Life-time diagnostic technique for zoonosis trichocephalus trichiurus and trichocephalus suis by egg microstructure

FIELD: medicine.

SUBSTANCE: technique involves whipworm egg sampling on a slide, clearing in the mixture of 98.5% glycerol of density 1.256 and 40% lactic acid taken in proportions 1:1 then the eggs are covered with a cover slip and examined under the microscope at magnifications 600, 1200 and 900 under immersion. The microstructural differences of eggs are determined, and whipworms are differentiated by the following signs: Trichocephalus trichiurus have high, thin-neck and wide-cap plugs on sharp-pointed egg poles; the 1-st external transparent cover encloses the whole egg and is visible close to the poles in the form of a transparent disk, or on the plug caps; the plug necks are helically-formed and has 4 rings; the 3-rd ring counting from an apical ring is dome-shaped thereby differing from the previous 2 rings; the 4-th ring can be transparent, or keratised: function as a linker between a base of the plug neck and the 4-th embryo shell; there are spines visible on the 2-nd keratised shell; the egg poles comprise: platelike spines of various configurations; Trichocephalus suis has short-neck and cap plugs not projecting from an egg surface; on the egg poles, the 1-st transparent shell is visible in the form of a fountain of transparent microstructures extending from the eggs; the helically-formed plug neck with 4 rings; the 4-th ring is dome-shaped and fastens the base of the plug neck to an internal embryo shell; it has: spines on the 2-nd shell, platelike spines and a stylette on the plugs.

EFFECT: technique provides the life-time differentiation of whipworms to types: Trichocephalus trichiums and Trichocephalus suis zoonosis by egg microstructure.

1 ex, 10 dwg

 

The invention relates to the field of veterinary medicine, and more specifically to Parasitology (helminthology) for the production lifetime of the differential diagnosis to species of helminths on the microstructure of eggs: zoonotic Trichocephalus (Trichuris) trichiurus (affecting humans, monkeys and larval rearing of domestic and wild pigs) and Trichocephalus (Trichuris) suis (affecting pigs, wild boars=wild boar in the larval stage of a person), and is intended for use in diagnosis methods chaprovskoye (flotation, sedimentation, combined) and helminthological studies of environmental objects (on livestock farms, circuses, zoos, in vivaria institutions), in which the diagnosis of a disease to kind of helminth if not known parasite host: human, monkey, pig.

Famous works of Cerebral A.A. (1967) (3), which through complex manipulations on eggs Trichocephalus suis: strong acetic acid and strong sulphuric acid (10-15 hours), revealed 4 shell the eggs of this species (SS-425), and Dinnik N.N., which dimension 200 eggs "human" and 200 pork vlasoglavy revealed slight differences in the sizes of eggs Trichocephalus trichiurus (L., 1771) and Trichocephalus suis Schrank, 1788.

Works listed above have their scientific value, however, have some disadvantages in scientific and practical terms.

4 shell, the detection is by Brain A.A. (3) eggs Trichocephalus suis were identified by the author is very complicated and time consuming experiments, and it is a difficult diagnosis to Trihotel, but in practical applications, especially for the detection of contamination of the external environment (soil, grass, fodder, bird feeders) on livestock farms, svinopapka, in circuses, zoos, vivaria institutions veterinary and medical profile, biofactories, private pig farms and from private citizens, which contain not only a mini-pigs, but ordinary pigs and monkeys, or in zoos belonging to different film companies, very difficult to diagnose eggs striking mini-pigs, Pets, circus, zoo pigs and wild pigs species Trichocephalus suis and especially differential diagnosis with eggs of the helminth damaging human and monkey species Trichocephalus trichiurus proposed Brain A.A. (3), as it requires a lot of time and chemicals.

This is not only scientific, but also practical, as the external environment circuses, zoos, farms and locations of the film may be contaminated (dirty) discharge not only from pigs, but also from the people, trained monkeys, as trichocephalosis at this time can be observed not only by local residents but also visitors: tourists, foreign workers from Turkey, Africa, the middle is Asia, in the South of Russia, Moldova, Ukraine), students from Asia and Africa, South and Central America, Australia and imported pigs from other countries.

Dinnik NN. (1941) (1) revealed a difference between the average sizes of these two types of trichocera Trichocephalus suis (from pigs) and Trichocephalus trichiurus (view from humans, monkeys), but this characteristic is the size of the eggs, has more scientific value than practical, because in practical terms in the study of contamination of the external environment (shooting sites, sex from private owners, zoos, circuses) to collect and measure of the investigated samples 400 or more eggs of trichocera is hardly consistent with reality, as practitioners have to deal often with other invasions, also require time and sometimes even other methods.

