Agent for antiplatelet, anti-inflammatory and cytoprotective therapy

FIELD: medicine, pharmaceutics.

SUBSTANCE: invention refers to medicine, and concerns an agent for prevention and treatment of cardiovascular diseases. The peptide 3-mercaptopropionyl-Phe-Ile-lle-Arg-Lys-Pro-Asp-Lys-NH2 is synthetised which has greater availability and lower price than known analogues; it possesses the antiplatelet, anti-inflammatory and cytoprotective properties.

EFFECT: invention provides reduced spontaneous thrombocyte aggregation, inflammation inhibition by reducing histamine secretion, cell protection against neurotoxic actions of thrombin.

1 cl, 4 ex


The present invention relates to medicine, in particular to the means for prevention and treatment of diseases of the cardiovascular system.

One of the causes of mortality in patients with various cardiovascular diseases and acute cerebrovascular disease (cerebral vascular accident) are thrombotic complications. The most important participants in the development of such complications are platelet activation, which plays a key role in the launch of the mechanisms of thrombus formation. It is known that platelets in pathology acquire the ability to spontaneously aggregate to form aggregates of small size, which can circulate in the blood of patients and to be the centers that in areas of damaged endothelium formed clots. The formation of such units depends on a number of reasons, the most important of which are inflammation and the initiation of the formation of thrombin, which leads to increased expression of cells of the cardiovascular, immune and nervous system receptors, thrombin and other proteases, called receptors, activated proteases (PAIRS).

There are four subtypes PAR: PAR-1, PAR-2, PAR-3 and PAR-4. According to the latest data, the blockade by antagonists of PAIRS of functions of these receptors inhibits platelet activation induced by thrombin and block the processes of inflammation and killed the Lee of cells (apoptosis), including neurons. In humans is mediated by thrombin activation of platelets occurs through PAR-1 and PAR-4, therefore, the search and selection of antagonists PAIRS directed to the development of drugs capable of inhibiting these receptors. The most important PAR-1 is the major thrombin receptor, mediating events associated with inflammation and activation of blood coagulation. The specificity of binding of PAR-1 by thrombin almost 2 orders of magnitude higher than that of PAR-3 and PAR-4, so PAIRS of 4 persons is activated by thrombin in higher concentrations than PAR-1.

Inflammation and the initiation of the formation of thrombin is one of the reasons for the development of atherogenesis and its complications - atherothrombosis, cerebral vascular accident (stroke), etc. Should be noted that the use of known drugs aspirin combined with clopidogrel or the combination of aspirin with dipyridamole - to reduce blood clots and exclusion of recurrent stroke in patients with ischaemic stroke of arterial origin leads to side effects (bleeding and disorders of the digestive system).

Development of new and highly effective antiaggregatory, anti-inflammatory and cytoprotective therapy at the present time is very important.

In the international application WO/2004/098628, C07K 5/09 serves to suppress activation of the aggregation process, trombi is tov to use synthetic analogs of the peptide Arg-Pro-Pro-Gly-Phe, which contain one or more amino acid substitutions. A disadvantage of the proposed peptide analogues is that they are direct inhibitors of a molecule of thrombin. They block proteolytic cleavage by thrombin all its substrates (including amide and protein) and thus, slows platelet aggregation, while significantly prolong the clotting time of blood, which is a negative side effect.

In [Andrade-Gordon P. A. O. PNAS. 1999; v.96(22): R-12262 and Derian S.K., etc. Biochemistry 2002, t, p.66-76] was synthesized and tested as antagonist PAR-1 peptidomimetic RWJ-56110 the following structure:

RWJ-56110 - [(S)-N-[(1S)-3-amino-1-[[(phenylmethyl)amino)carbonyl]-[[[[1-(2,6-dichlorophenyl)methyl]-3-(1-pyrrolidinyl)-1H-indol-6-yl]amino]carbonyl]amino]-3,4-diftorbenzofenonom.

However, RWJ-56110 inhibits aggregation induced only very low concentrations of thrombin, and when they increase insolvent, which limits its applicability. In addition, the synthesis of these compounds is complex.

[Morley D. S.A. Pharm. Rev. 2002, v.54: p.203] was synthesized peptide 3-mercaptopropionyl-Phe-Cha-Cha-Arg-Lys-Pro-Asp-Lys-NH2that exhibits activity as an inhibitor of PAR-1. As can be seen from the above structure, the sequence of this peptide contains the it two residue β-cyclohexylamine (Cha). It should be noted that the derivative of this amino acid is expensive: the cost required for the synthesis of a derivative of this amino acid in 18 times higher than the cost of similar bifunctional derivatives of natural amino acids. Thus the disadvantage of peptide is its high cost and low availability.

