Biosorbent for water cleaning of hydrocarbon contamination and method of its production

FIELD: process engineering.

SUBSTANCE: set of invention relates to water treatment. Proposed method comprises immobilisation of biomass bearing oil-oxidisers taken in effective amount into organic hydrophobic peat-based sorbent and drying it. Note here that said organic hydrophobic sorbent is prepared by low-temperature pyrolysis in vacuum of peat minced to 0.2-0.5 mm size at 210-250°C for 60-90 minutes. Said immobilisation is performed by adding sorbent into biomass suspension during its fermentation at retardation of oil-oxidising microorganisms growth. Note here that mass of added sorbent is 6-10 times larger than that of microorganisms contained in biomass suspension at the moment of sorbent application. Said oil-oxidising microorganisms represent yeast culture Candida maltosa All-Russian collection of industrial microorganisms Y-3446 or culture of bacteria Dietzia maris All-Russian collection of industrial microorganisms Ac-1824. Besides, this invention covers biosorbant for water treatment thus produced.

EFFECT: faster preparation, higher absorption capacity and efficiency.

2 cl, 4 ex

 

Group of inventions relates to industrial biotechnology and ecology and can be used for purification of hydrocarbon reservoirs from pollution.

The term "biosorbent" refers sorbents, immobilized cultures of microorganisms for biological decomposition of hydrocarbons.

As carriers for immobilization of the microorganism culture can be used for virtually all organic sorbents (vegetable, synthetic and mineral origin, as well as cultures of microorganisms-destructors - any simplest hydrocarbon-oxidizing microorganisms (yeast, bacteria, fungi).

Immobilization of microorganisms on the sorbent is usually carried out by a method of fine spraying the suspension onto the surface of the sorbent with its simultaneous stirring. After drying at normal temperature biosorbent ready for use.

Biosorbent must meet the following requirements:

- to have a large sorption capacity;

to ensure the adhesion of the microorganism cells from the surface of the sorbent for a long time use (7-20 days);

- have safety for the environment and man after use;

to have hydrophobicity in the case, if used to clean water.

Currently otrabotano a significant number of biosorbents, a distinctive feature of which is the variety of media used (sorbents) and immobilized on them cultures of microorganisms.

Known bioreagent for purification of water and soil from oil pollution (RF patent No. 2081854, C02F 3/34, publ. 1997.06.20), including adapted to the hydrocarbon oil bacterial culture of Pseudomonas components and nutrient supply. As components of nutrient supply using sodium soap of synthetic fatty acids, water soluble salts of aluminum or calcium and ammonium salts of phosphoric acid. Bacterial culture together with components of the nutrient supply immobilized on gidrofobizirovannym peat in the following ratio of components, wt.%:

Bacterial culture of Pseudomonas0,29-032
Sodium soap of synthetic fatty acids3,70-of 4.38
Water-soluble aluminum salt or calcium0,97-1,59
Ammonium salt of phosphoric acid0,79-0,81
Peatrest

Known biosorbent for cleaning the water surface from oil and not the products (patent RF №2299181, C02F 3/34, publ. 2007.05.20), including meteorologie microorganisms, taken in an effective amount, and the media based on peat. As media biosorbent contains hydrophobic sorbent oil based on peat, as well as oil-oxidizing microorganisms biomass of strain micromycete: Fusarium lateritium HK-204 or Gliocladium deliquescens NC-205 or Gliocladium deliquescens HK-206 or consortium of these strains immobilized in a hydrophobic sorbent oil based on peat by fouling of the sorbent mushrooms, in the following ratio of components, wt.%:

The biomass of the strain micromycete Fusarium lateritium NC-204 or
Gliocladium deliquescens NC-205 or
Gliocladium deliquescens NC-206 or
consortium strains of micromycetes20-50
hydrophobic sorbent oil based on peatrest

Closest to the proposed biosorbent and the method of its production (prototype) is biosorbent for clean water from oil based strains of bacteria and yeast fungi (RF patent No. 2318736, C02F 3/34, publ. 2008.03.10), including nefteokislyayushchego, taken in an effective amount, and the media. As media biosorbent contains hydrophobic oil sorbent based on peat, as well as oil-oxidizing microorganisms biomass of strain of the bacterium Rhodococcus erythropolis HK-16 or Arthrobacter sp. HK-15, or yeast fungus Candida lipolytica KBP-3308 or Candida guilliermondii yeast KBP-3175 or Pichia guilliermondii yeast KBP-3205 or bacterial-yeast consortium immobilized in a hydrophobic sorbent oil based on peat by fouling of the sorbent bacteria and/or fungi, in the following ratio of components, wt.%:

The biomass of the bacterial strain:
Rhodococcus erythropolis HK-16 or
Arthrobacter sp.HK-15 or
the yeast fungus Candida lipolytica KBP-3308 or
Candida guilliermondii yeast KBP-3175 or
Pichia guilliermondii yeast KBP-3205
or bacterial-yeast consortium20-30
hydrophobic sorbent oil based on peatrest

Not what Adami known biosorbents and methods for their preparation are, first, the use of hydrocarbon-oxidizing microorganisms strains of fungi, which have a very low rate of consumption of hydrocarbons and a low reproductive rate, which leads to increase the time of recovery of hydrocarbon contamination and, secondly, the implementation of the immobilization of microorganisms on the media by fouling of the media increases the time of receipt of biosorbent (patent RF №2318736 in laboratory conditions, this time is 9 days), and also leads to the decrease of the sorption capacity of the biosorbent, because the inner pores of the carrier are sealed, which allows to use only a portion of the surface of the carrier (patent RF №2318736 sorption capacity of a biological product is 6 g of oil per 1 g of biosorbent, while currently in biosorbent have a sorption capacity of 7 g/g to 9 g/g).

