Method for prediction of clinical effectiveness of diabetic polyneuropathy in patients with type 2 diabetes mellitus and dyslipidemia
SUBSTANCE: invention refers to medicine, namely endocrinology, and concerns predicting the clinical effectiveness in diabetic polyneuropathy in the patients suffering type 2 diabetes and dyslipidemia. That is ensured by evaluating the intensity of diabetic polyneuropathy manifestations taking into account neuropathic dysfunctional score, evaluating triglycerides and glycolised blood hemoglobin before the treatment; the derived data are used to specify a therapeutic approach followed by further estimation of a probability of a successful correction of diabetic polyneuropathy by formula
EFFECT: method provides the improved clinical effectiveness ensured by the individual therapeutic approach based on the lipid and carbohydrate metabolism of a specific patient.
The invention relates to medicine, namely to endocrinology and diabetology and can be used to predict the effectiveness of treatment of diabetic polyneuropathy according to a study of source parameters of carbohydrate and lipid metabolism, the severity of polyneuropathy and application of different schemes hypoglycemic and hypolipidemic drugs.
Diabetic polyneuropathy (SFD) is one of the most frequent and serious complications of diabetes mellitus (DM). The duration of type 2 diabetes for more than 25 years of cash flow detected in more than 50% of patients (Kempler P. Diabetic neuropathy: clinical problems and possible approaches to treatment. // Complications of diabetes mellitus: pathophysiology and pathogenetic treatment options. Edited PG. Cornelli and Canlera. International working meeting of experts. Rome (Italy), 2008 - P.36-45). Recent evidence that the cash flow occurs in 13% of cases among patients with impaired glucose tolerance and 11.3% - elevated levels of fasting plasma glucose (Ziegler D, Rathmann W, et al. Prevalence of Polyneuropathy in Prediabetes and Diabetes is Associated with Abdominal Obesity and Macroangiopathy. The MONICA/KORA Augsburg Surveys S2 and S3 // Diabetes Care. - 2008. - Vol.31. - P.464-469) suggests that cash flow is not only one of the most common complications of type 2 diabetes, but may develop much earlier clinical manifestation of this disease. In this connection, only carbohydrate metabolism cannot explain how the development and progression of cash flow. In 2009, the study Wiggin T.D. et al. a correlation was found elevated levels of triglycerides with the progression of diabetic polyneuropathy (Wiggin T.D., Sullivan K.A, Pop-Busui R., A. Amato, A.A. Sima, E.L. Feldman Elevated Triglycerides Correlate with Progression of Diabetic Neuropathy. // Diabetes published online. May 1. - 2009. - as db08-1771). According to the authors, the correlation between triglycerides and progression of diabetic neuropathy suggests that hyperglycemia and impaired glucose metabolism are not the only factors contributing to nerve damage, it is necessary to take into account the nature of lipid metabolism.
There is a method of treatment of diabetic polyneuropathy (RF patent No. 2191011, publ. 20.10.2002), namely, that after reaching compensation of diabetes mellitus with insulin and/or glucose-lowering drugs for patients additionally prescribe thyroid hormones in the dose of from 12.5 to 50 μg. The use of thyroid hormones in the treatment method cash flow leads to an increase in the velocity of propagation of excitation in n. tibialis, n. peroneus, n. suralis determined by electroneuromyography, and hence to improvements in vibratory, tactile, temperature sensitivity as diabetic patients with thyroid disease, and the patient is in DM in the absence of thyroid disease. Presumably, this is achieved by activation of antioxidant protection, increase oxygen delivery to the periphery, reducing tissue hypoxia and improves the utilization of glucose, overcoming insulin resistance. The disadvantage of this method is that thyroid hormones are contentware problematic to use in the absence of a pathology of a thyroid gland, and this positive effect may be caused by weight loss and correction of atherogenic dyslipidemia. In this regard, pathogenetically more justified is the use of lipid-lowering drugs.
