Method for production of fodder additive for prevention of animals and birds mycotoxicosis
FIELD: food industry.
SUBSTANCE: invention relates to biotechnology and fodder production, in particular, to a method for production of a fodder additive for prevention of anima and bird mycotoxicosis. The method envisages mechanochamical treatment of the raw stock components with further granulation of the produced mixture. The components are represented by a mixture containing a mineral sorbent, hydrolised lignin and fodder yeast cell walls. The mechanochemical treatment consists in milling individual components in rotary vortex mills with an embedded particle classifier of in the components mixing and treatment in the presence of at least one compound enzyme preparation affecting hydrolised lignin residual polysaccharides and yeast cell walls polysaccharides.
EFFECT: method allows to produce a compound additive having a wide range of effects with improved efficiency of fodder mycotoxins binding, stimulating the immune system and preventing malfunctions in the animal and bird digestive tract; at the same time, combined usage of hydrolised lignin, fodder yeast and a mineral sorbent as a preventive agent with animals allows to minimise the sorbent application side effect, recover the digestive tract homeostasis and normalise metabolic indices.
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The invention relates to biotechnology and forage production, in particular to the preparation of premixes and compound feeds containing special complex biological active additives, which prevent Ecotoxical animals and birds through effective binding of toxins, stimulate the immune system and prevent the development of disorders in the gastrointestinal tract.
Currently, there are various ways of obtaining feed additives aimed at the prevention of mycotoxicoses in animals and birds. The main method of decontamination of mycotoxin - neutralization using sorbents. Its effectiveness varies considerably because of the diversity of chemical structures and properties of mycotoxins, both sorbents. The essential parameters that determine the efficiency of binding of mycotoxins are specific complementarity of the surface of the sorbent to a certain class of mycotoxins, the surface area of the sorbent available for linking with mycotoxins, and the structure of macro-, meso - and microporous structure of the particles of the sorbent.
First, in practice, the composition of the feed began to apply mineral sorbents, then sorbents of organic origin.
For mineral sorbents square sorption is crucial, therefore, mineral sorbents D. who must be crushed. Natural mineral sorbents consistently crushed in crushers, ball, planetary and fibromalgia to achieve effective sorption capacity.
Known mineral polyfunctional enterosorbent on the basis of natural siliceous minerals, characterized in that as a natural siliceous mineral raw materials it contains schungite comprising mineral raw materials with a silicon dioxide content of 15.0-70,0 wt.%, and the average median particle size of enterosorbent is 15,0-10-6m (RF patent 2430731, published on 10.10.2011).
There is a description of the sorbent on the basis of silica-containing mineral raw materials containing sedimentary opal - chalcedony and cristobalite breed, containing not more than 15 wt.% clay minerals of the montmorillonite group and not more than 10 wt.% minerals of the group of micas (EN 2319488, 20.03.2008).
The above-mentioned mineral sorbents and other natural resources aluminosilicates (zeolites and bentonites), like most mineral sorbents preparations on their basis, effective only in large dosages: 2 to 5 kg/ton of feed. The effectiveness of mineral adsorbents against fumonisins and zearalenone can vary from high to low (S.J. Bursian, R.J. Aulerich, J.K. Cameron, N.K. Ames, B.A. Steficek. Efficacy of hydrated sodium calcium aluminosilicate in reducing the toxicity of dietary zearalenone o mink. J. Appl. Toxicol., 1992, 12, pp.85-90). Against ochratoxins efficiency is also small (W.E. Huff, L.F. Kubena, R.B. Harvey, T.D. Phillips. Efficacy of hydrated sodium calcium aluminosilicate to reduce the individual and combined toxicity of aflatoxin on and ochratoxin A. Poult. Sci., 1992, 71, pp.64-69). In relation to toxin T-2 efficiency of mineral sorbents only conditional (L.F. Kubena, R.B. Harvey, W.E Huff, D.E. Corrier, T.D. Phillips, G.E. Rottinghaus. Efficacy of a hydrated sodium calcium aluminosilicate to reduce the toxicity of aflatoxin on and T-2 toxin. Poult. Sci., 1990, 69, pp.1078-1086), and against trichloroethanol, such as vomitoxin and nivalenol close to zero (R. Patterson, L.G. Young. Efficacy of hydrated sodium calcium aluminosilicates, screening and dilution in reducing the effects of mold contaminated corn in pigs. Can. J. Anim. Sci., 1993, 73, pp.615-624 // L.F. Kubena, R.B. Harvey, R.H. Bailey, S.A. Buckley, G.E. Rottinghaus. Effects of hydrated sodium calcium aluminosilicate T-Bind™ on mycotoxicosis in young broiler chickens. Poult. Sci., 1998, 77, pp.1502-1509).
In feeding practices in the Russian Federation is widely used by Russian holistic medicine "Fungistatic". From the description of the patent of the Russian Federation No. 2420565 (published on 10.06.2011) is known a method of producing a biological product "Fungistatic" for the removal of mycotoxins from food and feed raw materials, which involves mixing in a ratio of 5:1 sorbent selected from silicates, and sorbent selected from the layered sorbents, the connection of the mixture with Riboxin, lecithin, L-carnitine, organic acid selected from the group comprising succinic acid and propionic acid, or its salt, a mixture of oligom is ctazy and inulin (30:70) and proteolytic enzyme preparation on the basis of the culture fluid of Bacillus bacteria subtilis "Protocontinent", moreover, mix the components taken in the following amounts (g/kg of the final product):
Riboxin - 15-20;
lecithin - 20-30;
L-carnitine - 15-20;
the organic acid is succinic or propionic acid or their salts - 10-15;
a mixture of oligofructose and inulin 30:70 - 30-50;
enzyme proteolytic product of "It" - 25-30.
A method of producing a biological product "Fungistatic" as follows. In the apparatus with stirrer mixed sorbent, representing a zeolite or bentonite, and the sorbent, representing vermiculite or montmorillonite. The mass ratio of the mixed sorbents 5:1, respectively. At this time in a different unit mix Riboxin (30 g), L-carnitine (20 grams), salt of organic acid - sodium succinate (15 g), oligofructose mixed with inulin (30:70) (50 g), "It" (30 g) and mix thoroughly. The resulting mixture was added to a mixture of sorbents (835 g). Get the drug which includes the number of components correspond to 1 kg of the finished product. When developing a drug "Fungistatic" the authors proceeded from the assumption that if the composition of the drug in addition to the sorbents will be the substance that protects the hepatocytes of the liver, as well as a stimulating exchange reactions in hepatocytes and their recovery, the effect of neutralization of toxins should be much higher. This mechanism of action is I these substances must be different. Components of a biological product "Fungistatic" according to the authors affect the utilization of mycotoxins in the animal or human body, allow to secure the consumption of contaminated raw materials, to neutralize the harmful effects of mycotoxins.
The sorbents of the second generation is produced from the biomass of yeast and bacteria, while the active component is contained in the cell wall of microorganisms. Such sorbents do not damage the intestine and their sorption ability in 2-2,5 times higher than that of mineral sorbents first generation. However, such a high efficiency is manifested only in respect of certain mycotoxins: hydrophobic mycotoxins sorbents second generation almost not inactivate (G. Schatzmayr, W.-D. Moll, U. Hofstetter, E. Vekiru, D. Schatzmayr, Y.H. Cheng. Mycotoxin deactivating feed additives in animal nutrition. BOKU-Symposium Tiererhahrung, 02 Nov. 2006, Wien, pp.47-53 // US Patent 6045834. Compositions and methods for removal of mycotoxins from animal feed. 2000 // M.V.L.N. Raju, G. Devegowda. Influence of modified mannan oligo-saccharides on broilers exposed to individual and combined mycotoxicoses of aflatoxin on, ochratoxin and T-2 toxin. Abstract No. 229, PSA'99 University of Arkansas, Springdale, August 8-11, 1999, p.52).
A typical representative of such sorbents can be considered the drug of Mycosorb company Alltech, U.S. patent 6045834 (US Patent 6,045,834. Compositions and methods for removal of mycotoxins from animal feed. 2000). The patent States that the cell wall of yeast and mineral clay, acting in synergy, provide the op is kalinoe binding of mycotoxins. The patent shows through in vitro experiments that aflatoxin, zearalenone and fumonisin well-linked sorbent type Mycosorb from aqueous suspensions of food, but vomitoxin, ochratoxin and T-2 still remain in the solution at 67-87% of the initial concentration.