The challenge which seeks the proposed group of inventions is getting biosorbent with high degree of biodegradation of hydrocarbons.

The technical result, which directed the proposed group of inventions is the reduction of time of receipt of the biosorbent, the increase in sorption capacity of the biosorbent, as well as increasing the efficiency of the process recycling of hydrocarbon contamination.

the technical result of a group of inventions is due to the fact, in the method for producing biosorbent for the treatment of water from hydrocarbon contamination, including immobilization of biomass containing taken in the effective number of meteorologie microorganisms, organic hydrophobic sorbent based on peat, followed by drying, an organic hydrophobic sorbent is produced by low-temperature pyrolysis under vacuum crushed to 0.2÷0.5 mm of peat, which is carried out at a temperature of 210÷250°C for 60÷90 minutes Immobilization is carried out by depositing the organic hydrophobic sorbent in the suspension of the biomass during the fermentation stage of slow growth of oil-oxidizing microorganisms. The mass of deposited organic hydrophobic sorbent in 6-10 times greater than the mass of oil-oxidizing microorganisms contained in the suspension of the biomass to the point of application of organic hydrophobic sorbent. As oil-oxidizing microorganisms used a strain of the yeast Candida maltosa VKPM Y-3446 or strain of bacteria Dietzia maris PMBC Ac-1824. Biosorbent for purification of water from hydrocarbon contamination, obtained by the proposed method contains as an organic hydrophobic sorbent reduced to fractions of 0.2-0.5 mm peat, and as oil-oxidizing microorganisms strains of the yeast Candida maltosa VKPM Y-3446 or strain of bacteria Dietzia maris PMBC Ac-1824.

In th is my group of inventions for organic hydrophobic sorbent use pre-shredded organic media for example peat (fractional composition of 0.2-0.5 mm), which is subjected to low-temperature pyrolysis at a temperature of 210°C to 250°C under vacuum for 60÷90 minutes In the sorbent obtained porous, with large inner surface of the contact phase. Fermentation of the biomass of yeast and/or bacteria is carried out in cumulative mode on a hydrocarbon substrate in the presence of biogenic elements in the technological parameters of growing specified in the passport of the respective cultures. Immobilization of the biomass of yeast or bacteria in organic hydrophobic sorbent is carried out in the fermentation process of the biomass of yeast or bacteria by application of organic hydrophobic sorbent suspension of biomass for 2-3 hours until the end of fermentation biomass, which reduces the time of receipt of the biosorbent. Immobilization of microorganisms is due to the fact that organic hydrophobic sorbent, hitting suspensio biomass absorbs residual hydrocarbons together with microorganisms. Under the influence of aeration and mixing in the depths of a long process of recovery of residual hydrocarbons and biomass growth of microorganisms. Growing microbial biomass deeply penetrates the pores of the sorbent, thereby ensuring the adhesion of the microorganism cells from the surface of the sorbent for a long time, as a result, the ATA which is stable immobilization of cells of microorganisms. Concentrated the resulting suspension biosorbent carried out by centrifugation, followed by drying thickened suspension of biosorbent on tape, freeze dryer or fluidized bed dryer. Received biosorbent can be used to clean water.

Below are examples of the implementation of the proposed group of inventions.

Example 1. For the preparation of organic hydrophobic sorbent use peat, which is crushed to fractions of 0.2 mm - 0.5 mm in the mill drum type. The resulting mixture of peat laid out in trays and placed in a vacuum drying Cabinet in which the set temperature of 230°C and a residual vacuum of 100 PA. After drying over 80 min organic hydrophobic sorbent is ready for use. Fermentation of hydrocarbon-oxidizing microorganisms, such as a strain of the yeast Candida maltosa VKPM Y-3446, carried out in a fermenter liquid paraffin oil C13÷C17karaminas dewaxing. The initial concentration of paraffin - 2%, the initial biomass content of yeast 2.0-2.2 g in 1 liter of suspension, mineral medium for the propagation of the strain has the following composition: H3PO4(70%) - 2.6 g/l, KCl - 1,14 g/l, MgSO4- 0.55 g/l FeSO4×7H2O - 0,045 g/l ZnSO4×7H2O - 0,031 g/l MnSO4×7H2O - 0,031 g/l CuSO4- 0,004 g/l Fermentation perform the ri pH 4.0÷4,2, the number of revolutions of the stirrer - 800 rpm, the air supply to the aeration - 100 l/h per 1 liter of the suspension of the biomass within 18 h After 18 h the biomass content of the yeast is 15 g in 1 liter of suspension. At the stage of slowing down the growth of microorganisms in the suspension of the biomass of yeast is added to 120 g of the previously obtained organic hydrophobic sorbent for every liter working volume of the fermenter (8 times the biomass of yeast grown in 18 hours). The fermentation is carried out for 3 h, until complete exhaustion of hydrocarbon supply. The resulting slurry is thickened in a centrifuge at 3500 rpm for 10 min of the Thickened suspension is spread on trays and dried in a freeze dryer for 24 h under vacuum. Drying is carried out in the temperature range from -25°C to +25°C, at a residual vacuum of 10 PA. Thus obtained biosorbent after drying, is ready for use.

