Strain rhodococcus erythropolis, used for decomposition of oil

FIELD: biotechnologies.

SUBSTANCE: strain Rhodococcus erythropolis 1-KP, extracted from podzol soil contaminated with oil from the territory of the Kolsky peninsula, having high speed of oil utilisation, is deposited in the Departmental Collection of Beneficial Microorganisms of Agricultural Purpose of the Russian Academy of Agricultural Sciences (RCAM) (GNU VNIISHM) under the number RCAM01142 and may be used for treatment of contaminated soils from oil.

EFFECT: improved quality of soil cleaning from oil.

3 tbl

 

The invention relates to the microbiological industry and relates to a new culture of microorganisms, destroying the oil, and which can be used to clean contaminated soils.

A known strain of Rhodococcus erythropolis for the decomposition of oil and oil products (RF Patent 2257409, MKI C12N 1/20, VC 1/10, publ. 2004). The strain deposited as Rhodococcus erythropolis PMBC AC-1668. The strain isolated from a sample of the oily soil of the Republic of Bashkortostan, grows on many natural and synthetic environments and has a relatively high rate of utilization of oil and petroleum products.

It should be noted that a significant drawback of the considered strain is the fact that this microorganism has the ability to biological oxidation of heavy and aromatic hydrocarbons and other harmful chemical compounds, such as alcohols, aldehydes, technical oils, fats and other

Known consortium of strains of hydrocarbon-oxidizing microorganisms Rhodococcus erythropolis IT BKM Ac-1769 and Pseudomonas fluorescens BKM-6847 (RF Patent 2428471, C12N 1/26, C02F 3/34, VC 1/10, publ. 2010), included embioptera "Centrum-MMS", designed for the removal of pollutants hydrocarbon nature, primarily from oil and fuel substances and allows to increase the efficiency of cleaning of the environment from oil and oil products./p>

However, the disadvantage of this invention is that the cultivation of microorganisms of different taxonomic and species, as well as differing in growth rate, substrate specificity, temperature optimum growth causes them to be more competitive and reduces their survival, and as a consequence complicates and makes the process of growing more financially and emotionally.

The objective of the invention is the expansion of the means and obtaining a new strain, characterized by a high rate of recycling oil.

To achieve the objectives proposed a strain of Rhodococcus erythropolis 1-KP. The strain is deposited in the Departmental collection of useful microorganisms for agricultural purposes RAAS (RCAM) number RCAM01142.

The strain Rhodococcus erythropolis 1-KP isolated from oil-contaminated podzolic soils of the Kola Peninsula.

The selection of the strain was carried out by culturing the original sample is contaminated soil in liquid mineral medium with the addition of hydrocarbons with subsequent sieving on mastopathy agar (MPA) to obtain isolated colonies.

Identification of the strain was carried out on the basis of a study of its cultural-morphological and physiological-biochemical characteristics in accordance with the description given in about the radiotele bacteria Bergey (Bergey's. Manual of Systematic Bacteriology. 1986. 8thed V.2. Baltimore-Londone, William & Wilkins), as well as on the basis of a study of the sequence of 16S rRNA genes.

Cultivation in laboratory conditions was carried out on nutrient medium of the following composition:

1) Wednesday MPA (sucrose - 5.0 g/l; glucose - 5.0 g/l; MPA - 50 g/l; agar-agar - 8.0 g/l), pH of 7.3-7.5 to sterilization.

2) Mineral medium with C14-C17for practical conservation of cultural values:

(K2HPO4- 0.5 g/l; KH2PO4- 0.5 g/l; MgSO40.3 g/l; NH4H2PO4- 1.0 g/l; peptone - 2.0 g/l; agar-agar - 15,0; after editing environment in a test tube prior to sterilization naslovom ~0.8 ml fractions of liquid hydrocarbons From14-C17), pH of 7.1 to 7.2 before sterilization.

Growing conditions: medium MPA 96 hours at 28°C in mineral medium with hydrocarbons 96-120 hours at 28°C.

Storage of strain produced by periodic subculture to fresh medium 1 time per 3 months.

