Method of propagation by herbaceous cuttings of japanese camelia (camellia japonica l)

FIELD: agriculture.

SUBSTANCE: invention relates to the field of agriculture. In the method the cuttings of Japanese camelia of annual growth consisting of five healthy buds and whole leaves 5-8 cm long are propagated. Then the cuttings are treated with an aqueous solution of physiologically active substances containing in one liter of working solution of 10 mg indolyl-3-butyric acid, 1 mg kinetic, 0.5 mg gibberellin A3, 5 mg of ascorbic acid and thiamine chloride. Exposure of treatment is 1 hour, with humidity of 70% -80%, temperature +22°-25°, and the technological sequence of propagation by herbaceous cuttings enables to receive up to 60% of the yield of vegetatively propagated own-root plants.

EFFECT: method enables to propagate, produce, store and obtain genetically homogeneous high quality plants.

4 tbl

 

The invention relates to agriculture and is intended for the production of micro-propagated own-rooted plants Camellia Japanese. The method includes the procurement of green cuttings, processing their solutions of physiologically active substances, rooting. The formation of adventitious roots is through the use of minimized low concentrations of physiologically active substances that allow it to grow (produce) own-rooted plants Camellia Japanese. Thus created the opportunity to ensure the production of cheap, genetically uniform, high-quality material, duplicate forms and samples in sufficient quantity for their subsequent implementation into production.

Known in Japanese Camellia methods of propagation: seeds, cuttings, root suckers, grafts, cuttings.

The essence of these methods of reproduction are as follows:

Reproduction by seeds: Fertilization and maturation of the seeds of Camellia Japanese occurs at different times of the year, with the duration of development from 210-220 days. Fruit - capsule. Fruiting, and flowering plants of seed origin, begins at 5-8 year of vegetation. The box in the process of vegetation from green becomes brown in color, indicating at the beginning of the ripening seeds, tinkerable crack, the shell of the seed is painted in dark brown color, and cotyledons fills the entire cavity of the shell. The full maturation of the seeds in a box is not at the same time. Therefore, since the cracking unit, slightly brown boxes, you need to start harvesting. In view of the content in the seed of a large amount of oil, they lose their germination especially when stored in unsuitable for seed temperature conditions. By the end of the third month of storage the seeds of Camellia Japanese completely lose germination. In this regard, seeding should be done after gathering at the stage of incomplete aging and drying of the outer shell. The optimum sowing date and seed Camellia Japanese on the black sea coast of the Caucasus is the period from September to October.

To speed up the germination and emergence of amicable shoots before planting they should be treated in warm water for 12-24 hours at room temperature. In the process of soaking the appropriate two or three times to change the water. To the substrate for seeding high demands: they should have good air permeability, high moisture, acidic with a pH of 4.5-6.0, and must contain a sufficient number of batteries for the next good development of seedlings. These include: soil mixture that includes two parts brown the forest soil and one part peat, or a substrate consisting of peat, perlite and sand, in a volume ratio of 2:1:1.

Because the substrate is filled plastics receptacles intended for sowing seeds, racks or rows in the greenhouse, it condense on the surface of the substrate make shallow 4-5 cm lateral grooves with a distance of 10-15 cm Seeds are sown 2-3 cm from each other by a depth of 3-4 cm Surface sown areas covered with substrate thickness of 1.5 cm and lightly compacted by a special Board. Pour water, using a fine spray net. When sowing seeds in containers, to maintain humidity and heat preservation of their covered with glass. To reduce fluctuations in temperature, the glass surface is covered with paper. When sowing seeds on shelves or rows, they should be close arcs with a polyethylene film of a thickness of 130 to 150 microns.

In the spring of next year, when the average daily air temperature is +18°-22°, seeds begin together to sprout. When two true leaves is pick seedlings. When the seedlings are seated in pots with a volume of 450 ml plastic containers with a volume of 450-550 ml, or on beds. For fun use one of the above-described loose rich in organic matter substrate. For the best development of the seedlings need partial shading. Good growth and development of the e seedlings ensures high cultivation techniques. Seedlings grown with closed root system in pots, plastic bags, easy to processes of budding and grafting, planting permanent root completely. Grown seedlings are given by way of reproduction in the second year of life, with the root collar thickness of 3 mm or more suitable for use under inoculation, vaccination, and for other purposes.

The disadvantages of generative mode of reproduction Camellia Japanese are:

- complexity (26,5%) receiving high-quality planting material;

- material costs, leading to high cost of planting material;

- long from 5-8 years duration of the period of entry of seedlings flowering;

- inability to obtain uniform planting material due to heterozygosity.

