Diagnostic technique for generalised periodontitis
SUBSTANCE: invention refers to medicine, particularly to dentistry. Diagnosis of generalised periodontitis is enabled by examining crevicular fluids by V-dehydration technique. The presence of three exactly localised facies regions makes it possible to state normal condition of periodontium tissues. The presence of arched cracks in an edge region provides diagnosing slight generalised periodontitis. The presence of arched cracks in the edge region and a band of the lower wave edge region, the absence of an intermediate region and a typical crystalline structure in the central region provides diagnosing a moderate degree. If observing a tendency to fusion of all facies regions and the presence of beam-like cracks in the edge region enables diagnosing severe generalised periodontitis.
EFFECT: technique enables diagnosing generalised periodontitis and determining its severity.
The invention relates to medicine, in particular to dentistry. Can be used to diagnose and assess the severity of generalized periodontitis.
The problem of periodontal disease is one of the major in modern dentistry and General medicine. Among periodontal disease is most common and it is generalized periodontitis. According to epidemiological studies of this disease affects about 80% of the population, it is the main cause of tooth loss. Because of this prevalence of pathology, great importance is the improvement of diagnostic methods.
- Currently one of the main methods to identify generalized periodontitis and to determine the degree of its severity, is an x-ray. (Loosejaw. Periodontal disease. 2004). It allows you to assess the condition of the bone tissue of the alveolar process. With panoramic radiographic study of periodontitis defined focus osteosclerosis, vertical type of destruction, the destruction of compact bone plate. In light of the severity of generalized periodontitis is defined by a lower altitude millionary partitions up to 1/3 the length of the tooth root, with an average severity - from 1/3 to 1/2 the length of the root, with heavy - more than 1/2.The lack of orthopantomography is that it is not always possible to obtain a clear picture of the structure of the bone tissue. The patient is subjected to radial load. This study is quite expensive, the necessary equipment is not available in each clinic. Visual analysis of radiographs, as a rule, allows to only give a subjective assessment of pathological changes in the bone tissue of the jaw. The definition of severity is complicated by the fact that the pathological process may be expressed differently in different parts of the dental arch.
The use of gingival fluid for the diagnosis of periodontal disease was first justified N.Brill and co-author. (Vshivkov. Periodontal disease. 1998). The authors proposed to use to get the gingival fluid (with intact periodontium) or content periodontal pocket standard filter paper strips. After receiving the gingival fluid filter paper strips were stained with 0.2% alcoholic solution of ninhydrin. Gambari et al. used the index gingival fluid (Tlegenova and other Diagnostics in dentistry. 2006). In addition, the authors propose a method of weighing a standard filter paper strips before and after receiving the gingival fluid on a torsion balance. To calculate the index of the selected indicators of the amount of the gum the second liquid divided by the number investigated pockets. The index value was judged on the condition of periodontal tissues (normal, chronic catarrhal gingivitis, periodontitis).
This technique requires the subsequent use of special reagents and time, as the ninhydrin stain the strip only after some time (sometimes after 1.5-2 hours depending on the temperature of the air in the room. The increase in the number of gingival fluid is most pronounced when the initial pathological changes in the periodontium. In patients with severe periodontitis increasing emphasis on the study of the qualitative composition of the gingival fluid. The methodology does not allow to assess the severity of generalized periodontitis.
- In the ways in which the study was conducted morphological composition of the gingival fluid, the increase in the absolute number of leukocytes was determined by the presence of inflammation in the periodontal tissues (Ashalata, Nigella. Theory and practice of dentistry, 1980). Inflammation and increasing the number of slomannyh epithelial cells in the gingival fluid (Tlegenova and other Diagnostics in dentistry, 2006).
When reading the contents of periodontal pockets increase the number of motile rods indicates a lesion of periodontal tissues and is directly proportional to the increase in gingival index and pocket depth (A. Grudanov, Geschreven, Lioresal. MRF, Section XII, No. 4, 1986). A more detailed study of the microflora of periodontal pockets by PCR detection of "marker" of microorganisms indicates periodontitis. Some of periodontopathogenic associated with most hard place and difficult to treat forms of periodontitis (EverEve. Rapidly progressing periodontitis, 2004). With increasing severity of periodontitis increases the frequency of detection of these microogranisms and the combination of a larger number of representatives at the same time (Aigrumae, You. Dentistry, No. 3, 2009).
