Method for detection of enteroviruses in water
SUBSTANCE: enteroviruses are concentrated by introduction into an analysed water sample of a magnetic sorbent microparticles coated with polymeric silicon dioxide with amino poropyl groups in proportions 1:1000-3000 of water sample volume. It is incubated at constant stirring for 1-2 hours. The sorbent is collected with a magnet, a supernatant is removed, and a sorbent-enterovirus complex is produced. Enteroviruses are eluted by 0.5M NaCl and 0.05M Tris (pH-10.5) solution. Enteroviruses are identified by immunochemical, cultural and molecular methods.
EFFECT: high degree of virus concentration in the eluate, decreased amount of the eluating solution.
The invention relates to the field of medicine, epidemiological surveillance and Virology, and can be used to detect enteric viruses in tap water, in the water, open water, freshwater and seawater, water from underground sources, bottled drinking water, in the water of swimming pools and waste water.
The level of technology.
At the present time to detect enteric viruses in water in the Russian Federation there are different ways, each of which applies to a specific type or volume of water. This method using filtration membranes using ion-exchange resins, two-step method (sorption on ion-exchange resin and precipitation using ammonium sulfate), the method of two-phase separation method using non-woven bags with makroporistom glass and using the kit for collection and concentration of viruses from drinking water using trap device [HOWTO No. 4.2.2029-05 "control Methods. Biological and microbiological factors. Sanitary and virological monitoring of water objects"].
There are also known methods, proposed by foreign authors. This concentration of viruses on membranes with ligands [US patent US 20080014625], negatively charged membranes [Katayama H., Shimasaki A., Ohgaki S. Development of a virus concentration method and its application to detection of nterovirus and Norwalk virus from coastal seawater. Appl. Environ. Environ., 2002, 68: 1033-1039].
Closest to the claimed method is a method of detection of enteric viruses, including the concentration of viruses by using non-woven bags with macroporous glass [Kontorovich V.B. have been, Ivanova PU, Eremeeva SO, Shearman GA, V.A. Kazantsev Method for the concentration of viruses in the water environment. The matters. virusol., 1996, 1: 40-42]. In the specified way time concentration of viruses from water surface water and waste water is 3-7 days. To improve the sorption properties of macroporous glass, representing the white powder, it is treated as follows: one volume of glass is poured into the flask one volume of a mixture (1:1) 3% solution of H2O2and 6M hydrochloric acid and boil in a fume hood for 1 h without tube, observing precautions. Washed a large amount of distilled water until neutral pH and dried at 100°C. In a batch of fabric measuring 5×7 cm was placed 3.0 cm3the prepared sorbent. Package sorbent is fixed by means of a scaffold for a motionless subject so that he was in the water flow. After exposure within 3-7 days package take out, put in some new plastic bag or sterile vial and transported to the laboratory in a refrigerator bag in the shortest with the OK (no more than 6 hours). Each sample is labeled indicating the point of selection, installation date, and time of exposure of the package. Before processing samples can be stored for days at 4°C. the Package with the sorbent is removed from the shipping container and placed in a sterile Petri dish. Cut the edge of the package, wash the glass with distilled water (5 ml) using a pipette in the same Petri dish and transferred by pipette or through the funnel into the column in a volume of 5-10 ml elute Viruses step three solutions in 3 ml each, collecting fractions in separate penicillin vials. Research expose each fraction separately (all 3 factions). As eluting solutions are used: 1) 0.05 M Tris-HCl pH 9,1; 2) 0.05 M Tris-HCl pH 9.1 and 0.5 M NaCl; 3) 3% beef extract in 0.05 M Tris-HCl pH to 9.1. For detection of viruses in the eluate choose culture, immunohistochemistry or molecular methods.
The disadvantages of the above method are its duration, a large number and volume fractions of the eluate, a small area of contact of the sorbent with water volume.
The present invention, which is directed technical result is to reduce the duration of the claimed method and its simplification. In addition, there is a high degree of concentration of viruses, decreases the volume of eluting solution and, consequently, increases the output is irusa in the eluate by using as adsorbent magnetic particles, polymer coated silicon dioxide with aminopropylene groups.
