Method of production of fluorescent labels based on biodegradable nanoparticles of silicon for use in vivo
SUBSTANCE: invention relates to the field of nanomaterials. A method is proposed for production of fluorescent labels based on biocompatible and biodegradable nanoparticles of silicon for application in vivo by the reaction of disproportionation of silicon monoxide at a temperature of 950°C in air atmosphere followed by interaction of the resulting nanoparticles of silicon with dimethylsulfoxide. The proposed method enables to produce two outfits of nanoparticles with the size of 2.0-2.5 nm with photoluminescence peaks at 676 nm and 774 nm.
EFFECT: production of hydrophilic biocompatible and biodegradable fluorescent labels of nanocrystalline silicon, having sustained bright luminescence and narrow function of size distribution, resistant to high temperatures (up to 220°C) without the use of toxic substances in the process of their synthesis; resulting nanoparticles are applicable as labels in vivo in imaging of deep-seated tissues and organs.
1 cl, 3 dwg
The invention relates to the field of nanotechnology and nanomaterials, the method of obtaining fluorescent labels based on biocompatible and biodegradable silicon nanoparticles for in vivo applications, allowing you to receive a mass quantity of nanomaterial.
Currently one of the most common approaches to solving this problem is the use of semiconductor quantum dots based on CdSe, CdTe. The wavelength of maximum fluorescence depends on the kernel size and nature of the semiconductor. This requirement satisfies the following semiconductors: CdSe, CdTe, PbSe, PbS, InP. Several yielding the best fluorescent labels in the quantum fluorescence yield (~70% at room temperature), nanocrystals exceed by several orders of magnitude in the values of the absorption cross-section of the exciting light. As a result, the brightness of the nanocrystals is so high that can detect single objects using a standard fluorescent microscope.
Recently developed methods of synthesis photostabilizer nanocrystals InP, InP/ZnS, but the low brightness of the fluorescence and a wide distribution of particle sizes limit their use in biochemistry. The disadvantages of CdSe, CdTe, PbSe, PbS, InP, InP/ZnS toxicity is also included in the composition of the quantum dots ions that will require building a special protective shells. Suspicious attitude toward the use of quantum dots in vivo was recently expressed in a number of works: by ICP MS was prodemonstrirovano accumulation Kanovich points in the body of the animal after administration of intravenous. In addition, nanometer size crystals can lead to passive or active transport and accumulation in cell organelles, leading to unpredictable delayed effects. Therefore, the search for quantum dots with alternative chemical composition and do not contain toxic ions, is extremely important. In this regard, of particular interest are quantum dots based nanogramme.
The closest to the essential features of the claimed method of obtaining fluorescent labels based on biodegradable silicon nanoparticles for in vivo applications is the method proposed in [Park, J.-H., Gu L., von Maltzahn g, Ruoslahti E., S.N. Bhatia, and M.J. Sailor Biodegradable luminescent porous silicon nanoparticles for in vivo applications // Nature materials. 2009. V.8, №4. P.331-336]. In this paper, fluorescent nanoparticles of porous silicon was obtained by the method of electrochemical etching of a silicon substrate in an alcohol solution of hydrofluoric acid, the removal of the porous silicon film, followed by sonication and the allocation obtained porous silicon nanoparticles by filtration through a membrane with the size of the om 0.22 μm pores. To activate fluorescence obtained porous nanoparticles were incubated in water for two weeks. Immediately after forming the porous layer, dangling bonds of silicon on the surface is then passivated mainly hydrogen, which is replaced by oxygen. During the stage of activation, treated with hydrogen since the capacity of silicon oxide. Such structures exhibit strong luminescence associated with defects localized at the interface of silicon and silicon dioxide.
The disadvantages of the invention are: a large particle size is 126 nm and modal distribution of the spectral intensity of fluorescence in the field of 650-900 nm, which does not allow spectral encoding fluorescent labels with different wavelengths of fluorescence in different proportions.
The technical result of the present invention is a method of obtaining a hydrophilic biocompatible and biodegradable fluorescent labels nanocrystalline silicon with a stable bright luminescence with an intensity peaks in the area of 676 nm and 774 nm, resistant to high temperatures (up to 220°C) without the use of toxic substances in the process of their synthesis.