The reproduction layers: the Camellia, like many other plants can be propagated by layering. This method of reproduction is the oldest used in ornamental horticulture. The essence of the method of reproduction is that the stimulation of the formation of adventitious roots on the escape is achieved until it separates from the mother plant. The method is simple because it does not require special conditions for the maintenance of various environment settings rooting for the viability of escape in the process rhizogenesis activity of tissues. D. the I method of breeding using scheme trench planting the original planted, which allows early next spring to bend to the ground annual branch with the lower tier plants, put them in the prepared groove depth of 15-25 cm, fasten with pins or hooks, and then covered with soil so that the tip of the shoot remained outside. On the branches in the ground before pinning removed the leaves from the stalks. The lower curve makes an oblique incision escape and inserted into an incision match to expand it. This will accelerate kalosorisate, and hence the rooting. To stimulate rooting of shoots can be used, okolicsanyi stem copper wire. You can use okolicsanyi knife bark of the stem with a width of 6-8 mm and 50% it can be deleted.

The soil covering layers, should be light, nutritious, slightly acidic pH environment, moderately moist, thus favouring the development of the root system of layers. After discharge of the shoots of the previous year vegetation rooting occurs within three to four months, so the process of reproduction layers should begin before the onset of the period favorable for planting young plants.

The essence of the formation of adventitious roots is that by mechanical interference disrupted the traditional flow of nutrients and hormones in the stalk of Godech is on the increase. The determining factor in rooting cuttings is soil moisture, which needs constant monitoring, especially during dry periods. Rooting cuttings usually occurs within one growing season of mother plants. In autumn it is separated from the mother plant, to the newly formed plant has adapted to the conditions of their existence in the world around him. Own-rooted nucleus can be transplanted into a pot, container or to a new location in the soil. If the root system by the autumn underdeveloped, it does not dig until next year.

The lack of reproduction layers is that in this way it is impossible to obtain a sufficient quantity of planting material. From the same plant, depending on the condition and the availability of suitable for layers of branches, receive from 1-3 pieces, and there are plants, which are not formed shoots suitable for cuttings. On the grounds set out the method of vegetative propagation of Camellia Japanese layering is uneconomical, but in practice can be applied in the private sector ornamental horticulture.

Reproduction root offspring: the Term "offspring" is the lateral escape, aboveground or underground, formed from buds in the root collar area. Emerging offspring of the Japanese Camellia rooted for a long time, slowly. In order to speed up their reproduction, in the spring they should be separated from the mother plant and planted in the substrate, which is either in pots, special plastic containers or on shelves. The substrate must have good air permeability, high water-holding capacity (72-75%) and acidic.

The grafted plants, if the coppice shoots formed, it is removed as formed from the rootstock. If escape was formed on own-rooted plant, then, of course, may be of interest for subsequent reproduction. Monitor the growth and development of vegetative plants aged 5-30 years showed low activity formation both above ground and underground offspring. Thirty adults of flowering plants, of unknown nature, origin, formed 14 offspring in length from 9.0-12,8 see cost-based on rooting and the above features, a method of vegetative propagation of Camellia Japanese offspring is time-consuming, costly, protracted period of rooting, and therefore is not of practical interest to obtain a sufficient quantity of planting material.

Propagation by grafting: Grafting is called the connection of the two parts of plants, so that they grow together and continue to develop it is to a single organism. One component vaccinations is called the Scion, is part of the stem of the plant which is to be reproduced. It grafted on the root system of another plant is called a seedling stock. As a rootstock for Camellia Japanese use seedlings aged 1-2 years and more, preferably with closed root system, root collar thickness from 3-6 mm. Seedlings with the development parameters is grown by the above described method of generative reproduction. Propagation by grafting creates the opportunity for one plant to combine one or more grafts. This is particularly useful when planting individual plants, with different tsvetogamma, on small plots of land. To achieve good results when using different methods of vegetative propagation is important that a practical work were taken into account properties of polarity, as this determines the success of the results of vaccination. It is also important to properly connect the various tissues of the Scion and rootstock that they have grown together as quickly as possible. Under the bark of the stem is actively growing layer kambalny tissues (cells), so it is important to position the fabric of rootstock and Scion so that these tissues are in contact or had more contact. Method of propagation by grafting, in relation to the Japanese Camellia, describes the propagation of the varietal, butt and into the split.

As a result, set out the sequence of preparation of the grafts should be timed to coincide with the period of spring SAP flow rootstocks, i.e. to the beginning of the vaccination. The stock is cut at an angle of 35°-45° at a height of 4-5 cm from the surface of the substrate and the top of it make a bevel cut with a knife the length of 2-3 cm Makes an oblique slice and at the base of the Scion length of 2-3 cm, at the same angle as that of the Scion. That is, sections of the Scion should be made at the same angle as the rootstock. D is I the strength of the connection on the rootstock and the Scion make the tabs. Cut the rootstock, departing at one third from the top end, make a shallow longitudinal incision of 1.5-2.0 see the same incision made from Scion, departing on the third from the end of the oblique slice. Scion connected with seedling stock so that the tongue of one gone beyond the tongue of another. If the stem of the rootstock thicker than that of the Scion, the last shift to one side to ensure good contact cambium layers. The connection of the Scion and rootstock tied PVC film so that the coils of the garter overlaps the top and bottom slices. The tip of the Scion grease garden pitch, put a label.

