Method to monitor microbiological activity in technological flows

FIELD: biotechnologies.

SUBSTANCE: device comprises a through cell equipped with holes, where at least one hole represents an inlet hole for intake of fluid medium from the specified technological flow, and at least one hole is an outlet hole for discharge of fluid medium from the specified through cell. To one of specified holes an RK probe is attached, possibly, an OVP probe, a cleaning accessory. The first pipeline is connected to the inlet hole. Possibly, the second pipeline is connected to the outlet hole. A valve is connected to the specified through cell. With the help of the specified devices and methods they measure volume microbiological activity and surface microbiological activity in a process flow of water by means of measurement of dissolved oxygen concentration.

EFFECT: method improvement.

45 cl, 10 dwg, 2 tbl, 4 ex

 

The technical FIELD

The present invention relates to a device for monitoring microbiological activity in process streams and method of monitoring microbiological activity in process streams.

The LEVEL of TECHNOLOGY

Microbial growth in industrial water systems can lead to contamination and fouling of the equipment surfaces. If growth is not enough control, pollution can cause unpleasant odors and decrease in the functional activity of the additives (for example, microorganisms can produce catalase, because they use hydrogen peroxide to improve whiteness, and can produce cellulase, which can affect the strength of the fiber). In low level control fouling surface formed biofilms will affect the heat transfer and, in the case of systems used in the paper industry, the occurrence of biofilms can cause the need to slow down the production process and shutdowns for cleaning surfaces from sediments or dirt falling on the product surfaces can cause holes or stains on the finished paper or plates. Therefore, such water is treated with biocides to control microbial growth and prevent related problems.

Because akrasanee and biofilm leads to various problems in industrial water systems, as well as planktonic and sessile bacteria react differently to measures of biological control, there is a need to track the impact of biological control on different methods of microbial growth.

Standard methods that are commonly used for monitoring of aquatic systems include standard Cup of count defining the number of microorganisms by culture on a Petri dish). These methods require long incubation periods and do not provide reliable information necessary for proactive (preventive) control and prevention of problems associated with microbial growth. In the recent past as a means of preventive control was applied analysis using adenosine triphosphate (ATP). However, the used reagents are expensive, and, in addition, large water systems selected small volume water samples. Data collection also produce infrequently, resulting in significant gaps in the data. Thus, this approach provides only limited information about the state of the microorganisms in the system. In addition, this approach is usually used for monitoring of planktonic bacteria. Although in some cases, removal of the surface layer and its analysis with the purpose of counting the number of the tion of bacteria, in biological film. These methods are very time consuming and require considerable time.

For measurement of microbial activity in fluids using probes to determine the amount of dissolved oxygen (do), because it is well known that microbial activity and aerobic metabolism lead to the reduction of dissolved oxygen concentration. In U.S. patent No. 5190728 and 5282537 described method and equipment for monitoring fouling in industrial water, using measurements of RK. However, this method requires the use of nutritional supplements to distinguish biological fouling from biological fouling, and patents there is no mention of how the probe is recovered for further measurements after fouling surface of the probe. In addition, this approach requires a means of continuous supply of oxygen.

Standard electrochemical probe Clark (Clark) to determine the ROK has many limitations, for example: chemical interference (H2S, pH, CO2, NH3, SO4, Cl-, Cl2, ClO2, MeOH, EtOH and various ion particles), frequent calibration and replacement of the membrane, slow response and unstable readings, the effect of thermal shock and the need for a high flow rate through the membrane. A new type of probe for determining alvarenga oxygen (probe RK), which recently delivers a number of manufacturers (e.g., HACH, Loveland, CO), practically does not have these limitations, and thus, RK can be measured in the technological waters online. The work of this new probe to determine RK (LRC) is based on measuring the duration of fluorescence decay, when the presence of oxygen reduces the duration of the fluorescence of the excited fluorophore. The fluorophore immobilized in the film on the surface of the sensor and provide excitation with blue light emitting diode (LED).

In U.S. patent No. 5698412 and 5856119 described method of monitoring and regulation of biological activity in fluids, in which RK is measured with a pH that allows you to track changes in metabolic behavior, in particular associated with the depletion of nutrients/substrate.

There remains a need to develop reliable and convenient ways of monitoring planktonic bacteria and bacterial biofilms contained in industrial waters, which can ensure adequate implementation of biological control of pollution and undesirable biological films. In these methods should not be used reagents that will allow the measurement of microbial activity in conditions typical of the environment (the minimal changes). These methods should be automated, and to enable remote control from the monitor, remote access, and remote or automated feedback control for the implementation of biological control. Theoretically, these methods should provide a definition of the differences between microbial activity on the surface and the total activity in the water, which will determine an appropriate response of biological control on the problems that usually arise when trying to control microorganisms in biological films. In addition, these methods should provide information about the nature of the sediments (biological or non-biological), which in turn promotes the application of appropriate control measures.

The INVENTION

The present invention provides a device for measuring microbiological activity in a process stream, comprising: (a) a flow cell provided with holes, where at least one opening is an inlet flow cells for sampling fluid from the specified process stream and at least one opening is an outlet for release of fluid from the specified flow cell; (b) probe RK attached to one of these from the of Erste; (c) perhaps the ORP probe attached to one of these holes; (d) a cleaning device attached to one of these holes; (e) perhaps the first pipeline connected to the inlet hole of the flow cells; (f) possible, a second pipe connected to the outlet flow cells, and (g), possibly a valve attached to the specified flow cell.

The present invention also provides a method of monitoring the bulk (total) microbiological activity of water in the process stream, comprising: (a) attaching the device to the process flow stream, with the specified device includes a flow cell provided with holes, where at least one opening is an inlet flow cells for sampling fluid from the specified process stream and at least one opening is an outlet for release of fluid from the specified flow cells; probe RK attached to one of these holes; perhaps the ORP probe attached to one of these holes; may, cleaning device attached to one of these holes; perhaps the first pipeline connected to the inlet hole of the flow cells; it is possible that the second pipeline, to recognize the military to the outlet flow cells, and, perhaps, a valve attached to the said flow cell; (b) sampling the fluid from the specified process flow specified in the flow cell; (c) opening the valve of the specified device for the transmission of fluid at a specified flow cell; (d) at least one measurement of the concentration of the Republic of Kazakhstan in the specified process stream using the specified probe RK, and prior to each measurement the surface of the specified probe RK clean; (e) closing of the valve device for terminating the selection of the fluid at a specified flow cell; (f) at least one concentration measurement RK fluid the environment inside the unit, using the specified probe RK, and prior to each measurement the surface of the specified probe RK is cleaned; (g) calculating the difference Δ between the readings obtained in stage (d) and stage (f), and (h) establishing a correlation at least between the specified value Δ obtained in stage (g), and bulk (total) microbiological activity in the specified process stream.

The present invention also provides a method of measuring the surface microbiological activity in a process stream, which comprises: (a) attaching the device to the process flow stream; and said device includes a flow cell, where is nnow holes, where at least one opening is an inlet flow cells for sampling fluid from the specified process stream and at least one opening is an outlet for release of fluid from the specified flow cells; probe RK attached to one of these holes; perhaps the ORP probe attached to one of these holes; perhaps a cleaning device attached to one of these holes; perhaps the first pipeline connected to the inlet hole of the flow cells; possibly, a second pipe connected to the outlet flow cells, and possibly the valve attached to the said flow cell; (b) sampling the fluid from the specified process flow specified in the flow cell; (c) opening the valve of the specified device for the transmission of fluid at a specified flow cell; (d) at least one measurement of the concentration of the Republic of Kazakhstan in the specified process stream using the specified probe RK, and prior to each measurement, the specified probe RK is not clear; (e) cleaning the surface of the specified probe RK; (f) at least one concentration measurement RK fluid inside the device, using the specified probe RK, and at the same time, who is one, before each measurement the surface of the specified probe RK is cleaned; (g) calculating the difference Δ between the readings obtained in stage (d) and stage (f), and (h) establishing a correlation at least between the specified value Δ obtained in stage (g), and surface microbiological activity.

The present invention also relates to a method of monitoring as in the bulk (total) microbiological activity and surface microbial activity.

BRIEF DESCRIPTION of DRAWINGS

Figure 1 schematically shows a device comprising a flow cell, the probe RK, a cleaning device and, possibly, a probe for measuring the redox potential.

Figure 2 schematically shows the device installed on the mounting plate inside the housing; the device includes a flow cell, the probe RK, probe to determine the redox potential, the cleaning device comprising a solenoid, wiper, the first pipeline, the second pipeline and valve.

Figure 3 schematically shows a device which includes a probe RK, probe to determine the redox potential and purifying device.

Figure 4 schematically shows a device that includes a flow cell, the probe RK, probe to determine the redox potential and the cleaning device, comprising a scraper wiper.

Figure 5 schematically shows the flow cell and the element used to increase the surface area.

Figure 6 presents the data obtained at a paper mill that apply to bulk (total) microbiological activity and fouling of surfaces.

Figure 7 presents the data obtained at a paper mill, which are related to the bulk (total) microbiological activity and fouling of surfaces.

On Fig presents the block diagram of the monitoring bulk microbiological activity and/or surface microbial activity.

Figure 9 presents one example implementation of the present invention includes a flow cell attached to the probe RK, the probe for measuring the redox potential and purifying device.

Figure 10 presents one example implementation of the present invention, including ODO and a flow cell attached to the probe RK, the probe for measuring the redox potential and purifying device.

DETAILED description of the INVENTION

Definition of terms

"RK" means dissolved oxygen.

"Probe the Republic of Kazakhstan" includes probe of any type, which can be measured dissolved oxygen concentration. Preferably the probe RK is a probe for measuring dissolved oxygen luminescence method ("probe RK"). "LRC" means the concentration of dissolved oxygen, measured by the luminescence method. Zones is s LRC (probes for measuring the concentration of dissolved oxygen luminescence method) allows to determine the dissolved oxygen concentration on the basis of the duration of fluorescence decay, where the presence of oxygen reduces the duration of the fluorescence of the excited fluorophore. The fluorophore immobilized in the film on the surface of the sensor and provide excitation with blue LED. Probes LRC supplies Hach Company, Loveland, CO. Probes typically have a sensor head, which record the measurement.

