Method for preparing sorption material of blastemas

FIELD: medicine, pharmaceutics.

SUBSTANCE: invention concerns pharmaceutical industry, in particular to a method for preparing sorption material of blastemas. The method for preparing sorption material of Cladonia blastemas to be ground, mechano-chemically activated at one stage under certain conditions.

EFFECT: sorption material prepared by the method described above shows high sorption activity.

4 dwg, 3 tbl

 

The invention relates to biotechnology (biotech) industry, namely to obtain the sorption material for medical purposes (therapeutic, prophylactic) from vegetable raw materials.

One of the main areas of biotechnology involves the development of sorption materials and their application in medicine and the medical industry as indispensable materials for enterosorption. Method of enterosorption began to actively compete with hemosorption in connection with a number of advantages: no need of surgical intervention and the risk of possible complications specific to hemosorption; no direct damaging contact with biological liquids (blood, lymph); and the widespread use of sorption therapy for outpatient treatment in the field and in the ordinary home.

Analyzing the requirements of regulatory documents and clinical practice requirements chelators, you can select a set of inherent characteristics of the "ideal" enterosorbent:

- full safety and toxicity;

- high biocompatibility with human tissue, blood and other biosubstrate of the body;

- undamaged action on the mucous membranes of the oral cavity, esophagus, gastrointestinal tract;

- voters the other sorption medium molecular toxic metabolites;

- high adsorption capacity.

The problem of creating safe and effective enterosorbent, designed to cleanse the body from toxic substances, which are produced in various diseases, for many years to solve scientists from different countries.

Known chelators can be combined into the following groups.

1. Carbon adsorbents based on activated carbon and carbon materials (activated carbon, carbolenum, Gastronorm, Inderal, Carbogen, actilon and others).

2. Ion-exchange materials (cholestyramine).

3. Chelators on the basis of lignin (lignosol, profepa).

4. Derivatives of polyvinylpyrrolidone (intersorb, antinodes).

5. Sorbents on the basis of natural minerals (white clay, silica minerals, zeolites).

6. Chelators of plant origin (seeds of the plantain, the guar gum, marshmallow goo, chicory root, pumpkin seeds, dietary fiber, bran cereal, pectin).

Chelators of the latter group have a number of advantages: does not have an irritating effect on the gastrointestinal tract, do not cause adverse and toxic effects, harmless at their long reception (for example, see EN 2389498, CL AC 36/185, 2009).

A method of obtaining enterosorbent of plant origin (see EN 2391998, CL AC 36/902, 2008), provide the Commissioner in the form of granules, contains Sphagnum fuscum obtained by grinding the dried turf Sphagnum fuscum to a particle size of 0.1-0.3 mm, followed by mixing with 10% aqueous low-molecular medical polyvinylpyrrolidine, granulating, drying, and re-granulation.

A common feature of the claimed invention and the well-known analogy is the use of local plant materials. The drawbacks can be summarized as follows: low biological value of the obtained sorbent, low adaptability of the process, expressed in costly and time-consuming process of obtaining chelators, which, in particular, may consist of several stages, such as dissolving in alkalis, neutralization, washing with water, multi-stage drying, thickening of suspensions and other

Task to be solved by the claimed invention is directed, is expressed in the expansion of the range of the sorption materials of vegetable origin and methods for their production, improve their sorption activity.

As the source to create sorption materials used thallus of lichens of the genera Cladonia moss.

The use of lichens as food product is known for a very long time, there is an assumption that the biblical manna was the lichen Aspicilia edible (Aspicilia esculenta), free-living wandering iSync, common in desert areas. Pounded the thalli of lichens slightly reminiscent of flour, and baked from this flour unleavened cakes quite edible. In Japan lichen as a delicacy is put in soup or salad, and also in North America, many species are used as food. From the thalli of lichens of the genus Cladonia cooked jellies, jellies, and was used as an additive to flour. Lichens Cladonia spp., Cetraria islandica today are used for alcohol production.

