Method for prediction of left ventricular postinfarction dilatation
SUBSTANCE: patient's blood serum taken on the first three days following the myocardial infraction is examined for the level of a tissue inhibitor of type 1 metalloproteinase (TIMP-1) and the concentration of vascular cell adhesion molecules (sVCAM-1) to calculate a repair coefficient (RC) by formula: RC=TIMP 1 /sVCAM1, and if the CR values exceeds 0.94, the developing left ventricular postinfarction dilatation is predicted.
EFFECT: early prediction of the developing left ventricular postinfarction dilatation, including of the symptom-free forms.
3 ex, 1 tbl
The invention relates to medicine, namely cardiology, and can be used for assessment in the acute stage of myocardial infarction the risk of dilatation of the left ventricle during the first year after myocardial infarction.
In modern literature there is information about the use of natriuretic peptides (tap) atrial (PT) and brain-type (MT), and aminoanisole brain natriuretic propecia type (NT-proBNP) to confirm or refute the diagnosis of heart failure (Wu A.N., Apple F.S., Gibler W.., Jesse R.L., Warshaw M.M., Valdes R.Jr. National Academy of Clinical Biochemistry Standards of Laboratory Practice: recommendations for the use of cardiac markers in coronary artery diseases // Clin Chem. - 1999. - Vol.45. - P.1104-1121). However, it is shown that the concentration of natriuretic peptides in the blood is influenced by many factors, including age, sex, condition of the thyroid gland, anemia, and obesity. This leads to the complexity of the interpretation of the results should be interpreted only in the clinical context of related conditions. There is no reliable guidance on the use of natriuretic peptides to identify groups of individuals at high risk for development of post-infarction asymptomatic dilatation of the left ventricle, and, given the variety of pathogenetic factors leading to the expansion of the left heart chambers, N. triturations peptide does not reflect the specificity of postinfarction remodeling of the left ventricle. In the available literature we did not find ways to predict post-infarction left ventricle dilatation.
Technical result: early prediction of the development of post-infarction left ventricle dilatation, including asymptomatic forms.
The essence of the method consists in determining the serum of venous blood collected in the acute phase of myocardial infarction (within the first three days after a heart attack), the level of serum marker of metabolism of collagen, tissue inhibitor of matrix metalloproteinase-1 type (TIMP-1) and concentration of molecules intercellular adhesion to the vascular endothelium (sVCAM-1), followed by calculation of the coefficient repair (CR) by the formula CR=TIMP1/sVCAM1. The coefficient of reparation characterizes the balance between system collagenopathy and repair system. When the magnitude of the coefficient repair more than 0,94 predict the development of post-infarction left ventricle dilatation in the coming year developed after myocardial infarction.
The method is as follows.
In subjects with a diagnosis of acute myocardial infarction during the first three days after myocardial infarction take blood from the cubital vein in a volume of 10 ml without anticoagulant, defend for one hour, then centrifuged for 20 min with a speed of 2000 rpm and get savoro the ku blood. Then spend a solid phase enzyme-linked immunosorbent assay (ELISA) using a diagnostic kit company BenderMedSystem (Vienna, Austria) for the quantitative determination of tissue inhibitor of matrix metalloproteinases type 1 (TIMP-1) and molecules intercellular adhesion to the vascular endothelium (sVCAM-1). Calculate the coefficient of reparation (KR) according to the formula CR=TIMP1/sVCAM1. When the magnitude of the coefficient repair more than 0,94 predict the development of post-infarction left ventricle dilatation in the coming year installed after myocardial infarction.
The survey was attended by 39 people admitted to the intensive care unit with a diagnosis of acute myocardial infarction. All patients with acute myocardial infarction was diagnosed according to the criteria established by the recommendations of GFCF. Within three days after myocardial infarction all patients was determined by the concentration of TIMP-1 and sVCAM-1 in the serum of venous blood. The sizes of the chambers of the heart (end-diastolic volume of the left ventricle) investigated simultaneously with blood sampling and one year after discharge, respectively, LV EDV1and LV EDV2. 26 people through the observation year end-diastolic volume increased in 25 of them - the value of the coefficient of reparation exceeded 0,94.