The morphological characteristics of the strain is characterized as gram-positive Bacillus size(0,6-0,8)×(2,3-2,5) μm, does not form spores, motionless.

This strain is characterized by the following cultural-morphological features. Colonies on mesopatamia agar under cultivation at T=28°C shiny, convex, smooth, beige mucous with smooth edge. The size of the colonies not p is Evesham 5.0 mm in three days after sowing. Is an aerobe. Grows in the temperature range from 4 to 35°C. the Optimum temperature of 28°C. Grows at a pH of from 3.5 to 9.0, the optimal value of pH of 6.9.

The strain Rhodococcus erythropolis 1-KP is characterized by the following biochemical characteristics. Assimilates maltose, mannose, fructose, sucrose, glucose, hydrocarbons, ethyl alcohol, glycerin. Consumes acetic, propionic, butyric, citric, salicylic acid. Assimilates ammonia nitrogen, nitrate nitrogen. Does not require supplementation of growth factors and vitamins in a nutrient medium for cultivation. Gelatino not thins, hydrolyzes starch is not. Kislotoobrazovanie during growth on sugars and hydrocarbons weak. Indole and hydrogen sulfide is not formed. Catalanopolonesa. Resistant to cefazedone, polymyxin, cephalexin.

The practical application of this strain is due to its physiological and biochemical characteristics. The strain is designed to eliminate the effects of soil contamination with oil.

Below are examples of biological oxidation of oil by a strain of Rhodococcus erythropolis 1-KP.

Prepared for the experiments of garden soil was put up to 1.5 kg in laboratory battery glasses with a capacity of 5 litres each. Soil contamination with oil was carried out directly in the glasses. As oil pollutants were selected two samples of oil, the quality of the public whose composition is given in Table 1. Linkage of oil pollutants in the ground made by the gravimetric method and was ~30 g, or ~2.0 wt% on the soil. Glasses with oil-contaminated soil was maintained in laboratory conditions with periodic loosening. This contributed to a more uniform distribution of oil pollutants in the soil and, if possible, the evaporation of its light fractions.

In Table. 1 presents the results of chemical bitumen analysis of an average sample of soil contaminated by oil samples, wt.%. Oil 1 - naphthenic-aromatic heavy oil field "Russian". Oil 2 - methane-naphthenic light oil field "Western Tabuk".

Experimental evaluation of the ability of microorganisms to biodegradeable oil was carried out in laboratory conditions. In the prepared soil samples, pre-packaged in 1.5 kg in laboratory battery glasses with a capacity of 5 liters each, and contaminated oil and diesel fuel have made the culture of microorganisms with a titer of 105-108cells/ml Uterine culture bacteria Rhodococcus erythropolis 1-KP were grown on a shaker (200 rpm) at 28°C for 48-72 hours in flasks with 100 ml of one of the media of the following composition, wt.%:

1. Peptone - 1%

K2HPO4- 0,15%

MgSO4- 0,15%

Glycerol - 1%

Tap water - the rest is up to 100%

pH before sterilization 7,6;

2. Meat is atony broth with glucose (0.5%wt.) and sucrose (0.5 weight %).

pH before sterilization 7,6.

Experimental evaluation of the ability of microorganisms to biodegradeable oil was carried out in laboratory conditions. In the prepared soil samples, pre-packaged in 1.5 kg in laboratory battery glasses with a capacity of 5 liters each, and contaminated oil was made by culture of microorganisms with a titer of 105-108cells/ml Uterine culture bacteria Rhodococcus erythropolis 1-KP were grown on a shaker (200 rpm) at 28°C for 48-72 hours in flasks with 100 ml of one of the media of the following composition, wt.%:

1. Peptone - 1%

K2HPO4- 0,15%

MgSO4- 0,15%

Glycerol - 1%

Tap water - the rest is up to 100%

pH before sterilization 7,6;

2. Mastopathy broth with glucose (0.5%wt.) and sucrose (0.5 weight %).

pH before sterilization 7,6.

