Method of effluents treatment

FIELD: process engineering.

SUBSTANCE: invention relates to biochemistry. Effluents are cleaned of suspended substances, oil products, phenols and chlorides for water to be discharged into pool. Inner surface of filtration dam is processed by bacterial culture Pseudomonas fluorescens "ВКГ" RCAM 00538 with titre of 10-13-10-11 in amount of 30 mg/dm3 in dry weight to obtain biofilm. Said filtration dam is filled with water to be cleaned and kept therein for at least 3 days. Effluents are forced through filtration dam consisting of the following rocks: Crushed stone or sand-gravel mix, or mix of mudstone with siltstone.

EFFECT: efficient cleaning to MPC acceptable for water discharge into pool.

1 dwg, 14 tbl, 18 ex

 

The invention relates to biotechnology wastewater using microorganisms, and is intended in particular for wastewater treatment coal and oil drilling water with industry to improve sanitary conditions of water bodies receivers.

For wastewater treatment in the coal industry the most frequently used clarifiers and filtration units. But often traditional cleaning methods do not provide the required quality of treated water for discharge into water bodies. Wastewaters from coal mining, and oil drilling waste water industry contain suspended solids, oil products, phenols, minerals, and their purification from oil products, phenols, minerals requires significant material costs.

A known method of purification of waste water from oil products and phenols using microorganisms in the aeration tank (J. Microbiology, No. 2, 1985, article "the Use of associations of bacteria when cleaning podslantsevyh water from oil, p.12-15). When using this method of purification from suspended solids is carried out before application of wastewater in the aeration tank. The use of aeration in the specified method entails costs as the supply of oxygen to intensify the cleaning process, and the costs of pre-clearance from vesvese the different substances.

A known method of purification of natural water and groundwater, including aeration followed by filtration through several layers of filtering media: granite gravel, peat, bark, gravel and sand, and the volumetric ratio of consecutive layers is 1:2:3:2:1. The total height of the filter load is 0.8-1.2 m (Patent RF №2151105, IPC C02F 1/64, C02F 1/28). This method is primarily intended for treatment of wastewater from iron.

The use of aeration in the known method requires additional costs, which increases the cleaning process. In addition, almost purification from oil products, phenols, and trace elements does not occur. Therefore, treated water discharge into water bodies require significant additional costs for the cleanup process.

The known method for wastewater treatment by the drainage of filter pads, which is filtering the waste water through the draining mineral soil with the chemical reactions between the dissolved salts and special substances introduced into the filter material and forming insoluble compounds. They provide currency toxic to soil salt components into harmless ions, which in some cases are necessary reclaiming elements (e.g., calcium), in improving the bottom-physical and agrochemical properties of soils. Thus, as the filter material, it is recommended to use sand and special additives, lime, and IONEX chips (low-grade ion exchangers or waste production. After performing its functions, the drain filter pad eliminated by filling mineral soil and soil when conducting mining reclamation of land used for oil or slurry is discharged into the barn, followed delaborated its contents by known methods. (Shemetov VY Technique and technology of purification of drilling wastewater. M VNIIEM, 1990. 48 C.)

However, the waste water that has passed such cleaning does not meet the requirements of environmental regulations on a range of indicators, for example, the content of chlorides.

Closest to the proposed technical solution is the method of purification of sewage by using as a filter loading of rocks (sandstones, mudstones and siltstones), i.e. the waste of coal mining. During the filtration of wastewater with solids content of 200-300 mg/DM3that is clean to 1-2 mg/DM3respectively (J.V. Lesin. Filters for water purification from large waste of coal mining. J. Coal. Safety, industrial sanitation, environmental protection., No. 2, 1988). The filters have a simple construction, the size of the trading in natural or artificial cavities (ravines, logs, old mine workings and the like). In this case, the filter array width is limited by the walls of the grooves, and the lower recess being constructed water retention dam for collecting clarified water. The known method is simple in operation. The process of sewage treatment in this case is capable of self-regulation. Sedimentation in the lower layers of the filter array in the cleaning process leads to an increase of the water level in the receiving waters, resulting engage the upper, fresh layers of filter array, which is put on top. Known purification process does not require significant costs (they are commensurate with the cost of dumping).

However, the disadvantage of this method is the treatment of wastewater only from suspended solids. Waste water is free from suspended solids discharged into the reservoir contains oil products, phenols, chlorides, that does not solve the problem of cleaning them against the full range of pollutants. So for discharge into the pond wastewater coal industry and drilling wastewater oil industry, in which the content of oil products, phenols, chlorides and trace elements exceed the MPC requires additional cleaning and significant costs.

The objective of the invention is to develop a method that provides the treatment at the same time from TOD is decayed substances, oil products, phenols, chlorides with the lowest cost, both physical and material.

The technical result is the simultaneous treatment of wastewater from suspended substances, oil products, phenols, chlorides for treated water discharge into water bodies.

The above technical result is achieved in that in the method of purification of waste water from suspended substances, oil products, phenols, chlorides, including the filtering of water through the filter dam of rocks, according to the invention create a biofilm on the inner surface of the filter dam, by processing its bacterial strain Pseudomonas fluorescens BKFRCAM00538 with title 10-13-10-11after treatment bacterial strain, filter dam fill waste water, and kept in her water for at least 3 days, and then pass the wastewater through the filter dam, and as the rocks take the gravel or sand-gravel mixture, or a mixture of claystone to siltstone.

In addition, the bacterial strain Pseudomonas fluorescens BKTRCAM00538 with title 10-13-10-11for handling charge in the amount of 30 g/l of dry matter.

Bacterial strain Pseudomonas fluorescens WKH deposited in the collection of the GSI research Institute for agricultural Microbiology (Saint-Petersburg) 05.07.2011 under registration number RCAM00538.

The stated set of beings is the R signs "...create a biofilm... bacteriolysis strain of Pseudomonas fluorescens WKH RCAM00538 with title 10 -13-10-11...provides education on the inner surface of the filter dam biofilms, which allows you to clean waste water from the contained suspended solids, oil products, phenols, chlorides to the MPC values corresponding to discharge into the pond.

In the patent and scientific literature unknown solutions containing elements similar to declare, therefore, the proposal meets the criterion of "novelty". Also for the first time, on the basis of the developed method achieved the provision of wastewater suspended solids, oil products, phenols, chlorides simultaneously to the appropriate norms for discharge into the reservoir at minimum cost, i.e. the claimed technical solution meets the criterion of "inventive step".

For carrying out the proposed technical solutions for wastewater purification from suspended substances, oil products, phenols, chlorides in natural conditions of mine "Korkino", section "Korkino", ICBM "Alanko", and ICBM "Catlick" was created by a skilled site. Figure 1 presents the scheme of purification of waste water, where 1 is the drainage ditch, 2 - pond to the dam, 3 - the pond after the dam, 4 - filter dam, 5 - discharge of treated water.

From the main wastewater stream was dug by a bulldozer drainage ditch 1 width 1.0 m, Globino is 0.3 to 0.4 m, built filter dam of rocks with the following dimensions: length of the lower base - 2.4 m, the length of the upper base - 1.3 m width 3.7 m, depth 0.4 m, and the lower 2 and upper pond 3.

Wastewater to filter the dam 4 is served by the drainage ditch 1 by gravity. The pond 2 before filtering dam plays the role of a device for uniform water supply and distribution over the cross section of the filter dam. The pond 3 after the dam is required to collect and discharge of treated water. In the drainage ditch 5 is the discharge of water after cleaning, and then into the pond receiver.

Wastewater treatment took place in the filter dam of rocks, which used the gravel or sand-gravel mixture, or a mixture of claystone to siltstone. On the inner surface of the filter dam was established biofilm, by processing its bacterial strain Pseudomonas fluorescens BKT RCAM00538 with title 10-13-10-11isolated from mine water in laboratory conditions. The microorganisms were adsorbiroval on the particles of crushed stone, sand and gravel, a mixture of claystone with silt, forming a biofilm. Filtering the dam was maintained in static conditions for at least 3 days, after which, translated into dynamic conditions and carried out the filtering process.

In the construction of the dam was determined as the slope of the impermeable layer i, affecting n is the rate of water movement through the filter dam:

i=(HI-Ho)/ LH=(0,30-0,29)/2,4=0,0042, (1)

where NI=0.3 m and No=0,29 m - point elevations of the water inlet and outlet of the filter dam, defined on the place;

LH=2.4 m - length of the lower base of the filter dam.

The cross-sectional area of the filter dam Sdwas calculated based on its size: width=3.70 m and depth h=0,4 m:

Sd=B×h=3,70×0,40=1,48 m2(2)

Volume of filter dam is:

Vd=Sd(LH+LB) /2=1/mtext> ,48(2,4+1,3)/2=2,74 m3(3)

where: LB- the upper base of the filter dam.

