Diagnostic technique for severe clinical course in gastric ulcer
SUBSTANCE: with using indirect immunofluorescence and monoclonal antibodies, the patients suffering gastric ulcer (GU) are examined for an expression level of CD8, CDDR markers by peripheral blood lymphocytes with the CD8 level exceeding 24.5% and the CDDR level exceeding 15% enabling diagnosing a severe clinical course of GU.
EFFECT: use of the offered diagnostic technique provides high information value in diagnosing the severe clinical course of gastric ulcer, well-timed prediction of the severe clinical course of GU, instant diagnosing, provides well-timed and adequate complex of therapeutic measures.
1 tbl, 1 ex
The invention relates to medicine, in particular to therapy, gastroenterology, immunology. It can be used in the clinic for the diagnosis of severe peptic ulcer disease (PUD) in adults.
A known method for the diagnosis of severity YAB in the number of exacerbations of the disease during the year. The patients with three or more exacerbations per year can be considered a disease with a severe course. The disadvantages of this method are: the subjective evaluation of the test feeling sick, which can lead to errors in the diagnosis of the severity of the disease and choosing the wrong treatment. In addition, for the first time showed worsening YAB is not possible to estimate the future severity of disease, abdominal pain and dyspepsia associated with the error power can be perceived by patients as the aggravation, and, on the contrary, weak expression of complaints during the next exacerbation may not attract the attention of the patient. (Order of the Ministry of health of the USSR "on the procedure for conducting a General medical examination of the population" №770 from 30.05.1986 - P.62, (in edition of Order of Ministry of health of the Russian Federation No. 270 from 12.09.1997).
There is a way to diagnose the severity YAB according to the degree of contamination of the coolant Helicobacter pylori. The disadvantage is that the degree of contamination is estimated by the results of several biopsies of the coolant, but the degree of contamination can be servname is Noah, in addition, the invasive method is traumatic. (Arwin LI, Kapeller L.L., Isakov V.A. Morphological diagnosis of diseases of the stomach and intestines. - M.: Triada-X, 1998 - S. 483).
There is a method of determining the severity of the disease state fractions of water in the composition of biomacromolecule formed elements of blood. The heavier for patients, the observed greater loss of associated and increased free fraction of water. The disadvantages of this method is the ambiguity of interpretation of the results, the use of complex, non-traditional methods. (Patent for invention №2312584, 2007, C.1).
YAB is characterized by different etiology, complex pathogenesis of chronic relapsing course, morphological equivalent in the form of a defect in mucosal and submucosal layers with outcome in a connective tissue scar. In patients with severe during periods of remission short, not more than 3-4 months in a year, pain and dyspeptic syndromes are much. This disease is a cause of prolonged suffering of a large number of people, significantly impairing their quality of life. In addition, the disease is accompanied by a high level of destructive and neoplastic complications leading to trucapture, disability, death, which entails an increase in Econ the economic losses for society as a whole.
Due to the large number of syndromic clinical manifestations YAB for diagnosis necessary clinical and biochemical blood tests, fecal occult blood, histological and cytological examination of biopsy specimens, urease test, esophagogastroduodenoscopy with biopsy and brush Cytology, ultrasound examination of abdominal cavity organs and other methods. Additional studies are under suspicion of a malignant ulcer, the presence of complications and comorbidities.
These methods are combined with the data obtained in the study of the clinical picture and history, allow more reliability to establish the existence of YAB and the severity of its course. (Arwin LI, Kapeller L.L., Isakov V.A. Morphological diagnosis of diseases of the stomach and intestines. - M.: Triada-X, 1998 - 483 C. the Order of the Ministry of health of the USSR "on the procedure for conducting a General medical examination of the population" №770 from 30.05.1986 - P.62 (in edition of Order of Ministry of health of the Russian Federation No. 270 from 12.09.1997). Patent for invention No. 2312584, 2007, C.1). The disadvantages of the methods used diagnostic YAB is the long duration of time associated with the use of a large number of instrumental and special methods of research, the ambiguity of interpretation of the results. In addition to the CSO, most studies are of high cost, the involvement of consulting physicians of other specialties, the absence, in most cases, medical documentation that contains a full history.
