Method of estimating functional reserves of human organism
SUBSTANCE: invention relates to field of medicine, namely to functional diagnostics, and can be used in outpatient clinics, centres of preventive medicine, sanatoria, as well as in rooms of rehabilitation medicine in form of computer programme for diagnostics. Estimation of parameters characterising functional state of cardiovascular system, physical state, biochemical status, psychological state, as well as health self-estimation is performed. Values of each parameter, corresponding to the norm, are converted into a single ten-point four-level scale. After that, obtained individual values of each parameter are converted into ten-point scale. On the basis of obtained for each individual parameter values, determined is integral index of: health self-estimation, functional state of cardio-vascular system, physical state, biochemical status, psychological state. Obtained in ten-point scale values of integral indices, which characterise functional state of human body: health self-estimation, functional state of cardio-vascular system, physical state, biochemical status, psychological state are compared with the intervals. Conclusion about their unsatisfactory, satisfactory, good or excellent state is made.
EFFECT: method makes it possible to give quantitative estimation of functional reserves in single integral four-level scale, quantitatively estimate risks of somatic disease development, form conclusion with recommendations on health improvement for people of risk groups and prevention of respective diseases.
1 tbl, 3 dwg
The present invention relates to the field of medicine, namely functional diagnostics, and can be used in clinics, centers of preventive medicine, health centers, and offices of regenerative medicine in the form of a computer program to diagnose.
One of the most important tasks in the sphere of protection of human health is the development of techniques for evaluating the functional reserves of the organism and algorithms to identify individuals at-risk groups for the development of common diseases. Indeed, the enormous social role of valuation reserves (resource) health and monitoring for early detection of dysfunctions associated with social and productive human activity and the prevention of development of pathological process. General requirements for monitoring the health status of the population come from the fact that it should cover the different occupational and age groups, to be aimed primarily at maintaining optimal functional state of the person and to ensure the active prevention and restorative correction of detected malfunctions (Razumov A.N., Bobrowniki I.P., 2010).
There is a method of assessing the physiological status of "Health Express", designed to assess the level psychophysiol the practical and physical health, reserves of the body, parameters of physical development and issuance of personal recommendations for adjustment of status and lifestyle choices on the basis of measurements of parameters of physical development, measurement speed, visual-motor reaction, and analysis of heart rate variability.
Principle: a hardware-software complex "Health Express" consists of a system control stress levels, a computer program to assess the level of health "Rhythm Express" by analysis of heart rate variability and mathematical models of the processes of development of the organism, the set of devices for measuring physiological parameters. The software includes software for generating and maintaining the database for the interpretation and conclusion of the survey results and their subsequent display and printing (Orlova N.V., Chukaeva I.I. Organization and operation of health centers. The tutorial): GOU VPO RSMU, 2010, S.11-12).
The known method of complex assessment of the functional state "Effect". The system is designed for solving problems of diagnostics of disorders of the functional state of the human operator (with the help of specially adapted for this purpose automated tests), the construction of pivot of the numerical assessments of functional status on aggregate heterogeneous information, the Veda is s electronic medical record, choice of remedial measures identified in the individual readings.
Automated Advisory system "Effect" includes the following functional subsystems: system database management system; expert assessments of the effectiveness of remedial actions by the main groups of diseases. The knowledge base of the proposed system uses an expert approach to assessing the effectiveness of Spa treatment, which is based on an objective assessment of the dynamics of the patient's condition and is expressed in points. The parameters characterizing the norm, and the absence of pathological changes is estimated at 1 point. The degree of change, estimated at 20 points, marked by the States, not indicated for treatment in the sanatorium, but can occur during exacerbations of the disease. The maximum number of points corresponds to a larger change of the indicators, the minimum is the lowest or no change. For each patient filled card account of the effectiveness of treatment, which highlights the presence (if the mean clinical and radiological data) or value (for laboratory and functional data) indicators and considered their evidence in points. Fueled amount of points, while the number of considered indicators should be consistent and put in special the ones graphs. The same is done after the end of treatment. The efficiency ratio is determined by dividing the total score after treatment on total score before treatment. The effectiveness of the treatment is assessed by the magnitude of the coefficient: 1.2 and more or improvement of 1.0-1.19 points - no change, less than 1.0 - deterioration. This approach increases the objectivity of the assessment of treatment results and allows you to analyze changes in different groups of indicators and the impact of these changes on the overall result. The paper proposed to use four criteria to evaluate remedial effect.