In studies on the biochemical composition of the gingival fluid is established that the increase of lactate dehydrogenase activity indicates the presence of inflammation (Thielska, Enimerosi, Lahaina. Dentistry, No. 2, 1983). Activity of β-glucuronidase you can judge the severity of inflammation. In addition, there is a positive correlation between its activity, the depth of the pocket and the degree of destruction of bone tissue, therefore, and severity of periodontitis (Gambari, VII, Ashalata. Dentistry, No. 4, 1986). The degree of inflammation in the periodontal tissues can be judged by the increased activity of acid and alkaline phosphatase gingival fluid. Its activity correlates with the depth of periodontal pockets (IshikawaJ., Cimasoni G. Arch. Oral Biol., 1970). The degree of inflammation in the periodontal tissues can be judged by the concentration of endotoxins (lipopolysaccharides) of microorganisms (Gambari, VII, Ashalata. Dentistry, No. 4, 1986).
All these methods of diagnostics on the composition of the gingival fluid is often difficult to implement, require expensive equipment and specially trained personnel. To get results sometimes requires a significant amount of time, and the result is tentative. For example, direct microscopic examination gives only approximate, rough results, and the cultivation of periodontopathogenic is complicated by their high sensitivity to oxygen and the duration of the study. All these methods allow to describe only one indicator of gingival fluid when it is impossible to assess the overall condition of periodontal tissues. Often there is no opportunity to assess the severity of periodontitis.
- The closest is a method of diagnosing pathologies of the oral cavity by crystallographic picture of oral fluid obtained by the method of the wedge dehydration. The essence of the method consists in drying drops of the oral fluid analysis and morphological patterns obtained facies using microscopic examination. If the process were obtained facies oral liquid is ti and the regularities of their morphological patterns in persons with "natural hygiene", sanitized and pathology of the oral cavity (Snezana Diagnostic and prognostic criteria of dental pathology on morphological picture of oral fluid in patients of different age groups: author. dis ... Prof. the honey. Sciences). In facies oral fluid of patients with healthy periodontium observed a clear system structureborne divided into two zones: the regional amorphous and Central crystal. The systematic organization of oral fluid of patients with pathology of the oral cavity in all cases there were clear differences from facies oral fluid of individuals with a healthy periodontium. Facies fully had the appearance of chaotic substance or chaotic substance occupying most part of it.
The disadvantage of this method is that, allowing to determine the presence of pathology of the oral cavity, the method does not allow differential diagnosis of caries, gingivitis, periodontitis and diseases of the mucous membranes of the oral cavity. It is not possible to determine the severity of the pathological process. Substances that have important diagnostic value in the pathology of the periodontium, oral fluid contained in a large dilution, therefore, negligible amount.
We were the first accurate and informative method for the diagnosis of generalized periodontitis by Chris holographically the film gingival fluid. This method allows to diagnose generalized periodontitis and to determine the degree of its severity. The research material is selected gingival fluid, because it has exceptional diagnostic value. She washes directly periodontal tissues and can most accurately describe their condition.
The method consists in the following.
For fences of the gingival fluid used paper pins No. 30. The pin is placed in the gingival sulcus or periodontal pocket. Gingival fluid at a healthy periodontium collect in the area of the teeth 16, 11, 26, 31 - vestibular surfaces 36, 46 - lingual surface. Chronic generalized periodontitis gingival fluid take in the parts of the dental arch with the most severe pathological changes. After sampling the pin is placed in 40 μl of solvent. As the solvent used distilled water. The volume of solvent was found experimentally, the criterion of selection was the most distinct crystallographic picture facies of the gingival fluid. The material is then centrifuged at 3000 rpm for 10 minutes. On a thin glass slide semi-automatic dispenser is applied supernatant (six drops of each sample) in an amount of 5 μl. The glass is placed in a thermostat at 30 min at 37°C. a total of n the whole study takes about 45 minutes. At the completion of the dehydration receive facies of the gingival fluid, which is subjected to microscopic examination.