Object of the present invention to provide an effective means antiaggregatory, anti-inflammatory and cytoprotective therapy in the form of a peptide antagonist PAIRS 1, wherein a lower cost and affordability in comparison with the known analogues.

The goal was solved by the synthesis of a peptide of the following structure: S-mercaptopropionyl-Phe-Ile-Ile-Arg-Lys-Pro-Asp-Lys-NHz (IPAR-1-I).

It was unexpectedly found that the inclusion in the structure of the peptide instead of two residues of β-cyclohexylamine, residues isoleucine allows you to get a connection with antiaggregatory, anti-inflammatory and cytoprotective activity and at the same time much cheaper than the peptide-analogue. Tests IPAR-1-I ex vivo showed that the use of IPAR-1-I allows you 3 times to reduce the degree of spontaneous platelet aggregation (SPA) patients with coronary heart disease (CHD). IPAR-1-I also has anti-inflammatory and cytoprotective activity.

The following examples illustrate comprises the acts invention.

Example 1.

Obtaining peptide 3-mercaptopropionyl-Phe-Ile-Ile-Arg-Lys-Pro-Asp-Lys-NH2(IPAR-I).

For solid-phase peptide synthesis (TPS) used a derivative of the amino acid L-series. The peptide was synthesized using Fmoc methodology, i.e. in combination with the Nα-Fmoc-protected to block the side chains of the amino acids used kislotolabilen protective groups: BOC - ε-amino groups of lysine, But-β-carboxyl function aspartic acid, Trt - for carboxamide function of asparagine and SH-groups mercaptopropionic acid, as well as Pmc for guanidino function of arginine. The synthesis was carried out on an insoluble polymer carrier is a copolymer of styrene with 1% divinylbenzene with anchor group, designed to obtain amides of the peptide - Rink polymer containing amino groups 0.64 mmol/g Synthesis of the peptide was performed on the basis of 0.2 g (0.125 mmol) of the polymer, since the C-end and increasing peptide chain by one amino acid. For removal of Nα-Fmoc-protection was used a 25% solution of 4-methylpiperidine to create amide bond used TBTU/HOBt in the presence of diisopropylethylamine. For completeness of the reactions in the process of TFS used test Kaiser. At the end of the TFS of the target product was tsalala from polymer with simultaneous removal of all protective groups of the side chains of amino acids, trifluoroacetic acid is you. The obtained crude peptide IPAR-1 was purified using preparative HPLC. The peptide is characterized by the data of NMR spectroscopy and mass spectrometry. In the mass spectrum of this substance was present molecular ion corresponding to the correct molecular weight of the target product (M=1217,53). Range1H-NMR confirms the structure of the synthesized peptide.

Example 2.

Test antiaggregative actions peptide IPAR-I.

The test of the obtained peptide was performed using the blood of patients with coronary heart disease CHD, verified using coronary angiography. The blood of patients with coronary artery disease received by gravity from the cubital vein into the tube from 0.13 M solution of sodium citrate (pH of 7.3). After 15 min after sampling and transportation at room temperature, the blood was centrifuged to obtain platelet-rich plasma (OTP). Evaluation of the aggregation capacity of platelets in the OTP was carried out on a laser aggregometry NPF "Biola" (Russia), allowing to estimate the spontaneous formation of aggregates (which corresponds to the curve on the testimony of the average size of the aggregates, expressed in relative units), and formation of aggregates in response to high concentrations of inducers (curves, the registration process of transmittance, expressed in %). Platelet-rich plasma to write the aggregation process Incubus is listed for 5 min at room temperature with the appropriate IPAR. As a selective agonist of PAR-1 (to start the mechanism of spontaneous aggregation) used the peptide agonist PAIRS-1 - SFLLRN.

The study antiaggregative actions IPAR - 1-I showed that IPAR - 1-I reduces spontaneous platelet aggregation CHD patient with a high value of this indicator almost to normal values. Spontaneous platelet aggregation was decreased in 3 times.

Example 3.

Test anti-inflammatory actions of peptide IPAR-I.

