"vitaflor-p" dry probiotically active preparation

FIELD: food industry.

SUBSTANCE: dry probiotically active biopreparation contains Vitaflor" starter in the form of a porous tablet (with pores sized no less than 50% and activity no lower than 5x10 CFU/ tablet) and powder of sublimation-dried drinking milk with fat content of 1.5-2.5%. Tablet production involves dispensing in aluminium moulds, cooling to a temperature equal to minus 35-45°C in a 6-7 mm thick layer with subsequent maintenance at a temperature of minus 30-35°C. Sublimation drying is performed at the condenser-freezer temperature equal to no higher than minus 50°C under the working chamber pressure equal to 13-15 Pa during 48-50 hours. The crystallised moisture sublimation period lasts 20 hours at the material temperature equal to no higher than 30°C; additional drying is performed during 8 hours at a temperature of 25°C. One produces a tablet with 19.0±1.0 mm diameter and 6.5±0.5 mm height.

EFFECT: invention ensures a high probiotic potential of the biopreparation under extreme conditions, in particular, on board a space ship as well as under conditions of long-term self-sustained underwater navigation and the possibility of production of a high quality cultured beverage with probiotic properties and immunomodulatory effect.

3 cl, 4 dwg, 2 tbl, 8 ex

 

The invention relates to the field of biotechnology, and in particular to compositions for preparation of a fermented milk product with a high probiotic potential, in extreme conditions, in particular on Board a space ship or station, in conditions of long-term Autonomous diving, etc. It can also be used in food industry, medicine, veterinary science and related industries.

Known dry biological products based on lactic acid microorganisms (EP 0097484, 1984: EP 0043962, 1980; US 4289888, 1979). Such preparations contain cells of bacteria, the remnants of the cultural liquid (QL), fat, protein, lactose, gum and other additives. The technology of their receipt includes, as a rule, the preparation of a solution or suspension of the biomass of microorganisms, the introduction of its special additives, freeze-drying and getting the finished dry product (yeast)used in the following as inoculum in obtaining milk product using fermentors or other similar equipment.

The disadvantage of the above technologies is relatively low biological activity of seed material, technological complexity, and the need to use special equipment for the beverage, which is difficult in extreme conditions is x due to lack of available space and existing restrictions on weight equipment.

One of the most promising probiotic preparations for use in extreme conditions due to ease of access and a wide range of positive effects on the body is the drug "PICAFLOR"® (RU 2160992, 2000). Obtained on the basis of the drink proved itself as an effective antibacterial agent for the prevention and treatment of intestinal infections, certain cancers, as well as promising immunostimulant and adaptogen (EN 2269353, 2006; EN 2170023, 2001), along with full safety - drug permitted for babies older than 3 months - makes it promising for use in extreme conditions, when a person is deprived of the opportunity to benefit from inpatient treatment. The method of obtaining drink is that freeze dry leaven of this drug, which contains 2-100 billion viable cells of 2 strains of Lactobacillus acidophilus N-1 and N-2, 0.5-2.5 wt.% gelatin, 5-20 wt.% sucrose, 3-5 wt.% skim milk, 2-8 wt.% water and the remains of the culture fluid is placed in the milk or milk mixture and maintained at an elevated temperature for 16-18 hours

The disadvantages of this drug and the production technology can be attributed to the fact that to obtain the final fermented product having the desired probiotic properties and, you must have significant amounts of fresh milk, which is difficult in conditions of prolonged isolation from sources of supply. However, the use of standard milk powder obtained by spray drying, it is not possible to get a quality product with a high titer of living organisms

A known technology for leaven "Picaflor" in the form of tablets using trituration technology, the essence of which is to spill seed with a nutrient medium and forming and auxiliary additives (sodium alginate, polyglycine, beet juice and mahasweta) matrix-sterile aluminum forms with a diameter of 20 mm and a height of 7 mm, and then was cooled to -10°C and placed in the freeze-drying chamber at 2.0-2.5 hours at 25-27°C, and then the temperature was lowered to-47-50°C. while reducing the pressure to 10-12 PA and stood for hours 33-43 with the gradual rise of temperature up to 25-27°C and pressure up to 5-6 PA. When a camera these conditions form exhibited 3 hours, then dumped the vacuum and was carried out by discharging the tablets form (RU 2169574, 2001).