The basic unit of the survey include: psychological tests (questionnaire sun, lüscher test, the test of Spilberger Hanina depression scale, the test Ravenna); psychomotor tests (black and red table, complex sensorimotor reaction); anthropometric measures; functional tests; assessment of biological age; heart rate variability. This APK is provided by the connection unit of analysis of heart rate variability via the USB port (Sakula AV, Tajibaev A.M., Amoscow A.V. the use of the automated system "Effect" for a comprehensive evaluation of the effectiveness of rehabilitation and restorative treatment in the clinic, proceedings of the II international Congress of Rehabilitation medicine and rehabilitation". - M., 2005, p.45-47).
It is not the key to these methods is they do not provide a quantitative assessment of the level of functional reserves of the organism; not provide for the issuance of targeted Wellness programs according to the survey results.
The closest to the technical nature of the present method is a method of assessing the functional state of the human body method, implemented in a hardware-software complex "Integrated indicator of health. It includes the study of adaptation and reserve capacity of the body, state of the cardiovascular, respiratory, Central nervous system, autonomic homeostasis, psycho-emotional status.
To assess the conditions and lifestyles of the program uses the following indicators: adequacy of supply; the mode of work and rest; physical activity; stress environment, including self-awareness in the society; bad habits.
The program "Integrated indicator of health (hereinafter: the Program) is intended for the quantitative determination of reserves of human health, based on objective findings. The Program is implemented, the principle of unity visceral and emotional areas of the body, a comprehensive approach to health assessment. Programme features view and save the results of the research allow dynamic control of the state of health analysis of the effects of environmental factors, the lifestyle of the person.
Diagnosis of reserves for individual health with the help of hardware-software complex "Integrated indicator of health is based on carrying out complex investigations: 1. assessment of nonspecific adaptive reserves of the body by the method of variation of cardiointervals with the mathematical analysis of heart rate (according to the method Bersenev et al.), 2. the rapid assessment of physical status (according to the method Globalarena), 3. evaluation of the functional state of the Central nervous system (CNS) according to the parameters of visuo-motor reaction (according to the method TDelegate); 4. determine the emotional state using a colorimetric test Luscher, 5. test-esteem, mental and physical state SAN (health-activity-mood).
The implementation of this standard set of tests is sufficient for a correct determination of the integral indicator of the health and its components. The first three tests are the block assessment of somatic health, and the last two relate to the psychological sphere.
The program also allows you to complete the survey assessment of physical performance (Harvard step test, the test PWC-170) in the absence of contraindications to submaximal load tests.
As a result of mathematical processing d is the R, obtained while performing individual tests. The program calculates the following key indicators:
- The rate of activity of regulatory systems (PARS), measured in points, characterizing the adaptive ability of the cardiovascular system;
- The index of the voltage (in arbitrary units), which is one of the important parameters in the evaluation of autonomic tone and used when calculating PARS;
- The level of physical capacity (in points);
- Functional reserves of the Central nervous system (%);
Levels of anxiety, emotional stability and ability to cope with stress;
- Indicators of well-being, activity, mood, and overall self-assessment (%).
These data in turn are subjected to mathematical treatment Program, resulting in a calculated (%) :
- Integral indicator (General reserves) health;
- Somatic component of health;
Psychological health component.
These indicators are not only numerically but also graphically (in the form of a cursor on the estimated semaphore scale, the position of which corresponds to the numeric value of the index and indicates its level).
All this information can be printed out and stored in computer memory (Sokolov A.V. Methodological recommendations "Integral assessment of reserve the individual health". - M.: 2003, p.12-14).
However, this method has a drawback as it is not presented algorithms for calculating the risk of development of somatic diseases and quantitative assessment; many of the indicators are directly or indirectly related to the amount of body weight, which indicates the dependence of results on weight. There is no formulation of a targeted Wellness programs and schemes in-depth survey.
The technical result of the proposed method is to obtain a quantitative integrated assessment of the functional reserves of the organism on the key functional systems, unification assess the level of functional reserves of the organism, the calculation of risk of development of common somatic diseases, the formation of a deployed conclusion with recommendations for improvement of persons at-risk groups, prevention of related diseases or in-depth examination.
This technical result is achieved by applying algorithms of processing of survey data, including consistent implementation of the following techniques: questionnaires, complaints and living conditions by touch clicking on the answers, the psychological and psychophysiological studies, anthropometric measurements, assessment of the level of glucose and cholesterol, the study of variability of ri is mA heart measurement of blood pressure and heart rate at rest and during exercise stress tests and then the results obtained lead in a single ten-point four-level scale, with recalculation of the integral indicators carried out by the formula:
where OS is the amount calculated using the formula. We have calculated the index belongs to the ten-point scale. V the upper border of the interval belonging to a ten-point scale. Z is the measured rate at the time of the survey. N is the lower bound of the interval, which is measured indicator. R is the difference between the upper and lower boundaries of the interval, which is measured indicator
then compute the total integral indicators that build schemes in-depth survey and quantify the risks of development of somatic diseases, through analysis of data obtained form the address Wellness programs.