Examples of specific performance:
Example 1. Female P., 73. He enrolled in the intensive care unit with the automotive technician is zoom acute myocardial infarction of posterior-lateral localization. On ultrasound of the heart during the acute stage of myocardial infarction end-diastolic volume of the left ventricle (LV EDV1) was 85 ml, in the serum level of TIMP-1 - 1335 ng/ml, the level of sVCAM-1 - 2040 ng/ml Coefficient of reparation was 0.65. One year after myocardial infarction by echocardiography revealed a decrease in LV EDV2to 55 ml, i.e. post dilatation has not developed.
Example 2. Man, 55 years. He enrolled in the intensive care unit with a diagnosis of acute anterior septal involving the tops of myocardial infarction. On ultrasound of the heart during the acute stage of myocardial infarction, LV EDV1- 63 ml, in the serum level of TIMP-1 - 1390 ng/ml, the level of sVCAM-1 - 585 ng/ml Coefficient of reparation was 2,38. One year after myocardial infarction by echocardiography revealed an increase in LV EDV2up to 105 ml, i.e. the development of post-infarction left ventricle dilatation.
Example 3: a Man F., 70 years. He enrolled in the intensive care unit with a diagnosis of acute myocardial infarction of posterior-lateral localization. On ultrasound of the heart during the acute stage of myocardial infarction, LV EDV1158 ml, in the serum level of TIMP-1 - 1870 ng/ml, the level of sVCAM-1 - 1770 ng/ml Coefficient of reparation was 1.06. One year after myocardial infarction by echocardiography revealed an increase in LV EDV2to 165 ml, i.e. the development of post-infarction left ventricle dilatation.
The sequence number of the patient BWW LV2 (a year)- LV EDV1 (AMI)(ml) The coefficient of reparation (TIMP-1/sVCAM-1) 1 -2 1,03 2 -30 0,65 3 15 1,79 4 -24 0,91 5 7 1,06 6 -44 0,55 7 16 1,03 8 -15 0,73 9 -27 0,49 10 -3 0,04 11 35 1,26 12 -11 13 12 1,7 14 6 1,01 15 19 1,04 16 6 0,96 17 2 of 1.34 18 9 1,30 19 -6 0,62 20 8 1,18 21 19 1,75 22 -13 0,71 23 22 1,59 24 8 1,91 25 -23 0,78 26 5 1,82
27 23 0,90 28 42 2,38 29 14 1,43 30 -6 0,39 31 -9 0,94 32 10 1,93 33 45 1,39 34 55 1,18 35 15 of 1.57 36 24 0,81 37 63 0,98 38 25 2 39 75 0,94
Pros: the simplicity of the method, the possibility of detection with a sensitivity of 92% and the specificity 92%, among persons with acute myocardial infarction, high risk asymptomatic post-infarction left ventricle dilatation in the course of the next year to develop therapeutic approaches and strategies to reduce the risk.
A method for predicting post-infarction left ventricle dilatation, which consists in the fact that the patient's serum taken in the first three days after myocardial infarction, determine the level of tissue inhibitor of matrix metalloproteinase-1 type (TIMP-1) and the concentration of molecules intercellular adhesion to the vascular endothelium (sVCAM-1), calculate the coefficient of reparation (KR) according to the formula KP=TIMP-1/sVCAM-1 and when the value of CR more than 0,94 predict the development of post-infarction left ventricle dilatation.
SUBSTANCE: invention describes a method for measuring the immunomodulatory properties of cosmetic products, which involves the change determination of saliva dysenteric microbe antibody titer in saliva passive hemagglutination test (PHT); and the change determination of the antibody titer is specified in the saliva samples before the face, hands or body treatment with a cosmetic product and after that; the individuals being tested are divided into two groups; the first group of individuals with low antibody titer 1:128 or less are determined with the increased saliva antibodies after the face and body treatment with the cosmetic product, i.e. the immunomodulatory properties, and vice versa, the second group of individuals having antibody titers 1:256 or higher shows either no reaction, or the normalised content of the saliva humoral immunity factors.
EFFECT: method is simple, accurate, cost-saving and safe.
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FIELD: medicine, pharmaceutics.
SUBSTANCE: presented invention refers to medical microbiology, particularly to molecular-genetic typing of the strains Helicobacter pylori. What is presented is a method for differentiation of the strains H.pylori by multilocal VNTR-typing with the PCR involving oligonucleotide primers on VNTR-comprising loci of H.pylori - HpA, HpD, HpE and HpF; the strains H.pylori are differentiated by a number of repetitions in the amplified fragments in each VNTR-comprising locus of H.pylori - HpA, HpD, HpE and HpF that enables genetic typing of the studied strains.
