Method of diagnosing biliary tract dysfunction in children

FIELD: medicine.

SUBSTANCE: invention relates to field of medicine, namely to pediatrics. In order to diagnose biliary tract dysfunction in children acetic and propionic acids are determined in blood and saliva by gas-chromatographic method. If concentration of any of two acids in blood: acetic in the range more than 0.3264 to 1.1000 mmol/l, propionic in the range more than 0.0274 to 0.0990 mmol/l and/or if concentration of any of two acids in saliva: acetic in the range more than 0.0683 to 0.5900 mmol/l, propionic in the range more than 0.0066 to 0.0430 mmol/l, biliary tract dysfunction is diagnosed.

EFFECT: method makes it possible to diagnose biliary tract dysfunction in children.

3 ex, 3 tbl

 

The invention relates to medicine, namely to Pediatrics, gastroenterology and clinical laboratory methods.

Polymorphism of the clinical symptoms of dysfunction of the biliary tract (DBT) is so pronounced that the diagnosis of the disease may represent a rather complicated problem.

To diagnose DBT in children using clinical symptoms and the number of studies: screening tests and lookup methods of diagnosis. To include screening blood chemistry for hepatic complex, pancreatic enzymes in the blood and urine, ultrasound (us) and esophagogastroduodenoscopy, and to clarify - ultrasound assessment of the functional condition of the gallbladder; endoscopic ultrasonography; endoscopic retrograde cholangiopancreatography with intrahepatically by manometry; dynamic cholescintigraphy. Any of the above survey methods has several advantages and has its diagnostic value. Only aggregate picture of all diagnostic complex allows to establish the diagnosis (Ursula NI Problem of functional disorders of the biliary tract in children and their correction // Russian medical journal. - C, No. 3. - 2003. - Page 16, the Guide outpatient Pediatrics / edited Bra. - M.: GEOTAR-Media, 2006. - S-203).

The manometry of the sphincter of Oddi is carried out only in specialized hospitals during esophagogastroduodenoscopy and is not used for mass screening and preventive examinations due to the complexity of this instrumental study and possible complications in patients. Known contraindications to esophagogastroduodenoscopy, a possible failure of the patient or the parent of a sick child from this instrumental study, which limits the scope of application of this method for the diagnosis DBT in children. Method radioisotope scanning is complex, time consuming and has a radial load on the child's body. For its realization requires expensive equipment, designated areas, the availability of expensive pharmaceuticals. Currently, the technique of duodenal intubation is not recommended for examination of children due to the negative impact on the psyche of the child and the difficulties of interpretation of the obtained results. Retrograde pancreaticobiliary complex and has a radial load on the body of the child, it is prescribed for persistent disease and treatment failure. Methods additional studies are most informative are the results of the ultrasound with the study of motor function of the gallbladder. For said the tion of the motor-evacuation function of the gallbladder is estimated by the degree of contractility after cholagogue Breakfast (Mach VM, Romasenko L.V., Turko T.V. // Russian medical journal. - 2007. - v.9, No. 2).

Simultaneous ultrasound in the diagnosis DBT not very informative and mainly reflects the anatomical structure of the body. Ultrasound to study the function of the gallbladder is not used for mass screening and preventive examinations due to the complexity of this instrumental study. The ultrasound results depend on the quality of equipment and qualifications of a physician (the subjective factor). The disadvantages of ultrasound as a diagnostic DBT in children is a visual assessment, using qualitative and semi-quantitative parameters. This reduces the accuracy of diagnosis DBT children.

We were determined by gas chromatography the concentration of acetic and propionic acids in the blood and saliva of healthy and sick children with DBT. We found a statistically significant increase in mean values of concentrations of acetic acid in the blood and acetic and propionic acids saliva in children with DBT compared with values in healthy children (table 1).

Table 1
The concentrations of acetic (C2) and propionic (C3) acids in the blood and saliva of patients with DBT (mmol/l)
/td> The concentration of acids in patients with DBT M±m (n=45)The difference between the average value and the standard errors of the concentration of acids in patients with DBT
BiologicalWith2NWWith2NW
Blood0,3685±0,04210,0316±0,00420,32640,0274
Saliva0,0838±0,01550,0078±0,00120,06830,0066

The most informative are measures of M-m the difference between the average concentration and standard error of average values of the concentrations of acetic and propionic acids in the blood and saliva in children with DBT. These indicators were selected as the most informative diagnostic criteria.

