Sorbent-ameliorant for cleaning oil-contaminated soil
SUBSTANCE: three types of clay of different chemical composition is used with addition of molasses and Linex biopreparation in the following ratios, wt %: Dialbeculit clay -38-40; Irlit clay 1 - 28-32; Irlit clay 7 - 16-20; molasses - 8-12; Linex biopreparation -2-4. Adding such a composition to the soil reduces the amount of oil pollutants by 72%.
EFFECT: low toxicity of soil and expenses on amelioration.
1 tbl, 1 ex
The invention relates to the reclamation of soils and can be used for remediation of soils contaminated with oil.
Known improver consisting of clay and humus layer with the addition of coarse sand (patent No. 2253225 from 10.06.2005, IPC A 25/00, A01B 79/02).
Known improver is created at high cost, so as to accommodate sorbing substances perform sloping of the trench to a depth of 2-3 m
Known improver to reduce the pollution of the land of petroleum products by culture plants of phytoremediation that handle the strains of microorganisms (patent 2403102 from 10.11.2010, IPC VS 1/10).
However, to implement technical solutions require a significant amount of inoculum, which also increases costs.
Known composition for soil reclamation "Sorbex" on an organic basis with the addition of mineral components, the organic bases used sapropel, and as mineral components - zeolite and alumina in the following ratio of components, wt. %: sapropel 60-70, zeolite 23-27, alumina 8-11 (patent No. 2019107 from 27.11.1995, IPC SC 17/00).
Known composition mnogosotletney, and in the extraction of sapropel violation occurs landscape, disrupting the ecology of the area.
The closest composition to the present invention is a sorbent-improver, sustasis bentonite clay (36%), ash mixture (47%), blue clay (17%), (patent 2303623 27.07.2007, IPC SC 17/00).
A disadvantage of the known improver of the prototype lies in the high costs as to the place of pollution need to deliver ash mixture in large quantities and blue clay, located in remote locations.
The technical result is lower cost and toxicity of the soil.
The technical solution is that use ceritadewasa clay of local origin, containing a set of macro - and micronutrients with the addition of waste of sugar production molasses and biological Linex in the following ratio of components, %:
clay vallecula - 38-40;
clay Irlit 1 - 28-32;
clay Irlit 7 - 16-20;
molasses - 8-12;
biologic Linex - 2-4.
The advantage of the proposed sorbent is that zeolite-containing clay of local origin and are located in the tributaries of the Terek river, in the upper and lower parts having different chemical composition.
Clay vallecula consists of silicon (46,5%), iron (7,1%), potassium (1,1%), calcium (37%), cobalt (0,1%), zinc (1,1%), Nickel (1,7%), phosphorus (1.7 percent). The reaction medium alkaline (pH 9,1).
The Irlit 7, unlike valleculae contains: silicon 54%, aluminum - 16%, iron 4%, sulfur - 2,5%, potassium - 2%, essential elements (copper, cobalt, molybdenum, zinc, selenium) in the range of 0.1-0.9%. eacce medium acid (pH 3,8).
The Irlit 1 consists of silicon (54%), aluminum (28%), iron (7%), sulfur (2%), calcium (3%), magnesium (1.7 percent), manganese (1,7%), potassium (2,1%), sodium (1,1%), sulfur (2,0%), selenium (0,8%). The reaction medium is neutral. All clays have a high sorption properties due to the high content of silicon (46-54%), ability to absorb moisture and other substances.
The combination of clay with different pH equalize the effect of trace elements. Silicon contributes to the release of plant phosphorus due to displacement of its ions from difficultly soluble phosphates in the soil, while the silicic acid anions are able to block free carbonates in the soil solution, which reduces retrogradely soluble phosphates. Siliceous clay increase the mobility of phosphation in the soil. When the soil pollution with oil decreases the mobility of phosphorus, therefore making clays with a high content of silicon recovers phosphorus compounds, thereby reducing the amount of oil in the soil.
Clay vallecula differs from other known zeolite-containing substances lighter specific gravity (1.4 to 1.45 g/cm3), which makes the peculiarity of its mineralogical composition (the presence of a large number of hydrolog). Vallecula contains a noticeable amount of water-soluble salts, closer this index is to low mineralized sludge is sulfide mud, with high sorption properties.
