Method of treating periodontitis
SUBSTANCE: invention relates to medicine, namely surgical stomatology and can be used for treating paradontium. Periodontal osteogenesis and neogenesis are ensured by removing pathologically changed structures and introducing the mesenchymal stem cells suspended in a carrier into the defect cavity. As the stem cells, the mesenchymal cord and placental stem cells received after easy delivery are used. The cells 5-10·106 are injected in 1-2 ml of patient's blood plasma.
EFFECT: method provides more effective replacement of the manifested periodontal defect associated with paradontium, reduced length of treatment with no side effects.
The invention relates to medicine and can be used in dental surgery in the treatment of diseases periodontitis.
Periodontal disease is spreading among the population of the 4-th place in the list of non-communicable diseases, and the age of the patients does not play a role: for 30 years the disease register 50% of the population.
In the development of periodontitis involved exogenous and endogenous factors such as the composition and properties of saliva, microorganisms, Tartar formation, etc. of the Immune and hormonal factors are key in triggering the mechanism of development of severe forms of inflammatory-destructive periodontal disease. Periodontitis is characterized by the fact that in patients affected all parts of the periodontium with bone resorption of the alveolar ridge, resulting in the death of the restraint apparatus of teeth and loss of teeth. By treatment of periodontitis, it is necessary to accelerate the processes neoangel and osteogenesis and repair all tissues affected by disease.
Known way to restore periodontal tissues in the defect with osteoplastic material "Cerasorb", with platelet enriched plasma (Parodontologie, 2004, №3(32). The disadvantage of this method is that the osteoconductive material is saturated with platelet plasma. Because of the availability of the only elements of the blood, regeneration of periodontal tissues is slow.
Known method periodontal treatment using collagen sponge "collegit" and postnatal human fibroblasts directly in the field of periodontal defect (Parodontologie, 2004, №3(32)), material used - collagen-chitosan gel. The disadvantage of this method is the use of postnatal fibroblasts and the use of multicomponent systems in the gel, and the manufacturing time of the gel from 1 to 3 days.
Modern and actively developed a method for the treatment of periodontal defects is treatment using stem cells, in particular mesenchymal stem cells.
There is a method of treatment of inflammatory periodontal diseases with the use of compositions based on cell cultures containing the fibroblast cultures, growing environment, osteoplastic material (U.S. Pat. Of the Russian Federation No. 2210352). As of fibroblast culture it contains a strain of diploid lung cells of a human embryo LAC-4(81), as the medium growth medium NEEDLE-MEM with the addition of 10%serum fruits cattle, as osteoplastic material Hapka or Callahan. When using the known method possible immune reaction in the patient. And the most important clinical and histological results after such interventions are highly ambiguous: is formed defunct the national scar, celebrated the expansion of the boundary of the epithelium and the absence of normal periodontal attachment in the area of healing of periodontal tissues, in the substituted section of the bone is marked excess calcification with impaired skeletal tissue functions, often observed reaction to the foreign body until the pyrogenic effects. There is a risk of human transmission of infections in animals.
There is a method of treatment with mesenchymal stem cells (MSCS) (U.S. Pat. Of the Russian Federation No. 2265445). In this way, the suspension of MSC or chondroprotective cells or combinations thereof is administered intraligamentary with the lateral and medial sides of each affected caries of the tooth and/or subperiosteal in periodontal sites of the upper and lower jaws. Mesenchymal stem cells by this method is obtained from fetal donor material or from the recipient's own tissue (for example, from bone marrow, adipose tissue). As the cell material used alien chondroprotective cells, sometimes in combination with alien MSC. Made of MSC slowly and not fully acquire functional properties of osteocytes. The treatment period of 6 months, and orthopantogram significantly increase the optical density of the bone of the alveolar process compared to the "before treatment" 49±3%, p=0.05. In comparison with the known SPO is obom autologous MSCS have advantages, because eliminate the immune response, blood-brain reaction. In the combined treatment as cellular composition, and enter the volume of the cell suspension.
The closest method of treatment of periodontitis is the way to restore the bone of the alveolar ridge of the jaw bone and periodontal tissues with reduced capacity, including the removal of pathologically altered structures, the filling of the defect with a blood clot from the jaw bone and/or periodontal ligament. In the area of tissue defect transplanted autologous vascular cell fraction containing mesenchymal stem cells - fibroblast-like cells isolated from adipose tissue and purified from Mature adipocytes, without prior cultivation, and when the recession of periodontal tissues exposed root surfaces of the teeth previously covered palatal podperevalny connective tissue flap (U.S. Pat. RF 2320285). When using this method cannot eliminate the risk of neoplastic processes and reduce the time of treatment.
