Method of determining maximum concentration of living bacteria
SUBSTANCE: invention relates to biochemistry. Disclosed is a method of determining maximum concentration of living bacteria. A suspension of bacteria with turbidity standard of 5 units is brought to concentration of 500000 mc per 1 ml. Electrical resistance of the suspension is then determined in a direct current field with maximum voltage across open ends of electrodes of 2.8 V. Maximum resistance in the range of 900-1500 kOhm indicates maximum concentration of living bacteria in suspensions of different types of microorganisms.
EFFECT: method provides quality estimation of concentration of living bacteria, which is sufficient for further identification of said bacteria, and cuts analysis time.
1 tbl, 4 ex
The method relates to the field of medicine, namely Microbiology, and is designed to determine the concentration of bacteria in nutrient media.
Improvement of methods for determining the concentration of bacteria in various suspensions is up to date. In the process of cultivation of bacteria in nutrient media is a complex biological process associated with the nutrition of bacteria and accumulation of products of their metabolism. Bacteria fails the process of their dying [Growth and methods bacteria. Pp.62-66. In the book: Medical Microbiology, Virology and immunology / edited ABV. - M., 2004. - 691 S.]. The need to determine the concentration of microbial cells (MC) in suspensions occur when you obtain standard diagnostic, therapeutic and preventive drugs. The diagnosis of infectious disease is put on the basis of determining the genus and species of the pathogen. More effective identification of bacteria in terms of the maximum concentration of living microbial cells during bacterial growth on nutrient media.
There is a method of determining the concentration of microbial cells in the turbidity standard, recording the total number of live and dead microbial cells [Patent RU No. 2285257 C2. Bull. No. 28 dated 10.10.2006,]. This method does not allow to determine the concentration of W is o microbial cells and the timing of their maximum accumulation.
A known method of determining the amount of live microbial cells in culture results in a dense nutrient medium and counting the grown colonies of individual microbial cells [Definition total number of bacteria in the water. S-306. In the book: Microbiology techniques in microbiological research. M, Medicine, 1978, 394 S.], we have chosen as a prototype. However, this method has significant drawbacks: 1) it is impossible to determine the concentration of living microbial cells in the process of cultivation of bacteria in nutrient media; 2) the determination of the amount of live microbial cells in the studied samples can only be obtained after 24 hours of growth of isolated microbial cells on solid nutrient medium (in this period in the study population, usually, ends the process of the death of microbial cells); 3) for the implementation of the method requires the additional costs of the nutrient medium and the time spent specialists Microbiology laboratory (titration study population of bacteria, counting the number of grown colonies of individual microbial cells, mathematical calculation of the concentration microbial cells in the test sample).
The present invention is to develop a rapid method of determining the maximum concentration of living microbial cells in the process of their cultivation on pitt the selected environments. The maximum concentration of live bacteria in suspension refers to the concentration of bacteria, sufficient for the identification of selected bacteria from the patient. Based on the above it follows that the present invention is a qualitative rather than a quantitative estimate of the maximum concentration of live bacteria.
The technical result. The method is based on the electrokinetic properties of microbial cells in a liquid medium and their ability in the field of a permanent electric current having a certain electrical resistance.
Theoretical substantiation of the possibility of determining the electrical resistance of biological particles is reflected in theory after h.helmholtz - Jirina, according to which on the surface of biological particles in a liquid medium, there is the electric double layer condenser, one plate of which is charged surface of a biological particle, and the other ions present in the liquid and carrying the opposite charge. It is established that the bacteria on their surface are defined by the size and sign of the electrical charge. Electrokinetic properties of bacteria may change during the process of food microorganisms, which leads to improvement in electrical resistance of bacteria and demonstrates what isresponse. Based on the study of electrokinetic properties of bacteria Adetokunbo proposed device for the detection of bacteria [A.S. No. 288898, 1971. Bull. No. 7].
The technical result of the proposed method is achieved by the fact that the bacteria in liquid medium in the field of a permanent electric current have some electrical resistance. In this regard, a suspension of bacteria on the turbidity standard in 5 units adjusted to a concentration of 500,000 MK 1 ml and determine in the field of a permanent electric current at a voltage of 2.8 V at open ends of the electrodes, the electrical resistance of an index in the range from 900 to 1,500 ohms indicates a concentration in the suspension of live bacteria in sufficient quantities for identification of bacteria.
