Method for simulating prevention of hospital strain formation
SUBSTANCE: method involves the intramuscular introduction of the preparations Galavite 200 mcg/mouse and Lidocaine 8 mcg/kg of body weight once a day for three days running every 24 hours to experimental animals CBA line mice suffering a burning injury with underlying mycotic and bacterial infections accompanied by anti-infectious protection suppression.
EFFECT: invention enables creating a model of hospital strain formation which is applicable for studying the variations of the properties of opportunistic microorganisms to increasing virulence and antibiotic resistance.
The proposed method relates to medicine, namely to Microbiology, immunology and epidemiology, and can be used to simulate prevent the formation of hospital strains.
The problem of nosocomial infection is one of the most serious, as it has a significant impact on the duration of hospitalization, hospital mortality and, ultimately, the cost of treatment. The main reason for the development of nosocomial infections are hospital strains of conditionally pathogenic microorganisms, which are formed in stationary conditions.
Until recently, measures of nosocomial infections was based on the struggle with already incurred by immunocompromising patients and medical staff, inflammatory infections. However, it is possible to prevent the occurrence of nosocomial infections in the formation of hospital strains of pathogens by using modern advances in medicine, in particular, by applying the methods of preventing formation of hospital strains of pathogens.
Literature data about how microbiological monitoring of nosocomial infections have significant shortcomings.
The closest prototype is "a Method for the diagnosis of nosocomial microbe who's associations microbiological monitoring in the framework of the epidemiological surveillance of septic infections (Patent for invention №2298037).
One of the fundamentally important shortcomings proposed in the literature is the study of the properties of pathogens at the level of strains of microorganisms. It is known that populations of all living beings, non-uniform (heterogeneous) on various biological characteristics, which ensures the reliability of the living systems under changing conditions of existence. According to E. Belyakova (1987), the most important driving force of epidemiology (infectious) process and one of the main conditions for the existence of parasitic systems is the heterogeneity of the population of the microorganism and the microorganism (host-parasite).
Set (Vli, Pavolotsky, CIA and other Heterogeneity of microbial populations, M., 2008, 154 C.)that populations of freshly isolated from patients with strains of microorganisms consist of individuals that differ not only by the presence of signs of antibiotic resistance, but also on the level of resistance.
In addition, hospital strains of microorganisms consist of individuals that differ in clinically significant signs of aggression and virulence (antilysocyme, hemolytic, proteolytic activity, and others) [Ephtalou, Vli. Mixed infections of burn injury, Kursk, 1998, 92 S.], which is an important pathogenetic importance for the development of any infection.
Without knowledge and consideration of the mechanisms of creation the hospital strains is very difficult etiological diagnosis of pathogens of nosocomial infections. If not to take into account the heterogeneity of microbial populations in the past, evaluation of different methods of identification, proposed by the authors is incorrect.
Secondly, an essential disadvantage of the proposed methods is that during microbiological monitoring of septic infections, the authors diagnosed strains as hospital solely on the basis of studying the results of the comparative evaluation without studying the past against the background of immunomodulators. And it is known that the selection of the most virulent and multidrug-resistant individuals from the population of any strains of pathogens (i.e. the formation of hospital strains and their associations) occurs only through the body immunocompromised patients. Consequently, the main factor influencing the formation of hospital strains and their associations, is the level of immune protection of an organism. Hence conduct research proving hospital nature of the strains and their associations, without the use of immunomodulators in immunocompromising patients is not significantly probative factor.
In connection with this invention was to develop a simulation method of preventing the establishment of hospital strains that can prevent the change of the properties condition the ESD pathogenic microorganisms in the direction of enhancing their virulence and resistance to antibiotics, what is the basis for the formation of hospital strains in the various types of opportunistic septic processes.
The objective of the invention is achieved by the fact that the experimental animals (mice CBA) with burn trauma on the background of fungal and bacterial infections, accompanied by a sharp inhibition of anti-infective protection, three days in a row with an interval of 24 hours between injections intramuscularly Galavit 1 time per day dose mg/mouse and lidocaine intramuscularly 1 time per day at a dose of 8 mg/kg of body weight. Next studied the population structure of pathogens of nosocomial infections on the signs of drug resistance and the major virulence properties.