The Rome consensus II posits that functional gastrointestinal disease is "variable combination of chronic or recurrent symptoms not explained by structural or biochemical changes. Unlike somatic pathology criteria function the national digestive diseases based on symptoms. Changes in clinical and biochemical blood tests in patients with uncomplicated inflammation or cholelithiasis dysfunction of the biliary tract, usually absent.

The main fraction of volatile fatty acids (AGV) absorbed from the lower segment of the small and large intestine and enters the portal vein, where the concentration of the AGV in the internal environment of the body to the maximum. A significant part of the AGV is burned in peroxisome hepatocytes. In the blood, flowing from the liver, the concentration of the AGV significantly less (Beloborodov NV, Beloborodov CM. Metabolites of anaerobic bacteria (volatile fatty acids) and the reactivity of the microorganism // Antibiotics and chemotherapy, 2000. - N 2. - P.28-36).

The idea of the dysfunction of the gallbladder as a purely functional disorders currently under revision. Not only when optomotry, but when hypermotor forms of dysfunction are organic changes at the level of hepatocytes, which is sort of the primary factor contributing to the infringement activities not only inside, but also extrahepatic biliary tract, including the gallbladder. This disease represents a certain type of cholestasis. Violation of the rhythm of the flow of bile into the intestine reduces the bacteriostatic properties of the upper parts of the gastrointestinal tract. DBT accompanied violations is receiving processes of digestion and absorption and excessive growth of microflora in the gut (Manual of outpatient Pediatrics / edited Bra. - M.: GEOTAR-Media, 2006. - S-203). When DBT in connection with cholestasis and reduction bacteriostatic action of bile develops hyperboloidal intestinal microflora, which leads to an increase in the concentration of acetic acid in the blood and saliva. In addition, when DBT possibly disturbed function of hepatocytes on oxidation AGV, which also leads to an increase of their concentration in blood and saliva.

The technical result is achieved by including additional clinical and survey method for the diagnosis of dysfunction of the biliary tract in children involves identifying children acetic and propionic acids in the blood and saliva at a concentration of either of the two acids in the blood: more acetic 0,3264 to 1,1000 mmol/l propionic more 0,0274 to 0,0990 mmol/l and/or at a concentration of either of the two acids in saliva: acetic more 0,0683 to 0,5900 mmol/l propionic more 0,0066 to 0,0430 mmol/l diagnosed with dysfunction of the biliary tract.

The diagnostic method is performed as follows.

Analyze the data of anamnesis, physical, instrumental and laboratory examination of the patient.

After pre-rinsing of the mouth with boiled water intake saliva by direct allocation of patients into the vial. Blood sampling is an accepted way. Preparation of blood samples and the young for conventional chromatography by the method of solvent extraction. Gas-liquid chromatography for the quantitative determination of acetic, propionic and butyric acids performed on a gas chromatograph "Crystallux-4000 with quartz capillary column HP-FFAP a length of 50 m, an inner diameter of 0.3 mm and with a flame ionization detector under isothermal conditions at a temperature of 200°C. the carrier Gas - helium (Beloborodov NV, A. V., Boyko NB and other Diagnosis of anaerobic infections in children by chromatography. Methodical recommendations. - Reg. No. 96/130. - The state register of new medical technologies. - Issue 3). Identification and quantitative determination of acetic and propionic acids perform using analytical standards.

The practical implementation of the proposed method does not require complex methods of research, so it can be used in all children with clinical signs DBT, in clinics and in addition to the diagnostic algorithm in hospitals. The use concentration of the acid increases the accuracy of diagnosis through the use of quantitative parameters reduces the time required for diagnosis, this diagnosis is carried out without complications.

To prove the adequacy of the diagnosis of dysfunction of the biliary tract in children indicators acetic and propionato acids in the blood and vinegar is Oh acid in saliva conducted a comparison of diagnostic results by the proposed method and the conventional method. Diagnosis DBT in children has established a standard method, according to surveys in accordance with the Manual of outpatient Pediatrics / edited Bra. - M.: GEOTAR-Media, 2006. - S-203/ using clinical, instrumental and laboratory methods of examination.

We studied 45 patients with dysfunction of the biliary tract aged 6-17 years. The control group consisted of 8 healthy children. The oral cavity in all children was sanitized.