Purification of water from hydrocarbon contamination as follows. In a glass vessel, pour 1 liter of water, on the surface of the water poured into 5 ml of crude oil. Then on contaminated surface water in small portions put biosorbent in an amount to provide a complete collection of oil from the water surface. Weigh biosorbent with the collected oil (in this case - is 4.85 g). Knowing the density of the oil (850 kg/m3)calculate the sorption capacity of the biosorbent, which in this the beam is about 7 g/year

Biosorbent saturated with oil, placed in a Petri dish and observe the decomposition of oil by the action of microorganisms. Biosorbent moisturize every 3 days. For 15 days the oil content decreased by 32%, and 30 days by 58%.

Example 2. Biosorbent after sorption of the oil is not removed from the water. After 20 days the amount of oil in the biosorbent decreased by 47%, which indicates a stable immobilization of oil-oxidizing microorganisms in the pores of hydrophobic organic sorbent and high degradation rate of hydrocarbons, i.e. on the process of self-cleaning biosorbent from hydrocarbons collected from the surface of the water.

Example 3. Prepare organic hydrophobic sorbent, as in example 1. Fermentation of hydrocarbon-oxidizing microorganisms, for example, a strain of bacteria Dietzia maris PMBC Ac-1824, carried out in a fermenter liquid paraffin oil With13÷17karaminas dewaxing. The initial concentration of paraffin - 2%, the initial content of the biomass of bacteria 2.0-2.2 g in 1 liter of suspension, mineral medium for the propagation of the strain has the following composition: KNO3- 4.0 g/l, KH2PO40.4 g/l, Na2HPO4×12H2O - 1.4 g/l, MgSO40.8 g/l FeSO4×7H2O - 0,045 g/l ZnSO4×7H2O - 0,031 g/l MnSO4×7H2O - 0,031 g/l CuSO4- 0,004 g/l Fermentation is carried out at pH 7.0÷7,2, the number of revolutions m is chalky - 800 rpm, the air flow of 100 l/h per 1 liter of the suspension of the biomass within 20 h After 20 h the biomass content of bacteria is 15 g in 1 liter of suspension. In the suspension of the biomass of bacteria add 120 g of the previously obtained organic hydrophobic sorbent for every liter working volume of the fermenter (8 times the biomass of microorganisms grown in 20 hours). The fermentation is carried out for 3 hours, until complete exhaustion of hydrocarbon supply. The resulting slurry is thickened in a centrifuge at 3500 rpm for 10 minutes Drying is carried out in the temperature range from -25°C to +25°C, at a residual vacuum of 10 PA.

Purification of water from hydrocarbons carried out as in example 1. Weigh biosorbent with the collected oil (in this case - 4.83 g). Considering the density of oil (850 kg/m3) calculate the adsorption capacity of the biosorbent, which in this case is around 7.3 g/g

Biosorbent saturated with oil, placed in a Petri dish and observe the decomposition of oil by the action of microorganisms. Biosorbent moisturize every 3 days. 15 days hydrocarbons decreased by 37%, and 30 days 63%.

Example 4. Biosorbent after sorption of the oil is not removed from the water. After 20 days the amount of oil in the biosorbent decreased by 51%, which indicates a stable immobilization of oil-oxidizing microorganisms in the pores of the hydrophobic organic sorbent and high speed decomposition of hydrocarbons that is, the process of self-cleaning biosorbent from hydrocarbons collected from the surface of the water.

Higher results in the purification of water from hydrocarbons and their disposal on the surface of the biosorbent were obtained by immobilization in organic hydrophobic sorbent bacterial biomass, which is explained by the pH value of water, which in the experiments cleanup is 6.5-6.8 and is closer to the optimal culture conditions for bacterial biomass than for yeast biomass.

Thus, the proposed group of inventions can reduce the time of receipt of the biosorbent, provides increased sorption capacity of the biosorbent, as well as increasing the efficiency of the process recycling of hydrocarbon contamination due to self-cleaning biosorbent from hydrocarbons.