The aim of the invention is to increase the efficiency differential diagnosis of severe YAB by determining the level of expression of the markers CD8, CDDR the peripheral blood lymphocytes, which leads to faster diagnosis, timely forecasting the severity of YAB, ensuring greater efficiency and more rational selection of therapeutic interventions.
The essence of the proposed method of diagnosis is that patients YAB determine the degree of expression of cell markers CD8, CDDR method of indirect immunofluorescence using monoclonal antibodies and the level of CD8 more than 24.5% and CDDR more than 15% of diagnosed severe YAB.
Determined the number of CD8 and CDDR markers. CD8 is a marker killer-cytotoxic lymphocyte subpopulations, characterizing the activity of the inflammatory process on the cell type, which occurs when YAB. CDDR-marker of the second stage of lymphocyte activation, describing the severity of their activation, which is also the case for YAB.
The dynamics of the immunological changes observed in patients with severe YAB p the relation to light and moderate flow, characterized by a pronounced immune inflammation cell type (possibly Thl response) involving killer-lexically subpopulations of lymphocytes (CD8) and a high degree of their activation (CDDR). In this regard, the determination of the degree of expression of cell markers CD8, CDDR can serve as a diagnostic criterion of severe YAB.
The method is as follows: from the sample of heparinized blood samples (4-5 ml), taken on an empty stomach in patients with PUD emit cells at the gradient ficoll-urografin.
Then 1-0,1 million cells in a volume of 50 μl contribute to the wells of 96-well round-bottom of the tablet. To the cells, add 5 µl of the tested monoclonal antibodies (CD8, CDDR) and incubated for 30-45 min at +4°C. Then add 150 μl of Hanks solution and centrifuged 5 min at 200 g. After removal of the supernatant contribute 50 μl of Hanks solution, the cells are suspended, add 150 μl of Hanks solution and centrifuged 5 min at 200 g.
To the precipitate washed cells add 50 μl of F(ab~)2 fragments of sheep antibody to mouse Ig labeled with FITZ and diluted 1:100 (for cultivation using the Hanks solution containing 0.5% gelatin and 0.1% sodium azide). Cells are suspended and incubated for 30 min at +4°C, the cells are then washed 2 times as described above.
Stained cells analyzed by fluorescence microscope. Cells are suspended, transferred to a subject article is Clough, cover cover glass and looking at drug under immersion with magnification (×3; lens ×90).
The number antigenpositive cells is defined as the percent of fluorescent cells while browsing 200 lymphocytes minus % fluorescent cells observed in the preparation of the negative control. As a negative control, using drugs, prepared similarly, but without the processing of monoclonal antibodies. On the expression of cell markers CD8, CDDR judged severe YAB.
Patient A., 46 years old, taxi driver. Went to the clinic with complaints of mild pain in the epigastric region, occurring in 1.5-2 hours after a meal, and a night of pain, ending after eating, heartburn, nausea. These complaints concerned within 6 days. Medical help is not sought, took but, silos and soda, which gave a temporary effect. Smokes 20 cigarettes a day.
Physical examination: General condition of moderate severity. The body temperature of 36.6°C. the Skin skin color. The vesicular breathing, 18 in 1 min heart Rhythm correct 68 minutes HELL 110/70 mm Hg Tongue coated white bloom. The abdomen is soft, painful epigastric and the area of the projection of the pylorus (2 cm to the right of the navel). Liver, spleen not enlarged. Symptom of Pasternack tricate the capacity. Physiological functions are normal.