Among the cells involved in the inflammatory responses of the body, an important role belongs to the fat cells. Mast cells Express a variety of receptors, including the receptors of the family of PAIRS. The presence of fat cells receptor PAIRS is responsible for their reactivity against thrombin. Activated mast cells release a wide range of proinflammatory mediators, including histamine. Methods of evaluation of anti-inflammatory actions IPAR-I was based on measuring the secretion of mast cell histamine in the presence of IPAR-1-I.

Fat cells are taken from white male outbred rats weighing 250-350 g Acute inflammation in rats caused by intraperitoneal injection of thioglycolate Na. Fat cells were separated by centrifugation peritoneal fluid, suspended in HEPES-NaCl and purified in the gradient ficoll. Histamine secreted by fat cells, was determined with POM is by its condensation with ortho-phthalaldehyde, resulting in a fluorescent complex. Fluorescence was measured on the spot an Thermo Fluoroscan Ascent at 460 nm by exciting at 355 nm.

Study of anti-inflammatory actions IPAR-I, respectively, inhibiting them from secreting mast cells histamine showed that IPAR-I could inhibit inflammation, significantly reducing the secretion of histamine.

Example 4.

Test cytoprotective effect of peptide IPAR-I.

Studies were performed on primary cultures of hippocampal neurons (9-10 days) brain one to three-day rat Wistar rats. The obtained cell suspension was transferred on cover glasses coated with poly-D-lysine. Then deleted neprecejusies cell was added in the culture medium. 3-4 day arabinoside was added on day to inhibit the growth of glial cells. The cells were left for 7-10 days in the incubator. Replace 1/3 of the environment in the cells was carried out every 3 days. The obtained culture contained no more than 5% of glial cells.

Death/survival of neurons in culture was determined 24 hours after the 45-minute action of thrombin or after co-incubation IPAR-I with thrombin. Peptide IPAR-I (in concentrations from 0.4 to 100 nm/l) was treated neurons for 10 min before the addition of thrombin. As a control, neurons were treated with HEPES-saline buffer (HBSS). Death/viive the ity of neurons was assessed by biochemical MTT (3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide) method. An aqueous solution of MTT was added to the culture medium to a final concentration of 1 mg/ml, and incubated the cells for 1.5 hours at 37°C. Then the medium was collected and DMSO was added to dissolve formisano. The optical density was measured spectrophotometrically (590 nm) at universal microplate spectrophotometer Anthos Lucy 1. Estimated percentage compared to control.

It was found that thrombin at a concentration of 100 nm/l caused the death of more than 20% of neurons (reduced survival relative to the control). The incubation of cells with IPAR-I before adding thrombin protected them almost completely from the neurotoxic action of thrombin (level of living cells compared to the control values were 98%).

Thus synthesized peptide has antiagregatini, anti-inflammatory and cytoprotective activity in greater availability compared with the nearest equivalent.

Peptide 3-mercaptopropionyl-Phe-Ile-Ile-Arg-Lys-Pro-Asp-Lys-NH2as a means antiaggregatory, anti-inflammatory and cytoprotective therapy.


Same patents:

FIELD: medicine, pharmaceutics.

SUBSTANCE: there are presented 3-(2',2'-dimethylpropanoylamino)-tetrahydropyridin-2-one , (S)-3-(2',2'-dimethylpropanoylamino)-tetrahydropyridin-2-one and pharmaceutical compositions prepared with using said compound or its isomer, as well as the use thereof for preparing a therapeutic agent for preventing or treating inflammatory conditions, and a related method of treating. What is shown is the advantage of the compounds as related to the other broad spectrum chemokine inhibitors (BSCI) on anti-inflammatory activity; it possesses the pharmacokinetic, toxicological properties and the pharmaceutical safety parameters: (S)-3-(2',2'-dimethylpropanoylamino)-tetra-hydropyridin-2-one is 5-25 times more active than (R)-isomer.

EFFECT: higher anti-inflammatory activity.

13 cl, 10 dwg, 9 tbl

FIELD: medicine, pharmaceutics.

SUBSTANCE: invention relates to combined antipyretic drug in form of rectal suppositories. Drug contains paracetamol, dimedrol and papaverine hydrochloride as active components, and lipophilic suppository base Suppocire (Suppocire NA-15) as auxiliary substance, with the following component ratio in g per 1 suppository with 2.0 g weight: paracetamol 0.3-0.5; dimedrol 0.03-0.05; papaverine hydrochloride 0.03-0.05; Suppocire (Suppocire NA-15) - the remaining part (to 2.0 g).

