Method of estimating microbial risk of development of bacterial intestinal infections transmitted by water way or calculation of microbial risk level

FIELD: medicine.

SUBSTANCE: isolated during bacteriologic analysis of quality of water for various purposes pathogenic bacteria, additionally potentially pathogenic bacteria with their further identification and normalised indicators of water quality estimation are taken into considereation with further implementation of process of generalisation into a single assessement of microbial risk thresholds, which is performed by calculating first weighted coefficients by formula: An=(P1·V1+P2·V2+…+Pn·Vn). After that, the data obtained are used to calculate integral index by each sanitary and hygienic factor by formula: Estimation of risk by three-level classifier is considered high if values of Rfac are from 0.4 to 1.0 including 0.4 and 1.0, medium if values of Rfac are from 0.2 to 0.4 and low if values of Rfac are from 0 to 0.2, including 0. Estimation of risk by five-level classifier is considered to be very high if values of Rfac are from 0.6 to 1.0, including 0.6 and 1.0, high if values of Rfac are from 0.4 to 0.6, including 0.4, medium if values of Rfac are from 0.2 to 0.4, including 0.2, low or acceptable if values of Rfac are from 0.15 to 0.2, including 0.15 and very low if values of Rfac are from 0 to 0.15. After that the obtained results are used to calculate generalised index of risk of sexually transmitted bacterial intestinal infections CA by formula by three-level classifier it is considered high if values of CA are from 0.4 to 1.0, including 0.4 and 1.0, medium if values of CA are from 0.2 to 0.4, and low if values of CA are from 0 to 0.2, including 0. Estimation of risk by five-level classifier is considered very high if values of CA are from 0.6 to 1.0, high if values of CA are from 0.4 to 0.6, including 0.4, medium if values of CA are from 0.2 to 0.4, including 0.2, low or acceptable if values of CA are from 0.15 to 0.2, including 0.15 and very low if values of CA are from 0 to 0,15.

EFFECT: application of claimed method makes it possible to reduce or prevent morbidity associated with bacterial semination of water.

1 ex, 8 tbl, 1 dwg

 

The present invention relates to medicine, more specifically to work on the epidemiological assessment of the sanitary and hygienic conditions in order to prevent intestinal infections that are transmitted through water.

In modern conditions the health of the nation is largely determined by its sanitary and epidemic well-being, one of the most important factors is the creation of a secure environment water population.

Evaluation of microbial health risks is one of the elements of the methodology of risk analysis includes risk assessment, risk management and reporting risk. In scientific terms, the health risk assessment is a consistent, systematic review of all aspects of the analyzed factors on human health, including the rationale for allowable exposure levels. In scientific-practical application the main objective of risk assessment is to obtain and compile information on the possible influence of factors of environment on the state of his health, is necessary and sufficient for hygienic substantiation of optimal management decisions to eliminate and reduce risk, optimize control (control and monitoring) level exposures and risks.

Risk assessment for human health is a quantitative and/or qualitative characteristics of the Reden effects able to develop as a result of influence of factors of environment on a specific group of people under specific exposure conditions.

On the epidemic process in intestinal infections that are transmitted through water, is influenced by many factors of natural, biological and social nature. This social-hygienic factors are largely determinative. Violation of the design standards water supply and water, poor sanitation and improvement of settlements, inadequate disinfection of drinking water and wastewater can be crucial in the emergence and spread of acute intestinal infections.

The study of outbreak and sporadic incidence of intestinal infections transmitted by water conducted by the authorities of the sanitary epidemiological service, descriptive, that it is not possible to establish a quantitative relationship between sanitation and disease OKA. This is due to the lack of a unified mathematical methods of systematization and generalization of sanitary data in quantitative terms. In addition, inadequate assessment of the risk of microbial contamination of drinking water does not allow to obtain reliable results for their Messiah. statisticheskoi significance and degree of approximation to the actual size of the microbial risk assessment.

The closest analogue of the claimed method from the prior art is not revealed.