The method is as follows.
The examination begins with a survey on the severity of complaints and conditions. The questionnaires were administered directly by the patient on the computer by touch clicking on the answers. The questionnaire severity of complaints consists of 28 questions (see Fig.1), which reflect the functional state of the organism and allow set up shop data is to some schema-depth survey. The survey on living conditions includes information about the nature of the work, lifestyle, physical activity, habits, existing diseases. After instruction and advanced training, conduct the following tests in the quality control responses using a computer mouse. Psychological research includes: a test of differential self-assessment of functional status (health, activity, mood) (see Fig.2a), the test of reactive anxiety of Spilberger-Hanina (see risb), Luscher colour test(see Resv). Psychophysiological study includes: simple sensorimotor reaction and response to a moving object. Then measure and put into a computer program weight, height, circumferences of waist and hips, these dynamometry hands and flexibility of the spine, blood pressure and heart rate at rest, the amount of lung capacity.
Next, perform a study of heart rate variability, with calculation of activity of regulatory systems Ramboesque. Studied should take a comfortable position in the chair, to relax, don't move and don't talk during the registration of the heart rhythm. Put the electrodes on the wrist: yellow - left hand, black and red on the right arm. Diagnostic module analyses the following data is variability: an index of tension of regulatory systems (INS), the activity indicator parasympathetic autonomic regulation (RMSSD), the standard deviation, as well as a comprehensive index of heart rate variability.
At the end of the survey conducted stress test Martin (with squats). Standing rest 1 minute, and then to determine the heart rate for 1 minute. Do 20 sit-UPS (hands up) for 30 seconds. Immediately after sitting down to determine the heart rate for 1 minute. At the end of the first minute of the rest pulse is measured and continues its measurement every 40 seconds to fully restore the original values. Remember the time at which the pulse was restored.
Before passing through the main part of the methodology, on an empty stomach is the Express-analysis of glucose and cholesterol using indicator test strips by means of photometry.
Elapsed time: 35-40 minutes.
The results of the tests lead to a single four-level integrated ten-point scale, which allows you to visually interpret the quantitative assessment of functional reserves.
Measured indices in the range up to 10 points, and are 4 levels of evaluation:
- poor condition - less than 2.5 points;
- satisfactory condition - from 2.5 to 4.99 points;
good status is s - from 5.0 to 7.49 points;
- excellent condition - from 7.5 to 10 points.
Scaling is done with consideration of the range of norms and deviations from it. Thus the value of the median is equal to 10 points, and the extreme values of the interval norms correspond to 2.5 points. The deviation from the median 16,66% corresponds to 7.5 points, and 5 points corresponds to 33.3% deviation from the median.
The conversion formula from values measured values to a ten-point scale integrated assessment of functional reserves of the organism is as follows:
where OS is the amount calculated using the formula. The calculated parameter is already a ten-point scale. V the upper border of the interval, which is measured parameter belonging to a ten-point scale. Z is the measured value at the time of the survey. N is the lower bound of the interval in which the measured parameter. R is the difference between the upper and lower bounds of the interval in which the measured parameter.
Consider the principle of scaling on the example of the calculation of blood pressure. Consider the example of applying the algorithm for the calculation of the level of blood pressure. Limit of normal values of systolic blood pressure ranged from 114 mm Hg to 130 mm Hg Calculated the median, which will correspond to 10 points is: Median=(114+130)/2=122, accordingly, 122 mm Hg=10 points in the integral scale. From the median postponed on 16,66%, and the resulting boundary 119 mm Hg and 125 mm Hg, corresponding to 7.5 points, and the interval of the high level is as follows: (119 - 122 - 125) in the measured indicators (7,5 - 10 - 7,5) - in a ten-point scale. Further from the median postpone 33,3%, the resulting boundary 117 127 mm Hg and mm Hg, corresponding to 5 points. The deviation from the median in 49,89% is equal to the norm and corresponds to 2.5 points. Indicators not included in the above intervals correspond to the interval from 0 to 2.49 points.
Thanks to the unification of research results into a single integral scale, you can calculate the total integral indicators for different number of values.
For two variables:
where a and b are integrable indicators
a and b are weighting coefficients, is inversely proportional to the metrics
Inline this coefficient (a) in the formula:
For four variables, the formula looks as follows:
For five variables, the formula looks as follows:
The obtained formulas allow us to calculate the total integral is an indicator of the level of functional reserves. This has resulted in the following General integral indicators: the integrated indicator of self-health, an indicator of the functional state of the cardiovascular system, the indicator of the physical condition, biochemical status, and mental status.