History: 3 years ago for the first time diagnosed with peptic ulcer of the duodenum. Treatment at the time of inspection: omez 20 mg 2 R/d 10 days, Almagel 1 tablespoon an hour after meal 5 days. More care was not addressed. 3-4 times per year notes above symptoms. Took omez, ranitidine.
These additional methods.
Complete blood count: HB 140 g/l, er 4,1*1012/l L to 7.2*109/l, erythrocyte sedimentation rate of 7 mm/h.
Urinalysis: specific gravity 1020, the color is straw yellow, the reaction of the acid, L 1-2 p/SP er 0-1 in the p/SP.
ECG: sinus rhythm, heart rate of 72 per minute, the normal position of the EOS.
CCF: lung fields clear, heart without visible pathology.
Ultrasound of abdominal organs: no visible pathology.
The fecal occult blood test (reaction of yens Gregersen collection): negative. Fibrogastroduodenoscopy: ulcer bulb duodenal ulcer twelve 0,4*0,6 cm, chronic erosive gastritis, Bullit. Cicatricial deformity of the duodenal bulb 2-3 degrees.
Urease test: Helicobacter pylori dramatically positive
Microscopy of smears prints gram stained biopsy specimens from the antrum and body of stomach: Helicobacter pylori typical spiral shape in a very large number.
The level of expression of markers CD8 28,60%, CDDR 18.4% of lymphocytes per fericelli blood. That is, CD8 was more than 24.5% and CDDR more than 15%. That allowed us to diagnose Peptic localization of ulcers in the bulb 12 SC intestine, severe degree, 2 degree, acute stage, and assign timely treatment.
Concomitant chronic erosive gastritis.
Complication: cicatricial deformity of the duodenal bulb 2-3 degrees.
A method for the diagnosis of peptic ulcer disease in adults in the level of expression of markers CD8, CDDR by peripheral blood lymphocytes was tested in the comparison groups between 38 patients of mild severity YAB, 45 patients with an average severity of YAB and 10 patients with severe YAB.
All of the respondents had performed blood sampling and determination of the level of expression of markers CD8, CDDR the peripheral blood lymphocytes of the above method.
The results of the study are presented in the table.
The data obtained showed that under severe flow YAB level markers CD8, CDDR significantly increased compared with moderate and mild degree of severity YAB. Diagnosis in the studied groups was confirmed by the main diagnostic methods.
For processing the received data, and statistical analysis in the studied groups was used for the statistical processing of information "STATGRAPHICS PLUS". Vice the Lyali average and the average error (M±M). Comparison of study groups conducted in pairs, using the parametric methods because the study sample could be approximated by a normal distribution. The comparison was performed using t-test (t) (comparison means) and Fisher (comparison of mean square deviations). The confidence level was assessed value "p". Differences in the comparison was considered significant with a probability of more than 95% at P<0,05.
Thus, the obtained data showed that in severe YAB level markers CD8, CDDR increases significantly compared to the average and lightweight for YAB, therefore, the level of expression of CD8 molecules, CDDR the peripheral blood lymphocytes can be considered highly sensitive markers of severe flow YAB.
Using the proposed diagnostic method has the following advantages:
1) high information content for the differential diagnosis of severe peptic ulcer disease by determining the level of expression of CD8 molecules, CDDR the peripheral blood lymphocytes;
2) timely forecasting of severe YAB, because this will ensure efficiency and rational realization of a complex of therapeutic measures for patients with YAB;
3) rapid diagnosis, since it is only necessary to determine the level of expression of markers CD8 CDDR the peripheral blood lymphocytes;
4) due to rapid diagnosis ensures timely and efficient implementation of the complex of therapeutic measures.
|CD8 (%) M±M (min, max)||CDDR (%) M±M (min, max)|
|Note: * - p<0,05 - significance of differences between groups when compared with severe YAB.|
|**p<0,05 - significance of differences between groups when compared with easy for YAB.|
Method for the diagnosis of severe peptic ulcer disease, including the study of the peripheral blood, characterized in that the hold determining the extent of expression of cell markers CD8, CDDR method of indirect immunofluorescence using monoclonal antibodies and when the content in the peripheral blood markers CD8 more than 245%, CDDR more than 15% of diagnosed severe peptic ulcer disease.