This technical result is achieved due to the fact that to obtain hydrophilic fluorescent the data labels use the disproportionation of silicon monoxide at a temperature of 950°C in air, followed by the interaction of nanoparticles of silicon with dimethylsulfoxide, leading to the formation of two ensembles of nanoparticles with average sizes of 2.0-2.5 nm and a narrow distribution in size from 1.3 to 4.0 nm, having a photoluminescence maxima at 650 nm and 730 nm.
Use the following disproportionation fine silicon monoxide at a temperature of 950°C. to obtain nanocrystalline silicon:
where n is the number of silicon atoms in the silicon nanoparticles (n=29 for silicon nanoparticles of diameter of 1.0 nm; n=286 for silicon nanoparticles of a diameter of 2.0 nm), followed by dissolution and leaching of silica in the acidified aqueous solution.
The formation of nanocrystalline silicon occurs in the thickness of the crystals formed during the sintering of oxide of silicon, similar to the decomposition of solid solutions that effectively protects nanocrystalline silicon from oxidation by air. However, annealing in air leads to the formation of impurity centers (Si=O) and (Si-O-Si), energy levels which lie within the bandgap of the silicon nanoparticles of a size of 2.0-2.5 nm, which allows to shift the maximum of fluorescence required for the fluorescent labels red region of the spectrum. Immediately after annealing see the luminescence of the obtained nanogramme when exposed to UV radiation in the red region of the spectrum.
For stabilizat and and hydrophilization the surface of the silicon nanocrystals annealed mixture in a Teflon beaker pour the concentrated hydrofluoric acid, warmed up slightly and placed in an ultrasonic bath for intensification etching. After dissolution products at 313K solid particles precipitated by centrifugation and washed 2 times with ethanol. Received luminescense sediment Usacheva and transferred into a quartz tube containing dimethyl sulfoxide. The tube is heated to 150°C for complete evaporation of ethanol, again Usacheva in an ultrasonic bath and quickly heated to the boiling point of dimethylsulfoxide (~189°C). During 30 to see the darkening mist and a significant increase in the brightness of a luminescence. The process can be described by the following reaction equation:
where m is the number of hydrogen atoms on the surface of nanoparticles (m<n; m=24 for silicon nanoparticles of diameter 1.0 nm). The basis of Zola are hydrophilic silicon nanoparticles in dimethyl sulfoxide, which is known to be non-toxic substance. Obtained by coagulation of a colloidal solution of silicic nanopure depending on the modes of reaction may consist of particles with a size of up to several nanometers in diameter and has a definite crystalline structure of the Central core (Figure 1 and 2).
Silicon nanoparticles with a size of 2.0-2.5 nm have own intensive luminescence in the orange-red region of the spectrum with maximum and 676 nm and 774 nm (Figure 3). Quantum yield of fluorescence obtained biometar nanoparticles is more than 15%.
From Figure 3 it is evident that the proposed method of producing a fluorescent biometar gives two ensembles of particles danocrine having a fluorescence maximum at 676 nm (curve 2) and 774 nm (curve 3). Fluorescent labels having the luminescence spectrum at different wavelengths allow you to implement a spectral encoded microparticles, which opens the way for the development of test-systems for rapid multi-parameter analysis of a large number of biological objects based on microarray technique. One of the advantages of use as fluorophores NC with different wavelengths issue is the possibility of excitation of all coding components with a single monochromatic source. To obtain a solution of fluorescent labels sample of the original solution is dried under reduced pressure and diluted with distilled water.
The development of a fairly simple method of obtaining a biocompatible and biodegradable fluorescent labels based on nanocrystalline silicon, with a bright stable photoluminescence in the visible spectral range in mass quantities opens the possibility of their application in medicine and biology for fluorescent diagnostics, photodynamic who photothermal therapy photochemical sterilization of the blood supply, as well as in ecology for water purification from organic pollutants and pathological microflora.