To achieve the technical result in the method of detection of enteric viruses, including the concentration of viruses from water samples on the sorbent, the elution of enteric viruses from sorbent, followed by immunochemical detection, culture or molecular methods, wherein the concentration of enteric viruses is carried out by depositing in the analyzed water sample sorbent on the basis of magnetic microparticles coated with the polymer of silicon dioxide with aminopropylene groups in the ratio of 1:1000-3000 the volume of the water sample, incubation with constant stirring for 1-2 hours, collecting the sorbent using a magnet, remove the supernatant and obtain complex sorbent with intestinal viruses, and the elution of enteric viruses carry out the solution of 0,5M NaCl and 0,05M Tris (pH of 10.5).
Features that distinguish the present invention from analogues are:
the time of incubation of the sorbent MagSi+ sample - 1-2 hours;
- sorbent MagSi+ presents suspension, which increases the probability of adsorption of viruses due to the uniform distribution of the sorbent in the whole volume of water;
- magnetic properties MagSi+ it is possible to collect the sorbent using the magnet in vials or flowing systems;
a positive charge MagSi+ in water which allows it to adsorb viruses not only due to hydrophobic interactions, but due to ion;
- elution of viruses from the sorbent is a small amount of eluting solution, which increases the concentration of viruses in the eluate;
composition and pH value of an eluting solution.
Disclosure of the invention.
The method of detection of enteric viruses in water is the concentration of water on magnetic microparticles coated with the polymer of silicon dioxide with aminopropylene groups, the removal of virions from the sorbent using a special buffer for elution and subsequent detection of enteric viruses in the eluate using molecular, immunochemical or cultural methods.
Sorbent MagSi+ was obtained according to the method described in U.S. patent US 20030148101 A1 with some modifications. The coated magnetite was carried out in two stages where the first stage was identical to that described in the patent (polymerization of tetraethoxysilane on the surface of particles of magnetite at pH 4.6 and a temperature of 90°C), and at the second stage the solution of tetraethoxysilane was replaced with 3-aminopropyltrimethoxysilane in the same concentration. The reaction on the surface of the magnetite formed polymer layers of silicon dioxide with aminopropylene groups that at pH lower than 7.0 have a positive charge. The sorbent is a particle dark brown color size 2-10 μm, not prone to the formation of aggregates is determined that same positive charge particles. The capacity of the sorbent MagSi+estimated according to the model protein BSA (bovine serum albumin)is 15-20 mg of protein per 1 cm3sorbent. In model experiments for concentrating viruses from 1 l of tap water efficiency concentration of adenovirus was 76%, enteroviruses - 81% and of hepatitis a virus - 88%.
The principle of adsorption of viruses on the sorbent MagSi+ based on two types of interactions: (1) hydrophobic interaction between the capsid proteins of viruses with polysiloxane bonds of the polymer silicon dioxide; (2) electrostatic interaction between the amine groups of the sorbent, which in aqueous conditions at pH lower than 7.0) have a positive charge, with virions, negatively charged at pH values above the 5.0. In addition, the same positive charge particles MagSi+ provides the distribution of the sorbent in the whole volume of water, thereby increasing the likelihood of exposure of virions with particles of sorbent and speeding up the process of sorption. Thus, to ensure the effective concentration of viruses due to electrostatic interactions it is necessary that the pH of the water was in the range of 5.0-7.0. This pH range corresponds to the average values of the pH of the water.
The method includes the following steps.
1. The introduction of sorbent in a water sample. Suspension of sorbent MagSi+ in a volume of 1 ml (1:1 by volume sorbent MagSi+ and 50 mm races the thief NaCl) is added to 1 l of the investigated water.
2. Incubation with constant stirring for 1-2 hours at room temperature. Mixing is carried out using a shaker or verhneprivodnaya stirrers, the entire sorbent must be suspended.
3. Collection of sorbent MagSi+ using magnet in vials or in special units for flow concentration of magnetic microparticles. Remove supernatant, obtaining complex virus + sorbent.
4. Elution of viruses is the solution of the following composition - 0,5M NaCl, 0,05 m Tris, pH of 10.5, based on 1 ml of the suspension of sorbent MagSi+ added 1 ml of an eluting solution.
