Preparation for cleaning of soil from oil and oil products
SUBSTANCE: preparation containing biodestructor of oil contamination represents centrate of cultural liquid of microbial mass of consortium of oil-oxidising microorganisms immobilised on peat carrier. Consortium composition includes the following: Rhodococcus eqvi SRI MCC B-1115 bacteria strain, Rhodotorula glutinis SRI MCC Y-1113 yeast and Rhodotorula glutinis ARSRI MCC Y-1114 yeast strain in the ratio of 1:1:1 respectively, which have been grown jointly.
EFFECT: invention allows improving quality of soil cleaning from oil and oil products under conditions with reduced temperatures.
The invention relates to the oil industry and ecology and can be used for cleaning oil-contaminated soil.
One of the most dangerous pollutants are oil and oil products, annual penetration of which into the environment is estimated at hundreds of millions of tons. In practice reclamation works when cleaning oil-contaminated soil using biological oxidizing action is not the last place.
Known drug-biodestruction oil pollution microbial-enzymatic Microsim(TT) "PETRO THREAT" (http://www.microzym.ru/oilspills.htm), producer LLC RSA trading, sanitary-epidemiological conclusion No. D from 11.03.2005. The drug represents a completely natural biological destructor petroleum hydrocarbons intended for environmentally safe cleaning soil and water bodies from oil pollution. As the active elements of the drug contains the consortium of (12) strains of living of hydrocarbon-oxidizing microorganisms in the form of concentrate dry spores concentration equal to 4×1012CFU/g, the set of natural microbial enzymes, mineral salts of nitrogen, potassium, phosphorus, organic feeding media from food corn flour, organic fertilizer.
The drug is intended for the biological is tion clean soil covers, sand, sludge, wastewater, water bodies and other non-aggressive environment from pollution by oil and oil products: gasoline, diesel fuel, fuel oil, motor oils, crude oil.
Disadvantages PETRO TRITA are:
- increasing production costs, as in the preparation contains more strains of microorganisms;
carrier PERTO TRITA food is corn flour - less accessible in remote Northern regions and therefore more expensive to import;
- the drug is not able to carry out the cleanup of oil and oil products in environmentally adverse conditions.
The basis for the production of biological products are strains of crops oil-oxidizing microorganisms. In most cases, the target product of the process of cultivation in the production technology of the bacterial preparation is microbial mass, and the remainder after division, spent culture liquid (centrate)is a waste of production. After centrifugation, the centrate is collected in special containers where subjected to heat treatment, after which it merges. Draining of supernatant entails environmental pollution and costs associated with environmental events. As centrate, enriched with biologically active substances, can be used for bi the remediation of contaminated oil and petroleum products land.
The task of the invention to provide a new drug capable of disposal of oil and oil products from the soil, when environmental conditions are unfavorable to the activity of microorganisms. Under no conditions implied low temperature, high humidity, Cromartie soil etc.
The technical result is to improve the quality of cleaning soils from oil and oil products in Arctic conditions, the possibilities for use in regions with low temperature environment, in contrast to microbial agents.
The technical result is achieved by the fact that the drug for cleaning soil from oil and oil products containing biodestruction oil pollution, according to the invention represents centrate cultural liquid microbial mass consortium of oil-oxidizing microorganisms immobilized on thoroseal, and the consortium is composed of: a bacterial strain Rhodococcus eqvi B-l 115, a strain of the yeast Rhodotorula glutinis Sri ECR Y-1114, a strain of yeast research Institute ECR Rhodotorula glutinis Y-1113, taken in the ratio 1:1:1, grown together.
Centrate, which is in the industrial production of microbial agents is a waste, is a culture fluid containing metabolic products of bacteria, including extracellular enzymes, vydeleny the environment in the process of growing microbial mass, which are resistant to adverse conditions compared to biomass without centrate. Immobilization centrate on thoroseal (adsorption mixing and drying of the supernatant with peat) creates additional resistance to inhibitors and is a condition for storage and transport of extracellular enzyme complex.
Due to resistance to adverse environmental factors (under unfavorable environmental factors include low temperature, high humidity, Cromartie soil etc.) extracellular enzymatic activity centrate allows the degradation of petroleum hydrocarbons in the soil, while for microbial mass, which is the basis of biological data on environmental factors is negative.