The benefits of probiotic preparation obtained in this way is stable when stored at positive temperatures. The disadvantages of drugs is but a poor water-solubility, as well as the introduction to his composition of the substances not indicated for use in conditions of weightlessness, as well as the necessity in obtaining milk-based product to use significant amounts of liquid nutrient medium complex composition, which is difficult in terms of restrictions on weight and dimensions product used.

The closest to the technical nature of the claimed invention is previously developed by the authors of the drug for use in extreme conditions - "Picaflor-K (RU 2425576, 2011), representing a mixture of freeze-dried powders of the leaven "Vilaflor and nutrient-mix dry milk with 0.1 to 1.0% of surface-active substances (surfactants), placed in a hermetically sealed container. The original product (a mixture of powders of medium and inoculum) for later retrieval on the basis of a fermented milk drink is prepared by mixing in a ratio of freeze-dried and ground leaven "Picaflor", surfactant and freeze-dried, and powdered milk. To obtain the final dairy product container is opened, the preparation is diluted with water to a given concentration (typically in a ratio of about 1:10) and place on 18-20 hours in a thermostat at 37±2°C.

The disadvantages is the possibility of the loss of activity of the microbial product during transport of the drug into orbit at temperatures reaching the values of 25-27°C and above (e.g., during delivery of the drug to the ISS, where you intend to use it as a starter culture to obtain a fermented milk product "Picaflor") and its subsequent storage. Such deactivation of the drug stems from the fact that during the grinding of dried biological product consisting of cells of microorganisms observed a significant increase in its specific surface area and increased availability of bacterial cells to outside influence from both oxygen and water, especially manifested during contact with a nutrient medium containing a certain number of bound water. The result is a significant wetting of the dry material and, as a consequence, uncontrolled reduction of biological activity of accumulating product during its storage, which may negatively affect the future possibility of receiving on the basis of dairy product and its quality, as the transportation of dry mixture and stored until use often performed (as was shown in a live experiment, when the dried product at a temperature of 37°C for 2 weeks (test "accelerated storage), biological activity "Picaflor", part of the dry mix, reduced 2,1±0,2×109The OE/g to 0.07±0,01×10 9CFU/g, i.e. more than 100 times. Especially noticeable decline in the activity of bacteria when replacing in this composition "Picaflor" on probiotic preparations containing other microorganisms).

The disadvantages of this drug can be attributed also to the fact that during the grinding of dried biological product consisting of cells of microorganisms by increasing its specific surface area can be significant wetting of the dry material and, as a consequence, uncontrolled reduction of biological activity of accumulating product during its subsequent storage in the field of positive temperatures. As was shown in a live experiment, when the dried product at a temperature of 37°C for 2 weeks (test "rapid storage"), the biological activity of the crushed yeast probiotic "Picaflor", part of the dry mix, reduced from 2.1±0,2×109CFU/g to 0.07±0,01×109CFU/g, i.e. about 30 times.

The problem to be solved by the authors, was the creation of technology for dry preparation on the basis of the leaven "Picaflor", more stable to the influence of external factors and allowing for the dilution of dry inoculant water and sticking it to 18 hours. in a thermostat at 37°C are guaranteed to receive high-quality dairy prod is, with probiotic properties and immunomodulatory effects.

To solve this problem it was proposed to develop a technology transfer contained in the preparation of the starter culture in a more protected from the external environment form, in particular in the form of tablets. Together with the tablet form of the drug was easily destroyed by mechanical action and quickly dissolve in the aquatic environment.