These distinctive features eggs .suis and .trichiurus has proven to be extremely insufficient for the diagnosis and especially for the differential diagnosis of the species, the more that the study of the external environment (soil and premises in circuses, zoos, shooting platforms, flats and private owners of animals) can be and eggs pathogens other diseases: whipworms from ruminants and carnivores (dogs, cats, foxes, ferrets), capillaria, hepatically, diktofoni.

Famous works of beekeeper V.E., Pasechnik V.E.(4, 5, 6, 7, 9), in which the author first in the world of science (with the accordance with the scientific literature) gives the data for the study of their microstructure and ultrastructure of the eggs 3 types of trichocera from ruminant animals under light and scanning electron microscopy and the identification of them with the most the characteristic features of the ultrastructure of the eggs.

Known patent No. 2396069 (application 2008140245/13, 13.10.2008) beekeeper V.E. (8) "Method in vivo differential diagnosis of trichocera Trichocephalus ovis, Trichocephalus skrjabini and Trichocephalus capreoli from ruminants", developed by the author based on the study of them for the first time in the scientific world (according to the scientific literature) under a light microscope: the microstructure of the membranes, samples and cervical samples of eggs from 3 types of trichocera.

These works beekeeper V.E., Pasechnik V.E.(4, 5, 6, 7, 8, 9) have a great scientific value in relation to fundamental research: morphology, systematics and practical value: improvement of in vivo diagnosis and therapy in trichocephalosis zoo, circus, tribal, and rare species of ruminants.

However, the scientific work of the beekeeper V.E., Pasechnik V.E.(4, 5, 6, 7, 8, 9) differential characteristics on the microstructure and ultrastructure of the eggs Trihotel, which is extremely important when developing a differential post-mortem and in vivo diagnostics, refer only to trichocephalus agricultural and wild ruminants, and as for in vivo differential diagnosis microstructure eggs Trihotel from pigs, primates and humans, this question remains open.

In addition, Brain A.A. (3) studied only the structure of the membranes (by the false chemical manipulation of eggs Trichocephalus suis), however, the microstructure of the samples and cervical samples of eggs Trihotel type Trichocephalus suis was not understood.

In vivo infection of domestic and wild pigs, humans and monkeys with trichocephalus can be installed awesomechickin method of investigation of faeces (kopirovskaja), because their eggs are fairly typical of the kind of Trichocephalus Schrank, 1788, the typical shape of a barrel with two samples at the poles, and diagnosis taking into account that the studied feces samples were individually collected from a particular animal species: pigs, monkeys and humans.

However, the difficulties in diagnosis arise when the need to clarify the diagnosis up to species of parasite: Trichocephalus trichiurus, Trichocephalus suis, when contamination of the external environment and premises in zoos, circuses, breeding facilities and private owners (the external environment is often contaminated with eggs of the pathogen as from man, and from monkeys and pigs!), as features of the microstructure of the eggs of certain species Trihotel poorly understood. This is necessary for scientific purposes: the improvement of the taxonomy of worms and with a practical purpose: in the study of contamination of the external environment: sex, water, food, navodorozhivanie and Refine the effectiveness of deworming in the premises and exercise sites vivaria, zoos and circuses.

The value of the CI Trichocephalus (Trichuris) trichiurus 0,050-0,065 mm 0,022-0,030 mm and the average 0,0570,026 mm, a Trichocephalus suis 0,057-0,0670,025-0,0355 mm and the average 0,0620.029 mm, that is, to detect a real difference in their size (which is very minor) researchers (scientific and especially with a practical purpose) you must measure not less than 200 eggs of parasites from each of the above types of trichocera that for scientific purposes is acceptable, but in practice this is unrealistic.

Therefore, the search and development of in vivo diagnostic methods on the microstructure eggs Trihotel from primates, humans and pigs is an important task of the veterinary and medical science.

Therefore, all researchers, no matter how well-known the in vivo method is not used (2): flotation on Fulleborn and its modification, sedimentation) on Ehrlich and its modifications, combined the Darling and its modifications, diagnose illness by type of animal (considering that species of animal is also the form of the parasite): at home, circus, zoo and wild pigs - Trichocephalus suis and humans and monkeys - Trichocephalus trichiurus, and in the study of the external environment (contaminated with eggs of different species) differential diagnosis Trihotel to no, because the way lifetime diagnostic Trihotel to Trichocephalus trichiurus and Trichocephalus suis has not been developed, except 3 species of mesoglea from domestic and wild ruminants, defined and differentiae is haunted by the microstructure of the eggs according to the method of the beekeeper VE (8 - patent No. 2396069).