EFFECT: increased efficiency of paracetamol suppositories as antipyretic drugs, with absence of narcotic and psychostimulating substances in their composition.

4 tbl, 2 ex

FIELD: medicine, pharmaceutics.

SUBSTANCE: present invention refers to new compounds of general formula I [X]n-Y-ZR1R2, wherein the radicals are specified in the description, effective as heparan sulphate-binding protein inhibitors. The invention also refers to a pharmaceutical or veterinary composition having heparan sulphate-binding protein inhibitory activity for preventing or treating a disorder in a mammal, and to the use of these compounds and compositions for antiangiogenic, antimetastatic, anti-inflammatory, antimicrobial, anticoagulant and/or antithrombotic therapy in a mammal.

EFFECT: preparing the new compounds of general formula I [X]n-Y-ZR1R2, wherein the radicals are specified in the description, effective as the heparan sulphate binding protein inhibitors.

10 cl, 31 ex, 11 tbl, 40 dwg

FIELD: medicine, pharmaceutics.

SUBSTANCE: present invention refers to an aminopropylidene derivative presented by formula wherein R1 and R2, which may be identical or different, represent hydrogen or a substitute specified in the following (a)-(c), provided the case of both representing hydrogen is excluded: (a) carbonyl substituted with hydroxy, alkoxy or hydroxy alkylamino, (b) carbonylalkyl substituted by hydroxy or alkoxy, and (c) acrylic acid including its alkyl ester, R3 and R4, which may be identical or different, represent hydrogen, alkyl which may be substituted by phenyl or cycloalkyl, or R3 and R4, which together form a heterocyclic ring with a nitrogen atom bound thereto, represent pyrrolidino, piperidino, which may be substituted by oxo or piperidino, piperazinyl substituted by alkyl or penyl, morpholino or thiomorpholino; A means oxo or is absento, B represents canbon or oxygen; one of X and Y represents carbon, while the other one represents sulphur, a part represented by a dash line represents a single bond or a double bond, and a wavy line represents a cys-form and/or a transform. Also, the invention refers to a pharmaceutical composition exhibiting histamine receptor antagonist activity on the basis of said compounds.

EFFECT: there are produced new compounds and pharmaceutical compositions thereof, which can be used in medicine for treating asthma, allergic rhinitis, pollen allergy, hives and atopic dermatitis.

10 cl, 12 tbl, 58 ex

FIELD: medicine, pharmaceutics.

SUBSTANCE: invention refers to medicine, and concerns a pharmaceutical composition for treating skin diseases. The composition contains a combination of methylprednisolone aceponate and glycoceramides and a glycoceramide complex containing cholesterol in the amount of 1-3%, and phospholipids in the amount of 25-34%, and excipients. A method for preparing the composition consists in the fact that methylprednisolone aceponate is introduced into an emulsion base containing the glycoceramide complex in the form of a solution to form a coagulation structure.

EFFECT: new pharmaceutical composition is characterised by a wide spectrum of pharmacological properties, stability, uniform distribution of the active ingredients.

9 cl, 2 tbl

FIELD: medicine, pharmaceutics.

SUBSTANCE: present invention refers to a compound of formula (I) presented below wherein the radicals and symbols have the values presented in the patent claim, and/or to its racemate, enantiomer, diastereomers or its pharmaceutically acceptable salts and/or esters. The invention also refers to a method for preparing it, using it in preparing a drug preparation and to drug preparations containing the compound of formula (I).

EFFECT: compound of formula has analgesic action and may be used as an active compound for pain management.

20 cl, 3 tbl, 33 ex

FIELD: medicine, pharmaceutics.

SUBSTANCE: invention refers to a compound having formula (I): wherein each of the groups R1, R2, R3 are independently H or C1-4 alkyl group or C2-4 acyl group; each of the groups R4 and R5 are independently H or the group of formula -SO3R6, wherein R6 is H or the C1-4 alkyl group or the C2-4 acyl group; provided at least one of the groups R4 and R5 is a group having formula -SO3R6, or a pharmaceutically acceptable salt thereof. The invention also refers to a method for preparing the specified compounds of formula (I) and a pharmaceutical composition possessing activity in the inhibition of IL-1 anti-inflammatory cytokines based on these compounds.

EFFECT: there are produced new compounds and pharmaceutical composition on their basis which can find application in medicine for treating an inflammatory or autoimmune condition.