SUBSTANCE: method involves the peripheral blood analysis for T-lymphocyte count with receptor expression in relation to CD3+ and CD25+ cells by cell count in 1 mcl of blood before the beginning of treatment and 10 days after. If observing the count increased 7% and more in comparison with the reference, a therapeutic effect in non-psychotic versions of psycho-organic syndrome is predicted to be positive.
EFFECT: use of the method enables more precise prediction of the clinical effectiveness in the given disease that promotes selecting an adequate therapy and reducing length of treatment.
SUBSTANCE: invention describes a method of detecting the decreased phosphatidyl ethanolamine content in red-cell membranes in a pregnant woman suffered aggravated herpes-virus infection in their third trimester of pregnancy with underlying increasing peripheral blood TNFα, characterised by the fact that peripheral blood of the pregnant woman is analysed for the TNFα content to detect phosphatidyl ethanolamine in red-cell membranes by two-dimensional thin-layer chromatography, and a discriminant equation is presented. Df=+1.743×TNFα+(-1.079×phosphatidyl ethanolamine), and if Df is more than a boundary value 78.86, the decreased phosphatidyl ethanolamine content in red-cell membranes is detected.
EFFECT: higher red-cell membrane stability in peripheral blood of the pregnant woman.
SUBSTANCE: invention describes a method for prediction of developing suppurative complications of acute pancreatitis involving laboratory blood analysis wherein the patients are examined for the kinetics of leukocyte chemiluminescence with using an activated suspension of clinical strains Acinetobacter baumannii or Pseudomonas aeruginosa or methicillin-resistant Staphylococcus aureus - MRSA and combining maximisation time - T max more than 1400.0 sec., maximum intensity - 1 max less than 5131.5 standard units, light sum - S less than 144000.0 standard units for the reaction initiated by Acinetobacter baumannii, and combining the values T max>1435.0 sec., I max<4708.0 standard units, S<118000.0 standard units for the reaction initiated by Pseudomonas aeruginosa, or combining the values T max>3520.0 sec., I max<4257.0 standard units, S<169000.0 standard units for the reaction initiated by MRSA enables predicting developing suppurative complications.
EFFECT: higher sensitivity and reduced time of prediction of suppurative complications of acute pancreatitis ensured by enabled assessment of the specific properties of patient's phagocytic response to etiologically significant activators.
1 ex, 4 tbl
SUBSTANCE: invention describes a method of assessment of the effect of arginine dehydrogenase activity on the blood histamine level in pregnant women suffered aggravated herpes viral infection with herpes antibody titre 1:12800 in the third trimester of gestation wherein syncytiotrophoblast of placental villi are examined with a histochemical reaction; section regions are photographed, entered in the computer and cytophotometried; observing increasing arginine dehydrogenase activity up to 48.7±2.9 standard units enables stating increasing the peripheral blood histamine level of the pregnant woman up to 1.44±0.09 mcmole/l that exacerbates foetoplacental deficiency in pregnant women.
EFFECT: method allows assessing the effect of arginine dehydrogenase activity on the blood histamine level of the pregnant women suffered aggravated herpes viral infection in the third trimester of gestation.
SUBSTANCE: pre-therapy blood plasma is analysed for immunoglobulin E, adrenocorticotrophin and endorphin, the index SCORAD dynamics is determined by formula: Dsk=1.34147-0.000820741*IgE-0.00382642*ACTP+0.0209401*endorphin wherein IgE is the level of acute blood plasma immunoglobulin E mIU/ml; ACTP is the level of acute blood plasma adrenocorticotrophin ng/l. If observing the Dsk value exceeding 1.18, a prognosis of improving clinical dynamics of recovery is favourable; the conventional therapy is considered to be sufficient. The Dsk value being equal to 1.18 or less, the improving clinical dynamics of recovery is unfavourable, the intensified therapeutic scheme for atopic dermatitis is considered to be preferential.