Strains of microorganisms obtained by extraction from oil-contaminated soil in a nutrient medium of čapek, of the following composition: sucrose 30 g, agar 20 g, NaNO3- 5,0 g, MgSO4- 0.5 g KCl, 0.5 g, KH2PO4to 3.0,
Strains of the yeast Rhodotorula glutinis 55-1-P, Rhodotorula glutinis u-CB # 26 and bacterial strain Rhodococcus eqvi 34-1(28-99/2) deposited in the research culture Collection of microorganisms" fsri SRC VB "Vector". Registration number: Y-1113, Y-1114, fill 15, respectively.
Source selection strain of the yeast Rhodotorula glutinis 55-1-P: oil-contaminated soil Usinsk district republics of the Komi vozeiskoe oil fields, 1999. Colony bright pink, convex, smooth, shiny slimy, smooth edge. Cells oval, 3-7 X4 - 15 μm, polar budding.
Source selection strain Rhodotorula glutinis u-CB # 26: oil-contaminated soil Usinsk district of the Komi Republic SLE. 203 Verkhnevizeiskogo oil field, 1995. Colony bright pink, convex, smooth, shiny slimy, smooth edge. Cells awalnya, 3-7 X 4-15 μm, polar budding.
The source selection of the bacterial strain Rhodococcus eqvi 34-1(28-99/2): oil-contaminated soil Usinsk and vozeiskoe deposits of the Republic of Komi, soil, fresh and old oil spill. Colonies on nutrient agar round, convex, up to 5 mm in diameter, smooth, shiny, cream-colored, with age - pale pink; cell life cycle - sticks-cocci.
The method of obtaining the supernatant includes:
- joint to improve the main cultures of microorganisms: Rhodotorula glutinis 55-1-P, Rhodotorula glutinis u-CB # 26, Rhodococcus eqvi 34-1(28-99/2), isolated from oil-contaminated soil in the flasks 100 ml Chapek's medium with addition of oil, within 3 days, in the ratio of 1:1:1;
- obtaining the supernatant by dividing jointly developed cultures of microorganisms: Rhodotorula glutinis 55-1-P, Rhodotorula glutinis u-CB # 26, Rhodococcus eqvi 34-1(28-99/2), by centrifuging for microbial mass and the supernatant 2,7 thousand revolutions per minute for 8-10 minutes (narab is determined jointly microorganism cultures spread across the tubes and separated in a centrifuge to sediment-microbial mass and the culture fluid is centrate);
- immobilization centrate on thoroseal (adsorption mixing of effluents and peat at a rate of 1 cm2× 100 cm2(15 g per 1 kg) with subsequent drying).
In the laboratory of the Institute was tested method of manufacturing the supernatant, comprising the following steps:
Step 1. Joint to improve the main cultures of microorganisms: Rhodotorula glutinis 65-1-P, Rhodotorula glutinis u-CB # 26, Rhodococcus eqvi 34-1(28-99/2), isolated from oil-contaminated soil in the flasks 100 ml Chapek's medium with addition of oil for 3 days:
culture of microorganisms have been made in liquid medium of čapek bacteriological loop in the ratio of 1:1:1 to 10 μl at room temperature under sterile conditions;
oil added to the flask as an additional power source in the amount of 0.5 ml;
- zarabatyvanie biomass of microorganism cultures were performed in temperature-controlled setting the microorganism growing WVMT-12-250 at a temperature of 30°C and 180 rpm for 3 days.
Step 2. Obtaining the supernatant by centrifugation of the culture fluid with microbial mass:
- jointly developed within 3 days of the microbial biomass was poured into centrifuge tubes were placed in a centrifuge (the COUNTRY CLMN-P10-02 and perform the separation of the biomass at 2.7 thousand rpm for 8-10 minutes on centrate and microbial mass;
the separation of supernatant (supernatant in the centrifuge tube) from microbial biomass (residue in the centrifuge tube) was performed in a sterile box by draining the supernatant into a sterile tube. At the bottom of the centrifuge tube remained settled cells microbial mass.
