Fibroblast growth agent

FIELD: medicine.

SUBSTANCE: invention refers to cosmetic industry, namely a fibroblast growth agent. The fibroblast growth agent containing yeast extract and safflower extract as effective ingredients, wherein the yeast extract is prepared from yeast cultured in a nutrient culture medium containing glycosaminoglycan and which is subjected to ultraviolet radiation and hydrogen peroxide treatment. A topical skin preparation containing the fibroblast growth agent. A method for fibroblast growth which uses the yeast extract and safflower extract. An anti-age skin product containing the yeast extract and safflower extract.

EFFECT: fibroblast growth agent of the yeast extract and safflower extract has an evident effect of the fibroblast growth.

7 cl, 1 dwg, 3 ex

 

DESCRIPTION

The technical field to which the invention relates.

The present invention relates to a tool for the growth of fibroblasts. In particular, the present invention relates to a tool for fibroblast growth that can accelerate the growth of the age fibroblasts. The present invention also relates to the external preparation for skin, where the specified product contains the means for the growth of fibroblasts.

Background of invention

In recent times, keeping the skin healthy and beautiful becomes of particular concern, regardless of age or gender. The skin consists mainly of three layers, namely the epidermis, dermis, or skin, and hypodermal tissue. Of these three layers, the dermis is especially important for maintaining the structure of the skin. The dermis is a connective tissue having a structure in which, basically, fibroblasts, collagen fibers, elastic fibers (elastin) and so on, are distributed in a complex three-dimensional order. Proteins, such as collagen, which are the fibers are produced by fibroblasts. Fibroblasts are able to maintain the balance of synthesis and degradation of proteins and maintain connective tissue in a particular state. Due to this, supported by the strength, elongation and elasticity of the skin.

In connection with the action of some inside the x factors such as aging, and external factors such as UV radiation and reactive reactive oxygen level of the connective tissue in the dermis decreases and weakens its function, which in the initial state, support the skin, such as airway, flexibility and hydration. As a result, there are various skin problems such as wrinkles, sagging and reduce the softness and elasticity of the skin. It is believed that important determinants of the above symptoms of aging are reduced number of fibroblasts in the dermis of the skin and weakening of the functions of fibroblasts in the dermis of the skin, while also reducing the number of matrix fibers which are produced by fibroblasts. In this regard, to obtain anti-aging external preparation for skin having the ability to prevent and reduce skin aging, you should take a search for a particular component, created on the basis of a mixture with the ability to accelerate the growth of fibroblasts.

For example, in papers 1 and 2 in the list of references is reported that the plant extracts, such as Lens esculenta, Piper longum, Centella asiatica, Ocimum sanctum, Ocimum tenuiflorum and Ocimum album is able to activate the growth of fibroblasts and that the substances described above can be mixed with anti-aging drugs, etc.

However, when the aging ability is of fibroblasts to cell proliferation decreases with aging, so that there is a decrease in the rate of mitosis of fibroblasts. In the case of traditional tools used for fibroblast growth problems associated with the fact that these traditional tools for fibroblast growth being effective for young fibroblasts, may not always provide sufficient growth-stimulating effect in the case of senescent fibroblasts. Therefore, there is an expressed need in the development of new substances that can enhance the growth of senescent fibroblasts.

It is known that yeast extract has the ability to enhance the production of collagen (see patent 3 in the list of references), to provide a moisturizing effect (see patent 4 in the list of references), activate cells and increase production of hyaluronic acid (see patent 5 in the list of references). Yeast extract is mixed with external preparations for the skin, such as cosmetic preparations.

According to the concepts of Chinese medicine, it is known that the extract of safflower able to improve the properties of the blood. From an aesthetic purpose, the extract of safflower is used to restore the body with an excess of "cold" (improving blood flow in the outlying areas of the skin), to mitigate the severity prevage lesions of the skin and for the treatment of atopic dermatitis (see patent 6 in the list of references). In addition, it is reported that the extract of safflower has activity in inhibiting elastase (see patent 7 in the list of references). Additionally, the extract of safflower can be added to the anti-aging external preparation for the skin or the like, to activate cells (see patent 8 in the list of references).