It is established that the cell wall of yeast due to specific complementarity of the inner surface to a certain class of mycotoxins absorbs them more efficiently than intact yeast cells. A method of obtaining a feed additive, in which raw materials based on plant, animal and/or microbial product containing oligosaccharides and/or polysaccharides, are treated in such a way that the structure of the cell wall is opened, and the number of free oligosaccharides and/or polysaccharides on the surface of the cell wall increases (RF patent No. 2196437, the date of publication of the application 10.06.2001). Food includes this ingredient in an amount of 0.1-2 wt.%. Feed additive, obtained by this method is effective to prevent stomach and intestinal diseases, inhibiting the growth of harmful microbes. Introduction to food additives such allows you to stop the use of antibiotics, which has a positive impact on animal health.
In the patent of Russian Federation №2406516 (published 29.12.2010) described the method of producing drug for prevention is Pecci digestive tract in farm animals and poultry, characterized in that it includes a preliminary mixing of the yeast biomass with an enzyme complex with depolymerase activity towards β-glucan. The resulting mixture is subjected to mechanical activation activators planetary, or vibrating or vibratory centrifugal types, or roller mills, providing faster grinding media 60-400 m2/s and the residence time in the treatment zone of 0.5-10 min, after mechanical activation of the intermediate is subjected to pelletizing and warming at the optimum temperature steps of the enzymatic complex.
It is known that the cell wall of plants can also be effective enterosorbent. For example, the shell pine nuts can be used for the prevention of gastrointestinal disease of young cattle. The invention consists in the application shell pine nuts into a fine powder as a means of preventive therapy of gastrointestinal diseases of calves. Use reduces the incidence and improve the safety of young cattle (RF patent No. 2242239 published 20.12.2004).
Processing waste wood can be used as a feed additive. There is a method of cooking food, including introduction to OS the ESD diet granulated mixture of waste wood with a binder, characterized in that, with the aim of improving the quality of food as waste wood use pre-dried to a moisture content of 12% by mass, zero fiber, containing less than 10% of lignin, less than 5% extractives, and less than 12% ash matter, less than 0.1-12 mg/kg heavy metals as a binder used molasses, Ca-lignosulfonate and/or bentonite in the amount of 8.0 to 20.0% by weight of the feed and introducing the resulting mixture into the main diet in the amount of 10-25% (RF patent No. 2027376 published 27.01.1995).
Known drug "Pectoris" for the prevention and treatment of gastrointestinal diseases of young farm animals (RF patent 2141329 published 20.11.1999). The product containing pectin (40-90% of the dry weight of the preparation) and ligninases complex (cellulose, hemicellulose, lignin, monosaccharides - 10-60%), obtained from the bark of coniferous trees and deciduous, citrus and other plant materials using acid hydrolysis (as the USSR №1165684 published 07.07.1985).
Lignin as a feed additive used for a long time. For example, patent USSR No. 1831295 (published 30.08.93) was suggested as components in the feed mineral Supplement use of feed grains from Phyllophora, which additionally contains zinc; manganese, copper, cobalt and forage lignin in a predetermined ratio to the components. The content of lignin in forage additive was 44-56%, and in the finished feed 1-2%. The use of forage lignin due to its high adsorption capacity due to a developed specific surface area (700-800 m2/g), which means that it absorbs and holds on to its highly developed surface of pathogenic bacteria and toxins in the digestive tract of animals and birds. In addition, according to the authors of the patent forage lignin is a good antioxidant and ' buffer ' between incompatible macro - and trace elements feed additives, resulting in increases their shelf life and improve their quality. The disadvantage of this invention is the lack of production of forage lignin". Lignin, which is a large waste of hydrolysis industry cannot be used as forage lignin because of its high toxicity, which is caused by residual quantity of inorganic acids and toxic degradation products of sugars: furfural, methylpyrrole, oxymethylphenyl and other low molecular weight products of acid hydrolysis of wood.
Processed with alkali, neutralized and washed lignin is widely used in medicine. The number of known methods of obtaining medical enterosorbent on cos the ve hydrolytic lignin (CH) (A.S. The USSR №556811, 1977; A.S. USSR №803157, 1980; RF Patent №919186, 1981; RF Patent №1290583, 1985; RF Patent №2026078, 1991; RF Patent №2116080, 1997). The basis of all known methods of obtaining medical enterosorbent of hydrolytic lignin is the treatment of the latter with alkali solution with the subsequent operations of washing, neutralization, grinding in aqueous slurries, dewatering them to different water content by filtering, centrifugation, and drying, with a drug - enterosorbent in different dosage forms: in the form of a powder with a content of dry particles of lignin from 20 to 100%, videospersonal paste containing 14-20% of dry particles of lignin, and in the form of an aqueous suspension with a solid content of 8-10%to 13%. The resulting preparation in the form of various dosage forms are subjected to sterilization one of the known methods: steam, heat, radiation. Additional bactericidal properties of the chelators containing lignin, you can make processing intermediates solution of iodine (RF patent No. 2302866 published 20.07.2007) or silver (EAPO application No. 200900003 published 30.10.2009).
I received these methods medical lignin low adsorption capacity. To implement these method of obtaining medical lignin requires large expenditures of heat and electricity, and ha is Acterna loss drug, associated with the prescribed technology stages of drying the semi-finished product, and grinding the dried product.
RF patent №2026078 (published 09.01.1995) describes a method of obtaining sorbent by processing hydrolytic lignin alkali leaching of alkali, neutralizing and grinding the suspension containing by weight 8-13% of dry particles of lignin obtained suspension containing by weight 8-13% of dry particles of lignin thicken up the content by weight of 14-20% of dry particles of lignin and disintegrate to the size of the particles of lignin in her 0.005 to 0.25 mm to produce a homogeneous paste. The high content more crushed particles of lignin increases the sorption capacity and provides greater therapeutic effect of the drug. The claimed range of grinding particles of lignin from 0.005 to 0.25 mm with additional getintegervalue suspension due to the fact that with less grinding particles (less than 0.005 mm) notes the inclusion of particles of sorbent in the intestinal wall. This factor has been proven in the study by electron microscopy of the small intestine and is regarded as unfavorable.
It is also known that attempts to obtain a solid dosage form hydrolytic lignin was accompanied by a decrease in the adsorption activity during drying [Enterosorption. / Ed. by N.A. Belyakova. - L.: Center sorption technologies, 1991, - 336 S.].
Despite almacenadora, "lignin" in the form of a powder with fractional composition mass fraction of particles of size less than 0.5 mm - not less than 90%, 0.5-2 mm - no more than 5%, or lignin hydrolysis in aqueous suspension is widespread in medical and veterinary practice called "Polifan" (patent of the Russian Federation 2297214 published 20.04.2007).
It should be noted that in reality "Polifan" - enterosorbent, representing a pH-neutral chopped wood residue after acid hydrolysis (lignin), cleaned from impurities and activated by the alkaline processing at elevated temperature followed by washing from alkali and grinding. In his composition "Paliparan contains 34-37% lignin, 60% of hydrocellulose and 1-3% of mineral substances. In the gastrointestinal tract, the drug absorbs, holds and displays the body of pathogens (E. coli, Salmonella, Proteus, Staphylococcus, yeast microflora, Pseudomonas aeruginosa), they secrete toxins and heavy metals, nitrites, nitrates, too much of some products of metabolism, causing the development of endogenous toxicosis. The drug has a positive effect on intestinal peristalsis and has an antioxidant effect. "Polifan" veterinary, having a wide range of functional groups (metaxylene,carboxyl, hydroxyl), causing the possibility of chemisorption and complexation, exhibits high sorption activity and non-specific detoxification effect. It does not have a large specific surface area (up to 15-20 m2/g), as due to chemical processing in the manufacturing process of medical lignin decreases the natural porosity of natural lignin of the wood (Repnikov E.A., Aleshin L.A., Glazkova SV, Fofanov A.D. Radiographic studies of hydrolytic lignins // New advances in chemistry and chemical technology of vegetable raw materials: proceedings of the II all-Russian conference, April 21-22, 2005, Barnaul, 2005. CN P.87-90).
Medical lignin is a comprehensive basis of chelators in combination with prebiotics (RF patent No. 2167669 published 27.05.2001), with other sorbents (RF patent No. 2234931 published 27.08.2004), with prebiotics and other sorbents (RF patent No. 2433751, published on 20.11.2011).
For the purposes of veterinary medicine are similar to complex drugs on the basis of "Politeama", for example lignin with prebiotics. In the patent of Russian Federation №2440121 (published on 20.01.2012) disclosed veterinary pharmaceutical composition for the treatment of diseases of the gastrointestinal tract and intoxications of various etiologies in animals, containing as active ingredients the hydrolysis of the initial lignin and the prebiotic group: lactulose, fructo-oligosaccharides, galactosamine, inulin, made in the form, suitable for use in the composition of the mixture with the feed, when the content of lignin in an amount of from 30 to 95 wt.%, and prebiotic from 5 to 50 wt.%.