The proposed method allowed the diagnosis in 38 of the 45 children with dysfunction of the biliary tract (the match with a final diagnosis of 84%).

Thus, the acetic acid concentration in blood and saliva can be used to diagnose dysfunction of the biliary tract in children.

Examples of the method in children with dysfunction of the biliary tract: four examples are presented in table 2, and two of the four examples described in more detail below.

Table 2
Examples of values acetic (C2) and propionic (C3) acids in blood and saliva in children with DBT (mmol/l)
The concentrations of acetic and propionic acids in the of patients with DBT
K.A.G.K.S.M.ZH.A.
Biological fluidWith2NWWith2NWWith2NWWith2NW
Blood0,14000,06900,31000,07200,89000,05600,08800,0440
Saliva0,02000,00250,00220,00800,28000,00640,24000,0120

Clinical example 1. Kuznia Alla (K.A.), 9 years. Complaints of abdominal pain paroxysmal character within 1 year, regardless of the meal, occasionally nausea, weakness and fatigue. Appetite is reduced. It was noted increased wosb the need. Objective: tongue coated at the root. The abdomen is soft, moderately painful in the upper sections. The liver even royalty of 1.5 cm from the edge of the costal arch. Was determined soreness at the point of Kera. Chair regular.

Additional tests: ultrasound examination of abdominal gallbladder with a narrow S-shaped neck, a small amount exogenous mist; biochemical blood test: whole protein 88,0; glucose 4,8; bilirubin total 4,0; no direct; ALT 15; ACT 18; amylase 12; alkaline phosphatase 1,5; thymol turbidity test 2,8; CRP - negative. General analysis of blood without pathology.

The results of determination of acetic and propionic acids (mmol/l) in the blood - 0,1400 (C2); 0,0690 (C3); in the saliva - 0,0200 (C3); 0,0025 (C3). Clinical signs and concentration of propionic acid in the blood within more 0,0274 to 0,0990 mmol/l diagnosed with dysfunction of the biliary tract. Final diagnosis: dysfunction of the biliary tract coincides with the diagnosis of the claimed method.

Clinical example 2. Garin Cyril (AS), 15 years. Complaints of abdominal pain, often aching, sometimes paroxysmal after eating and physical activity. First abdominal pain appeared three years ago. Occasionally nausea and vomiting, headache. A good appetite. Boy hyperexcitability. Objective: the root of the tongue with a white coating, the abdomen is soft, painful is the right hypochondrium. The liver is palpated from under the edge of the costal arch 0.5 cm, gallbladder symptoms weakly positive. A chair with a tendency to constipation. Additional tests: ultrasound SSB - liver without pathology of the gall bladder with a bend in the neck, a small amount exogenous mist. Motor function of the gallbladder was normal. Biochemical blood test: whole protein 82,6; glucose 5,0; total bilirubin 4,0; no direct; ALT 24; ACT 26; amylase 12; alkaline phosphatase 1,9; thymol turbidity test 3,3; CRP - negative. General analysis of blood without pathology.

The results of determination of acetic and propionic acids (mmol/l): blood - 0,3100 (C2), 0,0720 (C3), in a saliva - 0,0022 (C2), 0,0080 (C3). Clinical characteristics and concentrations of propionic acid in the blood more 0,0274 to 0,0990 mmol/l; propionic acid in the saliva within more 0,0066 to 0,0430 mmol/l diagnosed with dysfunction of the biliary tract. Final diagnosis: dysfunction of the biliary tract coincides with the diagnosis of the claimed method.

Examples of the method in healthy children: four examples are presented in table 3, and one of them is described below.

Clinical example 3. Koval Alena (K.A.), 11 years. No complains. Consulting a gastroenterologist was not addressed. From the active survey revealed that abdominal pain, dyspeptic symptoms in girls are not noted is ALOS. A good appetite. Objectively: the patient is in satisfactory condition. Skin, mucous net. Tongue moist, pink, clear. The abdomen is tender to deep palpation, painless in all departments. The liver is palpated at the edge of the costal arch, gallbladder symptoms negative. Chair in norm. Ultrasound of abdominal pathology was not detected. Biochemical analysis of blood and clinical analyses within age norms.