The technical purpose of this invention is the reduction or prevention of disease associated with microbial contamination of water by using the proposed mathematical model, many qualitative features with reliable probability forecast.

The technical result is achieved by a method for assessing the microbial risk of bacterial intestinal infections, waterborne, or the calculation of the level of microbial risk, taking into account selected when conducting bacteriological analysis of water quality for various purposes pathogenic bacteria, additionally potentially pathogenic bacteria and their subsequent identification and standardized indicators of water quality assessment and subsequent implementation of the process of consolidating all of the indicators and factors into a single score thresholds microbial risk, carried out by calculating the first weighted according to the formula:

An=(P1·V1+P2·V2+...+Pn·Vn),

where

Andn- weighted factor for determining the relative index; Rn- calculated the likelihood of intestinal diseases for each relative indicator; Vn- weight of the i - poryadke the new number of indicators, n is the number of parameters, then the data obtained by weighted indicators are calculated integral indicator for each health factor by the formula:

where N is the number of indicators used in the assessment of sanitary conditions affecting the quality of drinking water; i - ordinal indicator; Vn- the weighting factor of the i-th metric; Pn- calculated the likelihood of intestinal diseases for each relative indicator; W is the sum of the weights, then the obtained results with all hygiene factors calculate the generalized risk of bacterial infections that are transmitted by water according to the formula:

where N is the number of factors used in the computation of the generalized indicator of the level of microbial risk; i is the number factor; G - weight coefficient; Rfac- the integral indicator of risk for each hygiene factor; W is the sum of the weights on the basis of the generalized parameter by comparing with a threshold value to identify the level of risk of bacterial disease that spreads through water

The proposed method contains the basic risk factors: the degree and x is the nature of microbial contamination of sources of centralized drinking water supply centralized drinking water supply; the centralized water supply sources; household water use population and recreation; municipal landscaping and comprehensive assessment of the sanitary-hygienic conditions of use (these figures may vary):

to explain the contribution of each individual indicator in the overall risk assessment for each health factor to determine a generalized risk assessment on all health and safety factors as a whole;

- to predict epidemic situation on the basis of generalized data, taking into account the integral and generalized measures of risk;

- to identify the environmental factors that promote or inhibit the emergence and spread of intestinal infections caused by water transfer, because in each locality may effect one or a specific group factors;

to give a comprehensive evaluation of the generalized indicator of microbial risk with all sanitary and hygienic conditions in the locality;

on the basis of the received data to develop measures to eliminate or best possible mitigation of adverse factors;

- prioritize measures to improve sanitary and hygienic conditions of water use in concr the coherent settlement in the prevention of intestinal infections, due to water transfer factor;

- to assess the degree of microbial risk of acute intestinal infections and the possibility of implementing the water route of transmission.

The proposed method is implemented as follows.

Water quality assessment is carried out according to the current legislation for a particular type of water use: 1) for centralized drinking water supply taking into account the level of bacterial contamination of drinking water content in the water of pathogenic bacteria in accordance with SanPiN 2.1.4.1074-01 (Vibrio cholerae 01 group, Campilobacter jejuni, E. coli O 157:H7, Salmonella typhi, Salmonella paratiphi a and b, Shigella, Legionella pneumoniae, Yersinia pseudotubaerculesis) and potentially pathogenic (Klebsiella, Pseudomonaaeruginosa, Proteus, Enterobacter, Citrobacter and other bacteria of the family Enterobacteriaceae, identified by the international scheme typing), the intensity of pollution indicators of water supply of the population, sanitary and technical condition of the distribution network, regularity of water supply to the population; 2) for sources of centralized drinking water supply - determine the level of bacterial contamination in source water before and after disinfection, the presence of zones of sanitary protection (in accordance with SanPiN 2.1.4.1074-01, SanPiN 4.2.1884-04, SanPiN 2.1.4.117502); 3) for centralized water supply sources is given which indicate the level and intensity of bacterial contamination of water, the compliance of the source with the requirements of SanPiN 2.1.4.1175-02; 4) conditions for domestic water use population and recreational areas take into account the extent of public use of water from surface water bodies for domestic needs, identifying the frequency of samples exceeding the permissible level, and the intensity of pollution in bathing sites, and detection in water of infectious diseases (Vibrio cholerae 01 group, Campilobacter jejuni, E. coli O 157:H7, Salmonella typhi, Salmonella paratiphi A and B, Shigella, Legionella pneumoniae, Yersinia pseudotubaerculesis) and potentially pathogenic bacteria (Klebsiella, Pseudomonaaeruginosa, Proteus, Enterobacter Citrobacter and other bacteria of the family Enterobacteriaceae, identified by the international scheme typing), the impact of releases of domestic wastewater in the area of water use, etc. in accordance with SanPiN 2.1.5.980-00); 5) for community improvement of settlement is the estimation of water supply and sanitation housing in the municipal and private sectors, the development of water supply and Sewerage network.

