Method for production of lactobacillus delbrueckii subsp bulgaricus strain with low lactic acid postproduction degree, texturising strains produced (versions), dairy product and method of its production using texturising strains

FIELD: food industry.

SUBSTANCE: invention deal with a method for production of Lactobacillus delbrueckii subsp. bulgaricus strain with reduced lactic acid postproduction. The method involves exposure of Lactobacillus delbrueckii subsp. bulgaricus, mother strain possessing high texturising properties and high acid postproduction to the impact of a weak mutagen represented by ethyl methanesulfonate (EMS) and subsequent selection of strains with preset characteristics. Characterisation of selected Lactobacillus delbrueckii subsp. bulgaricus texturising strains (versions) is as follows: a) they have pH (measured by standard method) within the range of 4.25-4.55 after 14 days of storage; b) they have texturising properties (measured using viscosity determination analysis) no less than 25 Pa or a) hey reduce pH by at least 0.20 or 0.30 pH units after 14 days of storage at a temperature of 8°C; b) they have texturising properties (measured using viscosity determination analysis) no less than 25 Pa. Invention relates to application of the selected strains for production of fermented dairy products and dairy products containing Lactobacillus delbrueckii subsp. bulgaricus strain with the above characteristics.

EFFECT: invention enables production of a dairy product having more expressed texturising properties with less acid postproduction.

12 cl, 5 dwg, 2 ex

 

The technical FIELD TO WHICH the INVENTION RELATES

The present invention relates to lactic acid bacteria with a low or reduced ability to postproduction acid, and the method of production of such bacteria. Also the present invention relates to the use of such bacteria to obtain fermented milk products and dairy products containing bacteria.

The prior art INVENTIONS

Strains of the speciesLactobacillus delbrueckiisubsp.bulgaricusare essential components of bacterial cultures used, in particular, to obtain fermented milk and yoghurt. Typically, these strains produce lactic acid during storage of dairy products, fermented bacterial cultures, components which are those strains. This phenomenon is often referred to as "postproduction acid". Typically, these strains do not improve the texture of dairy products obtained by bacterial fermentation cultures, a component of which are those strains.

According to the legislation of most EU countries, the starter culture used for yogurt production, should include strains of typeLactobacillus bulgaricusand strains of typeStreptococcus thermophilus. In addition, the fermentation of milk is faster when the starter includes both the type strain is compared using the same type of strain.

Market trend fermented milk demonstrates the demand for products with reduced acid production or lack of production of acids during storage (low postproduction acid) with good texturise ability (or the giving of viscosity). When selecting strains of typeLactobacillus delbrueckiisubsp.bulgaricusseparately or as components of culture to obtain a fermented milk or yogurt product development teams are selected from known strains possessing the following combination of properties:

low postproduction acid and weak texturemode property; or

- high postproduction acid and weak texturemode properties;

- high postproduction acid and high texturemode properties; or from strains that are not strains ofLactobacillus delbrueckiisubsp.bulgaricusbut is available for a satisfactory combination of low profile postproductive acid with high textureloader profile.

When using any of the above known strains fermented dairy product will have a result or a high degree of postproductive acid, or weak texturemode properties as a result of the use of bacterial culture. Manufacturers of dairy products often choose to work cool the tours, strains whichLactobacillus delbrueckiisubsp.bulgaricuscombine low postproduction acid and weak texturemode properties. To improve the texture of fermented dairy products enter the thickening agent(s) into lactic basis before fermentation.

BRIEF description of the INVENTION

The problem before a specialist in the field of technology to which the present invention is to provide an alternative strains ofLactobacillus delbrueckiisubsp.bulgaricus,the combination of which has the combination of low profile postproductive acid and high textureloader profile, thus eliminating the need of adding thickening agents. Some scientists have tried to obtain such strains, but to no avail. In Mölleret al. (2001) describes chemical mutagenesis (mutagen MNNG (N-methyl-N-nitroso-N-nitroguanidine)) strainLactobacillus delbrueckiisubsp.bulgaricus(strain Visby 231) with the aim of obtaining mutants with reduced postproduction acid. The mutant strain were sown manually in a Petri dish, and colonies are manually transferred to a Cup of microtitration containing milk. After incubation, each well was measured pH to approximately 2000 isolates using microelectrode. Then identified four mutant with high ultimate pH in comparison with the parent strain. Described mutagenesis and crying has not resulted in mutants with low postproduction acid, allows to obtain a dense texture.

The authors of the present invention unexpectedly discovered that it is possible to obtain such strains and to develop a method of obtaining such strains, this method differs from the method described in Mölleret al. (2001), in which the authors present invention is used as parent strains strains, allowing you to get a good texture when using a weak mutagen. Carrying out the method according to the present invention, the present invention unexpectedly have identified strains with improved textureloader properties compared to the parent strains. Therefore, the authors of the present invention have developed a method of producing strains ofLactobacillus delbrueckiisubsp.bulgaricusthat essentially contribute to the improvement of the texture of dairy products obtained by fermentation of a strain of the present invention, or bacterial cultures, a component of which is the strain of the present invention, essentially without postproduction acid in dairy products.

The resulting dairy products have an attractive, softer, less acidic taste in comparison with the known products.

A DETAILED DESCRIPTION of the INVENTION

In the first aspect of the present invention relates to a strain ofLactobacillus delbrueckiisubsp.bulgaricus,that:

a) producer is no less acid, when present in fermented dairy product (e.g., during storage), compared with other strains of the same species, allowing to obtain a good texture; and/or

b) improves the texture of fermented milk product with a low postproduction acid compared with strains of the same species with a higher level of postproductive acid.

A variant of the embodiment of the present invention is a strain that is:

a) has a pH, measured in the standard way (tests postproductive acid), in the range of 4.25-4,55 (such as within 4,30-4,55; or in the range of 4.35-4,55) after 14 days and/or 21 and/or after 28 days of storage; and

b) has texturemode properties, measured in PA when using the analysis to determine the viscosity of not less than 25 (such as not less than 30; not less than 35; not less than 39; not less than 42; not less than 44; not less than 45; not less than 46; not less than 47; not less than 48 or not less than 50);

or strain that

a) reduces the pH, measured in the standard way (tests postproductive acid), less than 0,30 pH units (such as less than 0,20 pH units) after 14 days of storage; and

b) has texturemode properties, measured in PA when using the analysis to determine the viscosity of not less than 25 (such as not less than 30; not less than 35; the e less than 39; not less than 42; not less than 44; not less than 45; not less than 46; not less than 47; not less than 48 or not less than 50);

or strain that

a) reduces the pH, measured in the standard way (tests postproductive acid), less than 0,20 pH units (such as less than 0.15, less than 0,11; less than 0,08; less than 0,07; less than 0,06; less than 0.05, less than 0,04; less than 0.03 or even less than 0.02 pH units) after 7 days of storage at 8°C; and

b) has texturemode properties, measured in PA when using the analysis to determine the viscosity of not less than 25 (such as not less than 30; not less than 35; not less than 39; not less than 42; not less than 44; not less than 45; not less than 46; not less than 47; not less than 48 or not less than 50).

