Method of treating necrotic ulcers of oral mucosa in patients with multiple myeloma considering free radical oxidation in oral fluid
SUBSTANCE: invention refers to medicine and aims at treating necrotic ulcers of oral mucosa in patients with multiple myeloma. Luminal-dependent chemiluminescence of oral fluid is conducted to determine fluorescence light sum and maximum flash. If the light sum value is within the range of 1.02 to 6.6 standard units, and the maximum flash value is 0.7 to 2.5 standard units, the toothpaste Lacalut fluor is used 2 times a day and solcoseryl dental adhesive paste 4 times a day after meals and before bedtime to relieve symptoms completely. The light sum value 8.3 to 13.5 standard units and the maximum flash value 3.4 to 4.2 standard units, the toothpaste Colgate total propolis is used 2 times a day and the film preparation Oblecol three times a day to relieve symptoms completely.
EFFECT: use of the invention reduces a length of epithelisation of necrotic ulcers of oral mucosa.
The invention relates to medicine, namely to the dentist, and is intended for treating ulcerative-necrotic lesions of the oral mucosa (SOR) in patients with multiple myeloma (MM) in the context of chemotherapy.
There is currently no clear idea about how the treatment and prevention of ulcer-necrotic lesions in oncohematological patients, due to their atipichnoi manifestations in terms of specific gravity nosology and cytostatic therapy. According to medical records and interviews of patients with MM average period of epithelialization of the ulcer-necrotic lesions SOR is 15-20 days. Manifestations of ulcerative-necrotic lesions SOR in patients with MM in terms of chemotherapy leads to the necessity of searching for optimal methods of treatment taking into account the pathogenetic mechanisms of development of this disease. The study of free radical mechanisms in the pathogenesis of ulcerative-necrotic elements contributes to the development of highly efficient methods of correction of free radical oxidation (CPO) in this disease.
Reliably role violations of SRO in the pathogenesis of many dental diseases that causes the application in their treatment of drugs that affect the formation of free radicals. Carrying out correction of a free radical status of the oral cavity is mainly associated with the treatment of inflammatory diseases of the periodontium. However, the study of mechanisms of development of ulcerative-necrotic lesions SOR MM patients indicate a significant role in their breaches occur volatile stage of phagocytosis, such as the imbalance of oxidant-antioxidant balance.
In some clinical work established that the degree of violation of the oxidant-antioxidant equilibrium depends on the stage of disease, tumor size (Kirsi GV, Belenkov O.Y, Sharipov F.K., Juraev PPM Dependence of lipid peroxidation in cancer of the stomach from the size of the tumor // Clinical laboratory diagnostics, 2004; Aliasova AV, Kontorshikova PHD, Bach BA, 2006), and the activity of chemotherapeutic programs (Bogush T.A. Reduce the toxicity of anticancer drugs is a way to increase the effectiveness of treatment of malignant tumors / Taugus // Matters. Oncology. 1995. No. 2. - P.52-53). Violations antioxidant balance, in turn, enhance the cytotoxic effect of chemotherapeutic agents against healthy tissue weighting thus adverse effects of therapy.
In recent years, more and more researchers are realizing the necessity of antioxidants together with chemotherapy to improve treatment efficacy and reduce side effects of cytostatics (Welser SL Application medicinalplants "deplin" with Galavit and lincomycin for the local treatment of stomatitis, induced by chemotherapy of cancer: Dis. Kida. the honey. Sciences. Moscow, 2005. - 101 C.). The most studied application of antioxidants such as tocopherol acetate, ascorbic acid, selenium, β-carotene, vitamin a and aevit, qudesan (Prasad K.N. Multiple dietary antioxidants enhance the efficacy of standard and experimental cancer therapies and decrease their toxicity / K.N.Prasad // Integr. Cancer Ther. - 2004. - Vol.3, No. 4. - P.310-322; Petrova this YEAR Features the development of chronic inflammation in the oral cavity in lymphoproliferative diseases: Dis. Prof. the honey. Sciences. Novosibirsk, 2009. - 266 S.).