The unique optical properties of danocrine, such as photostability and a wide range of bands of fluorescence depending on the diameter of the core nanoparticles, including in the near-infrared range, make them attractive for use as in vivo markers for imaging superficial tissues and organs. Another option is to use the obtained fluorescent labels - flow cytofluorimetry, allowing to analyze the spectral properties of each passing through the detector nanoparticles. The use of spectral encoded nanoparticles involves the analysis of each fluorescent label for detection of the presence in the analyzed sample of each of the detected objects.
This invention will find wide application in medicine for the diagnosis of tumors of various types. For example, for imaging of deep tumors in vivo wavelength excitation fluorescence nanoparticles of silicon must be selected in the near-red region of the spectrum in order to obtain maximum absorption of radiation cloth and minimum absorption chromophore such proteins, such as hemoglobin.
The method according to the teachings of fluorescent labels based on biocompatible and biodegradable silicon nanoparticles for in vivo applications, characterized in that for obtaining hydrophilic fluorescent labels use the disproportionation of silicon monoxide at a temperature of 950°C in air, followed by the interaction of nanoparticles of silicon with dimethylsulfoxide, leading to the formation of two ensembles of nanoparticles with a size of 2.0-2.5 nm, with the maximum photoluminescence at 676 nm and 774 nm.
SUBSTANCE: invention relates to methods of producing crystalline aluminosilicates which enable to satisfy demands which use said aluminosilicates for their direct purposes in industrial production, namely: electrochemical, chemical, as well as devices for implementing such types of technologies. The method involves placing starting material inside a container insulated from its surrounding medium, and exposing said material to a physical field generated in order to convert said material to the end product, said exposure being carried out immediately in its zone of influence, wherein the object for carrying out the latter is an air suspension of sand particles containing silicon and aluminium oxides with particle size of 1-8 mcm, and total volume of these particles relative the total volume of the inside of the container which they fill is 20-40% of its total value, and the physical field used is a rotating alternating magnetic field whose strength, which is measured in the treatment zone, is equal to 2.5×103-1×106 A/m, and frequency is equal to 40-70 Hz, and the container filled with the treated material acts as the closing connecting link for the flux formed in its and generated by the magnetic system used, and during treatment, a jet of compressed air at excess pressure of 0.1-0.6 kgf/cm2 is fed into the bottom residue obtained during said treatment, and the time interval over which such conversion of the starting material to end products takes place is equal to 12-17 minutes.
EFFECT: reduced expenses during synthesis of crystalline aluminosilicates; the apparatus used when realising this method is characterised by simple design, resulting in its high operational reliability.
2 cl, 1 dwg
FIELD: process engineering.
SUBSTANCE: invention can be used for production of ultrapure silicon. Initial material melt containing SiO2 with stibium, mercury and sulfur is subjected to electrolysis to obtain silicon solution. Obtained silicon solution is rinsed by solvents that dissolve easily elementary sulfur to remove the latter. Residue produced after rinsing at stage b) is treated by strong acid to remove dopant ions. Then precipitate is treated by reducing agent to reduce mercury and/or stibium salts. Separation by specific weight is performed to separate silicon from other components.
EFFECT: ultrapure silicon with specific resistance of up to 8000 Ohm·cm.
13 cl, 5 ex
SUBSTANCE: mixture of silicon-containing compounds with gas-carrier is fed by the coaxial tube equipped with central electrode with both tube and electrode being made from pure silicon. The central electrode is connected to the potential fan of the high frequency resonance transformer with capacity 1-1000 kW and voltage 10-1000 kV. At temperature 700-9000°C plasma discharge lights up with frequency 1-800 kHz in the jet of silicon-containing compounds between central electrode and coaxial tube walls as well as between central electrode and substrate-target located axisymmetrically to the central electrode.
EFFECT: decrease of energy consumption and of side-products yield during polycrystalline silicon production, enhancing of environmental safety.
113 cl, 8 dwg, 2 ex
SUBSTANCE: method includes separation of silicium tetrafluoride by fluorination of natural silicium oxides - silica sand or quartzite - with reversible elementary fluorine, electrolytic reduction of melted eutectic of fluoride salts of alkaline metals and washing of electrolyte salts and impurities with ammonium hydrofluoride.
EFFECT: invention allows obtaining semiconducting polycrystalline silicium with reduced expenses.