The term "reducing pH" shall mean the reduction in pH relative to the starting point, i.e. pH 4,55. Reduction, for example, 0.30 pH units means that the resulting pH is 4,25.

In another variant embodiment of the present invention decrease the production of acid is not because of substantial or complete inactivation of beta-galactosidase activity; and/or because of substantial or complete inactivation of the lactate-dehydrogenase activity of aktivnosti. The term "not because of the significant inactivation" refers to the decreased activity of less than 30% (such as less than n is 20% or even less than 10%).

Currently preferred is a strain ofLactobacillus delbrueckiisubsp.bulgaricus,which vibiraut from the group consisting of: CHCC10019; CHCC8535; and CHCC7159; and mutants of any of them, such as the functional equivalents of mutants, for example, mutants, which essentially have the same postproduction acid and texturemode properties, and the most preferred is the strain CHCC7159 or its mutants, such as the functional equivalents of mutants, for example, mutants, which essentially have the same postproduction acid and texturemode properties.

In the context of the present invention, the term "his mutant" should be understood as the strain of the present invention, obtained, for example, genetic engineering, treatment with radiation and/or chemical treatment. Preferably, the mutant was a functional equivalent mutant, for example the mutant, which essentially has the same properties of postproductive acid and texturise properties as the parent strain. This mutant is a part of the present invention. In particular, the term "his mutant" refers to a strain obtained by the effect on strain of the present inventionLactobacillus delbrueckiisubsp.bulgaricus(such as CHCC10019; CHCC8535; CHCC7159; CHCC3984; CHCC3606 or CHCC5713) any suitable used mutagenic treatment, on the tea processing chemical mutagen, such as ethane sulfonate, methane (EMS) or N-methyl-N'-nitro-N-nitroguanidine (NTG), UV light for spontaneous occurrence of mutations. Strain mutant preferably should perform at least one of a) and b) above combinations. Although currently it is preferable to obtain a strain of random mutation or selection by chance obtained mutants, i.e. without the use of recombinant DNA technology, the possibility of obtaining mutantsLactobacillus delbrueckiisubsp.bulgaricuswhen using such technology, including site directed mutagenesis and PCR technology and other modifications of DNA sequences in vitro or in vivo. The term "essentially the same postproduction acid", preferably it should be understood that the pH can vary by +/- 0.1 pH units (for example, 0.05; 0.01 or 0,00 units) relative to the parent strain, and the term "essentially the same textureloader properties", preferably it should be understood that the indicator PA can vary up to 20% (for example, up to 10% or up to 5%) and/or +10/-2 units (such as+5, +3, +/-2, +/-1,0; +/-0,5 or 0.0 units) relative to the parent strain.

In a second aspect the present invention relates to a method for producing strain ofLactobacillus delbrueckiisubsp.bulgaricusproducing less acid in fermented say cnom product (for example, during storage of the finished product) in comparison with the parent strain, the method includes processing strain ofLactobacillus delbrueckiisubsp.bulgaricus(parent strain) mutagen (preferably soft or weak mutagen), such as a mutagen selected from the group consisting of sulfonate ethyl methane (e.g., 1%); nitrous acid (for example, 0.05 M); sulfonate methyl methane (for example, 20 mm); nitrosoguanidine (for example, 25 μm); and acridine-mustard ICR-170 (e.g., 5 µg/ml), or with radiation, such as x-rays (for example, 2000 roentgen per minute); or UV rays (e.g., 600 erg/mm2per minute).

Currently preferred is a mutagen EMS (sulfonate ethyl methane). Currently preferred is that of the parent strain was the strain ofLactobacillus delbrueckiisubsp.bulgaricusthat has a good textureloader properties during growth in milk, but also high postproduction acid, such a strain selected from strains with the following properties:

a) strong extranervosae property, such as strain whose viscosity when determining, using the analysis is PA not less than 25 (such as not less than 30; not less than 35; not less than 39; not less than 42; not less than 44; not less than 45; not less than 46; not less than 47; not less than 48 or not less than 50); and

b) the strong property postproductive acid, such as strain, which reduces the pH, measured in the standard way (tests to determine the properties of postproductive acid), more than 0,20 pH units (such as or exceed 0.30 to 0.40, or even more than 0,50) after 7 days of storage.

Examples of such strains are CHCC3984, CHCC3606 and CHCC5713, and mutants of any of them (such as the functional equivalents of mutants, for example, mutants, which essentially have the same properties postproductive acid and texturing). These strains, along with strains ofLactobacillus delbrueckiisubsp.bulgaricus,obtained by the method according to the present invention, are all a part of the present invention.

In one variant embodiment of the present invention the method according to the present invention further includes:

- incubation of individual mutants in the environment (such as pH indicator cups; cups of microtitration containing environment, for example, with the addition of a pH indicator; or in test tubes containing environment, for example, with the addition of a pH indicator); and

- breeding mutant with significantly higher final pH of the medium after incubation in comparison with the parent strain.

It is convenient to measure the pH using a pH electrode, or by using a pH indicator such as Bromphenol purple/Bromphenol green, where the environment is milk, for example, the standard environment.

In additional the second aspect of the present invention relates to the use of strains of Lactobacillus delbrueckiisubsp.bulgaricusaccording to the present invention to obtain a fermented milk product such as yogurt.

Also the present invention relates to a method for producing a dairy product comprising mixing the strain of the present invention with milk, milk product, obtained in this way. The resulting dairy products are nicely soft and less sour taste in comparison with the previously known products.

In another variant embodiment of the present invention relates to a method for bacteriaLactobacillus delbrueckiisubsp.bulgaricusthat includes mixing the strain of the present invention, or compositions comprising the specified strain with growth medium, such as milk (e.g., standard environment).

In this latter aspect the present invention relates to compositions used for the fermentation of milk, including the strain ofLactobacillus delbrueckiisubsp.bulgaricusaccording to the present invention. An example of such composition is the leaven that contains the strain of the present invention along with additional strains or preservative additives. The composition may be dried, subjected to lyophilization, frozen or be in liquid form.

DEFINITIONS

In the context of the present invention, the term "milk which includes skim milk, milk, low-fat, full-fat milk, milk, free from lactose (obtained by hydrolysis of lactose by the enzyme lactase to glucose and galactose, or otherwise), concentrated milk or milk powder. Skim milk is milk from which removed the fat. Milk with low fat content is a milk with a fat content from about 1% to about 2%. Full fat milk is a milk with a fat content of about 2% or more. Used herein, the term "milk" also included in the scope of the milk obtained from an animal or vegetable source. The milk from an animal source includes, without limitation female, cow, sheep, goat, Buffalo, camel, llama, horse, and reindeer. Milk from vegetable source includes, without limitation, the milk extracted from soy beans or oats. In addition, the term "milk" refers not only to whole milk, but also to skim milk or any liquid component derived from it.