Also known is a method for predicting diabetic peripheral neuropathy in children and adolescents (RF patent No. 2273028, publ. 27.03.2006). The method includes defining in the peripheral blood serologic method of brain-derived neurotrophic factor. When the value of subjects neurotrophin more than 9000 PG/ml diagnose subclinical stage of cash flow. The method allows to predict the complication in diabetes mellitus type 1 in children and adolescents and early preventive treatment. However, the implementation of the method does not take into account characteristics of the underlying disease, the degree of metabolic changes in the patient, which reduces the objectivity of the prediction method, acetylaminophenol formation of cash flow.
The task of the invention is to provide a method for predicting the effectiveness of treatment of diabetic polyneuropathy with the help of various schemes combination of antidiabetic and gipolipidemicheskih drugs with the original parameters of carbohydrate and lipid metabolism, the severity of neuropathy and anamnestic data.
This task is achieved by the fact that patients define duration of T2DM (Dlcd) in years, appreciate the severity of the manifestations of diabetic polyneuropathy (Virden) on the scores of the questionnaire neuropathic dysfunctional account, determine the content of triglycerides (TG) and glycated hemoglobin (HbAlc) in the blood before treatment. Choose the treatment strategy (Ctrl), ranging as follows: 1 point - standard glucose-lowering therapy: Metformin + diabeton MB; 2 points - glucose-lowering therapy with a combination of drugs that reduce insulin resistance, and entretenimiento (Metformin + ecstatic); 3 points - glucose-lowering and lipid-lowering therapy (Metformin, ecstatic, statins); 4 points - glucose-lowering and lipid-lowering therapy, including the fibrates (Metformin, ecstatic, statins, fibrates). Determine the probability of successful correction of the cash flow formula
The novelty of the invention lies in the fact that simultaneous assessment of anamnestic data, parameters of carbohydrate and lipid metabolism, points on a scale VAT, the use of multiple regression analysis, which is the main in multivariate statistics allow to increase the sensitivity of the predictive determine the effectiveness of treatment are still at the planning stage of its use.
The invention involves an inventive step, because the endocrinologist is not obvious from the level of medicine in the field of diabetology.
In the available sources of Russia and foreign't found similar to the proposed method of predicting the effectiveness of treatment of cash flow.
The claimed invention is industrially applicable as there may be many times repeated and used on ence treatment effectiveness cash flow and reproduced in various medical and scientific medical institutions, especially endocrinology and therapeutic profile.
A method for predicting the effectiveness of treatment of diabetic polyneuropathy in patients with diabetes mellitus type 2 and dyslipidemia, as follows.
In a patient with type 2 diabetes clarify, when he was first diagnosed and determine the duration of disease in years - Dlsd.
On a scale of neuropathic dysfunctional account (VAT) specify in points the severity of peripheral polyneuropathie - Virden (Young M.J., Zhou Y.Q., E. Rodrigues et al. Variable relationship between peripheral somatic and autonomic neuropathy in patients with different syndromes of diabetic polyneuropathy // Diabetes. - 1986. - Vol.35. - P.192-197; Chernyshev, IE, I.V. Guryev, Altynbaev R.A. with Soave. Diabetic neuropathy (pathogenesis, diagnosis, treatment). - M.: publishing house MEDPRAKTIKA - M, 2006. - P.39-40), and rank scores for the scale of VAT in the following way: 1 point - the initial cash flow (the number of points in the questionnaire VAT initial cash flow (the number of points in the questionnaire VAT 0-4 points), 2 points - moderate cash flow (the number of points in the questionnaire VAT 5-13 points)3 points - severe cash flow (the number of points on the scale of VAT 14 points or more).
In whole blood of the patient determine the level of glycosylated hemoglobin in %, for example, using the fast ion-exchange method using reagents "HUMAN"firm, Germany (Ostapenko V.A., Firstova, L.P., Eliseeva I.P. with Soave. Optim is within the definition of a stable fraction of glycosylated hemoglobin HbAlc method of indexing the total fraction of glycosylated hemoglobin HbAl // Fundamental research. - 2008. No. 6 - P.116-117).