The method is as follows. The investigated suspension of bacteria in liquid medium was adjusted to the turbidity standard is 5 units to the concentration of 500000 MK 1 ml of suspension. Received a suspension of bacteria in a volume of 2.0 ml, is placed in the hole plexiglass plate, commonly used in laboratory practice for serological reactions. Using a digital multimeter (Mini digital multimeter) brand M in the limit of measurement 2000 ohms with a resolution of 1 K and an accuracy of ±0.8% of units of account, at the maximum voltage on the electrodes of 2.8 spend In determining the electrical resistance of the bacterial suspension is. When the measure of electrical resistance in the range from 900 to 1,500 ohms registers the concentration of live microbial cells, sufficient for identification of bacteria.
Examples of usage of the proposed method
After 15 hours of growth sporadic culture of Staphylococcus aureus on blood mesopatamia agar (MPA) conducted flush grown colonies of bacteria physiological solution of sodium chloride and the concentration of bacteria increased to 500000 MK 1 ml of standard turbidity in 5 units. Using a digital multimeter (Mini digital multimeter) brand M in the limit of measurement 2000 ohms with a resolution of 1 K and an accuracy of ±0.8% of units of account, when the voltage at the electrodes of 2.8 In the determination of the electrical resistance of the suspended bacteria. Changing the resistance reached 1260 ohms. The concentration of bacteria in the suspension of the results of counting of grown colonies of individual microbial cells (MC) on the solid nutrient medium was 2.4·1012MK 1 ml.
After 12 hours of growth sporadic culture of Proteus mirabilis on blood MPA held flush grown colonies physiological solution of sodium chloride and the concentration of bacteria increased to 500000 MK 1 ml of standard turbidity in 5 units. Using a digital multimeter (Mini digital multimeter) brand M in the limit of measurement 2000 ohms with a resolution of 1 K, and t is the durability of ±0.8% of units of account, when the voltage at the electrodes of 2.8 In the determination of the electrical resistance of the suspended bacteria. Changing the resistance reached 1490 com. The concentration of microbial cells in the suspensions according to the results of calculation of grown colonies on solid culture medium of individual microbial cells was 2.6·1012MK 1 ml.
After 10 hours of growth of hospital strains of culture of Citrobacter freundii in blood MPA held flush grown bacteria physiological solution of sodium chloride and the concentration of bacteria increased to 500000 MK 1 ml of standard turbidity in 5 units. Using a digital multimeter (Mini digital multimeter) brand M in the limit of measurement 2000 ohms with a resolution of 1 K and an accuracy of ±0.8% of units of account, when the voltage at the electrodes of 2.8 In the determination of the electrical resistance of the suspended bacteria. Changing the resistance reached 1115 com. The concentration of bacteria in the suspension of the results of counting of grown colonies of individual microbial cells on solid nutrient medium was 1.4·109MK 1 ml.
After 13 hours of growth of hospital strains culture of Pseudomonas aeruginosa in blood MPA held flush grown bacteria physiological solution of sodium chloride and the concentration of bacteria increased to 500000 MK 1 ml of standard turbidity in 5 units. With SIP is using a digital multimeter (Mini digital multimeter) brand M in the limit of measurement 2000 ohms with a resolution of 1 K and an accuracy of ±0.8% of units of account, when the voltage at the electrodes of 2.8 In the determination of the electrical resistance of the suspended bacteria. Changing the resistance reached 926 com. The concentration of bacteria in the suspension of the results of counting of grown colonies of individual microbial cells on solid nutrient medium was 6.0·1010MK 1 ml.
The method of determining the concentration of live bacteria in the process of their cultivation on nutrient media according to their electrical resistance is introduced on the basis of the division of General Microbiology FGUZ "Center of hygiene and epidemiology in the Tyumen region". The electrical resistance was determined from hospital and sporadic strains of the following bacteria: Pseudomonas aeruginosa, Proteus mirabilis, Citrobacter freundii, Hafnia alvei, Staphylococcus aureus, Enterobacter cloacae. Just spent 48 studies.
Assessment of the economic and positive (significant acceleration of the results of the analysis of the effect of the proposed method performed in comparison with the prototype and presented in the table.