The experiments were conducted at room temperature (average 18-20°C) for 91 days with periodic mixing and hydration as drying of the soil. Hydration was carried out in sterile water; approximately every 3 weeks hydration was carried out in mineral medium containing K, R, N, and having a pH before sterilization of 7.1 to 7.2. The soil in the control experiments were moistened only with sterile water.

Accounting for the number of living cells of microorganisms in the experiment was conducted in the conventional method of limiting time is the introduction to elective environments.

Monitoring of ongoing processes of purification of oil-contaminated soil was carried out according to the following parameters:

- oil content, mg/kg

- the content and qualitative composition of the chloroform extract.

The frequency of sampling soil on these kinds of analyses was typically 2 weeks; the analysis was carried out according to GOST 17.1.4.01-80 and RD 52.18.575-96.

Table 2 shows the results of the monitoring of the content of petroleum products (NP), wt.% from the initial content in the soil when cleaning the strain of impurities, the composition of which is given in Table. 1. Given in wt.% relative to their original content in oil-contaminated soil.

Table 2
Oil content (NP) in contaminated soil in the cleaning process by a strain of Rhodococcus erythropolis 1-KP
PollutantThe content of the NP, wt.% from the initial content in the soil
The duration of treatment, weeks
0246813
Oil
Oil 10,0-13,7-32,1-47,2-62,4-62,5
Oil 20,0-16,9-20,1-42,5-65,0-71,0

According to the table, for 13 weeks duration of experience of oil content in the contaminated soil samples is reduced by 62.5-71,0% of the original content. The decrease in the content of oil products in samples contaminated with oil, there is 2 weeks from the beginning of the experiments.

In Table 3 shows the content and qualitative composition of HBA oil-contaminated soil samples Table 1 through 91 days from the beginning of the experiments cleanup strain of Rhodococcus erythropolis 1-KP.

Data quality HBA - (chloroform of bitumen-α) samples of contaminated soil prior to cleaning, is presented in table 1, in comparison with experimental samples, are presented in Table 3, allow to analyze the nature of changes in group and hydrocarbon composition chloroform bat is Aida α in the process of biological oxidation strain of Rhodococcus erythropolis 1-KP.

According to the results of the analysis, the content of HBA in experimental models of oil-contaminated soil varies in the range of 1.33-2.43 per cent during the duration of the experiments 91 days. Has changed dramatically and qualitative composition of HBA samples of soil, compared with doopity composition of HBA. After 91 days after the start of the experiments on the utilization of oil-contaminated soils has decreased the most accessible for microorganisms its fractions. The most available for the oxidation process in the composition of oil pollution is the fraction oils. The oil content decreased to 38,55-42,25% Rel.

Based on the data presented above, it follows that the strain Rhodococcus erythropolis 1-KP RCAM01142 characterized by high efficiency in the processes of recycling of oil, and as a consequence can be used for biological treatment of soils from these pollutants.

It should also be noted that the strain Rhodococcus erythropolis 1-KP oxidizing microorganism can be used in the processes of biological oxidation of petroleum products such as diesel fuel. The basis for this conclusion is the fact that the composition of diesel fuel and other petroleum products, as well as the composition of oils are different classes of hydrocarbons, which are subject to biological degradation by microorganisms.

The strain Rhodococcus erythropolis deposited in the Agency the military Collection of Useful Microorganisms for Agricultural Purposes RAAS (RCAM) (GNUS ARRIAM) under registration number RCAM 01142 and used for the decomposition of the oil.



 

Same patents:

FIELD: biotechnologies.

SUBSTANCE: strain pseudomonas citronellolis 48-U, having high speed of oil and diesel fuel recycling, is deposited in the Departmental Collection of Beneficial Microorganisms of Agricultural Purpose of the Russian Academy of Agricultural Sciences (RCAM) (GNU VIISHM) under the registration number RCAM 01441 and may be used for treatment of contaminated soils from oil and diesel fuel.

EFFECT: improved quality of soil cleaning from oil and diesel fuel.

3 tbl

FIELD: biotechnologies.

