Equipment system for continuous detection and measurement of motor activity of bivalve molluscs

FIELD: physics.

SUBSTANCE: equipment system includes measuring devices connected to a computer. In the system, consisting of one or more sensors, an amplifier, an analogue-to-digital converter (ADC) and a computer, each sensor is a tensoresistor sensor mounted on a horizontal base inside a protective housing and having the end of a flexible elastic sensor plate protruding from the housing, said plate being meant for connection with the top valve of the tested mollusc. The output of each sensor is connected to the input of the amplifier consisting of a zero balancing device, an analogue signal amplifier and a signal shift device connected in series. Each sensor is connected to the bridge circuit of the zero balancing device. The output of the amplifier is connected to the input of a 10 bit ADC whose output is connected to the input of an interfacing device which transmits a digital signal to the input of the computer having special software.

EFFECT: high accuracy and reliability of detection as well as simple design.

2 cl, 3 dwg

 

The invention relates to the field of physiology, Hydrobiology, ecology and environmental protection, in particular to the means of environmental monitoring of the aquatic environment by using non-invasive monitoring of functional status invertebrates, and can be used for remote registration of motor activity of bivalves in their environment for automated rapid assessment of the quality of the aquatic environment in real time.

Known sensors of physiological activity of invertebrates with a hard outer cover (RF patents for utility models No. 52190 from 03.11.05, No. 61431 on 25.08.06 year). Sensors include optical radiation source, a fiber-Opticheskie splitter, N buildings with elements of the unit on the body under test bespozvonochnykh, N receiving optical fibers and N detectors of optical radiation. The known method and system of biological monitoring of the aquatic environment on the basis of the registration provisions of the valves of the shell double shell-bearing mollusks by RF patent for the invention №2361207 from 20.06.2008, the System comprises a fiber optic position sensors of the valves of the clam shell, amplifiers, analog-to-digital Converter and a computer. Each sensor includes a source of optical radiation, a receiver of optical radiation, the transmission line signal is an optical fiber, the sensor base, which is made with the possibility of installing it on the half-shell and with a fixed sensor element made in the form of a loop of optical fiber. On the other the half shell has an element of influence on the sensor with the possibility of its mechanical interaction with a loop of optical fiber. These units are difficult to manufacture and the placing on the body, unreliable in use and not applicable to the real natural conditions because of a change of the total light beam; therefore, they can only be used in artificial, permanent and controlled conditions. In addition, the optical fiber and the photoelectric cell will grow into a suspension, microalgae and bacteria even in the aquarium, which also changes the intensity of light.

Known biological warning system developed in the Netherlands and manufactured under the trademark Musselmonitor (originally in Dutch Mosselmonitor: De Zwart D., K.J.M.Kramer & H.A.Jenner (1995), Practical experiences with the biological early warning system 'Mosselmonitor', Environ. Toxicol. Water Qual. 10: 237-247), applied in many countries of the world mainly for the management of freshwater and, sometimes, coastal waters, and water supply Budapest, Hungary, for quality control of chlorinated drinking water by monitoring the degree of revelation of the shells of bivalves. Musselmonitor (myus eliminator) is a box-shaped housing, hosts several shellfish (for example, 8 copies of the Zebra mussel, Dreissena polymorpha)on both leaf of every specimen glued on electromagnetic sensor, strictly towards each other. Technical deficiencies in the system as follows: system mounting (bonding) sensors and wires are bulky and unreliable, heavy for normal operation shellfish that are vulnerable to dynamic action of currents and waves from the speakers, a relatively large sensors and wires; these wires and sensors are easily susceptible to rapid boonratana (filamentous algae, hydroids, juvenile shellfish and other species)that affect the system. After attachment of each sensor calibration for translation from scale voltage in the distance between the leaves (in mm). The offset parts of the sensor relative to each other (magnet relative to the Hall sensor) even at 1 mm significantly distorts registered as 3-4 mm often is a full range of motion of the valves.