Our proposed method allows the concentration of living microbial cells, necessary for the identification of bacteria isolated from patients, reduces the time of research, does not require large expenditures. The total economic effect on the conduct 100 tests was 343980 rubles.
|Characteristics of a positive effect determine the maximum concentration of live bacteria on the proposed method and prototype|
|Sign||The placeholder||The proposed method|
|The duration of one analysis||24 hours||3-5 minutes|
|Consumption of nutrient media to conduct a single analysis (in rubles)||1250 rubles||The nutrient medium is not required|
|Consumption of laboratory glassware for holding 1 analysis||25 cups for culturing bacteria, 15 germ tubes and 15 pipettes in the amount of 661 RUB||1 hole 72 plexiglass plate|
|Preparation of glassware for analysis||Washing dishes, corking, sterilization||Not required|
|Time spent specialists Microbiology laboratory to conduct 1 analysis||9 hours (1530 rubles)||3-5 minutes|
|The cost of the device for measuring the resistance of suspended bacteria||Not used||120 rubles|
|Total expenses 1 analysis||3441 rubles||120 rubles|
|Expenses 100 research||344100 rubles||120 rubles|
|Laboratory glassware and apparatus for measuring the resistance of suspended bacteria are used repeatedly.|
The method of determining the maximum concentration of live bacteria, characterized in that a suspension of the bacteria on the turbidity standard in 5 units adjusted to a concentration of 500,000 microbial cells in 1 ml and determine in the field of a permanent electric current at the maximum voltage of 2.8 V at open ends of the electrodes, the electrical resistance, the maximum rate of which is in the range from 900 to 1,500 ohms indicates a maximum concentration of live bacteria in suspension.
SUBSTANCE: method consists in examination of hepatic tissue taken by biopsy and analysed for a degree of acidity of hepatic puncture sample by pH-metry. If the pH level is less than 6.76 units, a moderate degree of activity of chronic hepatitis is stated, while the pH variations within 6.76 to 7.25 units - a mild degree of activity of chronic hepatitis, and the pH value exceeding 7.25 units shows chronic hepatitis with minimal activity of a pathological process.
EFFECT: use of the method enables a higher diagnostic degree of activity of chronic hepatitis C and ensures reduced length of assessment and simplicity of implementation.
1 tbl, 1 ex
SUBSTANCE: patient's skin condition is diagnosed by assessing the effectiveness of fibroblast colony-formation, as a parameter describing regeneration cell potential and percentage of dense and diffuse colonies in a cell culture as parameters describing proliferative cell potential. The effectiveness of colony-formation is calculated as percentage ratio of the formed colonies of cell count more than 20 to total explanted cell count. In the other version, a proliferation parameter describing proliferative cell potential is calculated by formula: PP=[1(DC)+2(MC)+3(DenseC)]/100(%), wherein PP is the proliferation parameter; DC is percentage of diffuse colonies, (%); MC is percentage of mixed colonies, (%); DenseC is percentage of dense colonies, (%).
EFFECT: application of the given group of inventions enables diagnosing the condition of fibroblast population of patient's skin.
4 cl, 6 dwg, 6 ex, 3 tbl
SUBSTANCE: antioxidant activity of a broth culture of microorganisms or a cell suspension of microorganisms grown on a solid nutrient medium is evaluated. An oxidable medium is presented by a lecithin solution, while peroxide initiators are as follows: Staphylococcus aureus cells, ascorbic acid, ferric (II) ions (in the form of an aqueous solution of ferric sulphate). Antioxidant activity is evaluated by an ability to inhibit lipid peroxidation by adding concentrated orthophosphoric acid, and 1% 2-thiobarbituric acid in dimethyl sulphoxide or ethanol (1:1). The stained complex is extracted in n-butanol, and after a butanol phase separated, it is spectrophotometered with regard to transmission unit at 550 nm wherein a reference tray is a tray with n-butanol. It is combined with preparing two controls wherein test tubes containing an oxidation substrate - lecithin are added with known antioxidant activity, while in the other one initiated peroxidation is enabled with no antioxidant added.
EFFECT: derived values of the antioxidant status are calculated in units of antioxidant activity.
SUBSTANCE: invention relates to field of medicine, namely to oncology. To diagnose cancer and potential resistance of malignant cells to hypoxia, prepared is cell imprint or suspension of cells, for which conditions of hypoxia are created and spectro-flowmetric analysis of said cells in dynamics of development of process of photodestruction of NAD(P)H fluorescence is performed. Conclusion about presence of tumour cells and their potential resistance to hypoxia is made by increase of NAD(P)H fluorescence intensity in conditions of darkness. Conditions of hypoxia are created by placement of cell suspension or cell imprint between two glasses for cytologic analysis. Spectro-flowmetric analysis is carried out in the range 440-470 nm, with length of excitation wave 365-370 nm.