The integral indicator self-assessment of health (IPSS)=2*PSZ (indicator self-assessment of health based on responses to the questionnaire severity of complaints)* Health (index of well-being test SAN)/(PSZ+Health).
The integral indicator of the functional state of the cardiovascular system (IPSS)=3*HR*Adest"*Diast/(Adsit*Addict+HR*Adest"+HR*Atlast).
The integral indicator of the physical condition (IPPS)=2*D (Dynamometry hands) * SE (Flexibility of the spine)/(D+SE).
The integral indicator of the biochemical status (IPBS)=2*G(glucose)*X(cholesterol)/(G+X).
The integral indicator of the psychological condition (thirty-two)=2*N (sentiment index test SAN)*RT(a measure of reactive anxiety test Spilberger Hanina)/(H+RT).
Thanks to the calculated total integrated indicators, it is possible to produce a differentiated assessment of the functional status of the organism and grant schemes in-depth survey.
Patient R., born in 1985 entered the "RRC VM and with complaints is on unpleasant sensations in the stomach, pain. History: duodenal ulcer.
Independently of the subject fill in the registration data on the computer. Next, the subject is undergoing questioning by the severity of the complaints, conditions of life, and psychological and psychophysiological research. Conducted a study of heart rate variability and other techniques.
Next, the nurse records the following data research system: weight, height, circumferences of waist and hips, these dynamometry hands and flexibility of the spine, blood pressure, heart rate, vital capacity (VC).
The survey yielded the following results (see table 1). The table presents the values obtained in the survey, and their conversion into a ten-point scale.
|The survey results.|
|No.||The parameter obtained as a result of research||Values||Values in 10 point scale|
|1||Setting self-assessment of health||4 srvc. units||5|
|2||Assessment of heart rate (HR)||66 beats./min||from KZT9, 573|
|3||Setting activity of the parasympathetic autonomic regulation (RMSSD)||45 MS||8,922|
|4||Index of tension of regulatory systems (INS)||105 UEL. units||9,285|
|5||The standard deviation (SDNN)||44 MS.||6|
|6||Glucose level||4.7 mmol/l||9,375|
|8||Assessment of arterial pressure|
|8.1.||HELL systolic||123 mm Hg||9,16|
|8.2.||HELL diastolic||83 mm Hg||3,75|
|9||The body mass index (Quetelet index)||25,03 srvc. units||6,475|
|10||The result of the test on the flexibility of the spine||4 mm||9|
|11||These dynamometry||56 Dean||10|
|12 Psychological research|
|At differential self-assessment of functional status (SAN)|
|12.2.||The scale of reactive and personal anxiety of Spilberger Hanina. The level of reactive anxiety.||29||8,5|
|13 Psychophysiological research|
|13.1.||Performance test of a Simple sensorimotor reaction"||214||10|
|13.2.||Performance test Reaction to a moving object||90||9,107|
Consider the example of the presentation of the results in a ten-point scale.
In the study received the amount of blood pressure systolic equal to 123 mm Hg, this value lies within the range from 122 mm Hg to 125 mm Hg Using the conversion formula, we get the value in a ten-point scale:
where 9,16 - calculated parameter belonging to a ten-point scale. 7.5 to the lower boundary of the interval in a scale. 123 - obtained parameter during the examination. 125 - the lower bound of the interval in the scale of the measured parameter. (-3) - the difference between the upper and lower bounds of the interval in which the search is measured by the parameter, that is, R=122-125=-3.
The obtained integral indicator belongs to a ten-point scale. Thus, the conversion formula allows you to convert any quantitative characteristic in a single integrated four-level ten-point scale.
Similarly calculate the parameter of diastolic blood pressure and heart rate. Blood pressure diastolic equal to 83 mm Hg, this value lies within the range from 82 to 84 mm Hg mm Hg Using the conversion formula, we get the value in a ten-point scale:
where 3,75 - calculated parameter belonging to a ten-point scale. 2,5 - start interval in a scale. 83-obtained parameter during the examination. 84 - the lower bound of the interval in the scale of the measured parameters. (-2) - the difference between the upper and lower bounds of the interval in which the measured parameter, that is, R=82-84=-2. The indicator belongs to a ten-point scale.