The closest is the system operational biological monitoring and indicating on the application for invention No. 2010118938 from 11.05.2010, the System includes measuring devices connected to the computer. As measuring instruments (measuring instruments measuring and recording unit) can be used Hall sensors, plethysmography strain gauges, volconn the optical sensors, a stationary video camera and/or a web camera underwater video observation of the behavior of aquatic organisms. Measuring and recording unit can be placed on a floating or stationary floating, or on the waterworks or water supply stations.

Declare the instrument complex, as is known, contains measuring devices connected to the computer.

Declare complex of instruments aimed at improving used for biological monitoring of measuring instruments.

The technical result is the use of strain gages for registration and measurement of motor activity of bivalves, improving the accuracy and reliability of monitoring the status of the water environment, simplify design, increase autonomy and reducing the cost of systems biological monitoring and bioindication using these complex devices.

The technical result is achieved by the fact that in the complex, consisting of one or more sensors, amplifier, analog-to-digital Converter (ADC) and a computer, each sensor is a strain sensor mounted on a horizontal base, inside the protective housing and protruding from the housing by the end of the flexible elastic plate of the sensor to its connection with the upper sash those who together with shellfish. The output of each sensor is connected to the input of the amplifier, consisting of a series of interconnected devices balancing zero, the amplifier of the analog signal and the shift signal. Each sensor is connected to a bridge circuit devices balancing zero. The amplifier output is connected to the input 10-bit ADC, the output of which is connected to the input device pairing, transmitting the digitized signal to the input of a computer equipped with specialized software.

Run as a sensor system, a strain gauge mounted on a horizontal base, inside the protective housing and protruding from the housing by the end of the flexible elastic plate of the sensor to its connection with the upper sash test mollusk, ensures the reliability and stability of the Desk in any conditions, simplifying the design of the sensor, increasing their autonomy and reducing the cost of systems biological monitoring their use.

In the present invention for the first time for continuous recording and measurement of motor activity of invertebrates applied strain gauges (load cells).

The execution of the amplifier, consisting of a series of interconnected devices balancing zero, amplifier analogue of the first signal and the shift signal, the entrance of which, namely, bridged device zero balancing is connected to the output of each sensor, and the output of the amplifier is connected to the input 10-bit analog-to-digital Converter (ADC), and connecting the output of the ADC with an input device pairing, transmitting the digitized signal to the input of a computer equipped with specialized software that significantly improves accuracy and facilitates data processing, minimizes distortion and interference. Furthermore, the design of the amplifier allows you to connect one, two or more load cell. Instrument unit can run on a rechargeable battery that ensures its independence.

Thus, for the first time for registration and measurement of motor activity of bivalves used a combination of load cell and sensitive linear signal amplifier with the bridge at the entrance, with further digital conversion signal for providing an interface between a strain sensor that is included in the shoulder bridge device balancing zero, with the software of the personal computer. This together significantly improves accuracy and facilitates data processing, simplifies the design, dimensions and accordingly reduces the cost of monitoring systems using data from the app is the moat.

The analysis revealed when searching for sources of information showed that the proposed set of essential features are unknown in the prior art, which confirms the compliance of the decisions of the criterion of "novelty".

Because the claimed set of essential features allows you to get a new technical result other than the one that provides the known methods, it can be argued that the proposed solution meets the criterion of "inventive step".

The invention is illustrated using diagrams are presented: figure 1 - structural diagram of the system; figure 2 - block diagram of the amplifier figure 3 - connect the dash complex with the test animals.

The instrument set contains (see figure 1, 2) one, two, or more, a strain sensor 1, an amplifier 2, an analog-to-digital Converter (ADC) 3, and directly connected to the device 4 with the transmitter 5 and the display device information 6. As evaluator 5 device information display 6 is used, the personal computer 7. Amplifier 2 produces amplification, filtering and adjustment of the analog signal and consists of serially interconnected devices of zero balancing 8, the amplifier of the analog signal 9 and the device shift signal 10. The amplifier 2 is built on t is adicionou diagram of the operational amplifier available (common) chips. High sensitivity and linearity of the amplifier 2 is achieved by using the input of the bridge circuit with manual balancing scratch in the shoulder which is connected to the output of the load cell 1. The output of the balancing zero 7 is connected to the amplifier input analog signal 8, which, in turn, is connected to the input of the shift signal 10 in the positive region, the output of which is connected to the ADC input 3. The ADC 3 quantized analog signal with a sampling frequency of 10 Hz and digitized. The ADC output 3 is connected to the input devices 4, transmitting the digitized signal to the input of the computer 7. As device pairing can be used for a serial interface USB. For connection to the input device zero balancing two or more strain sensors use the contact wire plug connection.

