Biologically active biomaterial production method and biomaterial produced by said method. RU patent 2500198.

FIELD: food industry.

SUBSTANCE: invention relates to food industry, in particular, to biomaterial production from by-products of production of an alcohol-free beverage based on tea fungus. The method for production of a biologically active biomaterial based on a natural composition is as follows: the composition is represented by tea fungus zooglea produced by way of incubation under aerobic conditions in a nutritional medium at room temperature during 14-160 days. The nutritional medium is represented by a sugar-containing product solution in tea infusion. Then zooglea is separated from the nutritional medium and dried at a temperature no more than 55°C till the weight fraction of the dry substances is equal to more than 70 wt %.

EFFECT: invention allows to manufacture a biologically active material with improved preventive properties and "human - new biomaterial" compatibility properties as well as ensures environment improvement along with environmental friendliness enhancement during the new biomaterial disposal, the proposed biomaterial biomass growth acceleration and the biomass growth uniformity enhancement.

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The technical field

The invention relates to a method of obtaining biologically active biomaterials, including in industrial conditions, and to biomaterials, obtained by the proposed method, and is a by-product during the production of soft drink Kombucha-based.

Prior art

Currently very important in the production of products manufactured from natural raw materials rich in valuable biologically active components.

Constant aspiration of people to maintain their health and the problem of waste makes the actual task of creation of similar preparations.

There are a large number of different biomaterials used in the medical industry, for example, as implants for different purposes, medical , mainly obtained by synthesis of organic compounds and trace elements (see, for example, [Application 2007112777/15, EN from 03.10.2005, patent 2321597, EN from 17.04.2003], www.smolzdrav.ru).

Known artificial Rostoshi" (see, [Application 2005102040 from 28.01.2005])representing for the treatment of burn patients and contains the components of the human skin, which included active peptides placental origin of mammals and native molecule hyaluronic acid. Release «», which is dissolved in the wound as healing, does not require bandages and removal of material remains (see, http://hyamatrix.ru/products/bioplastic/). Known, for example, a new type of dressing materials based on starch, developed by researchers under the guidance of Kong (see, http://www.mhealth.ru/health/healthnews/bioplastyr-nakleil-i-zabyl/#ixzz210SgQ6Ne). Once on the skin, such dressings eventually decomposes, turning into glucose, which is absorbed by the body. Nanofibres, of which taken , were extracted from a liquid starch environment by means of the electric field, and then woven into similar to the sample paper for the manufacture of , dressing, toilet paper, napkins and other products.

ways of getting people's home-based beverage cultural liquid Kombucha, possessing biological activity (see, for example, [LT Danielyan. Kombucha (kombucha) and its biological features. Moscow, «Medicine», 2005, cc.32...40], [I.P. . Kombucha. Natural healer. Moscow - St. Petersburg, «Dilya», 2007, cc.89, 90], [A. Semenova. Healing tea vinegar. St. Petersburg, Nevsky Prospekt, 2002, cc.47, 48]).

Known for highly active biological concentrate cultural liquid Kombucha (see [LT Danielyan. Kombucha (kombucha) and its biological features. Moscow, «Medicine», 2005, cc.39...46, pp.163 paragraph 3 lines 1-3].

The use of directly Kombucha for the manufacture of any of biomaterials and we currently unknown.

Disclosure of the invention

The technical result of the invention - biologically active biomaterial is the expansion of the class of organic biologically active biomaterials for increased range of products with improved preventive properties and properties compatibility «man - new biomaterial», improving the environment while improving environmental friendliness during utilization of the new biomaterial, the acceleration of growth of biomass proposed biomaterial and increased uniformity of its growth and development of technology of industrial production of a new biomaterial.

The technical result of the invention - a method of obtaining biologically active biomaterial is the expansion of the class of organic biologically active biomaterials for increased range of products with improved prevention, properties and properties compatibility «man - new biomaterial», improving the environment while improving environmental friendliness during utilization of the new biomaterial, the acceleration of growth of biomass proposed biomaterial and increased uniformity of its growth and development of technology of industrial production of a new biomaterial.

In accordance with the invention of the technical result is achieved by the method for obtaining biologically active biomaterial-based natural compound. The difference is that as a natural compound selected biomass () Kombucha received incubation in aerobic conditions on a nutrient medium of a solution of sugar in the infusion of tea at room temperature for 14-160 days, from cultural liquid extract obtained and then dried at temperatures of 55 C up of biomaterials with a mass fraction of dry substances of more than 70%of mass.