The water flow rate (Qd) through the filter dam was determined on the basis of cross-sectional area Sdand filtration coefficient K=1,55 m/h (gravel); K=3,17 m/h (the dog-Cano gravel); K=0.25 m/h (a mixture of claystone to siltstone):

Qd=Sd×K=1,48×1,55=2,3 m3/h(gravel)(4)

Qd=4.7 m3/h (sand and gravel)

Qd=0,37 m3/h (mixture of claystone to siltstone). The average residence time of wastewater in the filter loading is:

t=(LH+LB)/2K=(2,4+1,3) /(2×1,55)=1,2h(yebenb)(4)

t=0,58 h (sand and gravel)

t=7.4 hours (mixture of claystone with silt).

Wastewater treatment in pilot test was conducted with the above calculated data.

Sampling of wastewater on chemical and microbiological analyses were performed:

- prior to the filtration dam (raw water);

after filtration of the dam (purified water).

Microbiological studies in the process of water purification included the determination of the activity of microorganisms of the genus Pseudomonas.

Chemical water analysis included determination of pH potentiometrically at PND f 14.1:2:3:4.121-97 KHA water.

The content of suspended substances - FR. 1.31.2002.00670 MIM mass concentrations gravimetric method.

The oil content in the effluent - method X on PND f 14.1:2.5-95 KHA water.

The content of chlorides in wastewater according to GOST 26428-85.

The content of phenols - FR. 1.31.2002.00650 MIM mass concentrations using 4-amidopirina.

The content of microelements on PND f 14.1:2:3:4.135-98 KHA waters of measuring mass concentration of metals by atomic-amiss the Onna spectrometry with inductively coupled plasma.

The inventive method is implemented as follows.

Example 1.

The flow path of the discharged mine sewage mine "Korkino" was built filter dam of rubble with fractional composition:

d=4,5-5,5 mm - 70%;

d=3,0-4,0 mm - 20%;

d=1,0-2,5 mm - 7%;

d=0,3-0,5 mm to 3%.

The composition of source water are presented in table 1.

The filter dam of gravel processed bacterial strain Pseudomonas fluorescens WKH RCAM00538 with title 10-13isolated from mine water, in the amount of 30 g/l of dry matter of the calculation of 3.6 liters per 1 m3volume filter dam. Then filter the dam was filled with sewage water and was maintained in static conditions for at least 3 days, during which the title has not been changed, resulting in the inner surface of the dam formed a biofilm. After 3 days of filtering the dam was put into operation in dynamic mode.

After running the filter dam samples were taken of the effluent mine water for chemical and microbiological analysis prior to the filtration dam after dam.

The flow of water through the dam was equal to 2.3 m3/h filtration coefficient of 1.55 m/h

The average residence time of wastewater in the filtration dam - 1,2 hours

The control was performed in a similar dike with gravel, but without processing by microorganisms. Experience and control was carried out at the rate which the water temperature of 18-19°C.

The results of the tests are presented in table 1.

The table shows the test results of wastewater through the filter dam (proposed method) and the results of the tests in the control. The table shows that the amount of suspended solids in the experiment decreased with 240,20 to 0.8 mg/DM3in control - with 298,75 to 11,98 mg/DM3in the prototype up to 1-2 mg/DM3. Experience has decreased oil from 0.30 to 0.02 mg/DM3, phenols with 0.05 mg/DM3up to 0.001 mg/DM3. chloride - 473,17 to 230,0 mg/DM3.

There was a decrease in the number of AI, Ba, In, Wa, Fe, K, CA, Mg, Mn, Cu - several times. To control the amount of oil products, phenols, chlorides and these trace elements remained on the same level as before treatment. In the prototype purification was achieved only from solids.

Thus, purification of mine waters of the proposed method through the filter dam of gravel processed bacterial strain Pseudomonas fluorescens WKH RCAM00538 with title 10-13allows discharge of treated water into the pond.

Example 2. The proposed method is analogous to example 1 with the same parameters and conditions, in the same filter dam of rubble clearing subjected to waste water career section "Korkino".

The results of the tests are presented in table 1. The amount of suspended solids decreased with 320,40 to 0.5 mg/DM 3the amount of oil products from 0.55 to 0.03 mg/DM3, phenols from 0.04 to 0.001 mg/DM3, chlorides with 398,17 to 236,0 mg/DM3the number of trace elements decreased several times.

In control (section "Korkino") clearance was only suspended substances with 287,5 to 9.6 mg/DM3.

Thus, quarry waters of the proposed method through the filter dam of gravel processed bacterial strain Pseudomonas fluorescens WKH RCAM00538 with title 10-13allows discharge of treated water into the pond.

Example 3. On mine "Korkino" was built filter dam of sand and gravel with fractional composition:

d=4,5-5,5 mm - 45%;

d=3,0-4,0 mm - 20%;

d=1,0-2,5 mm - 15%;

d=0,3-0,5 mm - 15%;

d=0.1-0.25 mm - 5%.

Composition of mine waters are presented in table 2.

Filter dam of sand and gravel was processed bacterial strain Pseudomonas fluorescens WKH RCAM00538 with title 10-13and was maintained during 3 days in static conditions, and then waste mine water mine "Korkino" was passed through the filter dam filtration coefficient - 3,17 m/h

The test results presented in table 2. The amount of suspended solids in the filtration decreased from 184,6 to 1.2 mg/DM3products from 0.1 to 0.04 mg/DM3, phenols with up to 0,001 0,012 mg/DM3the number of gloriousness with 387,8 to 210,0 mg/DM 3a number of trace elements in several times. In the control there was only a reduction of suspended solids with 184,53 to 18.76 mg/DM3.

Thus, waste mine water water method proposed through the filter dam of sand and gravel processed bacterial strain Pseudomonas fluorescens WKH RCAM00538 with title 10-13allows the discharge of treated water into the pond.

Example 4. The proposed method is analogous to example 3 with the same settings and conditions in the filter dam of sand and gravel cleaning subjected to waste water career section "Korkino".

The results are presented in table 2.

In the experience of the amount of suspended solids decreased after treatment with 193,6 to 0.9 mg/DM3; petroleum 6,07 to 0.05 mg/DM3; phenols of 0.015 to 0.001 mg/DM3; chlorides with 347,6 to 75,0 mg/DM3and a number of trace elements in 2.0-1.5 times. In control has occurred only clean from suspended solids with 179,53 to 1.85 mg/DM3that is only due to filtering. From the rest of the ingredients (oil products, phenols, and trace elements) cleaning was not observed.

Thus, the waste of quarry waters of the proposed method through the filter dam of sand and gravel processed bacterial strain Pseudomonas fluorescens WKH RCAM00538 with title 10"13allows spookydan water in the pond.

Example 5. On mine "Korkino" was built filter dam of a mixture of claystone to siltstone with fractional composition:

d=5,5-6,5 mm - 85%;

d=4,5-5,0 mm - 9%;

d=3.5-4.0 mm - 3%;

d=2,0-3,0 mm - 2%;

d=0,5-1,5 mm - 1%.

Analogously to example 1 filter dam of a mixture of siltstone shale sections were treated bacterial strain Pseudomonas fluorescens WKH RCAM00538 with title 10-13selected from mine waters in the amount of 30 mg/DM3on the dry matter and matured for 3 days under static conditions, and then waste mine water mine "Korkino" was passed through the filter dam filtration coefficient - 0.25 m/h

The test results presented in table 3.

In the experience of the amount of suspended solids decreased from 260,78 to 1.36 mg/DM3in the control 274,81 to 1.8 mg/DM3. The number of petroleum products decreased from 3.44 to 0.03 mg/DM3, phenols with from 0.005 to 0.001 mg/DM3the number of chloride decreased with 328,7 to 228,0 mg/DM3the number of trace elements in several times.

In the control was observed only clean from suspended solids. From oil products, phenols and micronutrients cleaning was not observed.

Thus, waste mine water the proposed method through the filter dam of a mixture of claystone to siltstone treated bacterial strain Pseudomonas fluorescens WKH RCAM00538 with title 10-13on the allows to reset the purified water in the reservoir.

Example 6. Waste water career with the section "Korkino" passed through the filter dam of a mixture of siltstone shale sections with the same parameters and conditions as in example 5.

The test results presented in table 3.

In the experience of the amount of suspended solids decreased with 228,51 to 1.13 mg/DM3the number of petroleum products decreased from 3.25 to 0.03 mg/DM3, phenols from 0.08 to 0.001 mg/DM3, chlorides with 148,5 to 84,9 mg/DM3the number of trace elements (AI, BA, V, W, Fe, K, CA, Mg, Mn, Cu) decreased 1.5-1.8 times. In the control was observed purification from suspended substances with 234,57 to 1.5 mg/DM3other ingredients remained at the same level.

Thus, quarry waters water proposed method through the filter dam of a mixture of claystone to siltstone treated bacterial strain Pseudomonas fluorescens WKH RCAM00538 with title 10-13allows discharge of treated water into the pond.