Indicator heart rate equal to 66 beats. min, this value lies within the range from 61,4 beats. min to 72.6 beats. min Using a conversion formula, we get the value in a ten-point scale:
where 8,97 - calculated indicator belonging to a ten-point scale. 7.5 to the lower boundary of the interval in the decade, illinoi scale. 66-received indicator during the examination. 72,6 - the lower bound of the interval in the scale of the measured parameters. (-11,2) - the difference between the upper and lower bounds of the interval in which the measured rate, i.e. R=61,4-72,6=-11,2. The obtained integral indicator belongs to a ten-point scale.
The obtained integral indicators further used in the calculation of the total integral indicator of the functional state of the cardiovascular system (IPSS).
The resulting index ranges from 5 to 7.5, which indicates "good" functional state. Similarly, the calculated remaining overall performance.
The integral indicator self-assessment of health (IPSS)=2*MTS*Health/(PSZ+Health)=2*5*9,375/(5+9,375)=6,52.
The integral indicator of the physical condition (IPPS)=2*L*SE/(D+SE)=2*10*9/(10+9)=9,47.
The integral indicator of the biochemical status (IPBS)=2*G*X/(G+X)=2*9,375*8,639/(9,375+8,639)=8,99.
The integral indicator of the psychological condition (thirty-two)=2*N*RT/(N+RT)=2*7,85*8,5/(8,5+7,85)=8,16
The obtained integral indicators help to assess the level of functional reserve of the patient. As can be seen, the results are within the level of "above average", which indicates "well the level of health.
The proposed method of assessing the functional state of human organism was applied at 180 patients with different pathologies, as well as healthy individuals in the amount of 110 people. Age range from 15 to 75 years. Studies were conducted on the basis of Federal state institution "Russian research center BM and MOH", clinics FGOU VPO "Russian state" and the fitness centre "Energy fit".
Analysis of the results of the conducted research allows to quantify the functional reserves in a single integrated four-level scale, to give the scheme a thorough medical examination. The General integral numbers provide integral and differential quantification of the level of functional reserves. Integrally presents data allow in the future to assess the dynamics of the patient's condition.
The method of assessing the functional state of human organism, including the estimation of parameters characterizing the state of the body, the definition of the integral indicators of the functional state of the organism, characterized in that the estimate of the parameters characterizing the functional state of the cardiovascular system, physical condition, biochemical status, psychological status, and self-reported health, the value of each parameter, the corresponding norm is transferred in a single decade, Ballou four-level scale,
why in the range of normal values determine the median, taking her for 10 points, from the median lay Sigma, corresponding 16,66%, 33,3%, 49,89%, caliroa thus, normal values of the estimated parameter, corresponding to 7.5, 5, 2.5 points, then the individual values of each parameter transferred in a ten-point scale using the formula:
where V is the lower bound of the interval belonging to a ten-point scale, which is measured parameter, Z is the individual value, parameter, N is the lower bound of the interval in which the individual is the value of the parameter, R is the difference between the upper and lower boundaries of the interval, which is measured by the indicator, on the basis of obtained for each individual parameter values OS, define the integral indicator (I): self-assessment of health and functional status of the cardiovascular system, physical condition, biochemical status, psychological state, which, depending on the number of parameters characterizing the integral indicator, use one of the formulas of definition Inwhere n is the number of parameters: a, b, C, D, E options:
I5=5·A·B·C·D·E/(A·B·C·D+A·C·D·E+A·D·B·E+A·E·A·C+B·C·D·E), received a ten-point scale values of integral parameters characterizing the functional state of the human body: self-reported health, functional status of the cardiovascular system, physical condition, biochemical status, psychological state, compared with the intervals: "less than 2.5 points, from 2.5 to 4.99 points, from 5.0 to 7,49 points, from 7.5 to 10 points," making the conclusion of unsatisfactory, satisfactory, good or excellent condition.
SUBSTANCE: invention relates to field of medicine, namely to oncology. To diagnose cancer and potential resistance of malignant cells to hypoxia, prepared is cell imprint or suspension of cells, for which conditions of hypoxia are created and spectro-flowmetric analysis of said cells in dynamics of development of process of photodestruction of NAD(P)H fluorescence is performed. Conclusion about presence of tumour cells and their potential resistance to hypoxia is made by increase of NAD(P)H fluorescence intensity in conditions of darkness. Conditions of hypoxia are created by placement of cell suspension or cell imprint between two glasses for cytologic analysis. Spectro-flowmetric analysis is carried out in the range 440-470 nm, with length of excitation wave 365-370 nm.
EFFECT: method makes it possible to diagnose cancer and potential resistance of malignant cells to hypoxia.