Spring is warm, with adequate supply of moisture grafted plants, promotes good healing of tissues. Once on the surface of the slice is formed callus, and this means, began the accretion of parts vaccinations, banding made from PVC film shoot. If below accrete vaccination starts growing offspring, it is removed. When using this method of vegetative propagation, obtained a good survival rate Kopeliovich seedlings Camellia.

Grafting in cleft is an affordable method of vegetative propagation of ornamental crops. For its implementation in early spring to prepare rootstocks and grafts by the above mentioned methods of generative and vegetative propagation.

Vaccinations usually wire the t in the root of the neck or in the small trunk of the rootstock (hypocotyl). With a sharp knife cut horizontally stock. In the middle of a cut do vertical cut (split) depth of 2.5-3.0 see Desirable Scion to pick up the same diameter as stock, because it promotes better healing of tissues. Above the top Bud of the Scion make a bevel cut. From the lower end of the cutting form the wedge two oblique sections with a length of 2.0-2.5 cm: one oblique slice start near the kidneys, the other on the opposite side. The Scion with a little effort put into the split on the rootstock. Part of the slice remains outside. This kind of "window" will accelerate the accretion of the wound surfaces of the callus. The junction of the stock and Scion tightly tied PVC film. The tip of the Scion cover garden pitch, in order to reduce evaporation. As intergrowths of Scion, the film is cut and removed. Shoots formed below the fused inoculation, remove.

The lack of propagation by grafting and cleft is the inability to obtain sufficient quantities of planting material. The process is time-consuming with high material costs, which lead to a significant increase in the cost of planting material. However, find application in the selection process and in the private sector ornamental horticulture.

Propagation by cuttings. Reproduction Camellia green is by cuttings is a promising method of vegetative propagation. And allows from one breeding plants to get a large number of new-rooted plants with valuable agronomic traits, allows to preserve the purity of varieties and available production. Important conditions for rooting cuttings of Camellia are the period of their establishment and the selection of the Scion. Cuttings summer (July-August) period are optimal for rooting in paleodrainage condition, the annual growth this year. It handles with well-developed 3-4 vegetative buds provide quick and good rooting. However, in the presence of the shoot, with five or more vegetative buds, it is possible to prepare two cuttings from the upper and middle part. It handles with well-developed vegetative buds rooted 50-80 day, have a strong increment and earlier bloom.

Approved and what to ceramcoat the Camellia is possible from July to September and from December to February, this is done using paleodrainage cuttings of the current year increase. We use mainly the apical cuttings with 3-4 buds and perpetuate them in greenhouses on heated shelves when the air temperature is around +20°, soil to +30°. Research on green cuttings of Camellia Japanese in terms of Adjara showed that cuttings for rooting can be prepared at any time the year when the shoots are not in a state of growth. The best time to consider rooting periods before spring growth of shoots (February-March) and after the spring growth (June-July), when the shoots are completely not woody. The authors close their recommendations about periods, terms and condition selected for cuttings, one in that the substrates for rooting of green cuttings of Camellia should be slightly acidic, containing large amounts of organic matter, good air permeability. Single and in relation to environmental factors rooting: humidity of 70%-80%, temperature from +18°-30°, the largest used cuttings for rooting. Believe that the cuttings with 3-4 well developed buds, with a length of about 5-8 cm are the best value for their intended use.

The authors group the measures taken to improve the described method of propagation of Camellia Japanese green cuttings. It is established that the treatment of cuttings with 2% solution of IAA within 7 hours contributes to the development of a strong root system, unlike raw. It is shown that the rooting of cuttings can be improved by treating them indol-3-butyric acid at a concentration of 20 mg/l within 24 hours. In addition, cuts cambial tissues near the base of the cuttings before they are processed by the stimulator on ViDi the WMD improves rooting. Pre-treatment with an aqueous solution of indol-3-butyric acid at a concentration of 20 mg/l increases the rooting of cuttings of Camellia Japanese. Analysis of the results of works on improvement of green grafting shows that as the physiologically active component is most often found indol-3-butyric acid at a concentration of 20 mg/L.

Despite the positive influence of physiologically active substances on rooting of green cuttings of Camellia Japanese can't say about the election varietal reaction cambial tissues. The absence to date of effective ways of propagating camellias, based on modern achievements of biological Sciences, inhibits proliferation entries valuable genetic offspring, and especially the manufacture of homogeneous high-quality planting material for further meet the growing needs of our population in planting material of its own production.

When this constrained the development of ways of reproduction, contributing to a significant expansion of the distribution area of culture and agricultural practices for a possible substantial increase in commercial production.

In the absence of other developed methods of vegetative time is norene, the method of propagation of green cuttings of Japanese Camellia (green cuttings) chosen as a prototype. However, the prototype has disadvantages, namely, that certain provisions of green grafting in our experimental studies have not been confirmed. Have not confirmed the effectiveness of various physiologically active substances, their concentration in the processes of rooting of green cuttings of Camellia Japanese.

The aim of the invention is to develop a method green grafting camellias, Japanese, involving the principles of biological stimulation cuttings of different concentrations and ratio of physiologically active substances auxinfo and cytokinin origin, contributing to the dynamic growth and development of adventitious roots.