The load cell 1 (see figure 3) is a thin flexible elastic plate 11, along which glued the wire strain gauge (DMS 12. The load cell 1 is placed in a protective casing 13, is made with a cutout for the plate 11, and is mounted on the horizontal base 14 fixed on the vertical post 15. One end of the plate 11 is connected by a flexible (thread) or rigid (rod, for example) coupling 16 with the upper sash test of clam 17. The attachment connection 16 on the plate 11 define the tsya views of clam 17, and may be in one case, on the end plate, the other closer to the middle. Clam 17 can be placed directly in natural conditions or in the aquarium with water supplied from the test horizon. Figure 3 shows a wiring diagram of the load cell 1 with clam 17, the lower sash is glued to the support 18, is placed in the water at a certain depth (mainly from 0.1 to 2 m). Bearing 18 may be rigidly connected to the platform (floating or fixed) or attached to a pier or other shoreline structure that hosts the load cell 1 and the other constituent parts of the complex (not shown). Support 18 may also be rigidly connected to the load cell 1, for example, by installing them on the same bracket.

This instrument can be used in any system of biological monitoring and display.

Load cell, amplifier and ADC and the computer can be placed on the Bank (including the pier, the pier, etc) or on a floating structure (raft, platform, terminal, pontoon or boat) in a waterproof housing. The power system can be supplied from storage batteries or by connecting to shore power.

Instrument unit operates as follows.

The force from moving the test animal 17 (closing of the valves of the clam shell) is transmitted through the CIDP is eenou to the upper sash mollusk flexible or rigid connection 16 on the elastic plate 11 of the load cell, bending which causes a current change in the strain gauge 12. The signal from the strain gauge 12 is supplied to a semiconductor amplifier 2, where it is amplified, filtered and corrected.

Then this balanced analog signal from the load cell 1, amplified to the required amplitude and shifted in the positive voltage is applied to the input 10-bit ADC 3, where it is quantized with a sampling frequency equal to 10 Hz, and digitized. Further information on the serial interface USB is transmitted to the personal computer 7 for processing and visualization of results.

After the registration information is displayed in the form of a graph of hectogramme on the screen of the personal computer 7 and is stored on the hard disk as a file. In addition to the digitized data are written to the file data on the measure of (name, date, time, duration, sample rate quantization, for more information). Data processing begins with a search for characteristic changes on the chart hectogramme and calculate their statistical values. Next is the procedure of discarding false positives, and the position of the found characteristic changes on the chart hectogramme all determined timeframe and amplitude characteristics of the signal.

The data from the computer 7 can be transmitted via the Internet to the operator or the Central information processing.

The advantages of the proposed system: a minimal impact on the body of the mollusk is attached with glue for a few minutes only the tip of a flexible connection (thin filament) or hard links (arm); reliability of the use of flexible or rigid in natural conditions (tested for 5 years); easy snap connection with the clam provides easy replacement of the mollusk in the case of his death; all parts of the complex easy to manufacture, which reduces the cost of the complex; the complex provides improved accuracy and reliability of condition monitoring of the aquatic environment.

The inventive instrument unit has successfully passed the pilot testing in the natural environment.

1. Complex of instruments for continuous recording and measuring behavioral responses of bivalve mollusks, including measuring devices connected to the computer, wherein the complex comprising one or more sensors, amplifier, analog-to-digital Converter (ADC) and a computer, each sensor is a strain sensor mounted on a horizontal base, inside the protective housing and protruding from the housing by the end of the flexible elastic plate of the sensor to its connection with the upper sash test mollusk, the output of each sensor is connected to the input of the amplifier, sostayashego the serially interconnected devices of zero balancing, amplifier analog signal and a shift signal, each detector is connected to a bridge circuit devices balancing zero, the output of the amplifier is connected to the input 10-bit ADC, the output of which is connected to the input device pairing, transmitting the digitized signal to the input of a computer equipped with specialized software.

