Differential diagnostic technique for chronic prostatitis
SUBSTANCE: patient's prostate secretion microscopy and microbiological analysis of sperm are conducted prior to and after three sessions of low-intensity infra-red laser therapy. Further, a final diagnosis of category II, category IIIA or category IIIB chronic prostatitis is stated.
EFFECT: higher differential diagnostic accuracy in chronic prostatitis forms which have essentially different pathogenesis and therapy and improved therapeutic results.
The invention relates to medicine, namely to urology.
Close goal is to offer Kogan M.I. et al. "A method for the diagnosis of chronic bacteriologi prostatitis", 2004 Patent number RU (11) 2272294. The essence of the invention lies in the fact that in the serum to determine the activity of kallikrein and values from 18 to 39 IU/ml diagnose chronic bacterially prostate inflammatory forms, and when values of 39 IU/ml and above diagnosed with chronic abacterial prostatitis noninflammatory forms. The disadvantages of this method is the technical complexity of the method, the need for highly qualified laboratory and the difficulty of reproduction of this method.
Another similar invention is to offer Sarichev A.V. et al. The method of differential diagnosis of chronic bacteriologi prostatitis", 2006 Patent number RU (11) 2315318. The essence of the method: patients in serum to determine the activity of superoxide dismutase (SOD) and catalase (cat). When the decrease of SOD activity from 69 to 62 srvc. units/ml and the increase of catalase activity from 66 to 74 units/ml diagnose chronic abacterial prostatitis (HAP) inflammatory forms. When the activity of SOD from 62 srvc. units/ml and below and the increase of catalase activity from 60 to 66 units/ml - HAP non-inflammatory forms. The disadvantages of this ways is and is the technical complexity of the method, skilled laboratory and the difficulty of reproduction of this method.
Also close method proposed Changeeven A.V. et al., The method of differential diagnosis of chronic bacteriologi prostatitis", 2007 Patent number RU (11) 2359273. The essence of the method: in the blood of the subject determine the content of nitric oxide released macrophage NO-synthase. When the content produced NO faguoqitirute blood cells from 23 [n/M 2·105
cells]·10-3and above diagnosed with chronic abacterial prostatitis (HAP) inflammatory forms, and when the content of 16 [n/M 2·105cells]·10-3and lower - HAP non-inflammatory forms. The disadvantages of this method is the technical complexity of the method, the need for highly qualified laboratory and the difficulty of reproduction of this method.
As a prototype of the selected method of differential diagnosis of chronic bacteriologi prostatitis, which consists in determining the activity level of 2-macroglobulin, based on the values which are diagnosed chronic abacterial prostatitis noninflammatory and inflammatory forms EN 2276359 C1, published 10.05.2006. Our proposed method differs from the prototype in that conduct microscopy prostate secretion jelly is s and microbiological analysis of sperm before and after three sessions of low-intensity infrared laser therapy.
The aim of the proposed method is to improve the accuracy of differential diagnosis of prostatitis II, IIIA and IIIB categories of chronic prostatitis.
This goal is achieved by the fact that twice was performed microscopy of prostate secretion before and after three sessions of low-intensity infrared laser according to the described method. Simultaneously with the analysis of a secret of a prostate gland was carried out bacteriological analysis of sperm.
The technical result of the proposed method is to improve the accuracy of differential diagnosis of chronic prostatitis II, IIIA and IIIB, which have fundamentally different pathogenesis and treatment. This is achieved by conducting a three-day course of low-intensity infrared laser therapy with the subsequent study of prostate secretion and bacteriological analysis of sperm. This survey was conducted in men with chronic prostatitis category IIIB.
We used low-intensity infrared laser "ALT Mustang 2000" and "ALT Mustang 2000-2" with penetrating power of about 6-8 cm
The technique of laser therapy: the duration of exposure of 1 minute for each of the zones. Zone: above the fold - 600 Hz; lumbar region - 300 Hz; crotch - 80 Hz; the area of the femoral artery - 80 Hz (Semenov A.V. et al. Low-energy lacerater the Pius in the complex treatment of chronic prostatitis. // The Plenum of the Board of VNO: proc. Dokl. - Perm. - 1994. - S-153). After conducting a three-day course of therapy was conducted microbiological semen analysis and microscopy of prostate secretion by traditional methods and bacteriological analysis. It was estimated the same indicators as before laser therapy.
