Method of predicting severity of postoperative period course in patients with calculous cholecystitis
SUBSTANCE: in order to predict severity of postoperative period course in patients with calculous cholecystitis level of hormone grelin is determined in patients' blood serum before operation. If value of grelin level is 0.8-1.5 ng/ml mild course of postoperative period is predicted, if value of grelin level is >1.5-10.0 ng/ml course of medium severity is predicted. And if grelin level in blood serum increases to >10.0 ng/ml, severe course of postoperative period is predicted.
EFFECT: method makes it possible to increase accuracy of prediction of postoperative period course severity in patients with calculous cholecyctitis in cholecystectomy in order to perform treatment correction.
The invention relates to medicine, in particular to surgery, and for forecasting the severity of the postoperative period in patients with calculous cholecystitis during cholecystectomy.
Known methods of predicting the severity of the postoperative period in patients with calculous cholecystitis during cholecystectomy - neutrophilic leukocytosis with shift, accelerated erythrocyte sedimentation rate, hyperthermia. However, temperature increase, the increase in the number of leukocytes, accelerated erythrocyte sedimentation rate may be logged in the absence of systemic inflammation that prevents the use of data from clinical and laboratory characteristics for predicting the severity of the postoperative period in patients with calculous cholecystitis (Ilchenko A.A. Cholelithiasis. M: Anarcharsis, 2004 - 200 C.). Widespread integral scale (APACHE II and others) have low specificity in terms of individual prediction.
The closest analogue of the proposed method is the determination of C-reactive protein - PSA in the blood (Warworth et al. Clin. Chim. Acta. - 1984 - Vol.138. - P.69-71).
The disadvantage of this method is that the increase in the level of PSA in the blood is not specific, may be due to atherosclerosis, inflammation, necrosis, i.e. does not always provide objective information about the severity of the postoperative period. Furthermore, it is not the strict compliance to the severity of the inflammatory process and the level of PSA (Byszewski A.Ş, Arsenev O.A. Biochemistry for the doctor. Ekaterinburg, Ural worker", 1994 - S-332).
Effect: improve the accuracy of predicting the severity of the postoperative period in patients with calculous cholecystitis during cholecystectomy for correction treatment.
The result is achieved by defining in the serum of patients with calculous cholecystitis before cholecystectomy levels of the hormone ghrelin. Ghrelin is secreted by the endocrine cells of the stomach, hypothalamus and other organs. To date, proved the breadth of his actions. It has powerful anti-inflammatory effect through the inhibition of production of Pro-inflammatory cytokines, increase the percentage of activated T-helper cells, enhances apoptosis of lymphocytes and exerts immunomodulatory, anti-inflammatory, regenerative action (Kojima m, Hosoda h, Date y et al. Ghrelin is a growth-hormone-releasing airport ACY-lated peptide from stomach. // Nature. - 1999. - Vol.402. - P.656-660. Wasseem T., Duxbury M, Ito H, Ashley SW, Robinson MK Exogenous ghrelin modulates the release of proinflammatory and anti-inflammatory cytokines in LPS-stimulated macrophages through distinct signaling pathways. // Surgery 2008 - Vol.143. - P.334-342). Proven involvement of this hormone in the regulation of energy, carbohydrate, fat, lipid metabolism. Ghrelin stimulates the secretion of hormones by the pituitary gland, mainly, somatotropic, thereby improving the processes of regeneration. His role in the rehabilitation of the sick is x after surgical stress is high. In the available literature there is no work about the use of ghrelin to predict the severity paleodrainage period in patients with calculous cholecystitis during cholecystectomy.
The method is as follows. Before surgery in patients with calculous cholecystitis collect blood using venipuncture, blood is centrifuged, get the serum and in serum by ELISA to determine the levels of ghrelin (J. Fuglsang, Skjaebaek C., Espelund U, Frystyk J, Fisker S, Flyvbjerg A, Ovesen P: Ghrelin and its relationship to growth hormones during normal pregnancy. // Clin Endocrinol (Oxf) 2005, Vol.62, No. 5. - P.554-559). Normal level fluctuations in healthy minor: 0.5 to 0.7 ng/ml, because it is involved in maintaining homeostasis.