3 cl, 6 dwg, 1 ex
SUBSTANCE: invention relates to field of sports medicine, namely to methods of laboratory diagnostics. In order to carry out laboratory diagnostics of physical activity level on organism of badminton player athlete, content of calcium and phosphorus in mouth fluid before and after physical activity is determined. If content of calcium and phosphorus before and after physical activity changes by value exceeding 9.0 and 42.9% respectively, high level of physical activity is determined; by value lower than 4.6 and 22.5 respectively, optimal level of physical activity is determined.
EFFECT: method makes it possible to carry out non-invasive laboratory diagnostics of physical activity level on organism of badminton player athlete.
1 dwg, 5 tbl
SUBSTANCE: invention relates to field of medicine, namely to pediatrics. In order to diagnose biliary tract dysfunction in children acetic and propionic acids are determined in blood and saliva by gas-chromatographic method. If concentration of any of two acids in blood: acetic in the range more than 0.3264 to 1.1000 mmol/l, propionic in the range more than 0.0274 to 0.0990 mmol/l and/or if concentration of any of two acids in saliva: acetic in the range more than 0.0683 to 0.5900 mmol/l, propionic in the range more than 0.0066 to 0.0430 mmol/l, biliary tract dysfunction is diagnosed.
EFFECT: method makes it possible to diagnose biliary tract dysfunction in children.
3 ex, 3 tbl
SUBSTANCE: invention relates to medicine, in particular to dentistry and is intended for estimation of esthetic results of dental treatment. Essential esthetic face characteristics: symmetry of features (S), state of teeth in esthetically important zone (ST), naturalness of mimics (M) and smile (S) are estimated before and after treatment by formula: Ie=(S+ST+M+S):4, where Ie is index of total esthetic result of dental treatment. Estimation in points of each face characteristics is performed on the basis of bilateral contact expertise by scheme "doctor-patient". Quantity of points of each essential face characteristics - feature symmetry, state of teeth in esthetically important zone of oral cavity, mimics naturalness, smile naturalness is determined on the basis of reduction of possible highest mark in 100 points, where one point corresponds to one percent. Total esthetic result is estimated as good, when Ie equals 95-100 points, as satisfactory - from 90 to 94 points and Ie is considered to be unsatisfactory if Ie is less than 90 points.
EFFECT: method makes it possible to increase accuracy of estimation of dental treatment results due to bilateral contact expertise by scheme "doctor-patient" and system of calculation of value of estimation of each essential face characteristics.
SUBSTANCE: invention relates to field of medicine, namely to traumatology and orthopedics. In order to estimate intensity of destructive processes in case of knee joint traumatic injury and prediction of development of post-traumatic arthrosis, content of oxyprolene in determined in rats after modelling of knee joint trauma. Content of protein-bounded oxyprolene in plasma higher than 75 mcmol/l with simultaneous increase of free fraction of oxyprolene higher than 16 mcmol/l testified to active reparation at the background of continuing intensive destruction of collagen structures resulting in deforming osteoarthrosis.
EFFECT: method increases accuracy of estimation of destructive processes intensity in case of traumatic injury of knee joint and prediction of development of post-traumatic arthrosis due to determination of quantitative and fractional content of oxyprolene.
SUBSTANCE: invention relates to medicine and can be used for examination and diagnostics of biological object or its part. Method of examination and diagnostics includes the following stages: image of biological object is obtained, obtained image is calibrated and standardised, computed morphodensitometric analysis is performed. For this purpose zones of interest are identified on obtained image, required ranges of charcteristics of signal intensity are determined by fixed distribution of characteristics of irradiation interaction with substance on image, graphic presentation of distribution of intensities of image elements is formed with possibility of superposition of graphic presentation on image. After that, morphometric and densitometric parameters of image are determined, said parameters of examined object are compared with analogous parameters of reference group and decision about condition of examined object is taken on the basis of comparison results. When image of biological object is obtained, shooting of examined object is performed with additional application of illuminator with changeable emission spectrum. Source of illumination is moved on circumference around object of examination and angle of ray incidence is changed discretely at each following circle due to movement of illuminator, shooting is performed and series of object images in different spectrums of illumination for each position of illumination source is obtained, calibration of light intensity is performed in different spectrums of illumination. After that, several pictures of image are accumulated with further obtaining by said pictures average image for each version of illuminator spectrum separately, graphic presentation of distribution of intensities of image elements is performed in form of histogram. Histogram is performed with possibility of linearise and expanding in automatic, interactive modes.
EFFECT: application of claimed invention will make it possible to increase accuracy and hardness of obtained images of examined objects, and due to it obtain more objective picture of changes in biological object at the level of its structure.