EFFECT: higher prediction accuracy.
SUBSTANCE: what is presented is a method for prediction of a character of an uterine involvement in myomatous nodes in a female patient suffering hysteromyoma involving DNA recovery from peripheral venous blood, analysis of +36 A/G locus genotypes of type 1 tumour necrosis factor receptor TKFR1 and analysis of myomatous nodes, and also measurement of an uterus. If detecting the genotypes +36 GG and +36 AG TNFR1, a lesser degree of the uterine involvement is predicted. The genotype +36 AA TNFR1 enables to predict a greater degree of the uterine involvement.
EFFECT: using the presented method allows predicting the character of the early uterine involvement in myomatous nodes.
4 dwg, 1 tbl, 1 ex
SUBSTANCE: for the purpose of diagnosing metabolic compensation disturbances and predicting a risk of developing complications in the patients suffering type 2 diabetes mellitus (type 2 DM), peripheral venous blood is examined for a percentage of apoptotic lymphocytes An+/Pl-. The lymphocyte An+/Pl- value below 3.5 % enables diagnosing a condition of carbohydrate metabolism compensation and predicting a favourable clinical course of the disease for the following year. The lymphocyte An/PI- content within the range 3.5 to 6% provides diagnosing a condition of subcompensation and a moderate risk of developing complications. The An+/Pl- value exceeding 6 % described a condition of decompensation and a high risk of developing chronic complications of type 2 diabetes mellitus.
EFFECT: higher accuracy of prediction and reduction of the risk of developing complications in diabetic patients.
SUBSTANCE: invention discloses a method of solid-phase immune-enzyme assay wherein as an antigen, an equimolar mixture of synthetic peptides is immobilised on plastic with the mixture containing: nonapeptide (position 125-133), tridecapeptide (position 208-218) of an amino acid sequence of a human β1 adrenoreceptor molecule and a chimeric construct of the same peptides jointed by a disulphide bond. The presented method shows substantially greater sensitivity in detecting the presence of β1-adrenoreceptor autoimmune antibodies in blood plasma and serum in the patients with dilatation cardiomyopathy.
EFFECT: invention is also applicable for diagnosing the patients with various cardiovascular diseases.
1 dwg, 4 ex
SUBSTANCE: oropharyngeal or nasal biological material is sampled to recover DNA; it is followed by the PCR with using three primers simultaneously: Epstein-Barr gene (EBV) LMP, Cytomegalovirus gene (CMV) MIE, type 6 human herpes virus gene pol (HHV-6). A human 3-globin gene is used as an internal reference sample (IRS). Data derived from the PCR amplification are used to calculate the quantitative content of specific viral DNA in the analysed sample by formula. An index is determined for each viral DNA, while the blood sample is analysed for the quantitative content of a chemical contaminant technogenically significant in the territory; it is followed by stating the presence or the absence of the contaminant exceeding its reference value; total value of EBV, CMV, HHV-6 indexes is calculated, and if its value exceeds 1.0 and the blood chemical contaminant concentration is simultaneously increased, a secondary immunodeficient disease is diagnosed.
EFFECT: invention enables characterising the quantitative content of three viruses of a herpetic group in a human body simultaneously and assessing the combined effect of viral and chemical loads on the immunity.