5. Indication and identification of viruses using immunohistochemistry, culture or molecular methods. For the detection of rotavirus and hepatitis a virus applies the detection of viral antigens and enzyme-linked immunosorbent assay. Cultural methods, in particular, the neutralization reaction, used for the detection and typing of enteroviruses (ECHO, Coxsackie a and b, Polioviruses and Enteroviruses 68-71) and adenoviruses. Molecular techniques such as reverse transcription and subsequent PCR or PCR "real-time"can be used to identify all known enteric viruses [Svraka S et al. 2009, G. Shay Fout et al. 2003; guidelines No. 4.2.2029-05].
The implementation of the invention.
Prima is 1.
Carry out the concentration of enteric viruses from wastewater samples. To 1 l of pre-clarified waste water add 1 ml of the suspension of the sorbent MagSi+. Incubated with constant mixing on a shaker or using verhneprivodnaya stirrer for 1 h After exposure collecting the sorbent using a rare earth magnet, transfer the suspension into a 15-ml sterile polypropylene tube. Remove supernatant, holding magnet sorbent with adsorbed viruses on the inner wall of the tube, and hold the elution of viruses 1 ml of a solution of the following composition - 0,5M NaCl, 0,05M Tris, pH of 10.5. Intensively shake the test tube on a vortex for 15 minutes, the entire sorbent must be suspended. Precipitated sorbent by centrifugation at 3000 rpm for 10 minutes, or by using a magnet. The supernatant transferred to two 1.5-to 2.0-ml polypropylene tubes with screw cap. Indication and identification of viruses and their structural elements (viral proteins or nucleic acids), make use of immunohistochemistry, culture or molecular methods (various modifications of the PCR).
Carry out the concentration of enteric viruses from water (drinking) water. To 10 l water (drinking) water, add 3 ml of the suspension of the sorbent MagSi+. Incubate the ri constant mixing on a shaker or using verhneprivodnaya mixer 2 hours After exposure in water add 100 ml 5M NaCl and incubated with stirring for another 30 minutes. Collect the sorbent using a rare earth magnet, transfer the suspension into a 15-ml sterile polypropylene tube. Hold the sorbent magnet, remove the supernatant and spend the elution of enteric viruses in 3 ml of a solution of the following composition - 0,5M NaCl, 0,05 m Tris, pH of 10.5. Intensively shake the test tube on a vortex for 15 minutes, the entire sorbent must be suspended. Precipitated sorbent by centrifugation at 3000 rpm for 10 minutes, or by using a magnet. The supernatant in 1.5 ml transferred to two 1.5-to 2.0-ml polypropylene tubes with screw caps. Indication and identification of enteric viruses or their structural elements (viral proteins or nucleic acids), make use of immunohistochemistry, culture or molecular methods (various modifikacii PCR).
The method of detection of enteric viruses, including the concentration of viruses from water samples on the sorbent, the elution of enteric viruses from sorbent, followed by immunochemical detection, culture or molecular methods, wherein the concentration of enteric viruses is carried out by depositing in the analyzed water sample sorbent on the basis of magnetic microparticles coated with poly is a leader in silicon dioxide with aminopropylene groups, in the ratio of 1:1000-3000 the volume of the water sample, incubation with constant stirring for 1-2 hours, collecting the sorbent using a magnet, remove the supernatant and obtain complex sorbent with intestinal viruses, and the elution of enteric viruses carry out the solution of 0.5m NaCl and 0,05M Tris (pH of 10.5).
SUBSTANCE: fixed and mobile monitoring sites equipped with measuring instrumentations are located. Various environmental parameters are registered and subjected to analysis. More specifically, hydrophysical field signals are being registered, chemiluminescence, chromatographic, ion-selective, spectral and radiometric analysis is performed. Besides, bed acoustic impendance is registered, molecular spin interactions of seawater protons are detected, artifacts resulting from the magnetohydrodynamic, bioelectric and concentration effect are detected, synthetic surfactant content in the aquatic environment, chlorophyll concentrations, microorgasnisms, phytoplankton, zooplankton is determined. The collected data is further transferred to the archivers and modeling is performed. In the course of modeling the industrial facility environment and infrastructure is divided into a number of areas and a material balance model and a forecast model are created for each of them. For the purposes of the method implementation a system comprising a water withdrawal line equipped with hydrophysical field sensors, a filtering plant for chlorophyll concentration, a filtering plant with a Seitz funnel for microorganisms sampling, a Nageotte chamber for counting the phytoplankton content, a Bogorov Counting Chamber for enumerating zooplankton, a centrifugal apparatus to determine chlorophyll content, a geophone, spectral sensor of proton spin echo is proposed. Besides, the proposed system comprises devices for chemiluminescence, chromatographic, ion-selective, spectral and radiometric analysis, a radiation spectrometer, an atomic absorption spectrophotometer, an X-ray fluorometric analyser, TV sensors, infrared sensors, heat sensors, a metrological module, a sidescan sonar, multiple-beam echo sounder, water quality evaluator by TropoSample parameters and bed deposits characteristics, a lidar (a light radar), a penetrometer, methane and hydrogen detection sensors.