In domestic and wild pigs and humans are parasites of various kinds Trihotel, however, in the study of contaminated external environment: soil on the playgrounds, on the set of film companies in the areas of circuses, zoos and vivaria of biofactories, research institutes, in samples can be detected (and usually find!) eggs not only Trihotel from swine, human, and eggs Trihotel from dogs, cats, ferrets, foxes, domestic and wild (trained) ruminants.

Known methods of in vivo diagnostics: flotation on Fulleborn with a solution of sodium chloride (1920) and its modifications (2); sedimentation method according to Ehrlich (1927) and its modifications (2); method combined by Darling (1911) (sedimentation and flotation) and its modifications (2)that in the study on trichocephalus domestic and wild pigs, monkeys and humans have one drawback: they allow the researcher lifetime supply General the generic diagnosis of the disease trichocephalosis. Therefore, for scientific purposes and in practical terms it is necessary to specify the diagnosis of disease caused by worms up to view.

This usually happens either posthumously: when helminthological studies of animals and humans, or after degelmintizatsii if you can actually find imaginal f is RMI parasites what in practical terms is not always possible, as you need every day to collect and analyse the maximum number of faeces from pigs, monkeys and humans (up to a possible detection of Mature worms!).

This is especially important when the disease:

high-value breeding and trained pigs and monkeys used in circus performances, household purposes;

pigs and monkeys that are the subject of a laboratory model for the study of especially dangerous infectious and parasitic zoonotic diseases of humans, farm and wild animals, circus "performers";

valuable hunting species of animals: boar=boar;

breeds of pigs, mini-pigs, domestic pigs, which are suppliers not only aesthetic pleasure - trained "artists" circuses: pigs and mini-pigs - but also suppliers of valuable products for the physical needs of man (bacon meat, lard, high-quality leather products, gloves, boots, jackets, etc.);

when disease and death of endangered species of apes: orangutans (1 orangutan reaches from 25000 to $ 50000), and given the indispensability of monkeys in the study, treatment and vaccine especially dangerous viral, bacterial and parasitic diseases of man becomes more ponat the urgency of improving diagnosis of parasitic diseases, in which to clarify the diagnosis to Trihotel Trichocephalus trichiurus; Trichocephalus suis - pathogen should be investigated postmortem (autopsy), which leads to huge economic losses and sometimes can cause the death of not only animals, but also disability most valuable for our country being the man himself, or rather our children (disease trihotsefaleza children registered in all countries of the world and even in those where economic and social conditions are at the highest level: France, Italy, Canada, UK, USA).

Sometimes reducing the efficacy of the methods of prevention and treatment, as there is no in vivo method of differential diagnosis to species of pathogens Trihotel from primates, humans and breeding, circus, zoo pig Trichocephalus (Trichuris) trichiurus, Trichuris suis in contamination of the external environment sources of disease and man and monkeys, and domestic and wild pigs in space for animals and human premises: apartments, houses, garden houses and cottages, or the beaches, river, sea, etc.

The purpose of the invention. The aim of the invention is the use we studied for the first time microstructure of membranes, cervical samples and Mature embryo 500 eggs Trihotel extracted from the vagina of females each identifitsirovana is about the species of the parasite, which were collected by the method of the helminthological autopsies from pigs from Moldova and the Russian Federation and from degelmintizirovano shepherds and shepherds in Moldova, spontaneously infected with trihotsefaleza, and which could be applied to improve the efficiency of the method and diagnosis not only kind, but also to Trihotel microstructure eggs: Trichicephalus (=Trichuris) and trichiurus Trichocephalus (=Trichuris) suis - pathogens monkeys, humans and pigs in the study of contaminated external environment eggs pathogens parasitic zoonoses (helminthosis).

