Method of processing animal or human biological tissue, eg swine or bovine pericardium or human cadaver mitral valves and properly processed biological tissue
SUBSTANCE: group of inventions refers to medicine. The group of inventions consists of two objects: a method of processing and a biological tissue. The method is characterised by taking the biological tissue stabilised by glutaric dialdehyde and presented by both animal and human biological tissue, e.g. swine or bovine pericardium or human cadaver mitral valves wherein physical plasma processing, particularly of collagen tissue is performed.
EFFECT: group of inventions provides higher biocompatibility of biological tissue, cell colonisation and prolonged service life.
The invention relates to a method for processing biological tissue of animal or human origin, such as heart valves, pig or heart valves from bovine pericardium or heart valves of the human body and accordingly treated biological tissue.
Regarding the background of the invention it should be noted that the methods work by using pre-processed in different ways biological tissues of animal origin as collagen matrices, in particular, tissue engineering, are of great importance in various surgical areas for future improved therapies. The relevant fields of application it should be noted in cardiovascular surgery, but Orthopaedics and neurosurgery as well as possible applications.
When applying collagen matrix in cardiovascular surgery should be provided with good blood compatibility and mechanical strength. As an example in this connection we should mention heart valves of animal origin, such as valves, pig or valves from bovine pericardium, as well as biological implants vessels with small diameters and pumping cavity biological or mechanical injection pumps blood. When orthopedic surgical treatment is x special interest are the strong collagen matrix to replace cartilage, ligaments and tendons. Finally, in neurosurgery collagen tissue for closure of the skull, for example, after tumor operations, should also be considered as the scope of this invention.
Special problems underlying the invention should be clear from the example of replacement heart valves. So, when replacing heart valves, implanted in more than 200,000 patients per year worldwide, approximately 50% made of artificial mechanical heart valves, and 50% of the biological implants based on the heart valves of pigs and valves from bovine pericardium. When the implantation of mechanical heart valves in the aftercare required the introduction of drugs that prevent blood clotting to avoid embolism occurring in prosthetics. Thus, patients receiving such assistance, be "suffering from artificial hemophilia".
The difficulty with biological heart valves animal is that to achieve long-term stability, strength they should be treated with glutaraldehyde. Due to the presence of free aldehyde groups derived from glutaric dialdehyde, biological heart valves essentially have a toxic effect and are therefore unable to form colonies with to Adami. The formation of cell colonies should make these bioclean reliable for much longer. However, before the formation of cell colonies should be detoxification. Medical studies that were used binders available aldehyde groups, in this context known from the prior art. In this regard, provides links to the following literature sources: Gott JP, Chih P., Dorsey L., Jay J.L., Jett G.K., Schoen F.J., Girardot J. M., R. A. Guyton "Calcification of porcine valves: a successful new method of antimineralisation" ("Calcification (calcification) of the valves of pigs: a successful new way of antimonarchical") in Ann. Thorac. Surg. 1992; 53:207-216; M. Jones, Eidbo E.E., Hilbert S.L" Ferrans V.J., Clarck R.E. "Anticalcification treatments of bioprosthetic heart valves: in vivo studies in sheep" ("Anticarcinoma processing Bioprocessing heart valves: studies in sheep in vivo"in J. Cardiovasc. Surg. 1989; 4: 69-73; Grabenwõger M., J. Sider, Fitzal f, Zelenka C., U. Windberger, Grimm M. "Impact of glutaraldehyde on calcification of pericardial bioprosthetic heart valve material" ("the Effect of glutaraldehyde on the calcification of pericardial bioprotective material heart valves") in Ann. Thorac. Surg. 1996; 62:772-7; and, finally, C.L. Webb, Benedict J.J., Schoen FJ., Linden J.A., R.J. Levy "Inhibition of bioprosthetic valve calcification with aminodiphosphonate covalently bound material to residual aldehyde groups" ("Inhibition of calcification Bioprocessing valve by iminodiethanol, covalently bound substances with residual aldehyde groups") in Ann. Thorac. Surg. 988; 46:309-16.
In addition, research was conducted to achieve detoxification using citric acid. It was partly successful, as described by H. Gulbins, Goldemund A., Anderson I., U. Haas, Uhlig, A., Meiser C., Reichart B. "Preseeding with autologous fibroblasts improves endothelialisation of glutaraldehyde-fixed porcine aortic valves" ("Pre-seeding with autologous fibroblasts improves endothelialization linked with glutaraldehyde aortic valve of a pig") in J. Thorac. Cardiovasc. Surg. 2003; 125:592-601.