Lichens of the genus Cladonia - natural medicinal and food raw materials used in folk medicine for treatment of many diseases. In the thalli of lichens are non-hormonal physiologically active substances (NFAW) different groups: carbohydrates (70-80%) in the form of lichen β-polysaccharides of lihenina and saligenin (1); tannins (1-2%); lichen acid (2-3%) - Lecanora, fistula, uninova (2); trace elements (raw materials, the content of heavy metals is much smaller MPC). The content in the thallus of lichens PAV causes quite widely used in official and folk medicine to treat diseases of the gastrointestinal tract, dystrophies, General exhaustion, diseases of the respiratory tract and lungs, infectious skin diseases, burns and diaper rash, disorders of the thyroid gland and other endocrine diseases, also p is modify them as immunomodulatory, cancer, hepato-protective and detoxification of drugs. Found Lichonin and saligenin inhibit animals the growth of some tumors (see Filippov CENTURY, Pavlov N., Shashurin M.M., Kershengoltsa BM Influence of biologically active substances from the thalli of lichens of North, extracted by various methods, the biological properties of Mycobacterium tuberculosis // Siberian journal of medicine. 2008. No. 3. S-103).

However, as β-polysaccharides, β-glycosidic bonds in which practically is not broken down in the gastrointestinal tract, themselves lichen carbohydrates almost not absorbed by the body. The task is to process pre-treatment of lichen thalli to increase the bioavailability of these components. This problem is solved in the framework of the proposed method by mechanochemical activation.

The technical effect of the invention is that by mechanochemical treatment of thalli of the lichen significantly increased sorption activity towards medium - and low-molecular-weight toxins, cations of heavy metals due to the formation of lichen β-oligosaccharides from natural polysaccharide of lihenina, also increases the biological activity NFAW, for example, lichen acids (figure 2) in mechanochemical preparation "Agel the-sorbent".

To solve this problem the method of obtaining sorption material from the thalli of lichens by mechanochemical processing of dry thalli of lichens differs in that it includes a flow solid-phase mechanochemical reactions without solvents in one technological stage, with mechanochemical activation are dry lichen thalli, pre-crushed to 1-3 mm, at a speed of 1200-1500 rpm for 1-3 minutes in the air in the mill-activator of the planetary type. The resulting powder has a sorption material "Moss-sorbent". Formed by mechanical activation of the thalli of lichens β-oligosaccharides, which cause increased sorption activity to low and medium molecular toxins and heavy metal cations, increasing sorption activity contributes to the increase in specific surface area and reducing the particle size of the sorbent (figure 3). The presence of solid-phase preparation of "Moss-sorbent" natural PAV (lichen acid mild antibacterial action, vitamins, trace elements) does not affect its sorption qualities, and give him additional therapeutic and prophylactic properties. Preliminary mechanical activation of moss with getting solid powder "Moss-sorbent can significantly reduce the particle size to between 10 and 100 nm, to simplify the technology of processing of vegetable raw materials and to expand the range of sorption materials from vegetable raw materials high adsorption activity with a wide spectrum of action.

Comparative analysis of the characteristics of the claimed solution with signs analogues demonstrates compliance of the claimed solution to the criterion "novelty".

The invention consists in that the mechanically activated drug "Moss-sorbent has a high specific surface area, good adsorption medium and low molecular weight toxins, which, in particular, include protein toxins and heavy metal cations. The obtained sorption material has a high absorption capacity, which is associated not only with a developed surface, but with the increase in the number of functional groups (figure 4), this allows you to bind and remove exogenous and endogenous toxins (cholesterol, bilirubin, products of fat cells and others). Also there is a mild bactericidal action of lichen acids, which contributes to a healthy microflora due to lysis of pathogenic and conditionally pathogenic bacterial cells and sorption their metabolic products.

The invention can be implemented as follows.

Dry lichen thalli, pre-crushed to 1-3 mm, podvergaut the mechanochemical activation without the presence of solvents in one technological stage at a speed of 1200-1500 rpm for 1-3 minutes in the air in the mill-activator planetary type, shock-abrasive mode which provides the maximum impact of the grinding media on the subject. The resulting powder sorption material "Moss-sorbent".