The essential difference of the proposed method is to use the new biomass Kombucha (hereinafter ) to produce fundamentally new and environmentally friendly homogeneous biomaterial. Growing is conducted in a traditional setting. The growth of determined depending on the receipt of adequate quantity and properties of the products obtained. Also the significant difference is to bring the level of humidity extracted to the required level of product humidity (up to mass fraction of dry substances), which gather further manufacturing of this biomaterial, namely, synthetic leather, insoles, antibacterial plaster, biologically active food supplements (absorbent), used as a filler in the manufacture of medicines, food industry - as an additive in bakery and confectionery products (to slow , reducing caloric), as the stabilizer of food emulsions, to improve the fluidity of the product, and as an agent to reduce thickening properties, such as starch.

The technical result is achieved by the fact that the incubation carried out at a temperature of 25 deg C-30 C Thus receive more uniform growth .

The technical result is achieved by the fact that the nutritional environment is based on the condition that one liter of nutrient medium varies from 10 g to 90 g of sugar and 0.22 g to 1.5 grams of tea. Thus receive more high-quality biomass, namely, accelerated its growth and increases uniformity of its growth.

The technical result is achieved by the fact that of biomaterials as a basis of manufacture of bactericidal patch dried to a mass fraction of dry substances of more than 70% of mass up to 75%of mass.

The technical result is achieved by the fact that of biomaterials as a basis the manufacture of synthetic leather, insoles dried to a mass fraction of dry substances of more than 70% of mass up to 80%of mass.

The technical result is achieved by the fact that to get biomaterial as the basis of production of biologically active food supplements (absorbent) dried to a mass fraction of dry substances of more than 90 wt.%.

At the analysis of patent materials and technical literature we have not identified any materials discrediting invention.

Technological implementation of the proposed invention is based on the known developed by the applicant and the authors of the basic technological processes used in production (see including the production of the drink Kombucha by the patent RU2153816 of 06.10.1999). The proposal meets the criteria of the «industrial applicability».

In accordance with the invention of the technical result is achieved by a biologically active biomaterial-based natural compound. The natural connection is biomass () Kombucha received its incubation in aerobic conditions on a nutrient medium, representing a solution of sugar-containing product in the infusion of tea, and subsequently dried up to the content of mass fraction of dry substances of more than 70%of mass.

The technical result is achieved by the fact that at room temperature for 14-160 days.

The essential difference of the invention is a proposed new environmentally friendly homogeneous biomaterial for what used a new biomass - Kombucha (hereinafter ). Incubation is conducted in a traditional setting. The growth of determined on the basis of the number of necessary biomass. Also the significant difference is the level of humidity is required for products that are going to produce further from this biomaterial, namely, synthetic leather, insoles, antibacterial plaster, biologically active food supplements (absorbent), used as a filler in the manufacture of pharmaceuticals, and the food industry - as an additive in bakery and confectionery products (to slow , reducing caloric), as the stabilizer of food emulsions, to improve the fluidity of the product, as an agent to reduce thickening properties of starch.

The technical result is achieved by the fact that incubated at a temperature of 25 deg C-30 C will get a more uniform growth .

The technical result is achieved by the fact that the nutrient medium was compiled proceeding from the condition that one liter of nutrient medium varies from 10 g to 90 g of sugar and 0.22 g to 1.5 grams of tea. Thus receive more high-quality biomass, namely, accelerated its growth and increases uniformity of its growth.

The technical result is achieved by the fact that of biomaterials as a basis of manufacture of bactericidal patch obtained biomaterial with a mass fraction of dry substances of more than 70% of mass up to 75%of mass.

The technical result is achieved by the fact that of biomaterials as a basis the manufacture of synthetic leather, insoles received biomaterial with a mass fraction of dry substances of more than 70% of mass up to 80%of mass.

The technical result is achieved by the fact that to get biomaterial as the basis of production of biologically active food supplements (absorbent) received biomaterial with a mass fraction of dry substances of more than 90 wt.%.

Example. For biomass culture Kombucha (hereinafter ) used a pure culture of Kombucha Medusomyces Gisevii, which is a natural symbiosis strains of yeast and bacteria deposited in the all-Russian collection of industrial microorganisms FSUE research Institute of genetics, and put it in a nutrient medium. Is used the Nutrient medium prepared from the infusion of tea and sugar. To 100 litres of water consumption of tea 40 approximately 0.5 g and 1 kg of sugar Tea leaves and water boiling water, preferably not below the temperature of 98°±0.5 degrees C to withstand 20 approximately 0.5 min, In a nutrient medium made in the ratio of the number of and nurturing environment as 1:50. The culture of tea fungus grown at a temperature of 27 degrees to + / - 2 degrees C-30o C in the conditions of oxygen (in the open vessel) within 60 approximately 0.5 days (the period of growth depends on the amount of biomass). Growth speed can be increased by reducing the light and the absence of forced vibrations.