The results of determination of acetic and propionic acids (mmol/l): blood - 0,0084 (C2), 0,0046 (C3), in a saliva - 0,0076 (C2), 0,0017 (C3). Clinical characteristics and concentrations of acetic and propionato acids in the blood (less 0,1400 and 0,0274 mmol/l, respectively) and saliva (less 0,0200 and 0,0120 mmol/l, respectively) were diagnosed as healthy. Final diagnosis: healthy coincides with the diagnosis of the claimed method.

Table 3
Examples of values acetic (C2) and propionic (C3) acids in blood and saliva in healthy children (mmol/l)
The concentrations of acetic and propionic acids in patients with DBT
K.A. G.K.S.M.ZH.A.
Biological fluidWith2NWWith2NWWith2NWWith2NW
Blood0,00840,00460,00190,00050,00060,00490,00270,0003
Saliva0,00760,00170,00410,00050,00690,00370,00160,0001

Thus, the claimed method is objective and informative, and using clinical signs of disease and the acetic acid concentration in blood and saliva, it is possible to diagnose the presence of dysfunction of the biliary tract. The diagnostic method allows to increase the objectivity and accuracy of diagnostics for the account and the use of quantitative parameters without any unwanted side effects in children.

Method for the diagnosis of dysfunction of the biliary tract in children by examination of the patient, wherein the gas chromatography method determines in blood and saliva of acetic and propionic acid at a concentration of either of the two acids in the blood: acetic within more 0,3264 to 1,1000 mmol/l propionic within more 0,0274 to 0,0990 mmol/l and/or at a concentration of either of the two acids in saliva: acetic within more 0,0683 to 0,5900 mmol/l propionic within more 0,0066 to 0,0430 mmol/l diagnosed with dysfunction of the biliary tract.



 

Same patents:

FIELD: medicine.

SUBSTANCE: invention relates to medicine and deals with method of controlling check meter of glucose. Method can be realised in stages, which consist in storing multitude of indices of glucose in memory of check meter, each of glucose indices containing time mark and order code with time mark containing date and time of glucose index registration and order code showing order, in which multitude of glucose indices is stored in memory of check meter; determination whether successively stored glucose indices contain time marks in chronological order, corresponding to order code; and showing that setting of date and time in check meter is not exact, if there exist successively stored glucose indices, which contain time marks, which do not correspond to order index.

EFFECT: invention ensures higher accuracy with minimal complexity.

25 cl, 9 dwg

FIELD: medicine.

SUBSTANCE: method involves preparation of a patient's blood serum sample by drying, grinding and suspending in Vaseline oil; then the sample is examined by infrared spectroscopy in 1200-1000 cm-1 to determine a peak height of absorption bands with maximums 1150, 1130, 1125 cm-1 that is followed by the calculation of the relation of the peak heights with maximum 1150 cm-1 to the peak height with maximum 1130 cm-1 in males and the relation of the peak height with maximum 1150 cm-1 to the peak height with maximum 1125 cm-1 in females. The blood serum sample immediately follows the performed growth surgery, and thereafter in progression, preferentially monthly. If observing increase of the relation of the peak height with maximum 1150 cm-1 to the peak height with maximum 1130 cm-1 in a male patient and the relation of the peak height with maximum 1150 cm-1 to the peak height with maximum 1125 cm-1 in a female patient by the value of 0.5±0.015 as compared to the relation value derived from the previous sample examination, the recurrent cerebral malignant growth is stated.

EFFECT: use of the method enables stating the postoperative recurrent cerebral malignant growth at the early stages.

2 cl, 3 tbl, 3 ex

FIELD: medicine.

SUBSTANCE: method involves preparation of a patient's blood serum sample by drying, grinding and suspending in Vaseline oil; then the sample is examined by infrared spectroscopy in 1200-1000 cm-1 to determine a peak height of absorption bands with maximums 1150, 1130, 1125 cm-1 that is followed by the calculation of the relation of the peak heights with maximum 1150 cm-1 to the peak height with maximum 1130 cm-1 in males and the relation of the peak height with maximum 1150 cm-1 to the peak height with maximum 1125 cm-1 in females. The blood serum sample immediately follows the performed growth surgery, and thereafter in progression, preferentially monthly. If observing increase of the relation of the peak height with maximum 1150 cm-1 to the peak height with maximum 1130 cm-1 in a male patient and the relation of the peak height with maximum 1150 cm-1 to the peak height with maximum 1125 cm-1 in a female patient by the value of 0.5±0.015 as compared to the relation value derived from the previous sample examination, the recurrent cerebral malignant growth is stated.