Mechanochemical activation lichen raw materials increases the bioavailability of the components in the gap part of the chemical bonds (such as steady as a β-glycoside) and obtain oligosaccharides from natural polysaccharides. The successful result of mechanochemical processing bioserie is the increase in the product over the full range PAV in biologically available (water soluble) forms (figure 4), high adsorption activity, especially in the first 20 minutes (table 2, 3).

To study changes in the composition of lichen thalli with solid-phase mechanochemical processing using the method of IR-spectroscopy, the change of the surface topography was studied using atomic force microscopy. To determine the bactericidal properties of the obtained sorption material "Moss-sorbent used for microbiological testing. The efficiency of the adsorption properties were studied by direct spectrophotometry.

The study of powder moss, obtained by solid-phase mechanochemical technology in one stage, without the use of solvents, the method of IR spectroscopy showed that the nature of the IR spectra of all samples of the gel were identical in presence of characteristic functional groups (see figure 4). The set of peaks of absorption does not vary depending on treatment conditions. However, the intensity of all observed absorption bands depends on the method of obtaining a sample of a biological product. The increase in absorption intensity in the region of stretching vibrations of the Oh-group (3450-3350 cm-1) mechanochemical moss indicates rupture of rugged β-glycosidic linkages in the source insoluble polysaccharides included in lichen raw materials, and as a consequence, education is more bioavailable β-oligosaccharides.

The presence in the IR spectrum of a number of bands (1670 1630 cm-1- OS communication conjugated systems; 1200-1270 cm-1-C-O-C - group atoms; 1100-1000 cm-1as pyranose cycles, and-S-OS - group atoms; 900 cm-1- deformation C-H) above for the mechanically activated sample, indicating a greater saturation of its functional groups and the increase in the content PAV in the study of mechanochemical the moss.

The antibacterial properties of the drug, "the Moss-sorbent was determined in vitro on cultures of bacterial strains four conditionally pathogenic and pathogenic microorganisms by standard and modified methods.

Comparative study of antibacterial action of lichen thalli coarse without mechanochemical processing (powder No. 1 control) and studies the target of the drug, "the Moss-sorbent" on the following cultures by the standards of the turbidity of the cultures at 5 and 10 units:

1. Staphylococcus aureus - 6538-p

2. Escherichia coli - H-257

3. Pseudomonas aeruginosa - 33105

4. Klebsiella pneumoniae

Used standard culture medium: medium Endo, milk-yolk salt agar, Wednesday Ploskirev, mastopathy agar (MPA), which were prepared according to standard recipe (MUK 4.2.1890-04. Determination of the sensitivity of microorganisms to antibiotics. Guidelines, approved by chief state sanitary inspector of the RF 04.03.2004 g) adding to the culture medium of the control and the sample amount of 5.0 mg/ml. Lawn method was seeding of microorganisms and after cultivation thermostat at 37,0°C was estimated by the intensity of their growth. The evaluation results are shown in table 2.

Powder No. 1 showed either a subtle attempt to lysis, or the microorganisms were visualized partially. Therefore demonstrated higher efficacy "Moss-sorbent" in relation to conditionally pathogenic and pathogenic bacterial strains, including Staphylococus aureus, Klebsiella pneumoniae and other

The formation of new structures and a larger specific surface area was recorded by atomic force microscopy (figb), while the flat surface of the powder lichen coarse differed by the absence and presence of smooth relief (figa).