17 cl, 4 dwg, 4 ex

FIELD: medicine, pharmaceutics.

SUBSTANCE: invention relates to field of biotechnology and immunology. Claimed is medication for treatment and prevention of TNF-dependent disorder, which contains molecule of TNF-binding nanobody, lyoprotector, surface-active substance and buffer, method of such medication obtaining, method of lyophilised preparation reduction and method of analysis of medication-manufacturing process, as well as method and set for treatment or prevention of TNF-dependent disorder.

EFFECT: invention ensures obtaining stable pharmaceutical preparations of TNF-binding nanobodies and can be applied in therapy of TNF-dependent diseases.

30 cl, 12 ex, 3 tbl, 32 dwg

FIELD: medicine, pharmaceutics.

SUBSTANCE: invention refers to compounds of formula I , wherein R2 means methyl, Y means carbon or nitrogen, and R1, R3 and R4 have the value specified in the patent claim. Also, the invention refers to a pharmaceutical composition for the use as a pharmaceutical drug having activity of a phosphatidylinositol-3-kinase inhibitor, to the use of the compounds of formula I for preparing the pharmaceutical drug for treating a disease mediated by phosphatidylinositol 3-kinase and to a method for preparing the compounds of formula I .

EFFECT: preparing the compounds of formula I possessing activity of the phosphatidylinositol-3-kinase inhibitor.

10 cl, 5 tbl, 51 ex

FIELD: medicine, pharmaceutics.

SUBSTANCE: present invention refers to a method of intraocular pressure reduction and to a method of pain control involving the administration of a therapeutic compound representing , or its tautomer or stereoisomer forms wherein X represents NH; n is equal to 2 or 3; Ra, Rb, Rc and Rd represent stable functional groups independently consisting of: 0 to 4 carbon atoms, 1 to 9 hydrogen atoms; and Re represents H or C1-4alkyl. Furthermore, the invention refers to a compound represented by formula , or to its tautomer or stereoisomer form.

EFFECT: new compound is prepared; besides, the known compounds to be applied in pain and glaucoma control are studied.

8 cl, 1 tbl

FIELD: medicine, pharmaceutics.

SUBSTANCE: invention refers to pharmaceutical industry and represents a pharmaceutical composition for treating and preventing viral and bacterial infections, containing as active ingredients: lysocyme, peroxidase, poviargol, as anti-inflammatory ingredients: escin and glycyrrhizic acid or a salt thereof, as carriers - liposome based on high-active hydrogenated lecithins in a combination with cholesterol and pharmaceutically acceptable carriers and excipients, and the ingredients of the composition are taken in a certain ratio, wt %.

EFFECT: invention ensures maintaining prolonged activity of the enzymes being the ingredients of the composition, fast skin penetration and absorption.

4 ex, 1 tbl

FIELD: medicine.

SUBSTANCE: invention relates to medicine, namely pulmonology, and can be used for treatment of patients with chronic obstructive lung disease. For this purpose, complex therapy, which includes carrying out nebuliser inhalations of lysocium and gas-air carbon dioxide baths at the background of symptomatic drug therapy and respiration gymnastics, is realised to patient. In order to carry out nebuliser inhalations ofr lysocium, content of lysocium bottle - 0.05 g is preliminarily dissolved in 10 ml of isotonic sodium chloride solution. 4 ml of lysocim solution are poured into nebuliser chamber. Inhalations are performed for 7-9 minutes, daily, 10-12 procedures per course. Then, after 30-60 minutes gas-air carbon dioxide baths with temperature of gas mixture 36°C are carried out, exposure time is 20-25 minutes, daily, 12-15 procedures per course. Mixture supply is realised for 6-7 minutes at rate 50 l/min. carbon dioxide concentration constitutes 43-48%.

EFFECT: method ensures increase of treatment efficiency due to expressed anti-inflammatory and broncholytic action, improvement of humoral immunity and gas-exchange function of lungs with reduction of drug load on organism.

2 ex

FIELD: medicine, pharmaceutics.

SUBSTANCE: invention refers to biology and medicine, and concerns a lifespan increasing drug.

EFFECT: substance of the invention involves the use of hyaluronidase immobilised by electron-beam synthesis nanotechnology as a drug for retardation of natural aging and lifespan increase.

1 ex, 3 tbl

FIELD: medicine, pharmaceutics.