"ORP" means the redox potential. Probes for determination of AFP delivers Walchem Corporation, Holliston, MA.

"REDOX" means the state of oxidation-reduction.

"ALC" means optical sensor fouling. For this purpose, can be used in any optical sensor fouling, suitable for a particular method. It includes any conventional measuring tool to determine sediment, such as quartz microbalance.

"Valve" means any tool that allows you to adjust the flow of fluid.

"Cleaning device" is any device(I), which produce a clean surface, such as surface probe of the Republic of Kazakhstan and/or the surface of the ORP probe.

"Process stream" means any of the fluid used in the implementation of industrial way, for example, the fluid selected from the pipeline installation manufacture of paper, and the fluid selected from polyparasitism the La setup the manufacture of paper.

PREFERRED embodiments of the PRESENT INVENTION

Microbial activity in process streams can be measured by indirect methods, by monitoring the consumption of dissolved oxygen, because the consumption of dissolved oxygen is directly related to the amount of ATP produced by the cells during aerobic respiration, and the amount of ATP produced by the cells depends on the level of microbial activity in these process streams. The methods described in the present invention, unsuitable for measurements in process streams with low levels of RK, in which aerobic respiration is not the primary method of energy production in the cells of microbes.

Data on measurements of RK obtained in the process flows must be converted to a percent of the saturation concentration, obtained on the basis of indicators of pressure, temperature and salinity of flow. This helps to normalize the data, including technological fluctuations of these parameters. Particularly important correction temperature because during the analysis produced by stopping the flow at which to stop screening the fluid in the flow cell, the temperature of the process streams is reduced by 1-10 degrees Celsius.

To improve dostovernost the ratio between the consumption of dissolved oxygen and microbial activity of the REDOX state of the process fluid must be oxidizing, to oxygen consumption did not occur as a result of chemical oxidation processes. On the REDOX state of flow is affected by factors such as pH. High pH values, for example, if the pH of the process water exceed 9.5, can lead to oxidation of organic materials in the processing fluids even at high REDOX conditions.

Thus, preferably, the ORP process stream should be measured together with the concentration of the Republic of Kazakhstan to ensure that the consumption of dissolved oxygen is primarily associated with microbiological activity, and not with chemical reactions occurring in the process stream.

A. the DEVICE

The device was designed for practical measurement of dissolved oxygen in the process flows. The proposed device can be attached to other analytical tools, such as the ORP probe (probe for measuring ORP).

As shown in figure 1, the device includes a flow cell (1); probe (2) RK; perhaps the probe (3) redox potential; and a cleaning device (7).

Flow cell (1) is provided with holes. These holes serve to allow the flow of fluid through the flow cell (1). The size and shape of holes may vary; in particular, you should consider the type tehnologicheskogo the stream.

From Figure 3 it is seen that the flow cell (1) includes an inlet (13) and outlet (14). The diameter of the holes should be large enough for the free flow of fluid from the process stream through the flow cell (1) and prevent clogging of the flow cell (1), as well as prevent non-biological fouling as the surface of the probe (2) RK, and the surface of the probe (3) ORP. Thus, the diameter of the flow cell (1) depends on many factors, such as the type of the process stream.

The holes in the flow cell can also be used to join various means, such as a probe (2) RK, probe (3) ORP and/or purifying device (7) to perform one or more measurements of the parameters of the process stream. To the flow cell can be attached and other tools, such as measuring the acidity (pH-meter).

In particular, to a flow cell (1) attach the probe (2) the Republic of Kazakhstan and/or the probe (3) AFP.

In one of the embodiments of the present invention, the probe (2) RK and the probe (3) ORP attached to the flow cell. The probes can be attached to one of the openings of the flow cell (1) in a variety of ways known to specialists in this field of technology. The joining may be effected by any means of connection and/or installation or podobn what x means. For example, in a flow cell (1) may be mounted into a device, and this device may be a probe/tool, which is rigidly secured in place.

As shown in Figure 3, the probes are installed flush with the wall of the flow cell (1).

In one of the embodiments of the present invention at least a part of the said probe (2) RK and possibly probe (3) ORP goes inside the specified flow cells.

In another embodiment of the present invention, the probe (2) RK includes touch the head of the RK, and at least a portion of a specified sensor head RK takes place inside the specified flow cells, and, possibly, with the indicated probe (3) ORP includes a sensor head to determine the redox potential, and at least a portion of a specified sensor head to determine the ORP goes inside the specified flow cells.

In another embodiment of the present invention, the probe should be oriented in such a way as not to prevent substantially the flow of the fluid flowing through the flow cell (1).

In another embodiment of the present invention, the probe (2) RK and the probe (3) ORP are located opposite each other.

Figure 2 presents additional characteristics of the device. More specifically, figure 2 presents the first pipeline is ar (4), valve (6)connected to the first pipe (4), Stoke (15)attached to the first pipe (4), flow cell (1), probe (2) RK, probe (3) ORP, the cleaning device (7), the solenoid (9)attached to the specified cleaning device (7), and a second pipe (5).

The first pipe (4) and a second pipe (5) connected to one or more holes at the specified flow cell (1), as well as to the case in which the process stream. Joining may be accomplished in various ways known to the skilled in the art. For example, the first pipe (4) can be attached to the process flow stream through a system of pipes.

The first pipe (4) is used for input and/or removal of fluid from the process stream in a flow cell (1) and/or other means, for example, ALC. The first pipe (4) can be positioned in any way facilitating the movement of fluid from the process stream in a flow cell (1). For example, a mechanism driven by gravity or by applying energy, for example, the pump may take the fluid from the process stream in a device that includes a flow cell (1).

In another embodiment of the present invention to prevent reverse current and/or limit the flow of those is technological flow, to the first pipe (4) can be attached flow (15).

The second pipe (5) is used to release the fluid flowing through the flow cell (1), and also as a reservoir for storing the fluid from the process stream. In particular, the second pipe (5) can be oriented in space so that fluid is retained inside the flow cell (1) for measurements in terms of stopping the flow. For example, the second pipe (5) is positioned so that fluid can be maintained within the flow cell (1) under the action of gravity.

In another embodiment of the present invention, the second pipe (5) can also act as a drain.

Valve (6) is attached to the flow cell (1). In particular, the valve (6) is attached to the flow cell (1) in such a way as to ensure its desired destination. The valve(s) control(s/regulate(s) the flow of fluid from the process stream in a flow cell (1).

In one of the embodiments of the present invention, the valve (6) is attached to the flow cell through the first pipe (4). In particular, the valve (6) is inserted/attached to the first pipe (4) so that in the closed position of the valve the flow is limited, and in the open position of the valve (6) flow occurs freely.

In another note the re implementation of the present invention with the valve(s) (6) it is possible to regulate the flow of fluid in ALC and/or in a flow cell(1).

In another embodiment of the present invention the diameter of the valve (6) must be large enough not to impede the flow of process water with high solids content.

In another embodiment of the present invention, the valve (6) can also prevent the leakage of fluid from the flow cell (1) or a second pipeline (5), which allows you to register indication of stopping the flow.

In another embodiment of the present invention the diameter of the valve (6) is at least 2.54 cm (one inch).

In another embodiment of the present invention, the valve (6) is a ball valve.

In another embodiment of the present invention, the valve (6) actuate manual, electric mechanism or a pneumatic mechanism.

In another embodiment of the present invention ball valve (6) actuate manual, electric mechanism or a pneumatic mechanism.

Figure 2 and 4 show that the cleaning device (7) can be attached to one of the holes flow cells (1). The cleaning device is used to clean the surface of the probe (2) the Republic of Kazakhstan and/or the surface of the probe (3) ORP, and the location of the specified device must ensure that pointed to by the second function. The cleaning device (7) can cleanse other means attached to the flow cell (1).

In one of the embodiments of the present invention provide the ability to move the cleaning device (7) on the surface of the flow cell (1).

In another embodiment of the present invention provide the ability to move the cleaning device (7) on the surface of the flow cell (1) and purification of one or more tools/probes, such as probe (2) RK, probe (3) ORP or other types of analytical tools that can be attached to the flow cell (1).

In another embodiment of the present invention the cleaning device (7) includes a wiper (8) or brush.

In another embodiment of the present invention the cleaning device (7) is brought into action by means of a solenoid (9) of the wiper. The solenoid (9) receives commands from a controller, which is provided with a logical circuit, the serving team when you need to clean and there is no need to clean up.

As shown in figure 4, the wiper (8) is mounted for movement on flow cell in the direction perpendicular to both the probe (2) RK, and the probe (3) AFP.

Add one or more partitions (11) in a flow cell (1) can increase the area of flowing the cells (1). Figure 5 presents a modified flow cell. Specifically, to a flow cell attached element includes more than one partition. The element can be attached to the flow cell in several ways. Similarly, you can also use other tools that can increase the surface area.

In one of the embodiments of the present invention, the element (10) is fixed to the flow cell (1) by means of an adapter (12). The item includes the inlet opening (15) of the element, which flows from the specified process flow, and the exhaust port, which is attached to the flow cell.

In one of the embodiments of the present invention, the first pipe (4) is first attached to the item (10) instead of attaching it directly to the flow cell (1).

In another embodiment of the present invention, the element (10) includes one or more baffles (11).

The device may be adapted to monitor the bulk (total) microbiological water activity, surface microbiological activity, or combinations thereof.

B. MONITORING the BULK (TOTAL) MICROBIOLOGICAL ACTIVITY IN a PROCESS STREAM

The present invention relates to a method of monitoring the bulk (total) microbiological activity of the process stream. Bulk (total) microbiological activity means microbiological activity in the volume of the process stream, such as planktonic microorganisms and sessile microorganisms in the process stream.