Example 7. Waste mine water mine "Korkino" passed through the filter dam of rubble with the same fractional composition that in example 1, but to handle the filtering of the dam took the bacterial strain Pseudomonas fluorescens WKH RCAM00538 with title 10-11in the amount of 30 mg/DM3on the dry substance. Within 3 days mine water was in the dam under static conditions. After 3 days the title remained the same is smoother, the cleaning mode translated in dynamic conditions with the filtration coefficient of 1.55 m/h and the flow rate of 2.3 m3/PM

The test results presented in table 4. The result of the filter, the amount of suspended solids decreased from 212,75 to 1.34 mg/DM3products from 0,48 to 0.04 mg/DM3, phenols from 0.013 to 0.001 mg/DM3, chlorides with 473,17 to 233,0 mg/DM3the number of micronutrients on average decreased 1.5 times. To control the amount of suspended solids decreased from 207,25 to 11,95 mg/DM3other ingredients remained in control at the same level.

Thus, purification of mine waters of the proposed method through the filter dam of gravel processed bacterial strain Pseudomonas fluorescens WKH RCAM00538 with title 10-11allows discharge of treated water into the pond.

Example 8. Career water cut "Korkino" passed through the filter dam of rubble with the same fractional composition as in example 1. To handle the filtering of the dam took the bacterial strain Pseudomonas fluorescens WKH RCAM00538 with title 10-11in the amount of 30 mg/DM3on the dry substance. Within 3 days of quarry water was in the dam under static conditions. After 3 days the titer remained at the same level, the cleaning mode translated in dynamic conditions.

The test results presented in table 4.

In d is the query result of filtering the amount of suspended solids decreased from 110,20 to 1.2 mg/DM 3products with 0.70-0.05 mg/DM3, phenols of 0.015 to 0.001 mg/DM3, chlorides with 398,17 to 300,0 mg/DM3the number of trace elements decreased on average 1.3 times.

To control the amount of suspended solids decreased from 97,06 to 6.87 mg/DM3other ingredients in the control virtually remained unchanged.

Thus, quarry waters the proposed method allows the discharge of treated water into the pond.

Example 9. Waste mine water mine "Korkino" passed through the filter dam of sand and gravel with the same fractional composition that in example 3, but to handle the filtering of the dam took the bacterial strain Pseudomonas fluorescens WKH RCAM00538 with title 10-11in the amount of 30 mg/DM3on the dry substance. Within 3 days mine water was in the dam under static conditions. After 3 days the titer remained at the same level, the cleaning mode translated in dynamic conditions with permeability 3,17 m/h

The test results presented in table 5.

In the experience of the amount of suspended solids decreased after treatment with amounts to 188.7 to 1.67 mg/DM3the number of petroleum products decreased from 3,62 to 0.05 mg/DM3, phenols with from 0.005 to 0.001 mg/DM3the number of trace elements decreased several times.

To control the amount of suspended solids decreased from 193,26 on the 11,51 mg/DM 3. The rest of the ingredients (oil products, phenols and trace elements) remained at the same level.

Thus, purification of mine waters of the proposed method through the filter dam of sand and gravel processed bacterial strain Pseudomonas fluorescens WKH RCAM00538 with title 10-11allows discharge of treated water into the pond.

Example 10. Through the filter dam of sand and gravel with the same parameters and conditions as in example 4, but to handle the filtering of the dam took the bacterial strain Pseudomonas fluorescens WKH RCAM00538 with title 10-11in the amount of 30 mg/DM3on the dry substance, missed a waste of quarry water cut "Short-Kinsky". The test results presented in table 5.

After cleaning, the amount of suspended solids decreased from 180,77 to 1.9 mg/DM3The amount of oil products from 1.96 to 0.05 mg/DM3, phenols with up to 0.011 0.001 mg/DM3, chlorides with 147,6 to 115,0 mg/DM3the number of trace elements decreased by an average of 1.0-1.2 times.

To control the amount of suspended solids decreased with 187,12 to 12.0 mg/DM3. Other ingredients remained at the same level,

Thus, treatment of waste (career) water proposed method through the filter dam of sand and gravel processed bacterial strain Pseudomonas fluorescens WKH RCAM00538 with title 10-1 allows discharge of treated water into the pond.

Example 11. Through the filter dam of a mixture of siltstone shale sections with the same fractional composition that in example 5, but to handle the filtering of the dam took the bacterial strain Pseudomonas fluorescens WKH RCAM00538 with title 10-11in the amount of 30 mg/DM3on the dry substance, missed mine waste water from the mine "Korkino". The test results presented in table 6.

The amount of suspended solids amounted to clean 255,72 mg/DM3after purification of 1.55 mg/DM3the number of petroleum products; to clean 3,26 mg/DM3after cleaning - 0.05 mg/DM3the number phenols; to clean - 0.006 mg/DM3after cleaning 0.001 mg/DM3the number of chlorides; to clean - 336,6 mg/DM3after cleaning 296,0 mg/DM3the number of trace elements decreased on average by a factor of 1.2.

To control the amount of suspended solids decreased from 265,19 to 1.77 mg/DM3. The quantity of oil products, phenols and minerals remained at the same level.

Thus, treatment of waste (mining) water proposed method through the filter dam of a mixture of claystone to siltstone treated bacterial strain Pseudomonas fluorescens WKH RCAM00538 with title 10-11allows discharge of treated water into the pond.

Example 12. Through the filter dam of claystone with what levaitom with the same fractional part, as in example 6 was passed quarry water cut "Korkino". We used bacterial strain Pseudomonas fluorescens WKH RCAM00538 with title 10-11in the amount of 30 mg/DM3on the dry substance. In this example, the purification from suspended substances, oil products, phenols, chlorides and trace elements were less efficient than when using the same bacterial strain, but with a titer of 10-13.

The amount of suspended solids decreased from 232,65 to 1.40 mg/DM3products from 3.18 to 0.04 mg/DM3, phenols with up to 0,001 0,006 mg/DM3, chlorides with 350,5 to 300,0 mg/DM3. We also observed a decrease in the number of micronutrients on average 1.2 times.

To control the amount of suspended solids decreased from 241,15 to 1.62 mg/DM3. For the rest of the ingredients, the decrease was not observed.

Thus, treatment of waste (career) water proposed method through the filter dam of a mixture of claystone to siltstone treated bacterial strain Pseudomonas fluorescens WKH RCAM00538 with title 10-11allows discharge of treated water into the pond.

Studies have shown that when using a bacterial strain with a titer of less than 10-13and more than 10-11the cleaning process from the above ingredients remained on the same level, not raised when the titer of less than 10-13was inalsa when the titer of more than 10 -11.

Thus, the amount of suspended solids in waste mine and quarry waters under the influence of biofilms formed as a result of processing filter dam bacterial strain Pseudomonas fluorescens WKH RCAM00538 with title 10-13in the clearing process through the filter dam of rubble in the course of 1.2 h decreased significantly compared with control. When this happened purification from suspended solids below norms (10,55 mg/DM3) 0.8 mg/DM3In the prototype up to 1-2 mg/DM3.

There was a decrease in the number of petroleum products from 0.30 to 0.02 mg/DM3(MAC - 0.05 mg/DM3), phenol - 0.05 mg/DM3up to 0.001 mg/DM3(MPC - 0.001 mg/DM3), chlorides with 473,17 up to 300 mg/DM3(MPC - 300 mg/DM3).

When filtering waste mine water cut "Korkino" the amount of suspended solids decreased with 320,40 to 0.5 mg/DM3(MPC - 10,55 mg/DM3), the amount of oil products from 0.55 to 0.03 mg/DM3(MAC - 0.05 mg/DM3), the amount of phenols from 0.004 to 0.001 mg/DM3((MAC - 0.001 mg/DM3), chlorides with 398,17 up to 75 mg/DM3(MPC - 300 mg/DM3).

A number of trace elements fell into a sewage pit and quarry waters in 2-3 times.

There was a decrease in suspended solids, oil products, phenols and minerals in the mining and quarry waters when using the dam from claystone to siltstone and food is the length of cleaning 7.4 hours, also to norms. When using a dam of sand and gravel occurred purification from suspended substances, oil products, phenols, and trace elements during 0,58 PM

Table 7 summarizes the comparative evaluation of cleaning mine and mine water during the filtration through the filter dam of crushed stone or sand and gravel or a mixture of claystone to siltstone treated bacterial strain with a titer of 10-13and 10-11.

The results of the experiments were compared with the prototype. The table shows that purification from suspended substances in bacterial titer of 10-13is more intensive than the titer of 10-11as in mine and quarry waters. The most intensive purification from suspended solids was achieved by filtration through the dam, loaded with gravel, covered with biofilm bacterial strain with a titer of 10-13. Purification from oil products, phenols, chlorides was also more intense when the titer of 10-13than title 10-11.