the step mechanochemical sorption material "Moss-sorbent proved in experiments with marker medium - and low-molecular-weight toxins - methylene blue (table 2) and with a cation With2+(table 3). Adsorption activity of powders of moss on methylene blue was determined by the method recommended in GOST 4453-74, but changes have been made to simulate conditions close to the gastric. Gastric environment was created by acidification with Hcl for 30 mm at t=37°C. For research use spectrometer software "LAMBDA 20" when the wavelength (λ) 400 nm in cuvettes thick light absorbing layer 10 mm relative to distilled water. In addition, changes have been introduced during centrifugation. In this case, the centrifugation does not allow complete separation of the phases, so the separation of the phases is additionally carried out by filtration. For this he used a Buchner funnel and filter paper (GOST 12026-76) of the same size. Statistical processing of the research results was performed using the software package Microsoft Excel 2010. The data are processed statistically standard parametric methods of calculation of the average value, standard deviation, the confidence interval for the T-test (n=9<20) at a significance level of p=0.05. The results are presented as M±m, where M is the arithmetic mean value, m is the confidence interval. Sorption daily capacity of the mechanically activated samples is SCA was 48±3 mg/g It should be noted that this is a very high performance compared with the known adsorbents such as polifan and white clay, adsorption capacity which methylene blue is 10.0 and 11.4 mg/g, respectively (Markelov D.A., Nizak O.V., Gerashchenko I.I. Comparative study of the adsorption activity of medical sorbents // pharmaceutical journal. 2008. V.42. No. 7. P.30-33., Reshetnikov V. Evaluation of adsorption capacity of chelators and their dosage forms // pharmaceutical journal. 2003. V.37. No. 5. Pp.28-32). The rate of absorption is also higher in mechanically activated drug in the first 20 minutes was adsorbirovannoi 92% of its daily adsorption capacity, unlike moss, coarse grinding, where over the same period of time, the adsorption capacity was 2 times lower than that of the mechanically activated sample, and was only 69% of daily activity.

Previously it was shown that the natural lichen β-oligosaccharides act as the coordination complex with metal cations (Anshakov V.V. Mechanochemical technology of biologically active compounds from lichens / Rev, Bmerrill, Eshley, Aey // proceedings of Samara scientific center of Russian Academy of Sciences. 2011. Vol.13, No. 1. P.236-240). The adsorption capacity for heavy metals was determined on the sample ions With2+from the conventional solutions of cobalt chloride (II). From table 3 it can be seen that the sorption activity of the material, Moss-sorbent 2.5 times higher than the sorption properties of the powder lichen coarse.

Presents the sorption material based on mechanically activated thalli of lichens expanding the raw material base for the adsorbents of plant materials and can be used to create chelators to form healthy microflora, treatment of exogenous and endogenous intoxications of various etiologies.

Table 1
Antibacterial action of the powder 1 and the sorption material "Moss-sorbent" culture of conditionally pathogenic and pathogenic bacterial strains
Name types of bacterial strainsAntibacterial action of the powder No. 1(control)Antibacterial action of the drug, "the Moss-sorbent"
Staphylococcus aureus - 6538-p+*)++++
Escherichia coli - H-257++++
Pseudomonas aeruginosa - 33105+ ++++
Klebsiella pneumoniae+++++
Ex.:*)+ - weak lysis; ++ - partial lizirovania; +++ - almost complete lysis; ++++ - complete lysis

Table 2
Adsorption active powder lichen indicator methylene blue, mg/g
The sorption materialThe adsorption time
20 min24 hours
Powder lichen coarse27±339±2
Powder moss mechanically activated "Moss-sorbent"44±448±3

Table 3
Specific adsorption of ions of cobalt (II), mmol/kg
The sorption materialThe concentration of Co2+mol/l
0,2 0,40,60,8
Powder lichen coarse10153560
Powder moss mechanically activated "Moss-sorbent"257080150

The method of obtaining the sorption material from plant material, characterized in that the dried thallus of lichens of the genus Cladonia, chopped 1-3 mm, is subjected to mechanochemical activation in one technological stage at a speed of 1200-1500 rpm for 1-3 min in air in the mill-activator of the planetary type.



 

Same patents:

FIELD: medicine, pharmaceutics.