This object is achieved in that for vegetative propagation of Camellia Japanese green cuttings use cuttings from five healthy leaves of two varieties Elegans and Goffredo Odero. Varieties differ greatly, is poorly studied, characterized by low average to 25.6%, rooting. These differences with regard to the optimal concentration of physiologically active substances allowed to develop the way green grafting camellias of Japan. Rasoherina activity of tissues of green cuttings of Camellia Japanese examined according to the scheme experience (p is iLounge 1 - see the end of the description).

In option # 1 cuttings were treated with 2% solution of IAA with exposure time of seven hours. In option # 2 cuttings were treated in an aqueous solution of indol-3-Macalloy acid exposure 24 hours. In embodiments St, St1, 3, 4, 5 cuttings were treated in aqueous solution with an exposure of one hour.

In research on rhizogenesis activity tissue cuttings of perennial crops proved a positive effect of ascorbic acid (vitamin C) and thiamine chloride. With these works, our research into appropriate ways of experience were included in the 5 mg/l of each component. In addition, in the processes of cultivation of tissues in vitro, proven highly effective sharing of physiologically active substances: kinetin, indolyl-3-butyric acid and gibberellin in the regenerating activity of the tissues. In this context it was of interest to test them in various concentrations and ratios in the process of green grafting camellias. For this reason, in the scheme experience were included low concentrations of these reagents.

That is, due to the expected impact of vitamins, kinetin and gibberellin in the scheme experience included the second version of the control. In variants from 20-22 green cuttings of Camellia Japanese. On each stalk five healthy kidneys and entire sheet. Procurement cherenkovanija in the morning, in the summer, the first week of June. Cut the cuttings annual increment sort, tie into bundles for ease of handling in solutions of physiologically active substances. Stimulation cuttings performed according to the scheme experience, including seven options, including two controls. To do this well in advance to prepare the uterine solutions of the individual components of the environment. All cooked uterine solutions are contained in glass containers and stored at +2°-4° temperature, within 30 days (Appendix 2). From the mother solution is prepared working solutions based on 1.0 liter of distilled water. The solutions are poured into glasses with a wide throat and immerse them in the cuttings to stimulate to root formation. After the exposure processing of cuttings, they are taken out of the solution and cover with a damp cloth.

As a substrate for rooting of green cuttings of Camellia Japanese used perlite fraction No. 2, having humidified it first with water to 75% humidity. Perlite filled volume of 0.4 m3PVC containers. A smooth flat plate straighten the surface of perlite, then wooden transona condense so that the layer thickness was 12-14 see the Cuttings are planted in perlite under the scheme 5×5×10 cm planting depth of 1.5-2.0 cm, giving them a slight tilt. Humidity environment rooting is maintained at 70%-80%, the dispersion is ion spray of water. The temperature of rooting is within +22°-25°. The technological sequence, taking into account the parameters and modes of rooting, has a positive effect on the growth and formation of adventitious roots of green cuttings of Camellia Japanese.

In numerous studies with evergreen cultures there is no consensus about the role of obliterati in rhizogenesis activity of tissue grafts. Although this fact is of great practical interest. In this regard, the us was founded on experiment to study the relationship between the area of the leaf blade with rhizogenesis activity of tissues of green cuttings of Camellia Japanese varieties Elegans (Annex 3). Experimental evidence suggests that the increase in the number of leaves on the cuttings leads to an increase in the area of assimilation apparatus. The increase in leaf area is positive, based on the percentage of rooted cuttings. It is established that the cuttings with five leaves area 118,6 cm2have a high regenerative activity, rooting period of 55 days and provide 60% yield rooted cuttings. The increase of the leaves on the stalk from 5 pieces and above leads to the death of the Scion. This fact, associated with the use of green cuttings with five leaves, formed the basis of the EC the pilot studies for the development of the method of green grafting camellias of Japan. Study of the characteristics of root formation, taking into account various concentrations and ratios of physiologically active substances, showed a positive impact on rhizogenes activity adventitious roots of green cuttings of Camellia Japanese greatest influence of physiologically active substances observed in option six, containing in one liter of an aqueous solution of 10 mg indol-3-butyric acid, 1 mg of kinetin, 0.5 mg of gibberellins And3, 5 mg of ascorbic acid, 5 mg of thiamine chloride. This ratio of physiologically active substances, in comparison with other variants of experiences contributes to 60% of the output of micro-propagated plants, rooting period from 55-60 days (Appendix 4, 5). In addition, studies have shown that the positive effect of physiologically active substances on different varieties manifests in different ways and amounts to 5% of the total number of rooted cuttings of Camellia Japanese.

Comparative analysis of the claimed technical solution with the prototype shows that the proposed method of vegetative propagation of Camellia Japanese green cuttings (green cuttings) differs completely, but above all, ease of execution, processing mode (stimulation) of green cuttings of physiologically active substances, low minimized the concentration of the mi physiologically active substances, the method of procurement of green cuttings.