When carrying out the work were examined 34 patients, individuals with a healthy periodontium, and diagnosed with chronic generalized periodontitis of varying severity exacerbation. Clinical examination of patients in addition to medical history and examination included determination of the depth of periodontal pockets, the degree of mobility of the teeth, x-ray (orthopantomogram). Assessment of dental status was made on periodontal indices (index of oral hygiene Green J.C.Vermillion J., index ACA, periodontal index, Russel). Material was collected after 3-5 hours after brushing, provided that the individual did not consume food in a specified period of time.
In subjects with clinically healthy periodontal tissues value periodontal indices were within normal limits (hygiene index Wiig - 0-0,6; PMA - up to 30%; PI 0-0,2). Us was first obtained crystallographic picture of the gingival fluid in the norm (figure 1). This clearly defines three characteristic zones facies. The regional area during dehydration of the gingival fluid in normal looks like an amorphous area that does not contain any inclusions (1). The regional area is homogeneous, in the bottom part throughout environment the particular facies has a uniform darker strip (2). The staging area is well expressed (3). OK can distinguish the following morphological feature of the intermediate zone is the presence of two distinct zones. The first zone (closer to the edge) is relatively narrow and presents small crystal formations in the form of a cross (4). The second zone is wider (5), presents a larger right crystals having the form of a fern or crosses (which is less common). These skeletal structures represent the main form of crystals facies of the gingival fluid. The largest area of the Central zone consists of many small crystalline structures having a grid view (6).
Chronic generalized periodontitis showed characteristic clinical picture: pathological tooth mobility, the presence of periodontal pockets, higher values of the indices of hygiene and periodontal indices. On the x-ray destruction of the cortical plate, the centers of osteoporosis, decreased height millionary partitions. All research results were consistent with the severity of generalized periodontitis. When mild severity (figure 2) in crystallographic picture of the gingival fluid are determined by all three zones facies: regional (1), intermediate (3) and Central (6). In the marginal zone are arcuate fractures (7). When media is her severity (figure 3) in the marginal zone (1) throughout the duration determined by the arcuate elements (7). Band (2) in the lower part of the marginal zone is rugged, has the form of waves. The intermediate zone is not defined. In the Central zone there is no typical crystal structure.
The more severe the degree of the pathological process (figure 4) is characterized by the provision of the morphological picture of the destroyed almost completely. There is a tendency to merge all zones facies. In the marginal zone is characterized by the presence of cracks, which have the form of rays (8).
Clinical example. Patient, 45 years old, came with complaints of intermittent bleeding gums, sore gums, bad breath, and tooth mobility. Sick the last 6 years. During the inspection revealed that the presence of dental plaque: soft plaque, above - and subgingival calculus. The mucous membrane of the gums cyanation.
Pathological tooth mobility I-II degree. In the area of the teeth 31, 32, 34, 35, 41 and 42 are determined by the depth of periodontal pockets of 4-5 mm and traumatic occlusion. The hygiene index Wiig - 1,66; PMA - 47%, PI - 3,31.
On the orthopantomogram is determined by the destruction of the cortical plates of interdental septa, vertical type and the destruction of bone, decreased height millionary partitions up to 1/3, in the area of the teeth 31, 32, 34, 35, 41 and 42 to 1/2 the length of the roots of teeth, enlargement of the periodontal gap in the cervical area of the teeth 34, 35.
In the study of morphological pattern disnev the second fluid (figure 5) clearly defined regional area facies (1), which contains the arcuate fractures (7). Dark band (2) marginal zone is uneven, has the form of waves. Typical norms crystal picture intermediate and Central zones is missing.
In this example, the crystallographic picture of the gingival fluid is fully consistent with the clinical picture of moderate severity of periodontitis.