EFFECT: use of the method enables stating the postoperative recurrent cerebral malignant growth at the early stages.

2 cl, 3 tbl, 3 ex

FIELD: medicine.

SUBSTANCE: amniotic fluid of pregnant women on their 16-20th week of pregnancy is examined for DNA-binding activity of NF-kB transcription factor; its value 2.9 to 3.7 unit/mg of protein enables diagnosing compensated placental insufficiency, while the value 2.8 unit/mg and lower provides diagnosing decompensated placental insufficiency.

EFFECT: technique ensures higher diagnostic accuracy and specificity and well-timed pathogenetic therapy.

1 tbl, 5 ex

FIELD: medicine.

SUBSTANCE: method for mineral recovery from human connective tissue by low-temperature tissue ignition involving drying of connective tissue kept in 10% formalin in the air for 2-3 days, sample suspension, tissue ignition in porcelain crucibles in an ignition muffle at temperature 450°C for 10-12 hours, suspension of the prepared ignited residue.

EFFECT: method enables effective mineral recovery from human connective tissue.

5 dwg, 1 ex

FIELD: medicine.

SUBSTANCE: method for mineral recovery from human connective tissue by low-temperature tissue ignition involving drying of connective tissue kept in 10% formalin in the air for 2-3 days, sample suspension, tissue ignition in porcelain crucibles in an ignition muffle at temperature 450°C for 10-12 hours, suspension of the prepared ignited residue.

EFFECT: method enables effective mineral recovery from human connective tissue.

5 dwg, 1 ex

FIELD: medicine.

SUBSTANCE: what is conducted is 10-minute hypoxic loading test (LT) using oxygen poor (up to 10-11%) normobaric gas mixture with reoxygenation time recorded and physiological parameters controlled. It is followed by calculating a functional deviation coefficient (Cf) in standard units by formula: Cf=ΔRR/10+ΔHR/10+ΔSBP/10+ΔDBP/10+ΔSaO2/10+Tr/60, wherein ΔRR/10 is a gradient of respiratory rate (respiratory rate increment for a hypoxic time divided by 10), standard units; ΔHR/10 is a gradient of heart rate (heart rate increment for a hypoxic time divided by 10), standard units; ΔSBP/10 is a gradient of systolic blood pressure (increase/decrease of systolic blood pressure (mm Hg) for a hypoxic time divided by 10), standard units; ΔDBD/10 is a gradient of diastolic blood pressure (increase/decrease of diastolic blood pressure (mm Hg) for a hypoxic time divided by 10), standard units; ΔSaO2/60 is a gradient of SaO2 (decrease of arterial oxygen saturation (%) for a hypoxic time divided by 10), standard units; Tr/60 is a gradient of reoxygenation (a reoxygenation time (sec.) divided by 60), standard units. If Cf≤6.5, an optimum level of functional reserves is stated; the value 6.5<Cf≤12 means decrease of functional reserves; while depleted functional reserves are shown by the value Cf>12.

EFFECT: use of the method enables the objective assessment of manifestation of the deviations, including the pre-clinical one for the purpose of directed correction.

3 ex

FIELD: medicine.

SUBSTANCE: what is involved is fibrogastroduodenoscopy with biomaterial sampling by biopsy of the duodenal mucosa. The biopsy material is morphologically analysed. The presence of eosinophilic leukocytes and retention cysts of pyloric glands enables diagnosing lamblia invasion with underlying chronic duodenitis.

EFFECT: invention provides higher objectivity of the diagnostic technique for lamblia invasion with underlying chronic duodenitis.

1 ex

FIELD: medicine.

SUBSTANCE: invention relates to field of medicine, namely to traumatology and orthopedics. In order to estimate intensity of destructive processes in case of knee joint traumatic injury and prediction of development of post-traumatic arthrosis, content of oxyprolene in determined in rats after modelling of knee joint trauma. Content of protein-bounded oxyprolene in plasma higher than 75 mcmol/l with simultaneous increase of free fraction of oxyprolene higher than 16 mcmol/l testified to active reparation at the background of continuing intensive destruction of collagen structures resulting in deforming osteoarthrosis.

EFFECT: method increases accuracy of estimation of destructive processes intensity in case of traumatic injury of knee joint and prediction of development of post-traumatic arthrosis due to determination of quantitative and fractional content of oxyprolene.