The objective of the present invention is to improve the efficiency of recovery expressed periodontal defect associated with periodontal disease, the reduction of treatment time.
The essence of the invention is a method for restoring the Oia bone and periodontal tissues, including the removal of pathologically altered structures and introduction to defective cavity mesenchymal stem cells suspended in the medium. As stem cells using mesenchymal stem cells from umbilical cord or placenta embryo obtained after a normal birth, with carry introduction cells by injection of 1-2 ml of blood plasma of the patient containing 5-10·106cells.
Example 1. Obtaining mesenchymal stem cells
Mesenchymal stem cells of the umbilical cord or placenta were obtained in accordance with the standard method as follows. Samples of the umbilical cord or placenta were collected after normal delivery at 39-40 weeks of gestation. Vein of the umbilical cord and placenta were Coulibaly and washed first with Hanks solution, and then 0.1% solution of collagenase type I, prepared in DMEM without serum, and incubated at 37°C for 30 minutes and Then the vessels were again washed with Hanks solution, the fabric was subjected to a short soft mechanical stress and collecting the separated cells. The obtained cells were besieged by centrifugation. Sediment resuspendable in DMEM containing 10% serum, 100 units/ml penicillin, 100 units/ml streptomycin, 2 mm glutamine, 1 mm sodium pyruvate, 10 ng/ml basic fibroblast growth factor (β-FGF), was transferred into the culture Cup and cultivar the Wali before the formation of the monolayer, changing environment 2 times a week. When reaching the monolayer cells were perseval in the ratio of 1:3. In the case of cell culture in clinical trials of the method after the last subculture under cultivation instead of fetal calf serum used the serum obtained from the patient's own blood.
Example 2. Laboratory tests on animals.
The method is tested in experiment 16 rats in the clinic for 7 patients. Of male rats weighing 300-350 g were subjected to light anesthesia. Periodontitis caused by the imposition of a pressure ligatures of the nylon thread around the neck of the first lower right molar. 16 rats 7 days after applying ligatures in the gum was introduced 500 thousand cells in sterile saline R-re. 8 control animals in the gums were injected with physiological R.-R. Even after 2 weeks, all animals were killed and subjected to the study of the periodontium and alveolar bone in the area 1 lower left molar. Control animals were observed pronounced inflammation of the periodontium and the signs of destruction of the alveolar bone, as well as increased activity of metalloproteinases. 14 experimental animals, these effects were completely absent, and the two were poorly marked.
Experiment on animals showed a significant acceleration of the process of primary wound healing when using mesenchyme is lnyh stem cells of the umbilical cord or placenta embryo received after normal delivery.
Example 3. In clinical trials.
The patient is complaining of tooth mobility. According to the examination 2-3 degree, bleeding gums. On the basis of clinical and rentgenologicheski examination diagnosed with periodontitis severe atrophy more than 1 of the alveolar ridge. The patient held: professional hygiene of the oral cavity, the teeth of the upper and lower jaw siniawan selectively thorough curettage of pathological pockets. After the above mentioned treatment on the basis of voluntary informed consent of the patient conducted substitution of cell therapy in accordance with the claimed method of treatment using injections of a patient's blood.
When using the inventive method in clinical trials were also obtained positive results. Introduction cells was performed by injection of 1-2 ml of blood plasma of the patient containing 5-10·106cells. All 7 patients up to 5 days decreased periodontal pockets, disappeared breath, stopped bleeding gums, mucous membrane bought a pale pink color, i.e. was visualized positive dynamics of the disease. When rentgenologicheski control after 6 months was determined by the growth of bone tissue, reducing the depth and width of the periodontal karmano is, what is the evidence of remineralization of bone tissue. Tooth mobility was gone.
The use of stem cells mesenchymal stem cells of the umbilical cord or placenta embryo received after normal delivery, helped to increase the effectiveness of treatment, to increase the reproducibility of results and to avoid unwanted effects.
Method of recovering bone and periodontal tissues, including the removal of pathologically altered structures and introduction to defective cavity mesenchymal stem cells suspended in the medium, characterized in that as stem cells using mesenchymal stem cells from umbilical cord or placenta embryo obtained after a normal birth, with carry introduction cells by injection of 1-2 ml of blood plasma of the patient containing 5-10·106cells.