1. This goal is achieved by the fact that when performing diagnostics on trichocephalosis by contamination of the external environment: coastal, river, lake beaches, households, farmers, HOMESTEAD plots, flats citizens (with monkeys, pigs, mini-pigs), arenas and facilities circuses, zoos, water from the troughs, feed and care for the animals, vivarium institutions, stages of film production companies (2), apply the developed method in vivo differential diagnosis of zoonotic Trichocephalus trichiurus and Trichocephalus suis microstructure eggs from monkeys, humans and domestic and wild pigs, which is in the selection on a glass slide eggs, their enlightenment in the glycerin solution plus 40% lactic acid in a ratio of 1:1, cover glass slide and viewed under a microscope at magnification: 600, 1200 and 900 under immersion, different from the known methods (2) in vivo diagnostics (flotation, sedimentation, combined that with the aim of improving the efficiency of diagnosis carried out the determination of microstructural differences eggs, putting the diagnosis of the disease not by type of parasite host (pigs, monkeys, humans), and the characteristics of the microstructure (identified by us for the first time!) each species of parasite eggs (especially when environmental pollution eggs pathogens invasion to determine what species of animal discovered invasive beginning, no way).

Trichocephalus trichiurus (see Annex) has a high cork with thin necks and wide caps on the poles of the egg with the sharp end in Figure 2(b); over the poles of the egg visible 1-I outer transparent shell in the form of a transparent disk, or in the form of a rotating propeller, Figure 1(b), 2(b) - A; G (granules) and P (head cork); cervical samples spiral shape and have 4 rings, 2(b): 1, 2, 3, 4; 3rd ring, considering from the apical end, convex, differs from the previous two rings, Figure 2(b) - 3; 4th last ring, Figure 2(b) 4 and T (germ) is a ring on our image transparent, but sometimes Cartesianism - acts as a liaison between the substrate cervical samples and fourth inner transparent shell of the embryo, Figure 2(b).

On the 2nd traditioanal obolos the e visible spines, Figure 1(a); at the poles of the egg has arms, Figure 1(a); Figure 2: plate spines of different configurations.

2. The way vivo differential diagnosis of trichocera: zoonotic Trichocephalus trichiurus and Trichocephalus suis microstructure eggs according to claim 1., characterized in that Trichocephalus suis (see Annex) has a cork without severe cervical and small caps and not protruding above the surface of the eggs, Figure 3(b) And (cap); Figure 4(b); at the poles eggs 1 outer transparent shell is visible in the form of a fountain translucent microstructures emerging from eggs, Figure 3(a); on the 2nd traditioanal outer shell visible spikes, Figure 5(a); samples are plate-like spines stiletto, Figure 4(a) and Figure 5(a); cervical samples spiral shape with 4 rings, 3(b): 1, 2, 3, 4; 4th ring convex - bond basis cervical samples with the fourth inner transparent shell of the embryo, Figure 3(b) 4 and B (embryo); Figure 5(b).

These microstructural differences in species traits eggs 2 types of trichocera allow way of distinguishing in 100% of cases kinds Trihotel: Trichocephalus trichiurus and Trichocephalus suis without helminthological studies on the opening of domestic and wild pigs, monkeys, or in the absence of helminths when deworming (humans, monkeys, and pigs), or in the study of contamination (pollution) of the external environment germ agents Paris the container zoonoses.

Comparative analysis of the proposed technical solutions to the prototype (flotation, sedimentation and combined) shows that the inventive method differs from the known fact that conduct separate comparison of the morphological features of the eggs and in accordance with our microphotographs (App) is diagnosed with the disease not only to the genus, but the species of helminth Trichocephalus (Trichuris) and trichiurus Trichocephalus suis.

These differences allow to draw a conclusion on the conformity of the proposed technical solution the criterion of "novelty". The features distinguishing the claimed technical solution to the prototype, not identified in other technical solutions, and therefore they provide the claimed technical solution according to the criterion of "significant differences".

Examples of specific performance of the proposed method.

Example. From 25 pigs (before slaughter at the factory) and 5 persons in the Republic of Moldova were taken by well-known methods of flotation, combined and sedimentation feces samples, and selected for the study on 500 eggs Trihotel from each group of infested animals and people. Pre-enlightened parasite eggs mixture of 98.5% solution of glycerol (density 1,256) and 40% lactic acid in a ratio of 1:1 was placed on a glass slide and examined at magnifications of the microscope: 600, 1200 and 900 under immersion.

In% the magnetic resonance studies used we studied for the first time microstructural features in conjunction with micrographs of eggs (Appendix) species (Trichuris) and trichiurus Trichocephalus (Trichuris) suis.