SUBSTANCE: invention refers to nanostructured materials with ultra-fine grain structure, and namely two-phase alpha-beta titanium alloys which can be used for manufacture of semi-finished products and products in different branches of engineering, machine-building industry and medicine. Proposed alloy has microstructure consisting of ultra-fine grains of alpha-phase and beta-phase with the size of less than 0.5 mcm. In alloy microstructure the amount of grains with grain shape coefficient of not more than 2 is not less than 90%; at that, more than 40% of grains have wide-angle borders, and average density of dislocation is not more than 1014 m-2. the method for obtaining ultra-fine grain two-phase alpha-beta titanium alloy involves heat treatment with heating of a billet at the temperature of not more than 0.6 T"пп", further multicycle intense plastic deformation with achievement of accumulated true deformation degree e≥4. Then, plastic deformation is performed so that the billet shape is changed at the rate of less than 10-1 s-1 in several cycles to provide deformation degree ε≥50%.
EFFECT: improving strength and fatigue properties and preserving high ductility.
5 cl, 2 dwg, 1 tbl, 1 ex
SUBSTANCE: invention relates to a biodegradable highly filled thermoplastic composition used in making films and consumer packaging. The composition contains polyethylene, biodegradable filler in form of potato starch, process additives: oligoepoxy ether with molecular weight of 1800-3500 and content of epoxy groups of 2.0-4.0% in nano-form and nonionic and cationic surfactants.
EFFECT: obtained composition has good process parameters, articles made from the composition are biodegradable under the effect of light, moisture and soil microflora.
2 tbl, 6 ex
SUBSTANCE: invention can be used in electronics, material science, instrument-making, metrology, information technology, chemistry, ecology, biology and medicine. Initial product of electric arc synthesis is separated into light and heavy fractions by distillation in "boiling layer" mode or by floatation. For "boiling layer" mode air is used as carrier-gas and is supplied at rate, which provides mode of laminar flow of light fraction. Sodium nitrate solution with specific weight 1.26-1.37 g/cm3 is used as floatation liquid. Heavy fraction is processed with concentrated hydrochloric acid and ultrasound, washed, dried and successively oxidised with air oxygen at 450 °C and 600 °C with intermediate processing with concentrated hydrochloric acid and ultrasound, washing and drying until constant weight is obtained.
EFFECT: reduction of process duration, improved quality of separated nanotubes due to reduction in them of metal and amorphous carbon.
4 dwg, 3 ex
SUBSTANCE: invention can be used for obtaining carbon nanotubes and nanofibres. Solid disperse catalyst is periodically loaded into reactor, gases are injected and subjected to contact with catalyst particles at the temperature of carbon nanomaterial synthesis. Loading of each following portion of catalyst is performed on the layer of growing nanomaterial. Alternate pressure of gaseous medium is created in reactor by injection of gases when pressure in reactor increases and their discharge, when pressure decreases. Stages of injection of gases into reactor and discharge of gases from reactor are performed by repeated periodical creation of vacuum in lock-chamber.
EFFECT: simplification of hardware process realisation, increased productivity due to uniform catalyst distribution.
2 cl, 2 dwg, 2 ex
SUBSTANCE: invention relates to method of obtaining nanocomposites based on polyolefins, used in obtaining different products, such as films, sheets, tubes, threads and fibres. Carbon nanotubes are preliminarily ground in water with addition of water-soluble polymer with concentration 0.01-0.1 wt %. After that, suspension is dispersed by ultrasound at maximal medium temperature not higher than 70° C. After that, suspension is applied on the surface of polyolefic granules and dried. Obtained granules of nanocomposite contain to 0.5 wt % of carbon tubes.
EFFECT: nanocomposite materials possess high volumetric and superficial electroconductivity, heat-conductivity and high rigidity, with simultaneous increase of tensile modulus of elasticity to 50%, and limit of tensile strength to 30%.