FIELD: medicine.

SUBSTANCE: invention relates to field of medicine, namely to neurology, immunology and occupational pathology. Rheoencephalography with hypercapnic test are performed, visual evoked potentials (VEP), encephalography (EEG) are registered, level of antibodies to protein S100, level of immunoglobulin G in blood serum are determined. Canonical value is calculated by formula: Cv=3.18-0.38×a1-1.61×a2-0.71×a3+0.93×a4+1.19×a5-0.81×a6, where Cv is canonical value; -0.38; -1.61; -0.71; 0.93; 1.19; -0.81 are discriminant coefficients; 3.18 is constant; a1 is intensity of blood supply in frontomastoid pool during hypercapnic test in ohm; a2 is amplitude of peak N1 of VEP in right occipital lead in mcV; a3 is level of antibodies to protein S100 in conv. units; a4 is level of immunoglobulin G in g/l; a5 is presence or absence of nidus of pathological activity on EEG: 0 - no, 1 - present; a6 is degree of expression of diffuse changes by EEG; 0 - no, 1 - mild, 2 - moderate, 3 - expressed, 4 - severe. If Cv is lower than the constant, early manifestations of chronic mercury intoxication are diagnosed, if Cv is higher or equals the constant, chronic mercury intoxication of the first.

EFFECT: method extends arsenal of means for diagnostics of early manifestations of chronic mercury intoxication.

1 tbl, 3 ex

FIELD: medicine.

SUBSTANCE: invention relates to medicine, in particular to dentistry and is intended for estimation of esthetic results of dental treatment. Essential esthetic face characteristics: symmetry of features (S), state of teeth in esthetically important zone (ST), naturalness of mimics (M) and smile (S) are estimated before and after treatment by formula: Ie=(S+ST+M+S):4, where Ie is index of total esthetic result of dental treatment. Estimation in points of each face characteristics is performed on the basis of bilateral contact expertise by scheme "doctor-patient". Quantity of points of each essential face characteristics - feature symmetry, state of teeth in esthetically important zone of oral cavity, mimics naturalness, smile naturalness is determined on the basis of reduction of possible highest mark in 100 points, where one point corresponds to one percent. Total esthetic result is estimated as good, when Ie equals 95-100 points, as satisfactory - from 90 to 94 points and Ie is considered to be unsatisfactory if Ie is less than 90 points.

EFFECT: method makes it possible to increase accuracy of estimation of dental treatment results due to bilateral contact expertise by scheme "doctor-patient" and system of calculation of value of estimation of each essential face characteristics.

3 ex

FIELD: medicine.

SUBSTANCE: invention relates to field of medicine, namely to traumatology and orthopedics. In order to estimate intensity of destructive processes in case of knee joint traumatic injury and prediction of development of post-traumatic arthrosis, content of oxyprolene in determined in rats after modelling of knee joint trauma. Content of protein-bounded oxyprolene in plasma higher than 75 mcmol/l with simultaneous increase of free fraction of oxyprolene higher than 16 mcmol/l testified to active reparation at the background of continuing intensive destruction of collagen structures resulting in deforming osteoarthrosis.

EFFECT: method increases accuracy of estimation of destructive processes intensity in case of traumatic injury of knee joint and prediction of development of post-traumatic arthrosis due to determination of quantitative and fractional content of oxyprolene.

FIELD: medicine.

SUBSTANCE: invention relates to medicine and can be used for examination and diagnostics of biological object or its part. Method of examination and diagnostics includes the following stages: image of biological object is obtained, obtained image is calibrated and standardised, computed morphodensitometric analysis is performed. For this purpose zones of interest are identified on obtained image, required ranges of charcteristics of signal intensity are determined by fixed distribution of characteristics of irradiation interaction with substance on image, graphic presentation of distribution of intensities of image elements is formed with possibility of superposition of graphic presentation on image. After that, morphometric and densitometric parameters of image are determined, said parameters of examined object are compared with analogous parameters of reference group and decision about condition of examined object is taken on the basis of comparison results. When image of biological object is obtained, shooting of examined object is performed with additional application of illuminator with changeable emission spectrum. Source of illumination is moved on circumference around object of examination and angle of ray incidence is changed discretely at each following circle due to movement of illuminator, shooting is performed and series of object images in different spectrums of illumination for each position of illumination source is obtained, calibration of light intensity is performed in different spectrums of illumination. After that, several pictures of image are accumulated with further obtaining by said pictures average image for each version of illuminator spectrum separately, graphic presentation of distribution of intensities of image elements is performed in form of histogram. Histogram is performed with possibility of linearise and expanding in automatic, interactive modes.