There is a method of treating ulcerative-necrotic lesions of the mucous membranes of the oral cavity, including anesthesia, antiseptic and keratolytic treatment, anti-inflammatory and regenerative effect, with anti-inflammatory and regenerative effect is carried out in one stage by application with suspension of alloparental 3-5 times a day for 5-10 minutes, the course of 3-4 days, followed by the application of stabilizing drug (application EN 2004134873 published 10.05.2006,).
The prototype of the invention is a method for correcting free radical oxidation in the oral fluid in inflammatory periodontal diseases, namely, that in the oral fluid, record levels of chemiluminescence, the maximum flash from 0.8 to 5 conventional units is setosum from 1.5 to 25 conventional units, assessed as low activity of free radical oxidation, and in the complex treatment administered prooxidants, maximum flash from 6,55 to 25 and setosum from 26 to 45 conventional units, estimated as high activity of free radical oxidation in the complex treatment administered antioxidants (patent RU 2140633, 1999).
The objective of the invention is to develop an effective method of treating ulcerative-necrotic lesions of the oral mucosa in patients with multiple myeloma.
The technical result - the reduction of the period of epithelialization of the ulcer-necrotic elements of the oral mucosa in 1,5-2 times due to adequate treatment taking into account the activity of oxidative processes in the oral fluid.
The proposed method is as follows. The chemiluminescence (CHL) oral fluid is performed on the device Chemiluminometer-003 (Interuniversity laboratory technical systems biomedical research Bashkir state medical and Ufa state aviation technical University, the Branch register "Medical industry of Russia and CIS countries").
The collection of saliva spend the morning on an empty stomach by Slavyane in a sterile tube for 10 minutes.
In the measuring cuvette add 2.0 ml of working solution lyuminola (0.5 ml stock solution l is minola DMSO in 500 ml of physiological solution) and 0.1 ml of oral fluid (RJ). The cuvette is placed in chemiluminometer and thermostatic at 37°C. Induced luminescence was measured for 5 minutes. The process of measuring PI and processing of the results is carried out in automatic mode with the help of special programs.
Study the following indicators: S - sutasoma glow - square from the contour to the curve chemiluminescence; Andmax- the maximum amplitude of the glow from the contour to the highest point of the curve. All the data is expressed in arbitrary relative units relative to the standard glow SFHM-1, amounting to 5.1·105quantum/sec.
Patients with low values of indicators chemiluminescence: S from 1,02 to 6.6 conventional units, Andmaxfrom 0.7 to 2.5 conventional units for correction of SRO in the mouth use of prophylactic toothpaste, stimulating the generation of radicals reactive oxygen species - Lacalut fluor and solcoseryl dental adhesive paste (SDAP), which, along with keratoplasticheskie action has a prooxidant effect. SDAP applied by a standard method on the area of lesions 4 times a day after meals and before bedtime, to complete epithelialization of the ulcer-necrotic elements.
Patients with elevated values of chemiluminescence: S from 8.3 to 13.5 conventional units, Andmaxfrom 3.4 to 4.2 conventional units use toothpaste with antioxidant AK is ewnetu - Colgate total propolis and film preparation "Oblaka" (based on sea buckthorn oil), stimulating reparative processes, accelerating the healing process and with antioxidant and cytoprotective effect. The film "Oblaka" cut out the required size and immersed in 0.9% saline. Before applying the film to the affected surface is pre-cleaned of fibrinous film chymotrypsin by a standard method. On top of the film put a gauze pad. The film "Oblaka" impose 3 times a day until complete epithelialization of the ulcer-necrotic elements.
In the available scientific and medical and patent literature information about the popularity identical correction method SRO in the oral liquid in treating ulcerative-necrotic lesions SOR in patients with MM is not detected. Thus, the claimed invention meets the criterion of "novelty".
The authors proved that the application of the method shortens the period of epithelialization of the ulcer-necrotic lesions in the mouth due to the correction of the SRO in the oral fluid. Thus, the claimed invention meets the criterion of "inventive step".