SUBSTANCE: strain Rhodococcus fascians 4-G, extracted from soil contaminated with black oil and sampled from the territory of a boiler plant located in the settlement Gorelovo, Leningrad region, is deposited in the Departmental Collection of Beneficial Microorganisms of Agricultural Purpose of the Russian Academy of Agricultural Sciences (RCAM) (GNU VNIISHM) under the number RCAM01140 and may be used for treatment of contaminated soils from oil.

EFFECT: invention makes it possible to increase quality of soil treatment from oil.

3 tbl

FIELD: biotechnologies.

SUBSTANCE: strain Pseudomonas aeruginosa RCAM01139 is proposed for decomposition of oil and diesel fuel.

EFFECT: strain is characterised by high efficiency in processes of oil and diesel fuel utilisation, which are the only sources of carbon and energy.

3 tbl

FIELD: biotechnologies.

SUBSTANCE: strain Micrococcus luteus PBT-1, having high catalase activity and aiding increasing productivity of the agroecosystem, participating in processes of transformation of organic remains of natural origin, is deposited in the Departmental Collection of Useful Microorganisms of Agricultural Purpose of the Russian Agricultural Academy (RCAM) (GNU VNIISHM) under the registration number RCAM 01016 and may be used in transformation of organic remains of natural origin.

EFFECT: invention makes it possible to increase productivity of an agroecosystem.

3 ex

FIELD: biotechnologies.

SUBSTANCE: strain Penicillium sp. PBT-2 extracted from samples of humic pod mixed with cereal straw and having polyfunctional properties and promoting increased productivity of agroecosystem, participating in processes of transformation of organic remains of natural origin, is deposited in the Departmental Collection of Beneficial Microorganisms of Agricultural Purpose of the Russian Academy of Agricultural Sciences (RCAM) (GNU VNIISHM) under the registration number RCAM 01017 and may be used in transformation of organic remains of natural origin.

EFFECT: improved productivity of agroecosystem.

3 ex

FIELD: biotechnologies.

SUBSTANCE: strain of nodule bacteria Bradyrhizobium japonicum 859 (VNIISHM No.24117) is proposed, used as a facility to produce a fertiliser for soya. When soya seeds are treated with the fertiliser produced on the basis of the specified strain Bradyrhizobium japonicum, the index of germination increases by more than 8%, and crop capacity increases by 17-30%.

EFFECT: higher germination of plants, improved crop capacity.

4 tbl

FIELD: process engineering.

SUBSTANCE: set of invention relates to water treatment. Proposed method comprises immobilisation of biomass bearing oil-oxidisers taken in effective amount into organic hydrophobic peat-based sorbent and drying it. Note here that said organic hydrophobic sorbent is prepared by low-temperature pyrolysis in vacuum of peat minced to 0.2-0.5 mm size at 210-250°C for 60-90 minutes. Said immobilisation is performed by adding sorbent into biomass suspension during its fermentation at retardation of oil-oxidising microorganisms growth. Note here that mass of added sorbent is 6-10 times larger than that of microorganisms contained in biomass suspension at the moment of sorbent application. Said oil-oxidising microorganisms represent yeast culture Candida maltosa All-Russian collection of industrial microorganisms Y-3446 or culture of bacteria Dietzia maris All-Russian collection of industrial microorganisms Ac-1824. Besides, this invention covers biosorbant for water treatment thus produced.

EFFECT: faster preparation, higher absorption capacity and efficiency.

2 cl, 4 ex

FIELD: process engineering.

SUBSTANCE: set of invention relates to water treatment. Proposed method comprises immobilisation of biomass bearing oil-oxidisers taken in effective amount into organic hydrophobic peat-based sorbent and drying it. Note here that said organic hydrophobic sorbent is prepared by low-temperature pyrolysis in vacuum of peat minced to 0.2-0.5 mm size at 210-250°C for 60-90 minutes. Said immobilisation is performed by adding sorbent into biomass suspension during its fermentation at retardation of oil-oxidising microorganisms growth. Note here that mass of added sorbent is 6-10 times larger than that of microorganisms contained in biomass suspension at the moment of sorbent application. Said oil-oxidising microorganisms represent yeast culture Candida maltosa All-Russian collection of industrial microorganisms Y-3446 or culture of bacteria Dietzia maris All-Russian collection of industrial microorganisms Ac-1824. Besides, this invention covers biosorbant for water treatment thus produced.