The method is as follows. The experiments were performed on mice CBA passed a quarantine regime of the vivarium of the Kursk state medical University and had no external signs of any diseases. In the control and experimental group Requirements of the International Committee, set forth in the Convention for the protection of vertebrate animals used for experimental purposes (, Strasbourg, France, 1986) that fully complied with.
It is known that the formation of hospital strains occurs through the body immunocompromised patients. In this regard, to create immunodefi is that in animals we have chosen the model of a thermal burn, because it is known that this condition is always accompanied by the decline of the immune status of the organism. Thermally burns III-B degree area of 30% of the body surface caused essential Rausch-anesthesia by means of a device for applying a metered burn.
Infection of mice were produced by intraperitoneal injection of 0.2 ml of 2 billion mist daily culture of microorganisms (fungi of the genus Candida and Pseudomonas aeruginosa), isolated from patients with burn injury and inflammatory processes. Two days after infection, animals were taken out of their experience using ether anesthesia and sterile conditions removed spleen. Produced blood cultures and suspensions of the spleen on the elective environment (CPH - agar for Pseudomonas bacteria and the environment Saburo - for fungi). A suspension of the obtained cultures were infected the next batch of animals, i.e. produced passages. Just spent 6 passages. After each second passage took the blood on the study and produced the seeding suspension of the spleen to obtain isolated colonies. To study the structure of the population on grounds of drug resistance obtained colonies (at least 100) velvet stamp transferred to plates with meat-peptone agar containing 25 μg/ml of antibiotics: carbenicillin, streptomycin, polymyxin b and kanamycin (for Pseudomonas bacteria); amphotericin b,levorin and nystatin (for fungi of the genus Candida). To study the population structure of the studied strains on the basis of pathogenicity same 100 colonies were seeded on 5% blood agar (hemolytic activity) and on Petri dishes with a collapsed horse serum (proteolytic activity). Cups with crops withstand a day in thermostat at 37°C and took into account the results of drug susceptibility in the presence of growth on media with antibiotics and the presence of hemolysis on cups with blood agar and proteolysis - on cups with rolled serum.
To study the prevention of the formation of hospital strains of the structure of microbial populations was studied on the background of the application of Galavit with lidocaine.
Galavit - domestic immunomodulator, has immunomodulatory, anti-inflammatory and regenerative effects (Khaitov R.M P.M., Pinegin B.V. Immunomodulators: mechanism of action and clinical use // Immunology. - 2003. No. 4. - S-202). Furthermore, of great importance in the early days of the disease with burn injury has a painful shock, which in turn also causes suppression of the immune response. Considering the pathogenesis of burn disease, we recommend the use of immunomodulatory drugs in combination with lidocaine, which not only suppresses the expression of a painful syndrome in burn injuries, but also prevents the post of the managing in the bloodstream of toxic and IMMUNOSUPRESSIVE substances during the development of infection on the background of the burn injury. Significant positive lidocaine is also the fact that lidocaine is not difficult ether, it is slowly metabolized in the body and thus has a prolonged effect.
Due to the fact that during its metabolism in the body does not generate para-aminobenzoic acid, it does not antisulfanilamidnoe actions and can be used in patients receiving antibacterial sulfa drugs (Medmaravis. Drugs // Manual for doctors. - 2001, Vol. 1. - S).
Indicators to examine the status of the level of immune protection were: phagocytic activity of neutrophils in peripheral blood (% of active phagocytes), phagocytic number (the average number fagocitaria microorganisms per one fagozyt), which was determined according to standard techniques. She has also studied phagocytic reserve neutrophils (NEF) and the state of bactericidal systems of neutrophils, which is, as you know, significant indicators in pyo-inflammatory processes of any etiology. NGF was determined by reduction reaction of nitrocine of tetrazole (Waxman M.E. Ter-Minassian, mayansky A.N. The way to assess the functional activity of human neutrophils by the reaction of recovery narasinga of tetrazole // Methodical recommendations. - Kazan. - 1979. - 17 S.). The bactericidal activity of si is the neutrophil - the presence of lysosomal cationic proteins in the cytoplasm of leukocytes (Subic MG Identification of cationic proteins in the cytoplasm of leukocytes using bromthymol blue // Questions of medical chemistry. - 1090. No. 10, T.16.. - S).
