Method for determining severity in patients with bronchopulmonary diseases

FIELD: medicine.

SUBSTANCE: invention involves patient's induced sputum sampling, cell substance recovery and preparation for the examination. A cell alteration value is determined by apoptosis evaluation, while the cell substance is additionally examined for a cell necrosis alteration value. The apoptosis and necrosis values are determined in two or more examinations every 1-3 days. The derived values are expressed in the form of a necrosis index (NI) and an apoptosis index (AI) to calculate a cell alteration index (CAI) by formula: CAI=NI/AI, wherein NI is the necrosis index; AI is the apoptosis index. A severe clinical course of the bronchopulmonary disease is stated by the CAI values within 0.5 to 1.0. A moderate degree of severity is shown by the CAI values falling within the range 1.0 to 1.3. And a mild degree is indicated by the CAI values in the range 1.3 to 1.7. The clinical effectiveness of the bronchopulmonary disease can be determined by comparing the initial CAI values and the CAI value during treatment, and if observing increasing the CAI values, the improvement is diagnosed.

EFFECT: using the method enables higher accuracy of determination with providing more simple and fast method.

2 ex, 2 tbl


The invention relates to medicine, namely to diagnosis, and can be used to determine the severity of bronchopulmonary diseases.

The development of inflammation in bronchopulmonary diseases depends on the duration of the recruitment of cells from the bloodstream into the lining of the bronchi and activation of these cells. A Central role in the inflammation site is given to the mechanisms of regulation of cell viability. There are two forms of cell death - necrosis and apoptosis. The first is cell death caused by exposure to harmful factors leading to the violation of the integrity of cell membranes, resulting in cell literoitca and its contents poured into the intercellular space. Cell necrosis is usually accompanied by inflammation. Apoptosis is a special type of programmed cell death by dividing them into parts ("apoptotic Taurus"), which are then phagocytized by neighboring cells of different types that does not lead to the development of inflammation. These forms of damage or alteration of the cells are of great importance in homeostasis and pathological conditions of the body. It is considered that if the cells die due to necrosis, the possibility of pharmacological intervention may not give a positive result, even if it is only the initial stage is uridine. However, according to Professor V.S. Novikov (Programmed cell death / Usenbekov. - St. Petersburg: Nauka, 1996), therapeutic effectiveness in the inhibition of realization of cellular collapse (apoptosis) can be achieved with the early and comprehensive application of pharmacological agents.

Known way to determine the severity of bronchopulmonary diseases - bronchial asthma (Bespalova I.D., Crnogorac GA, V.T. Volkov EN 2277710 C1 from 2006.06.10), consisting in the determination of inflammatory markers in biological fluids, using the definition of the reaction of a skin window. Received strokes, after 12 and 24 hours, fixed with methanol and stained by Bernhard. Determine the number of neutrophils and macrophages, counting from 100 to 500 cells in each smear and the percentage of neutrophils 57,78±4,40 and 32,35±3,61 after 12 and 24 hours respectively define first degree of inflammatory activity, while the percentage of neutrophils 62,56±5,50 12 hours, and 40,88±6,22 24 hours determine the second degree of activity of inflammation.

The disadvantage of this method is that it is traumatic, because as inflammation markers in biological fluids using clarificatory the skin of patients, time-consuming for the researcher and long.

There is a method of assessment effectivelyintegratedinto therapy Chlamydophila pneumoniae in patients with bronchial asthma (Fedorov G., Grigoriev, V.N., Petushkova so-CALLED. EN 2230319 C1 from 2004.06.10). At the same time as inflammation markers in biological fluids using peripheral blood of patients, in which method an indirect immunofluorescence assay to determine subpopulation structure of lymphocytes. When CD4+lymphocytes of less than 36,67% and D95 lymphocytes of less than 57,95% 3-4 weeks after treatment antibiotic therapy aimed at the eradication of the pathogen, it is considered effective.

The disadvantages of this method are:

the lack of data about the state of local immunity of patients;

the subjectivity of the received data, as the results will largely depend on the skill of the person fulfilling it;

- high costs in connection with the use, to identify subpopulations of cells monoclonal antibodies.