The duration of treatment in the dam of rubble was 1.2 h at the filtration coefficient K=1,55 m/h; from the sand-and-gravel - 0,58 h when K=3,17 m/h of a mixture of claystone to siltstone - 7.4 hours when K=0.25 m/h

Purification from suspended solids in all three versions of filtration through the dam with crushed stone, sand and gravel mixture and a mixture of claystone with silt, dormancy is itih the biofilm, was intense, the amount of suspended solids after treatment was less than MPC (10,55 mg/DM3).

The quantity of oil products, phenols, chlorides in all three variants did not exceed the MCL (0.05 mg/DM3oil and 0.001 mg/DM3- phenols, chlorides 300 mg/DM3).

Example 13.

Through the filter dam of rubble with the same fractional composition that in example 3 was passed drilling waste water from the site ICBM "Alan-Kul". We used bacterial strain Pseudomonas fluorescens WKH RCAM00538 with title 10-13and 10-11in the amount of 30 g/DM3on a dry weight basis. Then filter the dam was filled with sewage water and was maintained in static conditions for at least 3 days, during which the title has not been changed, resulting in the inner surface of the dam formed film. After 3 days of filtering the dam was put into operation in dynamic mode.

After running the filter dam was selected samples of the drilling mud chemical and microbiological analysis prior to the filtration dam after dam.

The average duration of the drilling fluid in the filtration dam - 0,46 PM

The control was performed in a similar dam of rubble, but without processing by microorganisms. Experience and control was carried out at a temperature of drilling mud 18-19°C.

The results tested the th are presented in table 8.

The table shows the test results of waste drilling water through the filter dam (proposed method) and the results of the tests in the control.

The results of the experiment were compared with the prototype. The table shows that the amount of suspended solids in the experience fell from 2196 mg/DM3to 0.2 mg/DM3in the control 2196 mg/DM3up to 150.7 mg/DM3. Experience with bacterial titer of 10-11the weighted number decreased to 0.4 mg/DM3.

The amount of oil in the experience (bacterial titer of 10-13) decreased from 4,55 to 0.04 mg/DM3. Experience with bacterial titer of 10-11the number fell 4,55 to 0.05 mg/DM3.

To control the amount of oil actually did not fall.

The amount of chlorides in wastewater MBR "Alan-cool when cleaning using biofilms of strain Pseudomonas fluorescens WKH RCAM00538 with title 10-13decreased from 16886,8 mg/DM3to 290,0 mg/DM3and in waste water with bacterial titer of 10-11with 16886,8 mg/DM3to 296,3 mg/DM3.

In the control treatment from chloride was not observed, the amount of chlorides remained almost at the same level -16886,8 mg/DM3.

A number of trace elements in waste water ICBM "Alan-cool declined several times.

Thus, it was noted cleanup of drilling wastewater, the proposed act shall obom from suspended solids, oil, chlorides, which allows the treated water discharge into water bodies.

Example. 14. The results of the test drilling wastewater from land-based ICBMs "Alan-Kul" through a dam of sand and gravel using biofilms of strain Pseudomonas fluorescens WKH RCAM00538 with title 10-13and 10-11presented in table 9. The amount of suspended solids in the experience with bacterial titer of 10-13fell from 2194,0 mg/DM3to 5.40 mg/DM3; bacterial titer of 10-11- to 9.50 mg/DM3. To control the amount of suspended solids decreased from 2194,0 mg/DM3up to 150 mg/DM3.

Cleaning of oil with a titer of 10-13it was noted with of 4.57 mg/DM3up to 0.04 mg/DM3. Experience with title 10-11purification from oil products recorded of 4.57 mg/DM3up to 0.05 mg/DM3. In the control purification from petroleum products was not observed, i.e. the number of petroleum products remained at the same level of 4.57 mg/DM3. The removal of the chlorides in the experience with title 10-13it was noted 16886,8 mg/DM3to 292,0 mg/DM3with a titer of 10-11- 300,0 mg/DM3. A number of trace elements in the experience with bacterial titer of 10-13and 10-11decreased several times.

Cleaning of oil, chlorides and trace elements in the control were observed.

Thus, it was noted cleanup of drilling wastewater before Agamim way from suspended solids, oil, chlorides, which allows the treated water discharge into water bodies.

Example 15. The results of drilling wastewater from land-based ICBMs "Alan-cool through the dam from a mixture of claystone to siltstone using biofilms of strain Pseudomonas fluorescens WKH RCAM00538 with title 10-13and 10-11presented in table 10.

When cleaning drilling waste water through the dam from a mixture of claystone to siltstone happened purification from suspended solids in the experiment with a strain of Pseudomonas fluorescens WKH RCAM00538 with title 10-13with 2195,0 mg/DM3up to 0.1 mg/DM3when the titer of 10-11to 0.2 mg/DM3. In the control purification from suspended solids was observed with 2195,0 mg/DM3to 194,8 mg/DM3.

Cleaning of oil in the experience with title 10-13happened with 4,56 to 0.05 mg/DM3. Experience with title 10-11cleaning of oil was observed with 4,56 to 0.05 mg/DM3. In the control purification from petroleum products was not observed.

From chlorides experience with title 10-13clearing occurred with 16885,7 mg/DM3to 293,0 mg/DM3. Experience with title 10-11the removal of the chlorides mentioned with 16885,7 mg/DM3to 295,0 mg/DM3. To control the amount of chlorides remained at the same level - 16885,7 mg/DM3. A number of trace elements in the experience with title 10-13and 10-11decreased several times. To control the number of microel the cops remained at the same level, before cleaning and after cleaning.

Thus, it was noted cleanup of drilling wastewater the proposed method from suspended solids, oil, chlorides, which allows the treated water discharge into water bodies.

Example 16. The results of drilling waste water through the dam from the rubble using biofilms of strain Pseudomonas fluorescens WKH RCAM00538 with title 10-13and 10-11on ICBMs "Catlick are presented in table 11.

Experience with title 10" the amount of suspended solids after purification came with 1975,2 mg/DM3to 0.0 mg/DM3with a titer of 10-11up to 0.2 mg/DM3. To control the amount of suspended solids decreased from 1975,2 mg/DM3to 974,5 mg/DM3.

The amount of oil in the experience with title 10-13decreased with 1.07 mg/DM to 0.04 mg/DM3with a titer of 10-110.05 mg/DM3. The amount of chlorides in the experience with title 10-13fell from 8678,5 mg/DM3to 292,0 mg/DM3. Experience with title 10-11with 8678,5 mg/DM3up to 300 mg/DM3. A number of trace elements in the experience with title 10-13and 10-11decreased several times.

To control the amount of oil, chlorides and minerals after treatment remained at the same level as before treatment.

Thus, it was noted cleanup of drilling wastewater the proposed method from suspended solids, oil,chlorides, allowing the treated water discharge into water bodies.

Example 17. The results of drilling wastewater MBR "Catlick" through a dam of sand and gravel using biofilms of strain Pseudomonas fluorescens WKH RCAM00538 with title 10-13and 10-11presented in table 12.

Purification from suspended solids using biofilms with title 10-13it was noted with 1975,0 mg/DM3to 0.0 mg/DM3and experience with title 10-11with 1975,0 mg/DM3to 0.2 mg/DM3. In the control 1975,0 mg/DM3to 197,4 mg/DM3. Cleaning of oil in the experience with title 10-13with 1.07 to 0.04 mg/DM3. Experience with title 10-11with 1.07 0.05 mg/DM3. To control the amount of oil products remained at the same level of 1.07 mg/DM3. The removal of the chlorides in the experience with title 10-13it was noted with 8678,5 mg/DM3up to 300 mg/DM3. Experience with title 10-11cleaning from chloride was observed with 8678,5 mg/DM3up to 300 mg/DM3. In the control treatment of wastewater from chloride was not observed. A number of trace elements decreased with title 10-13and 10-11in several times. Thus, it was noted cleanup of drilling wastewater the proposed method from suspended solids, oil, chlorides, which allows the treated water discharge into water bodies.

Example 18. The results of drilling waste in the d ICBM "Catlick" across the dam from a mixture of claystone to siltstone using biofilms of strain Pseudomonas fluorescens WKH RCAM00538 with title 10 -13and 10-11presented in table 13.

Purification from suspended solids in the experience with title 10-13it was noted when the content of them with 1912,0 mg/DM3to 0.0 mg/DM3and experience with title 10-11with 1912,0 mg/DM3up to 0.1 mg/DM3. In the control 1912,0 mg/DM3to 190,4 mg/DM3. Cleaning of oil in the experience with title 10-13from 1,02 to 0.04 mg/DM3. Experience with title 10-11from 1,02 to 0.05 mg/DM3. To control the amount of oil products remained at the same level of 1.02 mg/DM3. The removal of the chlorides in the experience with title 10-13it was noted with 8619,6 mg/DM3up to 300 mg/DM3. Experience with title 10-11cleaning from chloride was observed with 8619,6 mg/DM3up to 300 mg/DM3. In the control treatment of wastewater from chloride was not observed. A number of trace elements decreased with title 10-13and 10-11in several times. To control the number of trace elements remained at the same level. Thus, it was noted cleanup of drilling wastewater the proposed method from suspended solids, oil, chlorides, which allows the treated water discharge into water bodies.