Serum determine the level of triglycerides in mmol/l, for example, by using a set of reagents "Cleanitest-Cholesterol", produced by SPC Eco-Service" (Kiskun A.A. guidelines on laboratory methods of diagnosis. - M.: GEOTAR - Media, 2007. - S).
Analyzing carbohydrate and lipid metabolism, determine the treatment regimen of the four below, ranging its relevant points: 1 point - standard glucose-lowering therapy: Metformin + diabeton MB; 2 points - glucose-lowering therapy with a combination of drugs that reduce insulin resistance, and entretenimiento (Metformin + ecstatic); 3 points - glucose-lowering and lipid-lowering therapy (Metformin, ecstatic, statins); 4 points - glucose-lowering and lipid-lowering therapy, including the fibrates (Metformin, ecstatic, statins, fibrates).
Further calculates the regression coefficient Z by the formula:
where Dlsd - duration type 2 diabetes, defined in years;
Virden - cash flow severity: 1 point - the initial cash flow (the number of points in the questionnaire VAT 0-4 points), 2 points - moderate cash flow (the number of points in the questionnaire VAT 5-13 points)3 points - severe cash flow (the number of points on the scale of VAT 14 points or more);
TG - triglycerides blood before treatment in mmol/l;
HbAlc - glycosylated is p hemoglobin before treatment in%;
Ctrl strategy proposed treatment: 1 point - standard glucose-lowering therapy: Metformin + diabeton MB; 2 points - glucose-lowering therapy with a combination of drugs that reduce insulin resistance, and entretenimiento (Metformin + ecstatic); 3 points - glucose-lowering and lipid-lowering therapy (Metformin, ecstatic, statins); 4 points - glucose-lowering and lipid-lowering therapy, including the fibrates (Metformin, ecstatic, statins, fibrates).
The calculated Z value substituted into the formula to determine the probability of successful correction cash flow in patients with type 2 diabetes and dyslipidemia (in percentage units), which has the following form:
where e is the base of natural logarithm, e=2,72;
Z - factor regression.
If the P value is 0.7 or more, the patient is predicted successful correction of the cash flow. When P has a value less than 0.7, it is necessary to change the treatment strategy and re-calculate R when a different number of points to select a more successful method of combination of drugs in individual sieve is tion.
The proposed method eliminates the presence of subjectivity. The method will allow the doctor to assess the possibility of successful adjustment cash flow, to predict its course, promptly appoint an adequate therapy. This will reduce the risk of late vascular complications of diabetes and improve the health and quality of life of patients.
The operability of the invention the following examples.
For example, the patient D., 58 years, type 2 diabetes was diagnosed at the age of 55, lasted 3 years, the questionnaire VAT number of points was 11, therefore, the severity of cash flow was moderate (2 points), before treatment triglycerides blood was 2.1 mmol/l) glycated hemoglobin was 7.1%. The patient was assigned to a combination of glucose-lowering and lipid-lowering therapy with Metformin, ecstatic, simvastatin, fenofibrate (4 points).
First calculate the Z-factor=4,56-0,05·3-0,02·2-0,05·2,1-0,53·7,1+0,14·4=1,04,
Then we substitute it into the formula
Therefore, the probability of successful correction cash flow in Tomlinson case is equal to 0.7 or 70%.
Another clinical example. Patient A., 64 years, experience of diabetes was 12 years and questionnaire VAT scores corresponded to 15, therefore cash flow was severe (3 points), the original content of triglycerides blood was 2.6 mmol/l) glycated hemoglobin was 7.4%. The patient was assigned only antidiabetic therapy Metformin and ecstatic (2 points).
First calculate the Z-factor=4,56-0,05·12-0,02·3-0,05·2,6-0,53·7,4+0,14·2=0,06
Then we substitute it into the formula
Therefore, the probability of successful correction of the cash flow that the patient had a low - to 0.29 or 29% and was determined not feasible to use this method of treatment.