EFFECT: method makes it possible to diagnose cancer and potential resistance of malignant cells to hypoxia.
3 cl, 6 dwg, 1 ex
SUBSTANCE: method involves preparation of a patient's blood serum sample by drying, grinding and suspending in Vaseline oil; then the sample is examined by infrared spectroscopy in 1200-1000 cm-1 to determine a peak height of absorption bands with maximums 1150, 1130, 1125 cm-1 that is followed by the calculation of the relation of the peak heights with maximum 1150 cm-1 to the peak height with maximum 1130 cm-1 in males and the relation of the peak height with maximum 1150 cm-1 to the peak height with maximum 1125 cm-1 in females. The blood serum sample immediately follows the performed growth surgery, and thereafter in progression, preferentially monthly. If observing increase of the relation of the peak height with maximum 1150 cm-1 to the peak height with maximum 1130 cm-1 in a male patient and the relation of the peak height with maximum 1150 cm-1 to the peak height with maximum 1125 cm-1 in a female patient by the value of 0.5±0.015 as compared to the relation value derived from the previous sample examination, the recurrent cerebral malignant growth is stated.
EFFECT: use of the method enables stating the postoperative recurrent cerebral malignant growth at the early stages.
2 cl, 3 tbl, 3 ex
SUBSTANCE: method for mineral recovery from human connective tissue by low-temperature tissue ignition involving drying of connective tissue kept in 10% formalin in the air for 2-3 days, sample suspension, tissue ignition in porcelain crucibles in an ignition muffle at temperature 450°C for 10-12 hours, suspension of the prepared ignited residue.
EFFECT: method enables effective mineral recovery from human connective tissue.
5 dwg, 1 ex
SUBSTANCE: patient's prostate secretion microscopy and microbiological analysis of sperm are conducted prior to and after three sessions of low-intensity infra-red laser therapy. Further, a final diagnosis of category II, category IIIA or category IIIB chronic prostatitis is stated.
EFFECT: higher differential diagnostic accuracy in chronic prostatitis forms which have essentially different pathogenesis and therapy and improved therapeutic results.
SUBSTANCE: in patients with bronchial asthma performed is iionophoresis with 5% acetylcholine solution in course of laser Doppler fluometry, time of rise and restoration of dopplerogramme on skin of left forearm in point located on medial line 4 cm higher than base of styloid processes of ulnar and radial bones.
EFFECT: basing on obtained data type of microvascular endothelium response is determined as normoreactive-stable, normoreactive-incremental, normoreactive-decremental, hyperreactive-stable, hypereactive-incremental, hypereactive-decremental, hyporeactive-stable, hyporeactive-decremental ot hyporeactive-decremental type of microvascular endothelium response.
10 dwg, 9 ex
SUBSTANCE: reagent for thrombocyte measurement containing Nile blue hydrosulphate as a reagent for thrombocyte staining. A reagents kit for thrombocyte measurement containing Nile blue hydrosulphate as a first reagent, and a buffer solution as a second reagent. A method for thrombocyte measurement wherein Nile blue hydrosulphate is used as the first reagent, and the buffer solution - as the second reagent. A reagent for thrombocyte measurement containing Nile blue and an acid as a staining agent for thrombocyte staining. The reagents kit for thrombocyte measurement containing Nile blue as the first reagent, and a buffer substance as the second reagent. The method for thrombocyte measurement wherein Nile blue and the acid are used as the first reagent, and the buffer substance - as the second reagent.
EFFECT: reagents described above provides higher accuracy of thrombocyte evaluation.
13 cl, 15 dwg, 10 tbl, 48 ex
SUBSTANCE: histologic examination of transverse section is performed through all the layers of arachnoid cyst walls with previous alignment of the piece in paraffin on the edge and study the state of lining and connective-tissue membrane. In case the wall of the cyst is covered in flat arachnoendothelium with hyperchromatic nucleus, the cyst is regarded the non-proliferating, the prognosis is favorable. In case the wall of the cyst is covered with round-shaped arachnoidendothelium with the zones of amplified proliferation of arachnoidendothelium in form of multirowed layer and/or vertical cells alignment, it is regarded as proliferating - the prognosis is unfavorable.
EFFECT: morphologic prediction of children arachnoid brain cyst progression.