List of literature related to the topic

[patent 2 in the reference list]

The Japan Patent No. 3748941

[patent 3 in the reference list]

International patent publication No. WO/2004/075621

[patent publication 4 in the reference list]

Japanese patent publication No. 6(1994)-23088

[patent 5 in the reference list]

Japanese patent publication of unexamined application No. 8(1996)-163983

[patent publication 6 in the reference list]

The Japan Patent No. 3839438

[patent 7 in the reference list]

Japanese patent publication of unexamined application No. 2000-319189

[patent publication 8 in the reference list]

The Japan Patent No. 3641699

The main purpose of the present invention is to develop tools for the growth of fibroblasts, which can increase the growth not only of young fibroblasts, but also the aging of fibroblasts and to be safe and effective.

Description of the invention

It was found that, in the case of using a combination of yeast extract and extract of safflower, cell growth significantly accelerated, even in the case of senescent fibroblasts. The present invention, the core is Vano on the above observations. For example, in accordance with the above-cited patent publication 8 from the list of literature and yeast extract, and an extract of safflower were previously known as means of activating cells. However, not previously been reported about the effect on the fibroblasts, the proliferative potential of cells, which decreases with age. Unexpectedly, it was found that when both the above-described component used in combination, achieve a synergistic increase in growth senescent fibroblasts.

The present invention relates to a tool for the growth of fibroblasts containing yeast extract and extract of safflower as an effective component.

The present invention also relates to a method for growth of fibroblasts, according to which use yeast extract and extract of safflower.

The present invention also relates to the anti-aging agent for the skin, containing yeast extract and extract of safflower as an effective component.

In those cases, when the yeast extract or the extract of safflower is used separately, it is not possible to achieve sufficient growth-stimulating effect on the aging of fibroblasts. However, using the two above-described components in combination, get a pronounced effect on the stimulation of cell growth, in relation to trusim fibroblasts, as in the case of young fibroblasts. In this connection, it is possible to effectively counteract aging.

The term "aging fibroblasts" in the context of the present description means the fibroblasts, which are characterized by a reduced number of functions, such as potential cell proliferation and the ability to produce extracellular matrix, either with age or as a result of the adverse effects of ultraviolet radiation, the active species of oxygen, etc.

As yeast extract should preferably yeast extract, derived from yeast, which were cultured in the culture medium containing glycosaminoglycan, and where indicated, the medium was then subjected to ultraviolet radiation and/or has been treated with hydrogen peroxide. It was found that when yeast was cultured in a nutrient culture medium containing glycosaminoglycan, under conditions of stress induced by ultraviolet radiation or hydrogen peroxide, they form a component that protects cells. Thus, through the use of yeast extract, obtained from the yeast culture, according to the above description, it is possible to more effectively strengthen the growth of fibroblasts.

The present invention also relates to a drug is mportant drug for skin containing means for fibroblast growth according to the present description. For example, in cases where the means for fibroblast growth according to the present invention is mixed with an external preparation for skin, such as a cosmetic product, it is possible to prevent or reduce the decline of functions and the number of fibroblasts in the connective tissue with age, etc. and effectively to prevent or reduce the symptoms of aging, such as wrinkles and sagging, as well as reducing skin softness and elasticity.

According to the present invention, when using a combination of yeast extract and extract of safflower, it is possible to achieve a pronounced effect on the stimulation of cell growth, in relation not only to young fibroblasts, but also to aging fibroblasts. In addition, the fibroblasts in the dermis Mature skin multiply, so that safely and effectively increases the softness and elasticity of the skin and no observed adverse effects on the skin. In this regard, due to the use of funds for fibroblast growth according to the present invention or the external preparation for skin which contains the means for the growth of fibroblasts, according to the present invention, it is possible to effectively prevent or reduce the symptoms of aging, such as wrinkles and sagging, as well as reducing soft the human skin and its elasticity.

Brief description of drawings

In Fig. 1 is a graph illustrating the effect of the combination of the extract of safflower and yeast extract on the growth of young or senescent fibroblasts.

The best way of carrying out the invention

The means for the growth of fibroblasts or the like according to the present invention contains yeast extract and extract of safflower as an effective component.

As yeast extract possible to use yeast extract obtained by extraction with a polar solvent; a decomposition product of a yeast obtained as a result of bacteriolysis yeast in the process of camerasmobile, acidolysis, enzymatic hydrolysis or the like, and which has been filtered; or an extract obtained by processing, in accordance with which the above decomposition product of the yeast is dried and then extracted with a polar solvent or the like