Determination of the significance of all of the studied parameters for predicting the likelihood of effective treatment cash flow was high, because the determination coefficient was R2=0,82. Therefore, created a regression model in 82% was explained by the effectiveness of the treatment. The Fisher's F amounted to 27.3 (p<0,001), indicating a high statistical significance regr ssional model. The coefficient of multiple correlation, reflecting the relationship between the original variables and the probability of good results, had a value of 0.91, indicating their strong interaction. The coefficient of determination of residues (that is unaccounted for variables in the model) was a minor R2=0,12, testified that the risk of uncontrolled flow was due mainly it is accounted for in the regression model parameters and did not depend on factors not considered.
To check the quality of the model was performed ROC analysis with the construction of curves of sensitivity and specificity. ROC curve reflects the dependence of the number of correctly classified positive examples from the number of incorrectly classied negative examples. The calculated area under the ROC curve was 0.71, which corresponds to "good" as a model.
Technical and economic efficiency of the proposed method is that the method allows to choose an effective way to ensure cash flow and prevent the development of ulcers of the feet, diabetic foot syndrome, which reduces the risk of disability of the patient and they lose their social and labour potential.
A method for predicting the effectiveness of treatment of diabetic polyneuropathy (SFD) is diabetes mellitus (DM) type 2 and dyslipidemia,
namely, that patients with diabetes mellitus type 2 and dyslipidemia determine the duration of type 2 diabetes (Dlcd) in years, appreciate the severity of the manifestations of diabetic polyneuropathy (Virden) taking into account the points of neuropathic dysfunctional account (VAT): 1 point severity cash flow corresponds 0-4 scores on questionnaire VAT, 2 points severity of cash flow - 5-13 scores on questionnaire VAT, 3 credits severity of cash flow is 14 or more points on questionnaire VAT; determine the content of triglycerides (TG) and glycated hemoglobin (HbAlc) in the blood before treatment and choose the treatment strategy (Ctrl): 1 point - standard glucose-lowering therapy Metformin + diabeton MB; 2 points - glucose-lowering therapy with a combination of drugs that reduce insulin resistance and entretenimiento - Metformin + ecstatic; 3 points - glucose-lowering and lipid-lowering therapy Metformin + ecstatic + statins; 4 points-glucose-lowering and lipid-lowering therapy, including fibrates - Metformin + ecstatic + statins + fibrates; then determine the probability of successful correction cash flow in fractions of a unit by the formula
where e is the base of natural logarithm e=2,72; Z - regression coefficient, calculated according to the formula Z=4,56-0,05·Dlsd-0,02·Virden-0,05·TG-0,53·HbAlc+0,TL, if the P value is 0.7 or more, patients predict successful correction of cash flow, and if the P value has a value less than 0.7, it is necessary to change the treatment strategy.
SUBSTANCE: diagnosticum comprises human lymphocytes to identify donor-specific major histocompatibility complex antigen antibodies during the post-transplant period, characterised by the fact that the diagnosticum comprises lymphocytes obtained from the multiorgan donor's spleen; a method for preparing a diagnosticum, predicting a rejection reactions following the transplantation from multiorgan donors, including a reaction to identify the donor-specific major histocompatibility complex antigen antibodies, characterised by the fact that the above diagnosticum is used. The diagnosticum contains T lymphocytes bearing the class I major histocompatibility complex antigens, and B lymphocytes bearing the class II major histocompatibility complex antigens in ratio suitable to provide an adequate antigen-antibody reaction in studying the donor-specific class I and II HLA antibodies in recipient's serum for a long transplantation period for humoral rejection diagnosis and evaluation of the immunosuppression therapy effectiveness.
EFFECT: preparing the high-quality diagnosticum.