The technical result was achieved by the creation of the song "Picaflor-P", which, along with freeze-dried and powdered milk contained dry leaven "Picaflor" in the form of tablets having internal pores constituting at least 50% of volume pills and the obtained modified trituration method. To do this, in the formation of tablets nutrient medium containing microorganisms poured into aluminum forms volume 2.0±0.05 ml layer of 6-7 mm, pre-cooled to a temperature of minus 35 to minus 45°C, kept at a temperature of minus 30-35°C, and then subjected to freeze-drying at a temperature not higher than minus 50°C and a pressure of 13 to 15 PA for 48-50 hours.

It was shown that the storage efficiency of the drug "Picaflor-P" rises further with the introduction of the nutrient medium before dehydration 5-10% of ascorbic acid per end with the Hoi product.

The distinction of the proposed treatment technologies leaven from its technology, described in the next similar and from the previously described trituration technology, is that in the present case, the composition is subjected to rapid freezing throughout the volume of the liquid due to exposure to low temperature on its entire surface, including the side. Such exposure leads to rapid crystallization of the frozen liquid medium and the formation of a large number of vertical and horizontal channels (see figure 3), the total volume in the resulting tablet is more than 50% of the volume.

The presence of such channels on the one hand allows to use compared to trituration more sparing methods for microorganisms mode dehydration, and on the other hand provides quick dissolving tablets in water and faster contact of microorganisms with protective casein medium (casein of milk, forming a "protective capsule around the colonies of microorganisms), creating optimal conditions for their further cultivation.

Thus, significant differences from the analog - triturations technology is the lack of poliglyukina and other formative elements, and the use of rapid cooling to minus 30 to minus 40°C compared to the proposed analogue of the primary cooling is (up to minus 10°C).

Compared with the technology of fermentation in the preparation Vilaflor To" major differences are the formation of the target product bulk freezing of small fragments of a liquid medium with the formation of pills and the possibility of applying a more strict regime of dehydration. So, time sublimation crystallized moisture is 20 hours at a temperature of the material above 30°C, and final drying is carried out for 8 h at 25°C.

As mentioned above, during the research it was found that such treatment occurs in the presence of a relatively small external surface formation trunk vertical and radial pores, which make up more than 50% of the volume of the pill, which makes it easy chopping with external mechanical impact and rapid dissolution (time to complete dissolution of the tablets does not exceed 30 s).

The resulting composition to obtain a lactic acid beverage in extreme conditions was a package containing a porous tablet leaven "Picaflor", weight is 0.260±0,010 g (has a diameter 19,0±1.0 mm and a height of 6.5±0.5 mm) and powder of dried milk in the amount of 12.5±0,30, As shown by the test method of accelerated storage, the biological activity of the composition, based tablets containing leaven, the processing is subjected to the above image, storage of Vilaflor-P" for 2 weeks at 37°C did not lead to appreciable inactivation of stored biological product. So the biological activity of the tablets decreased from 3.2±0,6×109CFU/g to 1.1±0,25 CFU/g dry product, which gives grounds to assume that after transportation to orbit the drug Vilaflor-P" keeps quasifeudal ability after rehydration drinking water. Another advantage of the inventive technology is the possibility of applying this method to obtain lactic acid drinks with other species of lactobacilli using homofermentative type of fermentation, for example, Lactobacillus helveticus.

Figure 1 shows a General view of the inventive packaging "Picaflor-P; figure 2 shows the appearance of the tablets obtained according to the technology of freeze-molding; figure 3 is a micrograph of the cross-section of the tablet.

The invention is illustrated by the following examples.

