Strain penicillium sp, having polyfunctional properties and realising transformations of organic remains of natural origin

FIELD: biotechnologies.

SUBSTANCE: strain Penicillium sp. PBT-2 extracted from samples of humic pod mixed with cereal straw and having polyfunctional properties and promoting increased productivity of agroecosystem, participating in processes of transformation of organic remains of natural origin, is deposited in the Departmental Collection of Beneficial Microorganisms of Agricultural Purpose of the Russian Academy of Agricultural Sciences (RCAM) (GNU VNIISHM) under the registration number RCAM 01017 and may be used in transformation of organic remains of natural origin.

EFFECT: improved productivity of agroecosystem.

3 ex

 

The invention relates to the microbiological industry, and the use of cultures of Penicillium genus Penicillium species sp. obtain a new strain of Penicillium sp., which has multifunctional properties and helps to increase the productivity of agro-ecosystems, participating in the processes of transformation of organic residues of natural origin.

Known methods of preparative chromatographic separation of complex enzyme preparations of strains producing Trichoderma sp., Penicillium sp., Chrysosporium sp., Aspergillus sp. selection of individual enzymes, inbi.ras.ru/labs/sinitsyn/index...

A method of processing sludge in a mining facility, including the stage of processing sludge microvilli of the genus Penicillium, Trichoderma, Fusarium, Phoma, Mucor, Galactomyces, Aspergillus, Botrytis, Geomyces and mixtures thereof, and culturing in a parallel tank, RU # 2303572 C2, C02F 3/34, C02F 11/02, C02F 1/72, 27.07.2007.

A known method of cleaning soil from oil pollution, providing periodic soil suspensions of microorganisms in the form of strains of micromycetes, selected from the genera Penicillium, Mucor, Aspergillus, Trichoderma, RU # 2049739 C1, C02F 3/34, 10.12.1995.

There is a method of saccharification of lignocellulosic raw materials, including raw materials preparation and processing of prepared raw enzyme preparation, which is used as the culture fluid obtained by cultivation of the strain Penicilliumverruculosum BKMF-3972D, RU # 2405838 C1, C13K 1/02, 10.12.2010.

A method of obtaining strain of the fungus. Penicillium verruculosum - producer of the enzyme encoded by the nucleotide sequence, involving the transformation of the cells of the fungus Penicillium verruculosum genetic design, RU # 2378372 C2, C12N 15/00, C12N 9/24, C12N 1/15, 10.01.2010.

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A known strain of the fungus Penicillium vermiculatum Dang.BH3P-24 for receiving the drug against phytopathogenic fungi, RU # 2322490 C1, C12N 1/14, 20.04.2008.

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Known technology for the production of technical enzymes β-glucanase (cellulases and xylanases on the basis of high-activity mutant strains producing Penicillium funiculosum and Trichoderma longibrachiautum for use as feed additives in diets of farm animals and poultry, inbi.ras.ru/labs/simtsyn/index...

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the known microorganisms of the genus Penicillium strain Penicillium sp., with the claimed properties, not found.

The present invention is based on a solution to the problem, allowing detection of the genus Penicillium strain possessing multifunctional properties and improving the productivity of agroecosystems.

The technical result of the present invention lies in the selection of the strain Penicillium sp., participating in the processes of transformation of organic residues of natural origin.

According to the invention this problem is solved due to the fact that identified a new strain of Penicillium sp.-2, which has multifunctional properties, performs the transformation of organic residues of natural origin and increases the productivity of the agroecosystem.

The applicant has not identified the sources containing information about technical solutions, identical to the present invention, which allows to make a conclusion about its compliance with the criterion of "novelty".

The applicant is not aware of any publication that would contain information about the impact of the distinctive features of invention technical result achieved. In this regard, according to the applicant, it is possible to draw a conclusion on the conformity of the proposed technical solution the criterion of "inventive step".

Received a new strain of Penicillium sp. PBT-2, which has multifunctional properties and encourages the Oia productivity of agroecosystems, participating in the processes of transformation of organic residues of natural origin.

The specific name of Penicillium sp. Thom.

The strain Penicillium sp. PBT-2 selected in July 2009 from samples of sod-podzolic soil mixed with straw grain, in the Leningrad region.

Cultural-morphological, physiological and biochemical characteristics of culture

A slow-growing strain. Microscopy (phase-contrast and light) 5-day culture in the medium of čapek showed the presence of multi-core mycelium, upright conidiophores, with branching at the top, the top row of whorls consists of failed where all disputes. Aerobe. Catalanopolonesa. On the environment of čapek appears in two days, colonies white, 3-5 days in the middle of the green, 7-day colony darken to gray-green, with a white rim, appears pigment light brown color.