Therefore, as part of the proposed mix these clays predominate (38-40%). Given the composition of other types of clays (Irlit 1 and Irlit 7), their reaction environment and the complex of microelements, supplementing the clay vallecula in the proposed proportions, justified dosage of each type.
Biologic Linex is a combination drug, which consists of 3 components of the natural microflora (Lactobacillus acidophilus, Bifidobacterium infantis, Enterococcus faecium), resistant to chemical agents.
This drug is usually used for alignment of the intestinal microflora of living organisms. Lactic acid bacteria that are part of Linex, support and regulate physiological process of the microorganisms in the soil.
Made molasses waste of sugar manufacture, contains 20-25% water, about 9% of the nitrogen compounds, mainly amides, 58-60% carbohydrates, mainly sugar and 7-10% ash. Used as carbohydrate food. In this object as a means of power for lactic acid bacteria preparation Linex.
The ability of clay to retain moisture, warmth provide favorable conditions for the development of microorganisms in the soil. Contained in clays sulphur and other isnonnegative elements provide the activity of anaerobic microorganisms type Linex.
The structure for land reclamation prepared to follow the way:
Clay vallecula (a tributary of the Terek - Urdon) in the amount of 3.8-4.0 t/ha was mixed with the Irlit 1 (2,8-3,2 t/ha), with the Irlit 7 (1.6 to 2.0 t/ha) - origin of Alagir district river Fiagdon. Molasses in the amount of 0.8-1.2 t/ha was mixed with biological Linex in the amount of 0.2-0.4 t/ha just before making.
Before soil application of crushed clay was placed in fertilizer planter mixed with molasses and Linex and brought directly into the soil to a depth of 10-15 cm
The results of the experiments after a single make that amount of sorbent (9-10 t/ha) showed significantly decreased the oil content in the soil. The results of the experiments are summarized in table (0-20 cm soil layer after 6 weeks after application).
|Variants of experiments||Hydrocarbons, kg/m2||Concentration neftezagryazneny substances, g/kg||Reduction neftezagryazneny substances, %|
|Pollution with oil products (control)||2048||65,0||-|
|Clay Irlit 1/td>||1756||52,4||to 19.9|
|Clay Irlit 7||1814||56,8||13,6|
|A mixture of 3 clays||918||36,2||44,4|
|A mixture of 3 clay + molasses||826||28,5||56,2|
|A mixture of 3 clay + Linex||586||25,4||60,3|
|The proposed option (mix 3 clay, molasses + Linex)||350||18,2||72,0|
Standard AFNOQ 90114 level of hydrocarbons should not exceed 500 mg/kg of soil. As follows from the table, the proposed option provides a reduction of hydrocarbons up to 350 mg/kg the number of neftezagryazneny substances is reduced by 72%.
Therefore, the introduction of various types of clays mixed with bacteria and molasses provides the reclamation of soils contaminated with oil.
Sorbent-improver for cleaning oil-polluted lands, consisting of Gleni the mentioned mixtures,
characterized in that it contains
clay Vallecula content, wt.%: Si - 46,5, CA - 37, Fe - 7,1, 1,1, 0,1, Zn - 1,1, Ni - 1,7, P - 1.7 and pH of 9.1,
clay Irlit 1 content, wt.%: Si - 54, Al - 28, Fe - 7, S - 2, Sa - 3, Mg - 1,7, Mn - 1,7, K - 2,1, Se - 0,8, Na - 1,1-neutral pH and
clay Irlit 7 content, wt.%: Si - 54, Al - 16, Fe - 4, S - 2,5, K - 2, essential elements copper, cobalt, molybdenum, zinc, selenium is in the range of 0.1 to 0.9 with an acidic environment, pH 3.8,
waste of sugar manufacture of molasses and
biologic Linex in the following ratio, wt.%:
|clay Irlit 1||28-32|
|clay Irlit 7||16-20|
SUBSTANCE: method includes preparation of the inoculum of cells Streptomyces sp. MT246 (All-Russian Collection of Microorganisms Ac-2618D). Preparation of the nutrient medium containing one or more sources of carbon, nitrogen, metal ions in the form of soluble salts with fractional feeding of the carbon source during the productive stage. At that, as an additional carbon source the nutrient medium contains rhamnose, and a necessary component of the nutrient medium - MnS04 and sorbent from the group of amberlites XAD. Adding of the inoculate of cells Streptomyces sp. MT246 (All-Russian Collection of Microorganisms Ac-2618D) to the nutrient medium together with rhamnose and MnSO4 in a predetermined ratio and incubating of the medium at pH 7.0-7.5, temperature 25-35°C for 7-10 days. Separation of the residue is carried by centrifugation. The resulting residue is added to ethanol at a predetermined ratio, followed by extraction of tacrolimus at 30 for 2 hours and centrifuging to obtain the extract comprising tacrolimus.
