Automatic device to colour smears on microscope slide

FIELD: machine building.

SUBSTANCE: proposed device comprises sealed work chamber, stand, baths, pans, work table accommodating stations with bath positioning devices, pans, movable stand drive equipped with gripper, lifting mechanism and drive, and control unit. Automatic device incorporates also sealed work chamber with sealing side, drying chamber, driven rotation mechanism, stand with "П"-shape handle, stand base is rounded and skewed at 20÷70°. Stand sidewall inner surface supports separators made up of a comb with ledge thickness of 1÷4 and spacing width of 1.1÷2.0 of microscope slide thickness. Movable stand drive incorporates gripper made up of rectangular-section bucket. Note here that gripper moves along circular arc within 0÷40°. Baths and pans are provided with eyelets. Stations are equipped with positioning devices made up of cone pairs with truncated apex and cylindrical location flange, its diameter making 0.80÷0.95 of eyelet diameter. Aforesaid "П"-shape handle has length making 1.1÷1.2 of relation between gripping height and drive arm lifting angle cosine. Bath wall skew makes 5÷20°, wall facing stand drive.

EFFECT: higher efficiency and reliability, ruled out fouling.

1 tbl, 4 dwg

 

The invention relates to medicine, namely to devices for painting Biologicals (smear) on slides.

In Hematology, Cytology, Microbiology widely used to prepare microscopic preparations for the study, it is necessary, in particular, to determine the leukocyte blood, diagnosis of cancer, detection of infection by microorganisms, etc.

Pre-analytical phase includes the taking of a biological sample, for example, directly from the body of the patient, applying a smear on a glass slide, pre-processing, fixation and coloration special process fluids, for example a set of process fluids for gram staining. Staining of products allows us to identify the various structural elements of the assay and therefore effectively conduct microscopic examination and diagnosis.

Known apparatus for staining smears on slides (prototype, U.S. patent No. 5573727, 1996), containing

- sealed chamber with lid;

- tubs, trays;

- a tripod with a U-shaped handle and base, with curves and bevels, and on the inner surface of the side walls of the tripod by means of fixing elements are mounted separators in the form of combs;

- the control unit and is of antros;

- desktop hosting station;

- movable carrier tripods with capture;

- lifting mechanism, driven by a lifting drive.

A disadvantage of the known device: move the capture of the rolling carrier tripods limited. The grip can change its position only along the vertical axis of the rolling carrier tripods that prevents complete swelling of the process liquid from the surface of the slides.

In case of incomplete swelling process fluid from the surface slides on the surface of smear may appear spots, crystal allocation, preventing quality smear; due to the transfer of the process liquid on the surface of the slides, and the stroke volume of the bath in the tub is the contamination of the process fluid.

When applying the known device for staining smears on slides sufficiently swelling the process liquid from the surface of the subject can lead to marriage.

The technical result that corresponds to the claimed machine for paint smears on slides, is that you effectively uniform change process fluids on the surface of smear due to modes rinsing, activation, full, in the support, efficient and uniform removal of the fluid from the surface of the glass and of the volume of the stroke due to the modes of extracting, shaking and drying; when this is not possible pollution of the environment, because all of the painting process takes place in a closed chamber.

The technical result is achieved by a machine for coloring smears on slides has a sealed working chamber with a side sealing, cover, tripod with a U-shaped handle, a movable carrier tripods is an arrow with stepper drives and seizure made in the form of a bucket of rectangular cross-section, the grip is arranged to move in an arc in the range 0÷40°, trays and tubs have positioning means in the form of pairs of eyelets; station, with means for positioning have a means of positioning in the form of pairs of truncated cones with a top and a cylindrical mounting band, the diameter of which is equal to 0.80÷0,95 the diameter of the lugs, trays and tubs made with slanted side walls, U-shaped arm tripod with bevels and curves has a length equal to 1.1÷1.2 relationship of the height of the safety device to the cosine of the angle of the lifting of the seizure in the engaged position of the pair grip handle tripod, baths are beveled wall facing the rolling carrier racks, angled 5÷20°.

the alignment properties of the combination of features of the known device and the inventive shows that

- the claimed machine equipped with a drying station, which is absent in the known device;

in contrast to the known, the claimed movable carrier tripods has a rotation mechanism with a drive rotation;

- capture of the rolling carrier tripods in the known device can move only vertically, and in the inventive device capture, coupled with a drive tilt, can move in an arc in the range 0÷40°;

the capture is made in the form of a bucket of rectangular cross section;

- bevel the base of the tripod angled 20÷70°;

in contrast to the known apparatus, in the inventive device movable carrier tripods can operate in modes shaking, activation, slow recovery due to the presence drives with stepper motor and program management;

- in the inventive device tripods equipped with a U-shaped handle with bevels and curves, having a length equal to 1.1÷1.2 ratio of capture to the cosine of the angle of elevation of the boom rolling carrier tripods and tripod base made with curves and slanted at an angle of 20÷70°;

- in the inventive device, the bath is made with slanted walls facing the rolling carrier tripods angle 5÷20°;

- in the inventive device, the grip has a shape and dimensions that provide a wedging it P-clicks the Noah arm of the tripod in the engaged position and the free position of the handle grip in the lower position;

in contrast to the known, in the inventive device, the motor and drive mounted on the same shaft, which ensures high reliability of the lifting mechanism and rotation of the rolling carrier tripods;

- in the inventive device stations have positioning means in the form of pairs of cones, which greatly facilitates the cleaning of the working chamber;

- in the inventive device trays and baths have positioning means in the form of pairs of eyes.