In accordance morphological features: long thin tails and wide caps on the samples at the poles of the egg with a sharp end, Figure 2(b), at the poles of eggs (over samples) visible 1-I outer transparent shell in the form of a transparent disk, Figure 1(b), or in the form of a rotating propeller, Figure 1(b), when the magnification 900, Figure 2(b) - A; cervical samples of eggs spiral shape and have 4 rings, 2(b) - 1, 2, 3, 4; 3rd the ring on the neck of the samples, starting from the apical end, convex, differs from the previous 2 rings, 2(b) - 3; 4-ring acts as a liaison base of the necks of the samples with the fourth transparent shell (germ, eggs, Figure 2(b) - T (embryo); on the 2nd traditioanal shell visible spines; Figure 1(a); at the poles of the egg has arms, Figure 1(a) and figure 2(a): plate spikes of different configurations, Figure 2(a).

In such complex traits and comparing them with our microphotographs diagnosed type Trichocephalus (Trichuris) trichiurus.

In the study of 500 eggs from the second group and using for the first time we studied the microstructural features: cork with short necks and caps, blunt end (the tip) and not protrude above the surface of shell eggs, Figure 3(b) And (cap); at the poles eggs 1 transparent shell is visible in the form of a fountain translucent microstructures emerging from eggs; Figure 3(a); Figure 4(b) cervical samples at the poles spiral shape and have 4 rings, covering the cervix from the cap to the base, Figure 3(b) - 1, 2, 3, 4; 4th ring convex (previous 3 rings of the same width), 3(b) 4, and acts as a liaison between the samples and the fourth inner transparent shell of the embryo eggs; on the 2nd traditioanal shell visible spines, Figure 5(a); samples of eggs visible plate spikes and stiletto, Figure 4(a) and Figure 5(a); after the 1st transparent outer and 3 and 4 th inner transparent shell a good view of the embryo in one part of the egg, Figure 5(b). The combination of these characteristics and their compliance with our microphotographs (Application) allowed the diagnosis of a disease caused by a species Trichocephalus (Trichuris) suis.

After conducting the above studies, pigs were killed and the large intestine and blind intestine was investigated by autopsy method Ceasaria. The pigs were found Mature helminth species Trichocephalus (Trichuris) suis.

Thus, the helminthological studies of samples of faeces from humans and pigs confirmed the data obtained using the proposed method in vivo differential diagnosis to Trichocephalus trichiurus and Trichocephalus suis, and not to the genus (eggs are in the shape of a barrel with two samples at the poles) of the pathogen, since the microstructure of eggs Trihotel from pigs, primates and humans has its species is osobennosti, identified and investigated for the first time (in accordance with the scientific literature) in the world of science.

The use of the proposed method in vivo differential diagnosis microstructure eggs when contamination of the external environment in pigsties, tooprakai, circuses, film platform (when used for filming movies animals: pigs, wild boar, monkeys), beaches (where sometimes wander pigs, and not only Pets, but wild (boar=boar), around private houses with facilities in the yards - toilets - eggs Trihotel from primates, pigs and humans provides over existing: flotation, combined and sedimentation following benefits :

1. Saves time and reduces the complexity of research by eliminating the need for measurements of a large number of eggs: 400 or more (which in practical terms to hold doctors unreal!) eggs trichocera (diagnostics can be run on multiple parasite eggs trapped in the field of view of the researcher).

2. Eliminates the need for studies on the opening of valuable breeding pigs and endangered species of primates in zoos, circuses and menageries production companies: orangutans, gorillas, chimpanzees and other

3. Cost is expressed in the preservation of large financial amounts in the exact appearance of the first diagnosis of the pathogen: Trichocephalus (Trichuris) trichiurus, Trichocephalus suis and, respectively, the conservation and the prevention of death of patients with trihotsefaleza primates and valuable breeds and breeding pigs and more radical desinvasion (contaminated eggs pathogens) external environment and premises, if the source of the invasion were pigs or that the most dangerous (especially for children!), monkey or man.

4. The way vivo differential diagnosis when contamination of the external environment the eggs of trichocephaliasis Trichocephalus trichuris and Trichocephalus suis from monkeys, humans and domestic and wild pigs easily implemented in the laboratory.

5. Ensures 100% accuracy of staging in vivo differential diagnosis not only to the genus, but the species of the parasite.

Sources of information

1. Dinnik NN. Comparison of vlasoglavy human, pig, and some of the lower monkeys. // Thesis...candidate of Sciences. - M - 1941. - 73 C.