In addition, there is a method of sorbent, which comprises grinding the seed husk, acid hydrolysis extraction of water-soluble dietary fibers and the formation of a specific structure consisting of lignin, cellulose and melanin, where acid hydrolysis carried out with 0.1-36% acid solution within 0.3 to 4.5 hours boiling under pressure in the range of 0.1 to 0.7 MPa, washing with water and/or 0.1 to 1.0% of an alkaline solution and then softened water, followed by drying of the product (application EA 200900003 A1, published 30.10.2009). The adsorbent is a porous multi-level matrix on the basis of lignin, cellulose and melanin with integral porosity (0,04-50) microns. Sorbent capable of adsorbing such hazardous chemicals as pesticides, mycotoxins, polychlorinated biphenyls, viruses, pathogenic bacteria and complex hydrocarbons. This effect is ensured not only by the presence on the surface of the chemically active centers and biologically active ingredients, but layered and porous structure of the sorbent with a multimodal distribution of pores (micro-, meso-, macro-)that allows you to absorb mole is uly various sizes, geometry and chemical nature.
In fodder for the prevention of mycotoxicosis and gastrointestinal diseases chelators are used in the composition of feed additives. The objective of the economic justification of prophylactic use of chelators with a low level of contamination of feed with toxic substances (including mycotoxins) in the composition of feed additives along with one or more chelators injected nutrients, amino acids, vitamins, enzymes, coenzymes, prebiotics, probiotics, hepatoprotectors, immunomodulating agents, emulsifiers, organic podnikatel and other biologically active substances have a positive influence on the productivity of agricultural animals and birds. This approach is quite justified, as most modern scholars believe that safe levels of mycotoxins in feed and food products does not exist.
In feed also use soft methods of processing of natural plant materials to enhance its sorption properties and conservation in the feed additive composition of biologically active substances.
In the patent of Russian Federation №2433738 (published on 20.11.2011) described the invention, intended for use in the production of feed for livestock and poultry. As the cellulite, tighten soderzhaschego substrate using sugar beet pulp or waste production of juices and wines - Marc, who is crushed to the size of the particles with a particle size characterized by the remains on the sieve 2.0 to no more than 15%, add prior autolysate of yeast Saccharomyces cerevisiae in the amount of 2.5-8.5 wt.% per 1 ton of dry product and process water to a moisture content of 18-47%, are pasteurized mixture at a temperature of 80°C for 2-2,5 h, cooling to a temperature of 48-50°C, followed by enzymatic processing of the substrate cellulolyticus enzyme for 2.5-4 hours at a temperature of 48-50°C, then dried to a moisture content of 8-12% at a temperature not exceeding 85°C and to the resulting mixture add 15-45 wt.% probiotic feed additives for farm animals Farm KM. The latter is obtained by separate submerged cultivation of strains of Bacillus subtilis VKPM B-8130, Bacillus subtilis VKPM B-2984, Bacillus subtilis VKPM B-4099 and Bacillus licheniformis In PMBC-4162 and subsequent solid-phase cultivation liquid cultures of Bacillus subtilis on a mixture of beet pulp and wheat bran, dried to a moisture content of 8-10%. Moreover, a liquid culture of Bacillus licheniformis In PMBC-4162 directly mixed with sugar beet pulp and dried. After that dried cultures are mixed and subjected to grinding to obtain a homogeneous product. The invention provides for increasing the activity of the deactivation of many mycotoxins, increases the productivity of animals and the tics by increasing the digestibility of feed, increase feed conversion by 10% and enhance metabolic processes in the body, enhances the safety of the population.
An overview of known methods and materials for effective feed additives - sorbent mycotoxins can make some General conclusions.
The disadvantages of the known methods are limited sorption capacity of sorbents with respect to several classes of mycotoxins, the complexity and high cost of production. Developed effective method of processing lignocellulosic plant raw material, allowing to realize sufficient natural ability of plant cell walls to bind a wide class of mycotoxins due to specific interaction of the latter with the polymers present in the cell wall, as well as with the surface of microporous structures formed by these polymers in vivo development of plants.
The closest technical solution is the way to obtain a premix comprising grinding plant, mechanochemical processing of the mixture of plant material, abrasive material and natural sorbent in the high-stressed actuators planetary, vibration and vibratory centrifugal type, with mechanochemical processing is carried out by grinding the mixture of plant material, and rasinovo material and natural sorbent, taken in the ratio of 40-85, 5-40, 10-20, respectively, when the acceleration of milling bodies 60-400 m/s2and processing time of 0.5 to 10 minutes (RF patent 2366268 published 10.09.2009). The machining conditions are determined by the kinetics of the grinding and form a comminuted material. When the acceleration of the grinding media (balls) of less than 60 m/s2not provided by the introduction of particles of amorphous silica in the particles of plant material, which reduces the efficiency of dissolving the contents of the cells upon contact of the drug with water environments. When the acceleration of 400 m/s2leads to heating of the processed mixture, which can lead to the destruction of biologically active substances. Application processing less than 0.5 minutes does not provide the necessary grinding and almost never leads to the formation of oligosaccharides, the increase in processing time over 10 minutes can lead to mechanochemically target component.
The disadvantage of this method is the high energy consumption of grinding in planetary mills, vibratory and vibratory centrifugal types, as well as the inability to achieve the objectives set for certain types of crushed raw non-optimal composition of the sorbent does not allow to achieve the stated parameters of adsorption of mycotoxins.
Technical task patentable invention is to develop a method of obtaining the effect of the main feed additive for the prevention of diseases of the gastrointestinal tract and intoxications of various etiologies in animals and birds.
The technical result, which provides the solution of the problem of the invention is obtained by the method of complex Supplement with a broad spectrum of activity, with increased efficiency of binding of mycotoxins from feed and stimulate the immune system and prevent disturbances in the gastrointestinal tract of animals and birds. At the same time sharing micronized lignin, fodder yeast, mineral sorbent as a means to prevent animals allows to obtain the result as a complex of positive effects: minimizing adverse effects from the use of the sorbent, the restoration of the homeostasis of the gastrointestinal tract, normalization of rate of metabolism, which ultimately leads to quality improvement of zootechnical and economic performance of livestock.
The claimed technical result is achieved due to the implementation of the method of producing a feed additive for the prevention of mycotoxicoses in animals and birds, consisting of mechanochemical treatment of the mixture components with subsequent microgranulate mixture obtained according to patent the solution as a mixture of components a mixture of at least one mineral sorbent, hydrolytic lignin and cell article the NCI fodder yeast, this mechanochemical treatment consists in grinding the mixture components and their treatment in the presence of at least one complex enzyme preparation, affecting residual polysaccharides hydrolysis of lignin and polysaccharides of the cell wall of yeast, followed by microgranulation the resulting milled mixture in the fluidized bed apparatus.
Grinding components can be done as a mixture and each component separately with their subsequent mixing.
The feed mixture contains the sorbent in the amount of 5-30 wt.%, lignin in the amount of 30-60% of the mass. and the cell wall of yeasts in the amount of 5-50 wt. -%
As a complex enzyme preparation can be applied dry enzyme preparation of Xiamen based on the fungal strain Trichoderma longibrachiatum.
Grinding is desirable to carry on the mill rotor-vortex type with built-in classifier particles at a temperature of 40-85°C, the size of the crushed particles must be equal 50-250 microns.
Microgranuloma carried out in the dryer-granulator pseudocapsule layer, a rotary granulator, disc granulator or the granulator-mixer with high shear.
In the process of microgranulate spend additional chemical activation of hydrolytic lignin and glue the internal wall of yeasts using, at least one of the complex enzyme preparation, affecting residual polysaccharides hydrolysis of lignin and polysaccharides of the cell wall of yeast, this complex enzyme preparation used enzyme preparation of Xiamen based on the fungal strain Trichoderma longibrachiatum.
As biologically active substances, which are distributed to the diet of animals and birds, use lecithin in the amount of 10 to 40 g per 1 kg of the mixture components, Riboxin in the amount of 10-20 g per 1 kg of mixture components L-carnitine in the amount of 10-20 g per 1 kg of the mixture components, as well as podnikatel, inorganic or organic selenium compounds.
At the stage of microgranulate may be added, at least one binding agent, which is used as sucrose and/or 5% aqueous solution of carboxymethylcellulose.
As hydrolytic lignin is desirable to use lignin, rested in bed in the dumps 10-15 years with humidity from 5 to 25%, as a mineral sorbent - shungite clinoptilolite, vermiculite, zeolite and/or montmorillonite.
The invention in part of the method of producing a feed additive for the prevention of mycotoxicoses in animals and birds is separate and/or joint grinding of lignin, zeolite and fodder yeast, mixing crushed is komponentov with add, at least one binding agent and in microgranulate mixture in the fluidized bed apparatus with a fodder additive.