The term "strain with high textureloader properties", you should understand the strain that during inoculation of the medium at a ratio of 1×105- 1×106CFU/g at the incubation temperature of 43°C at pH 4,55 is the shear stress fermented milk exceeding 25 PA, the estimation using the app is RA 20 hours after the fermentation/coagulation, as described in "Analysis by determination of viscosity". In contrast, the term "strain with low textureloader properties", you should understand the strain that during inoculation of the medium at a ratio of 1×105- 1×106CFU/g at the incubation temperature of 43°C at pH 4,55 is the shear stress fermented milk less than 25 PA, the assessment with the use of device 20 hours after the fermentation/coagulation as described in the method of determining viscosity.

The term "strain with high postproduction acid" should be understood strain, when grown in accordance with the Tests for determining postproductive acid reduces the pH more than at 0.20 pH units after 7 days of storage (pH reduced from 4,55 to 4.35 or below). In contrast, the term "strain with low postproduction acid" should be understood strain, when grown in accordance with the Tests for determining postproductive acid reduces the pH of not more than 0,20 units pH after 7 days of storage (pH reduced from 4,55 to 4.35 or the pH is in the range from 4,55 to 4.35, including both endpoints).

The term "standard environment" should be understood reconstituted milk made from skim milk powder with a solids content of 9.5%, since heat treatment at 99°C for 15 minutes with periodic fashion production.

The terminology used here is provided only as a description of the specific variants of the embodiment of the present invention and does not restrict the scope of claims of the present invention (unless explicitly specified otherwise). Used here and in the appended claims, the singular number include the plural, unless the context clearly is not visible otherwise. The words "include," "includes" and "including" should be interpreted in the open and not a closed value. All these indicators in the form of intervals include each individual indicator, included in this interval, unless explicitly stated otherwise. All methods described here can be implemented in a suitable manner, if this is not specified, or if they clearly are not inconsistent with the context. All the examples or any example or introductory words used to specify (for example, "such as"), is used only to facilitate understanding of the present invention and they do not limit the scope of its claims set forth in the claims.

DESCRIPTION of FIGURES

Figure 1 represents a curve of the dependence of pH with time at a temperature of 43°C for strain-mutant CHCC8535 and maternal strain CHCC5713. Example 1.

Figure 2 represents a curve of the dependence of pH with time at a temperature of 43°C for strain-mutant CHCC7159 and mate the warrior strain CHCC3606. Example 1.

Figure 3 represents the average shear stress measured on three independent samples, obtained using different strains of typeLactobacillus delbrueckiisubsp.bulgahcus. Interval error values averaged about 2 standard deviations from the mean.

Figure 4 represents a curve of the dependence of pH with time at a temperature of 43°C strain of mutant CHCC10019 and maternal strain CHCC3984. Example 2.

Figure 5 represents the average shear stress measured on three independent samples, obtained using a strain of mutant CHCC10019 and maternal strain CHCC3984. Interval error values averaged about 2 standard deviations from the mean. Example 2.

EXAMPLES

The main methods

According to the method of the present invention the parent strain ofLactobacillus delbrueckiisubsp.bulgaricushigh textureloader properties and high postproduction acid is treated with a chemical mutagen. Among the resulting strains conduct screening of mutants with low postproduction acid and mutants with high textureloader properties. Below method is described in detail.

Analysis to determine the viscosity (texturebrush properties)

For inoculation of 200 ml of milk recovered from skimmed milk powder (standartised), used frozen concentrates strains. The milk solids content of 9.5% was subjected to treatment at a temperature of 99°C for 15 minutes under atmospheric pressure at periodic method of obtaining. Frozen concentratesLactobacillus bulgaricus,as a rule, include from 1×109up to 1×1010CFU/g Figure inoculation is 1 g of concentrate per 10 litres of milk, therefore, from 1×105up to 1×106CFU/ml of milk. Incubation is carried out at a temperature of 43°C to achieve a pH 4,55, when milk coagulates. Then the fermented milk is cooled to a temperature of 5°C with periodic mixing (by placing in an ice bath for 20 minutes, followed by a space in the refrigeration device).

After 20 hours after reaching pH 4,55 fermented milk is heated up to 13°C and gently mixed using a rod, provided with a perforated disk (diameter=3 cm)to achieve homogeneity of the sample. Mixing consists of 20 movements of the rod up and down in the sample. The rheological properties of the sample were evaluated at a temperature of 13°C in the rheometer (StressTech, Reologica Instruments, Sweden), equipped with coaxial measurement system with load/Cup (CC25).

Test the viscometer were indicators of the shearing forces in the range from 0.27 to 300 1/s in stage 21. Indicators of shearing forces increased and C is the reduced, built curve of shear stress and recorded a clear increase and decrease of viscosity. Time delay integration and balance amounted to 5 seconds, 10 seconds and 5 minutes, respectively. For additional analysis chose the shear stress, equal to 300 1/s

The shear stress was measured in PA. To determine the viscosity is equal to the shear stress divided by the rate of shear. Therefore, the viscosity and the shear stress is directly proportional to and Express the same properties that correspond to the indicators of shear stress defined at a fixed rate of shear 300 1/s

Analysis determine postproductive acid

For inoculation of 200 ml of milk recovered from skimmed milk powder (standard environment), used frozen concentrates strains. The milk solids content of 9.5% was subjected to treatment at a temperature of 99°C for 15 minutes with occasional method.

Frozen concentratesLactobacillus bulgaricus,as a rule, include from 1×109up to 1×1010CFU/g Figure inoculation is 1 g of concentrate per 10 litres of milk, therefore, from 1×105up to 3×106CFU/ml of milk. Incubation is carried out at a temperature of 43°C to achieve a pH 4,55, when milk coagulates. Then the fermented milk is cooled to a temperature of 5°C with periodic mixing, placing in an ice bath for 20 minutes, followed by a space in the refrigeration device). the pH of the cooled fermented milk was measured using a pH electrode, respectively, after 1; 7; 14; 21 and 28 days of storage.

The method of obtaining a standard environment

Dissolve skimmed milk powder (ArIa Ingredients, Denmark - Milex 240 medium heat milk powder) in water for 30 minutes. Get a milk solids content of 9.5% (weight/weight).

Milk should be sterilized at the following temperature conditions:

Temperature(°C)Time (min)
Heating->99±1<20
Sterilization99±115±1
Cooling99±1->40±5<40

The milk is then stored at a temperature of <7°C. the Milk should not be used the next day.

Reproduction of bacteria.