3 cl, 4 dwg, 3 tbl
SUBSTANCE: invention relates to field of chemical technology of obtaining paints and varnishes. Composition for giving surface properties of self-cleaning based on lotus effect includes hydrophobising component amides or esters of perfluoropolyoxalkylene-sulfo- or perfluoropolyoxalkylene-carboxylic C17-46 acids, dissolved in organic solvent Freon, isopropanol or their mixture, structure-forming component, selected from the group: organosoluble silica sol with particle size 3-18 nm, tetrabutoxytitanium, tetraisopropoxytitanium, tetraethoxysilane or products of its partial hydrolysis, with weight ratio of hydrophobising and structure-forming components within 100:(4-7) and concentration of hydrophobising component in solvent 0.2-8 wt %.
EFFECT: invention provides self-cleaning properties of processed surface.
FIELD: process engineering.
SUBSTANCE: invention relates to production of thin-film materials base on system of double oxides used fast developing areas of electronic lighting engineering, etc, as sensitive, decorative, filtering and distributing coatings, etc. Proposed method comprises preparing film forming solution and applying it on article surface. Fresh film forming solution is kept for 8-13 days at 6-8°C, drying is performed at 60°C for 30-40 min and nonlinear heating to 800-900°C in air and curing at 800-900°C for one hour and cooled in conditions of natural cooling down of muffle furnace at the following ratio of components in film forming solutions in wt %: tetraethoxysilane - 22.6-21.4; hydrochloric acid - 4.4-10-4-1.2-10-4, distilled water - 2.3-0; metal salt - NiCl2-6H2O 1.1- 8.4; ethanol - (98 wt %) making the rest.
EFFECT: simplified production of uniform coating with perfected properties.
2 ex, 1 dwg
SUBSTANCE: invention relates to field of heterogenic catalysis, in particular, to method of obtaining catalyst for isotopic protium-deuterium exchange. Method includes obtaining metal nanoparticles in reduction of metal ions in inverse micellar solution, consisting of solution of metal salt, representing RhCl3 or RuOHCl3, SAS, representing sodium bis(2-ethylhexyl)sulfosuccinate, and non-polar solvent, isooctane, with further application on Al2O3 carrier, with silver nanoparticles being obtained by preparation of inverse micellar rhodium or ruthenium solutions with ratio of molar quantity of metal salt water solution to molar quantity of SAS in the range from 1:1 to 10:1; after that water-alcohol solution in quantity 5-50 wt % and ammonium solution in quantity 10-30 wt % are added, after which suspension is subjected to ultrasound processing, deaeration and exposure to γ-irradiation 60Co with dose from 1 to 40 kGy.
EFFECT: invention makes it possible to obtain catalyst which possesses high catalytic activity and is intended for work in temperature interval 77÷400 K.
2 cl, 4 tbl, 4 ex
FIELD: process engineering.
SUBSTANCE: invention relates to synthesis of inorganic sorbents to be used in water treatment, radio-chemistry and processing if liquid radioactive wastes. Proposed method comprises processing of porous carrier selected from glass powder, active coal or silica gel by water suspension of sorption active insoluble compound selected from the group including nickel ferrocyanide, aluminium hydroxide and copper sulphide at ultrasound radiation of 50-200 W and 22-60 kHz and at temperature of 70-90°C.
EFFECT: mechanically active inorganic sorbents with high content of active substance.
3 tbl, 3 ex
SUBSTANCE: invention refers to medicine, namely to orthopaedic dentistry. A method for making an intraosseous dental implant involves sand-blast finish of an implant surface by alimunium oxide particles, plasma-based layer-by-layer deposition of a system of biocompatible coatings containing mixed titanium or titanium hydride or calcium hydroxyapatite powders onto an implant carrier. The first layer consists of titanium or titanium hydride of dispersity 3-5 mcm at spraying distance 70-80 mm and thickness 5-10 mcm. The second layer is titanium or titanium hydride of dispersity 50-100 mcm at spraying distance 100 mm and thickness 50-115 mcm. The third layer represents mixed titanium or titanium hydride of dispersity 40-70 mcm and calcium hydroxyapatite of dispersity 5-10 mcm in ratio 60-80 and 20-40 wt % respectively, at spraying distance 80 mm and layer thickness 15 -20 mcm. The forth layer consists of calcium hydroxyapatite of dispersity 40-70 mcm at spraying distance 70 mm and layer thickness 20-30 mcm. Thereafter, the multilayered system of the biocompatible coatings is covered by a metal film consisting of a ferric triad (iron, cobalt or nickel) of thickness 20-35 nm by magnetron sputtering. A carbon nanocoating of thickness up to 1 mcm is prepared thereon. The carbon nanocoating represents carbon nanotubes and carbon nanofibres of diameter 50-200 nm.