Received feed additive contains as active ingredients micronized lignin, micronized fodder yeast and micronized mineral sorbent selected from the group effective sorbents - silicates: shungite clinoptilolite, vermiculite or montmorillonite, made in the microgranular form, suitable for use in the composition of the mixture with premixes and compound feed, when the content of lignin in an amount of from 30 to 60 wt.%, yeast is from 5 to 50 wt.% and mineral sorbent from 5 to 30 wt.%.
Feeding animals and poultry feed additives is carried out in a mixture with feed at a dose of 1 to 2 kg of the drug per 1 ton of feed, irrespective of the content of mycotoxins in the feed.
The stated feed additive is designed for implementation with the trade name "Nordex".
As shown by tests, the composition of the obtained feed additives can effectively adsorb, both polar and hydrophobic mycotoxins. At the dosage of 1-2 kg/ton of feed additive "Nordex" should be not less than 50%:
- DON at concentrations up to 5 mg/kg of feed;
- T-2 in concentrations up to 5 mg/kg of feed;
- ochratoxins in concentrations up to 1 mg/kg of the Orme;
- zearalenone in concentrations up to 2 mg/kg of feed;
- aflatoxin in concentrations up to 0.2 mg/kg of feed;
- fumonisins in concentrations up to 5 mg/kg feed.
The achievement of the claimed technical result is achieved due to the following distinctive features:
1. Use as a mineral component of the sorbent types of silicate is montmorillonite (synonym - morenet). Structure of the mineral resembles a three-layer package: two layers of si tetrahedra, facing peaks to each other, with the two sides of the cover layer alyumogidroksidnykh octahedra. In this regard, the relationship between packages weak, packet length is great and it can get ions and water molecules. Because of this mineral at wetting strongly swells. The presence of isomorphic substitutions, large specific surface area (up to 600-800 m2/g) and the ease of penetration of the ions in the packet space causes a significant cation exchange capacity (80-150 mmol·EQ/100 g). The structure of the particles and crystals of montmorillonite clay has the least traumatic effect on the mucous membrane of the gastrointestinal tract.
2. Nonspecific sorption capacity of natural morenita already high enough, can be improved through various types of processing, which increases the distance between mikroC stirami mineral. Micromelia of morenita and mechanochemical activation are proposed on the mill rotor-vortex type.
3. As the second component of the sorbent and as a source of immunomodulators and other biologically active substances use dry fodder yeast, namely the cell wall of yeast, which adsorb mycotoxins better than the biomass of yeast. The effectiveness of yeast glucans for fattening pigs is higher than the efficiency of just the cell wall of yeast. In turn, the efficiency of the cell wall of yeast is higher than that of intact yeast cells.
4. It is proposed to use the simplified technology for the processing of dry biomass of yeast in order to obtain a dry mixture of biologically active fractions, which will be used in the developed feed additives.
The traditional process of separating the components of the yeast biomass is expensive, because it includes the technological stage of extraction, centrifugation, concentration and drying. At each technological stage there is a risk of microbial contamination, which complicates the technology and increases the cost of processes and devices. Consumes large amounts of water and energy.
The simplification of the technology is that to increase the sorption capacity of yeasts and bi is the availability of biologically active components of the yeast biomass dry fodder yeast microsolar on vortex mill-classifier, including together with mineral sorbent and complex enzyme preparation, acting on polysaccharides of the cell wall of yeast. The method of opening the cell wall of yeast differs from the known methods (patent RF №2196437, the date of publication of the application 10.06.2001 RF patent No. 2406516 published 29.12.2010) the fact that the vortex mill-classifier capable of providing acceleration samoizolacija particles of raw materials 400-800 m2/s without significant overheating due to short processing time (less than a minute) and due to the design of the mill classifier, the milling chamber which is equipped with external water jacket.
4. A distinctive feature of the developed feed additives will be used in its composition as the third component of the sorbent budget rested in bed in storage hydrolytic lignin.
It is known that lignin (TU 64-11-05-87) is a waste of hydrolysis industry: production of glucose, industrial alcohol and acetone from wood as a result of its processing with concentrated sulphuric acid (hydrolysis). In the period from 1930 to 1990 in Russia were 12 plants such profile, currently the only active one is the plant on the territory of the Kirov region. Lignin were removed from industrial enterprises in special the open platform for its storage (dumps). Currently, the amount of hydrolytic lignin in composition of such dumps in the country is estimated at 70-90 million tons. The largest dumps are located in the Krasnoyarsk region (15 million tons), Irkutsk oblast (20 million tons), Sverdlovsk region (15 million tons), Kirov region (10 million tons). Some dumps are owned by the municipalities (blade hydrolytic lignin in the Kostroma region, Kan blade), part - industrial enterprises (Kirov blade), the rest are abandoned.
For long time storage dump occurred the natural destruction of the impurities in the composition of hydrolytic lignin and its leaching precipitation, as a result of this disappeared its acidity and toxicity. At the same time preserving the natural ordered structure and porosity of Patrignano, restored natural sorption properties of its surface. The lignin content in the depth of the blade during long-term storage increases by more than 10%, ash content of lignin increases from 0.5% to 10%, the content of extractives is reduced by 25%, and the content of residual polysaccharides decreases in 20 times (S.M. Circles, IV Sumarski, A.V. Pranovich, YU.N.. Sazanov, MA Zarubin. The study of lignin from dumps Arkhangelsk hydrolysis plant. // Physical chemistry of plant polymers: materials of the IV International conference / edited by Dr. of chemistry, Professor KG Bogolitsyn. - Arkhangelsk, 2011. // C-185).
Thus, lignin, rested in bed for 10-15 years in the dumps, passed the natural slow transformation, perhaps even slow solid-phase fermentation involving a limited number of microorganisms able to use the residual cellulose and extractive lignohumic substances as nutrients in the initially unfavorable conditions for intensive growth of the microbial biomass.
Rested in bed lignin is epileptology a lot with humidity of 65-70%, prone to spontaneous combustion in the dumps. Rested in bed in the dumps at least 10-15 years lignin well zombiroval non-polar mycotoxins. Before microasperities lignin is dried to a moisture content of 5-25%, for example at the dryer as-4, the production company "SP-co" (Pskov).
5. Micromelia fibrous particles hydrolytic lignin on vortex mill-classifier can improve the efficiency of adsorption of mycotoxins, including possible chopping together with mineral sorbent and/or dry fodder yeast and/or complex enzyme preparation influencing residual polysaccharides hydrolysis of lignin and polysaccharides of the cell wall of yeast. This destroys the macroporous structure of lignin, which PR is chicosci not involved in the sorption process, preserves the structure of meso - and micropores. The active surface of meso - and micropores become more accessible to molecules of toxins, the transport speed of toxins from the surface of the mesopores in the micropore volume increases. The binding of toxins microtelecom lignin in the laboratory experiments was increased to 30% compared to the dried fibrous hydrolytic lignin.
The process of micromelia carried out at the optimum temperature for the action of hydrolytic enzymes 40-55°C, preventing temperature increase grinding above 85°C at which there is a change in porosity and a decrease in the sorption capacity of lignin due to the collapse of the mesopores. The melting temperature of lignin to about 90°C.
6. To improve consumer properties, ease of storage and transportation, reduce fire risk, improve sanitary and hygienic conditions of production and use, as well as to prevent the increase in the content of pulverulent fraction in feed microtrenching feed additive microgranular. Microgranuloma is carried out at a product temperature not higher than 60°C using dryers-granulators pseudocapsule layer, rotary granulators, disc granulator, granulator-mixer with high shear.
7. With the aim of improving the effectiveness of cor the new supplements are offered at the stage of microgranulate to enter into the composition of known biologically active substances, which, as a rule, were distributed to the diet of farm animals and birds. For the prevention of mycotoxicoses in the composition of the feed additive is introduced as hepatic lecithin in the amount of 10 to 40 g per kg of the additive, Riboxin and L-carnitine 10-20 g per kg of the additive. In accordance with the design standards for 1 kg of finished feed and the recommended dose of feed supplements for prevention of gastrointestinal infections enter podnikatel (organic acids and/or their salts), and as an immunomodulator - inorganic or organic selenium compounds.
The data given in Examples 1-3 describe, but do not exhaust the possibilities of the proposed method and develop food additives "Nordex".
The speed of rotation of the rotor vortex mill set equal to 4000-5000 rpm, and then using a screw conveyor produce supply air dry hydrolytic lignin from the blade of the Kirov biochemical plant in the grinding chamber rotary vortex mill with integrated classifier particles. The temperature inside the grinding chamber is 50°C. After micromilieu particle dimension of 50-250 μm arrive at the stage of mixing the components.