For calculation of the generalized indicator of microbial risk is to use weighting factors Gibased on the significance of this factor in integrated assessment: for centralized drinking water supply G1=1,0; for centralised Ho is austeno drinking water supply G 2=0,7; for non-centralized drinking water supply G3or =0.6; for household water use on sanitary and bacteriological indicators G4=0,8; to assess the impact of community beautification G5=0,7.

The proposed method is illustrated by examples.

Example 1.

The calculation of the weighted evaluation of microbial risk associated with the conditions of centralized drinking water supply mattakuliya in 1986 (table 1-7). In mattakuliya investigated centralized drinking water supply (see table 1). Table 2-6 provides for the establishment of the weighting factor and analytical dependencies of each estimate. Table 7 - example of calculation of each monthly indicator for mattakuliya in drinking water of the population.

The numerical value of each monthly figure was compared in table 2-6 the value for the appropriate use indicator Pnand set the weighting factor. Then according to the formula (1) was calculated weighting factor for each indicator (table 7).

On the basis of the calculated values of R (for integral evaluation of microbial risk is Rfacwhen conducting a comprehensive assessment of microbial p is ska - this isAdue to conditions of water use, determine the assessment of microbial risk of bacterial intestinal infections transmitted by water (or the level of microbial risk) (table 8).

The evaluation of the generalized indicator of risk of diarrhoeal infections, disseminated by water, is illustrated in the graph (figure 1). The graph shows the estimated generalized indicator of the risk assessment mattakuliya (1), the real incidence per 10 thousand population (2) and thresholds of risk levels on the five-level and three-level classifiers (3). When comparing calculation of the generalized metric and level of real morbidity mattakuliya the confidence level of the forecast is from 75 to 95%.

The data demonstrate the prospects of using mathematical models of risk assessment depending on the state sanitary-hygienic conditions of water use and real bacterial load (with confidence levels of forecast p<0,01-0,05), which allows you to give in-depth obobshenno the assessment of the bacterial load caused by water.

Thus, the developed method for assessing the microbial risk allows us not only to predict changes of sanitary and epidemiological situation, but also to assess the contribution of each factor which is scored these changes are due, to justify control measures, ensuring the safety of water use. Evaluation criteria each of the considered sanitary and bacteriological parameters (for three-level and five-level microbial risk assessment) provide an opportunity to evaluate the result of the evaluation by the extent of their likely impact on epidemic risk of bacterial enteric infections. The analytical dependences for each indicator of water quality, to bring its actual value in a probability value, which changes in the risk range from 0 to 1.

The proposed method for the assessment of microbial risk allows on the basis of objective indicators to identify deficiencies in the system of municipal water supply points: water-treatment systems, the efficiency of cleaning and disinfection of water supply networks, as well as to determine the degree of potential epidemic hazard of spread of bacterial intestinal infections, water-borne, which will allow you to prioritize certain measures to improve sanitary and hygienic conditions of water use and reduction of acute intestinal infections caused by a water factor of their gear.