Well-known strains of the speciesLactobacillus delbrueckiisubsp.bulgaricus,to improve the texture of fermented milk and postproduction acid. Such strains are preferably used as parent strains. Bacterial strains, producer the matter of lactic acid, add in a slurry form, liofilizirovannogo powder or frozen granules in milk for fermentation. The number of bacteria in liquid, powder and granules can range from 1E6to 1E12colonies forming units per gram (1×106up to 1×1012CFU/g)using the standard method of counting cells. Liquid, powder or granules, as a rule, add in the milk in an amount of from about 0.0005 to about 1%.

Mutagenesis.

Bacteria, such as strains ofLactobacillus delbrueckiisubsp.bulgaricusthat allow you to get a firm texture when growing in milk, but also possess a high postproduction acid, is subjected to chemical mutagenesis, preferably a weak mutagen, such as EMS (sulfonate ethyl methane). EMS gives rise to random substitution of base pairs in the genetic material (chromosomes and plasmids) mutated bacteria. After mutagenesis, the mutated culture plated on medium in Petri dishes.

The preferred Protocol procedure: parent strains inoculant from the stored at a low temperature of MRS broth (Difco) for 24 hours at 37°C, anaerobic. For mutagenesis overnight culture was stirred for 1 minute with the formation of a crater at maximum speed to separate the alleged chains of cells and treated by EMS (10 ml/ml) during the course is 2 hours at 37°C. As a result of processing EMS more than 99% of the cells died. EMS-treated culture was frozen at a temperature of -80°C in 20% glycerol and stored for subsequent screening.

Screening

Several thousand colonies randomly chosen using a robotic device that selects a colony in cups microtitration containing 200 μl of milk, for example, by adding a pH indicator (such as Bromphenol purple/Bromphenol green). Each hole in the Cup microtitration corresponds to the model fermentation of milk. the pH of milk in the wells containing the mutants, compared with the corresponding wild-type strain (strainLactobacillus delbrueckiisubsp.bulgaricusthat allows you to get a firm texture during growth in milk, but also has a high postproduction acid, such as strains ofLactobacillus delbrueckiisubsp.bulgaricusCHCC3984, CHCC3606 and CHCC5713). Identified mutants with significantly higher final pH of milk after fermentation at a temperature of 43°C compared with wild-type strains, for example, when using colorimetric evaluation (using pH indicator) or by using a pH electrode. The fermentation is carried out in large scale (200 ml), recording the pH in the system registering the device with a standard pH electrodes to confirm that the mutant cause less postproduction acid.

For control of the I, the resulting mutants have the same texturearray capacity as parent strains, samples of milk were fermentatively mutant and parent strains to the same final pH was evaluated and compared the rheological properties of the obtained fermented milk using standard technologies.

Example 1: preparation and properties of strains LB CHCC7159 and CHCC8535

CHCC7159 and CHCC8535 are bacteria that produce lactic acid, typeLactobacillus bulgaricus. These strains are able to skachivat milk in terms of industrial production and differ in that they have low postproduction acid. Strain CHCC7159 is a mutant derived from CHCC3606 with which it was compared. CHCC8535 is a mutant CHCC5713 with which it was compared. Mutagenesis (preferred Protocol of the procedure) and screening were performed as described above.

For inoculation of 200 ml of milk recovered from skimmed milk powder (standard environment), used frozen concentrates strains CHCC7159, CHCC8535, CHCC3606 and CHCC5713. The milk solids content of 9.5% was subjected to treatment at a temperature of 99°C for 15 minutes with occasional way. Frozen concentratesLactobacillus bulgaricus,as a rule, include from 1×109up to 3×1010CFU/g Indicator inoculation is the tsya 1 g of concentrate per 10 litres of milk, therefore, from 1×105up to 3×106CFU/ml of milk. Incubation was carried out at a temperature of 43°C to achieve a pH 4,55, when milk coagulates. Then the fermented milk was cooled to 5°C.

Curve of pH against time shows that CHCC8535 reaches pH 4,60 less than 20 hours, the resulting strains capable of skachivat milk under conditions of inoculation and incubation, which is industrially applicable (figure 1).

Curve of pH against time shows that CHCC7159 reaches a pH of 4.60 for about 21 hours, strain, capable of skachivat milk under conditions of inoculation and incubation, which is industrially applicable (figure 2).

CHCC7159 and CHCC8535 when fermentation has produced less acid than the corresponding parent strains. CHCC8535 has a pH of 4.17 after 20 hours; that is by 0.32 units higher compared with the parent strain. CHCC7159 has a pH with 4.64 after 20 hours; that is by 0.50 pH units higher compared with the parent strain.

CHCC7159 and CHCC8535 allow you to get an improved structure of fermented milk

The next day after incubation, the fermented milk was heated up to 13°C and gently mixed using a rod, provided with a perforated disk, to achieve homogeneity of the sample. The rheological properties of the sample on univali on the rheometer (StressTech, Reologica Instruments, Sweden), equipped with C25 coaxial measurement system. Test the viscometer were indicators of the shearing forces in the range from 0.27 to 300 1/s in stage 21. Indicators of shearing forces increased and then decreased, built curve of shear stress and recorded a clear increase and decrease of viscosity. Time delay integration and balance amounted to 5 seconds, 10 seconds and 5 minutes, respectively. For additional analysis chose the shear stress, equal to 300 1/s

Fermented milk, incubated with concentrates CHCC7159 and CHCC8535, had a more pronounced texture compared to the fermented milk obtained using the corresponding parent strains (Figure 3). For the two mutant strains average recorded indicators shear stress ranged from 45 to 55 PA, while for the parent strains indicators amounted to about 40 PA or less.

Conclusion

Strains CHCC7159 and CHCC8535 combine the following properties:

- allow you to get a solid texture of fermented milk in excess of 40 PA in the experimental testing conditions,

- able to acidify the milk to pH 4,60 less than 24 hours,

- reach a pH above 4,15 after 20 hours under test conditions.

Example 2: preparation and properties of strain LB CHCC10019

CHCC10019 is a milk acid bacteria type> Lactobacillus bulgaricus. CHCC10019 able to skachivat milk in an industrial environment and has a low postproduction acid. Strain CHCC10019 is a mutant obtained CHCC3984 with which it was compared. Mutagenesis (preferred Protocol of the procedure) and screening were performed as described above. Used the mutagen was an EMS.

During the night of culture CHCC10019 and CHCC3984 received by insulinopenia in 10 ml restored skimmed milk powder. The milk had a solids content of 9.5% and was heat treated at a temperature of 99°C for 15 minutes with occasional way. Biological material was obtained from frozen vials with the corresponding strains. Incubation was carried out at 37°C for 24 hours.

During the night of culture CHCC10019 and CHCC3984 used for inoculation of 200 ml restored skimmed milk powder (standard environment). Used milk represented the same as described above. The quantity of starter culture was 1%. Each strain were incubated in two bottles of milk. One bottle was used to obtain the curve of production of acid; the second bottle was used to obtain product for determining the rheological properties.