4 cl, 2 ex, 2 tbl
SUBSTANCE: method is implemented by detecting a marker in herparinised venous blood by incubating the blood with a mycobacterial antigen material (a test sample) and with normal saline (a reference); thereafter the blood is examined to measure CD45+ lymphocytes of the fluoroisothiocyanate-labelled markers; the fluorescence of the labelled CD45+ lymphocytes in the test sample in relation to the reference enables to consider that the individual has been infected with the very species of mycobacteria with the antigen material has been used for the blood incubation in a test tube.
EFFECT: method enables supporting for the fact of the mycobacterial infection, identifying the infection agent species, assessing the vaccination effectiveness; the method can be implemented for a relatively short time.
FIELD: medicine, pharmaceutics.
SUBSTANCE: invention discloses using the monoclonal antibodies (MCAs) produced by the hybridomas B/4H1 and 4H7 and directed to variable determinants as a part of influenza B virus proteins. The hybridoma B/4H1 and 4H7 strains are deposited in the Russian Collection of Vertebral Cell Cultures (RKKK P) under Nos. 719 D and 720 D, respectively. The two current influenza B viral evolution lines are identified with using the MCAs B/4H1 and MCAs 4H7 directed to variable determinants of Victorian-type and Yamagata-type influenza B hemagglutinin, respectively.
EFFECT: using the prepared MCA kit enables identifying the newly recovered influenza B viruses Besides, the MCAs under the invention may be used to study the antigenic structure and variability of this agent, to identify them as belonging to a specific evolution line; the MCAs may be also used to construct the diagnostic test systems.
3 cl, 12 dwg, 8 ex
FIELD: medicine, pharmaceutics.
SUBSTANCE: invention describes a chemically modified peptide that is a fragment of human protein S100β consisting of a sequence of 15 amino acids corresponding to the amino acids in position 18-32 of the amino acid sequence of protein S100β. The peptide is covalently bound to a spacer (Ahx) consisting of 2-aminoheptane acid or a similar compound, and covalently bound to the spacer by a biotin molecule (Bio). The peptide is described by general formula: A-Tyr1-Ser2-Gly3-Arg4-Glu5-Gly6-Asp7-Lys8-His9-Lys10-Leu11-Lys12-Lys13-Ser14-Glu15-E, wherein A represents Bio-Ahx, Bio-Acp, Bio or 0, and B represents 0 or -NH2. Bio means biotin; Ahx means a residue of 2-aminoheptane acid; Acp means a residue of 6-aminocapronic acid, 0 means the absence of any amino acid. A diagnostic technique and a method for prediction of melanoma provides measuring blood natural antibodies (nAB) to the peptide according to the invention with the stages of melanoma diagnosed by the antibody concentration as compared to the reference. The clinical outcome and therapeutic effectiveness in a craniocereberal injury are predicted by measuring the nAB to the peptide according to the invention. If observing the blood nAB level in the patients lower as compared to the standard reference, the favourable clinical outcome is diagnosed, and the therapy is stated to be effective.
EFFECT: invention may be used effectively as instant diagnosing of the disturbed blood-brain barrier and monitoring of the clinical effectiveness in CCIs and some cancers.
3 cl, 9 dwg, 1 tbl, 5 ex
SUBSTANCE: technique according to the invention involves the two-stage immobilisation of S and R brucellosis antigens on a tray by a non-specifically adsorbed mouse's monoclonal antibodies 2H2 and 2H8 in wells of the immunological tray (the first stage), specific binding S and R brucellosis antigens (the second stage) thereto, introduction and incubation of the analysed material (blood serum or milk) combined with introduction of mixed mouse's monoclonal antibodies 2H2 and 2H8 marked by horseradish peroxidase. The brucellosis agent antibodies are detected by being competitive with the antigen in binding on the well surfaces of the tray between the antibodies of the analysed sampled and fragment-marked monoclonal antibodies. A decreased level of the enzymatic signal in the analysed sample as compared with the negative reference testifies to contamination of the animal. Introducing the analysed sample into the two wells of the tray with S and R brucellosis antigens enables differentiation thereof for antibody specificity to any given form of the disease agent.