Statistical processing of results was performed using the software package "Microsoft Office XP" using student's criterion.
Experimental animals were divided into groups of 25 animals each):
II. Animals with burn trauma, infected by Pseudomonas aeruginosa (P. aeruginosa) is a bacterial infection.
III. Animals with burn trauma. Infected by yeast-like fungi (Candida albicans) is a fungal infection.
IV. Animals with burn trauma, infectious bacterial infection and treated with Galavit in combination with lidocaine;
V. Animals with burn trauma infected with a fungal infection and treated with Galavit in combination with lidocaine.
Infected animals passages cultures, the study of population structure of the studied strains and level of immune protection of an organism, as described above, page 4-6.
Galavit introduced vnutrimyshecnam at a dose of 200 μg/mouse immediately after playback of burn injury 1 times a day for 3 days.
Lidocaine intramuscular injection at a dose of 8 mg/kg of body weight immediately after playing ogogo the second injury 1 times a day for 3 days.
Burns III - degree area of about 30% of the body surface were obtained under light ether anesthesia on devoid of hair and skin back using a device that supports the temperature of the burning surface at 100°C With exposure 8 seconds (WMO, 1985).
We found: animals with burn trauma, complicated both bacterial and fungal infection are greatly reduced all parameters of phagocytosis compared with the control (intact) animals, and there is a high microbial colonization of the spleen. In the same conditions, but after applying calavita with lidocaine phagocytosis indices is significantly higher and even higher than those of the control animals; the splenic index (the number of microorganisms on the body) in treated animals was significantly decreased already in the study after 4 passages, and after 6 passages as bacteria and fungi from the spleen were not inoculated (table 1).
|The effect of immunomodulators on the performance of anti-infective organism defense|
|The studied parameters||Groups of animals|
|The intact||Burn+infection||Treatment with Galavit with lidocaine|
|The indicators in different study periods (passages) M±m (n=25)|
|The activity of phagocytosis||60,8±0,15||60,2±0,17||60,5±0,12||32,7±2,5||30,1±2,7||35,8±2,9X||58,5±1,7||62,3±1,3||68,2±0,8XX|
|The colonization of the spleen (micrel/body)||//||//||//||2.4 x 105||Solid growth||6×107||3×102||Individual coloniesXX||The lack of growth|
|Note: X = p<0,05 - reliability of differences with the data of the control group (intact);|
|XX = p<0,05 - reliability of differences with the data of the control group (burn+infection);|
|// - studies have not been conducted.|
The results of the study structure of the population of microorganisms in the dynamics of infectious process in vivo has shown that in parallel with the improvement of the immune status of the animal organism has been significant changes in the composition of populations septic about what escow. If in the composition of populations obtained from infected animals with burn trauma, experienced a continuous increase in individuals with signs of resistance to antibiotics and signs virulence (signs virulence already to 6 passage populations as Pseudomonas bacteria, and fungi of the genus Candida were statistically significantly (p<0,01) homogeneous, i.e. there is a formation of strains with signs of "hospital" through immunocompromising the body, then after applying calavita with lidocaine (increase immune status), on the contrary, the number of individuals with these characteristics are statistically significantly (p<0,01) was decreased, i.e. the use of immunomodulators promotes reliable elimination of the population of virulent and antibiotic resistant individuals, which leads to superficial pathogens in the body, which is observed from passage 4 of the study (table 2).