In table 14 comparative evaluation of effluent water drilling ICBM "Alan-Kul and ICBM "Catlick" through the dam from the crushed stone, sand and gravel and a mixture of claystone with silt, covered with biofilm strain is Pseudomonas fluorescens WKH RCAM00538 with title 10 -13and 10-11.

The results of the experiments were compared with the prototype. The table shows that purification from suspended substances in bacterial titer of 10-13is more intensive than the titer of 10-11as in drilling wastewater MBR "Alan-cool and ICBMs "Catlick". The amount of suspended solids was after cleaning is less than in the prototype when using dam of gravel, sand-gravel mixtures and mixtures of claystone to siltstone.

The most intense purification from suspended substances was held in the dam of a mixture of claystone to siltstone. Purification from suspended solids was in MBR "Alan-Kul" in the title biofilms 10-13- 0.1 mg/DM3in ICBM "Catlick" - 0,0 mg/DM3and when the titer of 10-11accordingly 0.2 mg/DM3and 0.1 mg/DM3. MAC purification from suspended solids is 10,55 mg/DM3. The number of petroleum products in all variants was not more than MCL (0.05 mg/DM3The number of chlorides after cleaning in the dam proposed method was at the level equal to the MPC (300 mg/DM).

The proposed method can dramatically reduce the amount of suspended substances, oil products, phenols, chlorides and trace elements in waste drilling water. The use of biofilms of strain Pseudomonas fluorescens WKH RCAM00538 with title 10-13and 10-11dramatically reduces contamination of drilling wastewater before pulling them in water, or back into the borehole.

Thus, the proposed method can dramatically reduce the amount of suspended substances, oil products, phenols, chlorides in the effluents, such as with mines, cuts and drilling wastewater to reduce the volume of wastewater discharge into water at minimal cost.

The use of the invention allows to obtain socio-economic effect, consisting in the improvement of sanitary conditions of water bodies receivers wastewater by eliminating excess discharge of pollutants, and the economic effect by reducing capital and operational costs of wastewater treatment, does not require special equipment, additional personnel, material costs.

0,026
Table 1.
The results of the tests of wastewater treatment using filtration dam of rubble with a biofilm of strain Pseudomonas fluorescens BKT RCAM00538 with title 10-13(experience) and without biofilm (control)
IngredientThe contents of the ingredients in wastewater, mg/DM3
experiencecontrol
mine "Korkino"The section "Korkino"Mine "Korkino"The section "Korkino"
DPDPDPDP
1345678910
pHcompared to 8.268,05of 8.067,188,218,208,158,15
Suspended solids240,200,8320,400,5298,7511,98287,59,6
Oil 0,300,020,55<0,030,800,793,173,17
Phenols0,05<0,0010,04<0,0010,0030,0030,0820,080
Chloride473,17230,0398,17236,0473,16473,16398,17398,16
Aluminum0,1990,0390,0620,0380,2160,2150,0750,074
Barium0,0140,008being 0.0360,0290,0290,0290,027
Bor0,286of 0.1330,2560,1140,1720,1700,3060,305
Vanadium0,003<0,0010,0050,0010,0040,0040,0080,008
Ironamount of 0.1180,059to 0.1270,0900,2540,2530,1260,126
Potassium24,1110,5428,7116,2553,1553,1430,1830,18
Calcium65,0136,7060,15around 41.2842,36 42,3554,2654,26
Magnesium67,7530,3064,7936,5059,2459,2377,1277,11
Manganese0,1120,0450,0250,0350,1260,1250,2110,210
Copper0,0040,0010,0040,0010,0040,0040,0050,005
Legend: D - before cleaning, P - after cleaning

Table 2.
The results of the tests of wastewater treatment using filtration dam of sand and gravel with a biofilm of strain Pseudomonas fluorescens WKH RCAM00538 with a title-10sup> -13(experience) and without biofilm (control)
IngredientThe contents of the ingredients in wastewater, mg/DM3
experiencecontrol
mine "Korkino"the section "Korkino"mine "Korkino"the section "Korkino"
DPDPDPDP
1345678910
pH8,147,958,027,358,128,128,278,27
Suspended solids184,61,2193,60,9184,5318,76179,531,85
Oil0,10,046,070,053,203,193,623,60
Phenols0,0120,0010,0150,0010,0040,0040,0050,005
Chloride387,8210,0347,675,0387,6387,5147,6147,5
Aluminum0,1810,0350,184to 0.0320,178 0,178to 0.1270,126
Barium0,056being 0.036being 0.0360,0210,0570,0560,0410,040
Bor0,2170.104 gof) 0.1570,065of € 0.1950,1940,1740,174
Vanadium0,0120,0010,0080,0010,0220,0220,0070,007
Iron0,0850,010,0420,0080,2450,2440,2360,235
Potassiumto 28.5717,3031,16 10,1926,5826,5729,8529,84
Calcium53,4938,7444,75to 27.8852,1452,1338,9738,97
Magnesium57,8539,0658,1431,1564,2564,2549,8849,88
Manganese0,2140,0490,1260,0470,1780,1780,1640,163
Copper0,0040,0010,0060,0010,0040,0040,0040,004
Legend: D-before cleaning, P - after cleaning

0,047td align="center"> 0,005
Table 3.
The results of the tests of wastewater treatment using filtration dam of a mixture of claystone to siltstone with biofilm of strain Pseudomonas fluorescens WKH RCAM00538 with a titer of 10-13(experience) and without biofilm (control)
IngredientThe contents of the ingredients in wastewater, mg/DM3
experiencecontrol
mine "Korkino"the section "Korkino"mine "Korkino"the section "Korkino"
DPDPDPDP
1345678 910
pHby 8.228,198,148,118,158,158,128,12
Suspended solids260,781,36228,511,13274,811,8234,571,5
Oil3,440,033,250,033,053.04 from3,373,37
Phenols0,0050,0010,080,0010,040,040,050,05
Chloride328,7228,0148,584,9 328,7328,7148,5148.5
Aluminumof) 0.1570,0330,1440,0380,1480,1470,132of 0.133
Barium0,0390,0210,0420,035to 0.032to 0.0320,0510,050
Bor0,1640,0480,1580,0480,1560,1560,1480,148
Vanadium0,0050,0010,0080,0010,0040,0040,0060,006
Ironamount of 0.1180,0470,0210,3120,3130,1370,136
Potassium27,19to 9.3222,7114,0341,8541,8521,9521,95
Calcium46,2527.18 per30,8414,0740,1640,1540,7640,75
Magnesium54,1122,2562,7836,4861,7461,7354,6354,60
Manganese0,1580,0720,0840,0370,1410,1410,1250,123
Copper0,0010,0030,0010,0040,0040,0050,005
Legend: D - before cleaning, P - after cleaning

Table 4.
The results of the tests of wastewater treatment using filtration dam of rubble with a biofilm of strain Pseudomonas fluorescens WKH RCAM00538 with a titer of 10-11(experience) and without biofilm (control)
IngredientThe contents of the ingredients in wastewater, mg/DM3
experiencecontrol
mine "Korkino"the section "Korkino"mine " Korkino"the section "Korkino"
DPDPD PDP
1345678910
pH8,148,118,058,028,258,248,158,15
Suspended solids212,75of 1.34110,201,2207,2511,9597,066,87
Oil0,480,040,700,052,932,930,970,96
Phenols0,0130,0010,015 0,001of 0.0810,0800,0550,055
Chloride473,17233,0398,17300,0473,17473,17398,17396,15
Aluminum0,4950,034to 0.0320,0270,2160,2150,0850,085
Barium0,0040,0030,0350,0030,037being 0.0360,0410,040
Bor0,1150,0960,2420,1310,1650,1660,2270,227
Vanadium0,00 0,0010,0040,0010,0040,0040,0030,003
Iron0,0550,0420,1440,0950,2870,2870,1150,115
Potassium28,2918,7316,2512,0832,4532,4428,06to 28.05
Calcium56,2340,1850,8246,1540,7740,7745,1345,12
Magnesium62,1138,7958,0639,2448,6348,6367,29 67,29
Manganese0,1860,0880,0750,0560,1210,1210,1880,187
Copper0,0050,0010,0040,0010,0050,0050,0040,004
Legend: D - before cleaning, P - after cleaning