SUBSTANCE: invention relates to medicine, namely to dentistry, and can be used for determination of periodontium state. For this purpose amount of gingival fluid in dentogingival groove or periodontal recess in area of all present teeth is determined, obtained values are compared with determined regularity for each tooth. After conclusion about severity and spread of pathologic process therapeutic measures are taken and conclusion about impact of taken measures on patient's periodontium is made.
EFFECT: method makes it possible to determine periodomtium state both in patients with complete dentitions and those with defect of dentition integrity, characterising state of tissues of dentition periodontium in general and of each tooth separately, being available and of low cost.
2 ex, 3 tbl
SUBSTANCE: group of inventions relates to field of preparations for oral cavity hygiene. Claimed is composition for teeth care, which contains dispersed abrasive substance from elementary silicon. In composition porous silicon or porous silicon with modified surface can be sued. Average size of particles of silicon abrasive preparation usually constitutes from 1 to 100 mcm. Also claimed are method of said composition obtaining, method of reduction of dental deposit formation with application of said composition and application of composition for production of medicinal composition for reduction of dental deposit formation. Silicon particles are capable of adhesion to teeth surface, which ensures a number of useful properties of composition for dental care, which can be made in form of tooth paste, tooth powder, prophylactic paste, lozenges, pills, drops, chewing gum of gel for oral cavity.
EFFECT: elaboration of efficient composition for tooth care.
32 cl, 5 ex, 13 dwg
SUBSTANCE: invention relates to dentistry, namely to means for teeth care, oral cavity care and prevention of dental diseases. Edible chewing gum with wound-healing effect contains agar, sweetener, extracts of medicinal plants, hydrophilic sorbent, emulsifier, food colouring and water, as extracts of medicinal plants it contains extract from leaves of nettle, yarrow, coltsfoot, as hydrophilic sorbent - apple pectin, as emulsifier - glycerylmonostearate, additionally it contains essential oil of mint, extract of karotenoids from fresh tomato raw material with specified component ratio.
EFFECT: invention provides edible chewing gum, possessing expressed wound-healing, antimicrobial and deodorating activity, as well as non-suppressing saprophyte flora of oral cavity.
SUBSTANCE: group of inventions refers to medicine, namely to dentistry, and aims at desentisation, reduced calculus formation, caries and bad breath. An oral cavity contacts with an oral composition containing an active ingredient consisting of a zinc citrate agent and a potassium citrate agent where the composition has pH value ranging within approximately 6 to approximately 10.
EFFECT: due to the presence of potassium citrate combined with zinc citrate, the inventions provides to reduce viscosity of the composition with preserving a required antibacterial and desensitising effect, to ensure desentisation, to reduce calculus formation, caries and bad breath.
21 cl, 1 ex
SUBSTANCE: invention relates to novel carbostyril compounds of general formula (1) or salts thereof with common pharmaceutically acceptable acids or pharmaceutically acceptable basic compounds, having activity on promotion of TFF2 production, a pharmaceutical composition based on said compounds, an agent based on disclosed compounds used in case of a disorder where up-regulation of TFF has a prophylactic and/or therapeutic effect, use of disclosed compounds to prepare said agent and a method of producing disclosed compounds. The invention also relates to novel specific carbostyril compounds or salts thereof with common pharmaceutically acceptable acids or pharmaceutically acceptable basic compounds. In structural formula (1), A is a direct bond, a lower alkylene group or lower alkylidene group, X is an oxygen or sulphur atom, the bond between positions 3 and 4 of the carbostyril backbone is a single bond or a double bond, R4 and R5 each denotes a hydrogen atom provided that, when the bond between positions 3 and 4 of the carbostyril backbone is a double bond, R4 and R5 can instead be bonded to each other in form of a -CH=CH-CH=CH- group, and R1, R2 and R3 assume values given in the claims.
EFFECT: high efficiency of compositions based on said compounds.
32 cl, 23 dwg, 184 tbl, 1535 ex
SUBSTANCE: method includes biomodification of surface of tooth root cement, application of osteotropic material and cover membrane. Recipient zones are processed by preliminarily prepared, immediately before operation, solution of 10000 U of human leukinferon in 5 ml of water for injections. The same solution is used to soak osteotropic material and cover resorbable membrane for 10-15 minutes. After that, bone defects are filled with prepared osteotropic material, membrane is fixed. When operation is completes, gauze bands, soaked with remaining solution, are applied on wound surface for 20-30 minutes.