Bulk (total) microbiological activity process flow is determined by measuring the concentration of the Republic of Kazakhstan in the process stream. In conjunction with this parameter can be measured, and other parameters. More specifically, the method includes the following stages: (a) attaching the device to the process flow stream, with the specified device includes a flow cell provided with holes, where at least one opening is an inlet flow cells for sampling fluid from the specified process stream and at least one opening is an outlet flow cells for release of fluid from the specified flow cells; probe RK attached to one of these holes; perhaps the ORP probe attached to one of these holes; perhaps a cleaning device attached to one of the specified holes; perhaps the first pipeline connected to the inlet hole of the flow cells; possibly, a second pipe connected to the outlet flow cells, and, who is one, the valve attached to the said flow cell; (b) sampling the fluid from the specified process flow specified in the flow cell; (c) opening the valve of the specified device for the transmission of fluid at a specified flow cell; (d) at least one measurement of the concentration of the Republic of Kazakhstan in the specified process stream using the specified probe RK, and prior to each measurement the surface of the probe RK clean; (e) closing the valve of the specified device to prevent selection of the fluid at a specified flow cell; (f) at least one concentration measurement RK fluid inside the specified device using probe RK, and prior to each measurement the surface of the probe RK is cleaned; (g) calculating the difference Δ between the readings obtained in stage (d) and stage (f), and (h) establishing a correlation at least between the specified value Δ obtained in stage (g), and bulk (total) microbiological activity in the specified process stream.

This method can be used for different types of flow.

In one of the embodiments of the present invention the process stream is a process stream from a process selected from the group consisting of a papermaking process, process chilled the water, processing of food or beverage and process of recreational water use.

Bulk microbiological activity in the water is measured by monitoring changes in the concentration of the Republic of Kazakhstan (Δ) during the flow and stop flow. Together with the specified parameter can be measured, and other parameters. More specifically, the evaluation value Δ allows to determine the rate of consumption of the RK. Then there may be a correlation between the rate of consumption of RK and microbiological activity of the specified process flow, and the reliability determination will be higher, if together with RK measure ORP, because if the REDOX state of the fluid process stream is not oxidative, this may affect the measured value of RK.

Under the terms of the leakage flux understand the conditions when the fluid process stream may pass through a flow cell in which produce measurements using analytical equipment, which is attached to the flow cell, in particular, by using the probe RK, designed for measuring the concentration of the Republic of Kazakhstan fluid medium.

Under the terms of stopping the flow understand the conditions when the fluid process stream can no longer flow into the flow cell. In terms of stopping the flow of the fluid retention is more in the flow cell, where is tracking the concentration of the Republic of Kazakhstan specified fluid.

In terms of the flow stream, for example when carrying out stage (d), the concentration of the Republic of Kazakhstan fluid process stream is measured in a period of time sufficient to obtain an accurate reading of the concentration of the Republic of Kazakhstan in the process stream. For this purpose it is necessary to make one dimension or more. Specialist in the art will be able without undue experimentation to determine the number of measurements needed to obtain accurate readings of the flow of technology and will be able to determine the time intervals between measurements that are necessary to get an accurate reading process stream.

In terms of stopping the flow, both at the stage (f), before the first measurement of RK fluid in the flow cell must undergo a significant amount of time to ensure that one or more of microbiological organisms in the environment had enough time to consume the oxygen dissolved in the specified fluid. This time period may be different and depends on one or more factors, which may include the type of process that track, and the microbiological effectiveness of the program, which is used before the application of the method according to the present invention. For example, isopropylene water, used in the manufacture of paper, heavily contaminated with microorganisms, for consumption RK microorganisms may require less time. Types of microorganisms (e.g., filamentous fungi, or bacteria) can also affect the speed and degree of consumption of the Republic of Kazakhstan.

In one of the embodiments of the present invention, the measurement in terms of flow and stop flow of produce through the same intervals. In another embodiment of the present invention, the measurement in terms of flow and stop flow produced within the same time period and same intervals of time.

The monitoring process stream can be performed continuously, periodically or only once. Continuous monitoring provides real-time, allowing you to quickly identify system failures in the process stream.

Δ can be calculated in various ways.

In one of the embodiments of the present invention bulk microbiological activity is measured by calculating the maximum concentration change of Kazakhstan during the period of continuous water flow (in terms of leakage flow) relative to the concentration in terms of stopping the flow in which flow of water is stopped by closing the valve. The other is the capture, to calculate LRC use the maximum change in concentration of the Republic of Kazakhstan on the basis of evidence obtained in stage (d) and stage (f).

In another embodiment of the present invention is Δ determine, choosing the average measurement result of the RK on stage (d) and the minimum measurement result of the RK on the stage (f).

In another embodiment of the present invention is Δ determined by choosing the maximum measurement result at stage (d) and the minimum measurement result of the RK on the stage (f).

In another embodiment of the present invention is Δ determine, choosing the last measurement result stage (d) and the minimum measurement result of the RK on the stage (f).

In another embodiment of the present invention the duration of the measurement and the time interval between measurements at stage (d) and stage (f) is the same.

Even in one of the embodiments of the present invention the duration of the measurement phase (d) and stage (f) may be from 5 to 240 minutes.

Even in one of the embodiments of the present invention the duration of the measurement is 30 minutes and the measurement record 5 times on stage (d) and steps (f) through equal intervals of time.

Even in one of the embodiments of the present invention the surface is thoroughly cleaned for 30 seconds before recording is of perform on stage (d) and stage (f).

AFP process stream can be measured together with the measurement of the concentration of the Republic of Kazakhstan in the process stream.

In one of the embodiments of the present invention the method further includes at least a single measurement of redox potential on stage (d) and phase (f) and cleaning the surface of the ORP probe before each measurement.

In another embodiment of the present invention, if the value of the ORP falls below the specified level, then process flow you can add one or more oxidants.

In another embodiment of the present invention, if the measured(s) value(I) redox potential falls(ut) below a preset level, the values of RK, which are measured together with these values, ORP, do not take into account when calculating Δ. More specifically, the exclusion of these values allows the operator to better determine whether the level of consumption of RK to microbiological activity or chemical process stream.

In another embodiment of the present invention, if a given level of ORP is less than approximately 100 mV, the measured values of RK are not taken into account because if the ORP is in this range, the conditions are usually non-oxidizing and consumption of dissolved oxygen may refer to the chemical conditions of the process stream.

Response to the degree of volume is (full) microbiological activity in a process stream may be different.

In one of the embodiments of the present invention, if the degree of bulk (total) microbiological activity is high or above a specified level that is considered adequate for the process, the Protocol includes adding an effective amount of biocide to bring the degree of microbiological activity to the desired value.

Biocides may be oxidizing and/or non-oxidizing.

The biocides used in the papermaking process, selected from the group consisting of isothiazoline; glutaraldehyde; dibromonitrilopropionamide; carbamate; Quaternary ammonium compounds; sodium hypochlorite; chlorine dioxide; peracetic acid; ozone; chloramines; Stabrex™(promulgate); bromchloromethane; dichlorodimethylsilane; monochloramine; sodium hypochlorite, used in combination with ammonium salts and stabilizers, including dimethylhydantoin, amino acid, cyanuric acid; succinimide and urea; and combinations of the listed compounds.

To control the degree of microbiological activity process flow you can use one or more controllers. More specifically, the controllers can be programmed for obtaining the parameters of the process stream, such as indications of the probe RK, calculate the value Δ on the basis of the logic the scheme, entered in the controller (for example, program logic controller), and perform response actions in accordance with Δ, which may include various operations, such as the actuation of the pump, which delivers the biocide or polymers, which regulates the formation of deposits in the process stream.

In one of the embodiments of the present invention the controller is Web-based.

In another embodiment of the present invention, the controller may be in the message at least one of the following: probe ORP probe RK, purifying device, a valve, or with their combination.

In another embodiment of the present invention, the controller receives input signals from the specified probe RK, then it performs the required Protocol that is programmed in the specified controller.

In another embodiment of the present invention the controller is a control system. The term "management system" and similar terms refer to the operator manual control or electronic device that includes the following components: a processor, a storage device, a cathode ray tube, liquid crystal display, plasma display, touch screen or other display and/or other components. In some examples, the implementation of the of the controller can be combined with one or more specialized integrated circuits, programs or algorithms, one or more computing devices and/or one or more mechanical devices. Some or all functions of the device control system can be located centrally, for example in a network server that is designed to communicate over LAN, WAN, network, wireless Internet connection, microwave link, an infrared link, etc. in Addition, to facilitate the signal processing system can include other components, such as driver signals or system monitor.

In another embodiment of the present invention the desired Protocol includes alert the operator or person responsible for monitoring the process stream and processing the process stream.

In another embodiment of the present invention the desired Protocol program includes adding an effective amount of biocide in the process stream if the specified value is Δ reaches a predetermined level. The biocide may be oxidizing and/or non-oxidizing.

Optical sensor fouling (ODL) can be attached to the specified flow cell to determine the nature/origin of the sediments, which are formed in the process stream.

In one example implementation of the present and the acquiring method further includes: providing an optical sensor fouling, connected with the specified process stream; selection of fluid from the specified process stream in the specified optical sensor fouling; measurement and scaling of the optical sensor fouling; determining the type of sediments by correlation the formation of deposits in the specified optical sensor fouling with the specified microbiological activity, determined from Δ in the specified process flow; it is possible that the programming of the controller connected to the specified ODO and at least one probe RK, to add to the specified process flow of one or more chemical substances, depending on the correlation of the specified deposits microbiological activity.

In another embodiment of the present invention, if this correlation shows that the formation of deposits on the optical sensor fouling is microbiological in nature, the chemicals contain a biocide. For example, if the ODO is the deposition and Δ high, then one way is to add biocide to the specified process flow to combat the formation and reduction of microbiological activity in the process stream. Biocides may be oxidizing and/or non-oxidizing.