Next dried in air for 5 days. When this was achieved mass fraction of dry substances 74±1 wt.%.

To obtain a greater level of moisture is dried for two days, received mass fraction of dry substances 71±1 wt.%.

This biomaterial can serve as a basis for further manufacture of bactericidal patch.

To obtain a lower level of humidity used the forced extraction of liquid under the pressure and then dried in a drying chamber, receiving mass fraction of dry substances 78±1 wt.%.

This biomaterial can serve as a basis for further manufacture or leatherette, or Shoe inserts.

Depending on the modes of drying, time and weight press receive the required mass fraction of dry substances, including more than 80% of mass and more than 90%of mass. Perhaps a combination of air drying (e.g., 7 days) and forced.

Biomaterial with a mass fraction of dry substances of more than 90 wt.% is the basis for preparation of biologically active food supplements (absorbent). His grind in special plants up to the required level. Then, the finished grinding possible to package or tablet.

Biomaterial from biomass Kombucha with properties close to natural cellulose, found in the form of the natural component in food. is non-toxic and completely harmless. Side effects and there are no contraindications. effect on the human body in three ways: mechanical, sorption and vitamin. In the stomach absorbs liquid, swells, creating a feeling of satiety, and eliminates the feeling of hunger. In the small intestine mechanically cleans the mucous membrane of the «mud» film - broom to the intestine. In addition, throughout the entire gastrointestinal tract Sorbs (absorbing) harmful substances, heavy metals, radionuclides etc can be used in case of poisoning (instead of activated coal); in order to normalize the digestion; diabetes; atherosclerosis, ischemic heart disease, gastrointestinal diseases; formation of stones in the gastric bladder, kidneys and other, as well as for routine annual cleansing the body and getting rid of excess weight. keeps the whole range of the studied useful actions cultural liquid Kombucha, as it is its derivative.

can be used as filler in the manufacture of drugs. Tablets of easy release of medicinal substances, a time of disintegration of pills. Chemical and physical stability , excellent compressibility tablets allow to use it in the production of tableted dosage forms. Perfectly combine with natural extracts. can serve as one of the main ingredients of medicinal ointments.

In the food industry can be used as an additive in bakery and confectionery products (increases the storage time, slows aging, reduces the calorific value); as a stabilizer, food emulsions; improver fluidity of the product; agent, reducing properties of starch.

Thus, for the first time obtained biologically active additive to food from biomass () Kombucha with a large range of useful actions on humans, animals.

New biomaterial will take its rightful place within the range of currently available on the Russian market of biologically active additives, as healthy, natural, high quality product, the analogue of which yet were not.

Industrial applicability

The invention relates to the food industry, for example, using biomass as a preservative of food products, drinks, pastries, confectionery products, the pharmaceutical industry for obtaining of a filler in the medicines, to receive biologically active additives, industrial production , medical insoles etc

1. The method of obtaining biologically active biomaterial-based natural compounds, characterized in that, as a natural compound selected Kombucha received its incubation in aerobic conditions on a nutrient medium, representing a solution of sugar-containing product in the infusion of tea at room temperature for 14-160 days, from the culture medium extract obtained and then dried at a temperature of 55 C up to the content of mass fraction of dry substances of more than 70%by weight.

2. The method according to claim 1, characterized in that the incubation carried out at a temperature of 25-30°C.

3. The method according to claim 1, wherein the nutrient environment is based on the condition that one liter of nutrient medium varies from 10 g to 90 g of sugar and 0.22 g to 1.5 grams of tea.

4. The method according to claim 1, characterized in that of biomaterials as a basis of manufacture of bactericidal patch dried to a mass fraction of dry substances over 70 and up to 75%by weight.

5. The method according to claim 1, characterized in that of biomaterials as a basis the manufacture of synthetic leather insoles dried to a mass fraction of dry substances and more than 75 to 80 wt.%.

6. The method according to claim 1, characterized in that of biomaterials as a basis of manufacture of biologically active food supplements (absorbent) dried to a mass fraction of dry substances of more than 90 wt.%.

7. Biologically active biomaterial, characterized in that it is obtained according to claims 1 to 6.