EFFECT: enhanced functional capabilities.
2 cl, 11 dwg
SUBSTANCE: invention relates to a method of concentrating salicylic acid from aqueous solution, involving extraction with trioctylamine oxide solution in hexane, deposited on foamed polyurethane tablets in amount of 75-80% of the weight of foamed polyurethane.
EFFECT: invention increases concentration coefficient of salicylic acid.
2 tbl, 4 ex
SUBSTANCE: system for rapid biological monitoring and indication consists of measurement-detection, analytical and signal units. The measurement-detection unit is n apparatus for measuring reactions of aquatic indicator organisms, where n=2, 3, 4, for two or more aquarium in which there are indicator organisms, into which water enters from a distributing aquarium, said water being pumped by a pump from the tested underwater horizon of the water body or from water pipe. Parameters of functional characteristics of the indicator organisms are calculated from signals of measuring apparatus coming into the analytical unit which comprises a computer with software, containing a data base of parameters of the state of functional characteristics of different indicator organisms under normal conditions, configured for constant population and editing. Values of the measured parameters are continuously processed by the computer in real time, separately for each individual indicator organism. Upon deviation of average values from standard values, the signal unit is automatically switched off and a three-step alarm signal is generated - upon deviation from the standard on one parameter, on three parameters and on all parameters for all indicator organisms.
EFFECT: high accuracy and reliability of continuous indication of the quality of water.
3 cl, 7 dwg
FIELD: processing procedures.
SUBSTANCE: invention can be used in analytic chemistry for sorption concentration and successive determination of heavy metals in water solutions. The procedure for production of sorption material consists in impregnation of surface of a cellulose filter with an analytic reagent wherein thio-semi-carbazone of picoline aldehyde is used as such. Impregnation is carried out with conditioning cellulose material in solution of the reagent in ethanol containing 2.5 % of cetyl alcohol with successive extraction and drying in air. Produced cellulose material is applied for sorption-roentgen-fluorescent analytic determination of heavy metals in water solutions. Metals are extracted with cellulose material for roentgen-fluorescent determination at pH 7.5-10.5, preferably, at pH 10.0.
EFFECT: simple and safe procedure for production of sorption cellulose material used for efficient concentration of heavy metals with successive determination of each of them separately and in whole.
4 cl, 2 tbl, 2 ex
SUBSTANCE: in order to realise the method, aromatic amines are extracted from waste water with an emulsion of aqueous solution of an inorganic acid with ionisation constant higher than 10 in an organic solvent.
EFFECT: high degree of extraction of aromatic amines from waste water with minimum consumption of reagents and combining extraction and re-extraction processes at one step.
SUBSTANCE: method of determining crystallisation of heavy isotope types of water during volumetric, uniform cooling of natural water and formation of ice of heavy water involves determining and recording changes in optical properties of water using a laser beam and two photocells. The photocells are placed at different heights and the laser beam and its scattered radiation are picked up. The laser beam is pulsed with pulse duration of up to 1 second and period between pulses of 30-200 seconds. Measurements are taken after lowering temperature of the processed water to +4°C. Before each measurement, the water aeration process is stopped completely or only on the area under the beam for the period of time when bubbles surface.
EFFECT: invention increases quality of water and preserves its salt composition.
