Method of sublimation dehydration of high-disperse biologically active materials

FIELD: medicine, pharmaceutics.

SUBSTANCE: invention refers to pharmaceutical industry and represents a method of sublimation dehydration of high-disperse biologically active materials found in a microdrop state characterised by the fact that the microdrop powder is frozen at temperature -35°C to -45°C for 1.5 to 8 h, and then dehydrated.

EFFECT: invention provides reduced duration of the dehydration process of biologically active materials.

3 ex

 

The invention relates to medicine and the pharmaceutical industry and relates to a method of obtaining dry biologically active materials dehydration by sublimation.

A method of obtaining dry preparation on the basis of live lactobacilli for the treatment and prevention of dysbacteriosis of various etiologies in humans and animals, which provides for obtaining biomass of cells deep cultivation in liquid nutrient medium, its freezing and freeze-dehydration while before freezing to biomass lactobacilli add poliglyukin to a final concentration of 1.5% (wt./vol.), filling microbial suspension in vials exercise at a constant work mixing device of the apparatus (the speed of rotation of the stirrer - (0.3 to 0.5).min-1), and freeze carried out at a speed of 2°C/ min-1(RU, patent 2223775 C1, AC 35/74, C12N 1/20, 20.02.2004).

A known method of obtaining a bacterial preparation comprising the filling of the drug in the container, its freezing, sublimation and final drying on the shelves of the freeze installation, with the freezing of the drug is carried out at an angle to the axis of the container relative to the vertical at an angle between 45 and 75 degrees, and the process of sublimation is carried out at the heated shelves with a rate of 10-15°C per hour to a temperature of 30-35°C (RU, patent 2322161 C1, A23L 3/44, C12N 1/04, 20.04.2008).

The main defects in the rigid-known methods of freeze-drying products is their long duration.

The basis of the claimed invention is the task of reducing the duration of dehydration process of biologically active materials.

The problem is solved by the fact that the liquid phase is frozen and dehydrated from drip condition, stable dry finely dispersed hydrophobic disconnector with nano particles.

As a result of our research for the first time shows that the benefit of the dehydration of liquids containing biologically active substances from drip condition, stable dry finely dispersed hydrophobic disconnector with nano particles, is that this condition generates the expanded surface of the liquid in the powder component according to our data 0,04-0,09 m21 cm3powder that provides a large area of moisture evaporation, and consequently the short duration of the removal of the weight of free moisture in the surrounding space, as the process is carried out from the total volume of dried powder. In addition, the process of freezing of the material prior to sublimation occurs much faster, as is done in particles of very small volumes, because the maximum size of stable droplets does not exceed, as a rule, 30 μm, and is dried particles even smaller is size, educated dissolved in the droplets of the substances remaining after evaporation. Specified significantly reduces the duration of all stages of freeze drying: freeze the liquid phase, sublimation of moisture and dried.

According to the invention the reduction in the duration of dehydration process of biologically active materials is ensured by the fact that the liquid phase is frozen and dehydrated from drip condition, stable dry finely dispersed hydrophobic disconnector with nano particles.

The inventive method of freeze dehydration high dispersion of the biologically active material is new and are not described in literature.

The technical result of the claimed invention is the reduction in the duration of dehydration process of biologically active materials.

The invention is illustrated in the following examples, showing the reduction in the duration of dehydration process of biologically active materials during implementation of the method.

The content in the preparations of viable aerobic microorganisms Serratia marcescens was determined by the method of Pasteur-Koch on solid nutrient media. The content of viable anaerobic microorganisms Bifidobacterium bifidum was determined in a liquid nutrient medium by the method of limits is lnyh dilutions. Biological activity of antibodies preparations characterized protivoallergennoy activity (in the credits TPHA) [FS 42-3347-97].

Example 1. The object of dehydration were prepared by mixing a concentrated suspension of the microorganism Serratia marcescens pieces VKM-851 with lactase protective environment in the ratio of 2:1 and translated it into drip condition in the electromagnetic dispersant. Drip powder indicator of culture with the liquid phase in drip condition, stable dry finely dispersed hydrophobic disconnector with nanosized particles with concentration of viable microorganisms 202×109CFU/g were initially frozen at a temperature of minus 35°C within 1.5 h on a shelf of sublimator, and then dried by sublimation apparatus TG-5 (Hochvacuum, Germany), using modal parameters, described in the work [the Way to get the culture test / Davydkin YOU, Davydkin V.Y., Alibekov K.B., Davydkin P., Egorov OP / Advanced manufacturing experience in the medical industry that are recommended for implementation. - 1991. - VIP - 12. - p.6-10].

The biological activity of the dry drug culture test to check air filters amounted to 328×109CFU/g, and the total duration of the process was reduced to 12 h, 6 h in less than with the known method.