1 tbl, 3 ex
SUBSTANCE: for the purpose of detecting a level of circulating immune complexes (CICs), a precipitate of human blood serum in polyethylene glycol is prepared, centrifuged and dissolved. Total CIC level is determined either by optical density at wave length 280 nm and 260 nm, or by Lowry method; a complement-binding CICs are detected. The CIC precipitate is prepared with using a buffer containing 10% PEG 3350 in the ratio 1:1; the serum is incubated for 10 min at room temperature. The complement-unbinding CICs are detected as a difference between total CIC level and the complement-binding CIC. The group of inventions also concerns a test system for detecting the CIC level in human blood serum. The test system contains a CIC precipitation buffer representing 10% PEG in 0.01 M Tris-HCl-buffer, pH 7.4, as well as a dissolution buffer for the CIC precipitate representing 0.01 M Tris-HCl-buffer, pH 7.4, containing 0.15 M NaCl and a calibration solution containing human serum albumin in the concentration of 6 g/l in 0.01 M Tris-HCl-buffer, pH 7.4, containing 0.15 M NaCl.
EFFECT: inventions enable higher accuracy of blood serum CIC evaluation, provided in-depth study in immune, autoimmune and oncological diseases, and controlled effectiveness of the therapy.
2 cl, 3 tbl
FIELD: medicine, ophthalmology.
SUBSTANCE: in lacrimal liquid one should detect the content of interleukin 8 (IL-8) and that of interleukin 1 beta (IL-1β) to calculate prognostic coefficient (PC) due to dividing the first value by the second one by the following formula: At PC value being below 10.0 one should predict favorable disease flow, and at PC value being above 10.0 - unfavorable flow.
EFFECT: higher accuracy of prediction.
FIELD: medicine, medicinal microbiology.
SUBSTANCE: method involves growing microorganism culture to be studied in solid nutrient medium followed by preparing microbial suspension and its incubation in the presence of lactoferrin. Control sample is prepared in parallel series. Control and experimental samples are incubated, supernatant is removed from bacterial cells and lactoferrin concentration is determined in supernatant of experimental and control sample by immunoenzyme analysis. Then anti-lactoferrin activity is calculated by difference of concentrations of residual lactoferrin in experimental and control samples. This method provides enhancing the sensitivity and precision in carrying out the quantitative evaluation of anti-lactoferrin activity in broad spectrum of microorganisms that is urgent in diagnosis and prognosis of diseases with bacterial etiology. Invention can be used in determination of persistent indices of microorganisms for assay of their etiological significance in pathological processes.
EFFECT: improved assay method.
3 tbl, 3 ex
FIELD: medicine, biology.
SUBSTANCE: invention relates to nutrient medium used for accumulation of cells for the following cytological and/or immunocytochemical analysis carrying out. Invention relates to medium containing salts NaCl, KCl, anhydrous CaCl2, MgSO4 x 6 H2O, MgCl2 x 6 H2O, Na2HPO4 x 2 H2O, KHPO4, NaHCO3, and also glucose and Henx's solution, 10% albumin solution and polyglucin taken in the ratio 1:1:1. Invention provides enhancing the preservation of cells.
EFFECT: improved an valuable properties of nutrient medium.
FIELD: medicine, cardiology.
SUBSTANCE: in peripheral blood one should detect the level of CD95(+) and CD16(+) neutrophilic granulocytes and at combination of increased level of CD95(+) neutrophilic granulocytes by 4 times and more and CD16(+) neutrophilic granulocytes by 0.6 times against the norm with ECG signs of myocardial infarction one should predict lethal result of large-focal myocardial infarction.
EFFECT: higher accuracy of prediction.
FIELD: medicine, parasitology.
SUBSTANCE: one should carry out immunoenzymatic assay to detect diagnostic optic density and that of labeled immune complex in a plot's hole with tested serum measured in conventional units at wave length being 492 nm. One should calculate coefficient of antibodies concentration measured in conventional units by the following formula: CAC = (Odtsh - Odd) x 100, where CAC - coefficient of antibodies concentration, Odtsh - optic density of the hole with tested serum, Odd - diagnostic value of optic density, 100 - coefficient of serumal dilution. By CAC value one should detect the titer of antibodies to Lamblia intestinalis antigens to interpret results of the trial. The method enables to study the dynamics of disease flow.