EFFECT: faster preparation, higher absorption capacity and efficiency.

2 cl, 4 ex

FIELD: biotechnology.

SUBSTANCE: basic fermentation medium is prepared on the basis of fermentative lysate of starch in an amount of 8-15% for glucose, and additional fermentation medium containing the components of basic fermentation medium, 2-3.5 times concentrated. They are sterilised. Biosynthesis of L-lysine is carried out using strain-producer Corynebacterium glutamicum of All-Russia classification of microorganisms Ac-2577D (BIGOR 55). The fermentor is loaded with the basic nutrient medium in amount of 1/3 of its volume. Then inoculation of the basic fermentation nutrient medium is carried out with 10-20% inoculum grown in two stages. Biosynthesis process is carried out with continuous feeding starting from 8-16 hours of cultivation. At that the inhibitory concentration of reducing substances in the culture fluid of 4-8% is maintained. After filling the device to the maximum volume after 60-66 hours of cultivation and then every 6-12 hours for the rest part of biosynthesis process a part of the culture fluid in amount of 10-15% of the maximum volume is pumped into the device for final fermentation. In this device, the biosynthesis process is continued for 6-12 hours under the same conditions as in the main device but without feeding the additional nutrient medium. Then L-lysine is isolated.

EFFECT: invention enables to carry out the biosynthesis process for a long time without reducing the rate of synthesis of L-lysine, and provides obtaining of the culture fluid with high concentration of L-lysine prior to the isolation.

4 ex

FIELD: biotechnology.

SUBSTANCE: method provides sampling of the material under study. Inoculation of the material under study on nutrient medium sabouraud followed by incubation on this medium at 37°C for 24 hours. A quantitative assessment of growth of yeast-like fungi of the genus Candida, and with a value exceeding 10*1 CFU/swab the candidiasis of upper respiratory tract is diagnosed. The obtained colonies are examined under the microscope and the isolated colonies of yeast-like fungi of the genus Candida are separated from these colonies. The isolated colonies of yeast-like fungi of the genus Candida are suspended in five test tubes containing liquid medium sabouraud with the phenol red indicator, where the discs with carbohydrates are added, and in the first test tube - a disc containing maltose, in the second - sucrose, the third - lactose, the fourth-galactose, the fifth - trehalose, and are incubated at 37°C for 24 hours, followed by assessment of colour change of the indicator. The colour change of the indicator to yellow is taken as one, lack of colour change is taken as zero, the sum of the values obtained in assessment of the indicator colour in five test tubes is calculated. If the value of the sum is 0 the candidiasis of upper respiratory tract caused by Candida kruzei is diagnosed, if it is 1 - the candidiasis of the upper respiratory tract caused by Candida glabrata is diagnosed, if it is 3 - the candidiasis of the upper respiratory tract caused by Candida albicans is diagnosed, if it is 4 - the candidiasis of the upper respiratory tract caused by Candida tropicalis is diagnosed, if it is 5 - the rest.

EFFECT: invention enables to identify several types of yeast-like fungi of the genus Candida, causing candidiasis, and enables to take into account the quantitative growth of yeast-like fungi of the genus Candida, according to which the presence of candidiasis is judged.

6 ex

FIELD: biotechnologies.

SUBSTANCE: strain pseudomonas citronellolis 48-U, having high speed of oil and diesel fuel recycling, is deposited in the Departmental Collection of Beneficial Microorganisms of Agricultural Purpose of the Russian Academy of Agricultural Sciences (RCAM) (GNU VIISHM) under the registration number RCAM 01441 and may be used for treatment of contaminated soils from oil and diesel fuel.

EFFECT: improved quality of soil cleaning from oil and diesel fuel.

3 tbl

FIELD: biotechnologies.

SUBSTANCE: strain Rhodococcus fascians 4-G, extracted from soil contaminated with black oil and sampled from the territory of a boiler plant located in the settlement Gorelovo, Leningrad region, is deposited in the Departmental Collection of Beneficial Microorganisms of Agricultural Purpose of the Russian Academy of Agricultural Sciences (RCAM) (GNU VNIISHM) under the number RCAM01140 and may be used for treatment of contaminated soils from oil.