The speed of rotation of the rotor vortex mill set equal 4000-7000 rpm, and then using a screw conveyor p is izvodyat flow biomass feed yeast (humidity not more than 10%) of the grinding chamber rotary vortex mill with integrated classifier particles. In the grinding process is the destruction of the cell wall of yeast due to mechanical impact. The temperature inside the grinding chamber is 50-65°C. After grinding, the particle dimension of 50-250 μm arrive at the stage of mixing the components.
The speed of rotation of the rotor vortex mill set equal 4000-6500 rpm, and then using a screw conveyor to produce a flow of zeolite deposits in the vicinity of the city of Gubkin, Belgorod region (humidity not more than 10%) of the grinding chamber rotary vortex mill with integrated classifier particles. In the process of micromelia happens mechanochemical activation of natural aluminosilicate. The temperature inside the grinding chamber is 70-80 C. After grinding, the particle dimension of 50-250 μm arrive at the stage of mixing the components.
Mikrosmeshcheniya components with a particle size of 50-250 μm and sucrose as a binder agent is loaded into the drying apparatus of the fluidized bed in the following proportions:
|microecology lignin||400 g|
|mikrosmeshcheniya fodder yeast||300 g|
|mechanically activated zeolite||100 g||sucrose||150 g|
As an additional bonding agent supplied through the injector uses a 5% aqueous solution of carboxymethylcellulose.
The process of granulation-drying was carried out on pilot plant collected on the base of the stand Ventilus 2.5 firm Innojet (Germany) under the following conditions:
|air consumption||20 m3/kg/hour|
|the feed rate of the binder|
|(5% solution of carboxymethyl cellulose)||3 g/min|
After completion of the process of granulation-drying the granule size is 200-800 μm, residual moisture of dry granules not more than 6%.
In comparison with analogues obtained in this way variant of the feed additive in vitro has a high sorption capacity with respect to mycotoxins (table 1).
|Comparative sorption of mycotoxins|
|Ochratoxin a (OTA)||61%||59%||43%|
To confirm the effectiveness of the developed food additives "Nordex" were tested in vivo in broilers using feed, balanced nutritional standards in accordance with the recommendations of wildebeest breeding of the RAAS, and contaminated with mycotoxins.
The experiment was carried out in the experimental farm breeding on the chicken cross the Cobb avian 48 with daily up to 3 days of age under the scheme, presented in table 2.
|The scheme of experiment on the broiler|
|Group||Characteristics of feeding|
|1 period||2nd period|
|1. The positive control (PC)||Complete feed, balanced nutrition without supplements sorbents(PC)||Complete feed, balanced nutrition without supplements sorbents (PC)|
|2. The negative control for PTA||PC with PTA (ready aft, 40 parts feed accounts for part 1 of pollutant)||PC with PTA (ready aft, 20 parts feed accounts for part 1 of pollutant)|
|3. PC + Nordex-1 kg||PC + Nordex (1 kg/t feed)||PC + Nordex (1 kg/t feed)|
|4. PC + Nordex-2 kg||PC + Nordex (2 kg/ton feed)||PC + Nordex (2 kg/ton feed)|
|5. PTA + Nordex-1 to the||PC PTA + Nordex (1 kg/t feed)||PC PTA + Nordex (1 kg/t feed)|
|6. PTA + Nordex-2 kg||PC PTA + Nordex (2 kg/ton feed)||PC PTA + Nordex (2 kg/ton feed)|
|7. PTA + Fungistatic||PC PTA + Fungistatic (2 kg/ton feed)||PC PTA + Fungistatic (2 kg/ton feed)|
|8. PTA + Mycosorb||PC PTA + Mycosorb (2 kg/ton feed)||PC PTA + Mycosorb (2 kg/ton feed)|
The results of the analysis of feed for broilers on the presence of mycotoxins are presented in table 3.
|Analysis of the feed for the presence of mycotoxins|
|1||2||3||1 period||2nd period|
|T2toxin||to 0.032||0,035||0,028||0,059||0,067||0,046||to 0.032|
|Zearalenone||not OBN.||0,188||0,042||0,011||0,007||traces||not OBN.|
|Fumonisin||not OBN.||not OBN.||not OBN.||0,93||1,18||0,38||0,33|
From the table it is seen that the feed of the negative control group in the first cultivation period contained more T2toxin and fumonisin. The difference with the control of these mycotoxins was on 13,55 and 26,88%. Feed the negative control of the second cultivation period contained almost 2 times more ochratoxins, and its concentration was almost 7 times higher than the MCL (0.01 mg/kg).
In the experience for broilers considered basic zootechnical performances: live weight twice for the experience by individual weighing of all livestock, livestock safety, consumption and cost of feed per 1 head and 1 kg of growth. In carrying experiments determined the digestibility and utilization of nutrients of food. At slaughter the birds were determined the absolute and relative weight of liver, kidneys and spleen. In the liver was determined by the content of vitamins A, E and B2.
To confirm the effectiveness of the developed food additives "Nordex were PR is maintained in vivo test in pigs, weaned from the use of animal feed balanced nutritional standards in accordance with the recommendations of the GSI VNIIG RAAS and contaminated with mycotoxins in allowable amounts.
The results of the testing centre wildebeest Vivific for checking the contamination of feed ingredients that are used in JSC "Salinskoe" Sampurskogo district of the Tambov region is shown in table 4.
|The content of mycotoxins in feed ingredients, mg/kg|
|Barley without film||0,0||0,0||0,0||0,005||0,037|
The main components of the feed meets the requirements of GOST R 51530 "Feeds and concentrates for pigs, but had low toxicity.
Scientific and economic experiment was carried out on piglets from 2 to 4 months of age in pig-breeding complex of JSC "Salinskoe" Sampurskogo district of the Tambov region according to the scheme presented in table 5.
|The scheme of experiment on pigs, weaned|
|Group||The number of animals||terms of feeding|
|I - checksum||30||PC*|
|II - experimental||30||PC + Nordex (0.8 kg/ton of feed)|
|* PC (complete feed) in the control group as a means of prevention of mycotoxicoses when using weakly-toxic food contained known food additives Mycosorb and Biomass (Alltech, USA).|
Table 6 shows data on the composition and nutritional value of feed for two equal groups of 25 goals piglets paleochannel period.
|The composition and nutritional value of feed for piglets at weaning, %|
|Wheat grass alfalfa||2||2|
|Monochloride lysine 98%||0,40||0,40|
|Premix PANTO F-10||10||10|
|1 kg contains:|
|Metabolizable energy, MJ||12,68||Crude protein, g||172||172|
|Crude fiber, g||47||47|
|Methionine + cystine, g||7,9||7,9|
|Salt sodium chloride, g||3,5||3,5|
|Iodine mg||0,16td align="center"> 0,16|
|Vitamin f, time||16||16|
|Vitamin D, time||2||2|
|Vitamin U mg||115||115|
|Vitamin B12, mcg||30||30|
Conditions of pigs in the groups were identical.
The experimental feeding of livestock was carried out with dry feed twice a day according to the routine to the complex. Watering animals was plenty of sokovich supplies.
During the experiment conducted surveillance of the pigs and take into account the overall clinical the state, appetite, weight gain. At the end of the experiment at 120 days of age, animals were weighed and before feeding took the blood from the tail to determine the biochemical parameters. At the end of the experience, expertise on the microbial landscape of feces. Identified productive, zootechnical and economic efficiency of the sorbent Nordicos".
Basic zootechnical performances of broilers are presented in table 7, the basic zootechnical performances of piglets in table 8.
Feeding birds and farm animals experimental feed in combination with the studied additives showed that on the background of broiler chickens the first control group and during the piglets all the drugs gave a definite positive effect.
|Basic zootechnical performance of growing broilers|
|Indicators||group 1||group 2||group 3||group 4||group 5||group 6||7||8 group|
|Live weight, g:||Pin-role||PTA||Nordex 1 kg||Nordex 2 kg||PTA + Nordex 1 kg||PTA + Nordex 2 kg||Fungistatic||Mycosorb|
|in 20 days||770,86±11,13||771,1±16,01||802,7±12,65||799,3±10,52||795,5±12,34||787,8±11,96||802.3 requirements±10,56||808,8±are 11.62|
|in 36 days:|
|Average daily gain in live weight, g||59,3||61,0||60,8||60,4||61,8||61,6||61,1||61,5|
|1 head/day, g||repossessed a 93.44||94,11||94,20||94,34||94,01||94,11||94,06|
|1 kg gain, kg||1,63||1,60||1,60||1,62||of 1.57||1,59||1,60||1,58|
|The sex ratio|
|The productivity of piglets during cultivation|
|control (n=25)||experienced (n=25)|
|The live weight of piglets (kg):|
|when putting on experience||17,73±0,18||17,65±0,15|
|when removed from the experience||42,88±0,23||43,66±0,27*|
|The increase in live weight, kg||to 25.15±0,26||26,01±0,27*|
|± to the control group, kg||x||+0,86|
|Average daily gain in live weight, g||419±4||434±5*|
|In % of the control group||96,5||100,0|
|Spent feed per 1 kg increase in live weight, kg||4,17||the 3.65|
|In % of the control group||114,3||100,0|
|The safety of piglets %||100,00||100,00|
The use of the drug Nordex in the amount of 1 kg/ton of feed at the end of the cultivation was ensured by the increase of live weight of broiler chickens in comparison with the first group of 2.38-4,14%.