Example 2. The object of both is varovanie were prepared by mixing suspensions of microorganisms Bifidobacterium bifidum pieces 1C with Saharsa-breast protective environment in the ratio of 2:1 and translated it into drip condition in the disk disperser. Drip powder probiotic preparation with the liquid phase in drip condition, stable dry finely dispersed hydrophobic disconnector with nanosized particles with concentration of viable microorganisms of 2.1×109CFU/g were initially frozen at a temperature of minus 35°C for 8 hours, and then dried by sublimation apparatus LZ-9.2 (Frigera, Czech Republic)using modal parameters, described in the work [Aleneva BTW, the Effectiveness of different ways of storing and dewatering of biomass in the production of tableted bifidumbakterin: Dis. Kida. Biol. Sciences. M., 1984. - s.89-94].

The biological activity of dried probiotic preparation was 3.0×109CFU/g, and the total duration of the process was reduced to 20 h, 24 h less than with the known method.

Example 3. The object of dehydration were prepared by mixing a solution of immunoglobulins IgG, IgA, IgM with glycine (2%) as a protective environment and translated it into drip condition in the disk disperser. Drip powder immunobiological preparation of liquid phase in drip condition, stable dry finely dispersed hydrophobic disconnector with nano-sized, the mi of the particles, with protivoallergennoy activity 1:640 in the credits TPHA were initially frozen at minus 45°C for 6 h, and then was dried by sublimation apparatus LZ-9.2 (Frigera, Czech Republic), using "soft" mode parameters described in [Testing process of freeze-drying complex immunoglobulin preparation / Davydkin V.Y., Gavrin A.G., Aleshkin V.A., etc. // Problems of infectious diseases. - M., 2000. - Part 2. - p.61-66].

Articleonline active dry immunoglobulin preparation was 1:1280, and the total duration of the process was reduced to 14 h, 9 h in less than with the known method.

The method of freeze dehydration highly dispersed biologically active materials in the drip condition, characterized by the fact that drip powder freeze at temperatures from -35°C to -45°C for 1.5 to 8 h, and then dehydrated.



 

Same patents:

FIELD: medicine, pharmaceutics.

SUBSTANCE: invention relates to pharmaceutical industry and represents a method for producing fine-grained biologically active materials containing active substances, characterised by the fact that the liquid with biologically active substances is dispersed to the microdrop state in a layer of dry fine-grained inert hydrophobic aerosil in the proportion 10:1.5 to 10:6, to form thereby liquid microdrops surrounded by hydrophobic aerosil particles and powdered which, if necessary, are dried by a technologically acceptable method.

EFFECT: invention provides increasing dispersion of biologically active materials.

11 ex, 2 dwg

FIELD: medicine, pharmaceutics.

SUBSTANCE: invention refers to pharmaceutical industry and represents a method of combination dehydratation of high-disperse biologically active materials containing active substances in a liquid phase, implemented in stages and differing by the fact that the liquid phase with the active substance in a microdrop state stabilised by a dry high-disperse hydrophobic disconnector in ratio 1:3-1:22, is dehydrated initially at atmospheric pressure and then by mixing with a high-absorbency sorbent with residual moisture less than 1% and dried up if needed.

EFFECT: invention provides higher activity of the active substances.

9 ex, 1 tbl

FIELD: biotechnology, microbiology.

SUBSTANCE: invention relates to a method for preparing preparations used in prophylaxis and treatment of dysbacteriosis in humans and agricultural animals. Method involves preparing liquid biomass by mixing native culture of lactobacilli of the strain Lactobacillus plantarum 8P-A3 grown under condition of submerged culturing with the protein-carbohydrate complex in tissue homogenizer device PT1. Contact dehydration of prepared liquid biomass of lactobacilli is carried out using the moisture capable ion-exchange resin KB-4P-2 with particles size from 1 to 800 mcm treated preliminary with the depleted mixture of anhydrous lactose and hydrophobic aerosil. Invention provides enhancing stability of lactobacilli as component of the dry probiotic preparation and in its preparing, storage and using and to diminish time consumptions in carrying out the dehydrating process of lactobacilli biomass.

EFFECT: improved preparing method.

3 cl, 6 tbl

The invention relates to vacuum freeze-drying heat-sensitive products and can be used in pharmaceutical, microbiological, food industry, and also at the enterprises of agro-industrial complex

The invention relates to vacuum freeze-drying heat-sensitive products and can be used in pharmaceutical, microbiological, food industry, and also at the enterprises of agro-industrial complex

The invention relates to the field of restoration of Museum exhibits and can be used to recover products from organic materials, such as books, clothing, stuffed animals, furniture, etc

The invention relates to the field of biotechnology, particularly to the drying of biologically active materials, and can be used in medicine and veterinary medicine for dry and immunobiological medicinal products in food industry and agriculture
The invention relates to the drying of biological material without the application of heat through contact with the sorbent bodies by mixing sorbing substances, namely, by the way, based on the contact dehydration of biological suspensions by mixing with solid sorbent
The invention relates to the technique of freeze-drying and can be used in microbiological and other industries for drying of cultures of microorganisms
The invention relates to the production technology of drugs, namely the production of dry microbial agents

FIELD: medicine, pharmaceutics.