Table 5.
The results of the tests of wastewater treatment using filtration dam of sand and gravel with a biofilm of strain Pseudomonas fluorescens WKH RCAM00538 with title 10-11(experience) and without biofilm (control)
IngredientThe contents of the ingredients in wastewater, mg/DM3
experiencecontrol
mine is "Korkino" the section "Korkino"mine "Korkino"the section "Korkino"
DPDPDPDP
1345678910
pH8,118,098,148,108,158,158,108,10
Suspended solidsamounts to 188.71,55180,771,9193,2611,51187,12to 12.0
Oil3,62 0,051,960,053,173,163,413,40
Phenols0,0050,0010,0110,0010,0030,0030,0040,004
Chloride387,8285,0147,6115,0387,8387,8147,5147,5
Aluminumof € 0.1950,035of 0.1330,0380,1670,167was 0.138was 0.138
Barium0,0390,0260,041to 0.0320,0450,0450,0480,047
Bor0,2050,1740,1160,0970,1840,1840,1790,178
Vanadium0,0130,0010,0060,0010,0310,0300,0080,008
Iron0,087of 0.0810,0870,0760,2130,2130,2250,225
Potassium32,424,726,5216,3729,5229,5224,7124,70
Calcium48,2438,1140,6331,9239,1739,17 42,2542,25
Magnesium51,0540,037,5827,0957,1457,1451,1951,18
Manganese0,1190,0980,163to 0.1080,1610,1600,169has 0.168
Copper0,0050,0010,0060,0010,0040,0040,0030,003
Legend: D - before cleaning, P - after cleaning

Table 6.
The results of the tests of wastewater treatment using filtration dam of a mixture of claystone to siltstone with biofilm of strain Pseudomonas fluorescens WKH RCAM00538 with title 10-11(experience) and without bio is Lenka (control)
IngredientThe contents of the ingredients in wastewater, mg/DM3
experiencecontrol
mine "Korkino"the section "Korkino"mine "Korkino"the section "Korkino"
DPDPDPDP
1345678910
pH8,148,118,128,008,088,088,108,10
Suspended solids 255,721,55232,651,40265,191,77241,151,62
Oil3,260,053,180,043,623,613,47of 3.46
Phenols0,0060,0010,0060,0010,050,050,040,04
Chloride336,6296,0350,5300,0336,6336,6350,5350,4
Aluminum0,1660,035to 0.1080,04of) 0.157of) 0.1570,146 0,145
Barium0,0370,0280,0490,0440,0390,0390,0470,047
Borstrength of 0.1590,0950,1370,0880,1630,1630,1420,140
Vanadium0,0060,0010,0070,0010,0060,0060,0050,005
Iron0,1030,0690,1420,0480,3160,3160,2020,202
Potassium11,1615,0725,2615,0740,67 40,6725,1425,14
Calcium41,3432,2430,7821,19up 38.94up 38.9435,0935,08
Magnesium48,2826,1556,2434,0762,5562,5650,8950,88
Manganese0,1640,1470,1010,0640,1450,1450,1370,136
Copper0,0050,0010,0040,0010,0050,0050,0040,004
Legend: D - before cleaning, P - after cleaning

Table 7.
Comparative evaluation of waste water from the mine "Korkino" and cut "Korkino" through the dam from the crushed stone, sand and gravel and a mixture of claystone with silt, covered with a biofilm of strain Pseudomonas fluorescens WKH RCAM00538 with title 10-13and 10-11
IngredientCleaning timeMACthe placeholder
1,2 h0,58 h7.4 hours
rubblesand and gravela mixture of claystone to siltstone
minethe incisionminethe incisionminethe incision
10-1310-1110-1310-11 10-1310-1110-1310-1110-1310-1110-1310-11
2345678910111213141516
Weighted
nye substances
VA, mg/DM3
0,8of 1.340,51,21,21,671,61,91,361,551,131,410,551-2
Of petroleum
the products, mg/DM3
0,020,04 0,030,050,040,050,050,050,030,050,030,040,05
Phenols, mg/DM30,0010,0010,0010,0010,0010,0010,0010,0010,0010,0010,0010,0010,001
Chlorides, mg/DM3230,0233,0236,0300,0210,0285,075,0115,0228,0296,084,9,0300,0300,0
The cylinder is
UNT filtering (To), m/h
-1,553,170,250,035

Table 8.
The results of the test drilling wastewater using dam of rubble with a biofilm of strain Pseudomonas fluorescens WKH RCAM00538 with title 10-13and 10-11and without biofilm (control)
IngredientThe contents of the ingredients in wastewater MBR "Alan-cool, mg/DM3
experience (title 10-13)experience (title 10-11)control
DPPP
12345
pH7,277,137,157,24
Usues the nnye substance 2196,000,200,40to 150.7
Oil4,550,040,054,55
Chloride16886,80290,00296,3016886,80
Vanadium0,00890,00130,00140,0089
Iron total2,560,120,132,54
Potassium2232,1047,548,62232,10
Calcium1215,40163,70182,401215,10
Magnesium240,8038,8037,10240,79
Manganese 0,9810,109of € 0.1950,981
Copper0,0210,0010,0010,021
Arsenic0,3520,0180,0520,349
Sodium8052,6117,5117,48052,6
Lead0,0470,0050,0060,047
Chromium total0,0480,070,070,048
Zinc0,4050,0120,0090,404
Legend: D - before cleaning, P - after cleaning

Table 9.
The results of the of spitoni drilling wastewater using dam of sand and gravel with a biofilm of strain Pseudomonas fluorescens BKT RCAM00538 with title 10 -13and 10-11and without biofilm (control)
IngredientThe contents of the ingredients in wastewater MBR "Alan-cool, mg/DM3
experience (title 10-13)experience (title 10-11)control
DPPP
12345
pH7,297,187,217,29
Suspended solids2194,00of 5.409,50150,0
Oil4,570,040,054,56
Chloride16885,00292,00300,0016885,00
In the of Nadi 0,00930,00110,00130,0093
Iron total2,561is 0.1020,1392,561
Potassium2231,0045,7050,502231,00
Calcium1217,10174,90183,301217,10
Magnesium242,7030,74of 36.76242,70
Manganese0,9780,129is 0.2600,978
Copper0,0220,00090,0010,022
Arsenic0,3590,0330,0530,359
Sodium 8051,30115,6119,38051,30
Lead0,0440,00320,00520,043
Chromium total0,0490,00470,00560,049
Zinc0,4040,00970,010,404
Legend: D - before cleaning, P - after cleaning

control
Table 10.
The results of the test drilling wastewater using dam from claystone to siltstone with biofilm of strain Pseudomonas fluorescens BKT RCAM00538 with title 10-13and 10-11and without biofilm (control)
IngredientThe contents of the ingredients in wastewater MBR "Alan-cool, mg/DM3
experience (title 10-13)experience (title 10-11)
DPPP
12345
pH7,197,127,117,19
Suspended solids2195,00,10,2194,8
Oil4,560,050,054,56
Chloride16885,7293,0295,016885,7
Vanadium0,0910,0010,0010,090
Iron totalto 2.570,130,142,56
Potassiumto 230.8 49,7to 49.92230,8
Calcium1214,7169, 5mm186,41214,6
Magnesium241,938,739,5241,9
Manganese0,9720,1340,1760,972
Copper0,0230,0010,0010,023
Arsenic0,3550,0420,0510,354
Sodium8050,7115,4118,28050,7
Lead0,0450,0040,0040,045
Chromium total0,0480,043 0,0560,047
Zinc0,4050,010,010,404
Legend: D - before cleaning, P - after cleaning

Table 11.
The results of the test drilling wastewater using dam of rubble with a biofilm of strain Pseudomonas fluorescens WKH RCAM00538 with title 10-13and 10-11and without biofilm (control)
IngredientThe contents of the ingredients in wastewater MBR "Catlick", mg/DM3
experience (title 10-13)experience (title 10-11)control
DPPP
12345
pH7,207,19 7,177,2
Suspended solids1975,200,2974,5
Oil1,070,040,051,06
Chloride8678,5292,0300,08978,4
Vanadium0,0200,0010,0010,012
Iron total0,250,050,090,25
Potassium237,746,948,4237,7
Calcium249,8of 56.468,8249,8
Magnesium36,917,927,5 36,9
Manganese0,5660,0980,1070,566
Copper0,0860,0010,0010,086
Arsenic0,0510,027being 0.0360,051
Sodium864,096,1118,3864,0
Lead0,00580,00300,00410,0058
Chromium total0,00580,00250,00390,0058
Zincamount of 0.1180,00790,0085amount of 0.118
Legend: D - before cleaning, P - after cleaning