The crystallographic study of the painting of the gingival fluid can be more accurately describe the condition of periodontal tissues, allows you to catch the slightest changes. Pathological processes in the periodontium even at the initial stages of development there are always changes in physico-chemical properties of the gingival fluid. These primary changes primarily occur at the molecular level. The information contained in the gingival fluid at the molecular level, when dehydration is reflected in the different crystal structures. Therefore, this method is extremely sensitive and informative. The method is simple, it does not require expensive equipment and specially trained personnel. In addition, this method allows to install and severity of generalized periodontitis. Crystallographic picture facies of the gingival fluid can give complete information about the General condition of periodontal tissues. Therefore, the investigation of crystal structures on Savoy fluid is of great theoretical and practical importance for Periodontology.
Method for the diagnosis of generalized periodontitis method wedge dehydration, characterized in that examine the gingival fluid and in the presence of three clearly delimited zones facies set the normal state of periodontal tissues; in the presence of the arcuate cracks in the edge zone diagnose mild generalized periodontitis; in the presence of the arcuate cracks in the edge zone and strip the lower part of the marginal zone having the form of waves, the absence of the intermediate zone and the typical crystal structure in the Central zone diagnose medium; when observing a tendency to merge all zones facies and the presence in the marginal zone of cracks, which have the form of rays, diagnosed with severe generalized periodontitis.
SUBSTANCE: device is comprised of the first and second part. The first part contains the edge piercing the membrane, capillary channel and a hole in the outer surface of the first part. The second part comprises the chamber for processing solution and the membrane closing the chamber for processing solution. Wherein the hole is opened when the first part of the device is initially put into the second part of the device, and the hole is closed when the first part of the device is fully put into the second part of the device. Capillary channel made in the first part of the device is sized for receiving the liquid medium sample. The second part is comprising the chamber for processing solution that can be limited by first and second membranes, wherein the first part gets through the first membrane when the first part is put into the second par in such a way that the content of processing solution chamber is mixed with the content of capillary channel. The tool for piercing the second membrane is made in such a way that the content of processing solution chamber and capillary channel can be forced out of the device. The device can also comprise the plunger with the mechanism for getting through the second membrane in such way that the content of processing solution chamber and capillary channel is forced through the plunger.
EFFECT: increased pressure control in the device when supplying necessary amount of sample into analyzing cartridge.
4 cl, 16 dwg
FIELD: tritium production.
SUBSTANCE: invention relates to the method and device for automatic tritium extraction from atmospheric water vapour through the use of a freeze trap. The method comprises the first stage of condensing water vapour by cooling a section of the freeze trap, and the second stage of recuperating the ice formed during the first stage into liquid condensate. The air is contained within the extraction case which is used to remove water from the extracted air; the cooling section of the freeze trap is also positioned within the extraction case. During the first stage the freeze trap, which is kept at a temperature below 0° C, is activated during a specified period of time with the possibility of ice formation on the freeze trap cooling section. During the second stage the previously cooled section of the freeze trap is heated by stopping the freeze trap cooling, and liquid condensate, which is formed after ice melting, is collected. The device comprises a movable case for air extraction, which includes a pipe coil connected through a pump with an external liquid nitrogen tank, and a water tank positioned under the coil pipe.
EFFECT: device responsiveness improvement; sampling time reduction; prevents contamination of one sample by another sample.
9 cl, 1 dwg
FIELD: engine maintenance.
SUBSTANCE: invention relates to the non-destructive inspection through the radiography of a gas turbine engine blade made from composite materials. A control sample of a blade is manufactured from composite materials to standardise the radiographic inspection of such blades. A three dimensional raw piece is braided from composite materials and elements made from resin are positioned in certain points of the piece. The raw piece is then placed into the casting box and then resin is fed to the casting box to produce the control sample of the blade.
EFFECT: standardisation of the engine blade radiographic inspection for a distinctive flaw - accumulation of the resin inside the blade.