EFFECT: what is presented is the method for differentiation of the strains Hpylori by multilocal VNTR-typing.
1 tbl, 3 ex
SUBSTANCE: what is involved is the immunogenetic analysis of venous blood of the patients with chronic myeloid leukemia treated with a first-generation tyrosine kinase inhibitor, Gleevec. Polymerase chain reaction PCR-SSP is used to identify the genes of the class II HLA system, locus B. If observing any specificities HLA-DRB1*15(02) or HLA-DRB1*16(02), the favourable outcome of chronic myeloid leukemia with an optimal response developing over the control period (6, 12 18 months of therapy) is predicted. In observing the specificities DRB1*11(05) or DRB1* 14(06), the unfavourable outcome of chronic myeloid leukemia with a failure to achieve an optimal response is predicted.
EFFECT: higher accuracy of prediction of the outcome of chronic myeloid leukemia with underlying Gleevec therapy.
9 tbl, 4 ex
FIELD: medicine, pharmaceutics.
SUBSTANCE: present invention refers to immunology. What is presented is a monoclonal antibody or a fragment thereof, which causes NK-cell induced lysis of human target cells carrying HLA-E on their surface, optionally conjugated with a detectable marker. The antibody or the fragment thereof are specific to NKG2A, have heavy and light chains, each of which contains 3 CDR and does not bind specifically: human NKG2C or NXG2E and Fc receptor. There are described: a pharmaceutical composition, a method for recovering the NK-mediated lysis of target cells, a conjugate, a set and a method for detection based on the use of the antibodies. What is disclosed is a composition for treating autoimmune and inflammatory diseases.
EFFECT: use of the invention can find application to stimulate the NK-cells leading to the lysis of dendritic cells, which contribute to the pathology of autoimmune and inflammatory diseases, that can find application in medicine.
36 cl, 4 dwg, 5 tbl, 12 ex
SUBSTANCE: for the purpose of the prediction of the delayed bone consolidation accompanying extrafocal osteosynthesis, preoperative blood CD3+ lymphocytes and CD 19+ lymphocytes are counted, and then the CD3+ lymphocytes/CD 19+ lymphocytes index is calculated. If the relation is more than 6.6 units, the postoperative delayed bone consolidation is predicted, and the value being 6.6 units and less enables predicting the normal clinical course of osteogenesis.
EFFECT: use of this method allows for the preoperative prediction of the bone consolidation pattern.
SUBSTANCE: for the purpose of early prediction of bronchopulmonary dysplasia, the 3-7-day newborn's blood serum is examined for interleukine-6 and interleukine-1 receptor antagonist. If interleukine-6 appears to increase more than 24 pg/ml, while the interleukine-1 receptor antagonist is more than 560 pg/ml, developing bronchopulmonary dysplasia is predicted.
EFFECT: early prediction of bronchopulmonary dysplasia, and respectively, the adequate therapeutic correction in the newborns with very low or extremely low body weight.
1 tbl, 6 ex
SUBSTANCE: whole blood samples are cultured in the presence of a Mycobacterium antigen. Then, the blood cells are treated with a mixture of monoclonal antibodies specific to CD4 and CD27 markers. The prepared mixture is incubated. Further, the cells are treated with monoclonal antibodies specific to IFN-γ, and then analysed on a flow cytofluorimeter. The percentage of CD27- lymphocytes among CD4+ lymphocytes producing IFN-γ is determined. The analysis is conducted in the beginning of and during the treatment. The decrease of the initial high value thereof more than 31.2% during the treatment to the normal values less than 31.2% is considered to be a sign of cavity closure or reduction in the lung tissue and the effective treatment of tuberculosis. The decrease of the initial high value by 40% or more without reaching the normal values and maintaining it above 31.2%, a positive course of tuberculosis and reduction of destructive processes in the lungs are predicted. No change of the value or the increase thereof during the treatment indicates the absence of the course of pathological changes and persistent manifestations of destructive changes in the lungs.
EFFECT: method enables the objective estimation of the clinical effectiveness in the tuberculosis infection without the use of additional analyses.