Similar features of the declared technical solution vegetative method of propagation of green cuttings of Camellia Japanese in comparison with the prototype, we have not identified during the study of this and related areas, which ensures compliance with the criterion of "inventive step".

The proposed method of vegetative propagation of Camellia Japanese green cuttings is industrially acceptable, allows to copy, reproduce, store and retrieve genetically homogeneous plants with minimal cost. The way green grafting Japanese Camellia includes reproduction cuttings annual increment, consisting of five healthy kidney and five leaf length 5-8 cm, followed by processing them with an aqueous solution of physiologically active substances containing in one liter of working solution of 10 mg indol-3-butyric acid, 1 mg of kinetin, 0.5 mg of gibberellins And35 mg of ascorbic acid and thiamine chloride, with an exposure processing 1 hour, humidity 70%-80%, +22°-25°, allow up to 60% of the output of micro-propagated own-rooted plants. Technological sequence using the above techniques green grafting Japanese Camellia helps to reduce material and labor costs and production the military area.

Sources of information

1. Hartmann HT, Kester, DOUGLAS Reproduction of garden plants. Moscow, 1963, s-438.

2. Jincharadze NM Camellia on the black sea coast of Adjara. Publishing house "Sabchota Adjara, Batumi, 1974, 100 S.

3. Ovcharov CE Vitamins plants. Moscow, 1969, s-260.

4. Prikhodko, S. Instructions for vegetative propagation of Camellia Japanese by grafting. Kiev, USSR Academy of Sciences. Botanical garden. 1959, 8 S.

5. Tarasenko M.L. Green garden cuttings and forest crops. Moscow, Izd-vo ICCA, 1991, s-247.

Appendix 1

The SCHEME EXPERIENCE

1. St control (distad)

2. St1control - "C" - 5 mg/l + Thiamine · CL - 5 mg/l + kinetin 1 mg/l + gibberellins And30.5 mg/L.

3. I - IAA 2% solution

4. II - Indol-3-butyric acid 20 mg/L.

5. III - Indol-3-butyric acid 15 mg/l + "With" 5 mg/l + Thiamine · CL - 5 mg/l + kinetin 1 mg/l + gibberellins And30.5 mg/L.

6. IV - Indol-3-butyric acid 10 mg/l + "With" 5 mg/l + Thiamine · CL - 5 mg/l + kinetin 1 mg/l + gibberellins And30.5 mg/L.

7. V - Indol-3-butyric acid 5 mg/l + "With" 5 mg/l + Thiamine · CL - 5 mg/l + kinetin 1 mg/l + gibberellins And30.5 mg/L.

Seven options in the scheme experience.

Annex 2

Uterine and working solutions of the drugs used for processing green black is s Camellia Japanese, 2010.
No.The components of the mother solutionsConcentration of the mother solutions*The amount of stock solution to prepare 1 liter of working solution**
1Ascorbic acid (vitamin C), 0.5 mg/mlIn 100 ml bidistilled water dissolve 50 mg drug10 ml
2Thiamine · CL - 5mg/mlIn 100 ml bidistilled water dissolve 50 mg drug10 ml
3Gibberellins And30.5 mg/mlIn 50 ml of bidistilled water dissolve 25 mg drug1 ml
4IAA, 2 g/100 mlIn 100 ml bidistilled water dissolve 2.0 grams of the drug100 ml
5Indolyl-3-butyric acid, 2 mg/mlIn 0.5 ml of 0.1 N KOH was dissolved 100 mg of the drug and the amount of dissolved drug was adjusted to 50 ml10 ml; 7.5 ml; 5.0 ml; 2.5 ml
6Kinetin, 1 mg/mlTwo drops of 0.01 N KOH was dissolved 50 mg of the drug and the amount of dissolved drug was adjusted to 50 ml1 ml
Note: * uterine solutions contain in glass containers with cut and stored at +2°-4° temperature.
** Selection of the required number of drug to make 1 liter of working solution is made by a glass pipette, syringe or automatic dispenser.

Annex 3

20
The impact of obliterati cuttings of Camellia Japanese varieties Elegans their rooting, 2010.
№№ p/pNumber of cuttings options (units)Number of leaves on the cuttings (pieces)The area of leaves on the stalk (cm2)The period of the rooting of cuttings (days)Number of rooted cuttings (pieces)%% the number of planted cuttingsThe number of transplanted grafts in the rearing (pieces)
1243,6907357
220353,1709456
3204a 94.26011553
4205118,65012602
5206141,70009
Note: Processing of cuttings physiologically active substances produced according to the fourth variant of the scheme experience.

Annex 4

Features of rooting of green cuttings of Camellia Japanese varieties Coffredo Odero, 2010.
No.For different versionsThe number of planted cuttings (pieces)The number of rooted cuttings (pieces)%% the number of planted cuttingsThe number of transplanted grafts in the rearing (pieces)
1St*203150
2St1**202100
3I22627,20
4II22522,70
5III228 36,35
6IV2210to 45.42
7V20735,05
Note: * St - control - processing of green cuttings for 1 hour in distilled water.
** St1control processing of green cuttings in a solution containing 5 mg/l ascorbic acid + 5 mg/l thiamine-chloride + 1 mg/l of kinetin + 0.5 mg/l gibberellin And3.