1. A method of obtaining a biosorbent for the treatment of water from hydrocarbon contamination, including immobilization of biomass containing taken in the effective number of meteorologie microorganisms, organic hydrophobic sorbent based on peat, followed by drying, wherein the organic hydrophobic sorbent is produced by low-temperature pyrolysis under vacuum reduced to 0.2-0.5 mm of peat, which is carried out at a temperature of 210-250°C for 60-90 min, the immobilization is carried out by making organic the ski hydrophobic sorbent in the suspension of the biomass during the fermentation stage of slow growth of oil-oxidizing microorganisms, the mass of applied organic hydrophobic sorbent in 6-10 times greater than the mass of oil-oxidizing microorganisms contained in the suspension of the biomass to the point of application of organic hydrophobic sorbent, as well as oil-oxidizing microorganisms used a strain of the yeast Candida maltosa VKPM Y-3446 or strain of bacteria Dietzia maris PMBC As-1824.

2. Biosorbent for purification of water from hydrocarbon contamination, obtained by the method according to claim 1 containing as an organic hydrophobic sorbent reduced to fractions of 0.2-0.5 mm peat, and as oil-oxidizing microorganisms strain of the yeast Candida maltosa VKPM Y-3446 or strain of bacteria Dietzia maris PMBC As-1824.



 

Same patents:

FIELD: biotechnology.

SUBSTANCE: basic fermentation medium is prepared on the basis of fermentative lysate of starch in an amount of 8-15% for glucose, and additional fermentation medium containing the components of basic fermentation medium, 2-3.5 times concentrated. They are sterilised. Biosynthesis of L-lysine is carried out using strain-producer Corynebacterium glutamicum of All-Russia classification of microorganisms Ac-2577D (BIGOR 55). The fermentor is loaded with the basic nutrient medium in amount of 1/3 of its volume. Then inoculation of the basic fermentation nutrient medium is carried out with 10-20% inoculum grown in two stages. Biosynthesis process is carried out with continuous feeding starting from 8-16 hours of cultivation. At that the inhibitory concentration of reducing substances in the culture fluid of 4-8% is maintained. After filling the device to the maximum volume after 60-66 hours of cultivation and then every 6-12 hours for the rest part of biosynthesis process a part of the culture fluid in amount of 10-15% of the maximum volume is pumped into the device for final fermentation. In this device, the biosynthesis process is continued for 6-12 hours under the same conditions as in the main device but without feeding the additional nutrient medium. Then L-lysine is isolated.

EFFECT: invention enables to carry out the biosynthesis process for a long time without reducing the rate of synthesis of L-lysine, and provides obtaining of the culture fluid with high concentration of L-lysine prior to the isolation.

4 ex

FIELD: biotechnology.

SUBSTANCE: method provides sampling of the material under study. Inoculation of the material under study on nutrient medium sabouraud followed by incubation on this medium at 37°C for 24 hours. A quantitative assessment of growth of yeast-like fungi of the genus Candida, and with a value exceeding 10*1 CFU/swab the candidiasis of upper respiratory tract is diagnosed. The obtained colonies are examined under the microscope and the isolated colonies of yeast-like fungi of the genus Candida are separated from these colonies. The isolated colonies of yeast-like fungi of the genus Candida are suspended in five test tubes containing liquid medium sabouraud with the phenol red indicator, where the discs with carbohydrates are added, and in the first test tube - a disc containing maltose, in the second - sucrose, the third - lactose, the fourth-galactose, the fifth - trehalose, and are incubated at 37°C for 24 hours, followed by assessment of colour change of the indicator. The colour change of the indicator to yellow is taken as one, lack of colour change is taken as zero, the sum of the values obtained in assessment of the indicator colour in five test tubes is calculated. If the value of the sum is 0 the candidiasis of upper respiratory tract caused by Candida kruzei is diagnosed, if it is 1 - the candidiasis of the upper respiratory tract caused by Candida glabrata is diagnosed, if it is 3 - the candidiasis of the upper respiratory tract caused by Candida albicans is diagnosed, if it is 4 - the candidiasis of the upper respiratory tract caused by Candida tropicalis is diagnosed, if it is 5 - the rest.

EFFECT: invention enables to identify several types of yeast-like fungi of the genus Candida, causing candidiasis, and enables to take into account the quantitative growth of yeast-like fungi of the genus Candida, according to which the presence of candidiasis is judged.

6 ex

FIELD: biotechnology.

SUBSTANCE: strain Bacillus licheniformis CL-13 which has high histidine decarboxylase activity is deposited in the Russian National Collection of Industrial Microorganisms (RNCIM) under the registration number of RNCIM B-11185 and can be used in histidine decarboxylase of enzymatic preparation.

EFFECT: invention enables to obtain a strain of histidine decarboxylase activity.

1 ex

FIELD: biotechnologies.

SUBSTANCE: invention is an elective nutrient medium, which may be used in laboratory practice for extraction of a cholera agent. The nutrient medium contains a nutrient base, microbiological agar, saccharose, sodium chloride, sodium carbonate, bismuth nitrate pentahydrate, sodium citrate, cattle bile, EDTA iron (III)-sodium salt, bromthymol blue, mesoinositol, activated charcoal, sodium sulfite and L-cysteine, potassium tellurite and distilled water. The nutrient base in the nutrient medium is a dry nutrient broth from a pancreatic overcook of Caspian sprat or a dry nutrient broth from hydrolysate of fish and bone flour or a complex of yeast extract and peptone at the specified ratio of components.