During the night the number of cellsLactobacillus bulgaricus,usually ranges from 1×106up to 1×108CFU/g P is the index of inoculation during the night amounted to 1 g of culture per 100 g of milk, therefore, ranged from 1×104up to 1×106CFU/ml of milk.

Incubation was carried out at a temperature of 43°C for 20 minutes. The second bottle was treated under the same conditions, were incubated at a temperature of 43°C to achieve a pH 4,55; when the milk coagulates. This bottle was cooled to 5°C before analysis on the flow properties the next day.

Curve of pH against time shows that CHCC10019 reaches pH 4,50 for about 7 hours and 30 minutes, the resulting strain can skachivat milk in conditions of inoculation and incubation, which is industrially applicable, see figure 4.

CHCC10019 when fermentation has produced a smaller amount of acid compared to the corresponding parent strain. CHCC10019 has a pH after 20 hours 4,22; which by 0.64 pH unit higher than the parent strain.

CHCC10019 allows you to get an improved structure of fermented milk.

The next day after incubation, the fermented milk was heated up to 13°C and gently mixed using a rod, provided with a perforated disk to achieve homogeneity of the sample. The rheological properties of the sample was evaluated as in Example 1. The results, see figure 5. Fermented milk, incubated with CHCC10019, had a more pronounced texture compared to CHCC3984. For the mutant strain is the average of the recorded indicators shear stress was 45 PA, while for the parent strain indicators amounted to about 35 PA.

CHCC7159 deposited on 8 February 2006 in the German collection of microorganisms and cell cultures GmbH (DSM) and assigned inventory number DSM 17959. CHCC3606, CHCC5713 and CHCC8535 deposited on 30 March 2006 in the DSM and assigned the accession numbers DSM 18142, DSM 18143, and DSM 18144, respectively. CHCC10019 and CHCC3984 deposited on 3 April 2007 by assigning accession numbers DSM 19252 and DSM 19251, respectively. The deposition was carried out in accordance with the Budapest Treaty on the international recognition of the Deposit of microorganisms for patent.

Preferred variants of the embodiment of the present invention, described here, are the best ways to embodiments of the present invention. Specialist in the field of engineering that applies the present invention, it should be understood that the preferred variants of the embodiment of the present invention can have sub-options. The present invention is not limited to the variants described here and can be implemented in other ways than described here understood by the expert from the prior art. Therefore, the present invention includes all modifications and equivalents of the objects of the present invention, as claimed in the attached claims, in accordance with the law. the moreover, all combinations of the described elements in all possible variations are included in the scope of the present invention, if they are in the context of the present invention and does not explicitly contradict him.

LINKS

Möller, C., Bockelmann, W., and K. J. Heller,2001. Isolierung vonLactobacillus delbrueckiisubsp.bulgaricusmutanten zur herstellung a joghurts mit milder geschmackscharakteristik. Kieler Milchwirtchaftliche forchungsberichte 53, 167-185.

WO 2006/063142

EP518096A1

EP638642A

US5545554A

Guide To Short-Term Tests For Detecting Mutagenic And Carcinogenic Chemicals. Prepared for the IPCS by the International Commission for Protection Against Environmental Mutagens and Carcinogens. WHO, 1985. http://www.inchem.org/documents/ehc/ehc/ehc51.htm

All references cited in this patent document, entered in full.

0-1From PCT/RO/134 (SAFE) Indications concerning deposited microorganism(s)
or other biological material (PCT Rule 13bis)
Published online version RST.
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0-1-1Ready to use
0-2No. of international publications
0-3Registration number of the applicant or patent attorneyP2253PC00

1The instructions below relate to the deposited microorganism(s) or other biological material with a link

1-1on description:
paragraph number
page 13, line 7
1-3Identification of Deposit
1-3-1Name of DepositoryDSMZ DSMZ - German collection of microorganisms and cell cultures GmbH
1-3-2Address of DepositaryMascheroder Weg 1b, D-38124 Braunschweig, Germany
1-3-3Date Deposit8 February 2006 (08.02.2006)
1-3-4Inventory numberDSMZ 17959
1-5State which made indicationsall of these
2Given neither the e instructions relate to the deposited microorganism(s) or other biological material with reference to the description:
2-1paragraph numberpage 13, line 9
2-3Identification of Deposit
2-3-1Name of DepositoryDSMZ DSMZ - German collection of microorganisms and cell cultures GmbH
2-3-2Address of DepositaryMascheroder Weg 1b, D-38124 Braunschweig, Germany
2-3-3Date Deposit30 March 2006 (30.03.2006)
2-3-4Inventory numberDSMZ 18142
2-5State which made indicationsall of these
3The instructions below relate to the deposited microorganism(s) or other biological material with reference to the description:
3-1paragraph numberpage 13, line 9
3-3Identification of Deposit
3-3-1Name of DepositoryDSMZ DSMZ - German collection of microorganisms and cell cultures GmbH
3-3-2Address of DepositaryMascheroder Weg 1b, D-38124 Braunschweig, Germany
3-3-3Date Deposit30 March 2007 (30.03.2007)
3-3-4Inventory numberDSMZ 18143
3-5State which made indicationsall of these

4The instructions below relate to the deposited microorganism(s) or other biological material with reference to the description:
4-1paragraph numberpage 13, line 9
4-3Identification of Deposit
4-3-1 Name of DepositoryDSMZ DSMZ - German collection of microorganisms and cell cultures GmbH
4-3-2Address of DepositaryMascheroder Weg 1b, D-38124 Braunschweig, Germany
4-3-3Date Deposit30 March 2007 (30.03.2007)
4-3-4Inventory numberDSMZ 18144
4-5State which made indicationsall of these
5The instructions below relate to the deposited microorganism(s) or other biological material with reference to the description:
5-1paragraph numberpage 13, line 9
5-3Identification of Deposit
5-3-1Name of DepositoryDSMZ DSMZ - German collection of microorganisms and cell cultures GmbH
5-3-2Address of DepositaryMascheroder Weg 1b, D-38124 Braunschweig, Germany
5-3-3Date Deposit3 April 2007 (03.04.2007)
5-3-4Inventory numberDSMZ 19252
5-5State which made indicationsall of these
6The instructions below relate to the deposited microorganism(s) or other biological material with reference to the description:
6-1paragraph numberpage 13, line 10
6-3Identification of Deposit
6-3-1Name of DepositoryDSMZ DSMZ - German collection of microorganisms and cell cultures GmbH
6-3-2Address of DepositaryMascheroder Weg 1b, D-38124 Braunschweig, Germany

6-3-3Date Deposit3 April 2007 (03.04.2007)
6-3-4Inventory numberDSMZ 19251
6-5State which made indicationsall of these

Only for a receiving office

0-4This form was obtained for international publication (Yes or no)
0-4-1Authorized person

Only for international Bureau

0-5This form was received by the International Bureau
0-5-1Authorized person

1. The method of obtaining Lactobacillus delbrueckii subsp. bulgaricus, which produces less acid, when present in fermented dairy product, compared with the parent strain, where the method includes processing of Lactobacillus delbrueckii subsp. bulgaricus weak mutagen, DG is the parent strain is selected from strains with the following properties:
a) high texturemode properties and
b) high postproduction acid.