The study was conducted on the basis of the urological clinic of the GOU VPO Yugma University by G. Ivanov.
The study involved men who applied for chronic prostatitis, the examination of which were diagnosed: chronic prostatitis category IIIB. The diagnosis of chronic prostatitis was also confirmed by the data of the transrectal us of the prostate gland, bacteriological examination of prostate secretion of growth of microorganisms have been identified, a blood test for PSA was within the age norm. All patients received laser therapy according to the described method, then re-performed microscopy secret of the prostate gland. The proposed method allows to obtain objective criteria for antibacterial therapy. The basis of diagnosis was the classification proposed in 1995 by the national Institute of health, USA (J.N. Krieger, L. Nyberg, Jr., Nickel J.C. NIH consensus definition and classification of prostatitis // J.A.M.A. - 1999 - Vol.282. - P.236-237). Category II - chronic bacterial prostatitis condition is yavlyaetsya upon detection of pathogenic bacteria 10 3and more microbiological research of a secret of a prostate, a third portion of urine after prostate massage or ejaculate.
Category IIIA - inflammatory chronic pelvic pain syndrome more than 3 months, the presence of more than 10 leukocytes per field of view in the prostate secretion or urine after prostate massage; no or less than 103pathogenic bacteria in samples of prostate secretion, the third portion of urine after prostate massage or ejaculate.
Category IIIB - noninflammatory chronic pelvic pain syndrome more than 3 months, less than 10 leukocytes per field of view in the prostate secretion or urine after prostate massage; no or less than 103pathogenic bacteria in samples of prostate secretion, the third portion of urine after prostate massage or ejaculate.
Initially, all patients fit the criteria for chronic prostatitis category IIIB, which is shown as one of the treatments of low-intensity infrared laser therapy. After three sessions of low-intensity infrared laser therapy they have been re-examination and according to the examples in part of the patients were identified criteria matching the other categories, and accordingly the newly established category of chronic prostatitis them was changed treatment.
The laser therapy popisannoe technique in healthy volunteers inflammatory changes in the analysis of a secret of a prostate gland is not provoked.
Thus, this technique helps to solve the issues of further tactics of examination, treatment, prognosis.
The proposed method allows to obtain objective criteria for antibacterial therapy. The basis of diagnosis was the classification proposed in 1995 by the national Institute of health, USA (J.N. Krieger, L. Nyberg, Jr., Nickel J.C. NIH consensus definition and classification of prostatitis // J.A.M.A. - 1999 - Vol.282. - P.236-237).
Clinical example 1
Patient, 30 years. Went to the clinic with complaints of pain in the perineum, radiating to the lower abdomen, testicles, increasing during intercourse, nocturia 1-2 times per night, increased urination. According to the transrectal us: diffuse heterogeneous echostructure of the prostate contour fuzzy. In the analysis of a secret of a prostate: leukocytes 8-10 in the field of view, the epithelium +; lecithin grain +++; flora is not revealed. In the bacteriological examination of sperm Staphylococcus epidermidis growth of the cumulative environment.
Held laser according to the described method three times. Comparative analysis of a secret of a prostate: leukocytes 15-20-25; epithelium +; lecithin grain ++; flora +. Control bacteriological semen analysis: E. coli in the titer of 5 x 105CFU/ml
Thus, a patient diagnosed with chronic prostatitis category II, assigned to the anti-inflammatory and antibacterial therapy.>
Clinical example 2
Ill "L", 49 years old. Went to the clinic with complaints of increased urination, low-intensity pain in the perineum, radiating to the testicles. According to the transrectal us: diffuse heterogeneous echostructure of the prostate gland, calcium, fuzzy contour. In the analysis of a secret of a prostate: leukocytes 2-6 in the field of view, the epithelium ++; lecithin grain +; flora is not revealed. In the bacteriological examination of sperm growth of microflora was not detected.