EFFECT: invention makes it possible to increase quality of soil treatment from oil.

3 tbl

FIELD: biotechnologies.

SUBSTANCE: strain Pseudomonas aeruginosa RCAM01139 is proposed for decomposition of oil and diesel fuel.

EFFECT: strain is characterised by high efficiency in processes of oil and diesel fuel utilisation, which are the only sources of carbon and energy.

3 tbl

FIELD: biotechnologies.

SUBSTANCE: strain Micrococcus luteus PBT-1, having high catalase activity and aiding increasing productivity of the agroecosystem, participating in processes of transformation of organic remains of natural origin, is deposited in the Departmental Collection of Useful Microorganisms of Agricultural Purpose of the Russian Agricultural Academy (RCAM) (GNU VNIISHM) under the registration number RCAM 01016 and may be used in transformation of organic remains of natural origin.

EFFECT: invention makes it possible to increase productivity of an agroecosystem.

3 ex

FIELD: biotechnologies.

SUBSTANCE: strain Penicillium sp. PBT-2 extracted from samples of humic pod mixed with cereal straw and having polyfunctional properties and promoting increased productivity of agroecosystem, participating in processes of transformation of organic remains of natural origin, is deposited in the Departmental Collection of Beneficial Microorganisms of Agricultural Purpose of the Russian Academy of Agricultural Sciences (RCAM) (GNU VNIISHM) under the registration number RCAM 01017 and may be used in transformation of organic remains of natural origin.

EFFECT: improved productivity of agroecosystem.

3 ex

FIELD: process engineering.

SUBSTANCE: set of invention relates to water treatment. Proposed method comprises immobilisation of biomass bearing oil-oxidisers taken in effective amount into organic hydrophobic peat-based sorbent and drying it. Note here that said organic hydrophobic sorbent is prepared by low-temperature pyrolysis in vacuum of peat minced to 0.2-0.5 mm size at 210-250°C for 60-90 minutes. Said immobilisation is performed by adding sorbent into biomass suspension during its fermentation at retardation of oil-oxidising microorganisms growth. Note here that mass of added sorbent is 6-10 times larger than that of microorganisms contained in biomass suspension at the moment of sorbent application. Said oil-oxidising microorganisms represent yeast culture Candida maltosa All-Russian collection of industrial microorganisms Y-3446 or culture of bacteria Dietzia maris All-Russian collection of industrial microorganisms Ac-1824. Besides, this invention covers biosorbant for water treatment thus produced.

EFFECT: faster preparation, higher absorption capacity and efficiency.

2 cl, 4 ex

FIELD: biotechnologies.

SUBSTANCE: invention may be used to produce a new biopreparation for cleaning of water, soil, industrial drains from pesticides resistant to decomposition and selected from chlorophenoxyacetic acids, such as 2,4-dichlorophenoxyacetic acid (2,4-D), trichlorophenoxyacetic acid (2,4,5-T), chlorophenoxyacetic acid (CPAA), phenoxyacetic acid (PAA), - 2,4-dichlorophenoxy-α-propionic acid, 2-methyl-4-chlorophenoxy-α-propionic acid, 2,4,5-trichlorophenoxy-α-propionic acid, 2,4-dichlorophenoxy-α-butyric acid, methyl-[1-(butylamino) carbonyl]-1H-benzimidazol-2-ylcarbamate, 2,4-dichlorophenol, imidoclapride, hexachlorohexane, and also phenol. The biopreparation is an association of strains of bacteria Pseudomonas putida VKPM V-10997, Bacillus cubtilis VKPM V-10999 and Rhodococcus erythropolis VKPM - As-1882 at the weight ratio of (1-2):(1-2):1. At the same the biopreparation, as a rule, contains additionally a sorbent, organic, mineral and stimulating additives and has activity that stimulates growth of plants and fungicide properties. The produced biopreparation is introduced as an aqueous solution by means of sprinkling into polluted soil or industrial drain in efficient amount.