The use of the drug Nordicos at a dose of 2 kg/t of feed in feeds for broilers fourth, fifth and sixth groups contributed to the increase of live weight of chickens 1.75-of 3.77%. Moreover, a higher result in live weight broilers obtained from use of the drug Nordex dose of 1 kg/ton feed.
Fungistatic and Mycosorb improved live weight of broilers at 2,88 and 3,55%. Improvement in live weight of broiler chickens obtained at lower cost of feed per 1 kg increase in live weight.
The difference in the cost of feed on growth compared with the first control group was 0.6 to 3.7%.
The best result for the complex zootechnical performance obtained in the fifth the group receiving contaminated feed in combination with drug Nordex in the amount of 1 kg/ton of feed. The difference in live weight and cost of feed per gain in this group compared with the control amounted to 4.14% and 3.7% respectively, which also exceeded the figures for the other experimental groups.
When the slaughter was defined in absolute and relative weight of internal organs (table 9).
|The average absolute and relative weight of internal organs|
|Index||group 1||group 2||group 3||group 4||group 5||group 6||7||8 group|
|Live weight, g||2438,33||2540,00||2445,00||2318,33||2353,33||2435,00||2413,6 7||2386,00|
|Spleen, g||to 3.67||4,00||2,33||3,00||to 2.67||3,00||2,33||3,00|
Data analysis table 9 shows that the bird experimental groups there was a trend to increase in the relative weight of the liver and kidneys, with a slight decrease in the relative weight of the spleen.
Data on the content of vitamins in the liver are presented in table 10.
|The content of vitamins in the liver|
Data analysis table 10 shows that the content in the liver of broilers vitamins A, E and B2it was normal. However, all the studied additives, contributed to the decrease in the content of vitamin a in the liver of broilers experimental groups compared with control. The difference was 25,0-43,0%.
The use of feed additives Nordex, Fungistatic and Mycosorb not have an adverse effect on the deposition of vitamins E and B2.
The use of drugs Nordex in quantities of 1 and 2 kg/ton of feed contaminated with mycotoxins improved live weight of broilers at a lower cost feed on growth. Both drug efficacy was not inferior to the Fungistatic and Mycosorb. Best results are obtained when using the drug Nordex in the amount of 1 kg/ton of feed.
In respect of farm animals used for the use of the drug Nordex in feeds with low contamination by mycotoxins in the amount of 0.8 kg/ton of feed at the end of the growing improved live weight of piglets in comparison with the first group of 3.42%.
The feeding of the drug Nordicos at a dose of 0.8 kg/ton of feed for 2 months, had no negative influence on the organism of experimental animals.
In the first days of life pigs of all groups ate fodder not completely. In the future, the appetite of the young grow up, and feed intake was full.
In absolute terms, the average daily liveweight gain of piglets in the control group amounted to 419 g, and experienced - 435,
When feeding of the drug Nordex per 1 kg increase in live weight was spent 3,65 kg of feed compared to 4.17 kg feed with a sorbent of Mycosorb, that is the cost of feed for products decreased by 14.3%.
When feeding feed additives was not observed in digestive disorders and diseases of the gastrointestinal tract.
All experimental animals were in good physiological condition, there were no toxic effects and undesirable side reactions from feeding different drugs.
To assess the physiological status of animals and direction of metabolic processes in the body in piglets studied biochemical status of blood (table 11).
|Blood biochemical parameters of piglets (n=3)|
|Total protein, %||7,10±0,23||8,10±0,03*|
|Total calcium, mg%||11,27±0,37||11,33±0,59|
|Phosphorus, mg%||6,56±0,18||of 6.68±0,30|
It is established that in the experimental animals treated with "Nordex"intense held protein metabolism, contributing to the growth of the body mass, as indicated by a higher content in the serum total protein was 8.1%, compared to 7.1% (P<0,05).
The use of the drug Nordex in mixed fodders for pigs b is appopriate reflected not only on the protein, but on the mineral metabolism of calcium and organic phosphorus in the animal body, promoting health, which is consistent with the data of productivity and their physiological condition (table 11).
The blood of pigs in all treatment groups were within the physiological norm.
For the animals during the experimental period were daily clinical observation, however take into account the General condition, the average daily weight gain, diseases and timing recovery, the condition of skin.
Throughout the experimental period the animals treated with the sorbent Nordex, were in good physiological condition. Signs of toxic effects and undesirable side reactions from the use of sorbents was not observed.
At the end of the experience from the rectum piglets were selected feces samples for studies of microbial landscape (table 12).
|The faecal microflora of piglets at 120 days of age, CFU/g|
|Type of microorganisms||Group|
|control (n=3)||experienced (n=3)|
|Escherichia coli (E. Coli - typical)||104||104||104||104||104||104|
From table 12 it is seen that in the samples of faeces of piglets in both experimental groups was not detected enterococci and other opportunistic bacteria. The number of bifidobacteria and lactobacilli in piglets of the experimental group was significantly higher.
When the feeding of Guinea pigs sorbents Nordex and Mycosorb observed increased content of lacto - and bifidobacteria, which allows not only to restrain the propagation of pathogenic Escherichia and Salmonella (E. Coli - 104), but also to eliminate from the body of animals hemolytic forms of these bacteria, which contributes to a more sustainable intestinal microbiota the A.
In the study of faeces paid attention to their physical properties color, odor, consistency, and the presence of bubbles of gas, mucus, blood, pus, etc. Visual inspection did not give any specifics and deviations from the norm in all groups.
1. A method of obtaining a feed additive for the prevention of mycotoxicoses in animals and birds, consisting of mechanochemical treatment of the raw material components with subsequent microgranulate mixture, characterized in that as component a mixture of at least one mineral sorbent, hydrolytic lignin and cell wall of yeasts, while mechanochemical processing consists of crushing the individual components in the mills rotary-jet type with built-in classifier particles or a mixture of components and their treatment in the presence of at least one complex enzyme preparation, affecting residual polysaccharides hydrolysis of lignin and polysaccharides of the cell wall of yeast, after what shall microgranuloma the resulting milled mixture.
2. The method according to claim 1, characterized in that microgranuloma carried out in the dryer-granulator pseudocapsule layer, a rotary granulator, disc granulator or the granulator-mixer with high shear.
4. The method according to claim 1, characterized in that the grinding is carried out at a temperature of 40-85°C.
5. The method according to claim 1, characterized in that the grinding of the components is carried out until the particle size of 50-250 microns.
6. The method according to claim 1, characterized in that microgranuloma carried out at a temperature of the mixture components is not higher than 60°C to the size of the microgranules 200-800 μm and a residual moisture content of not more than 6%.
7. The method according to claim 6, characterized in that in the process of microgranulate spend additional chemical activation of hydrolytic lignin and cell wall of yeasts using at least one of the complex enzyme preparation, affecting residual polysaccharides hydrolysis of lignin and polysaccharides of the cell wall of yeast.
8. The method according to claim 7, characterized in that the complex enzyme preparation used dry enzyme preparation of Xiamen based on the fungal strain Trichoderma longibrachiatum.
9. The method according to claim 6, characterized in that in the process of microgranulate the composition of the mixture components introduce biologically active substances, which are distributed to the diet of animals and birds.
10. The method according to P9, characterized in that the biologically active substances are distributed to the diet of animals and birds, use lecithin in the amount of 10 to 40 g per 1 kg of the mixture components, Riboxin in the amount of 10-20 g per 1 kg of mixture components L-carnitine in the amount of 10-20 g per 1 kg of the mixture components, as well as podnikatel, inorganic or organic selenium compounds.
11. The method according to claim 1, characterized in that at the stage of microgranulate further added, at least one binder.
12. The method according to claim 11, characterized in that the binder agent used sucrose and/or 5%aqueous solution of carboxymethylcellulose.
13. The method according to claim 1, characterized in that as the use lignin lignin, rested in bed in the dumps 10-15 years with a moisture content of 5-25%.