SUBSTANCE: invention describes a method for assessing the nerve tissue regeneration in the patients with complicated cervical spine injuries where a surgical intervention is preceded by blood sampling, preparing blood serum samples, examining blood serum by enzyme immunoassay, and the quantitative values of the levels of neurotrophin-3 NT-3 and neurotrophin-4/5 NT-4/5 are determined; a nerve tissue regeneration index is calculated by formula; the nerve tissue regeneration index is accepted to be a reference index Ireg.ref.; similarly, on every 7th postoperative day, current regeneration indices Ireg.cur. are calculated and compared to the reference; if Ireg.ref.>I reg.cur., a delayed process of the nerve tissue regeneration is stated; Ireg.ref.≤Ireg.cur., an activated process of the nerve tissue regeneration is stated.
EFFECT: invention enables higher objectivity of the assessment of the nerve tissue regeneration in the patients with complicated cervical spine injury with higher sensitivity and specificity of the declared method.
SUBSTANCE: invention describes a method for prediction of a risk of developing benign mammary dysplasia in females with genital endometriosis involving DNA recovery from peripheral venous blood, determination of gene polymorphism of type 1 tumor necrosis factor a receptor (+36 A/G TNFR1). Detecting the genotypes +36 AA TNFR1 and +36 AG TNFR1 enables predicting the high risk of developing benign mammary dysplasia in genital endometriosis.
EFFECT: use of the method enables predicting the risk of the onset of benign mammary hyperplasia in the patients with genital endometriosis and thereby determining the further approach to the patients with genital endometriosis.
1 tbl, 2 dwg, 1 ex
SUBSTANCE: method for prediction of essential hypertension in females with hypertensive disorders accompanying pregnancy with a family history of cardiovascular disease at a young age and episodes of high blood pressure at random measurements in the nonpregnant state on the basis of genetic testing. Buccal epithelium is sampled to recover DNA samples subject to polymerase chain reaction. The analysis results are used to specify the information signs and to calculate a prognostic index D by formula: D=1.68×OH+0.71×ITGB3+0.60×AGTR2-0.61×ITGA2+0.56×"П"+1.53×"АГ"-2.25. If D is less than 0, no risk of developing essential arterial hypertension is stated, and if D is more than 0, a high risk of developing essential arterial hypertension is predicted.
EFFECT: higher accuracy of the prediction procedure.
FIELD: medicine, ophthalmology.
SUBSTANCE: in lacrimal liquid one should detect the content of interleukin 8 (IL-8) and that of interleukin 1 beta (IL-1β) to calculate prognostic coefficient (PC) due to dividing the first value by the second one by the following formula: At PC value being below 10.0 one should predict favorable disease flow, and at PC value being above 10.0 - unfavorable flow.
EFFECT: higher accuracy of prediction.
FIELD: medicine, medicinal microbiology.
SUBSTANCE: method involves growing microorganism culture to be studied in solid nutrient medium followed by preparing microbial suspension and its incubation in the presence of lactoferrin. Control sample is prepared in parallel series. Control and experimental samples are incubated, supernatant is removed from bacterial cells and lactoferrin concentration is determined in supernatant of experimental and control sample by immunoenzyme analysis. Then anti-lactoferrin activity is calculated by difference of concentrations of residual lactoferrin in experimental and control samples. This method provides enhancing the sensitivity and precision in carrying out the quantitative evaluation of anti-lactoferrin activity in broad spectrum of microorganisms that is urgent in diagnosis and prognosis of diseases with bacterial etiology. Invention can be used in determination of persistent indices of microorganisms for assay of their etiological significance in pathological processes.
EFFECT: improved assay method.
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FIELD: medicine, biology.
SUBSTANCE: invention relates to nutrient medium used for accumulation of cells for the following cytological and/or immunocytochemical analysis carrying out. Invention relates to medium containing salts NaCl, KCl, anhydrous CaCl2, MgSO4 x 6 H2O, MgCl2 x 6 H2O, Na2HPO4 x 2 H2O, KHPO4, NaHCO3, and also glucose and Henx's solution, 10% albumin solution and polyglucin taken in the ratio 1:1:1. Invention provides enhancing the preservation of cells.
EFFECT: improved an valuable properties of nutrient medium.
FIELD: medicine, cardiology.
SUBSTANCE: in peripheral blood one should detect the level of CD95(+) and CD16(+) neutrophilic granulocytes and at combination of increased level of CD95(+) neutrophilic granulocytes by 4 times and more and CD16(+) neutrophilic granulocytes by 0.6 times against the norm with ECG signs of myocardial infarction one should predict lethal result of large-focal myocardial infarction.
EFFECT: higher accuracy of prediction.
FIELD: medicine, parasitology.