The closest to our proposed method is the evaluation of apoptosis of neutrophils in patients with sepsis, accompanied by acute respiratory distress syndrome (Fialkow L., Filho, L.F., Bozzetti M.C., Milani A.R., Filho E.M.R., Ladniuk R., Pierozan P., de Moura R., Prollal J.C., E. Vachon, G.P. Downey Neutrophil apoptosis: a marker of disease severity in sepsis and sepsis-induced acute respiratory distress syndrome Critical Care. - 2006, 10(6):R155). With this method neutrophils isolated from the peripheral blood of patients by adding dextran and use specific gradient in density is of astora, after sedimentation of the cells washed twice from these solutions, the concentration is brought to 1×106cells in 1 ml RPMI medium containing 10% fetal calf serum, 2 mM L-glutamine, 100 mg/ml penicillin, 100 mg/ml streptomycin, after incubation for 24 h, colouring neutrophils by Giemsa and assesses the status of the cells for the identification of changes to the kernel 500 cells (chromatin condensation and reduction of nuclear structure), which is expressed in percentages. Thus the dependence of the degree of apoptosis of neutrophils of patients with sepsis from the severity of the disease.

The disadvantage of this method is that this method captures only system disorders in patients, and in the appointment of therapy in patients with bronchopulmonary diseases, it is necessary to investigate the condition of the cells not only systemic blood flow, but also directly target organ (lung). It is known that in the target organ cells reach the last stage of functional maturation, which, ultimately, leads to the initiation of the process of their death. In addition to the disadvantages of this method is the long period of incubation of the cells for which use additional reagents and determination of apoptotic activity only neutrophils, without regard to other cells.

adaca of the proposed technical solution is to obtain high precision data, simplification and acceleration of the method.

The problem is solved in that in the method for the diagnosis of disease severity in patients with bronchopulmonary diseases, including selection of subject matter, the allocation of a cellular substance, preparation to study, determine the magnitude of alteration of cells by assessing their apoptosis according to the invention as the study material used induced sputum (IM) patient, in the cellular substance additionally determine the amount of alteration of cells by necrosis, indicators of apoptosis and necrosis is determined at two or more surveys with an interval of 1-3 days, the resulting performance is expressed as the apoptotic index (AI) and index of necrosis (JN), calculate the index of alteration of the cell (FCC) by the formula:

IAC=IN/AI, where

IN the index of necrosis;

AI - apoptotic index;

the degree of severity of the disease in patients determined on the basis of the values of IAC and diagnose severe currents bronchopulmonary disease with indicators IAK from 05 to 1.0, the average from more than 1.0 to 1.3 and a mild degree of flow at rates ranging from more than 1.3 to 1.7

The essence of the method is illustrated by the following sequence of operations:

As the material for research using induced sputum (MI) patients with BD who holehouse diseases. THEY receive after inhalation 3-5% sterile hypertonic saline using compression nebulizer "Omron CX-3 (Japan). Then IM using sterile disposable syringe (5 or 10 ml) is transferred into a test tube. For dispersion and homogenization of sputum using 0.1% solution of dithiothreitol, which is added to the sputum in a 1:1 ratio. The mixture is then shaken for 10 minutes and filtered through nylon gauze. Filtered THEM centrifuged at 1200 rpm for 10 minutes, then the supernatant is drained, and a suspension of the cells washed 3 times in Hanks solution without phenol red by centrifugation at 1200 rpm for 10 minutes.

Sputum examination shall be held not later than 3 hours after receipt of material throughout this time the samples are stored at a temperature of +4°C.

To determine necrosis cell suspension added at the rate of 1:2 solution of 0.1% Triton X-100, 0.08 N HCl, M NaCl, gently mix and leave for 15 seconds, then bring the rate of 1:2 cold solution of dye acridine orange (5 mg/ml, on the basis of citrate buffer (pH 6.0)containing 1 mM EDTA-Na, M NaCl, 0.2M NaPO4, 0.1M. After coloring within 5-10 minutes preparing medicines and immediately analyzed under fluorescent microscope.

To determine apoptosis, after mixing the cell suspension with a solution of Triton (composition of the solution specified above), cells fixed with 4% paraformaldehyde on the basis of distilled water containing 1% of cacodylate sodium and 1% sodium chloride, and then stained with a specific dye Hoechst 33342 (5 mM) for detection of apoptosis for 15 min and analyzed under fluorescent microscope.