Example 1. Getting the leaven. In ampoules with liofilizovannye standards of industrial strains of Lactobacillus acidophilus H-1 and H-2 make 1 ml of distilled water, and then mix thoroughly, make separately in tubes with skim milk, and incubated at 37°C for 16-20 hours. The seed material is injected into the flask containing 100 ml hydrolysate-dairy environment containing pancre the political hydrolysate of milk 0.2 g of peptone, 0.06 g of sodium chloride, 0.002 g of cysteine and 0.1 g of lactose and incubated at 37°C for 16-20 hours. Finished planting material was obtained by mixing the separately grown cultures so that the ratio of cells of the individual strains was close to 1:1 according to optical density at wavelength 600 nm.

Growing native culture is produced in the medium containing 1 l of pancreatic hydrolysate of milk, 2 g peptone, 0.6 g of sodium chloride, 0.02 g of cysteine, 4 g of calcium carbonate and 1 g of lactose at 37°C for 16-20 hours. To the culture fluid, add if necessary ascorbic acid in the calculation of 5-10% of the mass. on the final product.

The resulting product is poured into cooled to 30±5°C aluminum forms 2.0±0.05 ml in each, what is the thickness of the dried layer 6 mm, and then placed in a chamber of a pilot freeze-install alpha 1-20 ("chryst", Germany) at minus 30-35°C for 1-2 hours, and then subjected to freeze-drying at a temperature of condenser-memoratives not higher than minus 50°C and the pressure in the freeze-drying chamber is 13-15 PA. The time to reach the working vacuum is 20-30 minutes, the total duration of the drying process - 48-50 hours.

The duration of stage sublimation crystallized moisture is 20 hours at a temperature of the material above 30°C, the final drying Khujand is realized within 8 h at 25°C.

Obtained after drying the product has the appearance of uniform color and height of the tablet (diameter : 19,0±1.0 mm, the height is 6.5±0.5 mm). The appearance of the tablets obtained according to the technology of freeze-molding shown in figure 1. The biological activity of the tablets is 5.6×109CFU/tablet.

The resulting technology of freeze-forming porous tablets are stored in a tightly closed container at a temperature of 5 to 6°C, guaranteed shelf life of the tablets is 2 years.

Example 2. In the conditions of example 1 are as leaven in the introduction to the culture medium of ascorbic acid in the amount of 8% of the mass. calculated on the final product. The biological activity of the tablets is 2.8±109CFU/tablet.

Example 3. In the conditions of example 1 are as leaven in the introduction to the culture medium of ascorbic acid in an amount of 5% of the mass. calculated on the final product. The biological activity of the tablets is 2.0×109CFU/tablet.

Example 4. In the conditions of example 1 get the starter without the introduction into the medium of ascorbic acid. The biological activity of the tablets is 0.6×109CFU/tablet.

Example 5. The product "Picaflor-P". Drinking milk is poured into aluminum trays, 300 ml each (thickness of dried layer is 3-4 mm) and subjected to freeze on the shelf of Sublim the operating installation TG-5 (Germany) at a temperature of minus 25 minus 30°C for 1.5 to 2.0 hours and then freeze-dried at a temperature not higher than minus 20°C and a pressure of 15-18 PA for at least 48 hours. Upon completion of the drying process liofilizirovannoe milk ground screw on the chopper.

When preparing the product Vilaflor-P" you used the following ratio of components: freeze-dried powdered milk - 12.5 g; obtained according to the technology of sublimation forming a porous tablet probiotic "Vilaflor containing 2 strains of lactobacilli, obtained in the conditions of example 1-0,260, the Obtained sample dry nutrient medium (freeze-dried and ground drinking milk brand "Full pot" fat 1,5%) packaged in sealed containers. There was placed a porous pills "Picaflor" (1 tablet each), after which the vessel was germetizirovany.

To obtain a fermented milk drink "Vilaflor in the container with the product "Picaflor-P" was added 60 ml of drinking water. Capacity with rehydration material was covered and placed in a thermostat heated to 37±2°C for 18 hours For achieving the above exposition of the obtained milk product was removed from thermostat and checked for the presence of probiotic properties: biological activity, antibiotic resistance, antagonism against pathogenic and y is o-pathogenic microorganisms, kislotoobrazovanie. The biological activity of the obtained fermented milk product amounted to 4×10 CFU/ml, the pH of the obtained product was equal to 3.4. The antibiotic resistance and antagonism is consistent with the rate declared for probiotic "Picaflor".

