Method of moving opaque microobjects

FIELD: physics.

SUBSTANCE: according to the method of moving a group of opaque microobjects, a light beam with closed regions of zero intensity is formed from multiple beams. First, three coaxial, zero-order Bessel beams with different propagation constants are formed, thereby forming a stable beam in form of a circular spot. These beams are then arranged in space so as to form one or more closed regions for capturing and moving opaque microparticles.

EFFECT: high efficiency owing to automation of the process.

11 dwg


The invention relates to the field of optical microscopy and optical micromanipulation.

One of the applications of devices, commonly referred to as "optical tweezers" (optical tweezer), - moving and Assembly of elements of micromechanics specially shaped light beam. The light beam due to the gradient force in the beam of light moves a single or group of micro-objects of a special form (US patent 7622710, IPC G01B 21/06, publ. 24.11.2009 g, US patent 6995351, IPC G01N 30/00, publ. 11.08.2005,, US patent 7678222, IPC G03H 1/00, publ. 28.12.2006,).

The disadvantage of all these methods of rotation of the vortex is the nature of light beams to capture opaque micro-objects. This causes unnecessary when moving the rotation of the micro-object.

The closest technical solution, selected as a prototype, is a way to move the micro-objects, which consists in the formation of the vortex light beam (US patent 6995351, IPC G01N 30/00, publ. 11.08.2005,).

The main disadvantage of this method is the presence of a torque micro-object forces, which complicates the Assembly of micromechanical systems this method and the automated build process.

The basis of the invention the task is to increase productivity through automation of the process.

This task is achieved by the fact that the way to move groups opaque mi is noobject, which consists in forming a light beam with closed regions of zero intensity, according to the invention the light beam to form multiple beams, first use three coaxial beam of the zero order Bessel with different propagation constants, forming a stable beam in the form of round spots, then these bundles are placed in the space so as to form one or more closed regions to capture and move the opaque particles.

The proposed solution differs from prototype that uses the superposition of multiple Bessel beams of the 0-th order, so you can form a group of three-dimensional optical traps for opaque micro-objects without limitation of distance between traps with and without polar phase gradient in each trap. Thus, removes the disadvantage of the group of traps in the form of a conventional vortex beams that are impossible to bring to a distance less than their own diameter, and which in addition in the capture of the micro-object begin to rotate.

These differences allow to draw a conclusion on the conformity of the proposed technical solutions to the criterion "novelty". The features distinguishing the claimed technical solution to the prototype, not identified in other technical solutions in the study of this and related areas of technology and thus the consequently, ensure proposed solutions meet the criterion of "significant differences".

Figure 1 shows the optical layout of the device that implements the method.

The device consists of a solid state laser with a wavelength of 532 nm and a maximum average power of 500 mW, a rotary mirror 2 and mirror lighting system, the diffractive optical element 4, the cell with micro-objects 5, depicting a micro 6, a rotary mirror 7 of the imaging system, the CCD camera 8, the control computer 9, the illuminator lamp 10, condenser illuminator 11, focusing microobjective 12.

Light trap for opaque micro-objects in English-language scientific articles usually called optical "bootle" (optical "bottle") or light "bootle" light "bottle"). Invited to create a group of optical "bottles" to use the superposition of beams (modes) Bessel having different parallel axis distribution.

The method is as follows.

On the diffraction optical element is sent to a beam of coherent light from a laser. After the diffraction element is formed by a closed three coordinates of the dual optical trap 11.

As can be seen from 11, dual light trap begins to form at a distance of 775 mm from the element and closes on the races is the being 975 mm When focusing indicated in figure 1 by a micro longitudinal length of such traps is only 40 microns.

For group formation optical "bottles" consider the superposition of N spatially separated fashion Bessel different orders with different numbers of roots of the Bessel functions, i.e. different values of m used in the calculation of the phase function of the diffraction grating forming the beam Bessel n-th order


where αm=kρm,ρm=1-(σ0+mλz0)2, σ0=cosθ, n is the order of the Bessel functions, m is the number of the root of the Bessel functions, k is the wave number, θ is the average angle of inclination of plane waves of the spatial spectrum for the given field, x, y - Cartesian coordinates.