EFFECT: method involving the enzyme immunoassay enables increasing the effectiveness of health-improving measures, reducing the length of health-improvement in livestock farms with the negative brucellosis situation, dropping the disease incidence.
3 tbl, 3 ex
SUBSTANCE: ultrasound-assisted transcutaneous targeted needle liver-biopsy is used in the patients with acute destructive pancreatitis; that is followed by the immunohistological analysis of the biopsy micropreparations in parallel sections 5 mcm thick marked with monoclonal antibodies: CD 79a for identifying B-Lm and CD 68 for identifying MF. That is followed by optical microscopy at magnification x400 with morphometry of B-Lm periportal regions and MF lobe parenchyma; the results are further processed by automated image analysis systems. A relative area taken by the analysed cell elements in the micropreparation is determined by formula (A). If the value A for the B-Lm periportal regions falls within the range of 0.4 to 1.14, while the value A for the MF lobe parenchyma falls within the range of 0.89 to 2.95, a pre-infectious phase of acute destructive pancreatitis is stated. If the value A for the B-Lm periportal regions falls within the range of 1.41 to 4.98, while the value A for the MF lobe parenchyma falls within the range of 4.34 to 9.52, a phase of suppurative septic complications of acute destructive pancreatitis is stated.
EFFECT: using the method provides the well-timed correction of the therapeutic actions; the method is attended by a lower risk of the needle biopsy; it possess high accuracy and simplicity of implementation.
2 tbl, 2 ex, 6 dwg
SUBSTANCE: total pre-therapeutic immunoglobulin E is measured in blood serum of the patients suffering the aggravated chronic obstructive pulmonary diseases (COPD), and if observing the upper normal limits to be overridden, the high efficacy of the systemic glucocorticoid therapy is predicted.
EFFECT: using the declared method enables predicting the efficacy of the systemic glucocorticoid therapy.
2 tbl, 2 ex
SUBSTANCE: according to the method, the amniotic fluid substance P is determined at the beginning of the stage I of labour or antepartum amniorrhea using ELISA. If the substance P value is equal to 2.9 ng/ml and higher, dysthyroidism is predicted.
EFFECT: using the method enables higher accuracy of the preclinical dysthyroidism prediction and enables applying the well-timed obstetric approach.
SUBSTANCE: epidural electric stimulation of the spinal cord in the patients suffering a complicated superior cervical spine injury is preceded by preparing blood serum samples to be tested by enzyme immunoassay. A value of recombinant human ciliary neurotrophic factor CNTF is derived and accepted as a reference - Pref. Similarly, on the 7th day from the beginning of the epidural electric stimulation of the spinal cord, a value of CNTF - Pcur is determined and compared to the reference. If observing Pref.<Pcur., higher activation of the functional status of spinal motoneurons is stated.
EFFECT: invention provides more objective assessment combined with improved sensitivity and specificity of the declared method.
SUBSTANCE: present invention relates to immunology. Disclosed is an anti-α5β1 antibody, which is described through amino acid sequences of six hypervariable regions and an antigen-binding moiety thereof. Described are conjugates of the disclosed antibodies with a medicinal agent or a label, a pharmaceutical composition, use of the disclosed antibodies to prepare a medicinal agent, methods and an industrial product for inhibiting angiogenesis and/or vascular permeability in a subject, and for treating cancer, an ophthalmic disease and an autoimmune disease in a subject. The invention describes an isolated nucleic acid, an expression vector, a cell and a method of producing an antibody or an antigen-binding moiety thereof, as well as a method of detecting α5β1 protein in a sample.
EFFECT: present invention can find further use in therapy and diagnosis of α5β1-mediated diseases.