EFFECT: higher efficiency and accuracy of diagnostics.
1 ex, 1 tbl
SUBSTANCE: the present innovation deals with studying and treating diseases of inflammatory, autoimmune and degenerative genesis. One should perform sampling of heparinized blood followed by its sedimentation to obtain blood plasma with leukocytes and centrifuging to isolate the latter which are washed against erythrocytic and serumal admixtures, and, also, it deals with calculating the number of cells in samples out of leukocytic suspension after incubation (B) for 1.5 h at 37 C in holes of plastic microplotting board, out of leukocytic suspension one should additionally prepare two samples, one should be applied to calculate total number of leukocytes before incubation (A), the second sample undergoes incubation at the same mode at addition of autoserum to calculate the number of cells remained after incubation (C). One should state upon adhesive properties of leukocytes by the index of spontaneous adhesion (D), where D=(A-B)/B.100%, and effect for enhanced cellular adhesion under the impact of autoserum should be detected by the value of K=(B-C)/C.100% at K ≥ 30%, where B - C - the number of cells undergone additional adhesion after addition of autoserum. The present innovation widens functional possibilities of the suggested method due to obtaining additional values depicting adhesive properties of blood leukocytes.
EFFECT: higher accuracy of detection.
FIELD: medicine, immunology.
SUBSTANCE: one should carry out reaction of blast-transformation, detect proliferation of T-lymphocytes activated with antibodies to CD3 in the presence of interleukin-7 (ACT IL-7) and in the presence of interleukin-7 and dexametazone (ACT IL-7 D), calculate the index for dexametazone action as the ratio of ACT IL-7 to ACT IL-7 D, moreover, the value of dexametazone action index being above 1.2 indicates increased production of cytokins that suppress T-lymphocytes in neonatals. The method enables to detect functional defect of immune system that characterizes neonatal period.
EFFECT: higher efficiency of detection.
SUBSTANCE: method involves measuring forced exhalation volume per 1 s (FEV1) in l, full right ventricle evacuation time (RVE) in ms and angiotensin II value (AII) in ng/l. Discriminant relationship is built as D=0.504·RVE+3.038·FEV1 - 2.0·AII. D being less than 83.88, pulmonary hypertension occurrence is predicted within 1 year. D being equal to or greater than 83.88, no pulmonary hypertension is predicted to occur.
EFFECT: enhanced accuracy of prediction.
FIELD: medicine, medicinal immunology.
SUBSTANCE: method involves determination of heterophilic antibodies in human serum blood by the Paul-Bunnel's method relatively the level of circulating immune complexes, complement-activating properties of heterophilic antibodies by incubation of standardized ram erythrocytes with 0.8% serum for 30 ± 5 min and the following measurement of the erythrocytes lysis degree. The measurement of the effector function coefficient of heterophilic antibodies is carried out by the complement system Keff.f.h.a.-c.s. by the formula: Keff.f.h.a.-c.s. = Y/Tg.a. wherein Y means a lysis degree, %; Tg.a. means a reverse titer of heterophilic antibodies to ram erythrocytes. The damage assay is carried out by comparison of the immune status with the relative level of circulating immune complexes in serum. Method provides detection of preclinic from of immunodeficiency and autoimmune diseases that opens the possibility for their prophylaxis at most early stages of development. Invention can be used for assay of damage in the immune status in human serum blood.
EFFECT: improved method for assay.
5 tbl, 1 ex
SUBSTANCE: method involves concurrently examining anti-inflammatory IL-4 level in blood serum and lacrimal fluid. The value being within the limits of 60-70 pg/l in blood serum and 5-15 pg/l in lacrimal fluid, disease prognosis is considered to be unfavorable. The IL-4 concentration being within the limits of 90-100 pg/l in blood serum and 20-30 pg/l in lacrimal fluid, disease prognosis is considered to be favorable.
EFFECT: high accuracy of diagnosis.