2. Kotelnikov GA "Helminthological studies of animals and the environment: a Handbook. - M.: Kolos. - 1984. - 208 S.

3. Brain A.A. Helminths of domestic and wild pigs and is caused by the disease. - M.: Nauka. - 1967. - S-426.

4. Beekeeper VE TO the morphology of the species Trichuris (Trichocephalus) ovis Abildgaard, 1795 T. skrjabini Baskakov, 1924 // Proceedings of all-Russian Institute of helminthology name Ceasaria. - M - 1999. Volume 35. - P.85-120.

5. Beekeeper VE Scanning electron microscopy Trichocephalu (Trichuris) capreoli Artjuch, 1948. // Proceedings of all-Russian Institute of helminthology name Ceasaria. - M - 2000. Volume 36. - S-156.

6. Beekeeper V.E. Thesis...candidate of veterinary Sciences. - M - 2000. - 184 S.

7. Beekeeper VE Patent No. 2396069, application: 2008140245/13, 13.10.2008, date of publication of application: 20.04.2010. // Bul. The Federal service for intellectual property, patents and trademarks of the Russian Federation No. 22, 10.08.2010.

8. Pasechnik V.E. Ultrastructure surface of eggs of the genus Trichocephalus Schrank, 1788 // Second English language International Nematology Simposium of the Societi of Nematologist. - Abstract. - Moscow. - 23-30 August 1997. - P.20.

The way vivo differential diagnosis of zoonotic Trichocephalus trichiurus and Trichocephalus suis microstructure eggs, consisting in the selection of eggs Trihotel on a glass slide, enlightenment them in a mixture of 98.5%-aqueous solution of glycerol density 1,256 and 40%lactic acid, taken in the ratio 1:1, then eggs served a cover slip and viewed under a microscope at magnifications of 600, 1200 and 900 under immersion, characterized in that for the diagnosis of conduct determination of microstructural differences eggs and differentiate types of trichocera by the following features: Trichocephalus trichiurus is high, with thin necks and wide hats cork at the poles of the egg with a sharp end; 1-I outer transparent shell covers all the egg and is visible near the poles in the form of a transparent disk or cap probate is; cervical samples spiral shape and have 4 rings; 3rd ring, starting from the apical end, convex, differs from the previous 2 rings; 4th ring can be transparent or Cartesianism: acts as a liaison between the substrate cervical samples and 4-th shell of the embryo; on the 2nd traditioanal shell visible spines; at the poles eggs are plate-like spines of various configurations; Trichocephalus suis has short tails and hats and not protruding above the surface of the eggs of the cork; at the poles eggs 1 transparent shell is visible in the form of translucent fountain microstructures emerging from eggs; cervical samples spiral shape with 4 rings; 4th convex ring, holding the base of the cervical samples with the inner shell of the embryo; is on the 2nd shell spikes on the plate samples prickles and stiletto.



 

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2 cl, 3 tbl, 3 ex

FIELD: medicine.

SUBSTANCE: method involves preparation of a patient's blood serum sample by drying, grinding and suspending in Vaseline oil; then the sample is examined by infrared spectroscopy in 1200-1000 cm-1 to determine a peak height of absorption bands with maximums 1150, 1130, 1125 cm-1 that is followed by the calculation of the relation of the peak heights with maximum 1150 cm-1 to the peak height with maximum 1130 cm-1 in males and the relation of the peak height with maximum 1150 cm-1 to the peak height with maximum 1125 cm-1 in females. The blood serum sample immediately follows the performed growth surgery, and thereafter in progression, preferentially monthly. If observing increase of the relation of the peak height with maximum 1150 cm-1 to the peak height with maximum 1130 cm-1 in a male patient and the relation of the peak height with maximum 1150 cm-1 to the peak height with maximum 1125 cm-1 in a female patient by the value of 0.50.015 as compared to the relation value derived from the previous sample examination, the recurrent cerebral malignant growth is stated.

EFFECT: use of the method enables stating the postoperative recurrent cerebral malignant growth at the early stages.

2 cl, 3 tbl, 3 ex

FIELD: medicine.

SUBSTANCE: amniotic fluid of pregnant women on their 16-20th week of pregnancy is examined for DNA-binding activity of NF-kB transcription factor; its value 2.9 to 3.7 unit/mg of protein enables diagnosing compensated placental insufficiency, while the value 2.8 unit/mg and lower provides diagnosing decompensated placental insufficiency.

EFFECT: technique ensures higher diagnostic accuracy and specificity and well-timed pathogenetic therapy.

1 tbl, 5 ex

FIELD: medicine.

