Antimicrobial activity of robinia seed essence

FIELD: medicine, pharmaceutics.

SUBSTANCE: invention refers to pharmaceutical industry, specifically to an agent showing antimicrobial activity.

EFFECT: agent possessing antimicrobial action and representing robinia (Robinia pseudaacacia L) seed essence prepared by collecting the seeds in October and extracting them three times in petroleum benzene under certain conditions.

2 tbl, 1 ex

 

The invention relates to medicine, medical industry and can be used in particular to the use of essential oils from seeds of Robinia pseudoacacia (Robinia pseudaacacia L.) as a means possessing antimicrobial activity.

A method of obtaining oil protivopolozhnogo [RF patent №2416425], containing in its composition the fatty oil of sea-buckthorn and additionally fatty Tamanu oil and wheat germ, as well as essential oils of marjoram, geranium, cedarwood, marjoram and other plants.

A method of obtaining the composition [RF patent №23 72096]possessing antifungal, antimicrobial and virulizin effect, characterized in that it contains as the base oil, aqueous-alcoholic extracts and essential oil of monarda fistular, propylene glycol extracts of pine and poplar, essential oils of peppermint, Basilica and other Disadvantages of these methods are that they consist of many components, in addition, the simultaneous extraction of all components of the plant materials collected at different times, as they ripen, forced to keep the number of components in anticipation of the other, which leads lose their medicinal properties.

A method of obtaining the extraction of essential oil from green conifers [RF patent №2396309], characterized in that the oil exhibits antibacterial properties. SP who own shredding the wood green conifers and extraction of the liquefied carbon dioxide at a temperature of 20-22°C and a pressure of 6-7 MPa. The drawback of this method lies in the complexity of the technological operations for the extraction of essential oils and the use of expensive equipment.

A method of obtaining biologically active substances [RF application No. 94025595]having antimicrobial, phagocytic, mitotic and antioxidant activity, including the preparation of the ether concentrate by mixing peppermint oil and/or penhelig, caraway, anise, dill, coriander, Bergamote, lemon, orange, eucalyptus, San taly, ilang-ilang, nutmeg-sage and other oils and aqueous extract of the herb wormwood, peppermint, buds of Scots pine, licorice and other plants. However, this method has several disadvantages, namely, the low degree of extraction of biologically active substances from plant material in water, the technological difficulties of the process of fine filtration of the obtained extract, due to the large volume of filtered mass, consisting of many components that can affect the properties of the drug. In addition, the simultaneous extraction of all components of the plant materials collected at different times, as they ripen, forced to keep the number of components in anticipation of the other, which leads to the loss of their properties.

A method of obtaining essential oils of Lough is the anise [RF application No. 2009143766] the extraction method with water vapor, containing methylchavicol, as well as substances with antifungal, antibacterial and disinfectant action. The main disadvantage of this method is that Lavant anise is introduced into Russia by the plant and the technology of its cultivation is time consuming (regular watering plants, making feeding and pre-sowing cultivation of the earth).

A method of obtaining essential oils from Artemisia [RF patent №2356567] method partictilarly, has antibacterial, disinfectant, antiseptic, anti-inflammatory, wound healing and burns effect. However, these extracts and essential oils may not be counterparts, as obtained from other plants.

The problem solved by the invention, is the study of antimicrobial activity of essential oils from plant material false pseudoacacia (Robinia pseudaacacia L.). The problem is solved with the help of essential oil from seeds of Robinia pseudoacacia collected in the month of October and obtained by the method of three times of extraction with petroleum ether by heating for 45 minutes at a volume ratio of solvent to raw material 3:1.

Essential oil of Robinia pseudoacacia by finding in it the complex of biologically active substances shows appreciable antimicrobial activity to Shir the who series as gram-negative, and gram-positive microorganisms.

The method is based on the study of antimicrobial activity was the study of the influence of active substances essential oils false on the respiratory system of microorganisms. The level of respiratory or its suppression was observed in the degree of consumption of glucose indicator methylene blue. Antimicrobial activity was determined by the method of serial dilution in 0.5 ml of nutrient broth [Gerhard F. Methods of General bacteriology. M., 1983. 612 C.]. As the test cultures used strains of pathogens of nosocomial infections: Escherichia coli SC, Klebsiella 204, 1919 Bacillus mesentericum-3, Pseudomonas aeruginosa 165, Staphylococcus aureus 209, Proteus vulgaris, Klebsiella pneumoniae, Acinetobacter calcoaceticus, Staphylococcus aureus, Staphylococcus aureus MRSA. Antimicrobial activity of the essential oil of Robinia pseudoacacia illustrated by the following example.

Example.

Pure bacterial cultures grown on nutrient agar medium, placed in a thermostat at 37±1°C, washed with environment after 18-20 h 0,89% solution of sodium chloride. Preparing a suspension according to the turbidity standard 1·109cells/ml (10% opacity). To obtain the appropriate dilution of the microbial suspension was preparing a series of seven consecutive breeding. After the definition of "working dose of the test culture was titrated essential oil by twofold dilution in a volume of 0.5 ml meat-peptone broth. Lastly the tube I in the control series did not contain essential oils and served as a control dehydrogenase activity of the cells in the test culture. In the microbial load in vitro corresponded to "working dose of the test culture. Tubes with essential oil and test cultures were placed on a 24 h exposure at 37°C, and then made the indicator methylene blue with glucose and meat-peptone agar, the contents of the tubes were mixed and incubated for one hour at a temperature of 37±1°C. the Result was considered by the color of the nutrient medium, if the indicator is bleached, it showed the absence of suppression, if the color has not changed, it testified to the blocking of the respiratory enzymes in cells of the test cultures of the active components of the essential oil. As the comparison drug used gentamicin sulfate. The results were subjected to statistical analysis using t-student test.

The results of the study of antimicrobial activity of the essential oil of Robinia pseudoacacia are shown in tables 1 and 2.

Thus, the essential oil from seeds of Robinia pseudoacacia (Robinia pseudaacacia-L), shows an appreciable antimicrobial activity against a wide range of both gram-negative and gram-positive microorganisms.

Application.

Table 1
Antimicrobial activity of essential oil from seeds ro is the turn of pseudoacacia against strains of gram-negative bacteria
The analyzed sampleMBC (µg/ml) against
E.coli SKKlebsiella 204Pseudomonas aeruginosa 165Proteus vulgarisKlebsiella pneumoniaeAcinetobacter calcoaceticus
Robinija pseudoacacia2055*205*10
Gentamicin sulfate2.51.250.63*2.51.251.25
*Differences between repetitions significant at p=0,95

Table 2
Antimicrobial activity of essential oil from seeds of Robinia pseudoacacia against strains of gram-positive bacteria
The analyzed sampleThe PC (μg/ml) against
In 1919 Bacillus Mesentericum-3Staphylococcus aureusStaphylococcus aureus 209Staphylococcus aureus MRSA
Robinija pseudoacacia5*0,630,63*5
Gentamicin sulfate1.250.630.63*0.63
*Differences between repetitions significant at p=0,95

Means having antimicrobial activity, which represents the essential oil from seeds of Robinia pseudoacacia (Robinia pseudaacacia L.)obtained by collecting seeds in the month of October, three times of extraction with petroleum ether by heating for 45 min at a ratio of 1:3.



 

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