Example 6. Composition "Picaflor-P, obtained in the conditions of example 4 were subjected to storage at room temperature for 1 month, after which he determined the biological activity of the dry composition. Then dry composition was recitatives by adding 60 ml of drinking water and cultured in an incubator at 37°C for 18 hours In conclusion, the experience was defined biological activity of the obtained fermented milk product. For comparison, in the same conditions of temperature stored samples (control), where the inoculum was used powdered drug "Picaflor-K and compositions containing the yeast obtained according to examples 2 and 3. The percentage ratio between the components: feed / seed product "Picaflor-P was similar for control and experimental samples. The results are given in table 1.

Table 1.
The influence of the shape of the leaven of the biological activity regerative the aqueous dairy product (original composition: 0.26 g of yeast probiotic; 12.5 g of the nutrient medium is crushed dried milk).
The form of the leavenBiological activity "Picaflor" after storage for 1 month at 20°C (CFU/g)The biological activity of fermented milk product (CFU/ml)
porous tablet of example 15,6±0,4×1097.2 ą 0.3×108
porous tablet according to example 22,8±0,4×1095,8±0,3×108
porous tablet of example 32,1±0,4×1095,0±0,3×108
porous tablet of example 40,6±0,4×1094.2 ą 0.3×108
powder2,2±0,3×1091.4±0.1×108

Example 7. Composition "Picaflor-P, obtained in the conditions of example 1 were stored at 37°C for 2 weeks, after it was determined the biological activity of the dry composition. Then dry composition was recitatives by adding 60 ml of drinking water and cultivation is up in thermostat at 37°C for 18 hours. In conclusion, the experience was defined biological activity of the obtained fermented milk product. For comparison, in the same conditions of temperature stored samples, where the inoculum was used tablets yeast obtained according to the technology of examples 2 and 4, and the crushed product "Picaflor" (control). The percentage ratio between the components: feed / seed product "Picaflor-P was similar for control and experimental samples. The results are given in table 2.

Table 2.
The influence of the shape of the leaven after storage conditions on the properties of fermented milk product (original composition: 0.26 g of yeast probiotic; 12.5 g of the nutrient medium is crushed dried milk).
Probiotic "Picaflor" (leaven),Biological activity "Picaflor-P" after storage for 2 weeks at 37°C (CFU/g)The biological activity of fermented milk product (CFU/ml)
porous tablet of example 13,1±0,3×1094,6±0,3×108
then the flock tablet according to example 2 2,2±0,3×1091,4±0,3×108
porous tablet of example 41,1±0,3×1090,4±0,3×108
powder0,01±0,002×1095,1±0,4×104

The results showed that the proposed technology allows to preserve the product for a long time and get quality milk product with the desired probiotic properties (biological activity) in a relatively simple technologies suitable for use in conditions of high positive temperatures during long-term space flight or Autonomous underwater navigation.

Example 8.

Getting the leaven. In a vial with liofilizovane standard production strain Lactobacillus helveticus make 1 ml of distilled water, and then mix thoroughly, put in the test tube with skim milk and incubated at 37°C for 16-20 hours. The seed material is injected into a flask containing 100 ml hydrolysate-dairy medium containing pancreatic hydrolysate of milk 0.2 g of peptone, 0.06 g of sodium chloride, 0.002 g of cysteine and 0.3 g of lactose and incubated at 37°C for 18-20 hours Verisimilitudinous culture is on the environment, containing 1 l of pancreatic hydrolysate of milk, 2 g peptone, 0.6 g of sodium chloride, 0.02 g of cysteine, 4 g of calcium carbonate and 3 g of lactose at 37°C for 18-20 hours. To the culture fluid type 4-component protective environment consisting of sucrose, gelatin, skim milk powder (OSM) and ascorbic acid 10%: 2%: 4%: 5% (% mass.) calculated on dry substance of the final product