|The effect of immunomodulators on the composition of populations of pathogens|
|Learn the signs of||% of individuals in populations (out of 100 colonies)||P|
|the t untreated animals (burn+infection)||from treated animals (Galavit+lidocaine)|
|The indicators in different study periods (passages)|
|Resistance to:||The population of Pseudomonas aeruginosa|
|Resistance to:||The population of Candida albicans|
|Note: the significance of differences with the data of the control group (untreated animals with burn and infection).|
Thus, the obtained results show conclusively that despite the pronounced immunodeficiency in the dynamics of infectious process conditions are created when the original heterogeneous populations of agents of nosocomial infections are generated strains with signs of a hospital. The use of drugs, reducing anti-infective resistance in experimental animals, contributes to the elimination of the composition of microbial populations virulent and antibiotic resistant individuals, which leads to superficial pathogens in the body and 100% survival of animals. There are credible reasons to believe that in incesti conditions improve immune status immunocompromising patients with immunomodulators will prevent the formation of hospital strains of pathogens, will increase the treatment efficiency and reduce the time of their stay in hospital.
The simulation method of preventing the establishment of hospital strains, characterized in that the experimental animals the mice CBA with burn trauma on the background of fungal and bacterial infections, accompanied by a sharp inhibition of anti-infective protection, three days in a row with an interval of 24 h between injections intramuscularly "Galavit" 1 time daily at a dose of 200 μg/mouse and "Lidocaine" intramuscularly 1 time per day at a dose of 8 mg/kg of body weight.
SUBSTANCE: invention may be used in medicine for early diagnosing of stomach cancer. A sensor element is a bacterial cell Helicobacter pylori containing plasmid pHP presented in dwg. 1 with the bacterial gene gfp under control of the inducible stress promotor PflaA.
EFFECT: invention enables fast and reliable wide-range pH measurement.
3 dwg, 2 ex
SUBSTANCE: method of sampling Lactobacillus bacteria to be included in formulations of probiotic preparations provides preparing isolates of said bacteria. The bacteria are sampled by activity of their enzymatic systems responsible for destruction of mycotoxins which are determined by an average geometry of activity of total dehydrogenases, peroxidases and cytochrome P-450-dependent oxidases. The bacteria having the average geometry of enzymatic activity 3.0 standard unit/1 g of nucleic acids and more are sampled.
EFFECT: probiotic preparations of the Lactobacillus bacteria sampled by the presented method; poultry feeding with said preparations combined with the mycotoxin-contaminated forages improves poultry safety, live weight of chicken broilers, gross increment.
3 tbl, 2 ex
SUBSTANCE: method involves isolation, culture and replication of avian embryo stem cells in a complete culture medium containing all growth factors in the presence of an inactivated feeder layer, and added with animal serum; separation of the growth factors, serum and feeder layer; production of the non-adhesive embryo stem cells; proliferation of the produced cells in a suspension in the form of cell balls; infection of said cells with said virus; addition of the second serum-free medium to the cell culture; further culture of the infected cells for virus replication and collection of said virus.
EFFECT: invention is applicable for preparing vaccines and particularly enables producing viral strains which cannot be adequately growth in eggs.
38 cl, 23 dwg, 4 ex
SUBSTANCE: for the purpose of prediction of developing bacterial complication of a staphylococcal aetiology following influenza, the pure culture S. aureus is recovered from nasopharyngeal mucosal microflora to determine hemolytic activity (HA), antilysozyme activity (ALA), then S.aureus is co-incubated in vitro with type B influenza; S.aureus is analysed again for hemolytic and antilysozyme activity, and increasing S.aureus HA by 1 mm and more and/or ALA by 1 mcg/ml and more after co-incubation, a developing bacterial complication is predicted.
EFFECT: invention enables predicting the developing bacterial complication of a staphylococcal aetiology in the patients with influenza that gives the chance to plan and implement the targeted preventive therapeutic interventions reducing a period of staying in hospital.