Ghrelin levels in serum from 0.8 to 1.5 ng/ml in patients with calculous cholecystitis before surgery suggests that postoperative period after cholecystectomy will have have a mild case, without complications. Ghrelin levels in serum >of 1.5 to 10.0 ng/ml in patients with calculous cholecystitis before surgery suggests that postoperative period after cholecystectomy will be moderate. Ghrelin levels in serum >of 10.0 and higher in patients with calculous cholecystitis before surgery suggests that postoperative period after cholecystectomy will have heavy during the s and should conduct timely adjustment of drug therapy. The study of ghrelin levels in serum of patients with calculous cholecystitis allows us to predict the severity of the postoperative period after cholecystectomy.
Examples of specific performance
Example 1. Patient H., 62 years old, suffering from cholelithiasis within three years. Bothered by frequent pain in the right hypochondrium. Hospitalized in an emergency in connection with the attack "biliary colic" after 9 hours from the beginning of the attack. A state of moderate severity. Skin subcarina. Tongue coated white bloom. The abdomen is not swollen, in the act of breathing is involved. Palpation of severe pain and a small defense in right hypochondrium. The gall bladder is not palpated. Ghrelin levels in the blood - to 20.6 ng/ml (normal 0.5-0.7 ng/ml), leukocytes of 8.90×109l, PSA 0,96 mg/l (normal 0-4,0 mg/l). Operated in an emergency after 3.5 hours from receipt due to the ineffectiveness of conservative therapy. On the basis of high hyperprolinemia predicted severe postoperative period, and therefore before the operation started antibiotics (Cefotaxime intravenously) and detoxication therapy (isotonic sodium chloride 500 ml potassium chloride 10% 5 ml magnesium sulfate 25% 5 ml intravenous drip. When laparoscopy: the liver is enlarged, the capsule uneven, dull. In the region of the STI gallbladder dense infiltrate. Gall bladder large, tense, partly situated intrahepatic. Wall thick, swollen, slimy in places black. When the puncture produced thick bile and pus. Performed cholecystectomy. In the neck of the bladder two stones up to 6 mm, obtenerse cystic duct. Histological examination in the mucosa of the gallbladder cholesterosis, leukocytic infiltration, foci of necrosis and hemorrhage in the wall. Conclusion: acute phlegmons-gangrenous cholecystitis. The patient was transferred to the intensive care unit. Postoperative period was hard: within four days of continued fever up to 38°C, unstable hemodynamics: fluctuations AD from 100/60 to 180/100 mm Hg; appeared polytopia arrythmia. Alat increased to 68 IU/l (normal range 0-35 IU/l)AST to 71 IU/l (normal up to 45 IU/l). Conducted antibacterial therapy - Cefotaxime 1,0/day intravenously for 5 days. Conducted infusion therapy. For prevention of thromboembolic complications immediately after surgery was used heparin subcutaneously to 4 days. Wound healing by primary intention. The patient was discharged on the 9th day.
Example 2. Patient P., aged 57, began to notice pain in the right hypochondrium in the month. Sonography revealed multiple gallstones, thickened walls. Blood tests: erythrocyte sedimentation rate 5 mm/h, lake. 8×109l, PSA 0,85 mg/who Made planned laparoscopic cholecystectomy. Diagnosis after surgery: cholelithiasis, chronic calculous cholecystitis. Histologically, the wall of the gallbladder lymphoid infiltration, fibrosis. Histopathological conclusion: chronic cholecystitis. Ghrelin levels before surgery: 0.9 ng/ml, i.e. has a favorable prognosis of postoperative period. Conducted antibiotic prophylaxis with Ceftriaxone 1.0/2 times - before and after surgery. Histology: lymphoid infiltration of the gallbladder wall. The postoperative period was uneventful. Temperature is normal. Leukocytes of 8.90×109l, Alat before and after surgery, respectively 20 IU/l, 22 IU/l, AST - 19 IU/l and 23 IU/L. Healing by primary intention. Discharged on day 6.