Step 3. Immobilization of supernatant adsorption binding thoroseal:
- Centrate was infused into peat, with constant stirring at a rate of 1 cm2× 100 cm2(15 g of supernatant for 1 kg of peat). Characteristics of peat are presented in table 1:
|Agrochemical characteristics of peat|
|pH||P2About5(mg/100 g||K2O, mg/100 g||CA, mg/mEq 100 g||Mg, mg/mEq 100 g||N%||S with%|
- Drying of peat took place at room temperature T=21-23°C to air-dry state.
In the laboratory trials were conducted made of supernatant, immobilized on thoroseal for the optimization process, the money is Adachi oil contaminated soil substrates. Immobilized on peat centrate was made in the soil substrates, we determined the degree of degradation of petroleum hydrocarbons after certain intervals of time. Used centrate composition of microorganisms Rhodotorula glutinis, Rhodococcus erythropolis, Rhodococcus eqvi, taken in the ratio 1:1:1, jointly developed. For comparison felt appropriate microbial mass. As objects of study used sand, clay and peat substrates, the oil in concentrations of 50 and 150 mg/year Experienced substrates were contaminated with oil.
On contaminated substrates (table 2) were made centrate immobilized on the peat at a rate of 1 cm × 100 cm2(15 g/kg), and (for comparison) microbial mass with a titer of cells in the working solution of 1.2·109(the solution was brought to 5 ml/kg of air-dry soil). Additionally made of mineral fertilizer - ASFC (N16P16K16) (350 kg/ha (35 g/1000 cm2in aqueous solution, NMat the rate of 100 kg/ha (10 g/1000 cm2). Once samples were taken for tests: enzyme activity (catalase, dehydrogenase, lipase), the intensity of CO2residual oil content in the soil. Further analysis samples were taken 3 days after the start of the experiment, 15 days, 30 days and 6 months. The research results presented in table 1, taken from the source and destination parameters.
The action of lipases aimed at splitting of ester bonds between glycerol and high molecular weight fatty acids with formation of free fatty acids, di - and monoglycerides and glycerol. Change lipase activity is associated with the accumulation of low-and non-degradable biological substances formed during biodegradation of crude oil, but also from the fact that in the degradation of lipids participate enzyme system, very similar to the systems of oil biodegradation.
In self-cleaning oil-contaminated substrates leads to a minor change in the enzymatic activity by the end of the experiment. Basically there is a decrease in catalase, dehydrogenase activity and lipase activity. The introduction of oil-contaminated substrates as centrate with mineral fertilizers and microbial mass increases biochemical processes. In clayey and sandy substrates increased respiratory activity in the variants with the introduction of supernatant.
Table 1 shows the experimental results and comparative analysis. Adopted the following conventions - Control - soil, uncontaminated oil and neopentanoate and microbial mass, 1 - oily soil without treatment of the centrate and microbial mass, 2 - contaminated soil + centrate on thoroseal and mineral fertilizer, 3 - contaminated soil + microbial mass and mineral fertilizer, I - raw data (enzymatic activity, the intensity of selection WITH2in the beginning of the experiment), II - final data (enzymatic activity, the intensity of selection WITH2after 6 months of production experience).
As can be seen from table 1, by the end of the experiment the substrates is a noticeable reduction in the level of oil pollution (10-40%) as with the introduction of supernatant, and with the introduction of microbial mass.
Sandy substrate unlike clay and peat is characterized by low biological activity, low content of biogenic elements, i.e. the most environmentally unfavorable for purification from oil products. The introduction of oil increases lipolytic processes and dehydrogenation processes, but the degree of purification in the polluted substrate 6 months low. Introduction microbial mass or centrate improves biochemical activity. When making centrate amplifies the intensity of the allocation of CO2. The greatest degree of cleaning in sandy substrate in the variant with the introduction of supernatant.
Thus, due to resistance to adverse environmental the practical factors of biodestruction oil pollution can enhance the degradation of oil in soil at high oil load and at low ambient temperature, even in situations where the activity of microbial destruction of oil is minimized by these factors. The drug allows you to change the composition and structure of oil pollution and make it more accessible to microbial attack. Competitive advantages of the product is the availability and cheapness in manufacture, based on the use of waste products and is available for Northern conditions thoroseal.