5 ex, 1 tbl, 2 dwg
SUBSTANCE: estimating a degree of Pseudomonas aeruginosa strain pathogenicity is ensured by taking integral exometabolites of the night culture of Pseudomonas aeruginosa, freezing at minus 18°C for at least 1 hour, thawing and mixing with a rehydrated sensor that is a test culture of E coli lux+. The mixture is kept for 30 min at 20°C, and a degree of inhibition of test culture bioluminescence shows a potential pathogenicity index (PPI) calculated by formula PPI=(Ir-It)/lk·100% wherein Ik is the luminescence intensity of the reference sample, It is the bioluminescence intensity of the test sample. The value PPI≤30% shows a low degree of P. aeruginosa strain pathogenicity; the value PPI varying within 30% to 70% shows a moderate degree of pathogenicity, while the value PPI>70% presents a high degree of P. aeruginosa strain pathogenicity.
EFFECT: unification, cutting time and simplified analysis procedure to high precision.
SUBSTANCE: antibacterial activity of aerobic sporous microorganisms Bacillus subtilis is estimated by test culture inoculation of the whole surface of meat infusion agar in Petri dishes. Zeolite granules of the Hongurinskoye deposit is prepared for 60 min at room temperature and processed with a suspension of bacterial antagonist strains Bacillus subtilis TNP-3-DEP and Bacillus subtilis TNP-5-DEP. They are placed on Petri dishes. The inoculation products are incubated and calculated by bacterial antagonist growth power after 72 h of incubation.
EFFECT: efficiency of determining antibacterial activity of the bacteria.
SUBSTANCE: method involves collecting samples of planktons inhabiting a water body, determining the contamination level through analysis thereof and evaluating analysis results, wherein the contamination level is determined by determining conservative and variable gene sequences of a plankton from the sample, followed by molecular physiological analysis to determine evolutional relationships of the analysed organism with other saprobionts. Analysis results are evaluated as follows: at high (more than or equal to 70%) value of bootstrap cluster support on the conservative and variable gene, including the analysed plankton and resistant saprobionts. The following conclusions are made. When the resistant indicator organisms of xeno- and/or xeno-oligosaprobic water bodies and the analysed plankton merge into a single cluster, the water body is in a safe ecological state and there is no threat of negative human impact. If the resistant indicator organisms of oligo- and/or oligo-mesosaprobic water bodies and the analysed plankton merge into a single cluster, the water body is in an unstable ecological state, is capable of self-restoration and does not need additional nature conservation activities. When the resistant indicator organisms of meso- and/or meso-polysaprobic water bodies and the analysed plankton merge into a single cluster, the water body is in a bad ecological state and needs nature conservation activities. When resistant indicator organisms of polysaprobic water bodies and the analysed plankton merge into a single cluster, there is a local ecological disaster and there is need for urgent remedial measures.
EFFECT: high reliability of biomonitoring results for use without limitation of territory, regardless of the geographical location of the analysed water body.
1 tbl, 2 ex
SUBSTANCE: mice are infected by the oral introduction of a suspension 0.1 ml of a two-day swine Rhodococcus equi, pathogenic of culture containing 5×107 CFU in normal saline 1.0 ml. The infection is followed the oral five-fold introduction of a probiotic preparation 1-5×105-6 CFU in 1.0 ml every 24 hours; in 5 days after the infection, the mice are killed, and dense egg medium are inoculated of the internals suspensions. In 1-2 cultivation days at optimal temperature, but not later than in 5 days, the grown colonies are controlled by acid fast stain. Antagonist activity is evaluated by a growth block index that is a relation of Rhodococcus colony count grown at inoculation of the internals suspensions of the animals prescribed with no probiotic preparations to Rhodococcus colony count grown at inoculation of the internals suspensions of the animals prescribed with the probiotic preparations. The efficacy of the probiotic preparation is concluded if the value exceeds 3.
EFFECT: invention provides fast and nocardioform actinomycete adequate screening of the probiotic preparations in vivo.
15 tbl, 14 ex
SUBSTANCE: method includes measurement of microorganisms test function level in presence and in absence of an analysed sample and calculation of toxicity based on the produced results. Dense samples are exposed to testing (wastes, soils). At the same time testing is carried out without preliminary procedure of producing an aqueous extract of the sample. A test item is a culture of a soil bacillus with dehydrogenase activity, and the test function is its dehydrogenase activity, which is identified using resazurin and recorded using a conventional measurement device for spectrophotometry.