SUBSTANCE: invention relates to pharmaceutical industry and represents a method for producing fine-grained biologically active materials containing active substances, characterised by the fact that the liquid with biologically active substances is dispersed to the microdrop state in a layer of dry fine-grained inert hydrophobic aerosil in the proportion 10:1.5 to 10:6, to form thereby liquid microdrops surrounded by hydrophobic aerosil particles and powdered which, if necessary, are dried by a technologically acceptable method.

EFFECT: invention provides increasing dispersion of biologically active materials.

11 ex, 2 dwg

FIELD: medicine.

SUBSTANCE: invention refers to a method for making slow-release microspheres containing a biodegradable polymer as a carrier and a drug, and to the drug-loaded microspheres made by the specified method. The method provides spraying in the dry chamber of a solution, suspension or emulsion containing the biodegradable polymer, a drug and a solvent, and air-drying of the solvent to prepare the spray-dried microspheres. The prepared microspheres are dispersed in an aqueous solution containing polyvinyl alcohol to remove a residual solvent and to improve the dispersibility of the microspheres, and the dried microspheres are removed from the aqueous solution.

EFFECT: method allows to make microspheres with high effective drug encapsulation without residual toxic solvent and the improved syringe needle permeability.

11 cl, 3 dwg, 5 tbl, 16 ex

FIELD: medicine.

SUBSTANCE: invention refers to chemical-pharmaceutical industry, and concerns an oral drug delivery system including biliquid foam containing continuous hydrophilic phase 1 to 20 wt %, pharmaceutically acceptable oil 70 to 98 wt % producing discontinuous phase. A slightly water-soluble drug 0.1 to 20 wt % is dissolved or dispersed in said pharmaceutically acceptable oil. The drug delivery system also contains biliquid foam with included surface-active substance 0.5 to 10 wt % to produce stable biliquid foam with all the amounts specified in percentage of total composition.

EFFECT: drug delivery system ensures high bioavailability of slightly water-soluble oral drugs.

22 cl, 16 ex, 1 tbl

FIELD: medicine.

SUBSTANCE: agent for prevention and alcoholism treatment contains admixture of amidocyanogen and polylactide in the ratio (wt) from 10:90 till 40:60. The method of prevention and alcoholism treatment consists that to the patient, as a rule, enter intramusculary a solution containing an admixture amidocyanogen and polylactide in a single dose of 0.5-1.5 g once a month.

EFFECT: appreciable effect of delay and slow liberation of the operating beginning.

4 cl, 3 dwg, 2 tbl, 3 ex

FIELD: medicine; ophthalmology.

SUBSTANCE: invention is characterised by compositions and methods of application of such compositions applicable for injection in posterior ocular segment of people and animals. These compositions include particles containing corticosteroid component of water-soluble factor less than 10 mg/ml at 25°C presented in therapeutically effective amount, thickener and aqueous carrier. Viscosity of compositions is at least about 10 centipoises or about 100 centipoises at shear rate 0.1 c-1. Preferable version of invention implies that viscosity is within the range 140000 centipoises to 300000 centipoises. Particles in compositions mainly remain suspended during continuous period of time.

EFFECT: invention provides stability of composition d during continuous period of time without resuspending.

57 cl, 9 ex

FIELD: pharmaceutics.

SUBSTANCE: the present innovation deals with peroral liquid compositions which could be designed into gelatinous capsules. The suggested pharmaceutical composition includes a pharmaceutically active agent, a solubilizing agent and, not obligatory, a surface-active substance and a plastifying agent. The pharmaceutically active agent has got, at least, one acidic fragment, preferrably, that of carbonic acid being chosen out of the group of non steroid antiphlogistic preparations being acid-soluble at acid : dissolved substance ratio being from 3:1 to 10000:1. New compositions provide increased rates and degrees of absorption of pharmaceutically active agent and minimize side effects caused by such active substances.

EFFECT: higher efficiency of application.

42 cl, 39 ex

The invention relates to the field of molecular biology

FIELD: medicine, pharmaceutics.

SUBSTANCE: invention relates to pharmaceutical industry and represents a method for producing fine-grained biologically active materials containing active substances, characterised by the fact that the liquid with biologically active substances is dispersed to the microdrop state in a layer of dry fine-grained inert hydrophobic aerosil in the proportion 10:1.5 to 10:6, to form thereby liquid microdrops surrounded by hydrophobic aerosil particles and powdered which, if necessary, are dried by a technologically acceptable method.

EFFECT: invention provides increasing dispersion of biologically active materials.

11 ex, 2 dwg

Up!