Example 3. Patient B., 52 years old, was hospitalized in the emergency order after 5 hours from the beginning of the attack of biliary colic with complaints of pain in the right hypochondrium, nausea, retching and vomiting, weakness. Sonography during preventive examination a year ago were found gallstones. Pain in hypochondrium didn't bother. When receiving a satisfactory condition. Tongue moist, coated with white bloom. The abdomen is not swollen, soft, painful to palpation in the right upper quadrant. The gall bladder is not palpated. The liver is not enlarged. Leukocytes of 7.6×109/l; ESR 25 mm/hour, Alat 24,0 IU/l, AST 15.0 M is/l; PSA 2.0 mg/l ghrelin Levels 9.0 ng/ml. Operated in an emergency. Gallbladder in inflammatory growths. In the gallbladder thick bile and stone with a diameter of 10 mm Cystic duct tortuous lumen to 3 mm Pathomorphological moderate sclerosis of the gallbladder wall, cholesterosis its walls and lymphoid and neutrophilic infiltration. Conclusion: acute exacerbation of chronic cholecystitis. Given the moderate increase in the concentration of ghrelin in serum, predicted postoperative period after cholecystectomy moderate, and that was confirmed. Temperature rose to 37.5°C, held for three days. Was expressed pain syndrome. The ECG recorded ventricular premature beats. Showed cytolytic syndrome: ALT 53,7 IU/l, ACT 47,9 IU/L. Held antibiotic cephalosporins. Introduced Ketonal 2 ml 2 times a day - two days. Introduced drip polarizing a mixture of glucose, insulin, potassium and saline, potassium chloride, sulphate of magnesia. Postoperative wound healing by primary intention. Discharged on day 7.
Example 4. Patient B., aged 45, is delivered to the surgical Department with complaints of pain in the right hypochondrium, nausea, weakness. The patient considers himself about two years, when there were recurrent pain in the right hypochondrium. Lately there is dust pains became more frequent. Made an ultrasound of the liver, gallbladder, pancreas and diagnosed with calculous cholecystitis. Yesterday after eating fatty and spicy foods pain in the right hypochondrium became intense. The condition is satisfactory. High power. The skin is clean, normal color. In pulmonary pathology. Heart sounds clean, rhythmic. HELL 120/80 mm Hg white furred Tongue, wet. The abdomen is not swollen, painful in the right hypochondrium. The gall bladder is not palpated. Border of the liver in norm. ESR 26 mm/hour, leukocytosis of 5.1×109/l; p 6%, S. 37%, l 50%m 7%. ALT 25 IU/l, ACT 27 IU/l Fibrinogen 2,63 g/l (normal range 2,0-4,0 g/l), prothrombin time of 11.8 seconds (normal 11-16 sec). Ghrelin levels were 1.5 ng/ml Given a slight increase in blood levels of ghrelin, predicted a slight postoperative period after cholecystectomy. So I spent the antibiotic Cefotaxime 1,0 intravenously before and after surgery. The postoperative course was easy. There were no complications. Subfebrile temperature was in the first day, then normal. From analgesics (ketonala) the patient refused. Postoperative wound healing by primary intention. Discharged on the 6th day in good condition. Pathomorphological - in the wall of lymphoid infiltration, fibrosis and in some places boundary distance of leukocytes in the blood vessels. Conclusion: the exacerbation of XP the technical cholecystitis.
Prediction of the severity of the postoperative period after cholecystectomy was performed in 25 patients with calculous cholecystitis. Controlled health patients operated, temperature, laboratory tests of inflammation, wound healing, hemodynamics, the presence of complications of the operation, the occurrence of cytolytic syndrome, signs of acute Bilyasuvarskogo pancreatitis. Ghrelin levels up to 1.5 ng/ml indicates that the post-operative period will leak easily, >1,5-10,0 ng/ml will be moderate, >of 10.0 ng/ml postoperative period heavy.
The value of the proposed method is that it allows us to predict the postoperative period in patients with calculous cholecystitis before cholecystectomy, to prepare for possible complications, to solve practical problems will require antibiotic therapy, or can be limited to the antibiotic, plan detoxication therapy, prevention of DIC, time to adjust therapy.
A method for predicting the severity of the postoperative period in patients with calculous cholecystitis by examining the blood, characterized in that before surgery in patients taken from a vein blood and serum to determine the levels of the hormone ghrelin, and at the value level, the ghrelin of 0.8-1.5 ng/ml predicts a slight postoperative period, when the value of the level of ghrelin >1,5-10,0 ng/ml predicted for moderate, with an increase of ghrelin levels in serum >of 10.0 ng/ml predict a severe course of the postoperative period.
FIELD: veterinary medicine.