EFFECT: increased efficiency of wastes and soils toxicity identification.
SUBSTANCE: invention represents control strain of Neisseria gonorrhoeae, intended for intra-laboratory control over quality of analysis in carrying out laboratory diagnostics of gonococcal infection by culture method. Strain was isolated from patients with uncomplicated gonococcal infection before beginning their treatment with antimicrobial medications and is deposited in state collection of microorganisms of FSUE " Research institute of standardisation and control of biological preparations named after L.A. Tarasevich Rospotrebnadzor" under No 283. Obtained strain stably preserves its signs and properties and is the first our country reference-strain for intra-laboratory control over research quality in laboratory diagnostics of gonococcal infection.
EFFECT: application of control strain in accordance with the invention will make it possible to increase diagnostics accuracy due to constant comparison of obtained results of diagnostics of analysed cultures with the same signs, which control strain demonstrates.
SUBSTANCE: strain of Sphingobacterium multivorum RNCIM B-10385 is proposed, used to obtain a bacterial fertiliser for tomatoes and cucumbers.
EFFECT: strain Sphingobacterium multivorum RNCIM B-10385 enables to reduce the content of nitrate nitrogen in fruits of cucumbers and tomatoes, while increasing the productivity of a vegetable crop.
1 tbl, 6 ex
SUBSTANCE: Clostridium acetobutylicum strain, the Russian National Collection of Industrial Microorganisms B-10289 is produced by the exposure to UV radiation of a bacterial suspension of the Clostridium acetobutylicum strain, No. 6 that is followed by the anaerobic steady-state selection. The n-butanol yield makes 8.3-14 g/l.
EFFECT: in the various fermentation environments, the strain is characterised by a higher level of n-butanol biosynthesis.
2 tbl, 6 ex
SUBSTANCE: what is described is a new Burkholderia cepscia KM 196 strain carrying nitrosoguanidine (NG) induced mutation in the sequence of opcPl gene responsible for expression of porine protein of molecular weight 36 Da. The strain is produced by two mutagenesis cycles of N-methyl-N'-nitro-N-nitrosoguanidine (NG) of the B.cepacia strain 25416 that is followed by the target recovery of mutant clones in the concentrations of pefloxacin (ofloxacin) and ceftazidime decreasing from the maximum permissible concentration for the initial strain. The clones sensitive to kanamycin, tetracycline, chloramphenicol and deficient in production of major proteins of the external membrane are specified among the mutants with varied sensitivity to cephalosporine and fluoroquinolone antibiotics. The strain 25416 is a version with selected markers of perfloxacine, ofloxacine, ceftazidime, kanamycin, tetracycline, chloramphenicol sensitivity (PfxSOfxSCfzSKanSTetSChiS), deficeint in porine protein OpcPl with Mr 36 kDa and deposited in the State Collection 'Microbe' No. 25416 NSP.
EFFECT: strain shows higher sensitivity to various antibiotics and aims at studying of the molecular-genetic bases of multiple drug resistance in Bcepacia and philogenetically related species of Burcholderia.
2 dwg, 2 tbl, 3 ex
SUBSTANCE: composition contains plastic and EM ceramic, which is first held in a semi-dry state for 24 months and calcined at temperature 1150-1180°C, with content of EM ceramic in the composition of 0.3-0.9 wt %. The EM ceramic is made from clay which is mixed on a preparation made from effective beneficial microorganisms with an additive of rock table salt in ratio 1:1: 1/50, respectively.
EFFECT: improved method.
8 tbl, 2 ex
SUBSTANCE: what is offered is a composition containing inactivated bacteria specified in the strains deposited under registration numbers ATCC No. PTA-3667, ATCC No. PTA-3668, ATCC No. PTA-3669 or their mixture and acceptable adjuvant(-s) and/or excipient(-s). Said vaccine contains min. 107-108 CFU/said bacteria/ml.