4 cl, 5 dwg, 1 ex
SUBSTANCE: method involves collection of initial plankton samples from the water body under investigation, and preparation thereof, said samples containing hydrobionts-bioindicators of radioactive contamination. The resultant samples of said hydrobionts are prepared and analysed, followed by evaluation of the radiation condition of the water body under investigation. The hydrobionts-bioindicators of radioactive contamination of the water body used are sea zooplanktons of the Chaetognath type. One initial plankton sample containing said hydrobionts is collected at any arbitrary or directed given point of the offshore zone under investigation with salinity not lower than 8%. One resultant sample of said hydrobionts is then prepared by collecting from the initial sample not less than 5 zooids of sea zooplanktons of the Chaetognath type. Analysis of the resultant samples of said hydrobionts is carried out through visual examination of outer morphological features which characterise the state of the skin and fins of each separately taken zooid from among those collected. The radiation condition of the investigated offshore zone is carried out by detecting presence or absence from the resultant sample zooids of said hydrobionts, having anomality of said outer morphological features. If the resultant sample has at least one zooid, having damaged skin and/or broken fins, the offshore zone under investigation has radioactive contamination.
EFFECT: simple and shorter method, reduced expenses on implementing the method.
9 cl, 2 ex
FIELD: machine building.
SUBSTANCE: proposed device comprises sealed work chamber, stand, baths, pans, work table accommodating stations with bath positioning devices, pans, movable stand drive equipped with gripper, lifting mechanism and drive, and control unit. Automatic device incorporates also sealed work chamber with sealing side, drying chamber, driven rotation mechanism, stand with "П"-shape handle, stand base is rounded and skewed at 20÷70°. Stand sidewall inner surface supports separators made up of a comb with ledge thickness of 1÷4 and spacing width of 1.1÷2.0 of microscope slide thickness. Movable stand drive incorporates gripper made up of rectangular-section bucket. Note here that gripper moves along circular arc within 0÷40°. Baths and pans are provided with eyelets. Stations are equipped with positioning devices made up of cone pairs with truncated apex and cylindrical location flange, its diameter making 0.80÷0.95 of eyelet diameter. Aforesaid "П"-shape handle has length making 1.1÷1.2 of relation between gripping height and drive arm lifting angle cosine. Bath wall skew makes 5÷20°, wall facing stand drive.
EFFECT: higher efficiency and reliability, ruled out fouling.
1 tbl, 4 dwg
SUBSTANCE: apparatus for stationary collection of water in the ocean bottom layer has a ballast weight on which a water collection chamber is mounted, a pipe made in form of a reinforced hose and connected with the water collection chamber, a buoy to which the top end of the pipe is attached through a sleeve nut, floats and a flexible hose which freely passes through the opening in the float and connected to the buoy. An annular magnet is mounted in the float, and inside the flexible hose there is a cylindrical magnet which is tightly fit to the walls of the house, where the axial magnetic field of the cylindrical magnet is opposite the field of the annular magnet.
EFFECT: invention enables to regularly monitor physical and chemical properties of water at any depth at stationary points with constancy of all collection conditions with low expenses.
1 cl, 2 dwg
FIELD: oil and gas industry.
SUBSTANCE: sampling method of fluid from pipeline, at which sampling element with sampling hole is arranged in pipeline and sample is taken through the inlet with the specified flow. At that, passage of sampling hole is increased by the coefficient inversely proportional to 0.6-1, and sample is taken from the flow in the pipeline at average velocity proportional to average flow velocity in the pipeline with proportionality factor from interval of 0.6-1. Pipeline fluid sampling device includes sampling element installed diametrically in the pipeline with the sampling hole oriented towards the flow in the pipeline and made on side surface of sampling element; at that, passage of sampling hole is made in a certain way.
EFFECT: invention allows increasing representativity of the sample, improving the pump operation and reducing electric power consumption.
2 cl, 3 dwg, 1 tbl
SUBSTANCE: mechanical ice drilling bit includes housing on the edge of which detachable cutters are fixed, which are located symmetrically in radial direction where the cutting edge of cutters is turned inward the bit, and core breaker. Cutting edges of cutters are offset relative to each other in radial direction without overlapping each other and equal to 1/n of the cutter width, where n - the number of the bit cutters.