Another example is the implementation of the present invention if this correlation shows the formation of deposits has necrobiology nature, the chemicals are chemical substances that regulate the formation of deposits. For example, if the ODO is the deposition and Δ low, then one of the ways is adding to the process flow of the chemical substances that regulate the formation of deposits, to combat the formation of deposits. There are different types of chemicals to control sediments, well-known specialist in the art; for example, substances that prevent the resin formation, which help prevent Deposit formation in the manufacture of paper, as well as polymers that regulate the formation of deposits.

C. MONITORING of SURFACE MICROBIOLOGICAL ACTIVITY IN a PROCESS STREAM

Surface microbiological activity, referred to as the microbial activity of the surface of microorganisms, such as biofilms.

Surface microbiological activity process flow is determined by measuring the concentration of the Republic of Kazakhstan in the process stream. Along with this analysis, you can use other parameters. More specifically, the method includes the following operations: (a) attaching the device to the process flow stream, with the specified device includes a flow cell, sabien the Yu holes, where at least one opening is an inlet flow cells for sampling fluid from the specified process stream and at least one opening is an outlet flow cells for release of fluid from the specified flow cells, probe RK attached to one of these holes, perhaps the ORP probe attached to one of these holes, possibly cleaning device attached to one of these holes, perhaps the first pipeline connected to the inlet hole of the flow-through cell, possibly, a second pipe connected to the outlet flow cells, and, perhaps valve attached to the said flow cell; (b) sampling the fluid from the specified process flow specified in the flow cell; (c) opening the valve of the specified device for the transmission fluid specified in the process stream; (d) at least one measurement of the concentration of the Republic of Kazakhstan in the specified process stream using the specified probe RK, and the specified probe RK is not cleaned before each measurement; (e) cleaning the surface of the specified probe RK; (f) at least one concentration measurement RK fluid inside the specified device when polosukhina probe ROK and perhaps before each measurement the surface of the specified probe RK is cleaned; (g) calculating the difference LRC between the readings obtained in stage (d) and stage (f), and (h) establishing a correlation at least between the specified value Δ obtained in stage (g), and surface biological activity.

This method can be used for various types of flow.

In one of the embodiments of the present invention the process stream is a process stream from a process selected from the group consisting of a papermaking process, process cooling water, process food or beverage and process of recreational water use.

The activity of biofilms is calculated by the difference between the results of measurements of the Republic of Kazakhstan, taken before cleaning and after cleaning in terms of the flow stream. Together with the specified analysis it is possible to use other parameters. The reliability of determining the ratio between Δ and activity of biofilms above, if along with RK measure the ORP, as the result of a measurement of RK may change if the REDOX state of the fluid process stream is not oxidative.

Under the terms of the leakage flux understand the conditions when the fluid process stream can in order to flow through the flow cell and can be measured by the analytical instrument, being in communication with the flow cell, in particular a probe RK for measuring the concentration of the Republic of Kazakhstan fluid medium.

In terms of the flow stream, as in stage (d) and stage (f), must pass a sufficient amount of time before measuring the Republic of Kazakhstan, as this is the amount of time necessary for the accumulation of biofilm. This time period may be different and depends on various factors, including the type of process that track, and the microbiological effectiveness of the program used prior to the implementation of this method. For example, if the industrial water used in the production of paper, heavily contaminated with microorganisms, for consumption RK specified microorganisms may require less time. Types of microorganisms (e.g., filamentous fungi, or bacteria) can also affect the speed and degree of consumption of the Republic of Kazakhstan.

In one of the embodiments of the present invention, the measurement in terms of flow and stop flow of produce through the same intervals. In another embodiment of the present invention, the measurement in terms of flow and stop flow produced within the same time period and same intervals of time.

The monitoring process stream can be performed continuously, periodically or odnocratno.sochetanie monitoring provides real-time, that allows you to quickly identify system failures in the process stream.

Indicators Δ can be calculated in different ways.

In one of the embodiments of the present invention is Δ calculate, choosing the lowest measurement result of the RK on stage (d), and the average measurement result of the RK on the stage (f).

In another embodiment of the present invention is Δ calculate, choosing the lowest measurement result at stage (d) and the highest measurement result of the RK on the stage (f).

In another embodiment of the present invention is LRC calculate, choosing the last measurement result of the RK on stage (d) and the highest measurement result of the RK on the stage (f).

In another embodiment of the present invention dimension RK conduct and record 5 times during the selected period of time in the continuous flow mode, but before the specified dimensions cleaning the probe by means of the wiper is not produced.

In another embodiment of the present invention, the time delay before the selected time interval is one minute, after which produce clean probes and produce two consecutive measurements and recording the results.

AFP process stream can be measured together with the measurement of the concentration of the Republic of Kazakhstan in the process stream.

<> In one of the embodiments of the present invention the method further includes at least a single measurement of redox potential on stage (d) and phase (f) and cleaning the surface of the ORP probe before each measurement, stage (d) probe for measuring the redox potential is not fully clear and, perhaps, the specified probe for measuring ORP completely clear on the stage (f). Perhaps, if the value of the ORP falls below the specified level, process flow, you can add one or more oxidants.

In another embodiment of the present invention, if the results of measuring the ORP falls below the specified level, the results of measurement of RK obtained with these measurements AFP, may not be taken into account when calculating Δ, which are used to determine the microbiological activity in the process stream. More specifically, the exclusion of these results allows the operator to better determine whether the level of consumption of RK to microbiological activity or chemical process stream.

In another embodiment of the present invention, if a given level of ORP is less than approximately 100 mV, the values of RK are not taken into account because if the AFP level is in this range, the conditions are not oxidation and consumption of dissolved oxygen may be due khimicheskii conditions of the process stream.

In another embodiment of the present invention the probe RK, probe to determine the ORP or both of the probe should be cleaned with the cleaning device, which includes a wiper.

In another embodiment of the present invention the surface of the probe (probes) is wiped with a wiper twice.

Response to the degree of surface microbiological activity in a process stream may be different.

In one of the embodiments of the present invention, if the degree of surface microbial activity is high or above a specified level that is considered adequate for the process, the Protocol includes adding an effective amount of biocide to bring the degree of microbiological activity to the desired value.

Biocides may be oxidizing and non-oxidizing.

The biocides used in the methods of making paper, which are selected from the group consisting of isothiazoline; glutaraldehyde; dibromonitrilopropionamide; carbamate; Quaternary ammonium compounds; sodium hypochlorite; chlorine dioxide; peracetic acid; ozone; chloramines; Stabrex™ (promulgate); bromchloromethane; dichlorodimethylsilane; monochloramine; sodium hypochlorite, used in combination with ammonium salts and stabilizers, including di is elgiganten, amino acid, cyanuric acid, succinimide and urea, and combinations of the listed compounds.

To control the degree of microbiological activity process flow you can use one or more controllers. More specifically, the controllers can be programmed for obtaining the parameters of the process stream, such as indications of the probe RK, calculate the value Δ on the basis of logic, introduced in the controller (for example, program logic controller), and perform response actions in accordance with Δ, which may include various operations, such as the actuation of the pump, which delivers the biocide or polymers, which regulates the formation of deposits in the process stream.

In one of the embodiments of the present invention the controller is Web-based.

In another embodiment of the present invention, the controller may be in the message at least one of the following: probe ORP probe RK, purifying device, a valve, or with their combination.

In another embodiment of the present invention, the controller receives input signals from the specified probe RK, then it performs the required Protocol that is programmed in the specified controller.

In another embodiment, altoadige of the invention the controller is a control system. The term "management system" and similar terms refer to the operator manual control or electronic device that includes the following components: a processor, a storage device, a cathode ray tube, liquid crystal display, plasma display, touch screen or other display and/or other components. In some embodiments, the controller may be combined with one or more specialized integrated circuits, programs, or algorithms, one or more computing devices and/or one or more mechanical devices. Some or all functions of the device control system can be located centrally, for example in a network server that is designed to communicate over LAN, WAN, network, wireless Internet connection, microwave link, an infrared link, etc. in Addition, to facilitate the signal processing system can include other components, such as driver signals or system monitor.

In another embodiment of the present invention the desired Protocol includes alert the operator or person responsible for monitoring the process stream and processing the process stream.

In another embodiment, this image is placed to the desired Protocol program includes adding an effective amount of biocide to the process flow stream, if the specified Δ reaches a predetermined level. The biocide may be oxidizing and/or non-oxidizing.

Optical sensor fouling (ODL) can be attached to the specified flow cell to determine the nature/origin of the sediments, which are formed in the process stream.

Optical sensor fouling (ODL) can be attached to the specified flow cell to determine the nature/origin of the sediments, which are formed in the process stream.

In one of the embodiments of the present invention the method further includes: providing an optical sensor fouling, connected with the specified process stream; selection of fluid from the specified process stream in the specified optical sensor fouling; measurement and scaling of the optical sensor fouling; determining the type of sediments by correlation the formation of deposits in the specified optical sensor fouling with the specified microbiological activity, determined from Δ in the specified process flow; it is possible that the programming of the controller connected to the specified ODO and at least one probe RK, to add to the specified process flow of one or more chemical substances depending on correlation of the specified education otlozhenii the microbiological activity.

In another embodiment of the present invention, if this correlation shows that the formation of deposits on the optical sensor fouling is microbiological in nature, the chemicals contain a biocide. For example, if the ODO is the deposition and Δ high, then one way is to add biocide to the specified process flow to combat the formation and reduction of microbiological activity in the process stream. Biocides may be oxidizing and/or non-oxidizing.

In another embodiment of the present invention, if this correlation shows that the formation of deposits has necrobiology nature, the chemicals are chemical substances that regulate the formation of deposits. For example, if the ODO is the deposition and Δ low, then one of the ways is adding to the process flow of the chemical substances that regulate the formation of deposits, to combat the formation of deposits. There are different types of chemicals to control sediments, well-known specialist in the art; for example, substances that prevent the resin formation, which help prevent Deposit formation in the manufacture of paper, as well as polymers that regulate the formation of deposits.