Preparation for cleaning soil from oil and oil products containing biodestruction oil pollution, representing centrate cultural liquid microbial mass consortium of oil-oxidizing microorganisms immobilized on thoroseal, and the consortium is composed of: a bacterial strain Rhodococcus eqvi Institute of PFC In-1115, the strain of yeast Rhodotorula glutinis Sri ECR Y-1114 and the strain of yeast Rhodotorula glutinis Sri ECR Y-1113, taken in the ratio 1:1:1, respectively, grown together.
SUBSTANCE: Bacillus licheniformis strain has antagonism in relation to Salmonella typhi, Staphylococcus aureus, Listeria monocytogenes and resistance to the following antibiotics: streptomycin and nalidixic acid. The strain is deposited with number BKM B-2712D in the All- Russian collection of microorganisms of the Institute of Biochemistry and Physiology of Microorganisms of the Russian Academy of Science (IBFM RAN).
EFFECT: strain can be used for creation of probiotic bacterial preparations in veterinary.
SUBSTANCE: strain is deposited in CNCM and has number 1-3689. Strain has ability to inhibit the growth of pathogenic microorganisms in culture, and namely Escherichia coli, Salmonella enteritidis and Listeria monocytogenes. Strain has anti-inflammatory properties at the ratio of IL-10/IL-12 equal to 11.8.
EFFECT: food product containing the above living strain has antimicrobial or immunomodulating properties.
6 cl, 3 dwg, 4 tbl, 3 ex
SUBSTANCE: Bacillus licheniformis VKM B-2713D strain has apparent antagonism in relation to Salmonella typhi, Staphylococcus aureus, Listeria monocytogenes. Strain has resistance to antibiotics at streptomycin concentration of 20 mcg/ml and at concentration of nalidixic acid of 20 mcg/ml. Strain has α-amylase activity of 0.015 U/ml of the medium at pH 6.0, protease activity of 2.34 U/ml of the medium at pH 7.0.
EFFECT: improving activity.
1 tbl, 3 ex
SUBSTANCE: invention proposes a method for obtaining recombinant core protein of hepatitis E virus (rtHEV-ORF2) and recombinant vaccine for prophylaxis of hepatitis E virus. Core protein is obtained by cultivation of recombinant yeast strain Hansenula polymorpha "КБТ"-11/pHEV-001, which contains DNA sequence integrated into genom of yeast cell and coding the fragment of amino-acid sequence from position 86 to 607 of core protein of hepatitis E virus of genotype 3 (rtHEV-ORF2) under control of promoter of MOX gene. The method allows obtaining immunogenic antigene of hepatitis E virus, which has properties of natural protein. Based on the obtained antigene there created is recombinant vaccine for prophylaxis of hepatitis E virus. Vaccine includes effective amount of rtHEV-ORF2 protein, adjuvant and a physically acceptable diluter.
EFFECT: vaccine is immunodominant and non-toxic and has no by-effects.
3 cl, 5 ex
SUBSTANCE: invention represents Bacillus licheniformis bacteria strain All-Russian collection of industrial microorganisms B-11302 - producer of heat-resistant lipase. At fermentation of the obtained strain in 3 l of a laboratory fermenter the ferment activity in culture liquid reaches the level of 500 U/ml.
EFFECT: invention allows obtaining heat-resistant lipase with high efficiency degree.
3 dwg, 5 ex
SUBSTANCE: at fermentation of obtained strain in 3 l of a laboratory fermenter in minimal medium at the temperature of 30°C, ferment activity in culture liquid reaches the level of 210 U/ml.
EFFECT: invention allows obtaining Specific Phospholipase with high efficiency degree.
3 dwg, 5 ex
SUBSTANCE: production method of keratinase provides for directed adaptation of strain Penicillium citrinum PC-54-91"ВИЛАР" by three-time subcultivation on Czapek agar medium containing 2% of hair keratin as a carbon source. Cultivation under deep conditions on Czapek medium containing 10% of hair keratin and 0.5% of saccharose during 6 days. Separation of biomass from culture fluid with further extraction of target product by two-stage chromatographic cleaning involving gel filtration on TSK-Gel TOYOPEARL HW-40 and affine chromatography on protein A to sepharose CL-4B with further lyophilisation.