SUBSTANCE: nanobacteria are counted in a human nephrolith. A fixed mass is separated from the latter, mechanically powdered and divided into j=5 weight fractions pj. The powder is poured into j=5 sterile cells, water infiltrate at pore size not exceeding 0.05 mcm is added. The concentrations of nanobacteria is set between 102 to 106 cells in 1 ml by varying the water volume Vj or weight fractions pj of a powder mineral mass in each cell with using the formula. It is followed with mixing poured into j measuring cells. A nutrient medium - calves' fetal serum is added in the ratio 1:9. Two electrodes are inserted in each cell, then the measuring cells with the mixture is placed in an autoclave wherein constant temperature within 30°C≤T≤40°C is maintained. A mixture impedance (R) is periodically measured, and a point of measurement time (t) is determined until a mixture impedance slump is observed. A calibration diagram of an impedance variation time (timpj) to the concentration of nanobacteria in an initial sample (timpj) is presented. Thereafter, the above-stated stages of the method are conducted for analysed water as well. The derived impedance time (timpj) values are projected on the calibration diagram on the axis (timpj), then on the axis (lgnj).
EFFECT: invention allows evaluating the water concentration of nanobacteria.
3 dwg, 1 ex
SUBSTANCE: method involves predicting composition of a nonvariant solution, experimental determination of compositions of mixtures on boundaries of the nonvariant region and nonvariant liquid phases with optimum initial mixtures of components, lying in a strictly defined order, from measurements of the physical property of the liquid phase after establishing equilibrium using the "composition-property" functional relationship. Further, for all experimental points lying arbitrarily on all boundaries of the nonvariant region, based on the average ratios of content of the component which is absent in compositions of equilibrium solid phases on corresponding planes or extreme nodes of the nonvariant region of the system to content of water, the composition of the nonvariant liquid phase is calculated and equilibrium solid phases are determined using true coordinates.
EFFECT: obtaining accurate data on phase equilibria in a system without using chemical analysis methods, considerable increase in accuracy mathematical prediction of the composition of the nonvariant solution, avoiding loss of accuracy and reliability of determining composition of the nonvariant solution with increase in the number of components in systems of any type.
4 tbl, 3 dwg, 2 ex
SUBSTANCE: in the method for rapid detection of heavy metals in water, a biosubstrate is mixed with a solution containing heavy metals, wherein the solvent used is ethyl alcohol and water in ratio of 6:1, and the structure index of crystallograms obtained in the presence of salts of heavy metals Cu2+, Fe2+, Zn2+ is determined.
EFFECT: simpler and faster analysis.
SUBSTANCE: system of preparing mineral water of open water bodies for use as therapeutic-preventive means of external application, including system of selecting a sanatorium-and-spa institution, containing successively connected unit of patient's data introduction, unit of formation of data about patients and unit of memory of data about patients, and successively connected unit of introduction of data about sanatorium-and-spa institutions, unit of formation of data about the said institutions, successively connected unit of introduction of data about contraindications, unit of formation of data on contraindications, unit of memory of data about contraindications, connected with its inlets with each of said memory units unit of selection of said institutions, it also has computer control system with buses of its connection with other units, and additionally introduced are successively connected to each other system of analysis of main spa parametres of mineral waters of specified region, system of estimation of their therapeutic properties, system of spa conclusion formatting, system of composing register of water bodies, scheme of water body selection, scheme of selecting procedure of its development, system of selecting technological scheme of elaboration, system of preparation of elaboration organisation, system of preparation for start of operation, system of selection of sanatorium-spa institution being connected with the second outlet of system of register composition, whose second inlet is switched to outlet of system of selecting technological scheme of resource development, it also includes connected to each other system of estimating exploitation resources, system of determining prognosis resources, connected with system of selecting technological scheme of development and scheme of carrying out marketing analysis, connected with scheme of formation of consumer net, connected with system of start of operation, and connected with system of spa conclusion formatting system of preparing specialists, and control system includes automated database in form of spa analysis of mineral waters of local water bodies, therapeutic properties known for such waters and known technologies of developing resources and wells.
EFFECT: method makes it possible to use local natural waters for treatment of population.
FIELD: veterinary science.
SUBSTANCE: method includes the following stages: getting a sample from a poultry's organism, where the sample is selected from the group, including blood, serum, plasma, tissue scrapes, swabs, smears, tissue or separation of bacteria proteins selected from the group Pasteurella trehalosi and/or Mannheimia haemolytica, which are deposited under the registration numbers ATSS No. RTA-3667, ATSS No. RTA-3668, ATSS No. RTA-3669, with the help of gel electrophoresis on polyacrylamide gel and their transfer to a suitable carrier; incubation of the specified sample or the carrier with antibodies specific in respect of bacteria selected from the group Pasteurella trehalosi and/or Mannheimia haemolytica, which are deposited under the registration numbers ATSS No. RTA-3667, ATSS No. RTA-3668, ATSS No. RTA-3669, and detection of availability of antigen-antibody complex.