SUBSTANCE: invention refers to chemical-pharmaceutical industry, medicine and veterinary science, particularly new drugs for inflammatory diseases of various geneses. What is presented is a pharmaceutical composition possessing anti-inflammatory, immunotropic, antiallergic and wound healing action which represents a solution for parenteral application and contains peptides associated with phospholipids prepared from cod liver, a wedding agent, sodium chloride, a preserving agent and water for injections. The composition may additionally contain ethanol in the amount of 1.0-15.0 wt %, an antioxidant in the amount of 0.01-0.5 wt % and a buffer in the amount of 0.1-5.0 wt %. The pharmaceutical composition has no side effects inherent in previously known drugs used for the same application.

EFFECT: use allows eliminating the application of steroid and non-steroid anti-inflammatory drugs or reducing the doses substantially.

8 cl, 15 ex, 7 tbl, 1 dwg

FIELD: medicine, pharmaceutics.

SUBSTANCE: invention refers to pharmaceutics. A method of treating chronic recurrent oral ulceration by coating an injured oral mucosa with a therapeutic agent containing methyl cellulose A15CPH, arabinogalactan containing nanosized zero-valent 18% silver 7-16 nm and water for injections taken in certain proportions with the compound applied 3-6 times a day and combined with prescribing diquertin 0.06 g 3 times a day and fibrolar C 0.3 g 3 times a day. The agent for chronic recurrent oral ulceration used in the method described above.

EFFECT: declared method and agent enable higher clinical effectiveness, prolonged remission, reduced length of treatment.

2 cl, 2 ex

FIELD: medicine.

SUBSTANCE: invention is applicable in veterinary science for treating mastitis in animals. A tylosin agent for treating mastitis in animals additionally contains glycerol, Carbopol, triethanol amine, fir essence in the following proportions, %: tylosin 0.05-1.0; glycerol 5.0-10.0; Carbopol 0.15 -0.20; triethanol amine 0.02-0.03; fir essence 0.1-0.3; water up to 100. The agent shows a good wound healing effect, creates a thin surface film, soothes skin, possess antiseptic properties, ability to scare insects, flies in particular.

EFFECT: invention provides higher preventive effectiveness of the agent and lower cost price of animal treatment.

3 tbl, 3 ex

FIELD: medicine.

SUBSTANCE: invention refers to biotechnology and medicine and represents a method for making a resorbed polylactide matrix for cell culture and implantation for wound healing that involves preparing a resorbed hydrophilic porous polylactide matrix uniformly coated with type 1 microfibrillar collagen; for this purpose the hydrophilic porous polylactide matrix 13 mcm tp 15 mcm thick with the pore diameter of 2 mcm to 3 mcm is prepared and then coated with 0.01% type 1 collagen in 0.1% acetic acid, incubated for 30 minutes at room temperature; thereafter, the matrix surface is washed in phosphate-buffered saline pH 7.4 to remove protein not bound with a substrate; the procedure of coating with the type 1 collagen solution is performed for two more times; the hydrophilic surface of the porous polylactide matrix uniformly coated with microfibrillar collagen structures of the diameter of 10 nm to 20 nm is applicable for human keratinocyte culture.

EFFECT: invention provides more effective structure of the protein layer of the matrix, simplified and cheaper method, and reduced resorption time of the polylactide matrix.

4 cl, 3 ex, 6 dwg

FIELD: medicine.

SUBSTANCE: what is offered is using cyproheptadine as an agent preventing developing disorders in pulmonary tissue and blood system caused by the introduction of cytostatics. It is shown that cyproheptadine prevents alveoli infiltration, oedema of interalveoral septum, connective tissue development in lungs with reducing blood lymphocyte count and peripheral blood and bone marrow neutrophilic granulocyte count.

EFFECT: invention may be used for pharmacological correction of pulmonary fibrosis and blood system disorders developing with prescribing anticancer preparations.

1 dwg, 1 tbl

FIELD: medicine.

SUBSTANCE: invention relates to medicine, namely to oncology, and concerns prevention of purulent-septic complications after radical mastectomy. For this purpose against the background of generally accepted complex therapy hypodermically at the level of vertebras Th II - Th VI medical mixture in composition: local anesthetic 1 ml, glucose 40% to 1 ml, 0.9% solution of sodium chloride 4 ml, glutoxim 3% 2 ml, is introduced. Procedure is carried out five times -a day before operation, on the first day after operation and then three times with 24 hour interval.