SUBSTANCE: method includes the interaction of antigens with antibodies, with anti-species antibodies labeled with horseradish peroxidase, adding the substrate mixture and recording the results of the reaction on the colour intensity of the complex formed. In the reaction the plates are used with antigens of rotavirus, coronavirus, the virus of diarrhea preliminary sorbed on them, and after applying the test samples of serum 0.10-0.12 ml each. The plates were incubated for 1.5-2 h at 36-37°C and washed. Then total anti-species immunosorbent conjugate is applied of 0.10-0.12 ml consisting of enzyme-labeled antibodies against globulins of blood serum of cattle and the results of the reaction are recorded.
EFFECT: invention provides simultaneous diagnosis of rotavirus, coronavirus enteritis, viral diarrhea in cattle, sensitivity, specificity, and ease of the assay, and the ability to automate the process of research.
SUBSTANCE: for the purpose of prediction of developing atopic dermatitis in newborns, umbilical blood plasma is examined for a level of interleukin-18. If the related level is 34 pg/ml or lower, developing atopic dermatitis in the newborn is predicted.
EFFECT: use of the given method enables well-timed evaluation of a risk of developing atopic dermatitis in newborns and early preventive measures for prevention thereof.
SUBSTANCE: method under the invention provides that the complex immunoglobulin preparation containing a component of C3b complement is sorbed in microplate wells for the purpose of immunoassay. Then the microplate wells are added with a solution of an analysed sample containing human complement C1 inhibitor with the unknown activity. It is followed by incubation, drying and washing of the dish; the wells are added with a conjugate of enzyme with serine proteinase in the form of preparation of fibrinolysin and a substrate of this enzyme. The amount of the prepared enzymatic reaction product is used to derive the content of active C1 inhibitor. A kit for implementing the method comprises a flat-bottomed microplate with bound C3b, the conjugate of enzyme with serine proteinase, the substrate buffer of this enzyme and a reference for active C1 inhibitor.
EFFECT: use of the method under the invention enables determining activity of C1 inhibitor, simultaneously bound both with serine proteinase inhibition, and with binding inhibition of complement factor B and C3b complement component.
2 cl, 1 tbl, 1 dwg
SUBSTANCE: method under the invention provides that the preparation pyrogenal is sorbed in microplate wells for the purpose of immunoassay; then the microplate wells are added with a solution of an analysed samples containing complement human C1 complement inhibitor with the unknown concentration. Then the plates are incubated, and after washing and drying, a conjugate of enzyme with C1 inhibitor antibodies and a substratum of this enzyme are added into the wells. The amount of the prepared enzymatic reaction product enables calculating the content of the C1 inhibitor in the analysed sample. A kit for implementing the method comprises a flat-bottomed microplate with the sorbed preparation of pyrogenal, the conjugate of the enzyme with the human C1 inhibitor antibodies, a substrate buffer of this enzyme and a reference for the C1 inhibitor.
EFFECT: use of the method enables implementing quantitation of the C1 inhibitor using an ability of the latter to bind to lipopolysaccharide.
2 cl, 1 dwg, 2 ex
SUBSTANCE: method under the invention provides that the preparation heparin is sorbed in microplate wells for the purpose of immunoassay. Then the microplate wells are added with a solution of an analysed samples containing complement C1 inhibitor with the unknown concentration. It is followed by incubation, and after washing and drying of the dish, a conjugate of enzyme with C1 inhibitor antibodies and a substratum of this enzyme are added into the wells. The quantitation is enabled by the amount of the prepared enzymatic reaction product. A kit for implementing the method comprises a flat-bottomed microplate with the sorbed preparation heparin, the conjugate of the enzyme with the human C1 inhibitor antibodies, a substrate buffer of this enzyme and a reference for the C1 inhibitor.
EFFECT: use of an ability of the C1 inhibitor to bind to heparin to determine its content in the analysed samples.
2 cl, 2 dwg, 2 ex
SUBSTANCE: pharmaceutical preparation CIP (complex immunoglobulin preparation, consisting of IgG, IgA and IgM) is bound in cavities of a micropanel, said preparation containing a C3b complement component, samples containing the determined functionally active human C1 inhibitor are incubated in the cavities, and the bound C1 inhibitor is determined using a conjugate of an antibody against the human C1 inhibitor with an enzyme and a substrate for that enzyme based on the amount of the formed product of the enzymatic reaction. The set contains flat-bottomed micropanel with the sorbed CIP, the enzyme conjugate with antibodies against the human C1 inhibitor, a substrate buffer of that enzyme and a standard for the active C1 inhibitor.