EFFECT: invention enables to increase the yield of tacrolimus.
9 cl, 6 ex
SUBSTANCE: invention relates to a synbiotic preparation containing N-acetyl-lactosamine and/or an oligosaccharide containing N-acetyl-lactosamine and a probiotic strain Lactobacillus sp. The oligosaccharide containing N-acetyl-lactosamine is lacto-N-tetraose or lacto-N-neotetraose. The probiotic strain Lactobacillus sp. is Lactobacillus rhamnosus ATCC 53103, Lactobacillus rhamnosus CGMCC 1.3724, Lactobacillus reuteri ATCC 55730 or Lactobacillus reuteri DSM 17938.
EFFECT: synbiotic preparation is used in infant nutritional composition as well as in production of a nutritional composition for preventing or as a medicinal agent for preventing or treating pathogenic infections of the gastrointestinal tract and upper respiratory tracts.
6 cl, 1 tbl, 1 ex
SUBSTANCE: method includes extraction of DNA from strains B.pertussis, performance of a polymerase chain reaction with simultaneous introduction of specific primers PF-PR for production of amplicons of a fragment of a promotor of pertussis toxin ptxP, restriction of produced amplicons with endonucleases Kpnl and Mlul and electrophoresis of restriction products in agarose gel. Differentiation of strains B.pertussis is carried out by comparison of size of amplicons of DNA fragments of promotor ptxP of investigated strains B.pertussis with reference samples of DNA strains B.pertussis.
EFFECT: invention may be used for accelerated detection of epidemic strains with modified genetic structure during microbiological and molecular-genetic monitoring of Bordetella pertussis strains and for further development of promising diagnostic and prevention preparations on their basis.
SUBSTANCE: strain is deposited under the number RCAM 00876 in the collection of All-Russia Research Institute of Agricultural Microbiology of the Russian Agricultural Academy. The strain Bacillus subtilis 8A has high fungicide activity against phytopathogenic activity against phytopathogenic fungi and bactericide activity against phytopathogenic bacteria, and also growth-stimulating activity relative to various crops.
EFFECT: strain may find application in agriculture as an efficient agent to increase productivity of plants and their protection against phytopathogenic microorganisms.
FIELD: food industry.
SUBSTANCE: invention relates to a food product with extended storage life and to usage of probiotic spores and/or resting cells as a probiotic ingredient in the said product. The product is chosen from the group containing milk products, fruit juices and fruit beverages. The probiotic spores are chosen from the group containing bacterial, yeast and fungous spores. The resting cells are chosen from bacterial, fungous and/or yeast and/or their mixtures. The produced is stored under uncooled conditions during a long period of time by way of probiotic bacteria spores inclusion.
EFFECT: probiotic organisms contained in fruit juices and beverages remain viable during a longer period of time in comparison with live probiotics.
23 cl, 9 ex
FIELD: food industry.
SUBSTANCE: invention relates to farm biotechnology and may be applied during preparation of fodders for farm animals and birds. The biopreparation contains microbial mass of Bacillus cereus 1-79 bacteria strains. The strain consists of microbial mass of Bacillus cereus 1-79 spore-forming bacteria with titre equal to 3.5×109 CFU/g - 4.0×109 CFU/g, suspended in a nutrient medium. The strain is deposited in the All-Russian State Collection of Microorganism Strains under Registration Number of Strain Bacillus cereus 10.09.63 DEP and stored in microorganisms collection LLC BIOTROF.
EFFECT: invention allows to enhance fodders assimilation due to usage of a new biopreparation with high probiotic activity.