Damage (alteration) cells induced sputum appreciate by viewing drugs under fluorescent microscope with exciting filters FS, SS, ES and locking filter type LGL=18+LGL=19 using a water immersion lens. The results are obtained on the basis of the evaluation of color, intensity and morphology of the fluorescent cells. In the case of alteration of cells by necrosis count 100 cells, of which define % yellow cells, including dye is acridine orange, and expressed as an index of necrosis (JN). Apoptotic cell activity is expressed as the apoptotic index (AI) - % of green cells, including dye Hoechst 33342, and with the presence of morphological signs of apoptosis - modified nuclei, consisting of 3 or more items, and reduced cytoplasm.

Calculate the index of alteration of the cell (FCC) by the formula: IAC=IN/AI, where JN is an indicator of necrosis; AI - apoptotic index.

Compare the index of alteration of the cells of patients in exacerbation with indicator IAK healthy individuals (counter is eh). Reduction IAK phagocytes THEM in a patient, compared with values in healthy donors, indicates a violation of local protection in the target organ. Diagnosed with severe currents bronchopulmonary disease with indicators IAK from 05 to 1.0, the average from more than 1.0 to 1.3 and a mild degree of flow at rates ranging from more than 1.3 to 1.7

The basis for determining the effectiveness of the proposed method of evaluation of bronchopulmonary diseases was based on the results of the study of induced sputum of patients with community-acquired pneumonia (EP), bronchial asthma (BA) mild, moderate and severe in the process of inpatient treatment in the pulmonary Department of the General hospital of the Pacific fleet, Vladivostok) and therapeutic Department DOMC (Vladivostok). Clinical diagnosis of bronchopulmonary disease was established on the basis of common criteria. Assessment of the severity of patients at the time of admission was carried out according to the criteria proposed Lignorance in 1996 (A. Okorokov. Diagnosis of respiratory diseases / Anecortave. - M: Honey. lit., 2000. - 448 C.). The study included patients with mild illness - 60 (38.7 per cent), with medium-heavy - 48 (37.4 per cent) and heavy 22 (23.9 percent). The control group consisted of 20 healthy persons with no history of chronic bronchopulmonary pathology is, not suffering from an acute illness within 6 months. This IAK in healthy individuals is equal to 2.0.

A study of indicators of alteration of cellular factors of local immunity (MI) patients with bronchopulmonary disease has made it possible to identify a distinct change their values compared with those for healthy persons. the lowest value IAK was noted in the period of exacerbation in patients with severe EAP (2.7 times) and BA (2.6 times). This marked a clear difference values IAK depending on the severity of the disease, the more severe is the disease, the lower the IAK. The lowest values IAK was noted in patients EAP that pointed to the failure of local factors protect the lungs in this disease.

Examples of specific performance.

Example 1

Patients with a diagnosis of community-acquired pneumonia (EP) remained conservative inpatient treatment pulmonary Department of the General hospital of the Pacific fleet. The EAP was diagnosed with a combination of newly arising infiltrative changes on the radiograph of the lungs and the following clinical signs (Torres, 1997): a) cough b) purulent or Muco-purulent sputum, rales on auscultation or signs of seal lung tissue; d) dyspnea or tachypnea; d) peripheral blood leukocytes >10000 or <4500 m the or > 10% band neutrophils. Considering the fact that the patients included in the study were of the same sex, age and had no comorbidities, the severity of the EAP was estimated on the basis of clinical manifestations according to the criteria developed by Butler LI (1996). Patients the study MI by the proposed method. The data presented in table 1.

Table 1
Indicators of alteration of cells induced sputum (PAC) in patients with RR
PAKEAP first severity n=26VP second severity n=18EAP third degree n=10Control group, n=20
IN %96±6,859±3,849±3,545,2±2,4
AI, %70,01±6,854±4,666,7±5,222,5±1,6
JAMES1,41,10,7The values of IAC, is presented in the table, in the period of exacerbation, patients EAP can be divided according to the severity of the disease. Patients of the first (easy) degree EAP is IAK amounted to 1.4, the second (middle) of 1.1, whereas a third of patients (severe) disease 0,7.

Example # 2

Patients with a diagnosis of bronchial asthma (BA) of various degrees of severity of the disease was on the conservative inpatient treatment therapeutic Department DOMZ. For a diagnosis of ad, determine severity were used recommendations adapted to the Russian conditions of consensus (GINA 2002), assessment of the clinical course BA was performed according to the change of day and night symptoms, measured in points on a scale from 0 to 5, frequency and duration of exacerbations of the disease, indicators of external respiration functions. Patients the study MI by the proposed method. The data presented in table 2.