As fungi to obtain yeast extract, it is preferable to use the Ascomycetes yeast, for example, yeast of the genus, belonging to the family Endomycetaceae, such as the genus Eremascus or genus Endomyces; yeast of the genus, belonging to the family Saccharomycetaceae, such as the genus Schizosaccharomyces, the genus Nadsonia, genus Saccharomycodes, genus Hanseniaspora, genus Wickerhamia, the genus Saccharomyces, the genus Kluyveromyces, the genus Lodderomyces, genus Wingea, genus Endomycopsis, genus Pichia, the genus Hansenula, the genus Pachysolen, genus Citeromyces, the genus Debaryomyces, the genus Schwanniomyces, genus Dekker, the genus Saccharomycopsis or genus Lipomyces; or genus, belonging to the family Spermophthoraceae, such as the genus Spermophthora, genus Eremothecium, genus Crebrothecium, genus Ashbya, genus Nematospora, genus Metschnikowia or genus Coccidiascus. In particular, from the viewpoint of availability and the like, yeast extract preferably should be made of fungi, such as yeasts of the genus Saccharomyces, for example, brewer's yeast, sake yeast or Baker's yeast. According to the present invention, it is possible to use yeast extract, available in the commercial version under the trade name "yeast extract".

As the extracting solvent, it is possible to use water; saline; phosphate buffer; phosphate buffered saline; polar organic solvent, e.g. a lower alcohol such as methanol, ethanol, propanol or isopropanol; polyhydric alcohol such as 1,3-butyleneglycol, propylene glycol, dipropyleneglycol or glycerol; a simple ether, such as diethyl ether or DIPROPYLENE ether; esters such as ethyl acetate or butyl acetate; or a ketone such as acetone or ethylmethylketone. Use one kind of solvent or at least two kinds of solvents selected from the above solvents. In cases where the extraction is carried out with a solvent, yeast or decomposition products of the yeast can be subjected to lyophile the promotion and/or spray-dried and then proektirovanii. In addition, you may be held homogeneity in the extracting solvent or can also be produced by ultrasonic treatment. The temperature of extraction is lower and it ranges from about 0 ° C to a temperature equal to the boiling point of the solvent. The extraction time also varies depending on the form of the extracting solvent and temperature of extraction and typically ranges from about one hour to about five days.

According to the present invention as yeast extract is preferable to use yeast extract, derived from yeast, which is cultivated in a nutrient culture medium containing glycosaminoglycan, and which is then subjected to treatment with ultraviolet irradiation and/or treatment with hydrogen peroxide. It was shown that in those cases where the yeast is cultivated in a situation of stress caused by UV irradiation and/or hydrogen peroxide, cells produce protective component, protecting them from stress. In addition, in such cases, by adding nutrients peptone, fractions or the like to the culture medium increases the severity of the response to stress. A method of obtaining a yeast extract or the like described, for example, in U.S. patent No. 6461857. For example, yeast extract can be obtained in the framework of the way, where is availa able scientific C with which the yeast Saccharomyces cerevisiae, representing one of the types of Baker's yeast, cultured in a nutrient culture medium to which is added glycosaminoglycan taken from a source other than the animal, where the specified stress in cultured yeasts create by adding at sublethal proportions of hydrogen peroxide, i.e. in the amount of about 0.1 wt.% up to 2 wt.% from the whole mass of cultured yeast, and not necessarily due to the irradiation of yeast sublethal dose of ultraviolet radiation (e.g., UVA/UVB irradiation intensity of 31.5 MJ/cm2and where the cultivated yeast obtained by solubilization by camerasmobile, acidolysis or the like and then subjected to drying, water extraction, filtration, etc. in Addition, yeast extract, derived from yeast, which were cultured in culture medium containing glycosaminoglycan obtained from a source other than animal, and then were irradiated with UV light and treated with hydrogen peroxide, available in the commercial version under the trade name "Biodyne EMPP (trade mark)" from Arch Personal Care Products L. P.

Extract of safflower can be obtained by carrying out the extraction of safflower (Carthamus tinctorius L.) solvent. It is possible to use each of the parts of safflower plants, such as flower, leaf, stem, root p is running and root, a fully plant of safflower. It is preferable to use the flowers of the safflower, which are known as crude drugs "Kouka (Carthami Flos.)".

Safflower can be extracted by performing the operation in the raw state. However, from the point of view of efficiency of extraction, the extraction should be carried out preferably after processing, such as cutting, drying or spraying. The extraction is carried out by immersion in the extracting solvent. To improve the efficiency of extraction, conduct stirring or extracting solvent can be conducted homogenization. The temperature of extraction is usually reduced to values constituting from about 5 º C to the boiling point of the solvent. In this case, the extraction time is usually from about four hours to about 14 days.