The proposed method treated 24 patients with multiple myeloma. In all cases, was achieved this technical result.
Example 1. Patient K., 52 years old. Di is the prognosis Multiple myeloma, Stage III. Diffuse osteoporosis flat bones. Plasmacytoma of the neck. Chemotherapeutic stomatitis.
Complaints about pain when eating, talking.
When inspecting the element lesion was located on the lateral surface of the tongue size 13 mm Ulcer was rounded with fuzzy uneven edges, with a clean surface, sharply painful to palpation, with no signs of inflammation.
The patient in the morning on an empty stomach was spat the saliva into a sterile tube. In the measuring cuvette was added 2.0 ml of working solution lyuminola (0.5 ml stock solution lyuminola DMSO in 500 ml of physiological solution) and 0.1 ml of RJ. The cuvette was placed in chemiluminometer-003. Findings: sutasoma - 2,4 conventional units, the maximum flash - 0,9 conventional units, suggesting a low activity of free radical oxidation. In the complex treatment of traditional dental activities the patient was scheduled preventive Lacalut toothpaste fluor and solcoseryl dental adhesive paste. Toothpaste was used 2 times a day, morning and evening. A strip of paste SDP length of about 0.5 cm was applied in a thin layer on pre-dried gauze swab the affected area of oral mucosa and slightly moistened caused pasta water. The procedure was repeated 4 times a day after meals and before bedtime. The treatment was carried out before the disappearance the Oia symptoms.
On the same day, there was a decrease in pain, on the 2nd day was marked by the appearance of granulations. Ulcer epithelials on the 6th day. Performance chemiluminescence on 6th day: sutasoma - 5,3 conventional units, the maximum flash - 1,4 conventional units.
Example 2. Patient M., aged 57. Diagnosis: Multiple myeloma, stage III. Myeloma destruction of the bones. Nephropathy. Chemotherapeutic stomatitis.
Complaints about pain when eating and talking, the impossibility of wearing complete dentures.
When viewed on the mucosa of the right cheek on the line joining the teeth and the mucous membrane of the hard palate was determined sores round shape with clear smooth edges, covered with fibrinous film, redness and swelling at the periphery. Ulcer dimensions of 1.0 and 1.4, see the Patient in the morning on an empty stomach collected saliva into a sterile tube. In the measuring cuvette was added 2.0 ml of working solution lyuminola (0.5 ml stock solution lyuminola DMSO in 500 ml of physiological solution) and 0.1 ml of RJ. The cuvette was placed in chemiluminometer-003. Findings: sutasoma - 12,6 conventional units, the maximum flash - 4,1 conventional units, testified to the high activity of free radical oxidation. In the complex treatment of traditional dental patient events were assigned toothpaste Colgate total ropolis and film preparation "Oblaka". Toothpaste was used 2 times a day, morning and evening. The film "Oblaka" cut out the required size and immersed in 0.9% saline. Before applying the film, the skin surface was pre-purified from fibrinous film chymotrypsin by a standard method. On top of the film put a gauze pad. The film "Oblaka" were applied 3 times a day until complete epithelialization of the ulcer-necrotic elements.
Complete relief of pain came on 2nd day. On the 2nd day there was a decrease in congestion. Complete epithelialization of the ulcer was achieved on the 7th day. Performance chemiluminescence on the 7th day was: sutasoma - 8,9 conventional units, the maximum flash - 3,5 conventional units.
The application of the proposed method of treating ulcerative-necrotic lesions SOR patients with MM with regard to free-radical oxidation in the oral fluid is adequate pathogenetic treatment to reduce the period of epithelialization of the ulcer-necrotic elements.
The proposed method is easily reproducible and can be used in dental clinics and in the hospital. Thus, the claimed invention meets the criterion of "industrial applicability".