EFFECT: invention makes it possible to increase yield of choleraic vibrio and to increase differentiating properties of the medium.

11 tbl, 11 ex

FIELD: biotechnologies.

SUBSTANCE: method includes clarification of a cellular culture liquid by low-speed centrifugation at the speed of lower than 10000 rpm or with the help of a module of volume filtration, the pore size of which makes from 1.0 mcm to 4.5 mcm, to produce a vesicular stomatitis virus in a supernatant. The supernatant is filtered via a filter with holes of the diameter of 0.2-0.45 mcm, and the vesicular stomatitis virus is produced in the filtered solution. The filtered solution, which contains the vesicular stomatitis virus, is loaded to an anion-exchange membrane adsorber balanced with the first buffer salt solution. The first buffer salt solution has ion force from 100 mM to 400 mM. The vesicular stomatitis virus is eluted from the anion-exchange membrane adsorber with the second buffer salt solution. The vesicular stomatitis virus is eluted from the membrane adsorber, when the second buffer salt solution is added, in one stage. Concentration of salt in the single-stage elution makes from 500 mM to 750 mM. Eluted fractions containing the vesicular stomatitis virus are collected. The vesicular stomatitis virus is cleaned with running filtration along the flow direction (press filter) using the membrane, the transmission limit of which is from 300 kDa to 1000 kDa, and the concentrate of the vesicular stomatitis virus is produced. The concentrate containing the vesicular stomatitis virus is filtered via a filter with holes of the diameter of 0.2-0.22 mcm, and the vesicular stomatitis virus is produced in the filtered solution. The method of cleaning does not include use of human embryos.

EFFECT: invention makes it possible to produce a vesicular stomatitis virus of high extent of cleaning with high yield.

13 cl, 8 dwg, 33 tbl, 12 ex

FIELD: biotechnologies.

SUBSTANCE: invention may be used to produce a new biopreparation for cleaning of water, soil, industrial drains from pesticides resistant to decomposition and selected from chlorophenoxyacetic acids, such as 2,4-dichlorophenoxyacetic acid (2,4-D), trichlorophenoxyacetic acid (2,4,5-T), chlorophenoxyacetic acid (CPAA), phenoxyacetic acid (PAA), - 2,4-dichlorophenoxy-α-propionic acid, 2-methyl-4-chlorophenoxy-α-propionic acid, 2,4,5-trichlorophenoxy-α-propionic acid, 2,4-dichlorophenoxy-α-butyric acid, methyl-[1-(butylamino) carbonyl]-1H-benzimidazol-2-ylcarbamate, 2,4-dichlorophenol, imidoclapride, hexachlorohexane, and also phenol. The biopreparation is an association of strains of bacteria Pseudomonas putida VKPM V-10997, Bacillus cubtilis VKPM V-10999 and Rhodococcus erythropolis VKPM - As-1882 at the weight ratio of (1-2):(1-2):1. At the same the biopreparation, as a rule, contains additionally a sorbent, organic, mineral and stimulating additives and has activity that stimulates growth of plants and fungicide properties. The produced biopreparation is introduced as an aqueous solution by means of sprinkling into polluted soil or industrial drain in efficient amount.

EFFECT: invention makes it possible to improve cleaning of water, soil, industrial drains from pesticides resistant to decomposition.

21 tbl, 29 ex

FIELD: biotechnologies.

SUBSTANCE: invention may be used to produce a new biopreparation for cleaning of water, soil, industrial drains from pesticides resistant to decomposition and selected from chlorophenoxyacetic acids, such as 2,4-dichlorophenoxyacetic acid (2,4-D), trichlorophenoxyacetic acid (2,4,5-T), chlorophenoxyacetic acid (CPAA), phenoxyacetic acid (PAA), - 2,4-dichlorophenoxy-α-propionic acid, 2-methyl-4-chlorophenoxy-α-propionic acid, 2,4,5-trichlorophenoxy-α-propionic acid, 2,4-dichlorophenoxy-α-butyric acid, methyl-[1-(butylamino) carbonyl]-1H-benzimidazol-2-ylcarbamate, 2,4-dichlorophenol, imidoclapride, hexachlorohexane, and also phenol. The biopreparation is an association of strains of bacteria Pseudomonas putida VKPM V-10997, Bacillus cubtilis VKPM V-10999 and Rhodococcus erythropolis VKPM - As-1882 at the weight ratio of (1-2):(1-2):1. At the same the biopreparation, as a rule, contains additionally a sorbent, organic, mineral and stimulating additives and has activity that stimulates growth of plants and fungicide properties. The produced biopreparation is introduced as an aqueous solution by means of sprinkling into polluted soil or industrial drain in efficient amount.

EFFECT: invention makes it possible to improve cleaning of water, soil, industrial drains from pesticides resistant to decomposition.

21 tbl, 29 ex

FIELD: biotechnologies.