2. Instrument unit according to claim 1, characterized in that the load cell, amplifier, ADC and the computer placed on shore or on a floating structure, which is used as anchored raft, floating platform, floating buoys, terminal, ship.



 

Same patents:

FIELD: medicine.

SUBSTANCE: isolated during bacteriologic analysis of quality of water for various purposes pathogenic bacteria, additionally potentially pathogenic bacteria with their further identification and normalised indicators of water quality estimation are taken into considereation with further implementation of process of generalisation into a single assessement of microbial risk thresholds, which is performed by calculating first weighted coefficients by formula: An=(P1·V1+P2·V2+…+Pn·Vn). After that, the data obtained are used to calculate integral index by each sanitary and hygienic factor by formula: Estimation of risk by three-level classifier is considered high if values of Rfac are from 0.4 to 1.0 including 0.4 and 1.0, medium if values of Rfac are from 0.2 to 0.4 and low if values of Rfac are from 0 to 0.2, including 0. Estimation of risk by five-level classifier is considered to be very high if values of Rfac are from 0.6 to 1.0, including 0.6 and 1.0, high if values of Rfac are from 0.4 to 0.6, including 0.4, medium if values of Rfac are from 0.2 to 0.4, including 0.2, low or acceptable if values of Rfac are from 0.15 to 0.2, including 0.15 and very low if values of Rfac are from 0 to 0.15. After that the obtained results are used to calculate generalised index of risk of sexually transmitted bacterial intestinal infections CA by formula by three-level classifier it is considered high if values of CA are from 0.4 to 1.0, including 0.4 and 1.0, medium if values of CA are from 0.2 to 0.4, and low if values of CA are from 0 to 0.2, including 0. Estimation of risk by five-level classifier is considered very high if values of CA are from 0.6 to 1.0, high if values of CA are from 0.4 to 0.6, including 0.4, medium if values of CA are from 0.2 to 0.4, including 0.2, low or acceptable if values of CA are from 0.15 to 0.2, including 0.15 and very low if values of CA are from 0 to 0,15.

EFFECT: application of claimed method makes it possible to reduce or prevent morbidity associated with bacterial semination of water.

1 ex, 8 tbl, 1 dwg

FIELD: medicine.

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1 ex, 2 tbl

FIELD: chemistry.

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1 ex, 4 tbl

FIELD: ecology.

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2 cl, 1 ex, 6 tbl

FIELD: medicine.

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2 ex

FIELD: ecology.

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FIELD: chemistry.

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2 tbl, 4 ex

FIELD: chemistry.

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3 cl, 7 dwg

FIELD: processing procedures.

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4 cl, 2 tbl, 2 ex

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3 cl, 1 dwg, 3 tbl, 3 ex

FIELD: environmental monitoring.

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2 tbl

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2 tbl, 1 ex, 1 dwg

FIELD: analytical methods in industrial sanitation.

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EFFECT: reduced determination inaccuracy.

FIELD: environmental protection.

SUBSTANCE: invention concerns estimation of environmental pollution using bioassay methods. In particular, method is accomplished through bioindication of controlled area using, as bioindicators, internals (muscles, kidneys, liver) of wild hoofed animals (elk, dear, wild boar). One determines content of heavy metals in these organs placed within an area, compares thus obtained data with maximum permissible concentrations of heavy metals in foods, and estimates heavy metal pollution level of the area from resulted difference. Existence of long-term pollution of a region is judged of from excess concentration of heavy metals in wild hoofed animal kidneys and existence of single release of mercury and lead from that in muscles and liver.

EFFECT: enabled multiple estimation of considerable areas at reduced effort.

2 cl, 3 tbl

FIELD: analytical methods in environmental protection and toxicology.

SUBSTANCE: subject of invention is drinking, natural, and waste water quality monitoring. Toxicity of aqueous medium is determined from variation in activity of animal brain plasma membrane Mg2+-ATPase activated by chlorine and/or bicarbonate ions. In one embodiment of invention, toxicity of aqueous medium is determined by mixing above plasma membranes with test aqueous medium adjusted to physiologic pH with phosphorus-free buffer followed by addition of solution containing Tris-ATP and magnesium ion source as well as chloride and/or bicarbonate ion(s) source, incubation until inorganic phosphorus is formed, and determination of toxicity from concentration of inorganic phosphorus. Brain plasma membranes used as indicator contain Mg2+-ATPase capable of being activated by chlorine and/or bicarbonate ions.