Achieved degree of detoxification in this case was only 20% to 30%.
When appropriate detoxification tissue associated with glutaraldehyde, and the corresponding ability to perceive the outer layer of the body's own tissue after implantation, the so-called "endothelialization allows you to reach for heart valve so that it continued to function in life without the introduction of drugs for inhibition of blood coagulation.
Another embodiment of the present invention are implants vessels of small diameter. Implants of this type are now usually made of plastic material, such as PTFE (polytetrafluoroethylene) or PET (polyethylene terephthalate). They have a relatively high rate of clogging, particularly when used as a vascular replacement for p is referencesa vessels of the legs, coronary shunting and peripheral dialysis shunts. The consequences of blockage of blood vessels in these areas prosthetics are severe, including a leg amputation, myocardial infarction, leading to death, or the need to repair shunt. There is also biological tissue of the small diameter of animal origin, linked with glutaraldehyde, in the form of a pig, bovine or goat donor vessels, can lead to a significant improvement in the case, if the fabric of this species can undergo detoxification and endothelialization, or be endothelialization in the blood stream. When applying muscle pumps you can also avoid thromboembolic complications, if the components that come into contact with blood, will be able to increase biocompatibility, for example, through appropriate measures detoxification.
Other applications of such biological tissue in the form of collagen matrices of animal origin can be durable and biocompatible associated with glutaraldehyde and subjected to detoxification of biological tissue for the treatment of osteoarthritis of the hip joint, knee joint and ankle joint. In addition, the application for closure of the skull after trauma or tumor operations using appropriately treated with glutaraldehyde, for example, is by detoxifying bovine pericardium as cerebrosides application may be exactly the same as applied, for example, in thoracic surgery to replace the chest wall or diaphragm, abdominal cavity surgery for replacement of the abdominal wall or ENT (ear, nose, throat) area to replace the eardrum.
Given the difficulties described in the description of the prior art, the invention is directed to a method of processing the collagen tissue of animal or human origin and correspondingly treated biological tissue, and the biocompatibility and durability of the fabric increases so that the use of tissue in the body can radically increase in the optimal case, up to the constant use.
The basic idea of the invention consists in the physical plasma processing biological tissue to solve these problems.
Studies have shown that physical plasma processing in which the gases are exposed to the excitement and success of the radicals, and which chemically neutralizes the toxicity obtained free aldehyde groups due to linking with glutaraldehyde, it is possible, therefore, to achieve significantly improved detoxification compared with the previous level as the first stage to improve implant properties of the tissue. The studies gave statedemocracy over 80%. Thus, closed endothelial surface may be subjected to colonization on surfaces in contact with the blood of the examined tissue.
Chemical process, mainly occurring during the plasma treatment can be briefly described using the example of the application during the process of gaseous oxygen. Thus, the components present in the plasma, namely, oxygen ions and excited oxygen to form carbon dioxide and water in the reaction with the hydrocarbons at the tissue surface. This reaction can thus be used to remove the aldehyde groups present on tissue surfaces, such aldehyde groups, which are formed when linking with glutaraldehyde, resulting in their detoxification.
Preferred conditions for the implementation of gas-plasma method described in paragraphs 2 and 3 of the claims, where it is preferable to use oxygen as the excited gas. However, it can also be applied nitrogen, hydrogen and argon. Energy supply upon receipt of the plasma is preferably carried out using high frequency electromagnetic fields, in particular, the microwave field.
The above gas-plasma techniques are usually carried out with the introduction of a gas that is ionized, rolled back, i.e. sparse processing chamber. Also, who's the one to carry out plasma treatment, atmospheric plasma, using the plasma jet with the excited reactive gas at atmospheric conditions. The plasma jet is directed onto the surface of the implant, thereby local treatment. It happens so that oxygen ions present in the reactive gas reacts with the hydrocarbons tissue surface and form carbon dioxide and water.
In contrast, when plasma processing at atmospheric conditions there is no need for pre-conditioning the drying fabric and therefore can directly handle even a damp cloth in its original condition, when processing biological tissue above-mentioned plasma gas by the method of in-camera processing to ensure detoxification is very important that before plasma treatment is usually water-containing biological tissue exposed to drying, in particular the effects of vacuum and temperature.