Therefore, the inventive device is characterized by distinctive features contributing to inventive step, the technical solutions.

The structure of the device and its operation are explained in Table 1 Protocol process program gram staining and graphical materials figure 1-4,

where 1 is the working chamber; 2 - side sealing; 3 - cover; 4 - Desk; 5 - bath; 6 - pallet; 7 - pairs of cones; 8 - cylindrical adjusting belt; 9 - lugs; 10 - movable carrier tripods; 11 - capture; 12 - bolt; 13 - control unit, 14 - touch screen; 15 - tripod; 16 - slides; 17 - arm; 18 - station color; 19 - wash station; 20 - station drying; 21 - station Parking; 22 - occupancy sensors; 23 - separator; 24 - ledges; 25 - grooves under the glass.

Figure 1. General view of the machine for paint smears on slides.

Figure 2. Accommodation baths, trays is and the desktop.

Figure 3. Working position of the moving vector of tripods and tripod-up of slides,

where b=(1,1÷1,2)h/cosα,

b - arm length of the tripod vertically;

h - height;

α is the angle of the lifting of the seizure.

(a) the initial position vector of the tripod and tripod with slides

b) the engaged position the handle of the tripod-up of slides in engagement with capture;

in the transport position vector of the tripod and tripod-up of slides.

Figure 4. Tripod-up of slides.

Machine for paint smears on slides consists of a rectangular working chamber 1 with a side seal 2 having a transparent wall, with the cover 3. On the desktop 4 posted by stations staining (figure 2, p), flushing (figure 2, p.19), drying (figure 2, paragraph 20) and Parking lots (figure 2, item 21), equipped with means of positioning the bath 5 and the pallet 6 in the form of pairs of cones 7. Cones 7 are truncated top and a cylindrical adjusting belt 8, the diameter of which is equal to 0.80÷0.95 diameter of eyes. The wall of the bath 5, facing the rolling carrier tripods, is slanted at an angle 5÷20°. The pallet 6 is rounded and slanted at an angle of 20÷70°. Tub 5 and the pallet 6, in turn, equipped with lugs 9 for positioning at the stations by means of fastening to the cones 7. Movable carrier rack 10 has a grip 11, the mechanism by which Yama, driven by a lifting drive mechanism rotating with the rotation drive (not shown). Running programs paint smears on slides using power management and control 13. Touch screen 14 can monitor the movement of the tripod 15-up of slides 16 from station to station. The tripod 15 provided with a U-shaped handle 17 with bevels and curves. Capture 10 of the rolling carrier tripods 9 has a shape and dimensions that provide a wedging therein the U-shaped arm 17 when engaged in the transport position and the released state in the lower position.

The separators 23 are mounted on the inner surface of the side walls of the tripod 15 by means of fixing elements in the form of a comb with thick lugs 24, is equal to 1÷4, and the width of the grooves 25, equal to 1.1÷2,0 thickness of the slide, which allows for the installation of the slide into the separator vertically without tilting.

Automatic execution of programmed methods movable carrier tripods 9 includes:

1) jamming of the arm 17 of the tripod 15 in the capture of 11 (figb);

2) the rise of the tripod 15-up of slides 16 in an arc with tilt;

3) transfer tripod from station to station (pigv) and tripod mounting in the bath or directly on the pallet (figa).

4) move the tripod in the process is hidcote and outside in the given modes (rinsing, extract, activation, dipping, slow rise, shaking).

Fixing, dyeing, rinsing and drying are carried out in a closed working chamber at stations dyeing, washing, drying (station - part of the desktop, provided with means for positioning baths, trays, racks-up of slides and intended for occupancy) for a given program, moving slides occurs inside a closed working chamber with the aid of the moveable carrier racks, to ensure effective and uniform contact of the biological preparation with process fluids used a different sequence of movement of the tripod with glass in the fluid and the outside (the lower the glass into the process liquid, rinsing the glass in the process liquid (1-3 reciprocating motion up and down immediately after lowering the glass in the process, liquid); activation (periodic reciprocating motion in the fluid up and down, followed by a pause, period - 1÷60 sec; dipping the glass into the process liquid for a short period of time 0,2÷2,0 sec, followed by a short excerpt out of process fluid within 0,2÷10.0 sec with an accuracy of at least ±0,006 sec, the shutter glasses in the process liquid within the how many minutes or tens of minutes: 0,5÷60,0 min, slow removing glass from the process liquid at a rate not exceeding 10÷12 mm/sec for the efficient removal of the fluid from the glass and from the space between the glass, shaking the tripod with glass to effectively remove residual process liquid from the glass and from the space between the panes, drying glasses in a stream of warm air between the individual operations to remove liquid from the surface of the glass and of the volume of the stroke, the final drying after conducting all operations in the process liquid. To control the drying process is provided for automatic temperature regulation; and to control the availability of tubs and trays at stations dyeing, washing, drying installed occupancy sensors.