EFFECT: extended functional possibilities of method and use of reagent, increased sensitivity, and enabled determination of toxicity at lower concentration of various-type toxicants.

20 cl, 6 tbl

FIELD: environmental protection.

SUBSTANCE: invention concerns evaluation of pollution of areas with pesticides involving bioassay techniques. To that end, area under control is subjected to bioindication using wild hoofed animals (mainly elks, deer, wild boars) as bioindicators. Within specified period of time, animal internals are sampled, pesticide content therein is determined, and thus obtained results are compared with maximum permissible pesticide levels for food products. Comparison data are used to estimate quality of media.

EFFECT: increased representativeness of monitoring results, enabled evaluation of a vast region or local area or local agrocenosis at lower effort.

2 cl, 2 tbl

FIELD: chemistry, water quality control, method for quantitative estimation of organic substance properties in aqueous solutions.

SUBSTANCE: indicator plate is immersed in aqueous solution and according to alteration of aqueous solution composition chemical activity of organic substances in this solution is determined. In clamed method tree vessels are used. Two vessels contain aqueous solution to be tested and the third vessel contains control aqueous solution free from organic contamination. In aqueous solutions containing in the second and third vessels indicator plates are immersed, then aqueous solutions in all vessels are heated up to 95-105°C, held at this temperature for 55-65 min, cooled to 15-25°C, filtered though membrane filter with pore size of 0.46 mum, then iron content is measured in all vessels and chemical activity of organic substances in aqueous solutions is calculated according to equation ka=ΔFe/Fe1, wherein ΔFe = Fe2- Fe1- Fe3; Fe1 is iron content in the aqueous solution of the first vessel; Fe2 is iron content in the tested aqueous solution of the second vessel; and Fe3 is iron content in the aqueous solution of the third vessel.

EFFECT: simplified method with enhanced functionality.

3 ex, 4 tbl

FIELD: ecological engineering, particularly river monitoring with taking into consideration river pollution with sewage water within the limits of cities and other inhabited localities.

SUBSTANCE: method involves choosing river observation points relative single pollution source or pollution source array; aligning one observation point with single pollution cross-section or pollution cross-section array; taking water samples; conservation the samples and preparing thereof for following analysis; cultivating test-organism, namely one-celled green algae - Chlorella vulgaris, inside cultivator at temperature of 36±0.5°; measuring optical density thereof in red light; analyzing and estimating the measurement results. The observation points are transversal to river and set in front of single sewage pollution source or sewage pollution source array, in center of each source and behind them. All three observation points are located within the limits of a city or other inhabited locality. The optical density is measured before and after one-celled green algae cultivation in water samples. After measurement termination overall river water pollution index is determined.

EFFECT: possibility to compare overall river water pollution index obtained in particular observation point with that obtained from pollution source on river bank; extended functional capabilities and extended range of application.

5 cl, 4 tbl

FIELD: ecology.

SUBSTANCE: the present innovation deals with testing biological activity of water, preliminary treatment of water, division into control and tested portions, ionization of tested portion with silver ions, detection of the quantity of sprouted wheat grains per time unit pre-impregnated in both mentioned portions and calculation of relative alteration for the value of biological activity of water according to the following ratio: where d - relative alteration for the value of biological activity of water, %; Ntested - the quantity of sprouted grains per time unit in tested portion of water, pcs.; Ncontrol - the quantity of sprouted grains per time unit in control portion of water, pcs. Moreover, relative alteration for the value of biological activity of water being above 0 means increased biological activity of water, relative alteration for the value of biological activity of water being below 0 means its decrease, and equation of the mentioned relative alteration to 0 means intact nature of biological activity of water being different by the fact that pre-treatment of water should be carried out due to precipitation for 23-24 h at 23-26 C.

EFFECT: higher efficiency of investigation.

1 tbl

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