Annex 5

Features of rooting of green cuttings of Camellia Japanese varieties Elegans, 2010.
No.For different versionsNumber of planted cuttings (pieces)Number of rooted cuttings (pieces)%% count planted cuttingsThe number of transplanted grafts in the rearing (pieces)The number of CEREC is formed in generative buds (pieces)
1St*2021060
2St1**2031570
3I2042060
4II2063070
5III2094537
6IV20115565
7V 84063
Note: * St - control - processing of green cuttings for 1 hour in distilled water.
** St1control processing of green cuttings in a solution containing 5 mg/l ascorbic acid + 5 mg/l thiamine-chloride + 1 mg/l of kinetin + 0.5 mg/l gibberellin And3.

The way green grafting Japanese Camellia - Camellia japonica L. includes reproduction cuttings annual increment, consisting of five healthy kidneys and entire leaf length 5-8 cm, followed by processing them with an aqueous solution of physiologically active substances containing in one liter of working solution of 10 mg indol-3-butyric acid, 1 mg of kinetin, 0.5 mg of gibberellins And35 mg of ascorbic acid and thiamine chloride, with an exposure processing 1 h, humidity of 70-80%, the temperature of 22-25°C, With the sequence of technological methods of green grafting allows you to get up to 60% of the output of micro-propagated own-rooted plants.



 

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6 cl, 7 dwg

FIELD: agriculture.

SUBSTANCE: light diode radiator comprises a body from a heat conductive material, at least partially ribbed at the rear side. The body has an outlet hole, which is closed with an optically transparent protective glass or a diffuser. Inside the body there are linear boards installed with assembled groups of light diodes with a different spectrum of radiation in the range of spectral efficiency of photosynthesis /400-700 nm/ with optical axes, facing the outlet hole of the body, and connected to a source of supply. At least on two internal side walls of the concave body there is a cascade of longitudinal plates forming terraces from a heat conductive material, which create ribs of an internal radiator of a conductive heat sink. Longitudinal plates are in thermal contact with body walls and face the outlet hole with a flat part. On each plate there are linear boards /lines/ installed in thermal contact, mostly boards with an aluminium base with high-capacity light diodes or light diode modules, or separate light diodes, which are connected in series or in parallel-serial chains to a source of supply.

EFFECT: design will make it possible to improve thermal and spectral characteristics, to increase density of radiation flow with reduced dimensions of a radiator.

6 cl, 7 dwg

FIELD: agriculture.

SUBSTANCE: invention relates to agriculture and may be used to grow vegetable crops. The method to form vertically oriented vegetable crops, including installation of vertical supports in the form of plastic pipes filled with a cement mortar and fixation of a guide wire on them. At the same time, prior to installation of vertical supports, at their opposite ends, through transverse holes are made with a diameter that is not less than the diameter of the guide wire. Then they are filled with a cement mortar. After their installation the wire is arranged in holes of serially arranged supports; for its fixation with the cement mortar. Between vertical supports at the lower and upper level of the wire with even pitch spiral guides are fixed, inside which a stem or several stems of a vegetable crop are screwed in.

EFFECT: method makes it possible to facilitate growing of vegetable crops.

1 dwg

FIELD: agriculture.

SUBSTANCE: invention relates to agriculture and can be used to control the climate in the greenhouse. The system comprises a controller unit, a control unit, subsystem of measurement sensors, and actuating mechanisms. The subsystem of measurement sensors comprises sensors of parameters of air and soil in the greenhouse and the sensors of environmental parameters. The actuating mechanisms (AM) represent the fanlight opener, a fan, a drive of the screen, a governor gear of carbon dioxide supply, and nodes of heating circuit. The outputs of the control unit are connected to the AM with the ability to manage them according to the values of the parameters measured by the sensors.

EFFECT: system of climate control provides increased efficiency of optimisation of quality of regulation.

5 cl, 1 dwg

FIELD: agriculture.

SUBSTANCE: invention relates to a method of plant cultivation. The method includes filling the open latticed shell, made of durable waterproof fibers, with filler comprising sawdust mixed with perlite and plant seeds. After filling the shell is bound, and the closed latticed shell is obtained with filler in the form of a blank, having the properties of flexibility and plastic deformation. Further, it is deformed from all its sides and by crumpling it and sculpting three-dimensional shape of moulding; it is attached from the outside with cotton threads, which are located on its perimetre to give rigidity to the blank, allowing to keep the obtained shape. Then the blank is dried in a drying chamber for 40-60 hours at the temperature of 40-50°C and air humidity of 40-60% to give it a potential rigidity and dryness, eliminating crushing of the blank during its transportation to the place of plant growth. Then the obtained blank is periodically dipped in water or a nutrient solution based on water, kept in water until sawdust is fully saturated with water, expose to sunlight and kept in the light. The above operations are carried out until germination through the cells of the latticed shell of the plant sprouts and their appearance on the outer surface of the shell, and then they are stopped dipping into the water, and the water is supplied on the latticed shell by its irrigation.