EFFECT: method provides making the implant coated so that to promote the active growth of bone tissue.
3 cl, 1 tbl, 4 dwg
SUBSTANCE: invention relates to medicine, namely to endoscopy and can be used for diagnostics of neoplastic changes of digestive tract mucosa. For this purpose endoscopic examination of mucosa is performed in accordance with the standard methods. Localisation and spread of altered mucosa sections are visually determined. After that, non-fluorescent dye is applied on said mucosa sections. Then, spectrophotometric examination is carried out. Indigo carmine is used at wavelength of laser irradiation 532 nm, indigo carmine or Congo red is used if examination is performed at wavelength 632 nm.
EFFECT: method makes it possible to carry out spectrophotometric examination in limited zone of isolated section of pathologically changed mucosa due to application of certain dyes, which do not distort endogenic fluorescence, which in its turn, increases accuracy of spectrophotometric measurements and reliability of diagnostics.
3 cl, 1 ex
SUBSTANCE: invention refers to a contrast medium for ultrasonic imaging in the form of a preparative form. The declared medium contains microparticles a solid coating of which is presented in the form of a stabilizing formulation containing fine lecithin and polyethylene glycol of molecular weight 6000, filled with sulphur hexafluoride in the specific proportions. The preparative form may be suspended in a physiologically acceptable liquid suspension medium in a dose of 0.025-2.0 mg of the microparticles per 100 g of animal's body weight.
EFFECT: invention provides the improved ultrasonic images for at least 5 minutes, higher echogenicity of the analysed tissue.
5 cl, 2 ex
SUBSTANCE: visualisation agent contains a conjugate of formula (I) of benzopyrylium dye through a linker group with a 3-100-dimensional synthetic peptide which provides directed delivery to the biological target. Also disclosed is a pharmaceutical composition which contains said conjugate of formula (I), a set for preparing said pharmaceutical composition and methods for visualisation of a mammal body in vivo.
EFFECT: invention provides efficient visualisation of a mammal body in vivo while monitoring diseased state thereof.
24 cl, 5 tbl, 6 ex
FIELD: medicine, pharmaceutics.
SUBSTANCE: group of inventions refers to medicine and may be used for treating Philadelphia chromosome positive leukaemia (Ph+ leukaemia). A method of treating chronic myeloid leukaemia (CML) or Philadelphia chromosome positive acute lymphoblast leukaemia (Ph+ALL) in a group of human patients involves the stages as follows: (a) introducing the pre-determined fixed amount of imanitib or a pharmaceutically acceptable salt thereof in human patients in need thereof, (b) selecting at least one blood sample in the specified patients for at least first three months of the therapy, (c) determining minimal plasma imanitib (Cmin), and (d) specifying a dose of imanitib or the pharmaceutically acceptable salt thereof so that Cmin falling within the range of 1000 ng/ml and 3000 ng/ml is achieved in each patient wherein threshold Cmin 1000 ng/ml has not been achieved before specifying the dose. The group of inventions also involves the use of imanitib or the pharmaceutically acceptable salt thereof for preparing a medicine for treating Ph+ leukaemia.
EFFECT: group of inventions enables minimising or avoiding the formation of resistance, loss of effectiveness and a risk of recurrence in human patients suffering CML or Ph+ALL and treated with imanitib or the pharmaceutically acceptable salt thereof.
14 cl, 8 dwg, 2 tbl, 2 ex
FIELD: medicine, pharmaceutics.