SUBSTANCE: method for mineral recovery from human connective tissue by low-temperature tissue ignition involving drying of connective tissue kept in 10% formalin in the air for 2-3 days, sample suspension, tissue ignition in porcelain crucibles in an ignition muffle at temperature 450C for 10-12 hours, suspension of the prepared ignited residue.

EFFECT: method enables effective mineral recovery from human connective tissue.

5 dwg, 1 ex

FIELD: medicine.

SUBSTANCE: invention relates to field of veterinary medicine. Method of diagnosing setariasis in livestock includes sampling of animal blood, addition of reagent, settling of sample and detecting under microscope setaria larvae, and as reagent 0.1% acetic acid is applied in quantity 2-3.5 ml per 1 ml of animal blood sample.

EFFECT: invention ensures method simplification, because it is realised without application of centrifuge, and makes it possible to detect alive larvae of setaria with comparatively free field of vision of microscope.

FIELD: personal use articles.

SUBSTANCE: hygienic clothes for pets-mammals includes pants, moisture-repelling insert, the gasket located in the pocket of the moisture-repelling insert, moisture sensor, located between the gasket and the pants, and a means of signaling, coupled with a moisture sensor. The pants are made with the ability to connect with the back of the animal with two upper straps, and with the chest and abdomen of the animal with one lower strap fastened to each other. The lower strap is made hollow to accommodate the wires that connect the moisture sensor with a means of signaling, made as a light source, which means for fastening is made in the form of a triangle of soft tissue, fixed straps at the site of the straps interaction with the front part of the animal's neck on one of the straps, or in a place of connecting the said three straps. The moisture-repelling insert along the pocket contour contains an antimicrobial agent and a positively charged material to capture micro-organisms. The means for attaching additionally comprises a mobile modem, connected to the sensor.

EFFECT: hygienic clothes for pets-mammals is provided.

4 cl, 11 dwg

FIELD: medicine.

SUBSTANCE: invention relates to field of veterinary surgery. Method includes surgical treatment of purulent cavity, installation into cavity of drainage dialysing device for animals, provided with guide-pin for introduction of the device into cavity and plastic fixer, filling of membrane capsule on 1/2 volume with multi-component medicinal solution, which contains dextran solution with molecular weight 4015 kDa, polyethylene oxide 400, imosimase, ethacridine lactate, antibiotic in 1/2 therapeutic dose, lidocaine. Dialysate is replaced 1 time per 48 hours.

EFFECT: method makes it possible to ensure uniform, deep and gradual supply of complex antiseptics into tissues of granulation barrier.

1 tbl, 1 ex

FIELD: medicine.

SUBSTANCE: invention relates to animal breeding, namely to swine breeding. Method lies in the following: in sows analysed are morphological (content of erythrocytes, leukocytes, hemoglobin, content of hemoglobin in one erythrocyte, colour index) and biochemical (reserve alkalinity, content of glucose, total protein, protein fractions, calcium, phosphorus, creatinine, urea, cholesterol, activity of AlAT and AsAT) blood indices and content of hormones (17-oxycorticosteroids general, conjugated 17- oxycorticosteroids, free 17-oxycorticosteroids, adrenalin, noradrenalin) in day urine. Sensitivity of sows to stress factor in idle period is determined by difference in type of regression equation, which reflects structural interposition of main blood components, in case of parabola equation Y(t)=16.862+6.644*t-0.380*t*t animals are referred to stress-resistant, in case of logarithmic equation Y(t)=29.911+2.999*ln(t) - to stress-sensitive. After a month of animals' lactation sensitivity to stress factor is determined by form of regression equation, which reflects structural interrelation of adrenal hormones in animals' urine, in case of linear Y(t)=-8.169+5.371*t, animals are referred to stress-resistant, parabola - Y(t)=8.170-9.845*t+2.879*t*t - to stress-sensitive.

EFFECT: method makes it possible to identify sensitivity of sows to stress-factors more accurately.

2 dwg, 12 tbl, 1 ex

FIELD: medicine.

SUBSTANCE: invention relates to veterinary and is intended for reduction of hyperlipidemia in animal organism. For this purpose into composition of food ration of animals, which are on a atherogenic diet, additionally introduced is one egg of Japanese quail Cotumix cotumix japonica in quantity 20 g per day during three weeks.

EFFECT: invention makes it possible to reduce level of general cholesterol, triglycerides, lipoproteins of very low density (LPVLD) in blood and low-density lipoproteins (LDLP), increase level of high-density lipoproteins (HDLP) in blood in case of hyperlipidemia.