SUBSTANCE: invention may be used to produce a new biopreparation for cleaning of water, soil, industrial drains from pesticides resistant to decomposition and selected from chlorophenoxyacetic acids, such as 2,4-dichlorophenoxyacetic acid (2,4-D), trichlorophenoxyacetic acid (2,4,5-T), chlorophenoxyacetic acid (CPAA), phenoxyacetic acid (PAA), - 2,4-dichlorophenoxy-α-propionic acid, 2-methyl-4-chlorophenoxy-α-propionic acid, 2,4,5-trichlorophenoxy-α-propionic acid, 2,4-dichlorophenoxy-α-butyric acid, methyl-[1-(butylamino) carbonyl]-1H-benzimidazol-2-ylcarbamate, 2,4-dichlorophenol, imidoclapride, hexachlorohexane, and also phenol. The biopreparation is an association of strains of bacteria Pseudomonas putida VKPM V-10997, Bacillus cubtilis VKPM V-10999 and Rhodococcus erythropolis VKPM - As-1882 at the weight ratio of (1-2):(1-2):1. At the same the biopreparation, as a rule, contains additionally a sorbent, organic, mineral and stimulating additives and has activity that stimulates growth of plants and fungicide properties. The produced biopreparation is introduced as an aqueous solution by means of sprinkling into polluted soil or industrial drain in efficient amount.

EFFECT: invention makes it possible to improve cleaning of water, soil, industrial drains from pesticides resistant to decomposition.

21 tbl, 29 ex

FIELD: biotechnologies.

SUBSTANCE: strain of bacterial Pseudomonas panipatensis VKPM V-10593 is proposed, which is capable of quick recycling of oil and oil products, in particular, diesel fuel. Strain may be used to clean soil and water reservoirs contaminated with oil and oil products, in a wide range of temperatures from +8 to +30°C.

EFFECT: improved properties of a strain.

2 tbl, 5 ex

FIELD: biotechnologies.

SUBSTANCE: method provides for degreasing of buckwheat straw with 100% acetone. The degreased straw is extracted in 5 l of 70% ethyl alcohol in boiling water bath for 1 hour. The extracted raw materials are separated by filtration to produce alcohol extract. The produced alcohol extract of the stimulant is concentrated under vacuum, cleaned from admixtures with ethyl acetate and dried to prepare target product.

EFFECT: invention makes it possible to increase yield of yeast biomass.

2 tbl, 2 ex

FIELD: biotechnology.

SUBSTANCE: basic fermentation medium is prepared on the basis of fermentative lysate of starch in an amount of 8-15% for glucose, and additional fermentation medium containing the components of basic fermentation medium, 2-3.5 times concentrated. They are sterilised. Biosynthesis of L-lysine is carried out using strain-producer Corynebacterium glutamicum of All-Russia classification of microorganisms Ac-2577D (BIGOR 55). The fermentor is loaded with the basic nutrient medium in amount of 1/3 of its volume. Then inoculation of the basic fermentation nutrient medium is carried out with 10-20% inoculum grown in two stages. Biosynthesis process is carried out with continuous feeding starting from 8-16 hours of cultivation. At that the inhibitory concentration of reducing substances in the culture fluid of 4-8% is maintained. After filling the device to the maximum volume after 60-66 hours of cultivation and then every 6-12 hours for the rest part of biosynthesis process a part of the culture fluid in amount of 10-15% of the maximum volume is pumped into the device for final fermentation. In this device, the biosynthesis process is continued for 6-12 hours under the same conditions as in the main device but without feeding the additional nutrient medium. Then L-lysine is isolated.

EFFECT: invention enables to carry out the biosynthesis process for a long time without reducing the rate of synthesis of L-lysine, and provides obtaining of the culture fluid with high concentration of L-lysine prior to the isolation.

4 ex

FIELD: biotechnology.

SUBSTANCE: method provides sampling of the material under study. Inoculation of the material under study on nutrient medium sabouraud followed by incubation on this medium at 37°C for 24 hours. A quantitative assessment of growth of yeast-like fungi of the genus Candida, and with a value exceeding 10*1 CFU/swab the candidiasis of upper respiratory tract is diagnosed. The obtained colonies are examined under the microscope and the isolated colonies of yeast-like fungi of the genus Candida are separated from these colonies. The isolated colonies of yeast-like fungi of the genus Candida are suspended in five test tubes containing liquid medium sabouraud with the phenol red indicator, where the discs with carbohydrates are added, and in the first test tube - a disc containing maltose, in the second - sucrose, the third - lactose, the fourth-galactose, the fifth - trehalose, and are incubated at 37°C for 24 hours, followed by assessment of colour change of the indicator. The colour change of the indicator to yellow is taken as one, lack of colour change is taken as zero, the sum of the values obtained in assessment of the indicator colour in five test tubes is calculated. If the value of the sum is 0 the candidiasis of upper respiratory tract caused by Candida kruzei is diagnosed, if it is 1 - the candidiasis of the upper respiratory tract caused by Candida glabrata is diagnosed, if it is 3 - the candidiasis of the upper respiratory tract caused by Candida albicans is diagnosed, if it is 4 - the candidiasis of the upper respiratory tract caused by Candida tropicalis is diagnosed, if it is 5 - the rest.