2. The method according to claim 1, where the parent strain is selected from strains with the following properties:
a) high texturemode properties, that is, the strain has texturemode properties measured using the analysis to determine the viscosity of not less than 25 PA; and
b) high postproduction acid, i.e. strain, which lowers the pH more than at 0.20 pH units after 7 days of storage.

3. The method according to claim 1 or 2, where the resulting strain Lactobacillus delbrueckii subsp. bulgaricus, characterized by the following:
a) has a pH, measured in a standard way, in the range of 4.25-4,55 after 14 days of storage and
b) has texturemode properties measured using the analysis to determine the viscosity of not less than 25 PA.

4. The method according to claim 1 or 2, where the resulting strain Lactobacillus delbrueckii subsp. bulgaricus, characterized by the following:
a) lowers the pH, measured in a standard way, for less than 0,30 pH units after 14 days of storage and
b) has texturemode properties measured using the analysis to determine the viscosity of not less than 25 PA.

5. The method according to claim 1 or 2, where the resulting strain Lactobacillus delbrueckii subsp.bulgaricus, characterized by the following:
a) lowers the pH, measured in a standard way, for less than 0,20 units pH after 7 days of storage at 8°C; and
b) has texturemode properties is, measured using the analysis to determine the viscosity of not less than 25 PA.

6. Texturearray Lactobacillus delbrueckii subsp.bulgaricus CHCC 10019 (DSM 19252)obtained by the method according to any one of claims 1 to 5.

7. Texturearray Lactobacillus delbrueckii subsp.bulgaricus CHCC8535 (DSM 18144)obtained by the method according to any one of claims 1 to 5.

8. Texturearray Lactobacillus delbrueckii subsp.bulgaricus CHCC7159 (DSM 17959)obtained by the method according to any one of claims 1 to 5.

9. Dairy product obtained by the process comprising mixing strain on any of PP-8 with milk.

10. Dairy product obtained by the process comprising mixing Lactobacillus delbrueckii subsp.bulgaricus, which is obtained by the method according to any one of claims 1 to 5 with milk.

11. A method of obtaining a milk product, which comprises mixing strain on any of PP-8 with milk.

12. A method of obtaining a milk product, which comprises mixing Lactobacillus delbrueckii subsp.bulgaricus, which is obtained by the method according to any one of claims 1 to 5 with milk.



 

Same patents:

FIELD: chemistry.

SUBSTANCE: Saccharomyces cerevisiae yeast strain, which is isolated from fermented milk whey and can be cultured on dairy cheese whey, is deposited in the Russian National Collection of Industrial Microorganisms under number VKPM Y-3414 and is a producer of ethyl alcohol.

EFFECT: invention enables to obtain a microbial strain which is adapted to culturing on dairy cheese whey and solid media and increases output of ethyl alcohol.

1 tbl, 1 ex

FIELD: chemistry.

SUBSTANCE: disclosed is a Saccharomyces cerevisiae VKPM Y-3415 yeas strain which produces ethyl alcohol. The strain is adapted to culturing on dairy cheese whey.

EFFECT: strain is capable of producing ethyl alcohol with high output and is antagonistic to accompanying microflora.

1 ex

FIELD: agriculture.

SUBSTANCE: strain Streptomyces cellulosae WH9, deposited in CGMCC under the number NO.2167 and used to produce a microbial fertiliser. The strain Aspergillus versicolor WH13, deposited in CGMCC under the number NO.2171 and used to produce a microbial fertiliser. The microbial phosphate fertiliser contains a product of fermentation of a microbial composition containing the following four microorganisms: a strain Bacillus subtilis WH2 (CGMCC NO.0395.2), a strain Bacillus licheniformis WH4 (CGMCC NO.0395.4), a strain Streptomyces cellulosae WH9 and a strain Aspergillus versicolor WH13. Also the method is provided to manufacture the specified microbial phosphate fertiliser, where production of the specified microbial phosphate fertiliser may include using the ground phosphate rock containing 8%-12% P2O5.

EFFECT: improved properties of the strain.

8 cl, 3 tbl, 8 ex

FIELD: agriculture.

SUBSTANCE: strain Streptomyces cellulosae WH9, deposited in CGMCC under the number NO.2167 and used to produce a microbial fertiliser. The strain Aspergillus versicolor WH13, deposited in CGMCC under the number NO.2171 and used to produce a microbial fertiliser. The microbial phosphate fertiliser contains a product of fermentation of a microbial composition containing the following four microorganisms: a strain Bacillus subtilis WH2 (CGMCC NO.0395.2), a strain Bacillus licheniformis WH4 (CGMCC NO.0395.4), a strain Streptomyces cellulosae WH9 and a strain Aspergillus versicolor WH13. Also the method is provided to manufacture the specified microbial phosphate fertiliser, where production of the specified microbial phosphate fertiliser may include using the ground phosphate rock containing 8%-12% P2O5.

EFFECT: improved properties of the strain.

8 cl, 3 tbl, 8 ex

FIELD: agriculture.

SUBSTANCE: strain Streptomyces cellulosae WH9, deposited in CGMCC under the number NO.2167 and used to produce a microbial fertiliser. The strain Aspergillus versicolor WH13, deposited in CGMCC under the number NO.2171 and used to produce a microbial fertiliser. The microbial phosphate fertiliser contains a product of fermentation of a microbial composition containing the following four microorganisms: a strain Bacillus subtilis WH2 (CGMCC NO.0395.2), a strain Bacillus licheniformis WH4 (CGMCC NO.0395.4), a strain Streptomyces cellulosae WH9 and a strain Aspergillus versicolor WH13. Also the method is provided to manufacture the specified microbial phosphate fertiliser, where production of the specified microbial phosphate fertiliser may include using the ground phosphate rock containing 8%-12% P2O5.

EFFECT: improved properties of the strain.

8 cl, 3 tbl, 8 ex

FIELD: agriculture.