EFFECT: increasing mechanical drilling speed, increasing run drilling, reducing the power consumption of the cutting process owing to decreasing the thickness of the cut layer with each cutter.
SUBSTANCE: invention refers to medicine, particularly to cardiovascular surgery. To forecast late thrombotic reocclusions, biopsy of abdominal aorta portion from the area of proximal anastomosis is performed. Further histological research is performed with morphometric changes of the following parameters: macrophage presence, intima state, inclusion properties, muscular element state. Probability of late thrombotic reocclusions (W) is determined by the formula W=A×B×C×D×100 where A is probability of thrombotic reocclusions for patients with various macrophage presence levels; B is probability of thrombotic reocclusions for patients with various intima defect levels; C is probability of thrombotic reocclusions for patients with various inclusions in vascular walls; D is probability of thrombotic reocclusions for patients with various state of vascular wall muscle elements. For W within 1.1 to 5% high risk of late thrombotic reocclusions is diagnosed, for W within 0 to 1% low risk of late thrombotic reocclusions is diagnosed.
EFFECT: increased accuracy of late thrombotic reocclusion forecast in post-operation period due to involvement of morphological parameters characterising disease combativeness in operation area.
2 ex, 1 tbl
SUBSTANCE: delimitation of a high-grade glioma invasion is ensured by imaging of an astroglial bank surrounding the high-grade glioma. An immunogenic recombinant human GFAP is prepared and used to immunise a Balb/C mouse; spleen B-lymphocyte of this mouse are recovered and fused with myeloma cells of Sp 2/0-Ag14 mice; hybridomas are produced. Supernatants of the prepared hybridomas are tested by immunochemical techniques for the presence of anti-GFAP antibodies used to select a hybrid cell clone producing the anti-GFAP antibodies able to distinguish GFAP in vivo. The anti-GFAP antibodies are cleaned from the supernatant of the selected clone and covalently bound with liposomal nanocontainers containing a diagnostic mark. The antibodies of the selected hybrid cell clone is modified by g-amino groups of lysine residues and incubated with the stelths-liposome solution. The prepared nanosystem is introduced in a patient's vascular bed, and the astroglial bank is imaged by the arrangement of the diagnostic mark in cerebral tissues.
EFFECT: method allows preventing relapses of high-grade gliomas after their surgical management by choosing an optimal extent of a pending surgery ensured by delimitation of a tumour invasion by means of imaging of the astroglial bank.
6 cl, 4 dwg, 1 ex
SUBSTANCE: invention refers to medicine, particularly oncology, endocrinology and X-ray diagnostics. For the purpose of evaluation of the propensity for high breast density in postmenopausal women, blood is examined for five parameters: reactive insulinemia, relation of generation of active oxygen forms in mononuclears to reactive insulinemia, glyoxalase activity in mononuclears on an empty stomach, relation of glyoxalase activity at 120-min oral glucose tolerance test to glucose activity on an empty stomach and an amount of blood circulating stem cells CD90+. Each parameters is appraised by points. If score is ≥ 4, the propensity for high breast density is concluded.
EFFECT: method enables optimising an approach to describing a group of postmenopausal women prone for high mammography breast density based on evaluation of laboratory parameters showing potential mechanisms of such high mammography breast density associated with weakened endocrine and intensified progenotoxic glucose effect and lower blood content of stem cell CD90+ marker showing according to some reports a suppressor function of a malignant process.