EFFECT: increased efficiency of plant growth and ensuring environmental cleanliness in its cultivation.

3 cl, 19 dwg

FIELD: agriculture.

SUBSTANCE: invention relates to the field of agriculture. The method includes application of sewage sludge into the soil and processing the plants with biostimulant, as which the organosilicone immunostimulant adaptogen Mival-Agro is used at a concentration of 0.05%. Peeled and notched gladiolus bulbil with a diameter of 4-7 mm is placed in a solution of Mival Agro and is kept in a warm place up to 12 hours, after which the imbibed bulbil is powdered with ash of buckwheat husk and planted in the soil. In addition, ash of buckwheat husk of 100 g/m2 is applied into the soil, and sewage sludge is applied at a dose of 12 kg/m2.

EFFECT: method enables to improve the commercial properties and to obtain healthy planting material of gladiolus.

2 tbl, 3 ex

FIELD: agriculture.

SUBSTANCE: device of automatic control of mist-generating plant relates to gardening, namely to vegetative propagation of horticultural crops by the method of herbaceous cuttings. The device comprises operating mode switches on the number of units of mist-generating plant, a commutation switch to connect the power source to the units of mist-generating plant and cyclical timing relay that determines the duration of the presence or absence of each unit power. The cyclical timing relay consists of a microcontroller, a real-time clock, a memory module, two encoders, control buttons and an alphanumeric LCD display.

EFFECT: device of automatic control of mist-generating plant provides optimisation of watering mode by an independent set of time of watering and the time of pause separately for several intervals within the day, such as morning, day, evening and night.

1 dwg

FIELD: agriculture.

SUBSTANCE: invention relates to the field of agriculture. The method lies in treatment of seeds and plants of spring wheat in the tillering stage and the booting stage with the agent which is made of spring wheat grain cultivar "MIS" from the nursery-garden of the first year of propagation by five-day infusion of 10 g of crushed seeds in 10 ml of 96% ethanol. After that 10 ml of 96% ethanol is added to the infusion and is infused for another 14 days, after which the infusion is poured, squeezed and the sediment is filtered from which the product is prepared in a centesimal dilutions based on distilled water. The treatment is carried out at the temperature of 19-24°C at a distance of 30-40 cm in the second centesimal dilution of the agent for seeds of spring wheat and at the third centesimal dilution of the agent for plants of spring wheat in the tillering stage and the booting stage.

EFFECT: method enables to improve the biological activity of grain and plants in various stages of development.

2 cl, 2 tbl

FIELD: chemistry.

SUBSTANCE: invention relates to the technology of producing compositions for mulching different types of soil, lawn and parking areas. The aqueous mulching composition contains lignosulphonate, carbamide, NA-carboxymethyl cellulose, potassium chloride and as natural additives - Andreaeopsida moss and common pine needles, in different amounts which are reflected in versions of the polymer-natural mulching compositions.

EFFECT: use of the composition improves the environment.

9 cl, 1 tbl, 12 ex

FIELD: chemistry.

SUBSTANCE: invention relates to the technology of producing compositions for mulching different types of soil, lawn and parking areas. The aqueous mulching composition contains lignosulphonate, carbamide, NA-carboxymethyl cellulose, potassium chloride and as natural additives - Moerckia moss and Pinus strobes pine needles, in different amounts which are reflected in versions of the polymer-natural mulching compositions.

EFFECT: use of said compositions improves the environment.

9 cl, 1 tbl, 12 ex

FIELD: chemistry.

SUBSTANCE: invention relates to the technology of producing compositions for mulching different types of soil, lawn and parking areas. The aqueous mulching composition contains lignosulphonate, carbamide, NA-carboxymethyl cellulose, potassium chloride and as natural additives - Bryopsida moss and Juniperus virginiana 'Hibernica' juniper needles, in different amounts which are reflected in versions of the polymer-natural mulching compositions.

EFFECT: use of said compositions improves the environment.

3 cl, 1 tbl, 3 ex

FIELD: chemistry.

SUBSTANCE: invention relates to the technology of producing compositions for mulching different types of soil, lawn and parking areas. The aqueous mulching composition contains lignosulphonate, carbamide, NA-carboxymethyl cellulose, potassium chloride and as natural additives - Tetraphidopsida moss and Pinus pumila pine needles, in different amounts which are reflected in versions of the polymer-natural mulching compositions.

EFFECT: use of said compositions improves the environment.

9 cl, 1 tbl, 12 ex

FIELD: agriculture.

SUBSTANCE: aqueous composition of mulch cover contains lignosulphonate, carbamide, NA-carboxymethyl cellulose, potassium chloride; and moss of species Takakiopsida and needles of juniper Juniperus virginiana "Erecta" as natural supplements in different amounts reflected in the composition versions of the polymer-natural mulch cover.