The resulting product is poured into aluminum forms 2.0±0.05 ml in each, what is the thickness of the dried layer 6 mm and freeze in the chamber of the pilot freeze-install alpha 1-20 ("chryst", Germany) at minus 30-35°C for 1.5 hours, and then subjected to freeze-drying under represented in figure 2 thermogram. The temperature of the condenser-memoratives during the whole drying process is minus 50°C-55°C and working pressure in the freeze-drying chamber is 13-15 PA. The time to reach the working vacuum is 20-30 minutes, the total duration of the drying process - 48-50 hours.

Thermogram drying of Lactobacillus helveticus in the process of production of porous tablets based on them similar to those shown in figure 2.

The biological activity of the tablets in the case of Lactobacillus helveticus is at least 6.2×108CFU/tablet.

When cooking dry 2-component product COI is lovelas the following ratio of components: freeze-dried powdered milk 12.5 g; obtained according to the technology of sublimation forming a porous tablet of the probiotic on the basis of Lactobacillus helveticus is 0.260, the Obtained sample dry nutrient medium (freeze-dried and ground drinking milk brand "Full pot" fat 1,5%) packaged in sealed containers. There was placed a porous tablets of Lactobacillus helveticus (1 tablet each), after which the vessel was germetizirovany.

To obtain a fermented probiotic drink containing L.helveticus in a container of dry product was added 60 ml of drinking water. Capacity with rehydration material was covered and placed in a thermostat heated to 37±2°C for 20 hours By lapse of time of exposure, the obtained milk product was removed from thermostat and checked for the presence of probiotic properties: biological activity, antibiotic resistance, antagonism against pathogenic and conditionally pathogenic microorganisms, kislotoobrazovanie.

The biological activity of the obtained fermented milk product on the basis of L.helveticus amounted to 5.2×108CFU/ml, the pH of the obtained product was equal to 4.4. The antibiotic resistance and antagonism is consistent with the rate declared for probiotic "Picaflor".

As follows from the above examples, the experimental results showed that tawlae the traveler technology allows to produce high-quality milk product with the desired probiotic properties (biological activity) by a simpler manufacturing techniques, suitable for use in conditions of high positive temperatures during long-term space flight and Autonomous underwater navigation.

1. Dry probiotic active biological product, including leaven "Vilaflor and milk-containing component, characterized in that it contains a ferment in the form of porous tablets with a pore size of at least 50% of its volume, the receipt of which includes: filling aluminum forms, cooling to a temperature of minus 35 to 45°C. the layer thickness of 6-7 mm, followed by exposure at a temperature of minus 30-35°C and freeze-drying at a temperature of condenser-memoratives not higher than minus 50°C and the pressure in the working chamber 13 and 15 PA for 48-50 hours, the time sublimation crystallized moisture is 20 hours at a temperature of the material is not above 30°C, and final drying is carried out for 8 h at 25°C.

2. Dry probiotic active biological product according to claim 1, characterized in that the leaven contains 5-10 wt.% of ascorbic acid.

3. Dry probiotic active biological product according to claim 1, characterized in that the tablet has a diameter 19,0±1.0 mm and a height of 6.5±0.5 mm



 

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4 cl, 4 ex

FIELD: food industry.

SUBSTANCE: invention relates to food industry. A water-dispersible granulate has average weighted weight diameter at least 100 mcm, preferably at least from 200 mcm and up to 2000 mcm. The said granulate contains: 40-90 wt % of carbohydrates; 2-30 wt % of lipids containing unsaturated fatty acids in an amount of at least 1%, preferably no less than 3% of the granulate weight and at least 30 mg/kg - 800 mg/kg of a metal enhancing oxidation and selected from the group consisting of ferrum, cuprum and their combinations wherein at least 50 wt %, preferably at least 70 wt % of lipids are present in the form of undispersed lipids.