SUBSTANCE: strain Streptomyces cellulosae WH9, deposited in CGMCC under the number NO.2167 and used to produce a microbial fertiliser. The strain Aspergillus versicolor WH13, deposited in CGMCC under the number NO.2171 and used to produce a microbial fertiliser. The microbial phosphate fertiliser contains a product of fermentation of a microbial composition containing the following four microorganisms: a strain Bacillus subtilis WH2 (CGMCC NO.0395.2), a strain Bacillus licheniformis WH4 (CGMCC NO.0395.4), a strain Streptomyces cellulosae WH9 and a strain Aspergillus versicolor WH13. Also the method is provided to manufacture the specified microbial phosphate fertiliser, where production of the specified microbial phosphate fertiliser may include using the ground phosphate rock containing 8%-12% P2O5.

EFFECT: improved properties of the strain.

8 cl, 3 tbl, 8 ex

FIELD: medicine.

SUBSTANCE: recombinant method is used to produce a microorganism of Mycobacterium tuberculosis complex which involves phoP gene inactivation or deletion and fadD26 gene inactivation or deletion. The produced microorganism is used for preventing tuberculosis in humans and animals.

EFFECT: invention allows producing the vaccine microorganism showing the properties of high attenuation and immune protection against tuberculous infection.

7 cl, 27 dwg, 9 ex

FIELD: agriculture.

SUBSTANCE: invention represents reference strains Francisella tularensis of tularensis subtype, including two genetic groups AI and AII, of holartica subtype, including biovars japonica, erythromycin-sensitive and erythromycin-resistant, Erys and EryR, and of mediasiatica subtype. Strains are deposited in the State Collection of Pathogenic Microbes "Microbe". All strains are natural, apart from the strain of holartica subtype, biovar japonica, which is extracted from a human being. Strains are produced by selection of clones that stably preserve phenotypic properties and containing genes, nucleotide sequence of which is specified for the type and subtype. The set of reference DNA preparations is created on the basis of these strains and may be used for genetic and immunological research.

EFFECT: using the invention will make it possible to increase efficiency of diagnostic research, to standardise and to increase quality of diagnostic preparations at production stages.

4 cl, 4 dwg, 1 tbl, 10 ex

FIELD: biotechnology.

SUBSTANCE: invention method shall be implemented by inoculation of clinical material from incubation to the surface of a plate-like chromogenic medium Uriselect which is a non-selective agar medium rich in peptone and tryptophane, produced by Bio-Rad (France) by sectorial inoculation method. The culture plate shall be incubated in anaerobic and microaerophilic (5%-10% CO2) conditions, topt =+35+37ºC, for 48-72 hours. If required, repeated inoculation of the grown colonies of Leptotrichia isolates shall be performed, wherein the passage shall be performed by hatching and/or by a sterile cotton pad. The passages shall be incubated in the same conditions. The method for Leptotrichia cultivation using the Uriselect medium enhances the efficiency of Leptotrichia isolates from the clinical material and enables presumable identification of Leptotrichia by substrate color changes, cut strain loss in the event of repeated passages. The cultivation efficiency of the difficult-to-cultivate Leptotrichia - oral microbiocenosis residents in a human oral cavity is over 30%, the method being easy to implement and does not require costly equipment.

EFFECT: increased efficiency of Leptotrichia cultivation using the Uriselect medium, significant cost reduction.

3 cl, 1 tbl, 2 ex

FIELD: medicine, pharmaceutics.

SUBSTANCE: what is offered in application of at least one strain of probiotic bacteria specified in Lactobacillus plantarum 299, DSM 6595, Lactobacillus plantarum 299v, DSM 9843, Lactobacillus plantarum HEAL 9, DSM 15312, Lactobacillus plantarum HEAL 19, DSM 15313, Lactobacillus plantarum HEAL 99, DSM 15316, Lactobacillus paracasei 8700:2, DSM 13434 and Lactobacillus paracasei 02A, DSM13432 for preparing a composition for treating and/or preventing a viral infection caused by 'cold' virus, and a related method for treating and/or preventing. A number of days for which 'cold' symptoms are experienced has been also reduced in a group of patients having been taking a probiotic.

EFFECT: what is shown is intensified immune protection against antiviral infection that promotes decreasing 'cold' episodes in comparison with the controls.

16 cl, 13 dwg, 3 ex

FIELD: medicine.

SUBSTANCE: invention refers to applying a microorganism of Lactobacillus genus, characterised by ability to specific binding with bacterium of mutans Streptococci group in which specific binding is characterised by: (i) thermal stability; and/or (ii) proteolytic stability; and/or (iii) calcium dependence; and/or (iv) formation at pH within 4.5 to 8.5; and/or (v) formation with saliva added for preparing an anti-caries composition for treating or preventing caries caused by mutans Streptococci different from Streptococcus mutans. The specific binding is analysed by the following procedures: (a) cultivation of said microorganism to a steady state; (b) mixing of said microorganism and bacterium of mutans Streptococci group which is cultivated to the steady state; (c) incubation of the mixture prepared at the stage (b) in the environment allowing formation of the aggregated microorganism and bacterium of mutans Streptococci group; and (d) detection of the aggregates by the presence of the pellet. A method for preventing and treating caries caused by mutans Streptococci different from Streptococcus mutans involves introduction of the microorganism of Lactobacillus genus showing the ability to specific binding with bacterium of mutans Streptococci group in which specific binding is characterised by the properties specified above (i)-(v).

EFFECT: invention provides specific binding of said microorganism of Lactobacillus genus with such strains mutans Streptococci different from Streptococcus mutans which are cariogenous dental pathogens.

24 cl, 3 dwg, 1 tbl, 27 ex

FIELD: chemistry.

SUBSTANCE: invention relates to biochemistry, particularly protection of plants from diseases caused by phytopathogenic bacteria and phytoplasma. The disclosed agent is obtained by preparing a producer inoculum, growing the producer on a fermentative medium, drying the culture fluid, extracting a macrolide complex using lower alcohols, concentrating the extract and mixing said extract with additives and solvents in the following ratio, wt %: macrolide complex 17.0-23.5, solvents and additives - up to 100, where the producer used is a Streptomyces fradiae ARRIAM-53 strain.

EFFECT: invention increases plant protection effectiveness.

2 tbl, 10 ex

FIELD: medicine.

SUBSTANCE: invention concerns a method for standartisation of control seed strain of chicken cholera agents (Pasteurella multocida). The presented method involves cloning of the S- or M-form Pasteurella strain, gradual passaging by intramuscular, and then intranasal introduction in a susceptible organism of weight about 350 g; in 3 hours following after the death of every infected body, the microorganism is respectively isolated into physiological saline, and sealed by into 0.5-1.0 ml Pasteur pipettes with the Pasteurella strain isolated by the intramuscular passage being used over a period of 15-20 days as a by-product for the intranasal passage, while the Pasteurella strain isolated by the intranasal passage is used 2-3 days following the isolation procedure during the next 3 days as a reduction for producing a 10-hour (9 h at 37 and 1 h at 20°C) broth culture of encapsulated Pasteurellas.

EFFECT: invention enables producing the first-generation culture of encapsulated Pasteurellas recovered from the body with a specific microbial cell titre with these cells to be used for producing biopreparations.