Therefore, the biological tissue during processing survive in the complete absence of water.
Subjected to plasma processing tissue for use in implantation moistened by placing it, for example, in the liquid. So it regains its original consistency and proper execution, for example, becomes permanently flexible and durable for a long time, n is the sample, heart valve of a pig.
Particularly preferred variant of the method according to the invention provided in paragraphs 8-10, according to which the fabric is subjected to plasma processing, is supplied biocompatible metal-containing coating. This coverage is an optional biocompatible component on the surfaces of the implants, which for a long time promotes cell growth.
The preferred method of applying a metal-containing coating is the method of PACVD (plasma-assisted chemical vapor deposition - chemical vapour deposition or gases in a plasma environment, that the coating of the surfaces of plastic materials in artificial medical implants have achieved convincing results in terms of biocompatibility thus treated surfaces. This metallsoderjasimi coating selected from the group of metals consisting of Ti, Ta, Nb, Zr, Hf, Ir, Au, Pd, Pt, Ag and Cu. In this regard, it was proved that titanium is particularly good, and for a long time it was used as a particularly biocompatible material in many kinds of applications of implants. Also mentioned coating materials - silver and copper - can additionally or exclusively be introduced into the coating as antibacterial agents.
Paragraphs 11-14 of the claims relate to biological the first tissues of the animal or human origin, which can be used as an implant in the human or animal. According to this invention, at least the surface which can come into contact with the body, is subjected to plasma processing in accordance with the invention for its detoxification. As described above, the improved biocompatibility by using biocompatible metal-containing coating on biological tissue. Preferably, when the biological tissue is a heart valve prosthesis of the vessel in contact with the blood of the surface mechanical or biomechanical injection pumps blood, closed box for cranial hole or replacing cartilage, bone, tendons, diaphragm, chest wall, the wall of the abdominal cavity or the eardrum.
The invention is described in more detail in the following embodiment.
As an example, the collagen tissue of animal origin used heart valve of a pig, which should be used as implants in the human body.
He is preparing, is released from cells and is fixed, i.e. linked with glutaraldehyde after removal from the animal donor to stabilize the usual way. In this case, the valve is placed in a solution of glutaraldehyde concentration from 0.1 to 0.4% and strengthened in the flowing solution at the bottom is their pressures from 3 to 6 mm Hg within 24-48 hours.
Thus acquired heart valve pigs then slowly dried in vacuum and under the influence of temperature and thus fully dehydrated.
Then perform plasma processing of a heart valve of a pig in the processing chamber. For this purpose, the processing chamber is fully pumped (vacuumized) and then there is introduced oxygen. The plasma is heated through supply of high frequency electromagnetic fields, such as 40 kHz or 13.56 MHz, or upon excitation with microwaves. Gaseous oxygen is present in the processing chamber, is excited and converted into radicals under the related power supply.
This plasma gas acts on hydrocarbon group, CxHyon the surface fortified glutaraldehyde implant in accordance with the following reaction equation:
CxHy+(x+y/4)O2→ x CO2+y/2 H2O
As you can see, the hydrocarbons on the surface of the implant, such as, for example, acetaldehyde, turn into relatively harmless chemical compounds, carbon dioxide and water, which can easily be removed from the surface of the implant.
The above plasma processing next continues to be used for the deposition of metal-containing coating on the surface of the implant. For this purpose, the gaseous precursor is fed into the chamber for coating and split under the influence of plasma energy in its atomic components. Thus obtained ions are deposited on the surface. Usually as a metal-containing coating using PACVD method is mainly applied to titanium. Furthermore, the method itself is described in detail in EP 0897997 B1, using as example the coating on the substrate of a plastic material.
The pressure in the reactor for pretreatment and the coating is from 0.1 to 1030 mbar. When applying plasma ideally the pressure was more than 50 mbar. For pre-treatment working gas (e.g. oxygen) is introduced into the reactor at a volumetric rate of gas 0,04 N/min After stabilization of the final pressure to approximately 1 mbar, capacitive plasma input occurs at a power of 20 watts for 60 seconds. Then the gas supply is stopped and the reaction chamber is completely pumped out. For subsequent coating over the previous Ti[N(CH3)2]4directed carrier gas (hydrogen) in the bulk gas velocity of 0.09 N/min and introduced into the chamber for coating. The duration of the coating is approximately 300 seconds when applied plasma power of 20 watts. Further, the gas supply stops again, and the camera for applying the coating is ventilated.