EFFECT: method ensures reduction of purulent-septic complication frequency as a result of efficient reduction of terms of lymphorrhea duration and correction of local immune-inflammatory reaction.

4 tbl, 1 ex

FIELD: medicine.

SUBSTANCE: invention relates to field of veterinary. Medicine for treatment and prevention of postnatal endometritis and syndrome of metritis-mastitis-agalactia in sows, contains the following components, wt %: Florfenicol - 1.0-3.0, lincomycin hydrochloride - 1.0-3.0, Dimethylsulfoxide - 10.0, Glycerin - 10.0, 1.2 propylene glycol - 10.0, Sunflower refined oil - 12.5, emulsion wax -1.0, stearic acid - 0.9, Water for injection - to 100%.

EFFECT: application of medicine makes it possible to increase efficiency of treatment and prevention of postnatal endometritis and syndrome of metritis-mastitis-agalactia in sows.

17 tbl, 5 ex

FIELD: medicine.

SUBSTANCE: invention relates to medicine, namely to diseases of locomotive apparatus and can be applied for treatment of acute or chronic tendovaginitis. Ultrasonic scanning (US) of inflamed synovial bursas is performed to patient. During examination under local anesthesia with solution of 2% lidocaine puncture of bursa and suction of content with the following inoculation on medium for verification of causative agent and sensitivity to antibiotics are performed. Synovial bursa is washed with solutions of antiseptics with following introduction into its cavity of target antibiotics, dissolved in 1% solution of novocaine. Then, after thirty minutes after achiving anesthetic effect of novocaine absorption, patient performs flexion-extention movements. Procedure is carried out two times per day with interval in twenty hours.

EFFECT: claimed method of tendovaginitis treatment is simple in application, reduces temporary disability, has strongly pronounced cosmetic effect and can be recommend to wide range of practicing doctors of hospital or clinic.

1 tbl

FIELD: medicine.

SUBSTANCE: invention relates to medicine, namely to therapy, physiotherapy, balneology. Method includes carrying out respiratory gymnastics, inhalations with mineral water, manual massage of chest, applications of peloids, electric stimulation at the background of drug basic therapy. During electric stimulation impact is performed by high-amplitude pulse current with biological feedback, with frequency 90-100 Hz and gradually increasing current intensity until sensation of painless vibration appears. On the first day impact is performed paravertebrally at the level D1-D7 and along 7-8 intercostal spaces. On the second day impact is performed paravertebrally and on collar area. Peloid therapy is carried out 20-30 minutes after electric stimulation. In first application impact is performed on chest at temperature 38-40°C and 20-30 minute exposure. On the second day of application impact is performed on collar area at temperature 35-36°C. Course of treatment includes 6-7 coupled impacts on each zone.

EFFECT: method increases treatment efficiency and increases remission duration due to expressed local and systemic anti-inflammatory effect.

2 tbl, 2 ex

FIELD: chemistry.

SUBSTANCE: invention relates to a crystalline form of ilaprazole hydrate (2[[(4-methoxy-3-methyl-2-pyridinyl)-methyl]sulphinyl]-5-(1H-pyrrol-1-yl) 1H-benzimidazole). The invention also relates to a pharmaceutical composition for inhibiting gastric acid secretion, as well as a method of treating inflammatory gastrointestinal disorders.

EFFECT: novel crystalline form of ilaprazole hydrate is obtained.

7 cl, 21 dwg, 19 tbl, 5 ex

FIELD: medicine, pharmaceutics.

SUBSTANCE: invention refers to chemical-pharmaceutical industry and may be used in sports medicine, as well as for prevention and correction of the disorders caused by the body exposure to stress and other unfavourable environmental factors. A composition showing restorative, general tonic and antidepressant action contains ginseng root extract, L-glutamine, eleuterococcus extract, native royal jelly, taurine, guarana extract, licorice root and rhizome extract, zinc gluconate, fructose, citric acid, a preserving agent, a sweetening agent, an aromatiser and water.