EFFECT: invention allows to produce a granulate resistant to oxidation the production method whereof is implemented under mild conditions.

18 cl, 6 tbl, 3 ex

FIELD: process engineering.

SUBSTANCE: proposed invention may be used in production of semolina from animal feed by wet granulation technology. Proposed method comprises granulation of loose animal feed with addition of liquid component into press granulator, drying and cooling of granulated animal feed, crushing of obtained product and its fractionating into three fractions: large, medium and fine fractions. It includes also feeding large fraction for crusging and discharge of medium fraction as finished product. Said liquid component represents phototrophic biomass of micro seaweed added to animal feed in mixing in amount of 10…15%. Granulated animal feed is air dried at 70…80°C and with air flow rate of 2300…2700 m3/(h·t), and cooled by air at 7…10°C and with air low rate of 1200…1400 m3/(h·t). Medium fraction, prior to discharging it in the form of finished product, is dragged by vegetable oil fusa in amount of 10…12 wt % of medium fraction weight. Vegetable oil fusa is preheated to 60…70°C, enriched by anti-oxidising agents and fat-soluble ferments and filtered. Then obtained product is coated by fine fraction. Finished semolina is kept in ventilated hopper. Absorption refrigerator is used for recovery of cooling and hot air, as well as for heating water used for heating vegetable oil fusa. Proposed production line comprises animal feed hopper, press granulator, cooling column divided into drying and cooling zones, toll mill and two-sieve screening machine. Additionally, production line comprises site for seaweed growing, dragging site, vegetable oil fusa preparation site, finished semolina storage and absorption refrigerator.

EFFECT: expanded range of animal feed, increased biological value.

2 cl, 1 dwg

FIELD: food industry.

SUBSTANCE: treated food product production method envisages compressed air injection into a housing. The housing contains a section in the shape of frustum of cone. The injected air moves downwards through the housing and, having reached the lower end of the housing, flows back upwards and is released from the housing through an outlet hole. The treated food product with a water content of less then 14% (weight) and a lipid content of more then 15% (weight) is injected into the housing, captured by the injected air and milled. Then obtained granulated product is discharged from the lower end of the housing and mixed at least with one other ingredient of the treated food product. Then the treated food product is prepared from them.

EFFECT: invention allows to process the treated food product with a low moisture and a high fat lipids content, to implement a short-duration operation of granulation at one device without moving mechanical parts usage and to use the produced granulated product during treated food products production.

18 cl, 4 dwg, 2 ex, 1 tbl

FIELD: food industry.

SUBSTANCE: soya bean seeds are washed and wetted in water whereupon protein substances are crumbled, extracted and suspended in water. Further lacto serum is added into suspension as coagulant at ratio 1:1. Suspension is divided into solid and liquid fractions. Moisture contents of solid fraction is brought to 25-30%. Produced mass is granulated with dimension of granule diametre 1.0-2.0 mm. Further there is performed two-stage drying at temperature 110°C during 30 minutes and at temperature 180°C till appearance of specific golden-yellow colour. Also produced granules can be crumbled to a powder state.

EFFECT: invention facilitates production of natural food product containing vegetable and animal proteins, fats, adequate amount of carbohydrates and mineral substances with good organoleptic indices at relatively low cost due to implementing inexpensive components of its composition with long storage life increasing terms of selling and utilisation.

2 cl, 1 tbl, 2 ex

FIELD: food products.

SUBSTANCE: agglomerated food thickener contains pregelatinised starch and water-soluble covering agent. The agent contains substance chosen from the group including gum arabic, gum arabic treated with 1-oktenyl succinic anhydride, starches treated with 1-oktenyl succinic anhydride, maltodextrins treated with 1-oktenyl succinic anhydride, dextrins treated with 1-oktenyl succinic anhydride and mixtures of two or more of the above.