52 cl, 11 dwg, 6 ex
SUBSTANCE: system refers to endocrinology, and can be used for dry powder delivery to the airways. What is presented is an inhalation system comprising a dry powder inhaler and a dry powder. The system comprises a breath-actuated inhaler, or may be configured to release a powder jet. The inhalation system can change the air flow resistance value from approximately 0.065 to approximately 0.200 (kPa)/litre per minute. The powder contains a number of powdered diketopiperazine particles with a geometric mean diameter ranging within 2 mcm to 8 mcm and a geometric standard deviation less than 4 mcm. Powdered diketopiperazine administered per one inhalation and measured in plasma has AUC0-∞ more than 2300 ng×min/ml per mg in the dry powder. What is presented is the inhalation system configured to release more than 90% of the dry powder. After one inhalation, diketopiperazine particles are dissolved and absorbed in blood for less than 30 minutes with the peak concentration. The powder may contain a number of powdered insulin particles. Insulin administered per one inhalation and measured in exposed patient's plasma of AUC0-2h makes more than 160 mcUnits×min/ml per a unit of insulin.
EFFECT: invention provides creating the inhalation system with the uniform properties of powder delivery, easy to use and configure to allow the patient to comply with the treatment regimen better.
18 cl, 11 tbl, 35 dwg, 9 ex
FIELD: medicine, pharmaceutics.
SUBSTANCE: invention refers to a new lipid compound of general formula , wherein n=0; R1 and R2 are identical or different, and may be specified in a group of substitutes consisting of a hydrogen atom, a C1-C7alkyl group, a halogen atom and a C1-C7alkoxy group; X represents COR3 or CH2OR4, wherein R3 is specified in a group consisting of hydrogen, hydroxy, C1-C7alkoxy and amino; and R4 is specified in a group consisting of hydrogen, C1-C7alkyl or C1-C7acyl, Y represents C9-C21 alkene with one or more double bonds in E- or Z-configurations with the chain Y being unsubstituted and containing a double bond in the ω-3 position; provided R1 and R2 cannot simultaneously represent a hydrogen atom.
EFFECT: invention refers to pharmaceutical compositions containing the lipid compounds which are used for treating and/or preventing the conditions related to high NFkB functions, treating and/or preventing an inflammatory disease or a condition, lower plasma insulin and/or blood glucose levels, treating insulin resistance, treating and/or preventing peripheral tissue insulin resistance and/or diabetic condition, eg type 2 diabetes mellitus.
45 cl, 1 tbl, 1 dwg, 31 ex
SUBSTANCE: group of inventions refers to medicine, particularly a method of treating hyperglycemia and/or diabetes, reduction of glucose levels, as well as kits for treating. A method involves the meal-time rapid introduction of the GLP-1 dosage form into pulmonary circulation, e.g. by inhalation immediately into pulmonary alveolar capillaries with the use of a drug delivery system in the form of a dry powder wherein said therapeutically effective amount leads to the blood GLP-1 concentration which is 100 pmole/l or more.
EFFECT: method causes at least none side effects, such as excessive sweating, nausea and vomiting which are usually associated with the subcutaneous and intravenous introduction of glucagon-like peptide GLP-1.
25 cl, 8 ex, 6 tbl, 20 dwg
SUBSTANCE: invention relates to medicine, namely to endocrinology and cardiology and deals with treatment of metabolic syndrome. For this purpose complex treatment, which includes antihypertensive diet, individually selected aerobic physical exercise, introduction of metformin in dose 500 mg two days per day and melaxen, is carried out. Melaxen is introduced before going to bed 30 minutes before sleeping in dose 1-2 mg during first two weeks, and then in dose 3 mg during the following ten weeks. Course of treatment constitutes 12 weeks.
EFFECT: complex of non-pharmacological and pharmacological therapy as well as empirically selected mode of melaxen introduction ensure efficient treatment due to essential reduction of body weight and reduction of insulin-resistance in said group of patients.
SUBSTANCE: invention refers to medicine and concerns a method of treating insulin resistance. A peripherally effective amount of melanocortin receptor-4 agonist is introduced in a patient. The melanocortin receptor-4 agonist represents cyclic peptides containing the core sequence His-D-Phe-Arg-Trp-Cys.