EFFECT: invention makes it possible to improve cleaning of water, soil, industrial drains from pesticides resistant to decomposition.

21 tbl, 29 ex

FIELD: chemistry.

SUBSTANCE: invention relates to biotechnology. The Bacillus atropheus IPNG - ELA-2 bacteria strain, having recycling capacity with respect to oil and oil products, is deposited in the Russian National Collection of Industrial Microorganisms (VKPM) under registration number VKPM V-10592 and can be used to clean water and soil from oil.

EFFECT: invention enables to cut the duration of recycling oil and oil products.

4 tbl, 6 ex

FIELD: process engineering.

SUBSTANCE: invention relates to biological treatment of industrial and domestic effluents. Proposed method comprises feeding effluents into reservoir with further transfer of treated effluents in second reservoir, forcing them into outlet, and purification. Active aeration of said effluents is carried out at air flow rate of 20-30 litres a minute in first reservoir. The latter features diameter-to-height ratio of 0.2-0.3. Conditionally pure waters (pools, bathrooms, showers) in notable volumes are directed in second reservoir for mixing with purified effluents from first reservoir with lower content of dissolved air and sludge suspension sufficient for purification of effluents from pools, bathrooms, and showers. Proposed activation plant comprises reservoir with effluents feed line including air discharge line and sludge discharge pump, second reservoir with purified water discharge pump, and discharge opening. First reservoir is filled so that diameter-to-height ratio, for reservoir filled part, makes 0.2-0.3 to provided for boiling bed in treatment. Second reservoir is communicated with first one via inclined line to accommodate conditionally pure waters for their afterpurification on mixing with treated effluents from first reservoir.

EFFECT: higher efficiency of treatment, reliable operation, lower costs.

2 cl, 1 dwg

FIELD: chemistry.

SUBSTANCE: group of inventions relates to biotechnology. Disclosed is a method for biological decontamination of liquid hydrocarbon-containing wastes which are formed when cleaning natural gas and gas pipelines. A biopreparation of hydrocarbon-oxidising microorganisms is added to liquid hydrocarbon-containing wastes in amount of 1 kg of biopreparation per 10 kg of hydrocarbons. Water is simultaneously added to the working vessel to obtain a working suspension with volume ratio of wastes to water ranging from 1:4 to 1:50, depending on the initial concentration of hydrocarbons. Macroelements, trace elements and, optionally, an acidity regulator are then added. Decontamination is carried out for 12-15 hours while constantly stirring and feeding air. Temperature is kept in the range from 28°C to 36°C and pH is kept in the range 4 to 7. The decontamination product is then discharged from the working vessel, leaving not less than 25% of the volume of the working suspension. The whole decontamination cycle is repeated. The second and subsequent decontaminations are carried out without adding the biopreparation. Also disclosed is apparatus for biological decontamination of liquid hydrocarbon-containing wastes which are formed when cleaning natural gas and gas pipelines. The apparatus has a working vessel with process pipes, a mixer and a supply vessel mounted on top of the working vessel and linked to the working vessel by a connecting pipe. The mixer is fitted with an electric drive and is mounted with possibility of turning about a vertical axis. The working vessel is fitted with a level gauge, a loading bin for the biopreparation, a vessel for trace elements and macroelements, a vessel for the acidity regulator and a built-in heat-exchanger. The connecting pipe is fitted with a stop valve and a pipe for feeding water.

EFFECT: shorter duration and higher efficiency of the decontamination process, wider field of using the method and the apparatus.

4 cl, 2 dwg, 1 tbl

FIELD: biotechnologies.

SUBSTANCE: strain pseudomonas citronellolis 48-U, having high speed of oil and diesel fuel recycling, is deposited in the Departmental Collection of Beneficial Microorganisms of Agricultural Purpose of the Russian Academy of Agricultural Sciences (RCAM) (GNU VIISHM) under the registration number RCAM 01441 and may be used for treatment of contaminated soils from oil and diesel fuel.

EFFECT: improved quality of soil cleaning from oil and diesel fuel.

3 tbl

Up!