EFFECT: stable starch processed for its fast dispersibility both in hot and cold water.

20 cl, 2 dwg, 3 tbl, 4 ex

FIELD: food products, canned food production.

SUBSTANCE: invention relates to food-concentrates industry, namely to manufacturing of potato-based instant products. The dry mix contains potato product, protein component and food additives. Potato flakes are used as potato product, dry vegetable cream - as protein component, food additives are fat powder "Vekokrim", sodium glutamate, ribotides, tomato powder, salt, sugar, and additionally - ground black pepper, ground red pepper, dried bunching onion, dried parsley and broken onion with definite components proportion. The instant product contains the above mix for potato puree production and vegetable or meat-and-vegetable canned food with definite components proportion. The invention allows reaching acceptable consumer consistency of end product, well-balanced composition and optimal organoleptic properties.

EFFECT: manufacturing of a product with increased digestibility and optimal organoleptic properties.

4 cl, 2 ex

FIELD: the invention refers to equipment for extrusion processing of mixed fodder on mixed fodder plants and also for production of extrusion food products in different fields of food industry.

SUBSTANCE: the extruder's forming head has a body, a flange connected to it, a calibrating matrix, a shaft connected with a screw and a knife. A chamber with sockets for removal water steams and unloading ready product is fastened to a matrix. Inside the chamber there is a perforated conical drum. Vertical holders are installed on the shaft. On the spherical ends of the vertical holders there are fixed sectional strings. The strings are fulfilled with possibility of turning them relatively to the shaft with following fixation with bolts for measuring of a lead angle. On the external surface of the strings there are longitudinal notches.

EFFECT: reduces power inputs on production of well-milled extruders.

4 dwg

FIELD: food-processing industry.

SUBSTANCE: method involves preliminarily cutting, pressing and granulating raw material, with juice being expelled at grinding and pressing stages; drying resulted granules by providing infrared power feeding from each side, with initial content of dry substances in pumpkin granules constituting 16%, diameter of granules making 0.004 m, and heat flux density making 2.69 kW/m2.

EFFECT: increased yield of dry product, increased nutrient value and content of vitamins.

2 tbl, 3 ex

FIELD: food-processing industry.

SUBSTANCE: solid bouillon tablet contains, wt%: from 1 wt% to 20 wt% of oil and, possibly, up to 80 wt% of fat; preferably from 4 wt% to 80 wt% of crushed filler; up to 95 wt% of non-crushed filler; from 4 wt% to 35 wt%, preferably from 4 wt% to 20 wt%, of binding agent, and % by total weight of oil and fat, up to 40 wt% or even up to 30 wt%, preferably up to 20 wt% or even up to 10 wt%, or, which is more preferable, only up to 5 wt%, or even only 1 wt% of fat. Method involves preparing dry premix from powdered components, said premix containing crushed and non-crushed filler; spraying oil and, possibly, fat onto prepared premix; mixing resultant premix and adding dried vegetables and/or spicy leaves; thereafter, producing tablets from resultant mixture.

EFFECT: improved quality of solid bouillon tablet.

10 cl, 3 ex

FIELD: biotechnology.

SUBSTANCE: invention relates to a strain of the bacteria Lactobacillus plantarum Inducia DSM 21379 used as a probiotic. The strain enhances the natural protective potential of organism, produces polyamines from ornithine and glutamate, nitric monoxide (NO) from arginine, possesses antioxidative activity and enhances the intestinal barrier function, stimulating cellular immunity of the intestinal mucosa and blood, and stimulates adaptively the proinflammatory cytokines. The strain in an amount providing an effective daily dose of from 108 to 109 CFU/ml or CFU/g is used for preparing a composition for use as an antioxidant probiotic.

EFFECT: increased cellular immunity.

7 cl, 8 dwg, 14 tbl, 6 ex

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