SUBSTANCE: invention relates to medicine, namely to urology, and can be used for predicting terms of recovering function of kidneys in patients with hydronephrosis in case of urinary tract obstruction. Clinical examination is carried out and risk factors are determined: causes and degree of hydronephrosis, presence of post-operative complications, duration of hydronephrosis disease, effective kidney plasma flow, parenchimatose index and time of maximal rise of renohistogram curve. Determined risk factors are given gradations and numerical values, after which terms of recovering function of kidneys in months (Y) is calculated by formula: Y=8.83-4.56X1+3.18X2+3.64X3+14.01X4+0.01X5-0.32X6+0.19X7 where X1,2,7 are gradations and numerical values of risk factors, X1 are causes of hydronephrosis: cervical cancer - 1, stenosis of pelvic-ureteral segment - 2, nephroptosis - 3; X2 is hydronephrosis degree: II -2, III - 3; X3 is post-operative complications: no - 0, yes - 1; X4 is duration of hydronephrosis duration: to 3 years - 0, from 3 to 5 years - 1, more than 5 years - 2; X5 is effective kidney plasma flow after operation, ml/min; X6 is parenchimatous index after operation, units; X7 is time of maximal rise of renohistogram curve after operation, min.
EFFECT: method provides possibility to predict expected term of recovering function of kidneys in months in patients with hydronephrosis
SUBSTANCE: invention relates to field of molecular biology, clinical biochemistry, medicine, veterinary, pharmacology, endocrinology and oncology. To diagnose prostate cancer, patient's blood is sampled. Sample preparation of blood is performed aimed at its deproteinisation. Sedimentation of proteins from analysed fluid is carried out with methanol, after which direct mass-spectrometric analysis of fractions of analysed substances - markers - is carried out by quadrupole-time-of-flight mass-spectrometry.
EFFECT: method makes it possible to diagnose prostate cancer.
1 dwg, 3 ex, 1 tbl
SUBSTANCE: method relates to field of medicine, namely to obstetrics. To diagnose early hypogalactia analysis of colostrum is carried out. On the 3-4-th day of postpartum period, in centrifuge tube mixed are equal quantities of colostrum and 15-20% of SAS solution by 3-4-time turning-over the tube for 4-5 seconds. If column of foam is formed, early hypogalactia is diagnosed.
EFFECT: method makes it possible to diagnose hypogalactia at early terms.
SUBSTANCE: invention relates to field of medicine, namely to infectious diseases. To predict interferon-ribaflavin-induced neuropenia in patients with chronic hepatitis C, obtaining combined antiviral therapy (CAT), phenomenon of induction of neutrophil apoptosis in vivo, determined by "skin window" technique, is used. Before administration of CFT to patients in impression-smears determination of induced neutrophil apoptosis is carried out. As apoptosis inductor, used is solution of 0.2 g of Rebetol and 1.7 mln IU Altevir, diluted in 1.0 l of isotonic solution. If in "skin window" high values of induced neutrophil apoptosis ≥40% is determined, high risk of neutropenia development in said category of patients in case of future carrying out to them CAT is predicted.
EFFECT: method makes it possible to predict interferon-ribaflavin-induced neuropenia in patients with chronic hepatitis C due to application as neutrophil apoptosis inducer of isotonic solution, consisting of rebetol and altevir, in vivo by "skin window" technique.
3 ex, 1 tbl
SUBSTANCE: invention relates to field of medicine, namely to infectious diseases, hepatology. To carry out differential diagnostics of HBV- and HCV-infection in patients with liver cirrhosis analysis of blood serum is carried out. In the same sample of blood serum content of antithrombin-III and plasminogen level is determined. With simultaneous values of level: antithrombin-III 60-80%, plasminogen 45-55%, liver cirrhosis of viral etiology HBV is diagnosed. With simultaneous values of levels of antithrombin-III 50-59%, plasminogen 56-65%, liver cirrhosis of viral etiology HCV is detected.
EFFECT: method makes it possible to differentiate liver cirrhosis of HBV-etiology from liver cirrhosis of HCV-etiology.