EFFECT: invention enables to identify several types of yeast-like fungi of the genus Candida, causing candidiasis, and enables to take into account the quantitative growth of yeast-like fungi of the genus Candida, according to which the presence of candidiasis is judged.

6 ex

FIELD: biotechnology.

SUBSTANCE: strain Bacillus licheniformis CL-13 which has high histidine decarboxylase activity is deposited in the Russian National Collection of Industrial Microorganisms (RNCIM) under the registration number of RNCIM B-11185 and can be used in histidine decarboxylase of enzymatic preparation.

EFFECT: invention enables to obtain a strain of histidine decarboxylase activity.

1 ex

FIELD: biotechnologies.

SUBSTANCE: invention is an elective nutrient medium, which may be used in laboratory practice for extraction of a cholera agent. The nutrient medium contains a nutrient base, microbiological agar, saccharose, sodium chloride, sodium carbonate, bismuth nitrate pentahydrate, sodium citrate, cattle bile, EDTA iron (III)-sodium salt, bromthymol blue, mesoinositol, activated charcoal, sodium sulfite and L-cysteine, potassium tellurite and distilled water. The nutrient base in the nutrient medium is a dry nutrient broth from a pancreatic overcook of Caspian sprat or a dry nutrient broth from hydrolysate of fish and bone flour or a complex of yeast extract and peptone at the specified ratio of components.

EFFECT: invention makes it possible to increase yield of choleraic vibrio and to increase differentiating properties of the medium.

11 tbl, 11 ex

FIELD: biotechnologies.

SUBSTANCE: method includes clarification of a cellular culture liquid by low-speed centrifugation at the speed of lower than 10000 rpm or with the help of a module of volume filtration, the pore size of which makes from 1.0 mcm to 4.5 mcm, to produce a vesicular stomatitis virus in a supernatant. The supernatant is filtered via a filter with holes of the diameter of 0.2-0.45 mcm, and the vesicular stomatitis virus is produced in the filtered solution. The filtered solution, which contains the vesicular stomatitis virus, is loaded to an anion-exchange membrane adsorber balanced with the first buffer salt solution. The first buffer salt solution has ion force from 100 mM to 400 mM. The vesicular stomatitis virus is eluted from the anion-exchange membrane adsorber with the second buffer salt solution. The vesicular stomatitis virus is eluted from the membrane adsorber, when the second buffer salt solution is added, in one stage. Concentration of salt in the single-stage elution makes from 500 mM to 750 mM. Eluted fractions containing the vesicular stomatitis virus are collected. The vesicular stomatitis virus is cleaned with running filtration along the flow direction (press filter) using the membrane, the transmission limit of which is from 300 kDa to 1000 kDa, and the concentrate of the vesicular stomatitis virus is produced. The concentrate containing the vesicular stomatitis virus is filtered via a filter with holes of the diameter of 0.2-0.22 mcm, and the vesicular stomatitis virus is produced in the filtered solution. The method of cleaning does not include use of human embryos.

EFFECT: invention makes it possible to produce a vesicular stomatitis virus of high extent of cleaning with high yield.

13 cl, 8 dwg, 33 tbl, 12 ex

FIELD: biotechnologies.

SUBSTANCE: invention may be used to produce a new biopreparation for cleaning of water, soil, industrial drains from pesticides resistant to decomposition and selected from chlorophenoxyacetic acids, such as 2,4-dichlorophenoxyacetic acid (2,4-D), trichlorophenoxyacetic acid (2,4,5-T), chlorophenoxyacetic acid (CPAA), phenoxyacetic acid (PAA), - 2,4-dichlorophenoxy-α-propionic acid, 2-methyl-4-chlorophenoxy-α-propionic acid, 2,4,5-trichlorophenoxy-α-propionic acid, 2,4-dichlorophenoxy-α-butyric acid, methyl-[1-(butylamino) carbonyl]-1H-benzimidazol-2-ylcarbamate, 2,4-dichlorophenol, imidoclapride, hexachlorohexane, and also phenol. The biopreparation is an association of strains of bacteria Pseudomonas putida VKPM V-10997, Bacillus cubtilis VKPM V-10999 and Rhodococcus erythropolis VKPM - As-1882 at the weight ratio of (1-2):(1-2):1. At the same the biopreparation, as a rule, contains additionally a sorbent, organic, mineral and stimulating additives and has activity that stimulates growth of plants and fungicide properties. The produced biopreparation is introduced as an aqueous solution by means of sprinkling into polluted soil or industrial drain in efficient amount.

EFFECT: invention makes it possible to improve cleaning of water, soil, industrial drains from pesticides resistant to decomposition.

21 tbl, 29 ex

FIELD: biotechnologies.