EFFECT: group of inventions has high diagnostic accuracy.
3 cl, 2 tbl, 6 ex
SUBSTANCE: without grinding, a sample of freshly collected pathological material is put into an accumulation medium - guanosine monophosphate broth with addition of 1% glucose and then cultured in a temperature-controlled cabinet at 37°C for 24 hours. Seeding is then carried out on a Petri dish with agar medium with addition of 5% erythrocytes and on enterococcus agar. The inoculations are kept in a temperature-controlled cabinet for 24-48 hours at 37°C. Samples which failed to grow in aerobic conditions are further inoculated on a Petri dish with agar medium with addition of 5% erythrocytes and then put into an anaerobic jar for 24 hours at 37°C. The inoculations are examined under a microscope and microorganisms are identified from the results using traditional methods.
EFFECT: higher isolation rate of enterococcus in anaerobic conditions at 31,2-36% and possibility of detecting presence of microorganisms in clinical material which do not grow in aerobic conditions.
FIELD: chemistry; biochemistry.
SUBSTANCE: microorganism is conjugated with nanoparticles of a magnetic substance in the analysed medium with subsequent concentration of the conjugates and determination of presence and concentration of microorganisms using a diagnostic mark. Nanoparticles of transition metals or their compounds simultaneously serve as the magnetic substance and the diagnostic mark. Before concentration of labelled conjugates from the analysed medium, nanoparticles which are not bonded to microorganisms are extracted. The labelled conjugates are concentrated by forming an immunocomplex on a solid chemically inert carrier: antibody microorganism labelled with a magnetic mark, with subsequent extraction of the immunocomplex from the medium on the carrier. Presence and concentration of microorganisms is determined from a signal generated by transition metal ions obtained through chemical decomposition of the immonocomplex.
EFFECT: method is aimed at increasing sensitivity and reliability of analysis, and widening the range of determined.
6 cl, 9 dwg, 8 ex
SUBSTANCE: genetically modified cell of yeast is produced either by introduction of an alien gene, or by variation in expression of its own gene. To compensate effect of these genes in cell, expression of certain genes is varied in ascending (up-regulation) or descending (down-regulation) manner. Using micromatrices (microarrays) of DNA, they identify compensatory expressing genes. Compensatory genes are affected, which results in reduction or cessation of expression of up-regulated genes or improved expression of down-regulated genes. As a result, a phenotype occurs in cell, which expresses as inhibitor of its growth.
EFFECT: invention makes it possible to produce organism for screening of biologically active substances, which affect genes, expression variation of which does not show in phenotype manner.
9 cl, 1 dwg, 4 tbl, 2 ex
FIELD: medicine; veterinary science.
SUBSTANCE: bacteriophage preparation as contains suspension of strains Phagum Salmonella typhimurium No.5 "ТЗ-ДЕП" of concentration 3.6×108 PFU/cm3 and Phagum Salmonella typhimurium No.8 "МЕ - ДЕП" of concentration 1.0×107 PFU/cm3 in equal relation in culture medium preserved in quinosol in the component ratio as follows (per 1 l of the culture medium): suspension of strain particles Phagum Salmonella typhimurium No.5 "ТЗ-ДЕП" - 3.6x108 PFU/cm3, suspension of strain particles Phagum Salmonella typhimurium No.8 "МЕ - ДЕП" - 1.0x107 PFU/cm3, quinosol - in end concentration 0.01%. Pigeons are fed with the preparation in a dose 0.25-0.5 ml every 48-72 h. The preparation may be added to potable water at 5-10 ml per 1 kg of body weight for one feeding. Before feeding, birds are kept without water for three hours.
EFFECT: preparation and related application method are safe, allow for effective treatment of salmonellosis infection, protect against said disease from first days of life, prevent postvaccinal complications and improve safety of livestock population.