8 tbl, 3 ex
SUBSTANCE: culture fluid of producer strain is concentrated on hollow fibers truncating macromolecules with a molecular weight higher than 15 kDa. The cell concentrate is added to dry NaCl in a final concentration of 0.5 M. Mixed on a shaker for 20 min. The suspension is centrifuged for 15 min at 10000 g and the supernatant is separated, the supernatant pH is made up to 3.0 by four-molar solution of HCl. The suspension is centrifuged. The residue is added to water in a volume of 0.1% of the initial volume of the culture fluid, the residue is suspended and alcohol is added in amount of 0.1% of the initial volume of the culture fluid. Ethanol or propanol or isopropanol is used as alcohol. Exposed for 30 minutes at 0°C and centrifuged. The alcohol is removed from the solution by evaporation. The peptide fraction is purified. Water is added to 0.1% of the initial volume of the culture fluid, active carbon is added in an amount of 0.5% w/v (v - water volume). Centrifuged removing impurities adsorbed on the carbon. The aqueous solution is passed through the membrane truncating macromolecules with a molecular weight more than 10 kDa.
EFFECT: invention enables to accelerate preparation of bacteriocin and to increase the degree of purification of the resulting product in a yield of 90% of the total activity in the culture fluid.
1 dwg, 1 tbl, 4 ex
SUBSTANCE: fermentation medium for production of recombinant proteins using methanol inducible fungi of species Pichia pastoris is characterised by maintained concentration of urea or its derivatives in the range from about 0.3 M to about 1 M. The medium contains per liter of water basic salts in the following amounts: orthophosphoric acid (85%) from 2.67 to 133.5 ml, calcium sulfate from 0.093 to 4.65 g, potassium sulfate from 1.82 to 91 g, magnesium sulfate-7H2O from 1.49 to 74.5 g, potassium hydroxide from 0.413 to 20.65 g, glycerol from 4 to 200 g, and microelements per liter of water in the following amounts: copper sulfate-5H2O from 0.6 to 30 g, sodium iodide from 0.008 to 0.4 g, manganese sulfate-H2O from 0.3 to 15 g, sodium molybdate-H2O from 0.02 to 1 g, boric acid from 0.002 to 0.1 g, cobalt chloride from 0.05 to 2.5 g, zinc chloride from 2 to 100 g, sulphate divalent iron-7H2O from 6.5 to 325 g, biotin from 0.02 to 1 g, sulfuric acid from 0.5 to 25 ml. The method for production of recombinant proteins comprises the stage of reproduction of methanol inducible fungi of species Pichia pastoris, and the stage of recombinant protein expression using the said fermentation medium in which feeding with methanol is carried out at a rate of about 6 g/l/hr to about 20 g/l/hr.
EFFECT: increased yield of target products.
11 cl, 27 dwg, 16 ex
FIELD: medicine, pharmaceutics.
SUBSTANCE: bifidobacterial and lactobacillary consortium contains Bifidobacterium bifidum OV-19, B. bifidum 791, B. longum OV-20, B. longum"Я"-3, B. adolescentis "ГО"-13, Lactobacillus helveticus "К3ш24", L. helveticus NK-1, L. casei KHM-12; it is used for preparing bacterial preparations and dietary supplements for correcting the microflora in the individuals of fourteen and older. The consortium is high-technology; it stores a biomass on nutrient mediums over a short period of time with a high concentration of probiotic microorganisms, possesses the acid-forming and antagonist properties in relation to the pathogenic and opportunistic microflora. The consortium is prepared by the co-culture of the strains including the length of an exponential growth phase of each of them whereby a priority of the nutrient medium inoculation of the specific composition is determined.
EFFECT: using the consortium as a part of the bacterial preparation for treating the dysbiotic gastrointestinal conditions in the individual of fourteen and older, and the dietary supplement provides the more effective and fast correction of the child's gastrointestinal microflora.
4 cl, 10 tbl, 3 ex
FIELD: medicine, pharmaceutics.