SUBSTANCE: invention relates to field of medicine, namely to phthisiology. In order to carry out differential diagnostics X-ray examination of patient's lungs and laboratory test of blood parameters are performed. In case if inhomogeneous and highly-intensive infiltration of lung tissue is detected, level of cortisol in blood serum is additionally determined, and if its value is from 808 nmol/l to 2500 nmol/l, caseous pneumonia is diagnosed, if value is from 159 nmol/l to 718 nmol/l, infiltrative tuberculosis of lungs is diagnosed.
EFFECT: method increases accuracy of differential diagnostics of caseous pneumonia due to determination of level of cortisol in blood serum.
SUBSTANCE: stage of atopic dermatitis in children is diagnosed by immunobiological assay of patient's peripheral blood. A degree of lymphocyte expression of CD95+ markers is evaluated. If the CD95+ value exceeds 60.09% enables diagnosing an acute stage of the disease, while the CD95+ values 20.33% to 60.09% shows a chronic stage.
EFFECT: well-timed prediction of the clinical course ensured by accuracy and acceleration of dianosis of transmission of the acute stage of atopic dermatitis in children into the chronic stage.
1 tbl, 2 ex
SUBSTANCE: invention refers to medicine, namely endocrinology and diabetology. The clinical course of diabetes mellitus is predicted by evaluating the following risk factors: tongue rolling, myopia, jug ears, joint hypermobility, platypodia, astenic body, diagonal fold of the earlobe, high skin elasticity, varicosity, visceroptosia, height/span > 1.1, clinodactyly, mitral valve prolapse, renal cysts, Gothic palate, chest distortion, biliary tract deformation, arachnodactylia, false left-ventricular chordas. If the patients suffering type 1 diabetes mellitus shows 5 or more indicators, a high risk of developing diabetic nephropathy and arterial hypertension is predicted. If the patients suffering type 2 diabetes mellitus shows 5 or more indicators, a high risk of developing ischemic cardiac disease is predicted. If observing 4 or less indicators in the patients suffering types 1 and 2 diabetes mellitus, a minimum risk of the unfavourable clinical course of diabetes mellitus is predicted.
EFFECT: method enables predicting the clinical course of diabetes mellitus in the patients with types 1 and 2 diabetes mellitus on the basis of the indicators of increased dysplastic stigmation.
SUBSTANCE: invention relates to field of medicine, in particular to pediatrics. When child is one month old, their somatic and neurological status is estimated and equation of regression is calculated: 42.423-4.373 ×No of group - 0.524 × sex - 0.972 × retardation of development of motor functions - 1.264 × intrauterine infection - 0.763 × allergic dermatitis- 1.212 × disembriogenesis stigmas + 1.807 × advancing development of communicative functions, where - No of group - 2 for group of children, who receive and No of group 3 for group of children who do not receive additional medical-corrective procedures; sex - 1 female, 2 - male; retardation of development of motor functions - 0 no, 1 present; intrauterine infection - 0 no, 1 present; allergic dermatitis - 0 no, 1 present; disembriogenesis stigmas - 0 no, 1 present; advancing development in communicative functions - 0 no, 1 present. Equation is calculated twice - for situation of application and absence of carrying out complex of medical-corrective procedures. Obtained values for both groups are compared and necessity of carrying out medical-psychological-pedagogical programme is determined.
EFFECT: method makes it possible to obtain objective estimation of age development of child and determine necessity of carrying out correction of their development.
FIELD: medicine, clinical toxicology.
SUBSTANCE: at patient's hospitalization one should gather the data of clinical and laboratory values: on the type of chemical substance, patient's age, data of clinical survey and laboratory values: body temperature, the presence or absence of dysphonia, oliguria being below 30 ml/h, hemoglobinuria, erythrocytic hemolysis, exotoxic shock, glucose level in blood, fibrinogen and creatinine concentration in blood serum, general bilirubin, prothrombin index (PTI), Ph-plasma, the state of blood clotting system. The state of every sign should be evaluated in points to be then summed up and at exceeding the sum of points being above "+20" one should predict unfavorable result. At the sum of "-13" prediction should be stated upon as favorable and at "-13" up to "+20" - prediction is considered to be doubtful.
EFFECT: higher accuracy of prediction.
2 ex, 3 tbl
SUBSTANCE: method involves studying blood samples with venous blood mixed with vital stain like methylene blue. Degree of vital stain absorption by erythrocytes is determined by applying photocolorimetry. The value drop being more than 25%, extracorporal detoxication is to be predicted as ineffective.
EFFECT: simplified method.