A method of treating ulcerative-necrotic lesions of the mucosa from patients is now with multiple myeloma with regard to free-radical oxidation (CPO) in oral fluid, including Lomonosovskiy chemiluminescence oral fluid, determining the maximum flash and sutasoma glow, holding a Pro-oxidant therapy in low activity SRO or antioxidant therapy in increased activity of SRO, characterized in that when the values of sutasoma from 1,02 to 6.6 units, and the maximum flash from 0.7 to 2.5 conventional units as a Pro-oxidant therapy using Lacalut toothpaste fluor and callserially adhesive paste when the values setosum from 8.3 to 13.5 units, and the maximum flash from 3.4 to 4.2 conventional units as antioxidants use toothpaste Colgate total propolis and film preparation "Oblaka", moreover, the treatment until epithelialization of the ulcer-necrotic elements.
FIELD: instrument making.
SUBSTANCE: device to detect and identify chemical compounds includes chamber of analysed chemical compound entry, chamber of ions analyser, analyser of ions connected to device of data processing. Also device comprises membrane permeable for analysed compounds and arranged between specified chambers, system for supply of sample comprising analysed compound, source of ionisation. Besides, device includes facilities of connection to systems of provision of working atmospheres of chambers, sources of supply and sources of electric working fields. Besides, membrane is arranged with possibility to form ions of analysed chemical compounds on its surface as it is exposed to electromagnet radiation or flow of particles, and source of ionisation is set with the possibility to send electromagnet radiation or flow of particles directly at surface of membrane. At the same time source of ionisation is arranged in the form of source of electromagnet radiation optically joined via scanning device with surface of membrane.
EFFECT: increased sensitivity of method to detect organic compounds, simplified process to produce ions of analysed chemical compounds, reduced requirements to parametres of laser and expanded field of method application and device to detect and identify chemical compounds.
29 cl, 5 dwg
SUBSTANCE: invention relates to atomic emission spectral analysis. The method includes sample introduction into aflame of plasma, registration of the sequence of atomic emission spectrum during the flow of the sample into the plasma, calculation of the intensity of the analytical spectral line of the desired element depending on the arrival time of the sample into the flame of the plasma, determining by the calibration dependence of concentration of the desired element in the sample, and determining the distribution of particles of the sample by mass. The concentration of all the desired elements is determined simultaneously due to the fact that each spectrum sequence is recorded in the spectral range, including the analytical spectral line of the desired elements. Calculation of the intensity of the analytical spectral lines in carried out in each spectrum taking into account the background under the line, after this the dependence concentration of the desired element is obtained from the arrival time of the sample into the plasma, and as per the indicated dependencies the mass of the particles and the concentration of the desired elements in the particles are determined.
EFFECT: providing simultaneous determination of the distribution of particles by mass in a dispersed sample.
6 cl, 4 dwg, 2 tbl
FIELD: engines; testing methods.
SUBSTANCE: invention relates to methods of determining parameters of simple wear particles consisting of one element and complex wear particles consisting of several elements found in oil system of engine for determining type of developing defect. According to proposed method, parameters of all wear particles found in oil system of engine are considered at one to failure. In sample of lubricating oil taken from rear drive box, amount of metal wear particles is determined and parameter of these particles, i.e. elementary content, and mean size of particles of each element in oil sample, concentration of dissolved metal and metal in wear particles are found. Simultaneously with taking oil sample, wash-out sample from main oil filter is taken and parameters of wear particles in this sample are measured and reference values of ratings of wear particles are calculated in form of ratio of simple and complex particles in wash-out sample, amount of particles of each element and amount of complex particles of definite composition in total amount of particles washed from oil filter, rating of wear particles of definite element and rating of complex particles are determined. Basing of relationship of values of parameters of wear particles in samples of oil and oil filter wash-out samples type of developing defect in engine oil system is determined.
EFFECT: improved information content when determining type of developing defect in engine oil filter.
FIELD: spectral analysis.
SUBSTANCE: method uses one-electrode high-frequency plasma charge in mode of alternating pulses. In accordance to direction of gas along, perpendicularly or oppositely to plasma-generating electrode different constructions of device burners are used. Recording of radiation spectrums is performed in direction dependent on type of used spectrometer: perpendicular to said generated charge for slit spectrometer and in parallel for diaphragm spectrometer.