SUBSTANCE: bifidobacterial and lactobacillary consortium contains Bifidobacterium bifidum 19, B. bifidum 791, B. longum B379M, B. longum OV-20, B. breve OV-12, L .helveticus "К3Ш24", L. helveticus NK-1, L. casei KHM-12; it is used for preparing bacterial preparations and dietary supplements for correcting the microflora in the children aged from three to fourteen. The consortium is high-technology; it stores a biomass on nutrient mediums over a short period of time with a high concentration of probiotic microorganisms, possesses the acid-forming and antagonist properties in relation to the pathogenic and opportunistic microflora. The consortium is prepared by the co-culture of the strains including the length of an exponential growth phase of each of them. Thereby a priority of the nutrient medium inoculation of the specific composition is determined.
EFFECT: using the consortium as a part of the bacterial preparation for treating the dysbiotic gastrointestinal conditions in the children aged from three to fourteen, and the dietary supplements, provides the more effective and fast correction of the child's gastrointestinal microflora.
4 cl, 10 tbl, 3 ex
SUBSTANCE: invention relates to soil and ground chemical propping compositions and can be used in agriculture against water and wind erosion, as well as in construction of roads and other earth structures. The disclosed aqueous soil and ground propping composition contains 0.90-4.62 wt % of a charged polyelectrolyte complex and 0.08-0.87 wt % of a salt of an alkali metal or ammonia. The charged polyelectrolyte complex in said composition is a product of reacting water-containing solutions of a cationic polyelectrolyte, which contains chloride and bromide counter-ions, and an anionic polyelectrolyte from a group which includes a sodium salt of carboxymethyl cellulose, an alkali metal or ammonium salt of a polyacrylic acid or polymethacrylic acid, taken in a ratio where content of charged links of one polyelectrolyte ranges from 51% to 60% of the content of charged links of the other polyelectrolyte.
EFFECT: compared to the closest counterpart, the disclosed composition provides efficient soil and ground propping from wind erosion while reducing content of salt in the composition from 1,5-3,7 wt % to 0,08-0,87 wt % and consumption of the composition from 1-2 l/m2 to 0,3-0,45 l/m2.
SUBSTANCE: invention relates to soil and ground chemical propping methods and can be used in agriculture against water and wind erosion, as well as in construction of roads and other earth structures. The method involves mixing aqueous solutions of an anionic polyelectrolyte and a cationic polyelectrolyte and a water-soluble salt. The polyelectrolytes are mixed with their total initial concentration ranging from 1 to 10 wt % in a ratio at which content of charged links of one polyelectrolyte is equal to 5-50% of the content of charged links of the other polyelectrolyte and concentration of at least one salt, selected from a group comprising an alkali metal salt, an ammonium salt, a calcium salt and a magnesium salt, ranges from 0.01 to 0.1 wt %.
EFFECT: low content of salt in the composition and lower consumption rate of the composition.
7 cl, 7 ex
SUBSTANCE: invention relates to soil and ground chemical propping compositions and can be used in agriculture against water and wind erosion, as well as in construction of roads and other earth structures. The composition contains a polyelectrolyte, a water-soluble salt and water. The polyelectrolyte is a charged polyelectrolyte which is a reaction product of aqueous solutions of a cationic polyelectrolyte and an anionic polyelectrolyte, selected from a group comprising a sodium salt of carboxymethyl cellulose, an alkali metal or ammonium salt of polyacrylic acid or polymethacrylic acid, taken in a ratio at which content of charged links of one polyelectrolyte is equal to 5-50% of the content of charged links of the other polyelectrolyte, and the salt is an alkali metal or ammonium salt, or a mixture of such a salt with a calcium or magnesium salt, with the following ratio of components, wt %: reaction product 0.91-9.91; water-soluble salt of aqueous solutions of the cationic and anionic polyelectrolytes 0.10-0.62; water - the balance.
EFFECT: low content of salt in the composition and lower consumption rate of the composition.
SUBSTANCE: invention relates to the field of recultivation of damaged soil in conditions of the Far North and may be used when restoring the soil and vegetation layer disturbed by industrial and economic human activities. The method of recultivation of damaged soil is described, including introduction into the ground of bentonite clay, seeds of perennial grasses, fertilisers, humic compounds and binder, and the bentonite clay is inserted as part of the waste mud, followed by stirring the top layer of soil. Then the fertiliser, humic compounds and seeds of perennial grasses are inserted, and then a binder is inserted, which is used as a solution of xanthan gum.
EFFECT: invention provides a more efficient process of recultivation, expansion of the scope of application on the surface of soils with different steepness.