14. The method according to claim 1, characterized in that as a mineral sorbent use shungite clinoptilolite, vermiculite, zeolite and/or montmorillonite.
15. The method according to claim 1, characterized in that the components of the raw materials taken in the following ratio: sorbent - 5-30 wt.%, lignin - 30-60 wt.%, the cell wall of yeasts - 5-50 wt.%.
FIELD: medicine, pharmaceutics.
SUBSTANCE: invention refers to medicine, namely pharmacology and may be used for cerebral protection in treating cerebral affections. That is ensured by using Zongorine as a cerebroprotective agent.
EFFECT: agent provides the cerebroprotective action and prevents the death in animals suffering experimental post-hypoxic encephalopathy due to the activation of cerebral neutral stem cells.
2 ex, 3 tbl
SUBSTANCE: invention refers to cosmetology and represents a cosmetic skin care product in the form of a water-in-oil emulsion, containing: (A) a volatile hydrocarbon oil 3 to 25 wt %, (B) a non-volatile silicon oil 2 to 15 wt %, (C) polyethylene glycol mono- or diisostearate 0.1 to 0.8 wt % containing 4 to 12 groups of oxyethylene, (D) polyoxyethylene and polydimethyl siloxyehtyl dimethicone copolymer 1 to 5 wt %, an d (E) organically modified clay mineral 1.5 to 3 wt %; the ingredients normally introduced into the skin care products - the rest.
EFFECT: provided excellent stability and no oily, slick and creaking sensations.
6 cl, 3 ex, 4 tbl
SUBSTANCE: invention refers to cosmetology and represents a cosmetic product for lips containing the ingredients (a)-(d): (a) hydrogenised polyisobutene 10 to 30 wt %; (b) one or more methylphenylsicicones 30 to 70 wt % to be separated when mixed with (a) at 25°C specified in trimethyl pentaphenyltrisiloxane, diphenyl siloxyphenyl trimethicone, diphenyl dimethicone and phenyl trimethicone, and containing trimethyl pentaphenyl trisiloxane; (c) one or more lipophilic substrates 0.5 to 8 wt % not separated when mixed both with the ingredient (a), and with the ingredient (b) at 90°C specified in sorbitane sesquiisostearate, propylene glycol monostearate, cetyl-PEG/PPG-10/1 dimethicone and diglycerol diisostearate; and (d) wax 5 to 12 wt %.
EFFECT: provided excellent effect of second adhesion stability immediately after application, long-lasting gloss and stability.
10 cl, 22 ex, 1 dwg, 13 tbl
SUBSTANCE: invention refers to chemical-pharmaceutical industry and represents a method of delivering a substance from an antiperspirant and/or deodorant composition to an individual's axillary space, involving the application of the anhydrous antiperspirant and/or deodorant composition onto the individual's axillary space, wherein: a) the anhydrous composition contains a base and a metal-organic frame structure formed of reusable porous material (RPM) and a substance adsorbed in the metal-organic frame structure and b) the reusable porous material has a structure degradable in contact with water and releasing a substance with the metal-organic frame structure formed of the porous material (RPM) specified in a groupu consisting of [Co3(biphenyldicarboxylate)34,4'-bipyridine], [Co(biphenyldicarboxylate)(4,4'-bipyridine)], [Zn2(biphenyldicarboxylate)2 (1,2-bipyridineethene)], Mg3(O2C-C10-H6-CO2)3 and combinations thereof.
EFFECT: provided longer release of a flavouring agent after the application of the deodorant/antiperspirant to the individual by the release of the flavouring agent at the moment of perspiration.
16 cl, 2 tbl
SUBSTANCE: invention refers to cosmetic industry and represents a non-foaming cosmetic composition of an oil-in-water emulsion containing (i) retinol, (ii) at least one polar emollient specified in a group consisting of propylene glycol stearyl ester, propylene glycol isostearate and mixtures thereof (iii) at least one non-polar emollient specified in a group consisting of aromatic or linear esters, Gerbe ester, mineral oils, squalane, isohexadecane, squalene, liquid paraffin and mixtures thereof with the weight ratio of the above polar emollient and the above non-polar emollient is found within the range of approximately 95 to 5 to approximately 40 to 60.
EFFECT: provided considerable reduction of retinoid-caused skin irritation and higher efficacy or retinoid.
11 cl, 3 ex, 7 tbl, 3 dwg
SUBSTANCE: surface lustre enhancing composition contains a pigment particle which is pre-coated with a cationic substance in amount of 0.1-10 wt % and isopropyl titanium triisostearate. The cationic substance is a substance selected from a group comprising quaternium, polyquaternium and a cationic polymer.
EFFECT: invention increases lustre of a surface on which a cleaning composition is deposited owing to retention, on the surface, of a pigment capable of increasing lustre.
SUBSTANCE: invention relates to obtaining biocompatible magnetic nano-particles and can be applied for therapeutic purposes, in particular, for fighting cancer. Method of obtaining nano-particles, including iron oxide and silicon-containing casing and having value of specific absorption rate (SAR) 10-40 W per g of Fe with field strength 4 kA/m and frequency of alternating magnetic field 100 kHz, contains the following stages: A1) preparation of composition of at least one iron-containing compound in at least one organic solvent; B1) heating of composition to temperature in range from 50°C to temperature 50°C lower than temperature of reaction of iron-containing compound according to stage C1 for minimal period 10 minutes; C1) heating composition to temperature between 200°C and 400°C; D1) purification of obtained particles; E1) suspending purified nano-particles in water or water acid solution; F1) addition of surface-active compound to water solution, obtained according to stage E1); G1) processing of water solution according to stage F1) by ultrasound; H1) purification of water dispersion of particles, obtained according to stage G1); I1) obtaining dispersion of particles according to stage H1) in mixture of solvent from water and water-mixable solvent; J1) addition of alkoxysilane into dispersion of particles in mixture of solvent according to stage I1); and K1) purification of particles.
EFFECT: invention makes it possible to obtain biocompatible magnetic particles with high value of specific absorption rate (SAR).
42 cl, 3 dwg, 9 ex
FIELD: medicine, pharmaceutics.
SUBSTANCE: invention refers to pharmaceutical industry, namely an agent for treating gastric mucosa erosions. The agent for treating gastric mucosa erosions representing a dry alcoholic extract of elevated parts of green violet.
EFFECT: preparing the agent for treating gastric mucosa erosions.
6 dwg, 2 tbl
FIELD: medicine, pharmaceutics.
SUBSTANCE: invention refers to pharmaceutical industry, particularly to a hepatoprotective herbal tea. The hepatoprotective herbal tea that contains black currant leaves, red raspberry leaves, great willow herb, meadowsweet herb in certain proportions.
EFFECT: herbal tea provides the effective hepatoprotective action.
FIELD: medicine, pharmaceutics.
SUBSTANCE: invention refers to pharmaceutics, namely using an aqueous-alcohol solution of Hymenocardia acida leaves for preparing a pharmaceutical composition for treating hypertension.
EFFECT: using the aqueous-alcohol solution of Hymenocardia acida leaves enables preparing the composition for treating hypertension which is well-tolerated by the patients if administered regularly and continuously.
10 cl, 4 dwg, 4 ex
FIELD: food industry.
SUBSTANCE: invention relates to confectionary industry. The tableted sweets production method involves ingredient selection, milling, sequential mixing and tableting. The ingredients are represented by sublimated vegetal raw materials - fruit, berries, vegetables, edible parts of plants, flower and plants extracts as well as dextrose, inuline and another prebiotic or probiotic additive. The vegetal raw materials are subjected to milling into particles sized 10-150 mesh; then one performs sequential mixing (in a "drum tumbler" type mixer) of, at first, dextrose with inuline and the other prebiotic or probiotic additive during 5-25 minutes, then one adds the sublimated vegetal raw materials and carries out the process during 10-30 minutes. Then one performs tableting in the press to produce 2.0-3.5 g sweets. The press hobs are preliminarily treated with a 1.8 % alcohol solution of stearic acid; alternatively, a lubricant, for example, stearic acid is introduced into the produced sweet mixture in an amount of 0.5-4%; the initial ingredients are taken at the following ratio, wt %: sublimated vegetal raw materials - 32-70, dextrose - 25-60, inuline - 5-20, another prebiotic or probiotic additive - balance. The probiotic additive is represented by lacto- and bifidobacteria, the other prebiotic additive is represented by "Prebiolact", fructooligosaccharides, galactooligosaccharides and pectin.
EFFECT: method ensures enhancement of taste and quality indices of the sweets, reduction of energy- and labour expenditures, simplifies the technological process along with preservation of useful properties of vegetal raw materials.