As the extracting solvent can be used water; saline; phosphate buffer; phosphate buffered saline; polar organic solvent, e.g. a lower alcohol such as methanol, ethanol, propanol, isopropanol or butanol; polyhydric alcohol such as 1,3-butyleneglycol, propylene glycol, dipropyleneglycol, 1,2-pentanediol or glycerol; a simple ether such as ethyl ether, or propyl ether; esters, such as tracecut or butyl acetate; or ketone, such as acetone or ethylmethylketone, or a mixture of solvents containing at least two kinds of solvents selected from the above solvents.

For example, according to the present invention, it is preferable to use the extract of safflower, obtained by processing, in accordance with which the flowers of safflower extracted with 50% 1,3-butyleneglycol and then filtered. In addition, according to the present invention, can be used extract of safflower available in the commercial version.

As a means for the growth of fibroblasts or the like, according to the present invention, yeast extract and extract of safflower can be used in direct form. Alternatively, the means for the growth of fibroblasts or the like, according to the present invention can be used in any form such as solution, suspension, emulsion, cream, ointment, gel or powder preparation, making them appropriate additive such as a carrier, diluent, stabilizer, buffer, means for adjusting the pH or solvent. In addition, the means for the growth of fibroblasts or the like, according to the present invention may be encapsulated in a liposome, microcapsule or the like, in addition to the above prerequisites, the means for the growth of fibroblasts or other, who according to the present invention, can be made in the drug together with stabilizer and a means of enhancing the absorption, such as an antioxidant, an antiseptic or an agent absorbing ultraviolet rays, so that they do not interfere with the effects according to the present invention.

Means for fibroblast growth or the like, according to the present invention may be incorporated into the framework for external drug or the like, and further can be made external preparation for application to the skin.

Assuming that effects are achieved according to the present invention do not require any restrictions on the mixing ratios of components: yeast extract and extract of safflower in the composition of the external preparation according to the present invention. However, the proportion made yeast extract relative to the total weight of the external drug should preferably be from 0.00001 wt.% up to 10 wt.%, should preferably be from 0.001 wt.% up to 5 wt.% and should preferably range from about 0.005 wt.% up to 1 wt.%. The share made for mixing the extract of safflower relative to the total weight of the external drug should preferably be from 0.00001 wt.% up to 10 wt.%, should preferably be from 0.001 wt.% up to 5 wt.% and should preferably be from 0.001 wt.% up to 1 wt.%.

The preparation for external application to the skin is according to the present invention may include cosmetic preparations, pharmaceutical products, medical cosmetics, etc. and they preferably may be made in the composition of cosmetic products. Additionally, the preparation for external application to the skin according to the present invention can be presented in any form, such as a product type, an aqueous solution, the drug solubilizing type, the preparation of emulsion type, drug type oil liquid, the drug gel type, drug type, paste, drug type, ointments, aerosol drug type, drug double-layer type, including water-oil, the preparation of three-layer type, including water-oil-powder. Additionally, the preparation for external application to the skin according to the present invention can be presented in the form printed on the material of the carrier-fundamentals of flat shapes.

Additionally, the preparation for external application to the skin according to the present invention can be represented as the product of arbitrary shape and can be used for application in a variety of ways, subject to achieve the effect according to the present invention. For example, the preparation for external application to the skin according to the present invention can be made in the composition of the main cosmetic products for skin care, such as medication to cleanse the skin, cosmetically the lotion, cosmetic basis, which have the form of lotion or cream, lotion type of milk, cream, gel, serum (cosmetic liquid), or the mask; a cosmetic product for makeup application, such as the tone for the face; cosmetic sunscreen drug actions, such as sunscreen lotion or cream; cosmetic product for the body, such as a lotion or hand cream, lotion or foot cream or lotion or body cream; or hygienic cosmetics such as body soap or soap.

If necessary, in addition to the above prerequisites, preparation for external application to the skin according to the present invention may contain other arbitrary component, which is usually used in external preparations for skin, such as cosmetics and pharmaceuticals, and which can be obtained by the known procedure for this, the required form of the drug. For example, if necessary, the preparation for external application to the skin according to the present invention may contain a bleaching agent, a humectant, an antioxidant, an oil component, a means of absorbing ultraviolet radiation, surface-active agent, a thickener, alcohol, powder component, a dye, a water component or tool for p is Tania skin. In addition, it is possible to enter in the composition of the appropriate tool, amplifying secretion, such as the disodium salt editovat acid, trinacria salt editovat acid, sodium citrate, sodium polyphosphate, metaphosphate sodium or gluconic acid; drug such as caffeine, tannin, verapamil, tranexamic acid or its derivative, extract of licorice, glabridin, hot aqueous extract of the fruits of narrow-leaved pyracantha (Pyracantha angustifolia Schneid.), the crude drug, tocopherylacetate, glycyrrhizin acid or its derivative or its salt; a whitening agent, such as vitamin C, magnesium ascorbyl phosphate, ascorbicacid, arbutin or kojeve acid; or a saccharide, such as glucose, fructose, mannose, sucrose or trehalose.