EFFECT: composition provides good bioavailability and wide spectrum of therapeutic action.

2 ex

FIELD: medicine.

SUBSTANCE: invention relates to medicine, namely to oncology, and can be applied for fluorescent diagnostics of malignant neoplasms. By means of light source with wavelength 385-469 nm boundaries and character of fluorescent nidus of lymph nodes are estimated after I level lymphadenectomy. If bright red glow of entire volume of lymph node or fluorescence in form of nidus inside lymph node is present, its metastatic affection is diagnosed. Fluorescence in form of "scalloped" rim on the border of lymph node indicates absence of metastatic affection.

EFFECT: invention makes it possible to prevent total lymphadenectomy due to intraoperative detection of metastatic affection of lymph nodes.

2 ex

FIELD: medicine.

SUBSTANCE: invention relates to chemical-pharmaceutical industry and represents method of determining cosmetic product emulsion stability, characterised by the following: selection and preparation of samples are carried out and centrifuged and it differs by the fact that at the first stage carried out are microscopic observation of centrifuged material and calculation of number of micelle n on site 1 cm2 at 10, or 40, or 100-multiple magnification with the following comparison with the number of micelles n0 in sample, which is not subjected to preparation, considering emulsion conditionally stable at and at the second stage samples with conditionally stable emulsion, are subjected by accelerated aging during a month by placing of examined samples in thermostat and keeping at temperature 40-42°C and relative humidity 65±2%, periodically carrying out microscopic observation of samples on 1 cm2 site at 10, or 40, or 100-multiple magnification and statistic processing of experimental data with construction of mathematical model of forecast and solution of equation of form where n' - is number of micelles, pieces./cm2; τ is time of accelerated aging, days; design factors of exponential equation, obtained in processing experimental data, at τ → given time of storing, with calculation of possible emulsion state, in case of value emulsion is considered stable and suitable for durative storage, and in case of it is considered unsuitable for durative storing.

EFFECT: invention ensures increased effectiveness of determination of emulsion stability.

2 ex, 2 tbl, 6 dwg

FIELD: food industry.

SUBSTANCE: invention relates to a biologically active composition and may be used as a remedy with geroprotective activity. The composition contains a concentrate of vegetal saponins - 0.5-5.0 weight parts, Ala-Glu-Asp-Gly / Ala-His peptides composition - 0.001-0.01 weight parts, a complex of acetyl-L-carnitine and alpha lipoic acid - 10.0-25.0 weight parts. The composition with preventive and corrective action is orally administered in an amount of 140-300 mg per day during 28-30 days.

EFFECT: biologically active substances composition stimulates production of dehydroepiandrosterone, reconditions epiphysis functions and increases melatonin level, protects against oxidation with mitochondrion free radicals.

8 cl, 6 ex

FIELD: chemistry.

SUBSTANCE: invention can be used in cosmetic industry when producing preparations which protect the skin from UV radiation. The finely dispersed titanium dioxide-based composite contains finely dispersed titanium dioxide particles which are combined with one or more polymers containing constituent monomers in form of carboxylic acid and/or a carboxylic acid derivative represented by the formula , where R is a C1-C15 alkenyl group, wherein hydrogen atoms can be substituted with a carboxyl group or a hydroxyl group; and X is a hydrogen atom or an alkali metal or a polyoxyethylene or polyoxypropylene group with 1-12 bonded moles. The average peak width of the maximum diffraction intensity assigned to titanium dioxide crystals is 2.0° or less in X-ray powder diffraction analysis.

EFFECT: invention increases transparency and stability of finely dispersed titanium dioxide-based composite, UV radiation absorption capacity thereof and re-dispersion capacity thereof.

11 cl, 9 tbl, 12 ex

FIELD: medicine.