D. MONITORING of BULK AND SURFACE MICROBIOLOGICAL ACTIVITY IN a PROCESS STREAM

Monitoring bulk microbiological activity can be undertaken in conjunction with monitoring of surface microbial activity. Method of measurement of bulk microbiological activity and surface microbiological activity in a process stream includes: (a) attaching the device to the specified process flow stream, with the specified device includes a flow cell provided with holes, where at least one opening is an inlet flow cells for sampling fluid from the specified process stream and at least one opening is an outlet for release of fluid from the specified flow cells, probe RK attached to one of these holes, perhaps the ORP probe attached to one of these holes, perhaps a cleansing device, attached to one of these holes, perhaps the first pipeline connected to the inlet hole of the flow-through cell, possibly, a second pipe connected to the outlet flow cells, possibly valve attached to the said flow cell; (b) sampling the fluid from the specified those is technological flow specified in the flow cell; (c) opening the valve of the specified device for the transmission of fluid at a specified flow cell; (d) at least one measurement of the concentration of the Republic of Kazakhstan in the specified process stream using the specified probe RK, and prior to each measurement the surface of the specified probe RK is not clear; (e) cleaning the surface of the specified probe RK; (f) at least one concentration measurement RK fluid inside the device, using the specified probe RK, possibly, prior to each measurement the surface of the specified probe RK is cleaned; (g) the valve closes the specified device prevent selection of the fluid at a specified flow cell; (h) at least one concentration measurement RK fluid inside the device, using the specified probe RK, and prior to each measurement the surface of the specified probe RK clean; (i) the dierence Δ between the readings obtained at stage (f) and phase (h), and the establishment of a correlation at least between the specified value Δ and the specified volume of microbiological activity in the specified process flow and (j) calculating the difference Δ between the readings obtained in stage (d) and stage (f), and the establishment of a correlation at least between the specified index Δ and specified by Ernesto microbiological activity in the specified process stream.

In another embodiment of the present invention, the monitoring carried out in such a way that the operator can toggle between the definition of bulk microbiological activity (Normal mode) and/or surface activity (Biofilms). On Fig shows a block diagram of one example implementation of this mechanism.

In another embodiment of the present invention the method also includes at least a single measurement of redox potential on stage (d), stage (f) and phase (h), where the ORP probe is not cleaned on stage (d), may be specified ORP probe is cleaned at stage (f) and the ORP probe is cleaned at stage (h)the possible addition of one or more oxidants to the specified process flow, if the values of the redox potential drops below a specified level, and the possible exception of the aforementioned results RK when calculating the value specified Δ if the specified value ORP falls below the specified level.

In another embodiment of the present invention in conjunction with the specified method can be used to monitor the formation of deposits from the process stream. More specifically, the method according to the present invention further includes: providing an optical sensor fouling, connected with the specified process stream; selection of fluid from the specified process stream specified on the optical sensor fouling; measurement of deposition using optical sensor fouling; determining the type of sediments by correlation the formation of deposits in the specified optical sensor fouling with the specified microbiological activity, determined from Δ in the specified process flow; may program the controller to add to the specified process flow of one or more chemical substances, depending on the correlation of the specified deposits microbiological activity.

The following examples are non-limiting examples of the present invention.

EXAMPLES

Example 1

The process stream selected from the flow cell through the first pipeline. The intake flow in a flow cell to regulate one or more valves. Drain connected to the first pipeline and one or more valves, prevents the return of the environment in the process stream or contributes to the control of clogging by solid particles that are present in the process stream. In terms of leakage flow position of the valve provides the transmission fluid in a flow cell. To the flow cell attached probe RK, probe ORP and purifying device (for example, the wiper). The fluid passed through the flow cell for analysis is.

Depending on the purpose of monitoring (monitoring volumetric or surface microbiological activity, or a combination of) the valve is installed in the open position or closed position to provide fluid in the flow cell and record the concentration of the Republic of Kazakhstan and/or the value of the AFP in accordance with one of the above protocols. Fluid passing through the flow cell, and exits through a drain. Fluid which enters the flow can be directed back into the process stream, for example in the case of paper machines. Figure 9 shows the layout of flow cell and the flow of the process stream through such arrangement of flow cells.

Process flow can also be attached sensor ODO. The flow direction in ODL regulate one or more valves. Figure 10 shows the layout of a flow cell with sensor ODO, and the flow of the process stream through such arrangement of flow cells and ODO.

Depending on the degree of microbiological activity and/or sediments in the process stream into the process stream can be introduced by suitable chemicals that can contribute to resolving problems. For example, the controller may be signaled to the pump, which operates with enoid, attached to the feeding mechanism.

Example 2

Side zipper process water of the paper making process used in the paper mill located in Germany, was passed through the device for monitoring (2 liters per second). On the factory produces coated and uncoated paper sheets that do not contain wood pulp, and for biological control use stable oxidant. The valve, located on the device for monitoring, closed and reopened after 60-minute intervals, stopping or resuming the flow in the flow cell device for monitoring. The values of AFP and LRC was measured in 10-minute intervals. The data recorded using the devices for measuring the redox potential and LRC were collected by a data logger and transferred to the web server, and then displayed on the web site. Data were downloaded from the web site and analyzed to determine the impact of a program of biological control and treatment conditions for microbial activity.

In this application example implementation of the present invention the method included the use of ODL to determine the nature/source of interfering deposits. For example, if deposits and activity is high, this means that deposits are likely to have a biological origin is selected. Conversely, if the amount of sediment high and low microbiological activity, it is unlikely that microorganisms are the cause of the formation of deposits, and this problem is due to other causes. The example shown on Fig.6 shows the effect of stopping the equipment in the ORP, microbiological activity and sediment (ODL) in stagnant process water. Microbiological activity was defined as Δ. The equipment was stopped on 4 August. Soon after stopping the observed sharp increase Δ, which coincided with a decrease in redox potential and increased fouling of the surface, measured using ODL. These data indicate that the program is based on the action of oxidants, acts inconsistent and does not allow adequate control of microbial growth and the formation of deposits during the specified event. The study of surface sediments under a microscope confirmed the presence of large concentrations of microorganisms, including filamentous bacteria.

Example 3

Side zipper process water used in the papermaking process at a paper mill in the United States, was passed through the device for monitoring (0.25 litres per second). The above factory often change the fiber content in the final product that has a significant impact on the done is the program of biological control. In particular, on the specified factory use composition Azoto, which increases the consumption of the halogen in the system process water. The valve, located on the device for monitoring, closed and reopened in 30-minute intervals, stopping or resuming the flow in the flow cell device for monitoring. The values of AFP and LRC was measured in 6-minute intervals. The data recorded using instruments to determine the redox potential and LRC were collected by a data logger or loaded into the computer using the software supplied with the device to be monitored.

Soon after you install a device to monitor directly observed process changes that affect the performance of the biological control program, based on measurements of redox potential, the degree of microbiological activity and fouling of the surface, measured by the JDS. The example shown in Fig.7 shows the effect of changes in the content of the fibers on the redox potential, microbial activity and sediments (ODL). Microbial activity was defined as LRC (% saturation), and a large difference between the original LRC in terms of the flow stream and LRC, measured in terms of stopping the flow, indicates higher microbial activity. These data indicate that the program is MMA, based on the action of oxidants, acts inconsistent and does not allow adequate control of microbial growth and the formation using the composition Azoto, requiring a large number of oxidants. Thus, to better control the formation of deposits during the production of this brand paper program should be modified.

Example 4

Using the dissolved oxygen sensor produces a continuous measurement of dissolved oxygen concentration. Monitoring program control by using PLC (programmable logic controller)that allows you to read and store the measured values LRC until the completion of the programming cycle. The PLC also allows you to control unit wiper, which produce clear screen sensor, and a mechanical ball valve, which stops the flow of water through the cell for analysis of samples.

The program provides two modes: the mode of measurement of bulk microbiological activity (GMA) and/or the mode of measurement of surface microbial activity (ACA). To meet program specific application in both modes use a set of three variables: X, Xt and Xti. More specifically, X represents the time the ball valve in open and closed States, expressed in minutes, Xt represents the number of readings LRC stored during the time X, and Xti represents the interval between readings LRC. When the ball valve in the open position and the flow of sample readings LRC should be stable, reflecting the current state of the source from which samples are taken. When the ball valve in the closed position and stop the flow of the sample stops the supply of dissolved oxygen in a closed flow-through cell that leads to its depletion by reaction with organic material.

In OHM mode, all values recorded directly after cleaning the probe. Values Δ represent a measure of microbial activity inside the sample, reflecting the consumption of dissolved oxygen during metabolism.

Mode PMA electrode is not clear within the first part of the cycle of valve opening. During this time on the surface of the electrode may be an accumulation of biofilm. Then the electrode is cleaned, and the difference indicates the amount of biofilm accumulated during the first part of the cycle. Then the ball valve is closed and take readings as well as in OHM mode.