Examples

Below the present invention is illustrated in non-limiting examples. In the examples below, Biodyne EMPP (trade mark) (supplied by Arch Personal Care Products L.P.) is used as yeast extract, and the extract of safflower is used Pharcolex In Safflower (supplied by the company ICHIMARUPHARCOS Co., Ltd.).

The study of the effect on growth of the fibroblasts

Using fibroblasts derived from the frontal region of a newborn child, which are considered as "young fibroblasts, and fibroblasts derived from the dermis of the skin this is a decade of women, which are considered as "senescent fibroblasts".

Fibroblasts, as in the case of young and senescent fibroblasts, cultured in culture medium DMEM containing 10% FTS, within 24 hours. After that, the culture medium is replaced with another culture medium containing 0.5% of FTS, which added 0.1% extract of safflower, the culture medium containing 0.5% of FTS, which was added 0.5% of yeast extract or culture medium containing 0.5% of FTS, which added 0.5% extract of safflower and 0.01% yeast extract, and further fibroblasts cultured for another 48 hours. Additionally, as a control option, similarly spend processing culture using a culture medium DMEM containing 0.5% FTS, which does not add the test substance. Upon completion of the processing of culture, appreciate the respiratory activity of fibroblasts using reagent alumaloy blue, and the indicator is used as a measure for assessing the growth of fibroblasts. Relative growth of fibroblasts (%) is calculated relative to the respiratory activity of the control which is taken for 100%.

The obtained results are illustrated in Fig. 1. Using only the extract of safflower, as in the case of young fibroblasts and senescent fibroblasts, fibroblast growth increases NESN is significant, and the effect on enhancing fibroblast growth considered to be low. The same is observed when using a single yeast extract, where the growth of young fibroblasts increases significantly, but the effect on the growth of fibroblasts, in relation to aging fibroblasts, was also low. However, adding a combination of an extract of safflower and yeast extract effect on accelerating the growth of fibroblasts, in particular with regard to aging fibroblasts is enhanced synergistically, and growth senescent fibroblasts increases to a level equivalent to the level of growth of young fibroblasts.

It has been suggested that, when using the combination of the extract of safflower and yeast extract, the increase in senescent fibroblasts, the proliferative potential of cells, which decreases with age, largely increase, and the growth of fibroblasts is enhanced even in the case of aging skin, and symptoms of skin aging, such as wrinkles and reduce its softness and elasticity, preventing or effectively corrected.

The following are representative examples of the external preparation for skin containing a means for fibroblast growth according to the present invention. The proportions of the blend components are given in terms of percentage of weight relative to the weight in the her tracks.

Example 1 (cream type water/oil)(wt.%)
(1) Dimethylpolysiloxane3,0
(2) Decamethylcyclopentasiloxane13,0
(3) Dodecamethylcyclohexasiloxane5,0
(4) a Copolymer of polyoxyethylene-methylpolysiloxanes1,0
(5) Pullulan 3-Tris(trimethylsiloxy)cellproperties acid1,0

(11) Ethanol
(6) Decamethylcyclopentasiloxane mixture stitched polymethylsiloxane (Dow Corning 9040 Silicone Elastomer Blend TM, supplied by Dow Corning Co., Ltd.)5,0
(7) Ricinoleate0,1
(8) an ester of para-hydroxybenzoic acidThe appropriate number
(9) L-mentholThe appropriate number
(10) Trimethylcyclohexylamine acid2,0
2,0
(12) Glycerin3,0
(13) Dipropyleneglycol5,0
(14) polyethylene Glycol 60005,0
(15) sodium Hexametaphosphate0,05
(16) tocopherol acetate0,1
(17) Caffeine0,1
(18) the Extract of fennel0,1
(19) witch hazel Extract0,1
(20) the Extract of safflower1,0
(21) Yeast extract1,0
(22) Trinacria salt editovat acid0,05
(23) Disorganization0,01
(24) Methylbis(trimethylsiloxy)similiter-trimetoksi-cinnamic acid0,1
(25) Inorganic composite particles (Cover Leaf AR-80TM, supply companies JGC Catalysts and Chemicals Ltd.) 5,0
(26) Yellow iron oxideThe appropriate number
(27) cobalt TitanateThe appropriate number
(28) Hectorite of dimethyldodecylamine1,5