EFFECT: method makes it possible to prevent early inflammation complications and disease recurrence, prolong remission terms, obtain high cosmetic and functional results.
FIELD: medicine, pharmaceutics.
SUBSTANCE: invention relates to chemical-pharmaceutical industry, in particular, to phitosubstance with antimicrobial and anti-inflammatory properties. Composition of phitosubstance with antimicrobial and anti-inflammatory properties contains eucalyptus leaves, calendula flowers, English oak bark, Echinacea purpurea herb, clove oil (eugenol), 40%-70% ethyl alcohol, with specified component ratio.
EFFECT: medication possesses expressed antimicrobial and anti-inflammatory action.
1 tbl, 1 ex
FIELD: medicine, pharmaceutics.
SUBSTANCE: invention relates to field of dentistry and represents therapeutic and preventive medication for dental enamel, which is characterised by the fact that it contains histidine 0.001-1.0%, lysine - 0.01-2.0%, arginine - 0.1-5.0%, each of said amino acids from total weight and distilled water - 20-90% of total weight.
EFFECT: invention makes it possible to prevent caries and normalises exchange processes in solid dental tissues.
21 cl, 5 dwg
SUBSTANCE: invention relates to field of dentistry, namely, to compositions for teeth and oral cavity care. Therapeutic-preventive composition contains sodium fluoride, sodium lauryl sulfate, lauryl glucosi, cocamidopropyl betaine, polyvinylpyrrolidone, panthenol, phytopain, propolis extract, saccharin, fragrance and water with definite component ratio.
EFFECT: composition is an effective cleaning and anti-caries preparation, which does not require application of tooth brush and procedure of further mouth rinsing; is characterised by mild action, does not damage and does not irritate teeth and oral cavity tissues; possesses high bactericidal and anti-inflammatory properties.
SUBSTANCE: claimed invention relates to field of medicine, namely to dentistry, and can be used in treatment of patients with chronic generalised periodontitis. Method includes removal of over- and under- gum dental plaques, application in area of upper and lower jaws of periodontal dressing with gel, containing active substance in amount 5% and base, including polyethylene glycol 400. Dressings are applied until inflammation in periodontium tissues is relieved, applying gel, which contains as 5% active substance dibunol and additionally as active substance chlorhexidine gluconate in form of 20% solution, and as base, it additionally contains glycerol, Vaseline oil, cremophor RH-40, aerosil and purified water.
EFFECT: application of invention makes it possible to create high medication concentration for short term with further effect of medicinal action prolongation, including etiotropic, antioxidant and immunocorrecting action.
2 cl, 3 ex
SUBSTANCE: disclosed is a method of obtaining and purifying human inhibin-A. Pieces of placenta are crushed and homogenised. A physiological solution is added in ratio of 1:2. Butyl alcohol is added to the obtained mass in ratio of 1:10 and then left for a day in a refrigerator at t +4°C. The mixture is then centrifuged with diethyl ether in ratio of 1:3 for 10-12 minutes at 5000 rpm in a cold centrifuge twice. Supernatant fluid containing inhibin is put into a flask which is then put into a refrigerator at t +4°C for a day. The supernatant fluid is centrifuged once more and over protein content therein is determined. The obtained solution is mixed with an equal volume of saturated ammonium sulphate solution and centrifuged in a cold centrifuge for 45-50 min at 10000 rpm. The obtained residue is dissolved in a tris-HCl buffer with pH 7.8, and then deposited on a lectin-sepharose column balanced with tris-HCl buffer. The entire volume of the dissolved residue obtained at the previous step is passed through. The column is washed with 1M sodium chloride solution buffered with tris-HCl buffer. The inhibin bound on the column is then eluted with 0.1M lactose solution in a borate buffer at pH 9.0. The eluate undergoes dialysis and concentration.
EFFECT: method enables to obtain inhibin-A with high output, high specific activity and high degree purification.
1 tbl, 3 ex
SUBSTANCE: invention relates to veterinary science. The method provides subcutaneous or intramuscular introduction of complex homoeopathic preparations Mastometrin, Traumel C, Echinacea compositum and Mucoza compositum 5 ml, 2.2 ml, 1.1 ml and 1.1 ml respectively 2-3 times a week. In addition, a biogene stimulator 5 ml in the form of a denaturated emulsified placenta is simultaneously introduced.
EFFECT: method allows reducing length of treatment.