EFFECT: poultry immunisation.
22 dwg, 1 tbl, 6 ex
SUBSTANCE: pieces of the affected organs (a biomaterial) are grinded to prepare a suspension. The prepared suspension is filled with a solution containing 4-6% of citric acid and 3% of hydrogen peroxide in the ratio 1:1 to the biomaterial suspension and kept for 30-60 minutes at room temperature to be thereafter processed in a solution containing 4-5% of succinic acid and 3% of hydrogen peroxide in the ratio to the suspension 1:1. It is kept for 30-60 minutes and then neutralised with 5-10% ammonia to pH 7.0. It is followed by decantation of the supernatant, and the Lowenstein-Jensen agar medium is inoculated with the precipitation.
EFFECT: invention allows reducing mycobacteria recovery time.
1 tbl, 1 ex
SUBSTANCE: Bacillus amyloliquefaciens strain, Russian National Collection of Industrial Microorganisms B-10291 is produced by the multistage selection on a medium containing rifampicin that is followed by the selection of the most productive rifampicin-resistant clones. The strain produces alpha amylase of activity 900 GOST units/ml.
EFFECT: strain exhibits a lower level of foaming that allows reducing an amount of an antifoam additive used during fermentation more than in 3 times.
3 tbl, 3 ex
SUBSTANCE: nutrient medium contains solid and liquid phases. The solid phase of the nutrient medium contains a pancreatic digest of fish meal, a hemophilic bacteria growth stimulator, sodium chloride, yeastrel, glucose, agar, 2% potassium tellurite and distilled water in the preset proportions. The liquid phase contains the pancreatic digest of fish meal, enzymatic peptone, sodium chloride, yeastrel, glucose, cattle blood serum and distilled water in the preset proportions.
EFFECT: invention allows higher sensitivity of the medium, germination value and growth rate of diphtheria bacteria.
SUBSTANCE: Clostridium acetobutylicum strain, the Russian National Collection of Industrial Microorganisms B-10290 is produced by the exposure to UV radiation of a bacterial suspension of the Clostridium acetobutylicum strain, the Russian National Collection of Industrial Microorganisms B-4786, that is followed by the anaerobic steady-state selection. The n-butanol yield makes up to 20 g/l on a medium consisting of a rye flour suspension with added molasses and maltose.
EFFECT: in the various fermentation environments, the strain is characterised by a higher level of n-butanol biosynthesis.
2 tbl, 9 ex
SUBSTANCE: invention discloses attenuated bacteria B pertussis, containing the mutant genes ptx and dnt showing no dermonecrotic toxin activity, however having kept an ability to link with an eukaryotic cell receptor. The bacteria under the invention produce a genetically changed immunologically non-toxic toxoidal form of pertussis toxin. The invention concerns the bacterial strain B.pertussis containing said mutant genes which is deposited in the Collections of Strains of Gamaleya Research Institute of Epidemiology and Microbiology, No. 171/331 B.p KS-4. The strain is applicable as a vaccine. The whooping cough agent vaccine contains the living attenuated bacteria or the inactivated attenuated bacteria B.pertussis.
EFFECT: experimental intranasal immunisation in vivo with the vaccine containing the living or inactivated attenuated bacteria Bpertussis under the invention provides the effective and prolonged protection against infection by the virulent strain of the whooping cough agent.
4 cl, 4 dwg, 6 tbl, 5 ex
SUBSTANCE: method of growing Lentinula edodes. Berk myceliuminvolves inoculation of Lentinula edodes. Berk mycelium on a culture medium and selecting primary mycelium. The growth stimulant used is selective light with wavelength 430-480 nm, intensity 70-150 mcmol/cm2·s and exposure time of 1-60 minutes. Lentinula edodes. Berk mycelium is grown at temperature 24-26°C or 10-12 days. With exposure time of 30 minutes, the output of air-dry mass of mycelium is 72 mg.
EFFECT: method increases output of the air-dry mass of mycelium.
2 dwg, 2 ex