assessing disturbance of transamination in plasmodiotrophoblast in third trimester of pregnancy in aggravated herpes viral infection by histochemical method of pyridoxalphosphate dehydrogenase activity in placental plasmodiotrophoblast / 2503007

Histochemical method is used to assess pyridoxalphosphate dehydrogenase activity. The disturbance of transamination in placental plasmodiotrophoblast is shown by herpes virus antibody titre 1:12800 and decreased pyridoxalphosphate dehydrogenase activity below 12.5±0.37 standard units that is presented in the form of an increase of transaminase to 0.58±0.002 mmole/l in placental homogenate.

Differential diagnostic technique for haemorrhagic and ischemic strokes / 2503002

What is presented is differential diagnostic technique for acute haemorrhagic and ischemic strokes. The amount of low-molecular DNA fraction is specified by comparing 24-hour electrophoretogram from patient's blood plasma to the standard known concentrations. If the amount of low-molecular DNA 3 hours after the onset of acute stroke is 80-87 ng/ml, haemorrhage stroke is diagnosed. If the amount of low-molecular DNA 24 hours after the onset of acute stroke is 80-87 ng/ml, ischemic stroke is diagnosed.

Method of cultivation of oocysts of eimeria of cattle / 2503001

Method of cultivation of oocysts of eimeria of cattle, comprising microscopic examination of sporulation process, characterised in that the flotation solution is used, prepared from 1.5 kg of technical prilled ammonium nitrate and 1 litre of boiled water through which 3 g of feces examined are suspended in medical cups with 50 ml of the solution, the suspension is filtered through the metal mesh, exposed for 5 minutes, 12 ml of the upper layer is taken with the syringe without needle, transferred into the centrifuge tubes, centrifuged for 1 minute at 1500 rev/min, the surface film (1-2 drops) is removed with the wire loop and transferred to the degreased glass slide with an oval drawn with the wax pencil, 3-4 drops of tap water are added, the glass slides are placed on the support stand and then the support stand is placed in the desiccator, and then placed in the thermostat at a temperature of 25-28°C and then the microscopic examination is carried out daily for presence of sporulation processes.

Method for prediction of pregnancy / 2503000

Ability of the endothelium to activate fibrinolysis is assessed taking into account the fibrinolysis system health, and if the derived value is less than 11.0 units, sequential intermittent pneumatic compression is used. The method provides higher information value of extracorporeal fertilization (ECF) pregnancy; it is easy to use, informative; it requires no extra expensive equipment, reduces a drug-induced load on ECF females with reducing a probability of polypragmasy that makes it possible using it in practical healthcare.

Method for producing viable heterogenous skin cell population / 2502999

Invention provides a method for producing a heterogenous skin cell population implying the sampling of a skin biopsy material at a depth of 2 mm, tissue homogenisation in 0.9% aqueous sodium chloride at temperature +23 … +25°C, recovery of homogenate, filtration of the homogenate through an inert filter cloth with the pore diameter of 20 mcm, centrifugation of the homogenate at 400 g for 5 minutes at temperature +23 … +25 C.

Method for detecting t-lymphocyte hyperactivity / 2502998

Venous blood is sampled from persons being tested, and lymphocytes are recovered. A lymphocyte rosette formation level is determined in a thick-blood film of the lymphocytic suspension. If observing 5÷7% lymphocyte rosettes per 100% lymphoid cells, CD23+ and CD71+ hyperactivity is detected; observing 8÷10% lymphocyte rosettes shows CD23+, CD71+ and HLADR+ hyperactivity; observing more than 10% lymphocyte rosettes provides CD25+, CD23+, CD71+ and HLADR+ hyperactivity. Detecting the T-lymphocyte hyperactivity level is necessary for diagnosing autoimmune disease, delayed-type hypersensitivity test, tumour processes, rejection reaction, and postoperative complications.

/ 2244303

/ 2244513

/ 2244559

/ 2244927

/ 2244928

/ 2244929

/ 2244930

/ 2245548

/ 2245549

/ 2246110

FIELD: medicine.

SUBSTANCE: blood count is analysed, and a Calph-Caliph leucocytic intoxication index is calculated on the 3^rd post-fracture day. If the derived value is 1.8 and more, the following external fixation is expected to be accompanied by the development of an infectious inflammation of the wound.

EFFECT: using the declared method enables the early prediction of the infectious inflammation in the postoperative wound in the external fixation of the long bones.

1 tbl, 3 ex

The invention relates to medicine, namely to traumatology.