1 dwg, 2 dwg, 1 tbl, 2 ex
SUBSTANCE: method for detection of neutrophil extracellular traps in mucosal secretions provides staining of a native preparation 0.1 ml with an equally component mixture 0.1 ml of the Evans blue dye in the concentration of 1:8000 and the Sytox Green dye in the concentration of 1:500, incubation for 5 minutes in the dark. Luminescent microscopy with using filters generating excitation light at wave length max. 490 nm and emission at wave length 520 nm is used to detect neutrophil extracellular traps, living bacterial cells, apoptotic bacterial cells, green cork bacterial cells. The number of neutrophil extracellular traps (%) containing bacteria in 100 counted traps is evaluated.
EFFECT: method enables visualising chromantin, assessing cell viability and accelerating the study.
2 tbl, 3 ex
SUBSTANCE: method involves telomere length measurement by flow-FISH method in cells differentiated by a number of population divisions with a vital stain without preliminary recovery of proliferative cells with using Bis(sulfosuccinimidyl)suberat (BS3) amino group cross-linker.
EFFECT: invention provides the single-step telomere length measurement in cells with a common division quantity.
3 dwg, 1 ex
SUBSTANCE: sample is prepared: an additional weight of nanocarbon forms is dispersed in 1 ml of organic solvents with a degree of polarity smaller than that of water - dimethyl sulphoxide or ethanol. Then it is mixed and exposed to ultrasound for 30 minutes. The prepared nanocarbon suspension is transferred in an aqueous medium to the final concentration of the used solvent 2.5 %. The produced and control samples are added with a viable sensor recombinant luminescent Escherichia coli K12 strain with cloned luxCDABE genes of luminescent Photobacterium leiognathi system. It is followed by incubation for 60 - 180 minutes, measuring luminous intensity and evaluating optical properties of the analysed suspension simultaneously. A toxicity index (T) is calculated with evaluating an actual luminous intensity of the strain (Iact) in comparison with the control of the same concentration of the solvent, considering light absorbing properties of the analysed suspension (D) and an experimental luminescence level of the bacterial luminescent biosensor (Iexp).
EFFECT: invention allows providing higher accuracy and sensitivity of nanocarbon biotoxicity analysis ensured by the introduction of a correction value - the actual luminous intensity of the strain Iact, considering a common factor of emitted light distribution in the analysed suspension.
SUBSTANCE: method involves collecting dust samples with a sterile cotton wool wad from 10-20 cm2 of any surface or the sterile cotton wool filter of a vacuum cleaner. The dust samples are put into a sterile physiological solution, followed by extraction for 30-60 minutes at room temperature while stirring. The supernatant liquid is collected and multiple cultures of the obtained extract are prepared from the liquid. The obtained cultures are sown in a semi-liquid thioglycol medium and modified thioglycol medium obtained by adding defibrinated blood to the thioglycol medium in a given amount. Culturing is performed for 10 days at temperature 37°C and daily visual monitoring, followed by determining the number of microbial cells which are calculated from the McCready probability table, which is compiled based on data on growth properties of microorganisms and tinctorial properties of the bacterial community of the dust sample are determined through microscopic examination of smears of the mixed culture mass which is gram-stained.
EFFECT: simultaneous quantitative determination of the total bacteria number and obtaining cultural, morphological, tinctorial and haemolytic characteristics of the bacterial community of dust samples from buildings.
SUBSTANCE: biosensor contains photo-autotrophic microalgae cells, the fluorescent characteristics of the photosynthesis system of which vary in the presence of cytotoxic chemical compounds in their surroundings: heavy metal ions and herbicides. Cells of green and diatomic microalgae are immobilised in cryogenic gel of polyvinyl alcohol: a cell suspension is deposited on a surface and the cells enter macropores of the polymer carrier under the effect of centrifugal force (5000-14000 g) for 1-10 minutes. A highly sensitive and stable biosensor is obtained based on components taken in the following ratio in wt %: microalgae cells 0.015-1.1; polyvinyl alcohol 7-15; aqueous phase - up to 100. Low concentrations of heavy metals and herbicides in aqueous systems are determined at flow rate of up to 360 ml/h based on the change in the value of relative variable fluorescence chlorophyll cells in the biosensor. The biosensor can be used for a maximum of 60 days.
EFFECT: high sensitivity of the biosensor.