EFFECT: use of these compositions improves ecology of environment.

3 cl, 1 tbl, 3 ex

FIELD: agriculture.

SUBSTANCE: invention relates to the field of physiology and plant breeding. In the method the seeds are sown in capacities, which are metal boxes, plastic trays and containers. They are frozen in freezers with automatic control of temperature regime, and the extent of frost-resistance is evaluated. In this case, at formation of the third leaf - in the tillering phase the plants are placed in a cooling chamber - showcase for northwarding and maintained at a temperature of 0 to +3°C for 2 days. Then they are transferred to freezers and left for 2 days at a temperature of -5°C. Then the temperature is lowered by 1°C every hour to the critical -12°C-13°C and thawed in the reverse order of 1°C every hour to 0°C. Then two days later the freezers are switched off, the capacities with plants are unloaded and on the third day after unloading the condition of the plants are determined visually according to a 9-point scale, and the frost-resistance in points: 1 - very low, 5 - average, 9 - very high.

EFFECT: method enables to reduce the period of evaluation, to simplify and improve the accuracy of determining the degree of frost-resistance.

4 dwg, 1 ex

FIELD: agriculture.

SUBSTANCE: invention relates to agriculture, in particular, to technologies and automated control devices of technological processes of agricultural products production in crop husbandry. The device comprises a first unit of artificial vision, placed on the mobile apparatus and the video signal processing unit. The device comprises an unmanned aerial vehicle, the second unit of artificial vision, two units of data transmit-receive, testing and control unit, unit of orientation in three-dimensional coordinates. The unmanned aerial vehicle has the ability to move within the cultivated land. The first unit of data transmit-receive, the unit of built-in testing and control, the unit of orientation in three-dimensional coordinates are mounted on the ground mobile apparatus. The second unit of artificial vision and the second unit of data transmit-receive are located on the unmanned aerial vehicle. The output of the second unit of artificial vision is connected to the input of the second unit of data transmit-receive. The output of the first unit of data transmit-receive is connected to the first input of the unit of built-in testing and control. The output of the unit of orientation in three-dimensional coordinates is connected to the second input of the unit of built-in testing and control.

EFFECT: invention provides enhanced functionality of production regulation of agricultural products.

3 dwg

FIELD: agriculture.

SUBSTANCE: invention relates to the field of agriculture and forestry. In the method the containers are placed on the 15-centimetre drainage base in greenhouses. The mixture is prepared which consists of three parts: thin middle-humous clay loam leached chernozem, organic matter and sand. The river sand as the substrate is applied to the surface, the green cuttings are prepared and planted to a depth of 2.5 cm. Multiple atomised spraying with water is carried out, as well as three-times watering with the preparations: 1% working suspension "Planriz" with a titer 2×109 cells in 1 ml and 0.001% working fluid "Rostok" of a 1% solution of humic acids at the interval of 15 days and twice watering with 0.5% working suspension of the preparation "Trichodermin" with a titer 1-2×107 conidium in 1 ml at the interval of one month. At the same time, the containers of size from 5×5 to 6×6 cm or the diameter of 5 to 6 cm, and the volume of 90-110 ml are filled with the mixture, the layer of river sand is 1-2 cm. The containers are placed on the drainage base in greenhouses, planting scheme of green cuttings of softwood is 5.5×5.5 cm, the rate of working fluid and working suspensions is 1 litre/1 m2.

EFFECT: method enables to reduce the area for rooting green cuttings, rate of working fluid and suspensions of preparations, and to increase rooting of green cuttings of softwood.

2 tbl

FIELD: agriculture.

SUBSTANCE: in the method the electromagnetic light flux is supplied from the emitter. In this case, the light flux or its part is polarised, mixed with non-polarised, if any, and reflect in the direction of plants, for example, during the growing season of plants. The incident light flux is directed in part or in full in the area of Brewster angle. In the light flux or its part the polarisation density is periodically changed from the minimum, for example, zero, to maximum. The period of the density changing of polarised effluxion is established depending, for example, on the type of plant. When the intensity of the reflected light is not higher than the threshold, the refractive index in it of the reflector film is changed smoothly or discretely, for example, within the selected extended area of Brewster angle. The incident light flux is previously diffused with, for example, the same reflector or the light source.

EFFECT: method enables to improve the vegetation and viability of plants, to reduce the area of sowing seeds.

6 cl, 2 dwg

FIELD: agriculture.

SUBSTANCE: invention relates to the field of agriculture and can be used in cultivation of potatoes on seedbeds. The method includes application of solid organic and mineral fertilisers, local basic processing of tuber area, potato planting, pre-emergence surface treatment of a seedbed, removal the drip irrigation system and harvesting. The local basic processing of tuber area is carried out with the width not exceeding 70 cm, using front-type plows or rotary cultivators. The seedbed is formed with organic-soil zone and feeding elements of drip irrigation systems near potato tubers. The seedbed is created by a single exposure of screw-type plow bodies.

EFFECT: method enables to improve efficiency of potato cultivation in conditions of insufficient humidifying.

1 dwg

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