4 cl, 2 tbl, 2 ex
SUBSTANCE: invention relates to the field of medicine. Prognostic method is based, on one hand, on integral indices of macroorganism condition, and on other hand, on microorganism pathogenicity factors. In soft tissue phlegmonic patients, presence and intensity of system inflammatory response syndrome is evaluated. From biomaterial in the nidus of surgical infection, the pure pathogen culture is isolated, its species is identified, its virulent and persistent characteristics are determined, and risk of local purulent complication development is calculated by formula R=-5.699x1-3.828x2-6.210x3-2.525x4-5.699x5-2.495x6-3.066x7-3.703x8; if R≥0, the prognosis is favourable, if R<0, local purulent complication development is prognosticated. In case of unfavourable prognosis, pathogen elimination period is determined by formula y=0.638x+1.06, where y is pathogen elimination period, x is pathogen anticomplementary activity level.
EFFECT: providing objectivity and reproducibility; allowing timely risk evaluation of local purulent complication development.
1 dwg, 2 ex
FIELD: organic chemistry, microbiology, medicine, oncology.
SUBSTANCE: invention relates to novel class of antitumor compounds found in isolation from new marine microorganisms of genus Actinomadura sp., strain PO13-046, namely, to compound denoted as IB-00208: and relates to class of compounds of the formula (I) or (II) , wherein each substitute R1 is similar or different and can represent hydrogen atom, acyl, alkyl, alkenyl, aryl, benzyl, alkaline metal and/or monosaccharide, disaccharide, trisaccharide or their derivative; R2 and R3 can represent hydrogen atom or alkyl. These compounds show antitumor activity.
EFFECT: valuable medicinal properties of compounds.
8 cl, 3 tbl, 9 dwg, 11 ex
FIELD: medicine, in particular, laboratory-based methods for diagnosing tuberculosis.
SUBSTANCE: into diagnostic material, after processing with detergent - chlorhexidine gluconate prior to seeding in dense nourishing substances additionally injected is freshly prepared water solution of hydro-gel of methyl-silicon acid in ratio 2:1, shaken, centrifuged, to precipitation 1 ml of special substance is added, product is taken to dense nourishing substance.
EFFECT: method accelerates growth of tuberculosis mycobacterium and accumulation of bacterial mass, which is important for differential diagnosis of tuberculosis.
FIELD: biotechnology, in particular methods for biomass flocculation from suspending media.
SUBSTANCE: claimed method includes addition the first cationic polymer material into suspending medium, wherein said material has intrinsic viscosity not more than 2 dl/g, and simultaneous or subsequent addition of the second cationic or substantially non-ionic polymer material into suspending medium, wherein said material has intrinsic viscosity of at least 4 dl/g. Said dosage provides optimum characteristics for biomass flocculation. Method of present invention makes it possible to obtain flakes with increased endurance, and as a result to apply various isolation methods including those based on mechanical flake agitation.
EFFECT: improved method for biomass flocculation from suspending media.
15 cl, 4 tbl, 6 ex
FIELD: biotechnology, veterinary science.
SUBSTANCE: invention relates to the strain that is deposited in the Collection of strain-pathogens of diseases in fur animals in the Museum of bacterial and viral structures of the Science-Research institute of fur farming and rabbit farming (NIIPZK) named for V. A. Afanasiev and has the registration number 71. The strains relates to streptococci of the group B. The strain is cultures in Todd-Hewite beef-extract broth, Pike medium at 37°C for 18-20 h. After control of the grown culture for virulence the separated biomass is inactivated by addition of 0.5% formaldehyde followed by addition of aluminum hydroxide as an adjuvant and packaging. Invention provides preparing the effective vaccine against streptococcosis in fur animals based on this strain.
EFFECT: valuable properties of strain.
FIELD: microbiology, pharmacy.
SUBSTANCE: invention relates to a method for isolating biomass of microorganisms from cultural fluid and can be used in manufacturing preparative formulations of biopreparations. Method involves addition of high-molecular reagent "Givpan" as a flocculant representing a product of hydrolysis of polyacrylonitrile copolymer of acrylonitrile with methacrylate. "Givpan" is added in the amount 0.1-0.8 wt.-% of the cultural fluid volume. Applying the proposed method allows retaining activity of microorganisms to be isolated that are antagonists of fungal phytopathogens and amount of viable cells.
EFFECT: improved isolating method.
3 tbl, 3 ex