Method of estimating concentration of resin-like substances in suspension

FIELD: chemistry.

SUBSTANCE: method involves titration of a suspension with a solvent which is soluble in water and dissolves the suspended resin-like substance until reducing light scattering, which is determined either visually or using optical devices which enable to measure the amount of light passing through the liquid.

EFFECT: rapid estimation of the amount of resin-like substance suspended in water.

3 dwg


The invention is intended for use in experimental and industrial biotechnology, in particular to assess the concentration of retinoid substances in suspension, intended, including for reducing microbial contamination of feed and food, as well as air aerosols obtained by spraying suspensions. The objective is achieved by titration of aqueous suspensions of one of the polar solvents retinoid substances, such as ethyl, methyl, isopropyl alcohol, acetone, and others, to dissolve the suspended particles, which is fixed on the transfer medium from turbid to transparent state (to reduce light scattering due to the dissolution of the light-scattering particles). The transition record, either visually, without the use of measuring tools, or by using optical devices (turbidity meters, colorimeters), which measures the amount of light transmitted through the suspension (solution).

Suspension natural retinoid compounds in recent times are increasingly used to reduce bacterial contamination food and feed fat emulsions, work surfaces and indoor air industries, as well as in the areas of medical, veterinary and agricultural purposes, in the office and residential premises[1, 2, 3].

Receive the Ute suspension retinoid substances, extracted from natural products [4], using, for example, ultrasound [5], and in all cases, the concentration of suspended substances is determined very roughly, that depends on a widely varying properties of the original product (for example, resin, pine [5], propolis [6] and others). No doubt, however, that the measurement of the concentration of retinoid compounds in suspension is essential for dispensing their effects on the microflora and the human and animal organisms.

There are many different ways of measuring the concentration of suspended substances [7].

Nephelometry, for example, based on the ability of colloidal systems diffuse light, allows to determine the concentration of the colloidal system and the average size of the colloidal particles. The effect of the turbidity meter is based on comparing the intensity of the light scattered investigated Sol, with the intensity of light scattered by a standard Sol. About the content of the substance in the test sample is judged either by the intensity of light scattering, determined by the number of light-scattering particles values (analysis method), or by weakening of the light flux formed by the suspension (turbidimetric method of analysis). Methods nephelometry allow only a rough estimate of a substance in suspension, as recorded l is nil number of particles, but not their sizes.

Some methods use relative measurements, as a rule, for the automatic control of production processes and do not allow to determine the absolute content of suspended matter [8, 9]. There are ways restricted, for example, for particles with pronounced magnetic properties [10], is obviously not suitable for measurement of retinoid suspensions of substances.

There are also methods titrimetric analysis based on the measurement of the amount of reagent needed to interact with the designated component in the solution in accordance with the stoichiometry of chemical reactions between them. The greatest distribution was received by titrimetry for rapid determination of high and medium concentrations of substances in solutions [11].

Each of the above methods are unsuitable for measuring the concentration of retinoid substances in suspension, contains the element, the combination of which allows you to determine the amount of suspended substances.

The present invention is directed to the implementation of the rapid assessment of the amount of suspended retinoid substance using simple laboratory equipment.

We stated the result is greater efficiency analysis by conducting a rapid assessment of the quantity of suspended platforms the aqueous resin like substance in the water is achieved by titration, for example ethyl alcohol or acetone aqueous suspension retinoid substance, to its transformation into a clear solution. Since the effect is reversible, then the method can be calibrated by titration, for example, alcohol solutions of water, before the formation of the suspension by lowering the solubility of the resin in the water-alcohol solution.

An example implementation of the invention

Aqueous suspension of resin larch titrated with ethyl alcohol until the disappearance of turbidity, shaking the sample after adding the next portion of the solvent, 1, or alcohol solution is titrated with water, shaking the sample after adding the next portion of water until turbidity, Fig.2. The concentration of the resin is determined by the calibration curve, Figure 3, is constructed taking into account the reversibility of the effect, i.e. adding to the alcohol solution of the resin in water until the turbidity of the solution. The turbidity of the solution is determined visually with or without the use of optical instruments for determining the turbidity of the liquid medium.

The calibration method is possible by using the reverse effect, i.e. the comparison of the results obtained by the claimed method, with the results of the titration of water-alcohol solutions retinoid substances with known concentration.

Thus, the set of distinctive features of the described method provides DOS is iunie the specified result.

As a result of the analysis of the level of technology assessment of the retinoid content of substances in an ultrasonic water suspensions source, characterized by signs, identical with all the essential features of the claimed invention, not found, therefore the claimed invention meets the condition of "novelty". Easy solutions are not implemented to date, demonstrates the consistency of the proposed device the condition of "inventive step".

Thus, the above data suggest that the claimed invention designed for use in experimental and industrial biotechnology, in particular to assess the concentration of retinoid substances in suspension, has the above properties. For the inventive method, it is described in the above-mentioned claims, there are no obstacles to its implementation in practice using common and available funds. Therefore, the claimed invention meets the condition of "industrial applicability".


1. Hakobyan V.B. have been, Bambara M.V., Rahman A.A., Stupin, A., Filatova V.B. have been Ultrasound in the formation of aqueous slurries of refractory biologically active substances // proceedings of the XXII session of the Russian acoustical society session of the scientific the on Board speakers wounds. - M., 2010. - V.3. - S-127.

2. The Stupin, A., Georgians E.V., Nikitina AS, Kubatova O.Y Use of propolis in food emulsions obtained with ultrasound. Food industry, 2010, 2, p.54-56.

3. The Stupin, A., Bambara M.V., Braslavets V.R., Presence AV, Kropachev, GV Aerosol extraction of pine wood resin. Bulletin of the University (Novosibirsk state agrarian University), 2010,4, p.32-39.

4. Hakobyan V.B. have been, David ER, Sveshnikov, I.N., Pashinin AU, Stupin, A. Extraction of resin from pine sawdust. Biotechnology, 2010, No. 2, p.65-69.

5. Bambara M.V., Hakobyan V.B. have been, Sokolov Y.V. Spray method for producing nanoparticles. // Chemical and petroleum engineering. 2009, No. 2, s.

6. The Stupin, A. Suspension of natural resins and retinoid metabolism. M FGNU "Rosinformugol". 2010, 67 S.

7. Lopatin V.N., Priezzhev AV, Apanasenko A.D., Shepelevich N. Century, Lopatin V.V., Pozhilenkova PV, Department IV Methods of light scattering in dispersion analysis of biological systems. M.: FIZMATLIT, 2004, 384 S.

8. Ozerov DU, Afanas'ev M.M. Method for continuous measurement of the concentration of the cellulose fibers in the suspension and the device for its implementation. Patent No. 2067638. Publication date 10.10.1996.

9. Trifonov O.N., Panin MG, Enikeev INCLUDING, Bakulin VP, Electro-hydraulic control concentration of yeast suspension in the wine solution Patent No. 209882. Publication date 10.09.1997.

10. The concentration analyzer magnetic suspension. MA-2, ml?regcus=1&1=0

11. MATIC I. Introduction to chemical equilibrium and kinetics. M, 1984,486 C.

A method of evaluating the concentration of retinoid compounds in aqueous suspension titration involving titration of a suspension of water-soluble and solvent suspended resin like substance with a solvent to reduce light scattering, recorded either visually or with optical instruments that measure the amount of light passing through the liquid.


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