Table 1.
Balanced assessment of microbial risk associated with centralized drinking water supply to the population mattakuliya.
IndicatorsMonths (X)
SeptemberOctoberNovemberDecemberJanuary
The percentage of non-compliant samples of the water before it enters the distribution network
The percentage of non-compliant samples of water in the distribution network.
The average index of the Bureau in the distribution network(CFU/100 ml).
Percentage of water samples in the distribution network with the index of the Bureau for more than 20
TBC in 1 ml of water distribution networks
Detection of pathogenic bacteria (%)0000
The integral indicator Rfac035 0,280,150,450,03
Risk assessment for three levels classifier0.2≤0.35<04
R_2
High
0.2≤0.28<04
R_2
High
0≤0.15<0.2 R_1
Acceptable
0.4≤0.45<1.0
R_3 High
0≤0.03<0.2
R_1 Acceptable
Risk assessment for maturalnego classifier0.2≤0.35<0.4 R_3
High
0.2≤0.28<0.4
R_3
High
0.15≤0.15<0.20
R_2
Acceptable
0.4≤0.45<0.80
R_4
High
0≤0.03<0.15
R_1
Low
*the numerator is the absolute value of the index
** the denominator is the value of the weighting factor.

Table 7.
An example of the calculation of the weighted evaluation of microbial risk associated with the conditions of centralized drinking water supply mattakuliya.
IndicatorsThe calculation of the weighted values by month
SeptemberOctoberNovemberDecemberJanuary
The percentage of non-compliant samples of the water before it enters the distribution networkIf 0<2,
then R15=0:2=00<20<20<20<2
When V=0,9P15=0:2=0P15=0:2=0P15=0:2=0P15=0:2=0
A=0·0,9=0V=0,9V=0,9V=0,9V=0,9
(Similar races the em is for other metric) 0·0,9=00·0,9=00·0,9=00·0,9=0
The percentage of non-compliant samples of water in the distribution network.6,45<1514,29<154,6<155,88<150<15
P16=6,45:15=0,43P16=14,19:15=0,94P16=0,31:15=0,31P16=5,88:15=0,39P16=0:15=0
V=0,7V=0,7V=0,7V=0,7V=0,7
0,7·0,43=0,300,7·0,94=0,660,7·0,31=0,210,7·0,39=0,270,7·0=0
The average index of the Bureau in the distribution network (CFU/100 ml).21,33>154,18<154,6<153,22<15a 3.0<15
P17=1P17=4,18:15=0,28 17=4,6:15=0,31P17=3,22:15=0,21P17=3,0:15=0,2
V=0,7V=0,7V=0,7V=0,7V=0,7
0,7·1=0,70,7 x 0.28=0,20,7·0,31=0,200,7·0,21=0,150,7·0,2=0,14
Percentage of water samples in the distribution network with the index of the Bureau for more than 204,3<52,04<52,3<50<50<5
P18=4,3:5=0,86P18=2,04:5=0,41P18=2,3:5=0,46P18=0:5=0P18=0:5=0
V=0,8V=0,8V=0,8V=0,8V=0,8
0,8·0,86=0,690,8·0,41=0,330,8·0,46=0,370,8·0=00,8·0=0
TBC in 1 ml of water in Raspredelitelnaya 19,33<4029,43<406,14<404,73<402,72<40
P19=19,33:40=0,48P19=29,43:40=0,74P19=6,14:40=0,15P19=4,73:40=0,12P19=2,72:40=0,07
V=0,5V=0,5V=0,5V=0,5V=0,5
0,5·0,48=0,240,5·0,74=0,370,5·0,15=0,080,5·0,12=0,060,5·0,07=0,03
Detection of pathogenic bacteria (%).0=00=00=01>00=0
P20=0P20=0P20=0P20=1P20=0
V=2V=2V=2V=2V=2
2·0=02·0=02·0=02·1=22·0=0

Table 8.
Classification of levels of microbial risk
Five-level classifierThree-level classifier
The calculated value of the indexThe level of microbial riskThe calculated value of the indexThe level of microbial risk
0≤R*<0.15Very low-
0.15≤R < 0.20Low (acceptable)0≤R<0.2Low (acceptable)
0.20≤R<0.40Average0.2≤R < 0.4Average
0.40≤R < 0.60High0.4≤R<1.0High
0.60≤R<1.0Very high-
Note: *R - levels of microbial risk assessment for integral parameters (Rfac) and generalized indicators (WithAnd).