SUBSTANCE: invention refers to a magnetic system which has a structure containing magnetic nanosized particles of formula MIIMIII 2O4, wherein MII=Fe, Co, Ni, Zn, Mn; MIII=Fe, Cr, or maghemite which are functionalized by bifunctional compounds of formula R1-(CH2)n-R2 where n = 2-20, R1 is specified in: CONHOH, CONHOR, PO(OH)2, PO(OH)(OR), COOH, COOR, SH, SR; R2 is an external group, and is specified in: OH, NH2, COOH, COOR; R is an alkyl group or an alkaline metal specified in C1-6-alkyl and K, Na or Li, respectively). The structure also includes a polymer optionally containing a pharmacologically active molecule, and an external pharmacologically active molecule may be specified in anticancer agents, antimicrobial agents, anti-inflammatory agents, immunomodulators, molecules acting on the central nervous system or able to mark cells so as to enable the identification thereof by means of the usual diagnostic detectors. The invention also refers to a method for preparing the nanosized particles of formula MIIMIII 2O4 which consists in adding a metal salt to alcohol, heating to complete solubilisation of salts, adding water to facilitate salt hydrolysis and heating to temperature 150-180°C to prepare a suspension to be then functionalised. The invention also refers to a method for preparing the magnetic system, wherein the functionalised nanoparticles and the pharmacologically active molecules are integrated into the matrix of a water-insoluble polymer, and the prepared structure is continuously and one-stage coated with adequate surfactants.
EFFECT: invention aims at preparing the magnetic system suitable for hyperthermia procedures.
16 cl, 4 tbl, 1 dwg, 26 ex
FIELD: medicine, pharmaceutics.
SUBSTANCE: invention refers to medicine, and may be used for preparing a composition for diagnosing/monitoring of a patient suffering from a disease state mediated by activated macrophages. What is used is a composition comprising a conjugate or a complex of general formula Ab-X wherein the group Ab contains a ligand binding folate receptor which binds to activated macrophages. The group X contains an imaging agent wherein the ligand binding folate receptor is conjugated to the imaging agent by γ-carboxyl group of glutamic acid, of the ligand binding folate receptor.
EFFECT: enabled diagnosing or monitoring of the following diseases: rheumatoid arthritis, ulcerative colitis, Crohn's disease, skin infections, osteomyelitis, atherosclerosis, organ transplant rejection, pulmonary fibrosis, sarcoidosis, scleroderma, and chronic inflammation, due to the imaging agents within locations of the populations of activated macrophages and inside of them.
17 cl, 15 dwg, 19 ex
SUBSTANCE: invention relates to medicine, traumatology, orthopedics in pediatrics, rehabilitation. Into cavity of pathologically changed hip joint (HJ) under combined anesthesia introduced is 0.9% sodium chloride solution until tight filling with involuntary 10-20° bending of patient's leg in HJ, 10-15° abduction and external 5-10° rotation. After plain radiography 1.5-5 ml of contrast - 60% urographin are introduced into HJ cavity and forced movements in HJ are performed for contrast to penetrate into all its parts. After that, multispiral computed tomography with 1.2 mm thick axial cuts is performed. On obtained scanograms in frontal plane determined are: degree of femur (F) head dislocation relative to acetabulum (A), A state by cartilage reference points, roof, anterior and posterior edges of A, dimensions of acetabular lip, anterior-posterior and vertical dimensions of A, its depth, indices of its spatial location by angle of vertical A inclination and angle of frontal inclination. Degree of A filling with contrast determined are: presence of fat pad hypertrophy, presence of scar tissues in A projection, deformation of its cartilage edges and acetabular lip, vertical and anterior-posterior dimensions of F head, its form is determined with assessment of deformation degree, spatial orientation of F neck by true neck-shaft angle and anteversion angle, and F condyles must be strictly in frontal plane. State of HJ capsule, state and location of F head ligament, inconsistency between F head and A, volume of A fat pad hypetrophy, presence of scar tissues are estimated. Results are used to determine possibility of closed reposition of F into A or surgical elimination of HJ dislocation by arthrotomy, ablation of scar tissues, straightening acetabular lip with reposition of F head into A.
EFFECT: complex estimation of all mentioned indices ensures complete assessment of state of growing cartilage structures of HJ in children, true spatial A orientation, proximal part of hip, reliable detection of anatomical obstacles for reposition of F head into A in order to determine tactics of further treatment.
3 cl, 2 ex