EFFECT: higher sensitivity, lower costs.
2 cl, 4 dwg
FIELD: medicine, pharmaceutics.
SUBSTANCE: what is presented is using water-soluble hybrid macromolecular compounds O-(3-(3,5-di-tert-butyl-4-hydroxyphenyl)propionyl-(1→6)-α-D-glucan and polyethylene glycol bis-3-(3,5-di-tert-butyl-4-hydroxyphenyl)-propionate as erythroprotective agents for phenylhydrazine poisoning. It is shown that the course intravenous introduction of the compounds has manifested erythroprotective action.
EFFECT: invention extends the range of agents applicable in integrated therapy of phenylhydrazine poisoning.
1 tbl, 3 ex
FIELD: medicine, pharmaceutics.
SUBSTANCE: invention refers to pharmaceutical industry, namely a method for preparing an antioxidant homogenous sterile holothurian solution. The method for preparing the antioxidant homogenous sterile holothurian solution consisting in the fact that raw residual tissue is washed, ground, placed in containers and exposed to heat in a steam steriliser at pressure by formula min./degree wherein n is optimal tissue degradation time 55 to 65 min.
EFFECT: method provides preparing the homogenous sterile solution showing high pharmacological value.
SUBSTANCE: invention refers to medicine, particularly, the use of human lactoferrin apo-form as an antihypoxant and a hypoxia inducible factor-1 alpha stabiliser.
EFFECT: invention provides the use of the natural iron chelator lactoferrin, no toxicity, hypoallergenicity, an ability to penetrate through the bowel into the blood flow and through the blood-brain barrier.
2 tbl, 1 dwg
FIELD: medicine, pharmaceutics.
SUBSTANCE: invention refers to pharmaceutical and cosmetic industry and concerns preparing shelf fungus extract. A method for preparing shelf fungus extract involving extraction of ground shelf fungus in sodium or potassium hydroxide under certain conditions; the prepared extract is filtered.
EFFECT: shelf fungus extract prepared by the method described above shows high antioxidant activity and prolonged shelf life.
1 tbl, 2 ex
FIELD: medicine, pharmaceutics.
SUBSTANCE: invention refers to pharmacology and medicine and concerns the use of dihydrobromide 9-(2-diethylaminoethyl)-2-(3,4-dioxyphenyl)imidazo[1,2-a]benzimidazole of formula as a biologically active compound possessing high antihypoxic, actoprotective, nootropic activities and having a positive effect on physical efficiency, and a based pharmaceutical composition.
EFFECT: preparing the compound possessing high antihypoxic, actoprotective, nootropic activities and having a positive effect on physical efficiency, and the based pharmaceutical composition.
2 cl, 8 tbl, 3 ex
SUBSTANCE: method involves the introduction of lead acetate and a calcium-containing substance. For this purpose, 10% calcium chloride 0.3 ml per 200 g is introduced in rats once a day daily through a probe into a stomach for 20 days. In 4 days from the beginning of the experiment, it is combined with the subcutaneous introduction of lead acetate 40 mg/kg at a metal basis for 16 days.
EFFECT: method is easily reproducible, cost-saving and moreover provides relieving toxic action of lead effectively.
5 tbl, 2 dwg, 1 ex
FIELD: medicine, pharmaceutics.
SUBSTANCE: invention refers to medicine, namely an agent exhibiting antioxidant, photoprotective and geroprotective action, and may be used for development of pharmacological preparations neutralising an adverse health effect of UV light and visible ranges, and inactivating toxic action of the age pigment. The agent represents an interaction product of the equimolar amounts of 6-hydroxy-2-aminobenzothiazole and N-acetyl-L-cysteine on boiling in an ethanol solution.
EFFECT: invention provides high antioxidant activity and high stability under visible and UV light.
SUBSTANCE: group of inventions refers to medicine and aims at preventing and treating a liver diseases. What is used is xanthohumol of formula (I) or xanthohumol metabolite as an active substance for making a preparation for preventing and/or controlling the liver diseases caused by obesity and/or diabetes. The daily dose varies within 0.01 to 161 mg/kg of weight. The xanthohumol content in the composition makes 0.1 to 99 wt %.