Table 2
Indicators of alteration of cells induced sputum (PAC) in patients with BA
PAKBA, mild disease severity n=16BA, the average degree t is tin disease n=14 BA, severe degree of severity of the disease (n=8Control group, n=20
IN %42,3±2,444,6±3,439,3±1,645,2±2,4
AI, %28,8±1,837,3±1,843,7±3,422,5±1,6

Thus, the value of IAC in the period of exacerbation with bronchial asthma can be classified according to the severity of the disease. Thus, in patients with mild disease BA is IAK amounted to 1.5, the average is 1.2, and the heavy - 0,9.

The way to diagnose the severity of the disease patients with bronchopulmonary diseases, including selection of subject matter, the allocation of a cellular substance, preparation to study, determine the magnitude of alteration of cells by assessing their apoptosis, characterized in that as the study material used induced sputum of a sick person, in the cellular substance additionally determine the amount of alteration of cells by necrosis, however, is ately apoptosis and necrosis is determined at two or more surveys with an interval of 1-3 days, the indicators expressed as an index of necrosis (JN) and apoptotic index (AI) and calculate the index of alteration of the cell (FCC) by the formula
the degree of severity of the disease in patients determined based on the value of IAC and diagnose severe currents bronchopulmonary disease with indicators IAK from 0.5 to 1.0, the average from more than 1.0 to 1.3 and a mild degree of flow at rates ranging from more than 1.3 to 1.7.


Same patents:

FIELD: chemistry.

SUBSTANCE: biological object is treated twice for 30 minutes with ethyl acetate; ethyl acetate extracts are combined; ethyl acetate is evaporated; the residue is dissolved in hexane and extracted with a buffer solution with pH 12-13; aqueous-alkaline extracts are separated and acidified to pH 2-3, saturated with sodium sulphate, extracted with diethyl ether; the ether extract is separated, dehydrated, evaporated at 18-22°C in an air current and then in a nitrogen current until complete removal of the solvent; the residue is dissolved in a mixture of solvents, chromatographed in a macrocolumn with silica gel L 40/100µ using a mobile phase; the eluate fractions containing the analysed substance are combined; the eluent is evaporated at 18-22°C in an air current and then in a nitrogen current until complete removal of the solvent; the residue is dissolved in hexane and determination is carried out using a chromato-mass-spectrometric method using a capillary column with length of 30 m and inner diameter of 0.25 mm, which contains a stationary phase, using a helium carrier-gas fed at a rate of 1 ml per minute, and a mass-selective detector operating in electron impact mode; temperature of the injector is 280°C, temperature of the interface is 260°C, temperature of the quadropole is - 150°C, temperature of the ion source is 230°C; intensity of the signal caused by charged particles formed during bombardment with a 70 eV electron beam of the analysed substance, which comes out the capillary column and falls into the ion source, is recorded; the mass spectrum on the full ion current is recorded and the amount of 2-methoxy-4-allylhydroxybenzene is calculated from the area of the chromatographic peak; wherein after evaporation of ethyl acetate, the residue is repeatedly treated with acetone, acetone extracts are combined, evaporated first in an air current at temperature 18-22°C and then in a nitrogen current until complete removal of the solvent; before chromatography in the macrocolumn, the residue is dissolved in a mixture of hexane-diethyl ether solvents, taken in volume ratio 6:4; the stationary phase for chromatography in the macrocolumn is a mixture of hexane-diethyl ether solvents in volume ratio 6:4; during chromato-mass-spectrometric determination, the stationary phase of the capillary column is (5%-phenyl)-methylpolysiloxane, the initial temperature of the column is 50°C, temperature is programmed from 50°C to 200°C at a rate of 10°C per minute.

EFFECT: higher sensifility of the analysis.

2 ex, 3 tbl

FIELD: medicine.

SUBSTANCE: histological sections of internal organs are examined under a microscope; the histological examination is preceded by preparing a solution of diazotised aniline colour for staining the histological sections to detect indole, phenol, skatole; the histological section is stained and microphotographed; digital microphotos of the histological sections are processed on a computer. An average degree of staining with the diazotised aniline colour is estimated by the RGB colour grade system, and if observing the degree of staining within 250 to 100 RGB colour grade units, a mild severity of endogenous intoxication is diagnosed; the degree of staining varying from 100 to 50 RGB colour grade units shows a moderate severity of endogenous intoxication, while severe endogenous intoxication is indicated by the degree of staining being 50 to 0 RGB colour grade units.