SUBSTANCE: sapropel and natural loosener are ploughed into soil at the depth of 10-15 cm. Natural loosener is represented by siftings of open pits of construction materials, at the following weight ratio of components, %: sapropel 8-12%; siftings of pits of construction materials 13-32%; upper layer of clayey soil - balance.
EFFECT: increased bioproductivity of soils and protection of recultivated soils against wind erosion.
1 cl, 2 tbl
SUBSTANCE: invention relates to agriculture and may be used to reduce soil acidity, improve its structure and fertility. Method implies soil application of deoxidiser, which is a mixture of organic and mineral components with a deoxidising agent. Peat of 30-70% decay degree and pH 3.5-4.5 is used as organic component, borogypsum is used as mineral component and 53% alkaline solution (potassium hydroxide) is used as deoxidising agent. Ratio of components (kg per 1 tonn of resulting deoxidiser) is as follows: peat of 30-70% decay degree and pH 3.5-4.5 1200-1230, 53% potassium hydroxide solution 48-49, borogypsum 70-74. Deoxidising agent is applied in amount of 2-3 t per 1 ha.
EFFECT: reduced soil acidity, improved structure and fertility.
SUBSTANCE: binding composition for processing material of solid particles with formation of solid aggregated matrix is prepared including the following stages: blending of polar solvent and additional quantity of urea; addition of emulsified bitumen; addition of ureaformaldehyde precondensate; addition of other binding promotors in order to enhance bonding between the binding composition and solid particles; addition of sugar into weak organic acid; addition of weak organic acid mixed with sugar with formation of the binding composition; blending of binding composition with solid particles, which harden during over 30 minutes while mixing with above mentioned material of solid particles, besides weak organic acid is being chosen to ensure pH value of binding composition from 2.0 to 5.3.
EFFECT: binding composition was prepared for processing of materials of solid particles.
51 cl, 15 dwg, 3 tbl, 11 ex
FIELD: agriculture, in particular improvement of soil physical, biological and agronomic characteristics.
SUBSTANCE: claimed method includes introducing of carbonate-containing material into soil, wherein as said material bergmeal containing (mass %): calcite 13-19; zeolite 18-25; glauconite; and balance: clay and silicate material is used. Bergmeal is introduced into soil in amount of 1.5-5 t/hectare.
EFFECT: enhanced assortment of ameliorants; improved soil agronomic characteristics and productivity.
FIELD: production of filled gel-like composition with viscoelastic properties.
SUBSTANCE: claimed composition contains (vol.pts): 0.5-2.0 mass % sodium tetraborate aqueous solution 1-2; 1.0-8.0 mass % polyvinyl alcohol aqueous solution 1-5; 1.0-8.0 mass % carboxymethyl cellulose aqueous solution 1-5 or 1.0-8.0 mass % polyacrylamide aqueous solution 1-2; and filler 1-3. Composition has dynamic viscosity of 642-2467.87 mPa.s; elasticity modulus of 61.12-81.12 N/m2, and adhesion of 53.76-86.79 N/m2. As filler sand with particle size of 0.23-0.5 mm; lavsan or propylene fiber of 70-100 mum in diameter and filament length of 3-6 cm, or Teflon granule at most 1 mm in diameter. Composition of present invention is useful in production of petroleum industry, oil line purification, as anticorrosion, biocide and wear resistant coatings.
EFFECT: new viscoelastic composition of improved quality.
SUBSTANCE: invention relates to the field of remediation, biotechnology and waste utilisation of brewing industry. The natural highly porous aluminosilicate adsorbent-meliorant and slowly released organic fertiliser are applied in the soil, which are used as the waste fossil flour formed as a waste during beer filtration, dried brewers' grains formed during the mash filtration, mixed with the waste fossil flour in the ratio of 1-5:1-2, or mixed with excessive beer yeast sedimentating at the end of the main fermentation, and waste fossil flour in the ratio of 1-5:0.1-1:1-2. Then the resulting mixture is applied into the oil-contaminated soil and loosening is carried out, the procedure is repeated in a month.
EFFECT: method enables to increase the biological activity of the soil, to increase the content of useful chemical agents in it, to improve the structure and water permeability and increase the effectiveness of cleaning soil.