EFFECT: invention allows increasing ferment yield, obtaining keratinase preparation decomposing the hair α-keratin, and improving cleaning degree of ferment preparation.
2 tbl, 5 ex
SUBSTANCE: liquid culture of strain Corynebacterium glutamicum All-Russian collection of industrial microorganisms B-1959 - lysine producer by means of a deep method and liquid cultures of strains Bacillus subtilis All-Russian collection of industrial microorganisms B-8130, Bacillus subtilis All-Russian collection of industrial microorganisms B-2984, Bacillus subtilis All-Russian collection of industrial microorganisms B-4099 and Bacillus licheniformis All-Russian collection of industrial microorganisms B-4162 are prepared. To liquid culture of strain Corynebacterium glutamicum All-Russian collection of industrial microorganisms B-1959 there added in quantity of 30 l is 35 l of the mixture consisting of liquid cultures Bacillus subtilis All-Russian collection of industrial microorganisms B-8130, Bacillus subtilis All-Russian collection of industrial microorganisms B-2984 and Bacillus subtilis All-Russian collection of industrial microorganisms B-4099, which have been taken in the ratio of 6:6:1 respectively. Common mixture of liquid cultures is applied onto the carrier prepared in advance - sterile exhausted beet pulp treated with cellulolytic ferment and enriched with fermentolysate of Saccharomyces cerevisiae yeast. Then, it is mixed and exposed; after that, solid-phase fermentation is carried out under the specified conditions of restricted oxygen access. Liquid culture Bacillus licheniformis All-Russian collection of industrial microorganisms B-4162 containing at least 5.6×108 CFU/g is added in the amount of 65 l. The mixture is mixed and dried to the humidity of 8-10%. Dry powders of purple echinacea and fruits of holy thistle are added in terms of 20-50 g per 1 kg of the end product. The obtained mixture is stirred and subject to crushing till indiscrete mass is obtained.
EFFECT: invention allows improving feedstuff quality.
3 tbl, 1 ex
SUBSTANCE: strain Aspergillus oryzae RCAM01135 is a producer of proteolytic and amylolytic ferments, which has ability to produce proteolytic and amylolytic ferments. It was deposited at GNU VNIISKHM with the following registration number: RCAM01135. It can be used at production of different food products (fermented spices, additives, and drinks).
EFFECT: invention allows increasing the growth speed and intensity of spore formation.
4 tbl, 2 ex
SUBSTANCE: strain of unicellular algae Dunaliella salina IPPAS D-295-producer of biologically active substances having antioxidant activity was deposited in the culture collection of microalgae of the Institute of Plant Physiology n.a. K.A.Timiryazev RAS (IPP RAS (IPPAS)) under registration number IPPAS D-295 and can be used to produce biologically active substances having antioxidant activity and antagonistic activity against opportunistic pathogenic bacteria.
EFFECT: invention enables to increases the yield of antioxidant substances.
3 tbl, 2 ex
SUBSTANCE: proposed chimeric protein with SEQ ID NO:02 is fluorescent biosensor, built on the basis of HyPer protein and mutant of PH-domain of Btk tyrosine kinase.
EFFECT: proposed inventions allow performing simultaneous monitoring of product of hydrogen peroxide and phosphatidyl inositol-3,4,5-triphosphate in a living cell.
4 cl, 4 dwg, 3 ex
SUBSTANCE: 1% sterile solution of glucose is prepared on the basis of a physiological solution, which is used as a nutrient medium. An absorber by Zaytsev is connected to an aspirator of "Briz-1" grade, into the flask of which 10 ml of the prepared 1% glucose solution is placed. The device is placed into the investigated room, and the aspirator is switched on for 15 min. Microorganisms that are in air go through the glucose solution and are retained in it. The solution is placed into a test tube and thermostatted at 37°C for 2 hours. Solution electroconductivity is measured with the help of a sensor KDS-1038. The number of microorganisms in air is determined according to the curve of empirical dependence of solution electroconductivity on the number of microbes, which is built in accordance with the produced values.