The sources of carbon.

Strain uses carbohydrates for 5-7 days acidification of the environment: well - arabinose, galactose, glucose, weaker - maltose, rhamnose, sorbitol, sucrose, raffinose. Does not use dulcet.

Grows on NRM, actively thins gelatine layers from the top, the top growth. On BCH ammonia forms, nitrates restores. Starch is poorly hydrolyses. On the environment Ashby moderate growth, capable of nitrogen fixation. Grows on MPA, good the th growth MPA with glycerol. Slowly growing in liquid and agar medium of Hutchinson with cellulose (filter paper). Salt-tolerant, grows well in BCH with 2.5% and 6.5% NaCl.

Identification of the strain Penicillium sp.

The culture studied on numerous properties used in modern taxonomy.

For deposition of selected strains of microorganisms in the Departmental collection of useful microorganisms for agricultural purposes RAAS (RCAM) is additionally carried out their genetic identity.

To identify to the genus strain of micromycete was prosecution plot ITS (ITS+5S+its2 sequences). The greatest similarity identified with the genus Penicillium, 100% similarity (nearest relative Penicillium crustosum).

Identification data characterizing the strain Penicillium sp., in the figure given at the end of the description.

The culture studied on numerous properties used in modern taxonomy. The strain is identified using publications: "the soil Micromycetes" edited Bilai VI (Kiev, 1982), Raper K.B., Thom Ch. A manual of the Penicillia. Baltimore (1949), "Soil Mycology" Mirchink T. (Moscow state University, 1988), "Workshop on Microbiology, edited by Egorova NS (1976), Internet resources, etc. and referred to the genus Penicillium, type sp.

The strain Penicillium sp.-2 deposited 29.11.2011 under registration number RCAM 01017 in the Departmental collection of useful microorgan is smov agricultural purpose "State scientific institution. All-Russian research Institute of agricultural Microbiology (SSI ARRIAM RAAS)".

Example 1

The selection of the strain Penicillium sp. PBT-2

The strain Penicillium sp.-2 isolated from samples of sod-podzolic soil mixed with straw grain seed on agar nutrient medium of čapek (method of limiting dilutions, 3rd breeding).

The number of CFU (colony forming units) of the strain Penicillium sp.-2 in the sample reaches 20-30 thousand per gram soil.

Example 2

Cellulolytic activity

When growth on standard agar or liquid medium of Hutchinson strain Penicillium sp. PBT-2 has the ability to decompose cellulose (filter paper).

Among Hutchinson with filter paper make 1 ml of culture fluid.

After 3 weeks of cultivation, the culture micromycete grown on stubble agar medium of čapek, there is a change in the substrate.

Creating areas decomposition filter paper, which darken.

Determination of cellulase activity carried out on the basis of the residual amount of undigested cellulose.

The residual quantity of undigested cellulose is 20%.

Example 3

The processes of transformation of organic residues of natural origin

The strain Penicillium sp. PBT-2 is actively involved in is kilenyi organic compounds of natural origin.

The strain Penicillium sp. PBT-2 produces enzymes of class oxidoreductases - catalase, peroxidase (involved in the decomposition of organic residues, including humic substances) and polyphenoloxidase (contributing to the processes of synthesis of humic compounds) (Ashgillian. The enzymatic activity of soils Armenia. 1974. - 275 C.).

The strain Penicillium sp. PBT-2, grown on a special medium with the organic components decompose plant residues (grain straw, wood waste).

1 month is observed color change of the substrate (brown), the substrate becomes loose, easily broken. After 2-3 months see the emergence of humic substances (fulvic and humic acids in trace amounts).

The strain Penicillium sp. PBT-2 is stored in the collection LLC "Petersburg Biotechnology", 196603, St. Petersburg - Pushkin, Krasnoselskoe highway, D. 14/28, letter AB, ind. 1-N.

How to maintain and store:

The culture is grown at Chapek's medium at 25°-30°C.

Stored at a temperature of +4°C in the refrigerator.

Reseeding I once in 3 months.

Long-term storage (3 years and more) is in a frozen state at -80°C.

The proposed strain Penicillium sp. PBT-2 relates to the microbiological industry, and includes well-known methods of isolation, confirmed by research LLC "Petersburg biotech is sociology", hence, according to the applicant, compliance with a criterion of "industrial applicability".

A new strain of Penicillium sp. PBT-2 has polyfunctionality properties and increases the productivity of agroecosystems, participating in the processes of transformation of organic residues of natural origin.

Identification data of the strain Penicillllium sp.