There is a method of detection of postoperative complications largest leukocytosis, leukocyte index of intoxication (LII) AA Calf-Caliph (nursing, 1941, No. 1, pp.31-36).

The well-known "Method for the diagnosis of complications in patients after angiosurgical after the reconstructive surgery" (Patent for invention №2083984 from 10.07.1997 year).

Known "Method of forecasting and detecting complications in the early postoperative period in surgical patients" (Patent for invention №2190216 from 27.09.2002 year).

As a prototype the selected method for predicting infectious and septic complications in severe trauma and blood loss based on the evaluation of LEAH in the first days after injury (Wroblewski OP, Isaev N, Adelia NV Prediction and prevention of infectious and septic complications in severe trauma and blood loss // Anaestheology and resuscitation. - 1988 - p.41-44).

However, the effectiveness of this method is not known in surgical treatment (plate osteosynthesis) patients with isolated fractures of long tubular bones.

The technical result of the proposed method is the ability to predict infectious inflammation in the surgical wound with plate osteosynthesis of fractures of long ribbed bones by RA the couple LEAH AA Calf-Khalifa at 3 days after fracture. When the value of 1.8 or more subsequent execution of the external fixations predict the infection and inflammation in the wound.

Surveyed 90 people. Of them: 30 patients operated on for fractures of long tubular bones with uncomplicated postoperative period - 2 group, 30 patients in whom after performing osteosynthesis developed suppuration of postoperative wounds (complications during the postoperative period) - 3 group. Control group-30 practically healthy people. All the subject performed a General analysis of blood and was calculated leukocyte index of intoxication AA Calf-Khalifa on 1, 2, 3, 4, 6, 8, 9, 11, 12 day from the date of injury.

LII=(4 m+3rd+2s+C)·(PC+1)/(mn+l)·(e+1),

where: m - plasmic order has been revealed;

Ju - young;

p - stab;

with - segmented;

PC - plasma cells;

l - lymphocytes, mn - monocytes;

e - eosinophils.

Surgical treatment was carried out on 6 - 9 days after fracture, patients were made of plate osteosynthesis. In the postoperative period was prescribed antibiotics (Cefazolin - 1 gr. intramuscularly 2 times a day for 5 days). The number of complications in surgical treatment of fractures of long tubular bones in the clinic is 2.6% (n=1154).

In the study of the obtained results is tov was established, that the magnitude of LII in patients with fractures of long tubular bones in groups in the first two days from the moment of injury is increased in 2 times in comparison with the control group (p<0.01) and was in the group with uncomplicated postoperative course 1 day - 2,85±in 0.288, 2 day - 2,67±0,434. In patients with complicated postoperative period 1 day - 2,41±0,268, 2 nights at 2.45±0,488. Over 3 days in patients with uncomplicated postoperative period index value decreased and did not differ from the values of the control group (table). Over 3 days in the group with complicated course index value was not changed and remained significantly high value relative to the control group (p<0.01) and the group with uncomplicated (p<0,01) (table). In the group of patients with a complicated course LEAH reached values of the control group on the 4 day study and amounted to 1.4±0,349 (p>0,05). Thus, if the magnitude LEAH is not reduced to 3 days before the boundary values of the control group, with subsequent surgical treatment in these patients developed infectious complications in the wound.

The analysis of the obtained material was established that the value of LEAH AA Calf-Khalifa at 3 days after fracture in the group with complicated postoperative period had values less than 1.8. We examined the control g is uppy and groups with uncomplicated postoperative period on the 3rd day of research value LEAH was less than 1.8 in all cases (table).

Examples:

1. Patient-Dom LN. 42 years. Case history No. 1206. He enrolled in the Department of trauma 05.04.2010 year with complaints of pain in the right forearm, violation of function of a limb. Injury caused on the street, fell hit area of the right forearm. Examination: General condition is satisfactory, somatically healthy. Locally: the axis of the right upper extremity right, no visible deformation. Determined by swelling in the middle third of the forearm, tenderness to palpation in the middle third of the ulna The pulsation of the arteries in the wrist distinct neurological disorders in the brush there. On radiographs is determined comminuted fracture of the middle third of the right elbow bone. Diagnosis: Closed comminuted fracture of the middle third of the right elbow bone with displacement of fragments. Upon receipt imposed a plaster of Paris splint is placed to the middle third of the shoulder, surgical treatment performed 11.04.2010, the elbow bone osteosynthesis plate LC-DCP. At 3 days after fracture LEAH had a value of 1.8. The patient in the postoperative period developed suppuration in the surgical wound, which required additional treatment at the Department of purulent surgery.