After this vacuum plasma saturation, and applying cover the Oia heart valves of pigs, them again placed in the liquid, so that they, due to the fluid flow, again acquired its original consistency.
1. The method of processing biological tissue of animal or human origin, such as swine, bovine pericardium or heart valves of the human body, to increase the biocompatibility, including physical plasma processing tissue using a plasma gas method, in which the ionized gas is used, nitrogen, hydrogen, argon or, preferably, oxygen, characterized in that
before the plasma processing gas method the fabric is dried,
- the fabric is subjected to plasma processing, are biocompatible metallsoderjasimi floor,
after plasma treatment the dried implant is subjected to the action of moisture.
2. The method according to claim 1, in which the energy required for plasma is carried out using high frequency electromagnetic fields, in particular microwave field.
3. The method according to claim 1, wherein the plasma treatment is carried out with atmospheric plasma using the plasma jet at atmospheric conditions.
4. The method according to claim 1, wherein the drying is performed under vacuum and temperature.
5. The method according to claim 1, in which metallsoderjasimi coating is applied using a method of chemical deposition of vapors or gases in a plasma the environment.
6. The method according to any one of claims 1 to 5, in which metallsoderjasimi coating selected from the group consisting of Ti, TA, Nb, Zr, Hf, Ir, Au, Pd, Pt, Ag and Cu.
7. Biological tissue of animal or human origin for use as an implant in the human or animal obtained by the method according to any one of claims 1 to 6, in which at least the surface, which can be in contact with the body, after drying, the fabric is subjected to plasma treatment for detoxification, the surface subjected to plasma processing, is covered with a biocompatible metal-containing coating, and the fabric is exposed to moisture.
8. Biological tissue according to claim 7, in which metallsoderjasimi coating selected from the group consisting of Ti, TA, Nb, Zr, Hf, Ir, Au, Pd, Pt, Ag and Cu.
9. Biological tissue according to any one of claims 7 or 8, having a configuration in the form of a heart valve prosthesis of the vessel in contact with the blood of the surface mechanical or biomechanical injection pumps blood, closed insertion cranial hole or replacing cartilage, bone, tendons, diaphragm, chest wall, the wall of the abdominal cavity or the eardrum.
SUBSTANCE: invention concerns medicine, particularly neurosurgery. What is disclosed is a biocompatible, biostatic, non-toxic, translucent, however imaginable, elastic, strong, reliably fastened duraplasty graft made of a spatially cross-linked polymer prepared by photopolymerisation of methacrylic oligomers. A graft represents an elastic translucent double-layer film with one layers being relieved, while the other one is smooth. The relief is blue-coloured. Such colour provides intraoperative imaging of the graft for a surgeon. At the same time, the unit dimensions of the relief drawing are such that the graft is translucent enough to control the hemostasis efficiency. The layer adjoining the brain is smooth. A roughness degree of the smooth layer ranges within 10 to 20 nanometers to eliminate the adjoining tissue ingrowth into the graft and its opacity on service.
EFFECT: improved graft performance.
7 cl, 3 dwg, 3 ex
SUBSTANCE: present invention refers to medicine, more specifically to use of a polymer containing thiol groups, for producing a tissue extension implant where a base polymer is polysaccharide.
EFFECT: polymers containing thiol groups have an antioxidant effect, and are characterised by longed stay on an application site.
13 cl, 4 dwg, 7 ex
FIELD: medicine, pharmaceutics.
SUBSTANCE: invention relates to application in biomedicine, cell technologies, substituting and restorative surgery, in particular for manufacturing matrix-carrier, implanted together with cells into human organism in order to restore functioning of affected organ. Method lies in preparation of solution of polyoxybutirate-polyoxyvalerate (POB/POV) in dichloroethane, after which into solution introduced is icy acetic acid with the following component ratio, g: POB/POV - 1-2, dichloroethane- 10-12, icy acetic acid - 10-15; obtained solution is frozen in form at temperature minus (25-30)°C and sublimation drying of obtained semi-product of matrix is performed in vacuum chamber. Freezing of solution and sublimation drying of obtained semi-product of matrix are performed on padding, made from polytetrafluorethylene fiber and plate from water ice. Thickness of water ice plate is selected on condition of its complete sublimation during drying in vacuum chamber, sublimation drying of matrix semi-product on padding being performed on mesh from plastic threads. Initial pressure in vacuum chamber must be not lower than 100 Torr, and after dichloroethane evaporation from matrix semi-product pressure in vacuum chamber is reduced to ~0.01 Torr. Polymer matrix with bimodal structure of simply connected pores is produced.