EFFECT: application of claimed invention will make it possible to increase accuracy and hardness of obtained images of examined objects, and due to it obtain more objective picture of changes in biological object at the level of its structure.

7 cl

FIELD: medicine.

SUBSTANCE: invention relates to field of medicine, namely to phthisiology. In order to carry out differential diagnostics X-ray examination of patient's lungs and laboratory test of blood parameters are performed. In case if inhomogeneous and highly-intensive infiltration of lung tissue is detected, level of cortisol in blood serum is additionally determined, and if its value is from 808 nmol/l to 2500 nmol/l, caseous pneumonia is diagnosed, if value is from 159 nmol/l to 718 nmol/l, infiltrative tuberculosis of lungs is diagnosed.

EFFECT: method increases accuracy of differential diagnostics of caseous pneumonia due to determination of level of cortisol in blood serum.

2 ex

FIELD: medicine.

SUBSTANCE: stage of atopic dermatitis in children is diagnosed by immunobiological assay of patient's peripheral blood. A degree of lymphocyte expression of CD95+ markers is evaluated. If the CD95+ value exceeds 60.09% enables diagnosing an acute stage of the disease, while the CD95+ values 20.33% to 60.09% shows a chronic stage.

EFFECT: well-timed prediction of the clinical course ensured by accuracy and acceleration of dianosis of transmission of the acute stage of atopic dermatitis in children into the chronic stage.

1 tbl, 2 ex

FIELD: medicine.

SUBSTANCE: invention refers to medicine, namely endocrinology and diabetology. The clinical course of diabetes mellitus is predicted by evaluating the following risk factors: tongue rolling, myopia, jug ears, joint hypermobility, platypodia, astenic body, diagonal fold of the earlobe, high skin elasticity, varicosity, visceroptosia, height/span > 1.1, clinodactyly, mitral valve prolapse, renal cysts, Gothic palate, chest distortion, biliary tract deformation, arachnodactylia, false left-ventricular chordas. If the patients suffering type 1 diabetes mellitus shows 5 or more indicators, a high risk of developing diabetic nephropathy and arterial hypertension is predicted. If the patients suffering type 2 diabetes mellitus shows 5 or more indicators, a high risk of developing ischemic cardiac disease is predicted. If observing 4 or less indicators in the patients suffering types 1 and 2 diabetes mellitus, a minimum risk of the unfavourable clinical course of diabetes mellitus is predicted.

EFFECT: method enables predicting the clinical course of diabetes mellitus in the patients with types 1 and 2 diabetes mellitus on the basis of the indicators of increased dysplastic stigmation.

3 ex

FIELD: medicine.

SUBSTANCE: invention relates to field of medicine, in particular to pediatrics. When child is one month old, their somatic and neurological status is estimated and equation of regression is calculated: 42.423-4.373 ×No of group - 0.524 × sex - 0.972 × retardation of development of motor functions - 1.264 × intrauterine infection - 0.763 × allergic dermatitis- 1.212 × disembriogenesis stigmas + 1.807 × advancing development of communicative functions, where - No of group - 2 for group of children, who receive and No of group 3 for group of children who do not receive additional medical-corrective procedures; sex - 1 female, 2 - male; retardation of development of motor functions - 0 no, 1 present; intrauterine infection - 0 no, 1 present; allergic dermatitis - 0 no, 1 present; disembriogenesis stigmas - 0 no, 1 present; advancing development in communicative functions - 0 no, 1 present. Equation is calculated twice - for situation of application and absence of carrying out complex of medical-corrective procedures. Obtained values for both groups are compared and necessity of carrying out medical-psychological-pedagogical programme is determined.

EFFECT: method makes it possible to obtain objective estimation of age development of child and determine necessity of carrying out correction of their development.

2 ex

FIELD: medicine.