EFFECT: invention provides effective treatment of insulin resistance by the peptide melanocortin receptor-4 agonist in the peripheral introduction.
34 cl, 11 dwg, 13 tbl
SUBSTANCE: newborn rabbits' pancreases are placed in a salt solution with an antibiotic at temperature 4-10°C and grounded microfragments. Further they are incubated in a free medium from serum at first incubation temperature 36.6 to 37°C, during a first incubation period with periodic replacement of a serum-free medium and removal of spontaneously collapsed undesired cells until at least 80% of the remained cells represent beta cells. It is followed by incubation in the serum-free medium periodically replaced at second incubation temperature 22 to 29°C, during a second incubation period until at least 78-90 % of the remained cells represent beta cells.
EFFECT: invention allows producing beta cells applicable for transplantation to patients with diabetes for stimulating natural insulin development.
4 cl, 40 tbl, 14 ex
SUBSTANCE: invention refers to medicine. A method involves in the fact that autologous cell cultures of patient's fibroblasts enriched with multipotent mesenchymal stem cells (MMSCs) are once transplanted on a wound surface. A portion of the culture MMSCs makes 5 to 15%. The cells are applied on the wound dropwise and fixed on the tissue defect with the use of a fibrine carrier and protected with a bandage made of platelet-rich plasma.
EFFECT: method provides reduced length of treatment; it is safe and high-effective.
2 dwg, 1 tbl, 1 ex
SUBSTANCE: invention relates to medicine, namely to endocrinology and can be used for reduction of hypoglycemia acute exacerbation or severe hypoglycemia exacerbation in patients with type II diabetes after treatment with insulin. For this purpose vildagliptin or its salt is introduces to patient in combination with insulin.
EFFECT: invention ensures reduction of risk of hypoglycemia development, as well as necessity to apply several antihyperglycemic medications.
12 cl, 1 tbl, 1 ex
SUBSTANCE: invention refers to medicine and concerns an osmotic delivery system promoting Exenatid release at a constant speed varying within approximately 5 mcg/day to approximately 160 mcg/day. The present invention also concerns a method of treating Insulin-independent diabetes in a subject and a method of treating obesity in a subject.
EFFECT: invention provides improved therapeutic consistency, higher clinical effectiveness, improved quality of life for the subjects recovered (in comparison with the injection therapy), and enabled immediate treatment interruption by removing the osmotic delivery system.
15 cl, 5 ex, 5 dwg, 5 tbl
FIELD: medicine, pharmaceutics.
SUBSTANCE: group of inventions relates to field of medicine and deals with medication for per oral treatment of diabetes mellitus and other diseases, accompanied by disturbance of tolerance to glucose, and method of obtaining such medication. Medication contains antibodies to beta-subunit of insulin receptor in activated form, obtained by multiple successive dilution and external impact in accordance with homeopathic technology. Method of obtaining hard drug form for per oral treatment of diabetes mellitus and other diseases, accompanied by disturbance of tolerance to glucose, includes mixing efficient amount of carrier, irrigated in pseudo-liquefied layer with water-alcohol dilution of antibodies in activated form to beta-subunit of insulin receptor, prepared by combination of multiple successive dilution - reduction of antibodies concentration and external impact according to homeopathic technology, and dried at temperature not higher than 35°C, with pharmaceutically acceptable additives and further tabletting of mixture by direct dry compressing.
EFFECT: medication can be used for efficient treatment and prevention of diabetes mellitus and other diseases, accompanied by disturbance of tolerance to glucose.
5 cl, 6 ex
FIELD: medicine, pharmaceutics.
SUBSTANCE: invention relates to medicine and pharmaceutical industry, and deals with diuretic composition with delayed release, in which torasemide is an active substance.
EFFECT: compositions, corresponding to invention, are used to obtain possibility to avoid trouble-causing pressing urge to urinate, caused by conventional compositions with immediate release.
9 cl, 11 ex, 2 tbl, 5 dwg