Held laser according to the described method three times. Comparative analysis of a secret of a prostate: leukocytes 18-24-28, in clusters of up to 40; the epithelium +; lecithin grain +++; flora is not revealed. Control bacteriological semen analysis: Staphylococcus saprophyticus in titer of 1*102CFU/ml
Thus, a patient diagnosed with chronic prostatitis category IIIA assigned to anti-inflammatory and antibacterial therapy.
Clinical example 3
The patient "M", 34 years. Went to the clinic complaining of intense pain in the perineum, radiating to the lower abdomen, testicles, sacral region. According to the transrectal us: diffuse heterogeneous echostructure of the prostate gland due to alternating areas of high and normal ahopelto, fuzzy contour. In the analysis of a secret of a prostate: leukocytes 1-2 in sight, the epithelium +; lecithin grain +++; flora not Yavlena. In the bacteriological examination of sperm growth of microflora is not revealed. A blood test for PSA - 0.02 ng/ml.
Held laser according to the described method three times. Comparative analysis of a secret of a prostate: leukocytes 1-2; epithelium +; lecithin grain ++; flora is not revealed. Control bacteriological semen analysis: the growth of microorganisms are not detected.
Thus, a patient diagnosed with chronic prostatitis category IIIB, antibacterial therapy is not shown.
The method of differential diagnosis of chronic prostatitis, including laboratory studies, characterized in that for confirmation of the diagnosis patients perform microscopy of prostate secretion and microbiological analysis of sperm before and after three sessions of low-intensity infrared laser therapy, and then finally establish the diagnosis of chronic prostatitis category II, category IIIA or category IIIB.
SUBSTANCE: in patients with bronchial asthma performed is iionophoresis with 5% acetylcholine solution in course of laser Doppler fluometry, time of rise and restoration of dopplerogramme on skin of left forearm in point located on medial line 4 cm higher than base of styloid processes of ulnar and radial bones.
EFFECT: basing on obtained data type of microvascular endothelium response is determined as normoreactive-stable, normoreactive-incremental, normoreactive-decremental, hyperreactive-stable, hypereactive-incremental, hypereactive-decremental, hyporeactive-stable, hyporeactive-decremental ot hyporeactive-decremental type of microvascular endothelium response.
10 dwg, 9 ex
SUBSTANCE: reagent for thrombocyte measurement containing Nile blue hydrosulphate as a reagent for thrombocyte staining. A reagents kit for thrombocyte measurement containing Nile blue hydrosulphate as a first reagent, and a buffer solution as a second reagent. A method for thrombocyte measurement wherein Nile blue hydrosulphate is used as the first reagent, and the buffer solution - as the second reagent. A reagent for thrombocyte measurement containing Nile blue and an acid as a staining agent for thrombocyte staining. The reagents kit for thrombocyte measurement containing Nile blue as the first reagent, and a buffer substance as the second reagent. The method for thrombocyte measurement wherein Nile blue and the acid are used as the first reagent, and the buffer substance - as the second reagent.
EFFECT: reagents described above provides higher accuracy of thrombocyte evaluation.
13 cl, 15 dwg, 10 tbl, 48 ex
SUBSTANCE: histologic examination of transverse section is performed through all the layers of arachnoid cyst walls with previous alignment of the piece in paraffin on the edge and study the state of lining and connective-tissue membrane. In case the wall of the cyst is covered in flat arachnoendothelium with hyperchromatic nucleus, the cyst is regarded the non-proliferating, the prognosis is favorable. In case the wall of the cyst is covered with round-shaped arachnoidendothelium with the zones of amplified proliferation of arachnoidendothelium in form of multirowed layer and/or vertical cells alignment, it is regarded as proliferating - the prognosis is unfavorable.
EFFECT: morphologic prediction of children arachnoid brain cyst progression.