EFFECT: simplification and cheapening of technology due to refuse from low temperature of performing vacuum sublimation drying.
7 cl, 4 dwg
SUBSTANCE: articles based on biologically active polymer materials can be used in surgery when treating wounds and as materials for temporary replacement of body tissue, in biotechnology for obtaining matrices for growing cell cultures, in pharmaceuticals as carriers of enzymes and other biologically active compounds. The cross-linking reagents are 2,4-derivatives of 3-oxa glutaric dialdehyde (2,2'-oxydiacetaldehyde). These compounds can be obtained via periodate oxidation of monosaccharides, nucleosides and nucleotides.
EFFECT: low toxicity and biocompatibility of chitosan-based materials is achieved by using cross-linking reagents.
2 dwg, 12 ex
SUBSTANCE: composite contains a reactive ceramic phase of triple-substituted calcium phosphate and an organic phase containing polyvinyl alcohol hydrogel. The mechanical properties and injectability of said material can be adjusted by varying the concentration of two phases.
EFFECT: preparation of the new injectable composite applicable as a bone filler.
9 cl, 2 tbl
SUBSTANCE: connecting element (10) is described, in particular a suture material for surgical application, which contains the first material (12) which is substantially rigid when its opposite sides are affected with respectively short-term tensile load, and the second material (11) connected with first material. The second material is substantially rigid when its opposite sides are affected with tensile load, and is made with the ability for slow shortening during the second period of time, longer than the first period of time.
EFFECT: connecting element shrinks over long periods of time and is rigid at short-term fast-increasing loads.
23 cl, 22 dwg
SUBSTANCE: there are described new materials and methods for preparing a titanium dioxide coatings for osteointegrated biomedical prostheses. The invention also refers to an endosseous implant containing biologically compatible metal materials; characterised by the fact that specified implant has a coating containing a nanocrystalline material, containing nanoparticles of formula (I) AOx-(L-Men+)j; (I) where AOx represents TiO2 or ZrO2; Men+ represents metal ion exhibiting antibacterial activity, with n=1 or 2; L represents a bifunctional organic molecule which can simultaneously contact metal oxide and with metal ion Men+; and i represents a number of L-Men+ groups fixed to one AOx nanoparticle.
EFFECT: coatings are formed by nanomaterials exhibiting antibacterial properties and provide osteointegration of implants and, at the same time, reduce rejection peculiar to inflammatory processes caused by infections which can develop next to implants.
19 cl, 1 dwg, 4 tbl
SUBSTANCE: invention refers to the field of polymer chemistry and medicine, namely to method for obtaining thromboresistant polymer materials which have widespread application in medical industry for manufacturing workpieces on blood contact, for example blood-vessels prostheses, parts of bioartificial organs implanted into living body, bloodlines for artificial blood-circulation apparatus, storages for storage and blood transfusion etc. Method for obtaining thromboresistant polymer materials implies mixture polymer with extender, and is inert to blood coagulation water-soluble compounds in amounts of 0.3-3.5 wt % used as extender.
EFFECT: invention enables to produce thromboresistant polymer materials with lowered tendency to adhesion of platelets and lowered ability to formation of fibrinous thrombs on the surface of material in the absence of influence of whole blood coagulation system, as evidenced by increase of buildup time of fibrinous clod from 60-80 seconds to 110-240 seconds.
3 tbl, 36 ex
SUBSTANCE: invention relates to field of medicine, namely to cardio-vascular surgery, and can be used in manufacturing bioprostheses intended for heart valve prosthetics. Essence of invention lies in the following: after conservation with epoxy compound, such as 2-5% solution of ethylene glycol diglycidyl ether, during 2-21 days biomaterial is washed in 0.9% sodium chloride solution and treated with 0.05-1.0% solution of 3-amino-1-oxypropylidene-1,1-diphosphonic acid (DP), prepared on phosphate buffer pH 4.0-8.0. Treatment is carried out at temperature 5-40°C. After treatment biomaterial is washed from non-bound DP and placed into sterilising solution for storage. Application of DP as anticalcium agent gives epoxy-treated biomaterial additional resistance to calcification.