EFFECT: using the technique enables higher toxicity and specificity of post-mortem diagnosis of endogenous intoxication based on the immediate detection of toxic metabolites collected in the internal organs.

1 tbl

FIELD: medicine.

SUBSTANCE: method for keeping viable blood cells for purposes of chemoluminescence analysis involves blood sampling and preserving with the blood sampled in disposable plastic test tubes and Faglucide® used as a haemopreserving agent in the ratio 1:6; the prepared blood is kept at temperature 2 to 6°C.

EFFECT: prolonged period of keeping viable leukocytes for purposes of chemoluminescence analysis, maintained the morphofunctional integrity and the rheological blood properties in cattle for 168 hours and the pH value at a physiological level that enables blood transportation for diagnostic researches from distant regions to laboratories.

2 tbl

FIELD: medicine.

SUBSTANCE: invention refers to medicine, namely cardiology, and concerns prediction of the developing life-threatening ventricular arrhythmias in the patients with no structural cardiac changes. That is ensured by determining a blood serum interleukin 6 level by a quantitative method, and the value > 14.9 pg/ml enables predicting the developing life-threatening ventricular arrhythmias in the given category of patients.

EFFECT: method provides specifying the indications to well-timed antiarrhythmic therapy.

3 ex

FIELD: medicine.

SUBSTANCE: invention relates to medicine, namely to pediatrics, and can be used for selection of drug therapy of atherogenic dyslipidemia in children and teenagers with abdominal-visceral obesity. For this purpose index of body weight (IBW) is determined. By results of blood serum lipidogram calculation of atherogenity index (AI) is performed. If IBW is higher than 90-th percentile set for said age group, and AI level is from 2 to 4 including anthropometric parameter - sagittal diameter of abdomen (SDA) is additionally measured. If SDA index equals or is higher than 24.0 cm, state is estimated as requiring drug therapy with chophytol.

EFFECT: method ensures increase of drug therapy efficiency and reduction of risk of early development of atherosclerosis in said group of patients due to possibility of fast and objective determination of degree of expression of risk of early atherosclerosis development, individualisation of indications for drug therapy and its early administration.

1 tbl, 2 ex

FIELD: measurement equipment.

SUBSTANCE: invention refers to measurement equipment and can be used in medicine for various diagnostic purposes. System of biosensors determining the concentration of investigated substance as per output signal generated with oxidation-reduction reaction of the investigated substance is proposed. System of biosensors introduces the correction to relationship in order to determine concentrations of the investigated substance as per output signals at one and the same temperature for determination of concentrations of the investigated substance as per output signals at the other temperature. Relationship with correction to temperature between concentrations of the investigated substance and output signals at reference temperature can be used for determination of concentrations of the investigated substance as per output signals at the specimen temperature.

EFFECT: improving the accuracy of determination of the investigated substance concentration.

29 cl, 12 dwg

FIELD: medicine.

SUBSTANCE: invention relates to oncology, immunology, laboratory diagnostics and is intended for prediction of dissemination of tumour process in patients with stomach cancer. Absolute content of monocytes and concentration of tumour necrosis factor alpha (TNF-α) are determined in peripheral venous blood of examined people. If content of monocytes is lower than 0.35×109 cell/l and TNF-α is higher than 55 pg/ml, high probability of dissemination is predicted.

EFFECT: method makes it possible to reduce time and simplify methods of prediction, as it makes it possible to use materials, reagents and methods of analysis, widely used in standard clinical-laboratory practice.

3 ex

FIELD: medicine.

SUBSTANCE: endothelial dysfunction is diagnosed if observing the twofold increase of a rat's blood glucose level. The technique involves determining antioxidant system values, a concentration of total NO metabolites, eNOS activity, a micro- and macrohaemodynamic status, a MDA concentration and Na+ K+ ATP-ase activity in myocardial and hepatic cell membranes. The values variations enables stating the presence of endothelial dysfunction in alloxan diabetes. Such dysfunction is corrected by the subcutaneous introduction of the preparation Ubiquinone (Coenzyme Q10) 0.11 mcl/100 g of animal's weight once a day for 30 days.

EFFECT: more accurate and reliable diagnosis of the vascular disorders accompanying said type of diabetes, and effective correction of such disorders also provides reproducibility, ease, availability, safety and low cost of performing the experiment.