(29) Polyvinyl alcohol : 0,1
(30) Hydroxyethylcellulose0,1
(31) a Copolymer of sodium acrylate/2-acrylamide-2-methylpropane-sulfonic acid (SIMULGEL EGTM supplied by the company SEPPIC Co., Ltd.)0,1
(32) Copolymer (acryloyldimethyltaurate/VP) (Aristoflex AVCTM supplied by Clariant Co., Ltd.)0,1
(33) Perfume additiveThe appropriate number
(34) Deionized waterThe residual quantity
Example 2 (milky body lotion)(wt.%)
Vaseline5
Beganovic alcohol0,5
Batrawy alcohol0,5
Glycerin7
1,3-butyleneglycol7
1,2-pentanediol1
Xylitol3
The polyethylene glycol 200002
Hydrogenated oil2
Jojoba oil2
Squalane5
Ezoterikova acid0,5
Pentaerythritol-Tetra-2-ethylhexanoate acid2
Polyoxyethylene-hydrogenated castor oil0,5
Lauryldimethylamine acid betaine0,4
The potassium hydroxideThe appropriate number
Sodium pyrosulphite 0,01

The sodium hexametaphosphate0,05
The glycyrrhizinate of dItalia0,05
Trimethylglycine3
Arbutin3
Yeast extract0,1
Tocopherol acetate0,1
Thiotaurine0,1
Extract of safflower1
Extract of Sophora flavescens0,1
Red iron oxideThe appropriate number
The seed extract of quince0,1
Carboxyvinyl polymer0,2
PhenoxyethanolThe appropriate number
Deionized waterThe residual quantity
Example 3 (milk lotion)(wt.%)
Dimethylpolysiloxane3
Decamethylcyclopentasiloxane4
Ethanol5
Glycerin6
1,3-butyleneglycol5
Polyoxyethyleneglycol3
Sunflower oil1
Squalane2
The potassium hydroxide0,1
The sodium hexametaphosphate0,05
Hydroxypropyl-β-cyclodextrin0,1
The glycyrrhizinate of dItalia0,05
The leaf extract of loquat Japanese0,1
MSG monolatry0,05
Extract of fennel 0,1

Yeast extract0,1
Lavender0,1
Extract of safflower0,1
The extract of the roots of Rehmannia Chinensis0,1
Disorganization0,1
Xanthan gum0,1
Carboxyvinyl polymer0,1
Copolymer of acrylic acid/alkylmethacrylamide (PEMULEN TR-1)0,1
Red iron oxideThe appropriate number
Yellow iron oxideThe appropriate number
ParabenThe appropriate number
Deionized waterThe residual quantity

The external preparation for skin according to each of the above examples, when applying it to age the skin, demonstrates excellent is th effect on reducing the severity of wrinkles.

1. Means for fibroblast growth containing from 0.01 to 1.0 wt.% yeast extract and 0.1 to 1.0 wt.% extract of the flowers of safflower as an effective component, in which yeast extract is a yeast extract obtained from yeast, which is grown in a nutrient culture medium containing glycosaminoglycan, and which is subjected to UV irradiation and treatment with hydrogen peroxide.

2. Means for fibroblast growth according to claim 1, where these fibroblasts are senescent fibroblasts.

3. The external preparation for skin containing a means for fibroblast growth according to claim 1 or 2.

4. The external preparation for skin according to claim 3, which is a cosmetic product.

5. The method of growing fibroblasts, which are used from 0.01 to 1.0 wt.% yeast extract and 0.1 to 1.0 wt.% extract of the flowers of the safflower, wherein said yeast extract is a yeast extract obtained from yeast, which is grown in a nutrient culture medium containing glycosaminoglycan, and which is subjected to UV irradiation and treatment with hydrogen peroxide.

6. The method according to claim 5, in which these fibroblasts are senescent fibroblasts.

7. Anti-aging agent for the skin, containing from 0.01 to 1.0 wt.% yeast e is stricta and from 0.1 to 1.0 wt.% extract of the flowers of safflower as an effective component, wherein said yeast extract is a yeast extract obtained from yeast, which is grown in a nutrient culture medium containing glycosaminoglycan, and which is subjected to UV irradiation and treatment with hydrogen peroxide.