1 tbl, 1 ex

FIELD: medicine.

SUBSTANCE: invention relates to agriculture, in particular to methods of controlling physiological condition of animals. Method of registering start and finish of cows calving consists in the following: at the beginning of calving cow's croup extends, and at the end of calving - narrows. Extension and narrowing of croup is fixed by sensor and electronic unit, which control distance from it to sensor, antenna of receiver-transmitter, connected with computer through its parallel port, controlling computer program and portable receiver-annunciator, which is in use of operator servicing animals. When cow starts calving, controlling program turns on timer. If calving time, which is controlled by computer timer exceeds zoological norm, computer by means of receiver-transmitter and receiver-annunciator informs operator about emergency situation.

EFFECT: application of claimed invention makes it possible to increase cow's safety and index of calf output per a hundred of cows.

1 dwg

FIELD: medicine.

SUBSTANCE: invention relates to field of veterinary obstetrics, in particular, to methods, of uterus cavity washing in animal treatment. Method includes introduction of gynecological instrument into uterus cavity, impact with medicinal solution on material, which is subjected to removal. As gynecological instrument applied is device, made from two pipes, supplying and discharging, connected outside by length, which have on one end common head with mesh dome, under which near butt end of pipes placed is roller with possibility of rotation. In channels of pipes through roller passes cable with fastened on it tampons.

EFFECT: invention insures effective and total removal of pathological content from uterus cavity.

4 cl, 1 tbl, 6 dwg

FIELD: medicine.

SUBSTANCE: invention relates to veterinary science. A method involves introducing anticancer preparations. An anticancer preparation is introduced in an abduction vein offtaking from the tumour whereon a tourniquet is applied in a proximal direction from the introduction point of the preparation. After the preparation is introduced in a distal direction from the injection point, but in a proximal direction from the tumour, another tourniquet is applied. The tourniquets are kept on animal's body for 20-30 minutes. Upon the expiration of time the tourniquets are removed, and the wound is dressed.

EFFECT: method provides higher clinical effectiveness and minimised general toxic effect of the anticancer preparations on organs and tissues of an animal's body.

1 dwg

FIELD: medicine.

SUBSTANCE: invention relates to field of veterinary obstetrics, in particular to instruments, which are applied for washing of uterus cavity in treatment of animals with endometritis. Device is provided with device for movement of tampons into uterus cavity and their extraction back, which consists of two connected by length inlet and outlet tubes, in whose channels placed is cable, with fastened on it tampons from hygroscopic material. Tampons are placed at a distance from each other and made with possibility to move by means of roller. End of one of pipes is a catheter with a common head, with placed on its butt mesh dome and said roller, and roller is placed in oval hole of head with possibility of rotation.

EFFECT: reduction of treatment terms with ensuring its efficiency, in particular, washing of uterus cavity with medicinal solution and removal of pathological contents.

6 dwg

Heating device // 2440829

FIELD: medicine.

SUBSTANCE: group of inventions relates to veterinary. Heating device is used for container with liquid pharmaceutical product, whose viscosity constitutes from 50-500 cP (0.05 - 0.5 Pas) at 4C, which is contained in glass container. Device contains rigid thin-walled casing forming openable chamber for container receiving, source of heat in said chamber, which includes hermetically closed package, containing overcooled metal salt in form of flowing medium or liquid and start device for activation of reaction of heat release by overcooled metal salt. Ratio of metal salt volume to planned product volume constitutes not more than 1:1. Described is device for dosing liquid pharmaceutical product, which contains container placed in heating device.

EFFECT: ensuring portability and autonomy of equipment.

17 cl, 5 dwg

FIELD: agriculture, veterinary medicine.

SUBSTANCE: the suggested method deals with selecting the samples and their transportation for further testing. Liquid sample should be applied into a sterile syringe due to vacuum through a pipette for recto-cervical insemination in cows. Pipette and a syringe should be connected with a coupling the length of which enables to change the angle of syringe's inclination against a pipette, moreover, a free end of pipette should be applied at an angle in cavitary organ. After selecting the samples on should disconnect the coupling and the pipette against the syringe, and its cannula should be covered with a cap. The present innovation enables to increase the safety of working process while selecting liquid samples out from remote sections of cavitary organs followed by their transportation for further laboratory investigation.

EFFECT: higher efficiency of selection.

1 dwg

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