SUBSTANCE: method involves evaluating prognostic signs and calculating their prognostic weight. Consciousness disorders and diagnosis set at prehospital stage are used as the prognostic signs. Calculations are carried out from formula: lethal outcome %= -15.99+19.1•C+14.7•DS-2.79•C•DS, where C is the ranged consciousness disorder degree, DS is the diagnosis set prehospital stage. The higher is the value, the higher is lethal outcome probability.
EFFECT: accelerated and simplified prognosis method.
SUBSTANCE: method involves evaluating proliferating processes by calculating index of positive cell nuclei (Ki-67). The Ki-67 value being from 6 to 16%, erosive ulcerating stomach lesions accompanied by stomach hemorrhage and hemorrhagic shock is to be predicted. The value being from 17 to 30%, erosive ulcerating stomach lesions without hemorrhage is to be predicted.
EFFECT: high accuracy of prognosis.
FIELD: medicine, urology.
SUBSTANCE: the present innovation deals with differential diagnostics of prostatic cancer and other prostatic diseases at the stage of primary inspection. The method includes the detection of PCA and calculation of probability coefficient for prostatic cancer (PCC) by the following formula: where e - the foundation of natural logarithm (e=2.718…), PCA - the level of total blood PCA in ng/ml, V - patient's age in years. At PCC value being above 0.2 one should diagnose prostatic cancer and to establish final diagnosis one should perform polyfocal prostatic biopsy. The method enables to increase accuracy of diagnostics at decreased number of unjustified prostatic biopsies.
EFFECT: higher efficiency of diagnostics.
SUBSTANCE: method involves determining homeostasis values and absolute number of neutrophils and lymphocytes in particular. Low neutrophil number being determined in a child during a number of years and low number of lymphocytes in the persons from the nearest child surrounding, the persons are to be examined for given psychosomatic pathology availability with following medical treatment to be applied to eliminate the detected pathology and to normalize homeostasis values and lymphocyte number among others.
EFFECT: enhanced effectiveness of prophylaxis.
SUBSTANCE: method involves measuring radio signals and low frequency spectra of signals emitted by oral cavity organs and tissues in maxillofacial area. Decoding and comparison of the spectra to healthy organs and tissues spectra is carried out. Then, modulated information signals in infra-low frequency bandwidth corresponding to healthy organs and tissues signals are applied. Low frequency radio signals and spectra are measured. Treatment with modulated signals in infra-low frequency bandwidth is applied directly to organ or its location zone via skin.
EFFECT: enhanced effectiveness in diagnosing and treating oral cavity organs and tissues in maxillofacial area.
5 cl, 3 tbl
FIELD: medical engineering.
SUBSTANCE: device has bottom and cover joined in inseparable way so that no chance is available to mismatch covers belonging to different containers. The bottom and internal part of cover are lined with sponge so that the allergenic disks are kept in uniformly wetted state. Grate with holes is attached to lower sponge, the holes narrowing downward as cones so that the allergenic disks are kept in stable vertical position.
EFFECT: simplified access to disks using forceps; excluded misidentification risk.
5 cl, 6 dwg
FIELD: medicine, operative gynecology.
SUBSTANCE: at final stage of laparoscopic operation for 5-7 min one should introduce 16 U lidase in 1 ml 2%-lidocaine solution into uterine mesentery from both sides, and then, by not removing a needle - a half of single dose of antimicrobial preparation in 1 ml 2%-lidocaine solution, then in postoperational period - an antimicrobial preparation applied during laparoscopy lymphotropically under mucosa of lateral vaginal arch from both sides for 5-7 d once daily and one antimicrobial preparation - intravenously for 5-7 d, moreover, as antimicrobial preparations one should apply gentamicin, metrogyl and other preparations permitted for intravenous application. The present innovation stimulates lymphatic drainage in area of inflammation and activates interstitial humoral transport of antimicrobial preparations that, in its turn, favors complete sanitation of inflammation foci and prophylaxis of disease relapses.
EFFECT: higher efficiency of therapy.
1 cl, 1 ex
SUBSTANCE: method involves applying biological indicator as mixture composed of 0.1% aqueous solution of amino acids: leucine, glycine, proline, serine, phenylalanine, histidine, oxyproline, arginine, glutamic amino acid and aspartic amino acid, 0.5% aqueous solution of neuromediators like dopamine and histamine, 12% aqueous solution of magnesium sulfate in proportion of amino acids : neuromediators : magnesium sulfate = 4:1:5. Indicator kept on neck surface in thyroid gland projection during 3-5min. It is dried at T=+35-40°C, studied in polarized light with quartz compensator. Columnar, columnar-and-striated, sheaf-like crystals and discharged polygonal variegated chambers being observed, thyroid gland hyperfunction is to be diagnosed.
EFFECT: high accuracy of diagnosis.