Method of assessment microbial risk of bacterial intestinal infections, waterborne, including the consideration allocated in the bacteriological analysis of water quality for various purposes pathogenic bacteria, additionally potentially pathogenic bacteria and their subsequent identification and standardized indicators of water quality assessment and subsequent implementation of the process of consolidating all of the indicators and factors into a single score thresholds microbial risk, which is carried out by calculating the first weighted according to the formula: An=(P1·V1+P2·V2+...+Pn·Vn),
where An- weighted factor for determining the relative index; Pn- the likelihood of intestinal diseases for each relative indicator; Vn- weight coefficient; i is the number of parameter; n is the number of parameters, then the data and calculate the integral indicator for each health factor by the formula: ,
where n is the number of indicators used in the assessment of sanitary conditions affecting the quality of drinking water; i - ordinal indicator; Vn- the weighting factor of the i-th metric; Pn- the likelihood of intestinal diseases for each relative indicator; W is the sum of the weights, assessing microbial risk of bacterial intestinal infections, waterborne, or the level of microbial risk integral indicators on a three-level classifier is considered high when the values of Rfacfrom 0.4 to 1.0, including the 0.4 and 1.0, the average when the value of Rfacfrom 0.2 to 0.4 and low values of Rfacfrom 0 to 0.2, including 0, assessing microbial risk of bacterial intestinal infections, waterborne, or the level of microbial risk integral indicators for batirovna classifier is considered to be very high when the values of Rfacfrom 0.6 to 1.0, including 0,6 and 1,0, high when the values of Rfacfrom 0.4 to 0.6, including 0,4 average at values of Rfacfrom 0.2 to 0.4, including 0,2, low or acceptable at values of Rfacfrom 0.15 to 0.2, including 0.15, and very low when the value of Rfacfrom 0 to 0.15, then the results calculate the composite index of risk of bacterial intestinal infections, waterborne,Andaccording to the formula: ,
where N is the number of factors used in the computation of the generalized indicator of the level of microbial risk; i is the number factor; G is the weighting factor for centralized drinking water supply G1=1, for sources of centralized drinking water supply G2=0,7 for non-centralized drinking water supply G3or =0.6, for household water use on sanitary and bacteriological indicators G4=0,8, to assess the impact of community beautification G5=0,7; Rfac- the integral indicator of risk for each hygiene factor; W is the sum of the weights, assessing microbial risk of bacterial intestinal infections, waterborne, or the level of microbial risk for generalized metrics on three-level classifier is considered high when the values WithAndfrom 0.4 to 1.0, including the 0.4 and 1.0, the average if valueAndfrom 0.2 to 0.4, and low when the values WithAndfrom 0 to 0.2, including 0, assessing microbial risk of bacterial intestinal infections, waterborne, or the level of microbial risk integral indicators for the five-level classifier is considered to be very high when the values WithAndfrom 0.6 to 1.0, including 0,6 and 1,0, high values WithAndfrom 0.4 to 06, including of 0.4, the average values WithAndfrom 0.2 to 0.4, including 0,2, low or acceptable at values of CAfrom 0.15 to 0.2, including 0.15, and very low when the value of CAfrom 0 to 0.15.



 

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3 cl, 10 dwg, 3 tbl

FIELD: chemistry.

SUBSTANCE: invention relates to microbiology. Biological or artificial fluid medium containing the selected microorganism is centrifuged. Supernatant fluid is filtered. A series of diluted samples corresponding to increase in filtrate dilution of up to 10-15 is obtained. The samples are exposed to an electric, magnetic and/or electromagnetic excitation field. Electric signals detected by a solenoid and the digital record of said electric signal after passing through an analogue-to-digital converter are analysed. Diluted samples for which characteristic electric signals, whose amplitude is 1.5 times higher than that of the background noise signals emitted by water, are obtained are selected. Test tubes with equal volume of diluted samples are placed in protective jackets to protect the diluted solutions from external electromagnetic fields. The solution contained in test tube T1 is used as the standard solution. Test tube T2 is placed in the immediate proximity of the sample or is brought into contact with sample X which is presumed to contain the selected microorganism (e.g., E.coli). The obtained electromagnetic signals are compared. Suppression of the signal indicates presence of the microorganism in sample X.