SUBSTANCE: invention refers to medicine, particularly to methods for preparing 68Ga solutions which involves the following stages: reaction of 68Ge/68Ga generator eluate and cation-exchange resin, washing of cation-exchange resin in the mixture of 0.2-1 M hydrochloric acid and 20-80 vol. % of acetone, eluation of 68Ga with cation-exchange resin by the mixture of 1.8-2.5 M hydrochloric acid and 20-80 vol. % of acetone, reaction of the prepared eluate and anion-exchange resin, washing of anion-exchange resin in an organic solvent, drying of anion-exchange resin by air and neutral gas and eluation of 68Ga with anion-exchange resin by an aqueous solution of 0.01-0.1 M hydrochloric acid wherein it's amount is 15-25 less than that of the initial 68Ge/68Ga generator eluate. EFFECT: method provides preparing the concentrated solutions of the 68Ga radionuclides of high chemical and radiochemical purity containing no organic solvents that enables preparing the 68Ga radiopharmaceutical of high molar activity and radiochemical purity.

2 cl, 3 tbl, 2 dwg, 4 ex

FIELD: medicine.

SUBSTANCE: herbal tea for prevention of primary placental insufficiency in the patients suffering local immunodeficiency manifestations refers to medicine, namely obstetrics and gynaecology, and may be used in a complex preconception preparation of the women suffering fertility disorders, preferentially inflammatory ones, with clinical local immunodeficiency manifestations in maternity welfare centres. The declared tea contains morning and evening herbal teas.

EFFECT: herbal tea has a number of advantages: non-invasiveness, low price, ease-to-use, no side effects that enables higher effectiveness of prevention of primary placental insufficiency, avoided pregnancy and birth complications, improved perinatal indicators with low drug load on the woman's body before and during pregnancy.

3 ex

FIELD: medicine.

SUBSTANCE: herbal tea for prevention of primary placental insufficiency in the patients suffering local immunodeficiency manifestations refers to medicine, namely obstetrics and gynaecology, and may be used in a complex preconception preparation of the women suffering fertility disorders, preferentially inflammatory ones, with clinical local immunodeficiency manifestations in maternity welfare centres. The declared tea contains morning and evening herbal teas.

EFFECT: herbal tea has a number of advantages: non-invasiveness, low price, ease-to-use, no side effects that enables higher effectiveness of prevention of primary placental insufficiency, avoided pregnancy and birth complications, improved perinatal indicators with low drug load on the woman's body before and during pregnancy.

3 ex

FIELD: medicine.

SUBSTANCE: herbal tea for prevention of primary placental insufficiency in the patients suffering local immunodeficiency manifestations refers to medicine, namely obstetrics and gynaecology, and may be used in a complex preconception preparation of the women suffering fertility disorders, preferentially inflammatory ones, with clinical local immunodeficiency manifestations in maternity welfare centres. The declared tea contains morning and evening herbal teas.

EFFECT: herbal tea has a number of advantages: non-invasiveness, low price, ease-to-use, no side effects that enables higher effectiveness of prevention of primary placental insufficiency, avoided pregnancy and birth complications, improved perinatal indicators with low drug load on the woman's body before and during pregnancy.

3 ex

FIELD: medicine.

SUBSTANCE: invention refers to veterinary medicine. A method of treating (therapy) subclinical mastitis in cows involves the introduction of the St. John's wort tea 15 ml in the involved lobes of mammary gland three times every 24 hours combined with the single intramuscular introduction of the stress corrector Ligfol 5 ml.

EFFECT: invention provides higher clinical effectiveness.

FIELD: medicine, oncology, amino acids.

SUBSTANCE: invention relates, in particular, to the development of an antitumor preparation based on natural substances. Invention relates to an amino acid preparation comprising at least one modified essential amino acid obtained by treatment of amino acid by ultraviolet radiation (UV) at wavelength 250-350 nm for 12-80 h at temperature 15-30oC or with ozone at temperature 15-25oC. The modified amino acid has no toxicity for health cells. Also, invention relates to a method for preparing such preparation. Invention provides the development of an antitumor preparation based on modified amino acids and expanded assortment of antitumor preparations being without cytotoxicity for normal cells.

EFFECT: valuable medicinal antitumor properties of preparation.

8 cl, 4 tbl, 2 dwg, 4 ex

Up!