2 dwg, 5 ex
SUBSTANCE: detection and identification of biological objects and their nanocomponents are enabled by exposure to radiation, monochromatic or nonmonochromatic radiation, including laser, sensing of one or more samples containing microobjects and their nanokcomponents with the use of a set of sample response measurement and record devices. The responses characteristics of each radiation conversion event are measured separately or in the aggregate, transferred and reduce in a diagnostically linear form. It is followed normalisation, correction and creation of a base of the reference and diagnosed parametres of microobjects and/or their nanocomponents. Further, the reference, diagnosed and identified microobjects and/or their nanocomponents are recognised and compared with experience data of required parametres on the basis of measurement with the use of a matrix.
EFFECT: use of the declared method allows precise qualitative and quantitative analysis of detected, identified, diagnosed parametres of the microobjects and their nanocomponents on the basis of optical measurement.
4 cl, 12 dwg, 1 tbl, 3 ex
SUBSTANCE: cultivated microbiological objects count is ensured by the measurements of their morphological compositions by determining the size distribution of microorganism cells in a nutrient fluid by variation of scattered light intensity. The fluid flow is sounded with monochromatic coherent light; interaction signal of probe radiation and analysed microbiological objects are recorded by the measurement of amplitude and duration of scattered light pulses by analysed particles; and functions derived from the measurements are plotted in the form of two-dimensional distribution of specified amplitudes and durations expressing statistic parameters of light scattering intensity by particles. After said functions, the size distribution of analysed cultivated microbiological objects and decay products of the nutrient fluid is derived.
EFFECT: higher measurement accuracy due to eliminated error caused by foreign particles that are decay products of the nutrient fluid in cultivation of the analysed microbiological objects.
9 dwg, 5 ex
SUBSTANCE: invention concerns medicine, particularly method of cardiomyocyte selection from cardiomyocyte-containing cell mix without genetic alteration incardiomyocytes. Method of cardiomyocyte content boost in cardiomyocyte-containing cell mix without genetic alteration. Method of cardiomyocyte obtainment without genetic alteration to cardiomyocytes. Method of cardiomyocyte content assessment in cardiomyocyte-containing cell mix.
EFFECT: possible efficient selection of cardiomyocytes from cardiomyocyte-containing cell mix without genetic alterations.
20 cl, 16 dwg, 14 ex
FIELD: chemistry, biochemistry.
SUBSTANCE: method can be used in microbiology, food industry for estimation of viability of unicellular organisms (yeasts, etc.), which demonstrate difference of dielectric properties. Per cent content in mixture of live and dead unicellular microorganisms is determined by deviation of measured value of dielectric permeability from dielectric permeability of mixtures, consisting only of live and only of dead unicellular microorganisms, or by experimental dependence of second derivative of dielectric permeability on humidity.
EFFECT: elaboration of distant methods of assessment in continuous flow, in elaboration of industrial methods of control over live microorganism production.
SUBSTANCE: invention concerns medical microbiology. The method of chronic urogenital gonococcal infection course forecast involves seeding of accompanying fungi of Candida genus in case of gonococcus detection, and persistence factors of accompanying microorganisms is evaluated. If titration of fungi of Candida genus gives not less than 102 colony-forming cells per millilitre and antilysozyme activity evolves simultaneously in the quantity not less than 1.3 mcg/ml per optical density unit, and anticomplementary activity not less than 1.5·106 antilytic complement units, then chronic character of urogenital infection is confirmed diagnosed.
EFFECT: increased accuracy of chronic urogenital gonococcal infection course forecast.
2 ex, 3 tbl
FIELD: microbiology, optics.
SUBSTANCE: invention relates to investigations of materials by assay of their physical or chemical properties using optical devices and to systems wherein material is excited by optical agents resulting to it luminescence. Invention proposes a test carrier as a centrifugal tube. Carrier is separated for upper and bottom cavities by partition. Volume of lower cavity is 0.1 of tube volume. A hole is made in partition near a wall. The constructive decision of partition provides efflux of sample from lower cavity with minimal overcoming the combined forces of wetting and surface tension. Also, invention proposes methods/variants for rapid measurement of absolute concentration of microorganisms in biosubstrate by their photoluminescence. Methods involve using fluorescent or phosphorescent measuring device and above said test carrier. Methods provides increasing rate and precision of assay, to use serial measuring devices and to carry out measurement of the concentration of particles in substrate with another specific gravity value as compared with that of liquid in substrate. Invention can be used in food and biotechnological industry for determination of absolute concentration of microorganisms in different substrates.
EFFECT: improved method for assay, valuable properties of carrier.
2 tbl, 1 dwg