2 cl, 2 tbl, 6 dwg, 1 ex

FIELD: medicine.

SUBSTANCE: diagnostic technique for syngeneic grafted myeloblastic leukaemia in AKR/JY mice involves determining a blood plasma ceruloplasmin level; if observing the values more the 152.1 mg/l, liver weight more than 1.7 g and a liver myeloblast content more than 25%, developing syngeneic grafted myeloblastic leukaemia in AKR/JY mice is diagnosed.

EFFECT: use of the declared method enables higher accuracy and simplified diagnosing the syngeneic primary myeloblastic leukaemia in mice.

1 ex, 1 tbl

FIELD: medicine.

SUBSTANCE: at the moment of admission to hospital, a patient is examined for the presence of acute renal failure, hemorrhagic syndrome, meningitis, jaundice, myocarditis, leucocytosis that is followed by calculation of linear discriminant functions by formula: "ЛДФ1"=-34.12+7.98*X1+7.95*X2+4.27*X3+6.66*X4+12.76X5+0.39*X6 "ЛДФ2"=-57.45+13.77*X1+10.86*X2+5.26*X3+8.88*X4+14.18*X5+0.45*X6, wherein X1=1 in the presence of renal failure, X1=2 in the absence thereof; X2=1 in the presence of hemorrhagic syndrome, X2=2 in the absence thereof; X3=1 in the presence of meningitis, X3=2 in the absence thereof; X4=1 in the presence of jaundice, X4-2 in the absence thereof; X5=1 in the presence of myocarditis, X5=2 in the absence thereof; X6=1 in the presence of leucocytosis, X6=2 in the absence thereof. Observing "ЛДФ1">"ЛДФ2" enables predicting severe leptospirosis, while "ЛДФ1"<"ЛДФ2" shows moderate severity.

EFFECT: use of the method enables higher prediction accuracy of the clinical course of the disease and its objectivity that provides specifying differentiated approaches to therapy of leptospirosis, improving a therapeutic effect.

6 tbl, 3 ex

FIELD: medicine, psychiatry.

SUBSTANCE: one should isolate DNA out of lymphocytes of peripheral venous blood, then due to the method of polymerase chain reaction of DNA synthesis one should amplify the fragments of hSERT locus of serotonin carrier gene and at detecting genotype 12/10 one should predict the risk for the development of hallucino-delirious forms of psychoses of cerebro-atherosclerotic genesis.

EFFECT: more objective prediction of disease development.

3 ex

FIELD: medicine, urology.

SUBSTANCE: one should conduct subcutaneous prevocational tuberculin test and, additionally, both before the test and 48 h later it is necessary to perform the mapping of prostatic vessels and at decreased values of hemodynamics one should diagnose tuberculosis. The information obtained should be documented due to printing dopplerograms.

EFFECT: more reliable and objective information.

1 ex, 1 tbl

FIELD: molecular biology.

SUBSTANCE: the suggested innovation deals with the fact that nucleic acids should be isolated directly out of the sample without pipetting stage but with the help of interconnected reservoirs being prepared beforehand. The above-mentioned vessels should be applied either separately or being interconnected according to standard microtitrating format. The sample should be mixed with a lyzing buffer and nucleic acids are bound with matrix in closed system including, at least, two interconnected reservoirs. Forced movement of sample's mixture and buffer back and forth from one reservoir into another one for several times through narrow passage provides their thorough intermixing. The method provides quick and safe isolation of nucleic acids.

EFFECT: higher efficiency.

44 cl, 4 dwg, 1 ex

FIELD: medicine, phthisiology, microbiology.

SUBSTANCE: diagnostic material is poured preliminary with chlorohexidine bigluconium solution, homogenized, kept at room temperature for 10-12 h and centrifuged. Precipitate is poured with Shkolnikova's liquid medium, incubated at 37oC for 3 days, supernatant part of Shkolnokova's medium is removed, fresh Shkolnikova's medium is added, and precipitate is stirred and inoculated on the dense cellular egg media. Sensitivity of the strain is determined in 3 weeks by the presence of growth in the control tube only. Invention provides enhancing precision and reducing time for assay. Invention can be used in assay for medicinal sensitivity of tuberculosis mycobacterium.

EFFECT: improved assay method.

3 ex

FIELD: medicine, biotechnology, pharmacy.