EFFECT: use of the method enables to determine activity of the C1 inhibitor which regulates the complement alternative path.
2 cl, 1 dwg, 1 tbl, 2 ex
SUBSTANCE: complex antigen of measles virus used as a component of immunoenzymometric test system for diagnostics of antibodies to measles virus was produced from culture liquid containing measles virus stain Leningrad 16 with titer no less than 105.0 50% tissue cytopathic dose/ml, Edmonston - no less than 106.0 50% tissue cytopathic dose/ml, NovO/96 no less than 108.0 50% tissue cytopathic dose/ml by inactivation of its infectious activity by detergent and separation of protein from cellular lysate by chromatographic purification in amount no less than 2 mcg/ml with the purity no less than 70%. Culture virus-containing liquid was processed using separate cultivation of measles virus stains Leningrad 16, Edmonston and NovO/96 on monolayer of Vero cells culture with subsequent mixing of culture virus containing liquid in proportion 1:1:1 v/v.
EFFECT: Usage of invention provides for increased sensitivity and specificity of a complex antigen possessing the property to detect antibodies in blood serum.
2 cl, 4 tbl, 6 ex
FIELD: chemistry; biochemistry.
SUBSTANCE: invention concerns medical virology and microbiology. Strain is deposited in culture collection of Federal State Research Institution State Research Centre of Virology and Microbiology "Vektor" of Rospotrebnadzor, under registration No VB-05. Strain features higher productivity. More sensitive immunoenzyme test system for hepatitis virus antibody diagnostics is created on the basis of this strain. Invention can be applied in virology.
EFFECT: production of more sensitive immunoenzyme test system for hepatitis virus antibody diagnostics.
2 cl, 1 dwg, 1 tbl, 4 ex
FIELD: medicine; ophthalmology.
SUBSTANCE: invention is intended for simultaneous verification of uveal melanoma and forecast of metastasis development. Immunohistochemical analysis of protein S-100 and melanin-A expression within tumour cell is accompanied with simultaneous estimation of S-100-positive cells number within visual field. Uveal melanoma is verified if reaction of S-100 and melanin-A is positive with forecasted high possibility of tumour dissemination in case number of S-100-positive cells number is less than 50.
EFFECT: simplified examination associated with high specificity and sensibility of simultaneously verified uveal melanoma and forecasted metastasis.
1 tbl, 1 dwg, 2 ex
FIELD: medicine, veterinary.
SUBSTANCE: specific antibodies to virus of goose enteritis are determined by IEA where virus received following injection of epizootic strain "P-75" of enteritis virus, is used as an antigen, and macro porous glass with diameter not less than 700 is used for purification of virus-containing material, the goose Ig G specific immunoperoxidase conjugate is used as an anti-specific agent, and the IEA results are calculated by use of the formula: titer = antiIg[2.02(lg S/P)+3.51], where S is for optical density of tested serum; P is for optical density of the positive control.
EFFECT: method reduces the test time and economical costs.
2 tbl, 2 ex
FIELD: medicine, ophthalmology.
SUBSTANCE: in lacrimal liquid one should detect the content of interleukin 8 (IL-8) and that of interleukin 1 beta (IL-1β) to calculate prognostic coefficient (PC) due to dividing the first value by the second one by the following formula: At PC value being below 10.0 one should predict favorable disease flow, and at PC value being above 10.0 - unfavorable flow.
EFFECT: higher accuracy of prediction.
FIELD: medicine, ophthalmology.
SUBSTANCE: one should detect the content of tumor necrosis factor alpha in acute period, moreover, the above-mentioned factor should be determined in lacrimal liquid on the 11th - 15th d against the onset of herpetic ophthalmoinspection, at its content ranged 176-250 pcg/ml prediction is considered to be favorable, and from the value of 300 pcg/ml and higher - as unfavorable.
EFFECT: higher accuracy of prediction.
FIELD: medicine, surgery.
SUBSTANCE: one should carry out virological testing patient's blood serum and hepatic bioptates. At detecting TTVDNA and HGVRNA it is necessary to perform ultrasound survey, and at availability of biliary sludge one should conclude upon early stage of cholelithiasis.
EFFECT: higher accuracy of diagnostics.
FIELD: medicine, cardiology.