EFFECT: invention makes it possible to reduce time for determination of microorganism number in the air of the working area down to 2 hours and 20 min.
SUBSTANCE: method to protect yeast Saccharomyces cerevisiae against oxidising stress as a result of exposure to hydrogen peroxide includes growing of yeast culture under standard conditions till the end of the logarithmic or the start of the stationary phase of growth, incubation with a protective agent, exposure to hydrogen peroxide with subsequent determination of the number of survived cells. The protective agent is represented by soy inhibitors of proteases in concentration of 0.1-2.0 g/l. The time of incubation with the protective agent makes 5-6 hr, concentration of hydrogen peroxide is 700-750 mM. The invention provides for high extent of yeast cells production with no depressant action.
EFFECT: share of survived yeast cells during method realisation increases in comparison with the reference one 1,2-5,4 times.
SUBSTANCE: composition for production of organosilicon sol-gel matrix for immobilisation of microorganisms in biosensor analysers is provided. The composition consists of 20% polyethyleneglycol solution in phosphate buffer solution, Tetraethoxysilane, and 0.2 mol/dm3 catalyst solution NaF, an additionally introduced hydrophobic additive - methyltriethoxysilane. The components are taken at a volume ratio of PEG:TES:MTES: NaF 4:(18-3.4) (2-16.6):1.
EFFECT: reducing the toxic effect of the matrix on the biomaterial and increase in its mechanical strength.
1 tbl, 1 ex
SUBSTANCE: present invention relates to microbiology and a method of detecting and counting viable Legionella pneumophila microorganisms in a sample. The described method involves: (1) contacting said microorganisms of said sample with at least one reducing compound which contains glutamate and pyruvate, and a culture medium which is a buffered charcoal yeast (BCYE) or GVPC agar culture medium, (2) incubating the product of step (1), and (3) detecting and determining the number of viable microorganisms. The reducing compound used directly or indirectly affects metabolism, reducing oxidative stress of the microorganism by reducing formation and/or breaking down reactive forms of oxygen.
EFFECT: disclosed method enables to accurately count Legionella pneumophila microorganisms in a stressed condition.
7 cl, 5 dwg, 5 ex
SUBSTANCE: method of qualitative assessment of biocorrosion damages of thin-walled sealed enclosures of aluminium-magnesium alloys in operation of spacecrafts and the suspension of spore materials fungi of implementation of the said method is proposed. The test and control samples of aluminium and magnesium alloys are prepared. The prepared samples are dried, sterilised. Fungal cultures - strains of microorganisms Paecilomyces variotii Bainier of All-Russian collection of microorganisms F-4039D, Ulocladium botrytis Preuss of All-Russian collection of microorganisms F-4032D, Penicillium chrysogenum Thorn of All-Russian collection of microorganisms F-4034D, Aspergillus sydowii (Bainier et Sartory) Thorn et Church of All-Russian collection of microorganisms F-4037D, Cladosporium sphaerospermum Penz. of All-Russian collection of microorganisms F-4041D are inoculated for growing spores into tubes with a sloped agar medium of Czapek-Dox. The tubes are thermostated at a temperature of (29+2)°C for 14-28 days until appearance of mature spores. Spore materials suspensions of individual cultures of the said fungi are prepared, which are then mixed in equal proportions. And the concentration of spores of each fungi species in the suspension should be in the range of 1-2 mln/cm3. The resulting suspension is applied to the sterilised test samples. Then they are dried and placed as one sample in each Petri dish on the surface of agar medium of Czapek-Dox. The control samples not treated by the spore material are also placed on the surface of the agar medium of Czapek-Dox in the Petri dishes. The Petri dishes with the test and control samples are placed in different desiccators at the bottom of which water is poured to maintain the humidity more than 90%. The desiccators are closed and incubated in the thermostat at a temperature of (29±2)°C. The exposition of test and control samples is carried out for 40, 120 and 180 days. Then the samples are removed from the Petri dishes, and the corrosion products and mycelium are removed from the surface of the samples washing them in running water. The samples are soaked in 70% ethyl alcohol for 30 minutes, then they are washed with detergent and dried. Then, using a scanning electron microscope a qualitative assessment of biocorrosion damages is carried out. According to the change in the appearance of the surface of the samples the initial stages of biocorrosion and its type is evaluated, as well as the distribution of corrosion damage on the surface of the samples, and the dependence of the biocorrosion process on time is determined.