EFFECT: group of inventions enables to obtain reagents and a system for detecting microorganisms in a sample, and detect infection in humans or animals.

3 ex, 8 dwg

FIELD: chemistry.

SUBSTANCE: invention relates to biotechnology and virology. Compositions and methods used to induce immune response against the influenza virus in canines using novel strains, polynucleotides or polypeptides thereof are described. The invention can be used in veterinary.

EFFECT: versions of an influenza virus, which can infect canines and cause a respiratory disease in canines are disclosed.

38 cl, 14 dwg, 25 tbl, 16 ex

FIELD: medicine.

SUBSTANCE: what is offered is a molecular genetic diagnostic technique for nonsyndromic deafness (NSD) that implies detecting 17 disease-related GJB2 and GJB6 gene mutations by PCR amplification of appropriate regions conducted in 8 reaction mixtures with the use of specific primer pairs that is followed by analysing the produced amplicons either without preliminary endonuclease cleavage (in identifying the mutations c.312dell4, c.333-334delAA, ΔGJB6-D13S1830, ΔGJB6-D13S1854), or following the hydrolysis with appropriate specific restrictases (in identifying the mutations IVS1+1G>A, c.35delG, c.71G>A, c.79G>A, c.167delT, c.235delC, c.224G>A, c.299-300delAT, c.360delGAG, c.341A>G, c.269T>C, c.101T>C, c.109G>A).

EFFECT: use of the invention enables more accurate, objective diagnosis of inherited autosomal-recessive hearing loss.

2 tbl, 8 dwg, 3 ex

FIELD: medicine.

SUBSTANCE: mice are infected by the oral introduction of a suspension 0.1 ml of a two-day swine Rhodococcus equi, pathogenic of culture containing 5×107 CFU in normal saline 1.0 ml. The infection is followed the oral five-fold introduction of a probiotic preparation 1-5×105-6 CFU in 1.0 ml every 24 hours; in 5 days after the infection, the mice are killed, and dense egg medium are inoculated of the internals suspensions. In 1-2 cultivation days at optimal temperature, but not later than in 5 days, the grown colonies are controlled by acid fast stain. Antagonist activity is evaluated by a growth block index that is a relation of Rhodococcus colony count grown at inoculation of the internals suspensions of the animals prescribed with no probiotic preparations to Rhodococcus colony count grown at inoculation of the internals suspensions of the animals prescribed with the probiotic preparations. The efficacy of the probiotic preparation is concluded if the value exceeds 3.

EFFECT: invention provides fast and nocardioform actinomycete adequate screening of the probiotic preparations in vivo.

15 tbl, 14 ex

FIELD: medicine.

SUBSTANCE: microbial cultures are grown on LB-broth that is followed by McFarland standartisation to 0.5. The grown microbial cultures are introduced in wells of a polystyrene plate and added with LB-broth to form a monofilm or added with a broth culture to form a mixed biofilm. The plates are kept in a thermostat at 37°C for 48 hours to form biofilms. The formed biofilms are washed with distilled water and coloured with 0.1% aqueous gentian violet 200 mcl for 45 minutes in the dark, and the biofilms are thoroughly washed for three times with distilled water that is followed by drying the plate and colour intensity biofilm testing. For this purpose, the colourant is eluted in 96% alcohol; the eluate is analysed for an optical density in a spectrophotometer; this value matches to a film formation level; the average optical densities of the eluate of the mixed biofilms and the total optical densities of the eluate of the monoculture biofilms are compared, and if observing no reliable differences, the neutral pattern of interactions are stated; if the value for the mixed biofilm is less than the total value for the monofilms, the antagonistic pattern of interactions is concluded, while the value for the mixed biofilm exceeding the total values for the monofilms enables stating a synergistic pattern of the intermicrobial interaction. The total optical densities of the eluate of the monoculture biofilms shall not exceed 4.