2 cl, 5 ex
FIELD: process engineering.
SUBSTANCE: invention may be used in food and pharmaceutical industries. Dish pelletiser with activator comprises inclined dish arranged to revolve in case with cover, dish drive, material loading and unloading branch pipes, nozzles, wall and bottom blades, driven activator arranged in cover perpendicular the dish bottom with clearance relative to dish bottom and wall. Activator drive is equipped with axial vibrator. Said activator is composed of a rod with frames arranged perpendicular to the flow of material being pelletised wherein radial plates are secured at two pints, that is, at the rod and at the frame.
EFFECT: faster pelletising, higher yield of 0,75-2,5 mm-fractions, uniform shape and density, reduced moisture content.
FIELD: food industry.
SUBSTANCE: dry probiotically active biopreparation contains Vitaflor" starter in the form of a porous tablet (with pores sized no less than 50% and activity no lower than 5x10 CFU/ tablet) and powder of sublimation-dried drinking milk with fat content of 1.5-2.5%. Tablet production involves dispensing in aluminium moulds, cooling to a temperature equal to minus 35-45°C in a 6-7 mm thick layer with subsequent maintenance at a temperature of minus 30-35°C. Sublimation drying is performed at the condenser-freezer temperature equal to no higher than minus 50°C under the working chamber pressure equal to 13-15 Pa during 48-50 hours. The crystallised moisture sublimation period lasts 20 hours at the material temperature equal to no higher than 30°C; additional drying is performed during 8 hours at a temperature of 25°C. One produces a tablet with 19.0±1.0 mm diameter and 6.5±0.5 mm height.
EFFECT: invention ensures a high probiotic potential of the biopreparation under extreme conditions, in particular, on board a space ship as well as under conditions of long-term self-sustained underwater navigation and the possibility of production of a high quality cultured beverage with probiotic properties and immunomodulatory effect.
3 cl, 4 dwg, 2 tbl, 8 ex
FIELD: food industry.
SUBSTANCE: invention relates to food industry, in particular, to physiologically functional ingredients production and may be used during functional food products manufacture using soya. Soya protein is produced by way of thermal acid coagulation of soya protein base with ascorbic acid. The vegetal oily base is introduced into the prepared vegetal raw material at the components ratio equal to 1:1.5 respectively. The vegetal base is represented by carrots or sea-buckthorn berries or rosehips or ash or haw or magnolia-vine berries. The vegetal oily base is represented by sunflower or soya or olive or sesame oil. The oily base and vegetal raw material are milled together to produce finest particles of the vegetal raw material. The mixture extraction is performed till production of a vitamin-and-fat base and insoluble vegetal residue. One mixes the vitamin-and-fat base, separated from insoluble vegetal residue, with soya protein at a ratio of 1:(2-9).
EFFECT: method allows to produce functional food products with balanced chemical composition and with high vegetal protein content.
4 cl, 4 ex
FIELD: food industry.
SUBSTANCE: invention relates to food industry. A water-dispersible granulate has average weighted weight diameter at least 100 mcm, preferably at least from 200 mcm and up to 2000 mcm. The said granulate contains: 40-90 wt % of carbohydrates; 2-30 wt % of lipids containing unsaturated fatty acids in an amount of at least 1%, preferably no less than 3% of the granulate weight and at least 30 mg/kg - 800 mg/kg of a metal enhancing oxidation and selected from the group consisting of ferrum, cuprum and their combinations wherein at least 50 wt %, preferably at least 70 wt % of lipids are present in the form of undispersed lipids.
EFFECT: invention allows to produce a granulate resistant to oxidation the production method whereof is implemented under mild conditions.
18 cl, 6 tbl, 3 ex
FIELD: process engineering.
SUBSTANCE: proposed invention may be used in production of semolina from animal feed by wet granulation technology. Proposed method comprises granulation of loose animal feed with addition of liquid component into press granulator, drying and cooling of granulated animal feed, crushing of obtained product and its fractionating into three fractions: large, medium and fine fractions. It includes also feeding large fraction for crusging and discharge of medium fraction as finished product. Said liquid component represents phototrophic biomass of micro seaweed added to animal feed in mixing in amount of 10…15%. Granulated animal feed is air dried at 70…80°C and with air flow rate of 2300…2700 m3/(h·t), and cooled by air at 7…10°C and with air low rate of 1200…1400 m3/(h·t). Medium fraction, prior to discharging it in the form of finished product, is dragged by vegetable oil fusa in amount of 10…12 wt % of medium fraction weight. Vegetable oil fusa is preheated to 60…70°C, enriched by anti-oxidising agents and fat-soluble ferments and filtered. Then obtained product is coated by fine fraction. Finished semolina is kept in ventilated hopper. Absorption refrigerator is used for recovery of cooling and hot air, as well as for heating water used for heating vegetable oil fusa. Proposed production line comprises animal feed hopper, press granulator, cooling column divided into drying and cooling zones, toll mill and two-sieve screening machine. Additionally, production line comprises site for seaweed growing, dragging site, vegetable oil fusa preparation site, finished semolina storage and absorption refrigerator.
EFFECT: expanded range of animal feed, increased biological value.
2 cl, 1 dwg
FIELD: food industry.
SUBSTANCE: treated food product production method envisages compressed air injection into a housing. The housing contains a section in the shape of frustum of cone. The injected air moves downwards through the housing and, having reached the lower end of the housing, flows back upwards and is released from the housing through an outlet hole. The treated food product with a water content of less then 14% (weight) and a lipid content of more then 15% (weight) is injected into the housing, captured by the injected air and milled. Then obtained granulated product is discharged from the lower end of the housing and mixed at least with one other ingredient of the treated food product. Then the treated food product is prepared from them.
EFFECT: invention allows to process the treated food product with a low moisture and a high fat lipids content, to implement a short-duration operation of granulation at one device without moving mechanical parts usage and to use the produced granulated product during treated food products production.
18 cl, 4 dwg, 2 ex, 1 tbl
FIELD: food industry.
SUBSTANCE: soya bean seeds are washed and wetted in water whereupon protein substances are crumbled, extracted and suspended in water. Further lacto serum is added into suspension as coagulant at ratio 1:1. Suspension is divided into solid and liquid fractions. Moisture contents of solid fraction is brought to 25-30%. Produced mass is granulated with dimension of granule diametre 1.0-2.0 mm. Further there is performed two-stage drying at temperature 110°C during 30 minutes and at temperature 180°C till appearance of specific golden-yellow colour. Also produced granules can be crumbled to a powder state.
EFFECT: invention facilitates production of natural food product containing vegetable and animal proteins, fats, adequate amount of carbohydrates and mineral substances with good organoleptic indices at relatively low cost due to implementing inexpensive components of its composition with long storage life increasing terms of selling and utilisation.
2 cl, 1 tbl, 2 ex
FIELD: food products.
SUBSTANCE: agglomerated food thickener contains pregelatinised starch and water-soluble covering agent. The agent contains substance chosen from the group including gum arabic, gum arabic treated with 1-oktenyl succinic anhydride, starches treated with 1-oktenyl succinic anhydride, maltodextrins treated with 1-oktenyl succinic anhydride, dextrins treated with 1-oktenyl succinic anhydride and mixtures of two or more of the above.
EFFECT: stable starch processed for its fast dispersibility both in hot and cold water.
20 cl, 2 dwg, 3 tbl, 4 ex
FIELD: food products, canned food production.
SUBSTANCE: invention relates to food-concentrates industry, namely to manufacturing of potato-based instant products. The dry mix contains potato product, protein component and food additives. Potato flakes are used as potato product, dry vegetable cream - as protein component, food additives are fat powder "Vekokrim", sodium glutamate, ribotides, tomato powder, salt, sugar, and additionally - ground black pepper, ground red pepper, dried bunching onion, dried parsley and broken onion with definite components proportion. The instant product contains the above mix for potato puree production and vegetable or meat-and-vegetable canned food with definite components proportion. The invention allows reaching acceptable consumer consistency of end product, well-balanced composition and optimal organoleptic properties.
EFFECT: manufacturing of a product with increased digestibility and optimal organoleptic properties.
4 cl, 2 ex
FIELD: food industry.
SUBSTANCE: method envisages heating the meat part to a temperature higher than the denaturation point till a meat composition formation. Additionally one combines one or more carbohydrates sources and water with a carbohydrate composition formation. The carbohydrate composition is heated to the gelatinisation temperature. The meat composition and the pre-hydrated and pre-gelatinised carbohydrate part are combined.
EFFECT: invention ensures preservation of natural and perceptible characteristic features of skeletal muscles and/or meat by-products as well excludes undesirable impact of meat components to the carbohydrate composition treatment process.
19 cl, 2 dwg