For calculating, key writing, the superposition of N spatially separated fashion Bessel used the following formula:


with complex coefficients Ckfor each individual fashion.

Although for the formation of a single optical trap for opaque micro-objects, there are simpler ways, such as using composite axicon, it is possible to form a trap by the method described above, to demonstrate the versatility of the proposed approach.

These 27 modes are according to the scheme presented in figure 2.

In this and subsequent diagrams for the unit undertook the minimum size of the diffraction spots generated by a given element at a distance of z 0from the input plane at a wavelength of λ=532 nm.

In each of the positions in the diagram is a superposition of three modes Bessel 0-th order with the same values of z0=800 mm, but with different numbers of roots of Bessel functions m=8, 9, 10. For all modes except those in the Central position of the coefficients Ck=1; for mod in the Central position Ck=1·e.

Figure 3 presents the amplitude and the phase of the diffraction optical element designed to form a single trap.

Amplitude-phase distribution of an element forming such a superposition is presented in figure 4.

If you ignore the amplitude component, and to consider only the phase, the distances 780-880 mm when the diameter of the element 6 mm and the diameter of the illuminating beam 4.4 mm obtained the following distribution of the light field is presented in figure 4. Distribution obtained through equal distances from each other (20 mm).

As can be seen from figure 4, really formed the classical optical bottle, closed on all three coordinates. This relatively large size of the trap along the axis of propagation (100 mm) applies only to the formation of her in free space. When focusing this beam micro 90× its length is reduced to 20 μm. The efficiency of the pump is the eye is about 67%. Let's consider some more complex optical configurations "bottles". We will consistently increase their number.

For forming a double light "bottle" will require the superposition of more mod Bessel. In this case, 45 mod Bessel located at the scheme shown in figure 5.

In each of the positions in the diagram is the superposition of the same three modes Bessel 0-th order with the same values of z0=800 mm, but with different numbers of roots of Bessel functions m=8, 9, 10 (as for a single bottle). For all modes the coefficients Ckwere real numbers and equal to:

for mod, located at the points with coordinates[1; -1], [1; 1], [-1; -1]; [-1; 1] Ck=2.75;

for modes located at the point with coordinates [0; 0] Ck=2.25;

- for other modes of Ck=2.0;

Amplitude-phase distribution of an element forming such a superposition presented on Fig.6.

If you ignore the amplitude component, and to consider only the phase, the distances 780-880 mm when the diameter of the element 6 mm and the diameter of the illuminating beam 4.4 mm obtained the following distribution of the light field (Fig.7). Distribution obtained through equal distances from each other (20 mm).

The efficiency of the traps, compared with single, slightly decreased and amounted to about 45%.

For fo the formation of three contiguous traps need 66 mod Bessel be placed under the scheme, presented at Fig.

In each of the positions in the diagram is the superposition of the same three modes Bessel 0-th order with the same values of z0=800 mm, but with different numbers of roots of Bessel functions m=8, 9, 10 (as for a single bottle). For all modes the coefficients Ckwere real numbers and equal to:

for mod, located at the points with coordinates[5; -1], [5; 1], [-5; -1]; [-5; 1] Ck=1.75;

for modes located at the point with coordinates[3; -1]; [3; 1], [2; 0], [-2; -0]; [-3; -1], [-3; 1] Ck=2.25;

for modes located at the point with coordinates[6; 0]; [-6; 0] Ck=1.5;

for modes located at the point with coordinates[-1; -1]; [0; -2]; [1; -1]; [1; 1]; [-1; 1]; [0; 2] Ck=2.5;

for the remaining modes.

Amplitude-phase distribution of an element forming such a superposition presented on Fig.9.

If you ignore the amplitude component, and to consider only the phase, the distances 780-880 mm when the diameter of the element 6 mm and the diameter of the illuminating beam 4.4 mm obtained the following distribution of the light field (figure 10). Distribution obtained through equal distances from each other (20 mm). The effectiveness of this light traps 29%.

The way to move groups opaque micro-objects, which consists in shaping a light beam with closed regions of zero intensity, characterized in that the light beam of the Fort is irout of several beams, first, use three coaxial beam of the zero order Bessel with different propagation constants, forming a stable beam in the form of round spots, then these bundles are placed in the space so as to form one or more closed regions to capture and move the opaque particles.


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