2. B-in VA 35 years. Case history No. 3060. He enrolled in the Department of trauma 03.12.2010 year with complaints of pain in the right tibia, the violation fu the functions of the leg. Injury caused on the street, fell sprained right foot. Examination: General condition is satisfactory, somatically healthy. Locally: the axis of the right lower limb is broken, there is a varus deformity in the lower third of the leg. Determined by swelling in the lower middle third of the leg, diffuse tenderness to palpation in the middle of the lower third of the right tibia. The pulsation of the arteries in the ankle distinct neurological disorders in the foot there. On radiographs is determined comminuted fracture of the middle of the lower third of the right tibia. Diagnosis: Closed comminuted fracture of the middle of the lower third of the right tibia with displacement of fragments. Upon receipt imposed system skeletal traction for the heel bone, surgical treatment performed 11.12.2010 year osteosynthesis of the tibia plate LC-DCP. At 3 days after fracture LEAH mattered 2,98. The patient in the postoperative period developed suppuration in the surgical wound, which required additional treatment at the Department of purulent surgery.

3. Ma Cove O.N. 33 years. Case history No. 1895. He enrolled in the Department of trauma 06.06.2010 year with complaints of pain in the right shoulder, violation of function of a limb. Injury caused on the street, fell, hit his right shoulder. Examination: General condition will satisfy the lnoe, somatically healthy. Locally: the axis of the right upper limb is broken, there is a varus deformity in the lower third of the shoulder. Determined by swelling in the lower and middle thirds of the shoulder, diffuse tenderness to palpation in the middle and lower third of the right shoulder. The pulsation of the arteries in the wrist distinct neurological disorders in the brush there. On radiographs is determined comminuted fracture of the middle of the lower third of the right humerus with displacement of fragments. Diagnosis: Closed comminuted fracture at the level of middle and lower third of the right shoulder with displacement of fragments. Upon receipt imposed system skeletal traction for olecranon, surgical treatment performed 14.06.2010 year - humerus osteosynthesis plate LC-DCP. At 3 days after fracture LEAH mattered 1,68. The postoperative course was unremarkable, the sutures were removed on the 12th day. 26.06.2010 was discharged for outpatient treatment in a satisfactory condition. Radiograph for evaluation of fracture consolidation was carried out 12.08.2010 year, there intermedial Union of the fracture. The patient was discharged to work.

1,38

Table
A method for predicting infectious inflammation of postoperative wounds in NAC the local osteosynthesis of long bones
Indicators LEAH AA Calf-Khalifa at 3 days after injury in patients with fractures of long bones
No.	1. The control group	2. Uncomplicated postoperative period	3. Complications during the postoperative period
1	0,88	1,24	3,25
2	0,99	1,64	2,24
3	0,62	1,58	4,5
4	1,58	0,82	3,2
5	0,63	1,12	1,94
6	0,38	0,91	2,12
7	1,37	0,91	1,80
8	0,6	3,4
9	0,56	1,4	3,85
10	0,99	1,61	2,98
11	1,25	1,75	1,83
12	1,68	1,68	3,38
13	0,63	1,45	2,01
14	of 1.34	1,54	1,98
15	1,42	1,48	1,85

16	1,56	0,78	2,3
17	1,66	0,88	3,88
18	the 1.44	1,11	1,92
19	1,38	1,65	2,01
20	1,26	1,68	1,83
21	1,74	1,45	was 2.76
22	1,72	1,68	1,85
23	1,64	1,23	1,82
24	0,44	1,56	3,24
25	1,42	1,45	1,94
26	1,58	1,64	1,84
27	1,68	1,69	1,99
28	the 1.44	1,72	2,05
29	1,37	1,46	1,9
30	1,6	1,54	1,89
M	1,23	1,40	2,45
±m	0,079	0,054	0,141
P		1/2>0,05	2/3<0,01; 1/3<0,01

A method for predicting infectious inflammation in the surgical wound with plate osteosynthesis of fractures of long tubular bones by analysis of the cellular composition of blood, thus determine the leukocyte index of intoxication (LII) AA Calf-Khalifa, characterized in that the parameters values leukocyte index of intoxication are calculated at 3 days after fracture, when the value of 1.8 or more, subsequent execution of the external fixations, predict the development of an infectious inflammation in the wound.