Table 1
Dir is m measurement OHM
X=10; Xt=5
Time (min)SequenceEventReadingThe sample flow
00:00StartThe ball valve is openCourse
01:00Xti-01:00Clean
01:30Xti-00:30Read LRC1
03:002Xti-01:00Clean
03:302Xti-00:30Read LRC2
05:003Xti-01:00Clean
05:303Xti-00:30Read LRC3
07:00 4Xti-01:00Clean
07:304Xti-00:30Read LRC4
09:005Xti-01:00Clean
09:305Xti-00:30Read LRC5
10:005XtiThe ball valve is closedStop
11:006Xti-01:00Clean
11:306Xti-00:30Read LRC6
13:007Xti-01:00Clean
13:307Xti-00:30Read LRC7
15:008Xti-01:00Clearance
15:308Xti-00:30Read LRC8
17:009Xti-01:00Clean
17:309Xti-00:30Read LRC9
19:0010Xti-01:00Clean
19:3010Xti-00:30Read LRC10
20:0010XtiThe cycle is completed
MAX = the average of the readings 1-5
MIN = minimum readings 6-10
Activity:
OHM=MAX-MIN

Table 2
The measurement mode PMA (Readings 1-7) and the Mode of measurement OHM/td>
Time (min)SequenceEventReadingsThe sample flow
00:00StartThe ball valve is openCourse
04:30Xti-01:30Read LRC1
12:302XtiRead LRC2
18:003XtiRead LRC3
24:004XtiRead LRC4
30:005XtiRead LRC5
30:305Xti+0:30Cleaning twice
31:005Xti+1:00Sityva is their LRK 6
31:205Xti-01:20Read LRC7
The ball valve is closedStop
35:00X+(Xti-01:00)Clean
35:30X+(Xti-00:30)Read LRC8
41:00X+(2Xti-01:00)Clean
41:30X+(2Xti-00:30)Read LRC9
47:00X+(3Xtt-01:00)Clean
47:30X+(3Xti-00:30)Read LRC10
53:00X+(4Xti-01:00)Clean
53:30 X+(4Xti-00:30)Read LRC11
59:00X+(5Xti-01:00)Clean
59:30X+(5Xti-00:30)Read LRC12
60:002XThe cycle is completed
In MIN = Reading 5
In MACH = the Average of the readings 6 and 7
MIN = Minimal value of 8-12
Activity:
OHM=BMAX-MIN
ACA=WMAH-BMIN

1. Device for monitoring microbiological activity in a process stream of water, including:
(a) a flow cell provided with holes, where at least one opening is an inlet flow cells for sampling fluid from the specified process stream and at least one opening is an outlet for release of fluid from the specified PR is exact cell;
(b) probe RK attached to one of these holes;
(c) perhaps the ORP probe attached to one of these holes;
(d) a cleaning device attached to one of these holes;
(e) a first pipeline connected to the inlet hole of the flow cells;
(f) perhaps the second pipe attached to the outlet flow cells, and
(g) a valve attached to the specified flow cell.

2. The device according to claim 1, in which the specified purifying device is a wiper or brush.

3. The device according to claim 1, in which at least part of the specified probe RK and possibly probe ORP goes inside the specified flow cells.

4. The device according to claim 1, wherein said valve is attached to the specified first pipeline.

5. The device according to claim 1, wherein said valve is a ball valve and at the same time you may have specified the ball valve is a ball valve, actuated manually, an electric mechanism or a pneumatic mechanism.

6. The device according to claim 1, further including a controller that is in the message at least one of the following: probe ORP probe RK and purifying device.

7. The device according to claim 1, in which the specified flow cell SN is beena one or more partitions.

8. Method of monitoring the overall (total) microbiological activity of water in the process stream of water, including:
a. attaching the device to the process flow stream, with the specified device includes a flow cell provided with holes, where at least one opening is an inlet flow cells for sampling fluid from the specified process stream and at least one opening is an outlet for release of fluid from the specified flow cells; probe RK attached to one of these holes; perhaps the ORP probe attached to one of these holes; a cleaning device attached to one of these holes; a first piping connected to the inlet hole of the flow cells; it is possible that the second the tubing attached to the outlet flow cells, and the valve attached to the said flow cell;
b. the selection of fluid from the specified process flow specified in the flow cell;
c. open the valve of the specified device for the transmission of fluid at a specified flow cell;
d. at least a single measurement of the concentration of the Republic of Kazakhstan in the specified process stream using the specified probe RK, and before each and what limits the surface of the specified probe RK cleaned;
e. the closing of the valve device for terminating the selection of the fluid at a specified flow cell;
f. at least one concentration measurement RK fluid inside the device, using the specified probe RK, and prior to each measurement the surface of the specified probe RK cleanse;
g. the dierence Δ between the readings obtained in stage (d) and stage (f), and
h. definition of the overall (total) microbiological activity in the specified process flow calculated at the stage of the value Δ.

9. The method of claim 8, wherein said monitoring is produced continuously or periodically.

10. The method of claim 8, further comprising providing between stages (d) and (f) from 5 to 240 min time required for consumption of oxygen contained in the specified flow cell, one or more microbial species.

11. The method of claim 8, additionally comprising at least one measurement of redox potential on stage (d) and phase (f) and cleaning the surface of the ORP probe before each measurement; the possible addition of one or more oxidants in the specified process stream and the possible exception of the aforementioned results of measurements of RK based Δ when falling the specified value ORP below 100 MB.

12. The method according to claim 11, wherein said predetermined level of AFP is IU is approximately 100 MB.

13. The method according to claim 8, in which the value Δ determine, choosing the average measurement result of the RK on stage (d) and the minimum measurement result of the RK on the stage (f).

14. The method according to claim 8, in which the value Δ determined by choosing the highest measurement result of the RK on stage (d) and the minimum measurement result of the RK on the stage (f).

15. The method according to claim 8, in which the value Δ determine, choosing the last measurement result of the RK on stage (d) and the minimum measurement result of the RK on the stage (f).

16. The method of claim 8, further comprising a programmable controller, which is located in the message at least one of the following: probe ORP probe RK, purifying device or combination thereof.

17. The method according to item 16, wherein said controller receives input signals from the specified probe RK and performs the required Protocol that is programmed in the specified controller.

18. The method according to 17, wherein said controller is Web-based.

19. The method according to 17, wherein said desired Protocol includes alert the operator or person responsible for monitoring the process stream and processing the process stream.

20. The method of claim 8, wherein said process stream is a process stream from a process selected from the group comprised the soup from the paper making process, process cooling water, process food or beverage and process of recreational water use.

21. The method of claim 8, further comprising providing the optical sensor fouling, connected with the specified process stream; selection of fluid from the specified process stream in the specified optical sensor fouling; measurement and scaling of the optical sensor fouling; determining the type of sediments by correlation the formation of deposits in the specified optical sensor fouling with the specified microbiological activity, determined from Δ in the specified process flow; it is possible that the programming of the controller connected to the specified ODO and at least one probe RK, to add to the specified process flow of one or more chemical substances, depending on the correlation of the specified deposits microbiological activity.

22. The method according to item 21, in which these chemicals are biocides when reading the specified correlation, which indicated the formation of deposits is microbiological in nature, with the specified biocide may be oxidizing and/or non-oxidizing.

23. The method according to item 21, in which these chemicals are chemical substances is TBA, governing the formation of deposits when reading the specified correlation, which indicated the formation of deposits has necrobiology nature.

24. The method of claim 8, further comprising providing a response to the degree of bulk (total) microbiological activity in the specified process flow; when this is possible, the Protocol includes adding an effective amount of biocide that allows to reduce the level of microbiological activity to the desired index.

25. The method according to paragraph 24, where the specified process stream is a process flow of the papermaking process and the specified biocide chosen from the group consisting of the following compounds: isothiazoline; glutaraldehyde; dibromonitrilopropionamide; carbamate; Quaternary ammonium compounds; sodium hypochlorite; chlorine dioxide; peracetic acid; ozone; chloramines; promulgate; bromchloromethane; dichlorodimethylsilane; monochloramine; sodium hypochlorite in combination with ammonium salts and stabilizers, including dimethylhydantoin, amino acid, cyanuric acid, succinimide, urea, and combinations of the listed compounds.

26. Method of monitoring surface microbiological activity in a process stream of water, which includes:
a. attach the mouth of the STS to the process flow stream, moreover, the specified device includes a flow cell provided with holes, where at least one opening is an inlet flow cells for sampling fluid from the specified process stream, and at least one opening is an outlet for release of fluid from the specified flow cells; probe RK attached to one of these holes; perhaps the ORP probe attached to one of these holes; a cleaning device attached to one of these holes; a first piping connected to the inlet hole of the flow cells; possibly, a second pipe connected to the exhaust the opening of the flow cell; and a valve attached to the said flow cell;
b. the selection of fluid from the specified process flow specified in the flow cell;
c. open the valve of the specified device for the transmission of fluid at a specified flow cell;
d. at least a single measurement of the concentration of the Republic of Kazakhstan in the specified process stream using the specified probe ROK and the specified probe RK is not cleaned before each measurement;
e. surface cleaning of the specified probe RK;
f. at least one concentration measurement RK fluid in Utri specified device with the specified probe ROK and perhaps before each measurement the surface of the specified probe RK cleanse;
g. the dierence Δ between the readings obtained in stage (d) and stage (f), and
h. determination of surface microbial activity in the specified process flow calculated at stage (g) the value Δ.

27. The method according to p, additionally comprising at least one measurement of redox potential on stage (d) and stage (f), wherein the ORP probe is not cleaned on stage (d), and, possibly, the ORP probe is cleaned at stage (f)the possible addition of one or more oxidants in the specified process stream, if the value of the redox potential is below a specified level; and a possible exception to the above measurement results RK based Δ when falling the specified value ORP below 100 MB.

28. The method according to p, in which the surface of the specified probe RK cleaned twice using cleaning equipment, which includes the wiper.

29. The method according to p, wherein said monitoring is produced continuously.

30. The method according to p, including additional software before carrying out stage (d) from 5 to 240 min time required for the accumulation of biofilms.

31. The method according to p, which is Δ determined by choosing the smallest measurement result of the RK on stage (d), and the average measurement result of the RK on the stage (f).

32. The way is about p, which is Δ determined by choosing the smallest measurement result of the RK on stage (d) and the highest measurement result of the RK on the stage (f).

33. The method according to p, which is Δ determine, choosing the last measurement result of the RK on stage (d) and the highest measurement result of the RK on the stage (f).

34. The method according to p, further comprising a programmable controller, which is located in the message at least one of the following: probe RK, ORP probe, purifying device or combination thereof.

35. The method according to clause 34, wherein said controller receives input signals from the specified probe ROK and the specified probe ORP, performs the required Protocol that is programmed in the specified controller.