SUBSTANCE: invention relates to field of medicine, in particular to endosurgery. In order to perform early diagnostics of acute pancreatitis after endoscopic transpapillary interventions, general clinical and physical examination, biochemical analysis of patients' blood are performed. In the course of post-operative examination "index of pancreatitis" (IP) is determined by formula: Hn=((Ap/An)x(PS1/PS0)×(L1/L0)×(PC+1))/4, where: Ap are values of patient's blood amylase; An are maximal values of blood amylase in norm; PS0 is average value of patient's pulse before endoscopic transpapillary interventions; PS1 is average value of pulse after endoscopic transpapillary interventions; L0 is number of blood leukocytes before endoscopic transpapillary interventions; L1 is number of blood leukocytes after endoscopic transpapillary interventions; PC is pain coefficient. Degree of pain syndrome expression is evaluated in points: 0 points - pain in abdomen is absent at rest and palpation; 1 point - pain in abdomen is present at palpation and is absent at rest; 2 points pain in abdomen is present at rest and increases at palpation; 3 points - together with pain syndrome, positive peritoneal symptoms are present. If IP≥ 1, acute postoperative pancreatitis is diagnosed.

EFFECT: method increases accuracy of early diagnostics of acute pancreatitis after endoscopic transpapillary interventions due to determination of totality of interconnected informative criteria such as value of amylase, value of pulse, number of leukocytes in blood, pain coefficient.

2 ex

FIELD: medicine.

SUBSTANCE: invention relates to field of medicine. In order to predict risk of development of occupational bronchopulmonary pathology, venous blood is sampled, genetic material is isolated, polymerase chain reaction is performed with specific primers, nucleotide sequence is determined and on the basis of this polymorphic versions of gene of matrix metalloproteinase 1 polymorphism 1607 delG are determined. In carrying out polymerase chain reaction specific primers MP1-F 138F BIOTIN - GCG TCA AGA CTG ATA TCT TAC TC A TAA ACA ATA and MP1-R 138R ACA TgT TAT gCC ACT TAg ATg Agg AAA are used. After carrying out polymerase chain reaction, reaction of pyrosequencing with application of specific primer MP1-S 138S gTA gTT AAA TAA TTA gAA Ag and detection of nucleotide sequence are performed. After that, comparison of obtained nucleotide sequence with reference sequences is carried out and obtained program is used to determine version of polymorphism 1607delG in nucleotide sequence, by which risk of development of occupational bronchopulmonary pathology is predicted.

EFFECT: method increases rate of carrying out prediction of risk of development of occupational bronchopulmonary pathology.

3 dwg, 3 ex

FIELD: medicine.

SUBSTANCE: invention relates to field of medicine, namely to pediatrics. In order to estimate level of bronchial asthma control in children analysis of night symptoms in patient and determination of characteristics of lung function are carried out. Frequency of night attacks, peak rate of exhalation in child, mother and father are evaluated. Additionally determined are: child's, mother's and father's age and height. Taken into account are: content of total Immunoglobulin E in child, child's chest circumference, age at the beginning of disease, duration of disease, duration of anti-inflammatory therapy, coefficient of gender identity, volume of performed anti-inflammatory therapy, treatment with inhalation glucocortocosteroids separately and together with inhalation long-acting β2-agonists, application of cromones, application of antileukotriene drugs, passive or active smoking, atopic dermatitis, allergic rhinitis, sensibilisation to household, epidermal and pollen allergens. Level of bronchial asthma control is estimated by value of coefficient obtained in application of discriminant analysis.

EFFECT: method makes it possible to increase accuracy of estimation of level of bronchial asthma control in children due to determination of lung function characteristics taking into account contribution of inheritance factor and time factor, volume of performed treatment, accompanying atopic diseases, spectrum of child's sensibilisation, individual anthropometric characteristics of child and their parents.

3 ex

FIELD: medicine, clinical toxicology.

SUBSTANCE: at patient's hospitalization one should gather the data of clinical and laboratory values: on the type of chemical substance, patient's age, data of clinical survey and laboratory values: body temperature, the presence or absence of dysphonia, oliguria being below 30 ml/h, hemoglobinuria, erythrocytic hemolysis, exotoxic shock, glucose level in blood, fibrinogen and creatinine concentration in blood serum, general bilirubin, prothrombin index (PTI), Ph-plasma, the state of blood clotting system. The state of every sign should be evaluated in points to be then summed up and at exceeding the sum of points being above "+20" one should predict unfavorable result. At the sum of "-13" prediction should be stated upon as favorable and at "-13" up to "+20" - prediction is considered to be doubtful.

EFFECT: higher accuracy of prediction.

2 ex, 3 tbl

Up!