EFFECT: invention enables evaluating the pattern of intermicrobial interaction and predicting a developing mixed infection.

1 tbl, 3 ex

FIELD: medicine.

SUBSTANCE: early diagnostic technique for sexually-transmitted infections involves sampling from a cow's cervical canal in 50-60 postpartum days within the preparation for insemination; inoculation of meat infusion agar with added 5% sheep erythrocytes, of yolk-saline agar, Endo medium, Ploskirev's medium, Enterococcus agar, Saburaud medium, and Zeisler agar with the latter to be incubated in an anaerobic culture apparatus. It is combined with inoculation of liquid high-column Blikfeldt medium and Bifidus medium, sugar broth. The inoculations are grown at temperature 37°C within a period optimal for each microorganism to be detected. The smears are Gram stained to identify the detected cultures by conventional techniques.

EFFECT: technique enables early diagnosis of the sexually-transmitted infections caused by opportunistic microflora, prevention of aborts, reproductive disorders, and well-timed prevention and treatment.

2 tbl

FIELD: chemistry.

SUBSTANCE: adhesive activity index (A), invasive activity index (I) and toxic activity index (T) of pathogenic bacteria is determined on continuous cells of a tissue culture of buffalo green monkey (BGM) kidney cells. A dose containing 107 colonies of bacterial cells is added to the culture monolayer of BGM cells and held at 37°C for 24 hours. The monolayer cells are washed 8 times with 10 ml of a physiological solution at pH 7.2. Index A is determined using a formula after first visually counting in Gorjaev's chamber living and dead cells stuck with bacterial growth and coloured by 0.1% trypan blue solution. Index (I) is determined using a formula after first replacing the culture medium in vials with a medium containing an antibiotic with concentration 50 mcg/ml and incubated in a temperature-controlled cabinet for 1 hour at 37°C. BGM cells are washed from bacteria 8 times with 10 ml of a physiological solution and lysed with 0.1% Triton X-100. Seeding of 0.1 ml is made from the obtained suspension based on the required cultivations per cup with Endo medium. Incubation is carried for 24 hours in a temperature-controlled cabinet at 37°C and the number of grown bacterial cells is counted. Index (T) is determined using a formula after first incubating bacteria in the BGM cell monolayer for 1 day. The suspension is centrifuged and filtered through membrane filters with pore diameter 0.22 mcm. The obtained substrate is put into a pure monolayer of continuous cells and the number of living and dead BGM cells is counted after one day.

EFFECT: invention enables to determine the degree of epidemic hazard of pathogenic bacteria isolated from water.

3 tbl, 4 ex

FIELD: medicine.

SUBSTANCE: potentially affected tissue and adjoining histologically normal tissue are examined for a level of RHOV gene transcription. A higher level of RHOV gene transcription in potentially affected tissue in comparison with the same in adjoining histologically normal tissue serves as a diagnostic sign of non-small-cell lung cancer. A polymerase chain reaction for the purpose of RHOV gene transcription is conducted by using a set of primers having sequences SEQ ID NO: 1 and 2.

EFFECT: invention provides high-reliable diagnosis of non-small-cell lung cancer, including squamous cell carcinoma and adenocarcinoma, including at the early stage of progressing cancer transformation.

12 cl, 1 tbl, 7 ex, 2 dwg

FIELD: medicine, psychiatry.

SUBSTANCE: one should isolate DNA out of lymphocytes of peripheral venous blood, then due to the method of polymerase chain reaction of DNA synthesis one should amplify the fragments of hSERT locus of serotonin carrier gene and at detecting genotype 12/10 one should predict the risk for the development of hallucino-delirious forms of psychoses of cerebro-atherosclerotic genesis.

EFFECT: more objective prediction of disease development.

3 ex

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