36. The method according to clause 34, wherein said controller is Web-based.

37. The method according to p, wherein said Protocol includes a warning to the operator or person responsible for monitoring the process stream and processing the process stream.

38. The method according to p, further comprising providing the optical sensor fouling, connected with the specified process stream; selection of fluid from the specified process stream in the specified optical sensor fouling; measurement and scaling of the optical sensor is Brytania; determining the type of sediments by correlation the formation of deposits in the specified optical sensor fouling with the specified microbiological activity, determined from Δ in the specified process flow; it is possible that the programming of the controller connected to the specified ODO and at least one probe RK, to add to the specified process flow of one or more chemical substances, depending on the correlation of the specified deposits microbiological activity.

39. The method according to § 38, in which these chemicals are biocides when reading the specified correlation, which indicated the formation of deposits is microbiological in nature, with the specified biocide may be oxidizing and/or non-oxidizing.

40. The method according to p, further comprising providing a response to the degree of surface microbial activity in the specified process flow; when this is possible, the Protocol includes adding an effective amount of biocide that allows to reduce the level of microbiological activity to the desired index.

41. The method according to p where the specified process stream is a process flow of the papermaking process and the specified biocide chosen from the group consisting of the following soybeans is ineni: isothiazoline; glutaraldehyde; dibromonitrilopropionamide; carbamate; Quaternary ammonium compounds; sodium hypochlorite; chlorine dioxide; peracetic acid; ozone; chloramines; promulgate; bromchloromethane; dichlorodimethylsilane; monochloramine; sodium hypochlorite in combination with ammonium salts and stabilizers, including dimethylhydantoin, amino acid, cyanuric acid, succinimide, urea, and combinations of the listed compounds.

42. The method according to p, wherein said monitoring is produced continuously or periodically.

43. Method of monitoring bulk microbiological activity and surface microbiological activity in a process stream of water, including:
a. attaching the device to the specified process flow stream, with the specified device includes a flow cell provided with holes, where at least one opening is an inlet flow cells for sampling fluid from the specified process stream and at least one opening is an outlet for release of fluid from the specified flow cells, probe RK attached to one of these holes, perhaps the ORP probe attached to one of these holes, a cleaning device attached to gnome of these holes, the first pipeline connected to the inlet hole of the flow-through cell, possibly, a second pipe connected to the outlet of a flow cell, and a valve attached to the said flow cell;
b. the selection of fluid from the specified process flow specified in the flow cell;
c. open the valve of the specified device for the transmission of fluid at a specified flow cell;
d. at least a single measurement of the concentration of the Republic of Kazakhstan in the specified process stream using the specified probe RK, and prior to each measurement the surface of the specified probe RK is not clear;
e. surface cleaning of the specified probe RK;
f. at least one concentration measurement RK fluid inside the device, using the specified probe RK, possibly, prior to each measurement the surface of the specified probe RK cleanse;
g. the valve closes the specified device to stop the selection of the fluid at a specified flow cell;
h. at least one concentration measurement RK fluid inside the device, using the specified probe RK, and prior to each measurement the surface of the specified probe RK cleanse;
i. the dierence Δ between the readings obtained at stage (f) and phase (h), and the definition of the lines of microbiological activity in the specified process flow according to the calculated difference Δ between readings, obtained in stage (f) and stage (h).
j. the dierence Δ between the readings obtained in stage (d) and stage (f), and determination of surface microbial activity in the specified process flow according to the calculated difference Δ between the readings obtained at stage (f) and stage (h).

44. The method according to item 43, further comprising providing the optical sensor fouling, connected with the specified process stream; selection of fluid from the specified process stream in the specified optical sensor fouling; measurement and scaling of the optical sensor fouling; determining the type of sediments by correlation the formation of deposits in the specified optical sensor fouling with the specified microbiological activity, determined from Δ on stage (i) and/or stage (j) in the process stream; it is possible that the programming of the controller connected to the specified ODO and at least one probe RK, to add to the specified process flow of one or more chemical substances, depending on the correlation of the specified deposits microbiological activity.

45. The method according to item 43, additionally comprising at least one measurement of redox potential on stage (d), stage (f) and phase (h), wherein the probe ORP is not clear on what Tadei (d), and, perhaps, the ORP probe is cleaned at stage (f), and the ORP probe is cleaned at stage (h)the possible addition of one or more oxidants in the specified process stream and the possible exception of the aforementioned results of measurements of RK based Δ when falling the specified value ORP below 100 mV.



 

Same patents:

FIELD: biotechnologies.

SUBSTANCE: method includes the following stages: contact of a sample with a source of nutrition for cells, containing antioxidant, representing pyroracemic acid or its salt, and an inhibitor of cell proliferation, which is selected from ciprofloxacin and cefalexin; contact of the specified sample with fluorescent-marked oligonucleotide probes, capable of specific hybridisation at least with one section of ribosomal nucleic acids, which belong to microorganisms of Legionella pneumophila kind and type; and detection and quantitative determination of a fluorescent signal.

EFFECT: provided method and set make it possible to more accurately and reliably detect and calculate viable microorganisms of Legionella pneumophila type, having excluded natural fluorescence of microorganisms from calculation.

6 cl, 2 dwg, 6 tbl, 2 ex

FIELD: medicine, pharmaceutics.

SUBSTANCE: method under the invention provides detoxification and polymerisation of antibiotics in 0.15±0.05% glutaric aldehyde at 38-40°C for 3-5 days, and then in 0.1% alkyl dimethyl benzyl ammonium chloride at 38-40°C for 3-5 days.

EFFECT: method provides effective bactericidal, virusocidal and fungicidal action of antibiotics and enables extending their therapeutic spectrum of application.

1 tbl, 4 ex

FIELD: medicine, pharmaceutics.

SUBSTANCE: method under the invention involves detoxification and polymerisation of the tested antibiotics 150-200 mg/ml at first in 0.15±0.05% glutaric aldehyde at 38-40°C for 2-3 days, then in 0.1% aethonium or 0.1% alkyl dimethyl benzyl ammonium or 0.1% Biopague D at 38-40°C for 2-3 days.

EFFECT: use of the method provides 1,5-2,0 times higher activity of the antibiotic as compared to commercial preparations and ensures the bactericidal efficacy to antibiotic-resistant Ecoli.

2 ex

FIELD: biotechnologies.

SUBSTANCE: thermostatting of a biocatalyst is carried out on the basis of immobilised microbial cells and a non-innoculated carrier within the biocatalyst, as well as infrared scanning of the biocatalyst surface and the carrier with the help of a highly sensitive infrared chamber, and production of thermal characteristics of the biocatalyst, such as distribution of temperatures on its surface and difference of temperatures between the surface of the biocatalyst and the non-innoculated carrier. Distribution of temperatures makes it possible to control homogeneity of activity distribution on the biocatalyst surface. The difference of temperatures is used to determine intensity of adsorption and metabolic activity of fixed bacterial cells. A plant for detection of efficiency of adsorption immobilisation of microorganisms and monitoring of functional condition of biocatalysts includes an infrared chamber fixed on a tripod, and connected with a computer, and a heat-insulating box with a hole on top, closed with a cover, which makes it possible to minimise oscillations of ambient temperature down to ±1°C/hr and reduces impact of the infrared chamber at analysis results.

EFFECT: reduced time of analysis performance, higher efficiency and profitability of biological methods of chemical compounds transformation and biological utilisation of hazardous substances.

6 cl, 2 dwg, 3 tbl, 6 ex

FIELD: medicine.

SUBSTANCE: invention refers to medical microbiology and concerns differentiation of toxigenic and non-toxigenic strains of cholera vibrios serogroup 0139. The described method involves preparing a synthetic medium 100 ml by using weights of: sodium chloride 0.5 g, agar 2 g, bromthymol blue 0.002 g; then the weight ingredients are dissolved in 0.01 M tris HCl buffer 100 ml, pH 7.8 and boiled for 30 minutes; the prepared medium is added with a substrate in the form of 1% aqueous sodium dodecyl sulphate (SDS) to the final concentration of 0.1% in the medium; it is followed by loop inoculation of the prepared medium on the analysed culture and incubation for 24-48 hours; the results are considered by the presence of milky-white areas 2-10 mm surrounding the inoculations on the agar sectors; the presence of those makes the strain to be referred to as toxicogenic (ctx+ tcp-), while the absence of those shows that the strain is non-toxicogenic (ctx+ tcp-).

EFFECT: method is simple and visual and may be used for determining the epidemic relevance of the strains of Vibrio Cholerae 0138 serogroup.

3 tbl, 3 ex

FIELD: medicine, pharmaceutics.

SUBSTANCE: invention relates to field of medicine, namely to microbiology and is intended for increasing efficiency of microbiological diagnostics of staphylococcal infections. Method of isolating uncultivable staphylococcus bacteria includes cultivation of studied material on beef-extract agar for 24 hours at temperature 37°C. After that, grown bacterial culture is kept at temperature +4°C for 48 hours.

EFFECT: method makes it possible to increase efficiency of isolation of causative agents of staphylococcal infections, reduce number of false negative results of analyses and ensure carrying out of anti-epidemic actions in foci of staphylococcal infections more fully.

2 ex

FIELD: chemistry.

SUBSTANCE: culture medium for determining drug susceptibility of Mycobacterium tuberculosis to four main drugs - streptomycin, isoniazid, rifampin and ethambutol contains: 4.7 g dry Middlebrook 7H9 broth, 1.25 g microbioligical agar-agar, 1.25 g pancreatic digest of casein, 900 ml distilled water and 100 ml growth additive OADC. The additive contains, g/l: sodium chloride 8.5, bovine albumin (fraction 5) 50.0, glucose 20.0, catalase 0.03 and oleic acid 0.6 ml/l.

EFFECT: invention enables to obtain analysis results faster and ensures easy visual reading of results.

4 tbl

FIELD: medicine.