SUBSTANCE: one should detect the level of activity of IgM and IgG immunoglobulins to cytomegalovirus on the 5th and 15th d of large-focus myocardial infarction. At increased diagnostically valuable result of specific immunoglobulins of type M by 0.10-0.20 times and for type G by 0.73-2.09 times it is possible to predict favorable clinical flow of large-focus myocardial infarction without exudative pericarditis. At the value of specific immunoglobulins exceeding diagnostically valuable result for type M - by 0.86-1.67 times and for type G by 2.42-3.01 times one should predict early clinical flow of post-infarction pericarditis. The method enables to carry out prophylactic measures in due time.
EFFECT: higher accuracy of prediction.
5 ex, 2 tbl
SUBSTANCE: one should test blood serumal samples in immunoenzymatic assay by applying a test system ELI-N-1, the components of which are being antigens of nervous tissue or their immunochemical analogs specifically binding antibodies with tendency to antigens of nervous tissue, and, also, idiotypical antibodies to them, or their variable parts. Prediction should be performed according to the level of idiotypical and anti-idiotypical autoantibodies to antigens of nervous tissue and, also, according to their ratio. Thus, a new test system has been elaborated that enables to predict the flow of available nervous-psychic diseases.
EFFECT: higher accuracy of prediction.
2 cl, 7 ex
FIELD: medicine, obstetrics.
SUBSTANCE: in pregnant women at pregnancy terms being after 39 wk in average portion of morning urine one should detect due to a solid-phase immunoenzymatic assay the concentration of pregnancy-associated protein-A (PAPP-A). At the level of PAPP-A being 768.2 ng/ml and more it is possible to conclude upon physiological mature pregnancy. The innovation is noninvasive and enables to increase accuracy in predicting true over-mature pregnancy.
EFFECT: higher efficiency and accuracy of diagnostics.
2 ex, 1 tbl
FIELD: medicine, biotechnology.
SUBSTANCE: the present innovation deals with elaborating diagnostic reagents for testing prionic protein in mammalian cerebral tissue due to IEA technique and refers to antibodies specifically reacting with prionic protein PrP or its fragment. The innovation includes polyclonal antibodies obtained to synthetic peptides including amino acid sequences of bovine prionic protein 143-168, 101-134 and 211-241, their conjugates with horseradish peroxidase and diagnostic reagents obtained upon their basis that enable to detect prionic protein in mammalian cerebral tissues.
EFFECT: higher specificity of detection.
15 cl, 8 ex, 8 tbl
FIELD: veterinary and medicine.
SUBSTANCE: invention, in particular, relates to production and use of biological preparations intended for differential diagnostics of brucellosis and to a method of differentially diagnosing brucellosis. Method involves serologic analysis of sera using antigenic "IFK", which is horse-radish peroxidase-labeled electrophoretically purified polypeptide fraction of virulent strain B.arborus 54. Diagnosis of brucellosis is stated when anti-brucellosis antibodies in sick animal sera diluted to 1/100 and higher are revealed at CSP reaction intensity 2.1 and higher.
EFFECT: elaborated method increasing immuno-enzymatic test specificity and allowing performing differentiation of postvaccinal immunological reactions and postinfectious reactions induced by microorganisms having antigenic affinity with brucellas, especially Yersinia enteriocolitica.
2 cl, 4 ex
FIELD: biotechnology, analytical chemistry.
SUBSTANCE: claimed method includes providing of complexes between antigen molecules and specific antibodies on carrier surface, wherein said complexes are disclosed by addition of enzyme label thereto followed detection thereof based on formation of enzyme reaction product. Enzyme label addition is carried out by two protein interaction, namely bacterial ribonucleaze barnase and barstare, which is inhibitor thereof, wherein either of the two is added to immunoreagent, and the other one is added to enzyme label. Abovementioned complexes have high affinity, specificity, and binding constant of 1014 M-1.
EFFECT: new method for antigen detection.
6 dwg, 2 ex
FIELD: veterinary virology.
SUBSTANCE: the present innovation deals with interaction of antibodies with an antigen, with antibodies labeled with horseradish peroxidase, addition of substrate mixture and registration of reaction results. Moreover, one should apply plotting boards with presorbed antibodies and general immunoenzymatic conjugate. The innovation enables to shorten terms for diagnosis and obtain more significant results of diagnostics.
EFFECT: higher efficiency.