The strain Penicillium sp., possessing multifunctional properties, performing the transformation of organic residues of natural origin and productivity of agroecosystems, deposited in the Departmental Collection of Useful Microorganisms for Agricultural Purposes RAAS (RCAM) (GNUS ARRIAM) under registration number RCAM 01017.



 

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4 tbl, 6 ex

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4 cl, 2 dwg, 1 tbl

FIELD: chemistry.

SUBSTANCE: invention relates to biotechnology. A biopreparation for cleaning aquatic media from oil and oil products is disclosed. The biopreparation is a consortium of cultures of microorganisms including biomass of cultures of bacteria Rhodococcus equi P-72-00, yeast fungus Rhodotorula glutinis 2-4 M, micellar fungus Trichoderma lignorum VKPM F-98 and microalgae Chlorella vulgaris Beijer.

EFFECT: biopreparation enables efficient cleaning of the environment from oil products, both in the oil film and in the water.

1 tbl, 2 ex

FIELD: chemistry.

SUBSTANCE: invention relates to biotechnology. A method of cleaning aquatic media from oil and oil products is disclosed. A peat-based hydrophobic oil sorbent is successively sprayed onto the surface of the contaminated water surface, followed by a biopreparation. The biopreparation is a consortium of cultures of bacteria Rhodococcus equi P-72-00, green microalgae Chlorella vulgaris Beijer, as well as yeast fungus Rhodotorula glutinis 2-4 M and/or micellar fungus Trichoderma lignorum F-98, taken in an effective amount.

EFFECT: method enables to reduce content of petroleum hydrocarbons, in 90 days, by 56-91% in water and by 25-49% in a sorbent contaminated with oil, as well as 70-74% in water and by 91-95% in a sorbent contaminated with water-soluble toxic petroleum hydrocarbons.

3 tbl, 4 ex

FIELD: chemistry.

SUBSTANCE: Pseudomonas putida 131 strain was isolated from phenol-contaminated soil by Gazprom neftekhim Salavat by culturing a soil sample in a mineralised medium, followed by collection of the most active forms, and is deposited in the Russian National Collection of Industrial Microorganisms under number B-10894.

EFFECT: invention increases efficiency of decomposing phenol compounds.

4 tbl, 3 ex

FIELD: chemistry.

SUBSTANCE: invention relates to biotechnology and specifically to an agent for biological purification of water from hydrocarbons. The biopreparation contains a porous support of biologically active microorganisms which contains glass-like metaphosphates: KPO3, Mg(PO3)2, Ca(PO3)2, Al2O3 and hydrocarbon decomposing microorganisms, and additionally contains TiO2 and inorganic nitrogen-containing compounds such as: KNO3 and NH4H2PO4 with the following ratio of ingredients, wt %: KPO3 - 20-40, Mg(PO3)2 - 5-10, Ca(PO3)2 - 30-50, Al2O3 - 5-10, TiO2 - 1-5, KNO3 - 1-3, NH4H2PO4 - the balance.

EFFECT: invention increases efficiency of the biopreparation for purifying water from hydrocarbon contaminants and also prolongs its action.

1 tbl, 2 ex

FIELD: chemistry.

SUBSTANCE: method involves adding an additive containing biomass of microorganisms into waste water with initial pH 5.5. The microorganisms used are at least one of the following types: Rhodotorula glutiuius in amount of 14-6 mg/m3, Trihoderme viridae in amount of 23-1 mg/m3, Chaetomium globosum in amount of 25-15 mg/m3.

EFFECT: invention enables to increase treatment efficiency by accelerating the process.

3 tbl

FIELD: process engineering.

SUBSTANCE: set of invention relates to water treatment. Proposed method comprises immobilisation of biomass bearing oil-oxidisers taken in effective amount into organic hydrophobic peat-based sorbent and drying it. Note here that said organic hydrophobic sorbent is prepared by low-temperature pyrolysis in vacuum of peat minced to 0.2-0.5 mm size at 210-250°C for 60-90 minutes. Said immobilisation is performed by adding sorbent into biomass suspension during its fermentation at retardation of oil-oxidising microorganisms growth. Note here that mass of added sorbent is 6-10 times larger than that of microorganisms contained in biomass suspension at the moment of sorbent application. Said oil-oxidising microorganisms represent yeast culture Candida maltosa All-Russian collection of industrial microorganisms Y-3446 or culture of bacteria Dietzia maris All-Russian collection of industrial microorganisms Ac-1824. Besides, this invention covers biosorbant for water treatment thus produced.

EFFECT: faster preparation, higher absorption capacity and efficiency.

2 cl, 4 ex

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