 

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EFFECT: using the given polyurethanes in the sun-protection products enables their good water resistance, easy applicability, using comfort, no rolls, adhesiveness and greasy feeling.

2 cl, 5 ex, 5 tbl

FIELD: medicine.

SUBSTANCE: invention refers to a composition with the property of hair growth-stimulating effect. The declared composition contains at least one aromatase inhibitor specified in a group of chemically synthetised aromatase inhibitors, and soya bean and rape extracts, as well as at least one herbal extract. The above extract contains one or more biologically active substances extracted from plants and specified in a group consisting of type I and/or type II 5α-reductase inhibitors and androgen receptor blockers. Also, the invention refers to using the declared composition for cosmetic application and body or face hair growth reduction and/or scalp hair growth stimulation.

EFFECT: invention provides the controlled hair growth and pigmentation effect.

15 cl, 14 ex

FIELD: medicine.

SUBSTANCE: invention refers to a composition with the property of hair growth-stimulating effect. The declared composition contains at least one aromatase inhibitor specified in a group of chemically synthetised aromatase inhibitors, and soya bean and rape extracts, as well as at least one herbal extract. The above extract contains one or more biologically active substances extracted from plants and specified in a group consisting of type I and/or type II 5α-reductase inhibitors and androgen receptor blockers. Also, the invention refers to using the declared composition for cosmetic application and body or face hair growth reduction and/or scalp hair growth stimulation.

EFFECT: invention provides the controlled hair growth and pigmentation effect.

15 cl, 14 ex

FIELD: medicine.

SUBSTANCE: invention refers to cosmetology and represents a cosmetic composition for making hair care products, containing amino acids, proteins, saccharides, vitamins, functional additives and water, differing by the fact that it additionally contains shark liver oil with the ingredients of the composition taken in certain proportions, wt %.

EFFECT: invention provides reducing hair loss considerably.

3 ex

FIELD: chemistry.

SUBSTANCE: invention relates to vegetable dyes obtained from the coloured part of plant types, the cell tissue of which contains iridisomes that are responsible for colouring of said part. In particular, the invention relates to a blue dye obtained from seed skin of plant type Ravenala madagascariensis. The invention also relate to compositions containing said dye, particularly cosmetic compositions, especially meant for paining skin or external formations on the body.

EFFECT: invention provides cheap and safe production of a natural dye with a bright colour with a possible interference effect from a vegetable ultrastructure without denaturing thereof.

20 cl, 4 ex

FIELD: medicine.

SUBSTANCE: invention relates to method of obtaining medication for roentgenologic examination by processing suspension of tantalate of element, selected from group, which includes yttrium, lanthanum, cerium, praseodymium, neodymium, samarium, europium, gadolinium, terbium, dysprosium, holmium, erbium, thulium, ytterbium, lutetium in mode of percussive mechanical loadings with intensity not less than 10 g, in presence of sodium salt of carboxymethylcellulose (Na-CMC), taken in quantity 0.5-1.5 wt % from the total mass.

EFFECT: invention ensures high contrast of image and stability of medication for a long time due to obtaining suspension, which contains nano-size particles.

2 dwg, 3 ex

FIELD: medicine.

SUBSTANCE: invention relates to selective method of introducing fluorine atom into biomolecule. Method includes supply of linker, which contains thiol-reactive end and azido- or alkine-reactive end, interaction of thiol-reactive end of linker with biomolecule, which contains at least one thiol group or thiol group derivative, which can be activated in order to form free thiol group and the following interaction of azido- or alkine-reactive end of linker with fluorine-substituted azide or alkine group, where linker represents: or . Method makes it possible to selectively bind linker to biomolecule and selectively bind fluorine at final stage, which eliminates necessity of additional purification stages.

EFFECT: invention also relates to linkers claimed in method.

6 cl, 5 dwg, 1 tbl, 9 ex

FIELD: medicine, pharmaceutics.

SUBSTANCE: invention relates to pharmaceutical industry and deals with creation of medication of vegetable origin based on biologically active substances of Linaria vulgaris, which has diuretic and anticoagulation activity. Method of obtaining medication, which has diuretic and anticoagulation activity, consists in the following: extraction of milled grass of Linaria vulgaris with ethyl alcohol is carried out with successive drawing with 3 parts of extractant by method of remaceration with heating and connecting vacuum at the stage of discharge, under specified conditions.

EFFECT: method makes it possible to obtain medication, which has high diuretic and anticoagulation activity.

3 tbl, 1 ex

FIELD: medicine, pharmaceutics.