1 dwg, 2 dwg, 1 tbl, 2 ex
SUBSTANCE: invention relates to field of medicine, namely to hepathology and pediatrics. In order to predict exacerbations of chronic viral hepatitis B in teenagers, blood test is performed. By means of bioluminescent method determined is activity of lymphocyte enzymes in peripheral blood of patients: glucose-6-phosphate dehydrogenase (G6PDG) and lactate dehydrogenase (NADN-LDG), ratio of activities is calculated. If value of obtained index is equal or is higher than 1.15 exacerbations are predicted, if index value is lower than 1.15, absence of exacerbations is predicted.
EFFECT: method makes it possible to predict exacerbations of chronic viral hepatitis B in teenagers with clinical-biochemical remission with high accuracy.
4 ex, 2 tbl
SUBSTANCE: formation of a risk group of neoplastic disorders in a cervical epithelium is carried out by IF staining of a cervical epithelium smear with using a monoclonal antibody reacting with a sialylated conformational glycopeptide antigen determinant as a part of MUC1 followed by with cell finish staining with chromogenic nuclear stain. The sample is examined by alternated fluorescent light and transmission light. Each cell with a depolarised stained surface membrane detected by fluorescent light is examined in transmission light. If the cells with the depolarised stained surface membrane detected by fluorescent light show cytological symptoms of dyscariosis, and/or proliferative parabasal cell complexes with no symptoms of dyscariosis show depolarised stained surface membranes or granular stained cytoplasm, a patient is subsumed to the risk group of the presence of neoplastic disorders in the cervical epithelium.
EFFECT: higher analysis accuracy.
SUBSTANCE: LAKK-02 apparatus is used to evaluate a capillary blood flow reserve in two iontophoresis sampled taken from skin of a left forearm in a point along a median line 4 cm higher than styloid process of ulna and radius. An endothelial function coefficient EFC is calculated by formula: where AC BFR is the capillary blood flow reserve in response to acertylcholine; SN BFR is the capillary blood flow reserve in response to sodium nitroprusside. If the EFC value is lower than 1.0, a disturbed microvascular endothelium vasoregulation is stated.
EFFECT: use of the invention allows simplifying diagnostics of the microvascular endothelium status in patients with bronchial asthma.
SUBSTANCE: biopsy materials of a mucous membrane of a gastric corpus and a duodenum are analysed for mast cell count per 100 cells of infiltrate. At detecting more than 3.5 mast cells per 100 infiltrate cells in the biopsy materials of the mucous membrane of the gastric corpus and more than 4.75 cells per 100 infiltrate cells in the biopsy materials of the mucous membrane of the descending duodenum, immunohistochemistry or PCR diagnostics are required. If detecting Epstein-Barr virus antigens or DNA in the biopsy materials of the gastric and duodenal mucosa, EBV associated gastroduodenitis is diagnosed.
EFFECT: use of the technique allows diagnosing EBV associated gastroduodenitis in children.
SUBSTANCE: invention describes method of noninvasive potentiometric determination of oxidant/antioxidant activity of biological tissues, which includes bringing analysed object in contact with electroconductive medium, which containing mediator system and estimation of oxidant/antioxidant activity by change of potential difference on electrodes, introduced into electroconductive medium, and electroconductive medium represents gel, which contains as mediator system a pair of chemical compounds, containing element in different degrees of oxidation, electrodes through gel contact with the analysed object, and oxidant/antioxidant activity is determined by formulae. For realisation of method claimed is device, which includes apparatus for measuring potential difference and filled with electroconductive medium reservoir with working electrode and electrode of comparison, connected with device for measuring potential difference, where reservoir is made open from one side. As electroconductive medium applied is gel, and working electrode is made in form of plate, placed from the side of open part of reservoir and partially covering it.
EFFECT: increase of reliability and accuracy of obtained results.