SUBSTANCE: one should carry out immunoenzymatic assay to detect diagnostic optic density and that of labeled immune complex in a plot's hole with tested serum measured in conventional units at wave length being 492 nm. One should calculate coefficient of antibodies concentration measured in conventional units by the following formula: CAC = (Odtsh - Odd) x 100, where CAC - coefficient of antibodies concentration, Odtsh - optic density of the hole with tested serum, Odd - diagnostic value of optic density, 100 - coefficient of serumal dilution. By CAC value one should detect the titer of antibodies to Lamblia intestinalis antigens to interpret results of the trial. The method enables to study the dynamics of disease flow.
EFFECT: higher efficiency and accuracy of diagnostics.
1 ex, 1 tbl
SUBSTANCE: the present innovation deals with studying and treating diseases of inflammatory, autoimmune and degenerative genesis. One should perform sampling of heparinized blood followed by its sedimentation to obtain blood plasma with leukocytes and centrifuging to isolate the latter which are washed against erythrocytic and serumal admixtures, and, also, it deals with calculating the number of cells in samples out of leukocytic suspension after incubation (B) for 1.5 h at 37 C in holes of plastic microplotting board, out of leukocytic suspension one should additionally prepare two samples, one should be applied to calculate total number of leukocytes before incubation (A), the second sample undergoes incubation at the same mode at addition of autoserum to calculate the number of cells remained after incubation (C). One should state upon adhesive properties of leukocytes by the index of spontaneous adhesion (D), where D=(A-B)/B.100%, and effect for enhanced cellular adhesion under the impact of autoserum should be detected by the value of K=(B-C)/C.100% at K ≥ 30%, where B - C - the number of cells undergone additional adhesion after addition of autoserum. The present innovation widens functional possibilities of the suggested method due to obtaining additional values depicting adhesive properties of blood leukocytes.
EFFECT: higher accuracy of detection.
FIELD: medicine, immunology.
SUBSTANCE: one should carry out reaction of blast-transformation, detect proliferation of T-lymphocytes activated with antibodies to CD3 in the presence of interleukin-7 (ACT IL-7) and in the presence of interleukin-7 and dexametazone (ACT IL-7 D), calculate the index for dexametazone action as the ratio of ACT IL-7 to ACT IL-7 D, moreover, the value of dexametazone action index being above 1.2 indicates increased production of cytokins that suppress T-lymphocytes in neonatals. The method enables to detect functional defect of immune system that characterizes neonatal period.
EFFECT: higher efficiency of detection.
SUBSTANCE: method involves measuring forced exhalation volume per 1 s (FEV1) in l, full right ventricle evacuation time (RVE) in ms and angiotensin II value (AII) in ng/l. Discriminant relationship is built as D=0.504·RVE+3.038·FEV1 - 2.0·AII. D being less than 83.88, pulmonary hypertension occurrence is predicted within 1 year. D being equal to or greater than 83.88, no pulmonary hypertension is predicted to occur.
EFFECT: enhanced accuracy of prediction.
FIELD: medicine, medicinal immunology.
SUBSTANCE: method involves determination of heterophilic antibodies in human serum blood by the Paul-Bunnel's method relatively the level of circulating immune complexes, complement-activating properties of heterophilic antibodies by incubation of standardized ram erythrocytes with 0.8% serum for 30 ± 5 min and the following measurement of the erythrocytes lysis degree. The measurement of the effector function coefficient of heterophilic antibodies is carried out by the complement system Keff.f.h.a.-c.s. by the formula: Keff.f.h.a.-c.s. = Y/Tg.a. wherein Y means a lysis degree, %; Tg.a. means a reverse titer of heterophilic antibodies to ram erythrocytes. The damage assay is carried out by comparison of the immune status with the relative level of circulating immune complexes in serum. Method provides detection of preclinic from of immunodeficiency and autoimmune diseases that opens the possibility for their prophylaxis at most early stages of development. Invention can be used for assay of damage in the immune status in human serum blood.
EFFECT: improved method for assay.
5 tbl, 1 ex
SUBSTANCE: method involves concurrently examining anti-inflammatory IL-4 level in blood serum and lacrimal fluid. The value being within the limits of 60-70 pg/l in blood serum and 5-15 pg/l in lacrimal fluid, disease prognosis is considered to be unfavorable. The IL-4 concentration being within the limits of 90-100 pg/l in blood serum and 20-30 pg/l in lacrimal fluid, disease prognosis is considered to be favorable.
EFFECT: high accuracy of diagnosis.