EFFECT: inventions enable to carry out the qualitative assessment of the initial stages of biocorrosion damages of thin-walled sealed enclosures of aluminium-magnesium alloys with the thickness of more than 2 mm.
2 cl, 9 tbl
SUBSTANCE: invention relates to means of controlling quality of organic and inorganic products, and can be used to evaluate safety of food and fodder products, natural water and waste water, ground, soil, determining the maximum allowable concentration of contaminants, as well as the impact of human activities on the environment, including oil extraction and refining products. The apparatus consists of a computer with a software system and a biodetector. The biodetector has a housing 1 inside of which there is a displacement controller for a board 2 with containers 3 for test objects, a light source - light-emitting diodes 4, an optical system with a television camera 5 and a lens 6 mounted on a support 7. The apparatus is provided with a light-proof casing 8 for closing the top of the board with containers for test objects, the inner surface of which is coated with white paint. The optical system with a television camera 5 and a lens 6 is mounted on the support 7 by a turning mechanism 9 in form of a ball head. Inside the housing 1 there is a stepper motor 10 for rotating the board 2 with containers 3.
EFFECT: invention increases measurement accuracy while reducing the measuring time, simplifying the design and reducing the size of the apparatus.
2 cl, 2 dwg
SUBSTANCE: device comprises a through cell equipped with holes, where at least one hole represents an inlet hole for intake of fluid medium from the specified technological flow, and at least one hole is an outlet hole for discharge of fluid medium from the specified through cell. To one of specified holes an RK probe is attached, possibly, an OVP probe, a cleaning accessory. The first pipeline is connected to the inlet hole. Possibly, the second pipeline is connected to the outlet hole. A valve is connected to the specified through cell. With the help of the specified devices and methods they measure volume microbiological activity and surface microbiological activity in a process flow of water by means of measurement of dissolved oxygen concentration.
EFFECT: method improvement.
45 cl, 10 dwg, 2 tbl, 4 ex
SUBSTANCE: method includes the following stages: contact of a sample with a source of nutrition for cells, containing antioxidant, representing pyroracemic acid or its salt, and an inhibitor of cell proliferation, which is selected from ciprofloxacin and cefalexin; contact of the specified sample with fluorescent-marked oligonucleotide probes, capable of specific hybridisation at least with one section of ribosomal nucleic acids, which belong to microorganisms of Legionella pneumophila kind and type; and detection and quantitative determination of a fluorescent signal.
EFFECT: provided method and set make it possible to more accurately and reliably detect and calculate viable microorganisms of Legionella pneumophila type, having excluded natural fluorescence of microorganisms from calculation.
6 cl, 2 dwg, 6 tbl, 2 ex
SUBSTANCE: present invention relates to chemical industry and agriculture and is a plant growth stimulating agent.
EFFECT: invention enables to produce an agent capable of regulating plant growth or differentiation.
16 cl, 22 ex, 11 dwg
SUBSTANCE: invention relates to engineering ecology. The apparatus for finish treatment of coastal sea water, which is a sanitary algae plantation, having propylene power cables with diameter of 30-40 mm, held in a horizontal position by wire cables attached to gravity anchors through fastening elements of buoys connected to the power cables. The apparatus is provided with working modules held on the surface for holding fucus algae measuring 2×1.5 m, made from synthetic ropes with diameter of 10-20 mm, which are substrates for the fucus algae. The modules are attached to the propylene power cables or wire cables by floats to enable fastening of the modules in the required position relative the direction of movement of contaminated water depending on weather conditions. The apparatus has vertical ropes attached to the power cables, which are a substrate for drift weed, with length of 5-12 m and equipped with loads which provide their tension.
EFFECT: invention increases the efficiency of finish and preventive treatment of coastal sea water from oil products, toxic metal and household wastes.
2 cl, 2 dwg