SUBSTANCE: invention relates to agents used for treatment of pathological states associated with disorder of synthesis of neuromediating substances. Method involves the development of pharmaceutical composition and a method for it preparing. Pharmaceutical composition represents subcellular synaptosomal fractions: synaptic membranes, "light" synaptosomes and "heavy" synaptosomes prepared from gray matter of cerebral hemispheres from experimental animals based on the goal-seeking modification of humoral mediators of nerve endings transformed to synaptosomes in development and regression of malignant processes. The composition provides inhibiting the growth of tumor cells, to elevate span-life of patients with ascite Ehrlich's sarcoma, breast adenocarcinoma Ca-755, Wolker's carcinosarcoma-256.

EFFECT: valuable medicinal and anti-tumor properties of composition.

12 cl, 3 tbl, 3 ex

FIELD: medicine.

SUBSTANCE: method involves carrying out microscopic examination of blood serum samples taken from femoral vein and cubital vein. Femoral vein sample is taken on injured side. The examination is carried out before and after treatment. The blood serum samples are placed on fat-free glass slide in the amount of 0.01-0.02 ml as drops, dried at 18-30°C for 18-24 h. The set of pathological symptoms becoming larger or not changed after the treatment in comparison to sample taken before treatment, and morphological picture of samples under comparison taken from the cubital vein showing no changes or being changed to worse, the treatment is considered to be effective.

EFFECT: enabled medicamentous treatment evaluation in course of treatment to allow the treatment mode to be changed in due time; avoided surgical intervention (amputation); retained active life-style of aged patients.

4 dwg

FIELD: medicine, clinical toxicology.

SUBSTANCE: at patient's hospitalization one should gather the data of clinical and laboratory values: on the type of chemical substance, patient's age, data of clinical survey and laboratory values: body temperature, the presence or absence of dysphonia, oliguria being below 30 ml/h, hemoglobinuria, erythrocytic hemolysis, exotoxic shock, glucose level in blood, fibrinogen and creatinine concentration in blood serum, general bilirubin, prothrombin index (PTI), Ph-plasma, the state of blood clotting system. The state of every sign should be evaluated in points to be then summed up and at exceeding the sum of points being above "+20" one should predict unfavorable result. At the sum of "-13" prediction should be stated upon as favorable and at "-13" up to "+20" - prediction is considered to be doubtful.

EFFECT: higher accuracy of prediction.

2 ex, 3 tbl

FIELD: medicine, juvenile clinical nephrology.

SUBSTANCE: disease duration in case of obstructive pyelonephritis should be detected by two ways: either by detecting the value of NADPH-diaphorase activity, as the marker of nitroxide synthase activity in different renal department and comparing it to established norm, or by detecting clinico-laboratory values, such as: hemoglobin, leukocytes, eosinophils, urea, beta-lipoproteides, lymphocytes, neutrophils, the level of glomerular filtration, that of canalicular reabsorption, urinary specific weight, daily excretion of oxalates, arterial pressure, and estimating their deviation against average statistical values by taking into account a child's age.

EFFECT: higher efficiency of detection.

7 dwg, 1 ex, 6 tbl

FIELD: medicine, urology.

SUBSTANCE: the present innovation deals with differential diagnostics of prostatic cancer and other prostatic diseases at the stage of primary inspection. The method includes the detection of PCA and calculation of probability coefficient for prostatic cancer (PCC) by the following formula: where e - the foundation of natural logarithm (e=2.718…), PCA - the level of total blood PCA in ng/ml, V - patient's age in years. At PCC value being above 0.2 one should diagnose prostatic cancer and to establish final diagnosis one should perform polyfocal prostatic biopsy. The method enables to increase accuracy of diagnostics at decreased number of unjustified prostatic biopsies.

EFFECT: higher efficiency of diagnostics.

2 ex

FIELD: medicine, biology.

SUBSTANCE: invention relates to nutrient medium used for accumulation of cells for the following cytological and/or immunocytochemical analysis carrying out. Invention relates to medium containing salts NaCl, KCl, anhydrous CaCl2, MgSO4 x 6 H2O, MgCl2 x 6 H2O, Na2HPO4 x 2 H2O, KHPO4, NaHCO3, and also glucose and Henx's solution, 10% albumin solution and polyglucin taken in the ratio 1:1:1. Invention provides enhancing the preservation of cells.

EFFECT: improved an valuable properties of nutrient medium.

3 ex