2 ex, 1 tbl
SUBSTANCE: invention relates to medicine, namely to infectious diseases. In order to predict version of HIV-infection course in children in child's blood tested are quantity of CD-4-lymphocytes and HIV viral loading of blood (VLB). Additionally tested are mother's anamnestic indices: age, intake of drugs, chronic metroendometritis, chronic vaginitis, HIV-infection, gestosis during pregnancy, threat of pregnancy abortion, chorionamnionitis during pregnancy, fetoplacental insufficiency, labour. Child's anamnestic indices are analysed: inborn development defects, retardation of intrauterine development, jaundice in the newborn period, intrauterine infection different from HIV, generalised lymphadenopathy, hepatomegalia, splenomegalia, body weight, mucosa candidosis, acute respiratory infections, pneumonia. Each mothger's anamnestic index, as well as child's anamnestic index, in case of their presence, are given points. If the sum of points is not lower than "plus" 13, quick version of HIV-infection course in children infected with HIV by vertical way, is predicted.
EFFECT: method increases reliability of predicting version of HIV-infection course in children, infected with HIV by vertical way.
1 ex, 1 tbl
SUBSTANCE: invention relates to field of medicine. In order to diagnose metabolic syndrome it is found out if abdominal obesity, arterial hypertension, hyperglycemia on the empty stomach, hypertriglyceridemia, hypoalphacholesterinemia, hyperuricemia, non-alcohol fatty liver disease are present. Additionally determined is excessive body weight or obesity independent on type of fat deposition, insulin-resistance, hypercholesterinemia. Degree of each component expression is determined in points. Metabolic syndrome, its degree of severity and degree of risk of development are diagnosed by the sum of points, as well as by presence of necessary set of components.
EFFECT: method makes it possible to diagnose metabolic syndrome, degree of risk and degree of severity of its development.
14 ex, 1 tbl
SUBSTANCE: invention relates to field of medicine, namely to cardiology. In order to estimate risk of development of cardiovascular events in patients with ischemic heart disease, subjected to endovascular myocardium revascularisation during 1 year after interference carried out is estimation of the level of proinflammatory cytokines IL-6 and FNOα, cholesterol of low-density lipoproteins of blood serum, triglycerides, cholesterol of high-density lipoproteins, fact of tobacco-smoking and non-intake of statins is taken into consideration. If more than four taken into account factors: initial level of are present proinflammatory cytokines IL-6 and FNOα is higher than 5 mg/ml and FNOα, higher than 50 ng/ml, cholesterol of low-density lipoproteins higher than 2.5 mmol/l, cholesterol of high-density lipoproteins lower than 1.0 mmol/l, triglycerides higher than 1.7 mmol/l, smoking, non-intake of statins, patients are referred to category of high risk of development of unfavourable cardiovascular events.
EFFECT: method makes it possible to predict development of unfavourable cardiovascular events in patients with ischemic heart disease during 1 year after endovascular myocardium revascularisation by quantitative estimation of the most sensitive factors of risk of cardiovascular diseases.
SUBSTANCE: invention relates to medicine, namely, to diagnostics of cardiovascular diseases. Ultrasonic location of heart and vessels is carried out. As contrasting substance used is stable suspension of particles of ultradispersive and homogeneous nanopowders of metal oxides obtained by method of electric explosion of conductors, size of nanoparticles constituting not more than 200 nm, minimal value of volume concentration of particles being 1% (9vol). Computer processing of images is carried out with application of visualisation algorithms.
EFFECT: method improves visualisation of heart and vessels due to application of suspensions of particles of nanopowders of metal oxides and increases quality of diagnostics.
3 cl, 14 dwg, 3 tbl
FIELD: medicine, clinical toxicology.
SUBSTANCE: at patient's hospitalization one should gather the data of clinical and laboratory values: on the type of chemical substance, patient's age, data of clinical survey and laboratory values: body temperature, the presence or absence of dysphonia, oliguria being below 30 ml/h, hemoglobinuria, erythrocytic hemolysis, exotoxic shock, glucose level in blood, fibrinogen and creatinine concentration in blood serum, general bilirubin, prothrombin index (PTI), Ph-plasma, the state of blood clotting system. The state of every sign should be evaluated in points to be then summed up and at exceeding the sum of points being above "+20" one should predict unfavorable result. At the sum of "-13" prediction should be stated upon as favorable and at "-13" up to "+20" - prediction is considered to be doubtful.
EFFECT: higher accuracy of prediction.
2 ex, 3 tbl