SUBSTANCE: invention relates to pharmaceutical industry, namely to biologically active compound, which has analgesic action. Lignan, which has analgesic action, whose structure is described as (1R,2S)-1-[(1R,2S)-1-(3,4-dihydroxyphenyl)-6,7-dihydroxy-3 - [(1R,2S)-1,2,3-tricarboxypropoxy]carbonyl-1,2-dihydronaphthalene-2-carbonyl]oxypropane-1,2,3-tricarboxylic acid: .

EFFECT: lignan has directed action at proton-driven ion channel ASIC3, which initiates neuronal response under pain stimuli.

7 dwg, 8 ex

FIELD: medicine, pharmaceutics.

SUBSTANCE: invention relates to pharmaceutical industry, in particular to method of obtaining immunotropic preparation. Method of obtaining immunotropic preparation by extraction of air-dry leaves of Lophanthus anisatus Benth with aqueous ethyl alcohol, heating, cooling, filtering, solvent evaporation, residue dissolving and extraction with chloroform, filtering and drying of sediment, under specified conditions.

EFFECT: method makes it possible to obtain efficient immunotropic medication.

3 tbl, 5 ex

FIELD: medicine, pharmaceutics.

SUBSTANCE: invention relates to pharmaceutical industry, and namely to composition for prevention of iron deficiency anemia. Composition for prevention of iron deficiency anemia, which contains source of iron, source of copper, vitamins B1, B3, B6, B9, B12, ascorbic acid, extract from nettle leaves, extract from strawberry leaves, pantohematogen and auxiliary substances, with specified component ratio.

EFFECT: composition makes it possible to extend arsenal of methods for prevention of iron deficiency anemia and is effective.

4 cl, 3 ex

FIELD: medicine, pharmaceutics.

SUBSTANCE: invention relates to pharmaceutical industry, and namely to composition for prevention of iron deficiency anemia. Composition for prevention of iron deficiency anemia, which contains source of iron, source of copper, vitamins B1, B3, B6, B9, B12, ascorbic acid, extract from nettle leaves, extract from strawberry leaves, pantohematogen and auxiliary substances, with specified component ratio.

EFFECT: composition makes it possible to extend arsenal of methods for prevention of iron deficiency anemia and is effective.

4 cl, 3 ex

FIELD: medicine.

SUBSTANCE: invention refers to cosmetic and pharmaceutical industry, particularly to a method for preparing a water-in-oil extract of herbs and/or flowers. The method for preparing a water-in-oil extract of herbs and/or flowers involving pre-watering or moistening of the herbal raw material in deionised water, and further extraction of the wet or swollen raw material by single-flow percolation or static mixing with an extractant presented by a water-in-oil emulsion containing oil phase 3-15 wt % specified in a group consisting of natural milk, curdy whey or a mixture thereof or a mixture thereof with milk whey under certain conditions.

EFFECT: method enables preparing the extract with the high content of biologically active substances while being highly effective and ecologically-friendly.

5 cl, 10 ex

Wound healing agent // 2491945

FIELD: medicine, pharmaceutics.

SUBSTANCE: invention refers to pharmaceutical industry and medicine, and represents a wound healing agent containing a concentrated raw material from antler-producing deers, differing by the fact that the raw material represents a concentrate of deer's velvet antlers and a concentrate of mixed wild chamomile, birch buds and common clotbur taken in ratio 1:4:5 respectively, extracted and dried in ratio of the mixture of herbal ingredients: water 1:20, with the agent additionally containing stearic acid, glycerol monostearate, bee wax, cosmetic wax, lanolin, vegetable oil, glycerol, sodium tetraborate, triethanolamine, phenochem, polyethylsiloxane liquid, cyclomethicone, an antifoam additive, lidocaine + a flavour, water, with the ingredients of the agent taken in certain proportions, wt %.

EFFECT: invention provides high wound healing action, and long shelf life.

2 cl, 3 tbl

FIELD: chemistry.

SUBSTANCE: invention relates to vegetable dyes obtained from the coloured part of plant types, the cell tissue of which contains iridisomes that are responsible for colouring of said part. In particular, the invention relates to a blue dye obtained from seed skin of plant type Ravenala madagascariensis. The invention also relate to compositions containing said dye, particularly cosmetic compositions, especially meant for paining skin or external formations on the body.

EFFECT: invention provides cheap and safe production of a natural dye with a bright colour with a possible interference effect from a vegetable ultrastructure without denaturing thereof.

20 cl, 4 ex

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