Table 12.
The results of the test drilling wastewater using dam of the dog-Cano gravel with biofilm of strain Pseudomonas fluorescens WKH RCAM00538 with title 10-13and 10-11and without biofilm (control)
IngredientThe contents of the ingredients in wastewater MBR "Catlick", mg/DM3
experience (title 10-13)experience (title 10-11)control
DPPP
12345
pH7,197,187,097,19
Suspended solids1975,000,2197,4
Oil1,070,040,05 1,07
Chloride8678,5300,0300,08678,5
Vanadium0,0210,0010,0010,021
Iron total0,240,020,010,23
Potassium237,543,447,9237,5
Calcium219,941,468,5219,9
Magnesium36,715,824,336,7
Manganese0,5620,0850,0990,561
Copper0,0880,00130,0010,088
M is slack 0,0530,0070,0140,053
Sodium864,472,597,5864,4
Lead0,00520,00270,00560,0052
Chromium total0,00560,00130,00250,0056
Zinc0,1170,010,010,117
Legend: D - before cleaning, P - after cleaning

td align="center"> 0,0039
Table 13.
The results of the test drilling wastewater using dam from claystone to siltstone with biofilm of strain Pseudomonas fluorescens BKT RCAM00538 with title 10-13and 10-11and without biofilm (control)
IngredientProvide the e ingredients in wastewater MBR "Catlick", mg/DM3
experience (title 10-13)experience (title 10-11)control
DPPP
12345
pH7,257,087,127,25
Suspended solids1912,000,1190,4
Oil1,020,040,051,02
Chloride8619,6300,0300,08619,6
Vanadium0,0220,0010,0010,022
Iron total 0,230,010,0150,23
Potassium237,049,549,8237,0
Calcium248,949,754,3248,8
Magnesium36,813,5of 21.936,8
Manganese0,5630,0910,1050,562
Copper0,0890,0010,0010,089
Arsenic0,0520,0090,0140,052
Sodium864,0of 87.3107,5864,0
Lead0,00510,00310,0051
Chromium total0,00580,00280,00320,0057
Zinc0,1160,0160,0090,116
Legend: D - before cleaning, P - after cleaning

Table 14.
Comparative evaluation of waste water from the site ICBM "Alan-cool and ICBM "Catlick" through the dam from the crushed stone, sand and gravel and a mixture of claystone with silt, covered with a biofilm of strain Pseudomonas flnorescens WKH RCAM00538 with title 10-13and 10-11
№ p/pIngredientCleaning timeMACPro totype
1,2 h0,58 h7.4 hours
rubble sand and gravela mixture of claystone to siltstone
"Alan-Kul""Catlick""Alan-Kul""Catlick""Alan-Kul""Catlick"
10-1310-1110-1310-1110-1310-1110-1310-1110-1310-1110-1310-11
12345678910111213141516
1Suspended solids, mg/DM30,20,400,2of 5.40,500,20,10,200,110,551-2
2Oil products, mg/DM30,040,050,040,050,040,050,040,050,050,050,040,050,05
3Chlorides, mg/DM3290,0296,3292,0300,0292,0300,0300300,0 293,0295,0300,0300,0300,0
4The filtration coefficient (K), m/h0,771,580,120,035

1. The method of purification of waste water from suspended substances, oil products, phenols, chlorides, including the filtering of water through the filter dam of rocks, characterized in that create a biofilm on the inner surface of the filter dam by processing its bacterial strain Pseudomonas fluorescens WKH RCAM00538 with title 10-13-10-11then fill the filter dam water to be treated and can withstand water in it for at least 3 days, after which the waste water is passed through the filter dam, and as the rocks take gravel, or sand-gravel mixture, or a mixture of claystone to siltstone.

2. The method according to claim 1, wherein the bacterial strain Pseudomonas fluorescens WKH RCAM00538 with title 10-13-10-11take in the amount of 30 mg/DM3on a dry weight basis.



 

Same patents:

FIELD: chemistry.

SUBSTANCE: invention relates to a method of altering immunomodulating properties of lipopolysaccharides of plague bacteria in vitro, which involves obtaining preparations of lipopolysaccharides (LPS) and mouse toxin (MT) Yersinia pestis with subsequent formation of a LPS-MT complex thereof. A modified form of LPS-MT is used as an inducer of synthesis of cytotoxins TNF-α and IFN-γ. To this end, a test sample is prepared, to which LPS is added in amount of 5 mcg (50 mcl from working dilution of 100 mcl/ml) and MT is added in amount of 50 ng (5 mcl from working dilution of 10 mcg/ml); the sample is then incubated for 30 min at 37°C. The volume of the sample in eppendorfs is then brought to 100 mcl with sterile buffered physiological solution of NaCl and the obtained mixture is added a tray dimple containing a culture of human monocyte cell line U-937 (1×106 cells in a dimple); the latter is cultured in a medium of PRMI 1640 with simultaneous double control. Further, 1, 4, 20 hours after the beginning of combined incubation of the preparations of LPS with monocytes, quantitative accounting of the synthesised cytotoxins is carried out, wherein change in the immunomodulating properties of LPS of plague bacteria in vitro is determined from the amount of cytotoxins produced and the dynamics of their synthesis.

EFFECT: invention enables to alter immunomodulating properties of lipopolysaccharides of plague bacteria in vitro, which enables to realise toxic potential of the endotoxin of plague bacteria.

2 cl, 8 dwg, 2 ex

FIELD: biotechnologies.

SUBSTANCE: strain Rhodococcus erythropolis 1-KP, extracted from podzol soil contaminated with oil from the territory of the Kolsky peninsula, having high speed of oil utilisation, is deposited in the Departmental Collection of Beneficial Microorganisms of Agricultural Purpose of the Russian Academy of Agricultural Sciences (RCAM) (GNU VNIISHM) under the number RCAM01142 and may be used for treatment of contaminated soils from oil.

EFFECT: improved quality of soil cleaning from oil.

3 tbl

FIELD: biotechnologies.

SUBSTANCE: strain pseudomonas citronellolis 48-U, having high speed of oil and diesel fuel recycling, is deposited in the Departmental Collection of Beneficial Microorganisms of Agricultural Purpose of the Russian Academy of Agricultural Sciences (RCAM) (GNU VIISHM) under the registration number RCAM 01441 and may be used for treatment of contaminated soils from oil and diesel fuel.

EFFECT: improved quality of soil cleaning from oil and diesel fuel.

3 tbl

FIELD: biotechnologies.

SUBSTANCE: strain Rhodococcus fascians 4-G, extracted from soil contaminated with black oil and sampled from the territory of a boiler plant located in the settlement Gorelovo, Leningrad region, is deposited in the Departmental Collection of Beneficial Microorganisms of Agricultural Purpose of the Russian Academy of Agricultural Sciences (RCAM) (GNU VNIISHM) under the number RCAM01140 and may be used for treatment of contaminated soils from oil.

EFFECT: invention makes it possible to increase quality of soil treatment from oil.

3 tbl

FIELD: biotechnologies.

SUBSTANCE: strain Pseudomonas aeruginosa RCAM01139 is proposed for decomposition of oil and diesel fuel.

EFFECT: strain is characterised by high efficiency in processes of oil and diesel fuel utilisation, which are the only sources of carbon and energy.

3 tbl

FIELD: biotechnologies.

SUBSTANCE: strain Micrococcus luteus PBT-1, having high catalase activity and aiding increasing productivity of the agroecosystem, participating in processes of transformation of organic remains of natural origin, is deposited in the Departmental Collection of Useful Microorganisms of Agricultural Purpose of the Russian Agricultural Academy (RCAM) (GNU VNIISHM) under the registration number RCAM 01016 and may be used in transformation of organic remains of natural origin.

EFFECT: invention makes it possible to increase productivity of an agroecosystem.

3 ex

FIELD: biotechnologies.

SUBSTANCE: strain Penicillium sp. PBT-2 extracted from samples of humic pod mixed with cereal straw and having polyfunctional properties and promoting increased productivity of agroecosystem, participating in processes of transformation of organic remains of natural origin, is deposited in the Departmental Collection of Beneficial Microorganisms of Agricultural Purpose of the Russian Academy of Agricultural Sciences (RCAM) (GNU VNIISHM) under the registration number RCAM 01017 and may be used in transformation of organic remains of natural origin.

EFFECT: improved productivity of agroecosystem.

3 ex

FIELD: biotechnologies.

SUBSTANCE: strain of nodule bacteria Bradyrhizobium japonicum 859 (VNIISHM No.24117) is proposed, used as a facility to produce a fertiliser for soya. When soya seeds are treated with the fertiliser produced on the basis of the specified strain Bradyrhizobium japonicum, the index of germination increases by more than 8%, and crop capacity increases by 17-30%.

EFFECT: higher germination of plants, improved crop capacity.

4 tbl

FIELD: process engineering.

SUBSTANCE: set of invention relates to water treatment. Proposed method comprises immobilisation of biomass bearing oil-oxidisers taken in effective amount into organic hydrophobic peat-based sorbent and drying it. Note here that said organic hydrophobic sorbent is prepared by low-temperature pyrolysis in vacuum of peat minced to 0.2-0.5 mm size at 210-250°C for 60-90 minutes. Said immobilisation is performed by adding sorbent into biomass suspension during its fermentation at retardation of oil-oxidising microorganisms growth. Note here that mass of added sorbent is 6-10 times larger than that of microorganisms contained in biomass suspension at the moment of sorbent application. Said oil-oxidising microorganisms represent yeast culture Candida maltosa All-Russian collection of industrial microorganisms Y-3446 or culture of bacteria Dietzia maris All-Russian collection of industrial microorganisms Ac-1824. Besides, this invention covers biosorbant for water treatment thus produced.