EFFECT: invention ensures increase of treatment efficiency due to reduction of calcium-binding activity of biological prostheses for cardio-vascular surgery.
2 cl, 1 tbl, 1 ex
SUBSTANCE: invention claims biosynthetic implant including semipermeable barrier for maintenance or prevention of diffusion of predefined substances/materials/cells/cell lines formed in human organism from one to the other barrier side, and for maintenance or prevention of diffusion of the same or different predefined substances/materials/cells/cell lines from the other side to the first side. Semipermeable barrier has bioactive surface coating at least one side, e.g. titanium coating, enabling indicated diffusion. Method of connective tissue forming/growth risk reduction for implantation involves implant with semipermeable barrier featuring permeable bioactive material coating at least one side. For example, implant can be applied in biosynthetic pancreas.
EFFECT: biosynthetic implant with reduced connective tissue forming/growth risk after implantation.
16 cl, 10 dwg, 8 ex
SUBSTANCE: group of inventions refers to medicine, and can be used for production of transplantable tissues. A method for cell-free organic tissue preparation for living cell reimplantation involves cell-free tissue preparation on a substantially flat surface and creation of a number of holes on the surface, spaced along the whole surface mentioned above and arranged so that they penetrate deep down at least a portion of thickness of said tissue with the holes designed for keeping the reimplanted living cells. Said number of the holes is created by means of one or more metal needles connected to a power supply which on each needle point induces current flow of such intensity and of such waveform to provide adequate energy for destruction of molecular couplings comprising organic tissue near to the needle point mentioned above. Each hole is formed by current flow and is great enough that the needle point mentioned above could penetrate into a space formed by opening of the molecular couplings. The group of inventions also refers to cell-free organic tissue for living cell reimplantation produced by said method.
EFFECT: group of inventions enables reducing time of producing transplantation tissue and provides living cell penetration along the whole thickness of cell-free tissue.
24 cl, 4 dwg
SUBSTANCE: acellular transplant is described comprising (i) a connected skeletal matrix with openness of pores of biologically and pharmaceutically usable material and (ii) human serum. In accordance with a particularly preferred embodiment, the matrix also contains a gel. Also the method of manufacturing such acellular transplant is described when the matrix and the gel contact with human blood serum. If necessary, the transplant and the serum can be dried. Alternatively, the matrix and the gel can be presented in a dry form prior to contacting. Use of acellular transplant for regeneration of tissues and in particular for regeneration of cartilage and/or bones is also described.
EFFECT: transplant is easy to manufacture, long stored and easy to use.
16 cl, 2 dwg, 2 ex
SUBSTANCE: there is offered method for chemical treatment of xenopericardium that involves chemical stabilisation of xenopericardium with 0.625% glutardialdehyde and following processing with 1% sodium dodecyl sulphate; chemically stabilised xenopericardium is additionally processed with 0.05÷0.25% aqueous solution of chitosan or metal-containing chitosan with deacetylation degree 50÷98% and molecular weight 4÷140·103 at pH 3÷5; upon termination of processing, xenopericardium is fixed in 70% aqueous solution of ethanol; then modified xenopericardium is kept in 0.10÷0.50% aqueous solution of chitosan N-sulphosuccinate with molecular weight 10÷166·103 or chitosan 3,6-O-disulphate with molecular weight 7÷180·103 at pH 4÷8 during 20÷60 mines at temperature 20÷30°C with following fixation in absolute ethanol.
EFFECT: improved durability and biocompatibility of bioprostheses.
3 ex, 7 tbl, 3 dwg
SUBSTANCE: method involves chemical stabilisation of biotissue with 0.625 % aqueous solution of glutaraldehyde, pH 7.4, followed with preparation with a surface-active substance and quadruple change of a working solution. Immediately ahead of implantation, bioprostheses are thoroughly washed with sterile physiologic saline sixfold changed, at 500 ml of the solution for 100 g of biotissue. Then it is processed with 0.05-0.5% aqueous solution of chitosan N-sulphosuccinate of molecular weight 50-150 kDa in intensive stirring during 0.5-2 h with pH within 5 to 8, and to temperature 22±2°C. Further, it is fixed in sterile absolute ethanol and put in sterile physiologic saline, and stored at temperature 6-8°C before implantation.
EFFECT: improved durability of bioprostheses.