SUBSTANCE: invention may be used to produce a new biopreparation for cleaning of water, soil, industrial drains from pesticides resistant to decomposition and selected from chlorophenoxyacetic acids, such as 2,4-dichlorophenoxyacetic acid (2,4-D), trichlorophenoxyacetic acid (2,4,5-T), chlorophenoxyacetic acid (CPAA), phenoxyacetic acid (PAA), - 2,4-dichlorophenoxy-α-propionic acid, 2-methyl-4-chlorophenoxy-α-propionic acid, 2,4,5-trichlorophenoxy-α-propionic acid, 2,4-dichlorophenoxy-α-butyric acid, methyl-[1-(butylamino) carbonyl]-1H-benzimidazol-2-ylcarbamate, 2,4-dichlorophenol, imidoclapride, hexachlorohexane, and also phenol. The biopreparation is an association of strains of bacteria Pseudomonas putida VKPM V-10997, Bacillus cubtilis VKPM V-10999 and Rhodococcus erythropolis VKPM - As-1882 at the weight ratio of (1-2):(1-2):1. At the same the biopreparation, as a rule, contains additionally a sorbent, organic, mineral and stimulating additives and has activity that stimulates growth of plants and fungicide properties. The produced biopreparation is introduced as an aqueous solution by means of sprinkling into polluted soil or industrial drain in efficient amount.

EFFECT: invention makes it possible to improve cleaning of water, soil, industrial drains from pesticides resistant to decomposition.

21 tbl, 29 ex

FIELD: biotechnologies.

SUBSTANCE: invention may be used to produce a new biopreparation for cleaning of water, soil, industrial drains from pesticides resistant to decomposition and selected from chlorophenoxyacetic acids, such as 2,4-dichlorophenoxyacetic acid (2,4-D), trichlorophenoxyacetic acid (2,4,5-T), chlorophenoxyacetic acid (CPAA), phenoxyacetic acid (PAA), - 2,4-dichlorophenoxy-α-propionic acid, 2-methyl-4-chlorophenoxy-α-propionic acid, 2,4,5-trichlorophenoxy-α-propionic acid, 2,4-dichlorophenoxy-α-butyric acid, methyl-[1-(butylamino) carbonyl]-1H-benzimidazol-2-ylcarbamate, 2,4-dichlorophenol, imidoclapride, hexachlorohexane, and also phenol. The biopreparation is an association of strains of bacteria Pseudomonas putida VKPM V-10997, Bacillus cubtilis VKPM V-10999 and Rhodococcus erythropolis VKPM - As-1882 at the weight ratio of (1-2):(1-2):1. At the same the biopreparation, as a rule, contains additionally a sorbent, organic, mineral and stimulating additives and has activity that stimulates growth of plants and fungicide properties. The produced biopreparation is introduced as an aqueous solution by means of sprinkling into polluted soil or industrial drain in efficient amount.

EFFECT: invention makes it possible to improve cleaning of water, soil, industrial drains from pesticides resistant to decomposition.

21 tbl, 29 ex

FIELD: biotechnologies.

SUBSTANCE: strain of bacterial Pseudomonas panipatensis VKPM V-10593 is proposed, which is capable of quick recycling of oil and oil products, in particular, diesel fuel. Strain may be used to clean soil and water reservoirs contaminated with oil and oil products, in a wide range of temperatures from +8 to +30°C.

EFFECT: improved properties of a strain.

2 tbl, 5 ex

FIELD: biotechnologies.

SUBSTANCE: method provides for degreasing of buckwheat straw with 100% acetone. The degreased straw is extracted in 5 l of 70% ethyl alcohol in boiling water bath for 1 hour. The extracted raw materials are separated by filtration to produce alcohol extract. The produced alcohol extract of the stimulant is concentrated under vacuum, cleaned from admixtures with ethyl acetate and dried to prepare target product.

EFFECT: invention makes it possible to increase yield of yeast biomass.

2 tbl, 2 ex

FIELD: biotechnologies.

SUBSTANCE: invention may be used to produce a new biopreparation for cleaning of water, soil, industrial drains from pesticides resistant to decomposition and selected from chlorophenoxyacetic acids, such as 2,4-dichlorophenoxyacetic acid (2,4-D), trichlorophenoxyacetic acid (2,4,5-T), chlorophenoxyacetic acid (CPAA), phenoxyacetic acid (PAA), - 2,4-dichlorophenoxy-α-propionic acid, 2-methyl-4-chlorophenoxy-α-propionic acid, 2,4,5-trichlorophenoxy-α-propionic acid, 2,4-dichlorophenoxy-α-butyric acid, methyl-[1-(butylamino) carbonyl]-1H-benzimidazol-2-ylcarbamate, 2,4-dichlorophenol, imidoclapride, hexachlorohexane, and also phenol. The biopreparation is an association of strains of bacteria Pseudomonas putida VKPM V-10997, Bacillus cubtilis VKPM V-10999 and Rhodococcus erythropolis VKPM - As-1882 at the weight ratio of (1-2):(1-2):1. At the same the biopreparation, as a rule, contains additionally a sorbent, organic, mineral and stimulating additives and has activity that stimulates growth of plants and fungicide properties. The produced biopreparation is introduced as an aqueous solution by means of sprinkling into polluted soil or industrial drain in efficient amount.

EFFECT: invention makes it possible to improve cleaning of water, soil, industrial drains from pesticides resistant to decomposition.

21 tbl, 29 ex

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