EFFECT: faster preparation, higher absorption capacity and efficiency.

2 cl, 4 ex

FIELD: process engineering.

SUBSTANCE: set of invention relates to water treatment. Proposed method comprises immobilisation of biomass bearing oil-oxidisers taken in effective amount into organic hydrophobic peat-based sorbent and drying it. Note here that said organic hydrophobic sorbent is prepared by low-temperature pyrolysis in vacuum of peat minced to 0.2-0.5 mm size at 210-250°C for 60-90 minutes. Said immobilisation is performed by adding sorbent into biomass suspension during its fermentation at retardation of oil-oxidising microorganisms growth. Note here that mass of added sorbent is 6-10 times larger than that of microorganisms contained in biomass suspension at the moment of sorbent application. Said oil-oxidising microorganisms represent yeast culture Candida maltosa All-Russian collection of industrial microorganisms Y-3446 or culture of bacteria Dietzia maris All-Russian collection of industrial microorganisms Ac-1824. Besides, this invention covers biosorbant for water treatment thus produced.

EFFECT: faster preparation, higher absorption capacity and efficiency.

2 cl, 4 ex

FIELD: biotechnologies.

SUBSTANCE: strain Rhodococcus erythropolis 1-KP, extracted from podzol soil contaminated with oil from the territory of the Kolsky peninsula, having high speed of oil utilisation, is deposited in the Departmental Collection of Beneficial Microorganisms of Agricultural Purpose of the Russian Academy of Agricultural Sciences (RCAM) (GNU VNIISHM) under the number RCAM01142 and may be used for treatment of contaminated soils from oil.

EFFECT: improved quality of soil cleaning from oil.

3 tbl

FIELD: biotechnologies.

SUBSTANCE: strain pseudomonas citronellolis 48-U, having high speed of oil and diesel fuel recycling, is deposited in the Departmental Collection of Beneficial Microorganisms of Agricultural Purpose of the Russian Academy of Agricultural Sciences (RCAM) (GNU VIISHM) under the registration number RCAM 01441 and may be used for treatment of contaminated soils from oil and diesel fuel.

EFFECT: improved quality of soil cleaning from oil and diesel fuel.

3 tbl

FIELD: biotechnologies.

SUBSTANCE: strain Rhodococcus fascians 4-G, extracted from soil contaminated with black oil and sampled from the territory of a boiler plant located in the settlement Gorelovo, Leningrad region, is deposited in the Departmental Collection of Beneficial Microorganisms of Agricultural Purpose of the Russian Academy of Agricultural Sciences (RCAM) (GNU VNIISHM) under the number RCAM01140 and may be used for treatment of contaminated soils from oil.

EFFECT: invention makes it possible to increase quality of soil treatment from oil.

3 tbl

FIELD: biotechnologies.

SUBSTANCE: strain Micrococcus luteus PBT-1, having high catalase activity and aiding increasing productivity of the agroecosystem, participating in processes of transformation of organic remains of natural origin, is deposited in the Departmental Collection of Useful Microorganisms of Agricultural Purpose of the Russian Agricultural Academy (RCAM) (GNU VNIISHM) under the registration number RCAM 01016 and may be used in transformation of organic remains of natural origin.

EFFECT: invention makes it possible to increase productivity of an agroecosystem.

3 ex

FIELD: biotechnologies.

SUBSTANCE: strain Penicillium sp. PBT-2 extracted from samples of humic pod mixed with cereal straw and having polyfunctional properties and promoting increased productivity of agroecosystem, participating in processes of transformation of organic remains of natural origin, is deposited in the Departmental Collection of Beneficial Microorganisms of Agricultural Purpose of the Russian Academy of Agricultural Sciences (RCAM) (GNU VNIISHM) under the registration number RCAM 01017 and may be used in transformation of organic remains of natural origin.

EFFECT: improved productivity of agroecosystem.

3 ex

FIELD: process engineering.

SUBSTANCE: set of invention relates to water treatment. Proposed method comprises immobilisation of biomass bearing oil-oxidisers taken in effective amount into organic hydrophobic peat-based sorbent and drying it. Note here that said organic hydrophobic sorbent is prepared by low-temperature pyrolysis in vacuum of peat minced to 0.2-0.5 mm size at 210-250°C for 60-90 minutes. Said immobilisation is performed by adding sorbent into biomass suspension during its fermentation at retardation of oil-oxidising microorganisms growth. Note here that mass of added sorbent is 6-10 times larger than that of microorganisms contained in biomass suspension at the moment of sorbent application. Said oil-oxidising microorganisms represent yeast culture Candida maltosa All-Russian collection of industrial microorganisms Y-3446 or culture of bacteria Dietzia maris All-Russian collection of industrial microorganisms Ac-1824. Besides, this invention covers biosorbant for water treatment thus produced.

EFFECT: faster preparation, higher absorption capacity and efficiency.

2 cl, 4 ex

FIELD: biotechnologies.

SUBSTANCE: invention may be used to produce a new biopreparation for cleaning of water, soil, industrial drains from pesticides resistant to decomposition and selected from chlorophenoxyacetic acids, such as 2,4-dichlorophenoxyacetic acid (2,4-D), trichlorophenoxyacetic acid (2,4,5-T), chlorophenoxyacetic acid (CPAA), phenoxyacetic acid (PAA), - 2,4-dichlorophenoxy-α-propionic acid, 2-methyl-4-chlorophenoxy-α-propionic acid, 2,4,5-trichlorophenoxy-α-propionic acid, 2,4-dichlorophenoxy-α-butyric acid, methyl-[1-(butylamino) carbonyl]-1H-benzimidazol-2-ylcarbamate, 2,4-dichlorophenol, imidoclapride, hexachlorohexane, and also phenol. The biopreparation is an association of strains of bacteria Pseudomonas putida VKPM V-10997, Bacillus cubtilis VKPM V-10999 and Rhodococcus erythropolis VKPM - As-1882 at the weight ratio of (1-2):(1-2):1. At the same the biopreparation, as a rule, contains additionally a sorbent, organic, mineral and stimulating additives and has activity that stimulates growth of plants and fungicide properties. The produced biopreparation is introduced as an aqueous solution by means of sprinkling into polluted soil or industrial drain in efficient amount.

EFFECT: invention makes it possible to improve cleaning of water, soil, industrial drains from pesticides resistant to decomposition.

21 tbl, 29 ex

FIELD: biotechnologies.

SUBSTANCE: strain of bacterial Pseudomonas panipatensis VKPM V-10593 is proposed, which is capable of quick recycling of oil and oil products, in particular, diesel fuel. Strain may be used to clean soil and water reservoirs contaminated with oil and oil products, in a wide range of temperatures from +8 to +30°C.

EFFECT: improved properties of a strain.

2 tbl, 5 ex

FIELD: process engineering.

SUBSTANCE: invention relates to biotechnology and may be used for wasteless cleaning of water and solid surfaces from oil and oil product outflow. Proposed material comprises outer layer of polyester fibers and mid layer of polypropylene fibers comprising incorporated phosphorus-containing cationites and/or nitrogen-containing anionites, cell walls of hydrophytes and immobilised cells of oil destruction bacteria. Proposed biohybrid material features sorption capacity of, at least, 25 g/g of sorbent and degree of bacteria cell load of, at least, 150 mg/m2 and may be used may times: at least, 5 regeneration cycles with extraction of, at least, 90% of sorbate in first cycle.

EFFECT: wasteless cleaning of water and solid surfaces from oil and oil product outflow.

2 ex

FIELD: chemistry.

SUBSTANCE: invention relates to biotechnology. The Bacillus atropheus IPNG - ELA-2 bacteria strain, having recycling capacity with respect to oil and oil products, is deposited in the Russian National Collection of Industrial Microorganisms (VKPM) under registration number VKPM V-10592 and can be used to clean water and soil from oil.

EFFECT: invention enables to cut the duration of recycling oil and oil products.

4 tbl, 6 ex

FIELD: environment protection.

SUBSTANCE: method involves mechanical removal of excessive contaminants from surface and reducing toxicity of contaminated soil; sowing green manure crops and perennial grasses; preliminarily removing taking soil samples from contaminated surface and separating native contaminant destructors therefrom; after mechanical removal of contaminant from soil surface, providing sequential treatment of plot soil with water-soluble humates and at least double treatment with native contaminant destructors; mellowing soil; applying lime with mineral fertilizers such as saltpeter, double superphosphate and calcium chloride. Green manure crops are sown after application of fertilizers. Method further involves grinding green manure crops, plowing into soil and covering with soil by means of covering roll.

EFFECT: increased efficiency, reliable ecologically clean restoration of soil after contamination thereof.

Up!