4 cl, 5 ex, 6 dwg
SUBSTANCE: light flux power passed though biological tissue specimen is measured in photometric chamber at two different flux reception aperture. Note here that, first, diaphragm is used to limit flow diameter to that equal to diameter d of specimen illuminating beam. Then, said diameter is increased to diameter D 5 to 10 times greater than first diameter d. Dissipation and absorption factors s and a are defined from formulas, respectively: s = p∂+τ∂ and a = 1 - s - τd, where τd is directed transmission factor defined as τd=Fτd/F0, F0 is initial power of illuminating beam, (mW), Fτd is measured power of transmitted flux with diaphragm d, τ∂ is diffusion transmission factor defines as: τ∂ =τ -τd, where τ is total (integral) transmission factor defined as τ=Fτ/F0, where Fτ is measured power of transmitted flux, (mW), with diaphragm D, ρ∂ is back scatter factor defined from for photometric invariant
EFFECT: high accuracy.
1 ex, 1 dwg
SUBSTANCE: stool consistency and urine acidity are determined. Stool type is estimated by Bristol scale. Diet is administered. When stool type 5 or 6 by Bristol scale and alkaline urine reaction are achieved, conclusion about sufficient content of dietary fibres in diet is made.
EFFECT: method makes it possible to establish individual need in dietary fibres.
SUBSTANCE: method involves examining blood erythrocytes. Blood erythrocyte suspension fluorescence intensity is measured at wavelength of 500 nm polarized along light axis and then at wavelength of 493 nm in perpendicular to the light axis for each polarization direction with following anisotropy value being calculated. Its deviation from norm proves availability of hemolytic disease.
EFFECT: accelerated examination process; reduced risk of traumatic injuries of fetus or newborn.
FIELD: veterinary medicine.
SUBSTANCE: method involves carrying out blood erythrocyte micro electrophoresis in alternating electric field with sign change frequency equal to 0.3-1 Hz. Electric field having current intensity equal to 4.5-5.0 mA is applied. Treatment is applied at 36-38°C. Oscillation amplitude being equal to 3 mm and less, endotoxicosis is detected.
EFFECT: high accuracy and simplicity of the method.
SUBSTANCE: method involves applying integrated diagnostics approach with blood serum examination being applied using infrared spectrometry methods. Sample absorption spectra in the area of 1200-1000 cm-1 are recorded, absorption peak heights having maximum at 1170, 1165, 1140, 1125, 110, 1070, 1025 cm-1 points are measured. Then, mean peak height C and ratio X of peak height at 1125 cm-1 point of maximum to C are calculated. X≥0.7 being the case, light myocardium injury degree is to be diagnosed. 0.7>X≥0.53 being the case, moderate severity degree case is to be diagnosed. X<0.53 indicates grave injury to take place.
EFFECT: high reliability of diagnosis.
FIELD: analytical methods in medicine.
SUBSTANCE: cells of tested organs are subjected to alternating cytological electrophoresis. When cell vibration amplitude rises by at least 20%, drug is regarded to be appropriate for treatment.
EFFECT: increased drug selection efficiency.
FIELD: medicine, hepatology.
SUBSTANCE: the present innovation deals with detecting bilirubin content both before and after curative starvation, moreover, curative starvation one should introduce antipyrine at the dosage of 1 mg/kg, in a day it is necessary to detect antipyrine content in saliva and urine and bilirubin content and at bilirubin content being 30-40 mM/l one should starve at daily intake of 400-450 kcal/d for 3 d, then one should again detect bilirubin level and at its increase by 50-100% one should again introduce antipyrine at the dosage of 1 mg/kg and according to its delayed half-life by 20-50% it is possible to diagnose Gilbert's disease.
EFFECT: higher accuracy of diagnostics.
FIELD: medicine, otorhinolaryngology.