SUBSTANCE: invention refers to pharmacology and medicine, and represents a composition for treating and preventing a disorder related to digestive enzyme insufficiency, containing a set of coated particles; each said particle comprises a enteric-coated core wherein the core contains pancrelipase and the enteric coating contains 10-20 wt % of at least one enteric-coated polymer and 4-10 wt % of talc wherein said wt % are calculated from total weight of the coated particles and wherein the coated particles show pancrelipase enzymatic activity loss by no more than approximately 25% after 6 months of accelerated stability test.

EFFECT: invention provides enzyme activity stability.

47 cl, 14 ex, 35 tbl

FIELD: medicine, pharmaceutics.

SUBSTANCE: invention refers to pharmaceuticals and concerns a controlled release pharmaceutical composition containing an oral dosage form of pancreatine, and an enterosoluble coating which contains a) a film-forming agent; b) a plasticiser representing mixed cetyl alcohol and triethyl citrate, and c) optionally at least one adhesion-blocking agent. The invention also concerns a method for preparing said composition and applying it for producing a pharmaceutical for digestive disorders, pancreatic exocrine insufficiency, pancreatitis, mucoviscidosis, insulin-dependent and/or insulin-independent diabetes.

EFFECT: invention provides the composition having the improved release profile and storage stability.

11 cl, 4 tbl, 14 ex

FIELD: medicine, pharmaceutics.

SUBSTANCE: invention relates to biology and medicine. Hyaluronidase immobilised with using an electron-beam synthesis technology is applied as an agent for intensifying the action of biologically active substances and drugs of various pharmacological groups.

EFFECT: application of said hyaluronidase allows improving the efficacy of biologically active substances and drugs.

7 tbl, 6 ex

FIELD: medicine, pharmaceutics.

SUBSTANCE: invention refers to pharmacy, namely to a pharmaceutical composition for local application, containing enzyme hyaluronidase which can be used in pharmacy for treatment and prevention of inflammatory prostate diseases, such as prostatitis. The pharmaceutical composition for local application contains as an active ingredient hyaluronidase 2.44 wt %, Tween-80 2.93 wt %, PEG-1500 91.69 wt %, liposome 2.44 wt %, and pharmaceutically acceptable carriers or excipients, such as benzydamine 0.5 wt %.

EFFECT: invention provides treatment and prevention of inflammatory prostate diseases, such as prostatitis.

1 tbl, 2 ex

FIELD: medicine, pharmaceutics.

SUBSTANCE: invention refers to pharmacology and represents a method for making pancreatine including heating of a dispersed form of pancreatine containing one or more solvents at temperature at least 85°C for 48 h to ensure total concentration of solvents in the dispersed form of pancreatine equal or less than 3.5 wt % at any moment of said heating process, while the dispersed form of pancreatine is specified from powder, pellets, micropellets, microspheres, granules and granulates.

EFFECT: invention provides lower content of biologically active impurities in pancreatine and maintained enzymatic activity of lipase, protease and amylase at an acceptable level by 50 % and more from the initial enzymatic activity.

32 cl, 22 ex, 7 tbl, 5 dwg

FIELD: medicine, pharmaceutics.

SUBSTANCE: invention concerns gastroenterology and represents a composition for treatment of pancreatic insufficiency containing microbial lipase, microbial protease and microbial amylase, and the ratio of lipase, protease and amylase in the specified composition makes approximately 1:1:0.15 USP units.

EFFECT: invention provides preparation of stable compositions with pancreatic enzymes, exhibiting maximum efficiency with minimum dosages and characterised with a common safety profile.

31 cl, 3 ex, 10 tbl, 7 dwg

FIELD: medicine.

SUBSTANCE: intraperitoneal introduction to laboratory animals of hyaluronidase preparation in a dose of 1000 SU/kg once a day within 2 days is combined with subcutaneous introduction of granulocytic colony-stimulating factor (G-CSF) in a dose 125 mkg/kg once a day within 5 days.

EFFECT: effective myelopoiesis stimulation.

2 tbl, 2 ex

FIELD: medicine, pharmaceutics.

SUBSTANCE: invention refers to pharmaceutical industry, namely an agent possessing anti-inflammatory action. The agent possessing anti-inflammatory action contains as active ingredient in the form of a complex prepared of a coelomic fluid of sea urchins containing peptides and amino acids at a certain ratio of the ingredients.

EFFECT: agent possesses a manifested anti-inflammatory effect.

2 cl, 2 tbl, 10 ex