EFFECT: group of inventions allows having an effect on viral replication and cancer cell apoptosis, delaying developing cirrhosis.
25 cl, 10 dwg, 2 ex
SUBSTANCE: present invention relates to use of compounds of formula Ia, Ib or Ic or cosmetically acceptable salts thereof as antioxidants, for preparing cosmetic compositions and for controlling pigmentation, particularly for clearing areas on the skin, to certain specific compounds of formula Ia, Ib or Ic or cosmetically acceptable salts thereof, a method of producing compounds of formula Ia, Ib or Ic, a cosmetic composition based on compounds of formula Ia, Ib or Ic and preparation method thereof or . In formulae la, lb or Ic, each of R2-R6 and R9-R13 is independently selected from H, OH, straight or branched C1-C20-alkoxy groups, straight or branched C1-C20-alkyl groups.
EFFECT: obtaining compounds used as antioxidants.
14 cl, 9 ex, 3 tbl, 1 dwg
SUBSTANCE: invention refers to medicine. The presented therapeutic agent in the form of a pill contains oxymethyluracyl and mebeverine hydrochloride as active substances, as core-coating excipients - mixed potato starch and lactic sugar in relation 1:1; mixed 5% alcohol-acetone acetyl phthalylcellulose (APC), 10% aqueous potato starch and 0.1% aqueous styrene maleic anhydride copolymer (SMAC) in relation 1:1:1 as a humidifier 1; mixed 3% aqueous Kollidon 90F, povidone and 0.1% aqueous SMAC in relation 1:1 as a humidifier 2; 5% APC as a humidifier 3; mixed 64% sugar syrup and 1% Kollidon 25 in relation 5:1 as a humidifier 4 in certain proportions.
EFFECT: invention provides preparing the new therapeutic agent possessing anti-inflammatory, recycling, antispasmodic and antioxidant actions, with uniform distribution and complete enteric solubility in 2 hours, with retard action during 1 hour.
4 dwg, 2 tbl, 5 ex
SUBSTANCE: invention relates to medicine, namely to psychotherapy, reflexotherapy, physiotherapy. Method includes carrying out bioresonance testing (BRT) of functional state by means of hardware-software complex Vitatest. After that, with patient in lying on back position, transcranial electrostimulation (TES) and trance guidance at the background of aromatherapy and relaxation music for 20-25 minutes. After that, painful zones on hands and feet are detected and reflexotherapeutic impact is performed on detected zones until pain disappears. Further impact is performed depending on detected during BRT disorders. In case of dominating asthenic-depressive manifestations impact is performed in accordance with Su Jok correspondence system on points and zones of brain projection of anti-depressive direction, located on pads of distal feet phalanxes, reflexotherapy of corporal points VC14 jujue 1.5 cun lower than xiphoid precess of sternum, on the middle line of abdomen, anti-depressive herbal drugs with are administered for one month from beginning of cycles of procedures. In case of asthenic and vegetative-vascular manifestations rexlexotherapy is performed on points of heart projections on palms and little toes, on corporal points C4, C9 with administration of stimulating medications - bioadaptogens for 1-2 months. In case of anxiety and phobic disorders, R1 points are stimulated with intake of sedative phytopreparations for 1-3 months. In case of chronic fatigue impact is performed on corporal points TR1, point and deep massage of cervical-collar zone is performed and course of neuroprotectors - essential amino acids is administered for 1 to 3 months symptoms disappear completely. In case of post-trauma stress disorder, reflexotherapeutic impact is performed on the 1-st, 6-th and 11-th days in corporal points IG-8, J-17by sedative method, on the 2-nd, 7-th, 14-th day in points F-8, J-4, R-12 - by tonic method with intake of allopathic and homeopathic preparation with sedative action for 1-3 months.
EFFECT: method reduces treatment terms with increase of remission duration from 10 months to one year.