SUBSTANCE: claimed is method of detecting antibodies to Mycobacterium leprae (M.leprae) on solid carrier. Places of application of components of immunologic reaction in ELISA on solid carrier (fluoroplastic tape) are sensitised with antigen from ultrasound disintegrate (sonicate) of M.leprae in dose 5 mcg/ml in volume 20 mcl for each sample of patients' blood serum. After that immunoperoxidase conjugate against human IgG is applied in the same volume on the places of previous location of reaction components. Unbound antigen from sensitised tape is removed with buffer solution (BPST) after each stage of reaction. Reaction results are estimated visually by difference in substrate mixture colouring.

EFFECT: simplification of method of detection of antibodies to Mleprae due to application in ELISA of solid carrier-fluoroplastic tape.

2 ex

FIELD: medicine.

SUBSTANCE: in in vitro conditions, imitating digestion process in humans quantities of live microorganisms at the beginning and at the end of experiment are determined and their numeric values are compared. Conclusion is made on the basis of the results of comparison after incubation of probiotic microorganisms during 4 h in acidic model medium with acidin-pepsin by inoculation of microorganism suspension on dense nutritional medium. Grown colonies are counted and number of viable microorganisms is determined. Remaining suspension is separated from incubation medium, alkaline model medium with pansinorm forte 20000 is added to sediment in volume, analogous to volume of acidic model medium. Sediment is resuspended and suspension is incubatred during 12 hours, remaining number of viable microorganisms is determined by inoculation on a dense nutritional medium and counting grown colonies.

EFFECT: increased efficiency and accuracy of method and possible therapeutic-preventive efficiency of medication, administered to patient with intestinal disbacteriosis.

5 cl, 4 ex

FIELD: medicine.

SUBSTANCE: diagnostic technique for microsporia in implemented by featuring clinical manifestations of the process, studying macro-and microphology of the culture. The presence of 1-2 centres of size 0.5-1.5 cm primarily localised on the scalp and the presence of two individual cell macroconidia in the form of a horseshoe with a thick pitted wall enable diagnosing microsporia caused Microsporum cams var.distortum. By using the complex of anamnestic, clinical, microscopic and morpho-biological signs, the invention allows establishing a precise etiological diagnosis of a microsporia agent - M canis var. distortum that is important in the process of the antimycotic therapy.

EFFECT: use of the technique shall enable improving diagnosing, prediction of the clinical course of microsporia, duration of the disease and treatment.

1 tbl

FIELD: biotechnologies.

SUBSTANCE: method includes the following stages: contact of a sample with a source of nutrition for cells, containing antioxidant, representing pyroracemic acid or its salt, and an inhibitor of cell proliferation, which is selected from ciprofloxacin and cefalexin; contact of the specified sample with fluorescent-marked oligonucleotide probes, capable of specific hybridisation at least with one section of ribosomal nucleic acids, which belong to microorganisms of Legionella pneumophila kind and type; and detection and quantitative determination of a fluorescent signal.

EFFECT: provided method and set make it possible to more accurately and reliably detect and calculate viable microorganisms of Legionella pneumophila type, having excluded natural fluorescence of microorganisms from calculation.

6 cl, 2 dwg, 6 tbl, 2 ex

FIELD: chemistry.

SUBSTANCE: present invention relates to chemical industry and agriculture and is a plant growth stimulating agent.

EFFECT: invention enables to produce an agent capable of regulating plant growth or differentiation.

16 cl, 22 ex, 11 dwg

FIELD: medicine.

SUBSTANCE: method for prediction of bacterial translocation into blood in generalised periodontitis provides recovery of symbiont strains from gingival pocket biocenosis and comparison of their hemolytic activity (HA), antilysozyme activity (ALA) and growth.

EFFECT: invention enables planning and implementing targeted preventive remedial measures in generalised periodontitis.

3 dwg, 5 tbl, 2 ex

FIELD: medicine.

SUBSTANCE: tear is taken by sterile disks made of a porous material to form a microbial lawn and to determine lysocyme activity by lysocyme diffusion from the disk into the microbial lawn. It is followed by incubation in a thermostat for at least 24 hours. A zone of microbial growth retardation is determined by measuring a diameter of the zone of microbial growth retardation. The diameter of the zone of microbial growth retardation in healthy infants is 28-30 mm, while the diameter of the zone of microbial growth retardation in infants suffering inflammatory eye diseases is 20-24 mm.

EFFECT: method enables determining lachrymal lysocyme activity in infants, including newborns.

2 ex

FIELD: medicine.

SUBSTANCE: antioxidant activity of a broth culture of microorganisms or a cell suspension of microorganisms grown on a solid nutrient medium is evaluated. An oxidable medium is presented by a lecithin solution, while peroxide initiators are as follows: Staphylococcus aureus cells, ascorbic acid, ferric (II) ions (in the form of an aqueous solution of ferric sulphate). Antioxidant activity is evaluated by an ability to inhibit lipid peroxidation by adding concentrated orthophosphoric acid, and 1% 2-thiobarbituric acid in dimethyl sulphoxide or ethanol (1:1). The stained complex is extracted in n-butanol, and after a butanol phase separated, it is spectrophotometered with regard to transmission unit at 550 nm wherein a reference tray is a tray with n-butanol. It is combined with preparing two controls wherein test tubes containing an oxidation substrate - lecithin are added with known antioxidant activity, while in the other one initiated peroxidation is enabled with no antioxidant added.

EFFECT: derived values of the antioxidant status are calculated in units of antioxidant activity.

1 ex

FIELD: medicine.

SUBSTANCE: Yersinia pseudotuberculosis 634 bacterial strain is recovered from a patient suffering pseudotuberculosis, deposited in the collection of the Russian Science and Research Antiplague Institute Microbe, No. KM 214 and is applicable as a test strain for differentiation of Yersinia pseudotuberculosis bacteria genetic group IA. The characteristic of the strain is the content of chromosomal gene of the Y.pseudotuberculosis YPMa/YPMc (urtA/C) superantigen and the YAPI (pilPQ) Yersinia adhesive pathogenicity island gene determined by PCR method.

EFFECT: invention shall enable epidemiologic study of sporadic and group cases of pseudotuberculosis: specifying an infection source in a hotbed of the disease and identifying transmission factors, as well as monitoring strain circulation at various territories.

2 dwg, 1 tbl

FIELD: medicine.

SUBSTANCE: Yersinia pseudotuberculosis 413 bacterial strain is recovered from a patient suffering pseudotuberculosis, deposited in the collection of the Russian Science and Research Antiplague Institute Microbe, No. KM 211 and is applicable as a test strain for differentiation of Yersinia pseudotuberculosis bacteria genetic group I. The characteristic of the strain is the content of chromosomal gene of the Y.pseudotuberculosis YPMa/YPMc (urtA/C) superantigen and the YAPI (pilPQ) Yersinia adhesive pathogenicity island gene, the pVM 82 plasmid of molecular weight 82 MDa determined by PCR method.

EFFECT: invention shall enable epidemiologic study of sporadic and group cases of pseudotuberculosis, specifying an infection source in a hotbed of the disease and identifying transmission factors, as well as monitoring strain circulation at various territories.

2 dwg, 1 tbl

FIELD: medicine.

SUBSTANCE: Yersinia pseudotuberculosis 563 bacterial strain is recovered from a patient suffering pseudotuberculosis, deposited in the collection of the Russian Science and Research Antiplague Institute Microbe, No. KM 212 and is applicable as a test strain for differentiation of Yersinia pseudotuberculosis bacteria genetic group IIA. The characteristic of the strain is the content of chromosomal gene of the Y. pseudotuberculosis YPMa/YPMc (urtA/C) superantigen determined by PCR method.

EFFECT: invention shall enable epidemiologic study of sporadic and group cases of pseudotuberculosis: specifying an infection source in a hotbed of the disease and identifying transmission factors, as well as monitoring strain circulation at various territories.

2 dwg, 1 tbl

FIELD: medicine.

SUBSTANCE: Yersinia pseudotuberculosis 1068 bacterial strain is recovered from a patient suffering pseudotuberculosis, deposited in the collection of the Russian Science and Research Antiplague Institute Microbe, No. KM 213 and is applicable as a test strain for differentiation of Yersinia pseudotuberculosis bacteria genetic group II. The characteristic of the strain is the content of chromosomal gene of the Y.pseudotuberculosis YPMa/YPMc (urtA/C) superantigen and the pVM 82 (dotO) plasmid of molecular weight 82MDa determined by PCR method.

EFFECT: invention shall enable epidemiologic study of sporadic and group cases of pseudotuberculosis: specifying an infection source in a hotbed of the disease and identifying transmission factors, as well as monitoring strain circulation at various territories.

2 dwg, 1 tbl

FIELD: medicine.

SUBSTANCE: sample from the upper airways contacts with a test strip. The test strip contains at least one wide-spectrum indicator which demonstrates a first spectral reaction in the presence of bacteria and a second spectral reaction in the presence of viruses. The wide-spectrum indicator is N-phenolate betaine. Besides, the test strip contains a mesh which comprises one differentiating indicator wherein the mesh demonstrates a third spectral reaction in the presence of one type of microorganisms and a fourth spectral reaction in the presence of the other type of microorganisms.

EFFECT: use of the declared method enables the rapid and easy assessment of the presence of viruses and bacteria in the test sample by the spectral indicator reaction.

7 ex, 18 tbl, 1 dwg

FIELD: biology.

SUBSTANCE: invention is designated for biotesting water and aqueous extract samples. Daphnia are immersed firstly in solution or water for culturing to be tested and then daphnia are transferred into lightproof chamber with outlet hole for culturing and this chamber is immersed into water. Time passing out daphnia from the testing chamber to light is measured and toxicity of analyzed solution is estimated by difference time in daphnia passing out. Method allows carrying out the rapid control of water and aqueous extracts toxicity in laboratory and field conditions.

EFFECT: improved method for biotesting.

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