The operation of the machine is as follows.

The researcher deals smear on a glass slide 16 with a spatula or other means, for example by means of centrifuge, dry smear within 1÷5 minutes at a temperature of 15÷35°C and sets the glass slide in the tripod 15. Then the researcher through the menus on the touch screen programming the operation of the machine for paint smears on slides (figure 1) in accordance with the selected method of staining, installs inside of the working chamber 1 bath 5 with process fluids and the pallet 6 in the manner soo is relevant to the programme of work of the machine, and stator 15-up of slides 16 on the pallet station Parking 21. After filling tripod 15-up of slides, the operator places the tripod on the tray, closes the lid 3 of the machine for coloring smears (figure 1), the machine detects raw tripod and starts a scheduled process program using at least two process fluids for painting and washing of the stroke.

From the station Parking 21 movable carrier tripods 9 moves the tripod 15-up of slides 16 to the station colouring 18, where the shutter slides slides in a solution of latch-dye within the specified time program.

For the uniform implementation of technological parameters, flush with the surface of the glass liquid from the previous bath and ensure contact of the stroke with the process fluid tripod when immersed in the process liquid is subjected to rinsing (reciprocating motion up and down 1÷5 times).

To activate the process of staining smears on slides movable carrier 10 tripods periodically raises and lowers the tripod.

To provide treatment in a mixture of different process fluids used single or repeated sequential quick dipping tripod first one, the technology will stifle the liquid, and then in another; in the subsequent exposure of the films of different fluids on the surface of smear shuffled through diffusion, and treatment of the stroke occurs in the mixture of process fluids. This enables a number of high-speed techniques that are not possible in manual mode and/or reduce the number of baths with the process fluid.

To remove the fluid from the glass and tripod due to the surface tension of the fluid is slow to remove the glass and tripod from the process liquid.

To remove residual process liquid from the glass and tripod is shaking the glass and a tripod.

To remove the fluid from the glass and from the stroke is applied intermediate (between other technological operations) or final drying.

At the end of the process is movable carrier tripods 9 moves the tripod 15-up of slides on the original pallet in the station Parking and alerts the technician about the end of the process.

Painting and washing smears on slides may take several baths in a variety of process liquids. Removal of process fluids from the surface of treated glass slides helps slow the rise of the tripod in an arc with tilt tripod and the address of the rolling carrier racks 10 in the mode of shaking.

The final operation - transportation of the tripod-up of slides into the station Parking 21.

You can run multiple programs at the same time.

Movable carrier rack 10 is focused on a signal from the sensors 22, which controls the availability of the stations baths with process fluids. The presence in the bath of the tripod-up of slides stored in the memory of the rolling carrier tripods.

All stations, except station drying, combined, i.e. can be used as Parking when you install them in pallets, and can be used as a process when you install them in the tubs with the process fluid.

In the case of the combined use of all stations under a bath with process fluids tripod for processing is placed in the drying station.

Machine for paint smears on slides made in shops LLC "EMKO". There are technological documentation of repeated playback device.

Therefore, the claimed device meet the criterion of "industrial applicability".

Machine for paint smears on slides, including a sealed working chamber, tripod, tubs, trays, Desk, hosting station with means positioning is of baths, pallets movable carrier tripods equipped with a gripper, a lifting mechanism with a lifting drive, power management and control, characterized in that the machine has a sealed working chamber with a side sealing station drying, the rotation mechanism with a rotational drive, a tripod with a U-shaped handle, the base of which is made with curves and slanted at an angle of 20÷70°on the inner surface of the side walls of the tripod by means of fixing elements are mounted separators in the form of a comb with the thickness of the protrusions is equal to 1÷4 and width of grooves equal to 1,1÷2,0 thickness of the slide; a movable carrier tripods has a grip in the form of a bucket of rectangular cross-section, and a grip arranged to move in an arc in the range 0÷40°, tubs and trays are equipped with eyelets, stations have a means of positioning in the form of pairs of truncated cones with a top and a cylindrical mounting band, the diameter of which is equal to 0.80÷0.95 diameter of the eyelets, and the U-shaped handle with bevels and curves has a length equal to 1.1÷1.2 ratio of capture to the cosine of the angle of elevation of the boom rolling carrier tripods; baths are beveled wall facing the rolling carrier racks, angled 5÷20°.



 

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