5 ex, 4 tbl
SUBSTANCE: bone of natural origin is cleaned, sawed up to 0.2-2.0 cm thick plates, washed with heated to 65°C 0.1 M pH 5.8-6.0 phosphate buffer, digested in 0.1-0.4% activated papain solution at 65°C during 24 hours, then washed in five volumes of water at 40-80°C, treated with 0.4 N alkali at room temperature during 10-24 hours, rinsed in running water, degreased in ethanol/chloroform mixtures in ratio 1:2 firstly, and 2:1 secondly, decalcified in 0.4-1 N hydrochloric acid, treated with 1.5-3% hydrogen peroxide during 4 hours, washed with purified water, then with ethanol, dried at room temperature, packed up and sterilised. Material for osteoplasty and tissue engineering represents compound, in which native collagen matrix space structure and natural bone mineral component are preserved, containing 25% collagen and 75% mineral matter. According to dry material analysis it includes less than 1% non-collagen proteins.
EFFECT: method improvement.
3 cl, 5 ex
SUBSTANCE: autograft is made as mixture of minced muscular auto-issue with the concentrated serum autofibronectin in proportion 1:(0.2-0.5).
EFFECT: autograft reduces the treatment and prevents the post-operational complications.
SUBSTANCE: bone-and-mineral product contains porous bone mineral particles produced from natural bone and having crystalline structure practically corresponding to natural bone structure and practically containing no endogenous organic material. The particles have fibers of physiologically compatible type II resorbable collagen at least on their surface. Mass proportion of type II collagen fibers and porous bone mineral is at least equal to approximately 1:40.
EFFECT: enhanced effectiveness in recovering combined injuries of cartilage and bone tissue in articulations having defects.
8 cl, 6 dwg
SUBSTANCE: present invention relates to resorbable block copolymers for producing resorbable surgical or therapeutic implants for humans and animals. The block copolymer is of type AB or ABA. Block A is formed by polyester and block B is formed by polyether. Type AB has the formula: E-(O-D-CO-)n-(O-CH2-CH2-)m-O-F, where the structural link E-(O-D-CO-)n forms block A, and link -(O-CH2-CH2-)m forms block B. Type ABA has the formula: E-(O-D-CO-)n-(O-CH2-CHr)m-O-(CO-D-O-)n'-E, in which links E-(O-D-CO-)n and E-(O-D-CO-)n. form block A, and link -(O-CH2-CH2-)m forms block B. Content of block B ranges from 0.1 to 4 wt %. The invention also relates to a method of producing and cleaning said block copolymers contaminated with monomers.
EFFECT: obtaining block copolymers or implants from said block copolymers, characterised by high mechanical strength, elasticity, viscosity and fast kinetic resorption.
42 cl, 3 tbl, 4 ex
SUBSTANCE: group of inventions refers to sterilisation and may be used to check sterilisation parameters in a post-arc chamber. The sterilisation indicator comprises a compound heated when contacting oxygen and/or nitrogen atoms, and a thermochromic pigment thermally contacting said compound. Besides, the indicator may consist of two portions one of which contains the compound heated when contacting oxygen and/or nitrogen atoms, and the thermochromic pigment thermally contacting it, and another one is the thermochromic pigment free from the compound heated when contacting oxygen and/or nitrogen atoms. The group of inventions refers to using the indicator in a sterilisation device for oxygen and/or nitrogen atom indication and temperature indication in the post-arc chamber. Also, the group of inventions characterises versions of the method for oxygen and/or nitrogen atom indication in plasma in the post-arc chamber and temperature indication in the post-arc chamber by means of declared sterilisation indicator contacting plasma and comparison of the indicator pigment colour with reference colours.
EFFECT: group of inventions enables checking the sterilisation parameters for object sterilisation with using plasma on the basis of nitrogen or oxygen.
16 cl, 3 dwg, 2 ex
SUBSTANCE: invention refers to disinfection, and can be used in various industries. Materials are treated by low-temperature nonequilibrium plasma of low-voltage capacitive electric discharge at electric field intensity within 5×10 4 to 3×10 6 V/m, frequency within 0.05 to 100 kHz, gas pressure 0.4 to 125 kPa, and magnetic field intensity maintenance within 500 to 1000 E.
EFFECT: invention proves high effective antiseptic material preparation with enabled preparation of fluid and semisolid mediums.
1 tbl, 3 ex