15 dwg, 4 ex

FIELD: chemistry.

SUBSTANCE: dry enzymatic peptone, glucose, dialysate of baker's yeast and microbiological agar are mixed in given quantities. The obtained mixture is added to 1 litre of distilled water and boiled until complete dissolution of the agar. While hot, the mixture is poured into vials, sterilised in an autoclave and cooled to 47-55°C. The cooled medium is poured into Petri dishes and held until complete setting of the gel. Weighed zinc nanopowder in a physiological solution in amount of 0.005 mcg of zinc nanopowder per 0.1 ml of the physiological solution per Petri dish is deposited on the solid surface of the gel in aseptic conditions.

EFFECT: invention increases accuracy of results of investigating microbial contamination of air.

2 tbl, 1 ex

FIELD: veterinary science.

SUBSTANCE: invention relates to application of Bifidobacterium longum ATCC BAA-999 in production of a medicinal agent or a therapeutic nutrient composition for treatment of inflammatory bowel disease in a mammal and in production of a medicinal agent or a therapeutic nutrient composition for suppression of bowel inflammation in a mammal. The therapeutic nutrient composition is a nursery mix and fodder for pets and contains from 104 to 1012 CFU/g of Bifidobacterium longum ATCC BAA-999 to the weight of dry base.

EFFECT: invention provides for demonstration of high anti-inflammatory activity of the above probiotic strain of bifidus bacteria.

12 cl, 4 dwg, 3 tbl, 4 ex

FIELD: medicine.

SUBSTANCE: nutrient composition contains the nonpathogenic bacterial strain Lactococcus rhamnosus ATCC 53103 or Lactococcus rhamnosus CGMCC 1.3724 able to stimulate a systemic immune response, and the nonpathogenic bacterial strain Micrococcus varians MCV8 or Streptococcus salivarius DSM 13 084 able to have bacteriostatic and bactericidal effect on pathogens related to the onset of otitis, such as Streptococcus pneumoniae, non-typed Haemophilus influenzae and Moraxella catarrhalis The composition contains 104 to 1012 CFU/g of fresh weight of the composition of each strain. The composition additionally contains Streptococcus thermophilus and additionally contains at least one prebiotic in the amount of 0.3 to 6% of composition weight. The composition represents a baby formula or a food additive.

EFFECT: invention provides an effective protection against nasopharyngeal mucosa colonisation with pathogenic bacteria related with the onset of otitis.

7 cl, 1 tbl, 2 ex

FIELD: veterinary science.

SUBSTANCE: composition comprises a source of lipids, containing fatty acids, also n-3 long-chain polyunsaturated fatty acide (LC PUFA-LC PUFA), prebiotic fibres, such as fructo-oligosaccharides, inulin, galacto-oligosaccharides, gum arabic and sialo-oligosaccharides or their mixture and a probiotic bacterial strain, in particular, Lactobacillus paracasei CNCMI-2116 or Lactobacillus rhamnosus ATCC 53103 or Bifidobacterium lactic CNCM 1-3446 and additionally contains n-6 long-chain polyunsaturated fatty acid in specified quantities. At the same time the mixture of prebiotic fibres may contain 40% - 60% of gum arabic, 10% - 20% of inulin and 30% - 40% of fructo-oligosaccharide.

EFFECT: invention makes it possible to compensate for growth retardation without use of synthetic hormones and increasing calorie consumption.

5 dwg, 2 tbl, 2 ex

FIELD: chemistry.

SUBSTANCE: invention relates to biotechnology and specifically to methods of recycling household wastes. The method involves removing inorganic impurities, grinding, mixing wastes with an organic additive containing a culture of microorganisms and composting to obtain organic fertiliser. The organic additive used is compost based on poultry droppings, which is taken in amount of 300-500 kg per ton of wastes, and microbial strains Bacillus subtilis B-168, Bacillus mycoides B-691, Streptomyces sp. Ac-154, Mukor psychrophilus F-1441, Candida utilis Y-2441 in amount of 1·106-1·107 cells per millilitre per ton of poultry droppings.

EFFECT: simple process and low cost of processing household wastes.

1 tbl, 3 ex

FIELD: chemistry.

SUBSTANCE: invention relates to agriculture and can be used in processing cattle dung. The method involves formation of at least one pile of dung. Inoculating compost is added to the compost mass in amount of 10-15 kg/t. The compost mass is then moistened, biologically heated and periodically mixed. The inoculating compost used is compost based on poultry droppings and a consortium of microbial strains Bacillus subtilis B-168, Bacillus mycoides B-691, Streptomyces sp.Ac-154, Mukor psychrophilus F-1441, Candida utilis Y-2441 in amount of 1·106-1·107 cells per millilitre per ton of poultry droppings.

EFFECT: high effectiveness of the process, simple preparation of inoculating compost and low consumption of microorganisms.

2 ex

FIELD: chemistry.

SUBSTANCE: invention relates to bioconversion of poultry wastes and can be used in production of ecologically clean effective fertiliser for agricultural crops. The method involves successive layering of droppings and a water-absorbing organic material, forming at least one pile and adding microorganisms in liquid form in form of a suspension, stirring the mixture, biological heating and aerobic fermentation of the mixture. Aerobic fermentation of the mixture is carried out until temperature of the mixture naturally drops to 25-30°C. The microorganisms used are consortium of Bacillus subtilis B-168, Bacillus mycoides B-691, Streptomyces sp. Ac-154, Mukor psychrophilus F-1441, Candida utilis Y-2441 in amount of 1·106-1·107 cells per millilitre per ton of poultry droppings.

EFFECT: cutting time for processing poultry droppings and improved quality of the biocompost.

2 tbl, 4 ex

FIELD: food industry.

SUBSTANCE: aerated cultured product of camel milk contains (in ripened camel milk) biomass of a lactobacillus strains consortium including Lactobacillus acidophilus NKJC strain, Lactobacillus acidophilus JCH strain and Lactobacillus acidophilus "К3Ш24" strain in a total quantity of 104-1010 CFU/ml, their ratio in terms of CFU being 2:1:1 or biomass of a lactobacillus strains consortium including Lactobacillus acidophilus NK-1 strain, Lactobacillus acidophilus 100H and Lactobacillus acidophilus "К3Ш24" strain in a total quantity of 104-1010 CFU/ml, their ratio in CFU being 2:1:1, where content of histamine 100-800 mcmol/l, of vitamin A = 0.3-0.4 mg/l, of vitamin C = 30-60 mg/l, of vitamin B12 = 1.5-3.0 mg/l and of carbon dioxide 0.2-0.4 wt %.

EFFECT: invention ensures presence of a stable consortium of lactic acid bacteria with optimum compromise of pleasant organoleptic properties and content of useful cultured milk product ingredients in the cultured milk product of camel milk.

2 cl

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