SUBSTANCE: the present innovation deals with treating diseases of the upper respiratory tract. One should detect disorders of mucociliary transport (MCT) due to measuring the motor parameters of ciliated epithelium cilia, at least, the rate of mucociliary transport that includes material sampling for further testing as ciliary epithelial cells and registration of cilia fluctuations in human respiratory tract due to TV microscopy performed in patient's lifetime. Material for testing should be sampled from mucosal surface of respiratory tract, and therapeutic tactics should be chosen depending upon motor velocity of cilium's tip obtained based upon mathematical modeling followed by calculations by the formula: , where Vc - velocity of cilium's tip [mcm*sec1]; C1, C2, α - calculated as average values being characteristic for every of human body states under investigation: healthy persons, patients with either acute or chronic forms of sinusitis by the developed model of cilia movement; C1 - curvature of cilium's working length, constant being equal to 1/38 mcm-1; C2 - cilium's curvature from its working length up to the tip [mcm-1]; α - cilium's inclination angle against vertical position [degrees]; π = 3.1417 - constant; L, Ts and Te - parameters measured individually in every patient; L - cilium's length [mcm]; Ts - time from the onset of movement till maximal straightening of cilium's tip [sec]; Te - time of efficient impact [sec]. Moreover, the value being Vc = 9.6 - 11.3 mm/min corresponds to normal MCT functioning. For patients with chronic purulent sinusitis beyond exacerbation accompanied with MCT disorder it is necessary to prescribe complex therapy: surgical or conservative therapy with preparations of secretolytic and secretomotory action. For patients with chronic purulent sinusitis beyond exacerbation without any MCT disorder one should prescribe surgical therapy. All patients with acute sinusitis with MCT disorder should be prescribed complex conservative therapy with preparations of secretolytic and secretomotory action. The method enables to specify optimal therapy based upon scientifically proved indications of investigations by taking into account individual peculiarities of every patient.
EFFECT: higher efficiency.
5 dwg, 3 ex, 2 tbl
FIELD: medical engineering.
SUBSTANCE: device has non-absorbing substrate having hydrophilic target region, which is covered with reagent by applying non-impact micro-drop printing method to produce practically uniform reagent layer. The device is in particular usable for measuring blood coagulation time. Preferential invention embodiment involves determining blood coagulation time by carrying out monitoring of light transition through the target region as the blood sample cover interacts with the reagent.
EFFECT: high reliability of analysis results.
20 cl, 9 dwg
SUBSTANCE: one should register the values of pH and Redox potential (Red) of liquid means in bio-objects simultaneously. It is necessary to plot a graph ▵pH/▵Red according to fluctuations of variation values in measured parameters at their temporal diagram to transfer it into a columnar one where the height of every column is proportional to the area of this graph between neighboring measurements, correspondingly. According to the ratio of area sums of positive columns to that of negative ones during a certain period of time ( from a minute- to a year-long ones) one should conclude upon a state in a bio-object. The value ranged 0.5-2 is considered to be a standard. The method enables to provide efficient control for a two-phase systemic process.
EFFECT: higher efficiency and accuracy of diagnostics.
1 cl, 9 dwg, 5 ex, 1 tbl
FIELD: medicine, clinical neurology, neurosurgery.
SUBSTANCE: one should perform crystallographic study if cerebrospinal fluid and at protein concentration in liquor samples being from 0.50 g/l and higher at predominance of crystals as suppressed dendrites upon a crystallographic picture one should diagnose a malignant cerebral tumor, and at protein concentration being below 0.50 g/l and predominance of crystals as branched dendrites - a benign cerebral tumor. The innovation enables to fulfill not only due diagnostics of cerebral neoplasms, but detect the degree of tumor process malignancy at the early stages of its development.
EFFECT: higher accuracy of differential diagnostics.
3 dwg, 2 ex, 1 tbl
FIELD: biology, medicine, in particular histological investigation of shells with natural surface by using light-optical microscope.
SUBSTANCE: claimed method includes surface relief investigation by using plane field microscope in reflected light at one-side falling shadow-forming lighting. Light-reflecting ability of specimen surface is provided by silver impregnation. Said impregnation is carried out by specimen hold in 10 % silver nitrogen solution for 10 min followed by reducing thereof with 1 % ascorbic acid solution for 1 min.
EFFECT: method for investigation of natural shell surface relief of improved quality due to visual representation of surface three-dimension image on tissue level.
3 cl, 4 dwg