SUBSTANCE: jejunal epithelium repair in old laboratory animal exposed to ionising radiation is ensured by intravenous transplantation of allogenic multipotent mesenchymal stromal cells recovered from placenta in amount 6×106 cell/kg 25-30 min after the exposure.
EFFECT: invention allows extending the range of products able to maintain repair tissue potential, namely antiapoptogenic action, higher cell turnover of crypt jujenal epithelium depressed by the ionising radiation exposure, with no therapeutic complications.
SUBSTANCE: invention relates to medicine, in particular to cosmetology. Anti-eczema medication contains propylene glycol extract of medicinal herbs of common tormentil, Achillea millefolium, Aloe arborescens, German chamomile, St John's wort, three-lobe beggarticks taken in definite ratio, polypropylene extract of pig placenta and, cyclomethicone DC 345, elastomer DC 9045, avocado oil, emulsifier DC 5329, acrylate emulsion of copolymer Salcare SC80, triethanolamine, fragrance, preservative Kathon CG and slightly mineralised spring water, with definite component content.
EFFECT: medication is efficient in case of eczema, possesses anti-inflammatory and bactericidal action, regenerates, enhances skin tone and elasticity.
1 tbl, 3 ex
FIELD: medicine, veterinary science.
SUBSTANCE: invention refers to veterinary science. The method involves three intramuscular introduction of ferroglucin 75 mg (1 ml) every 5 days, three intramuscular introduction of phosprenyl by different syringes in different points simultaneously with an iron preparation, 0.12 ml/kg for the first injection, 0.10 ml/kg for the second injection, 0.08 ml/kg for the third injection, and the intramuscular injections of gamavit 0.018 ml/kg once a day for eight days starting with the first injection of ferroglucin.
EFFECT: method allows to prevent vascular complications in newborn calves with anaemia, to optimise microcirculation and tissue trophism, to hasten the growth, to improve a herd, to produce greater amount of meat production, to produce an expected healthy posterity from these animals.
FIELD: medicine, veterinary science.
SUBSTANCE: invention refers to veterinary science. The method involves prescription of two intramuscular injection of ferroglucin 150 mg (2 ml) every 10 day, three intramuscular injections of phosprenyl in different syringes into different points, 0.12 ml/kg for the first time together with an iron agent, 0.10 ml/kg five days after the first injection of ferroglucin, 0.07 ml/kg for the third time together with the second injection of ferroglucin and intramuscular injections of gamavit 0.020 ml/kg once a day for eight days starting with the first injection of ferroglucin.
EFFECT: method allows to normalise spontaneous erythrocyte aggregation in suckling calves with anaemia, to provide higher additional weights, to reduce mortality and to produce an expected healthy posterity from these animals.
SUBSTANCE: invention relates to pharmacology. Medication for brain cell protection contains dominant protein extract from placenta tissue, obtained by chromatographic separation of protein fraction of 8-10 components with total molecular weight from 15 to 200 kDa, 70-80% of fraction consisting of protein with molecular weight about 70 kDa. In order to obtain said medication powdered placenta is extracted by means of alkalescent buffer in presence of inhibitors of proteolytic protein cleavage and centrifuged. Centrifugate is passed through chromatographic column with anion-exchange carriers balanced by means of the same buffer, column is washed from unbound material, proteins are eluted with neutral salt solution. Collected material is diluted with water bringing pH to subacid value and diluted substrate is successively passed through column with anion-exchange carrier and column with cation-exchange carrier, balanced with buffer solutions to pH about 6.0. Final product is obtained after sterilisation.
EFFECT: method application allows to obtain efficient medication of high purity, normalising nervous system functions.
3 cl, 1 tbl, 4 ex
FIELD: medicine, veterinary science.
SUBSTANCE: invention refers to veterinary science. The method involves three intramuscular introduction of ferroglucin by 75 mg (1 ml) every 6 days, three intramuscular introduction of phosprenyl by different syringes in different points simultaneously with an iron preparation, 0.10 ml/kg for the first injection, 0.08 ml/kg for the second injection, 0.07 ml/kg for the third injection, and the intramuscular injections of gamavit 0.017 ml/kg once a day for eight days starting with the first injection of ferroglucin.
EFFECT: method allows normalising the level of spontaneous thrombocyte aggregation, helps to avoid vascular complications in newborn piglets with anaemia, to optimise microcirculation and tissue tropism, to accelerate growth of animals, to improve a herd, to provide higher quality and amount of produced meat, to produce a healthy posterity.
FIELD: medicine, veterinary science.
SUBSTANCE: invention refers to veterinary science. The method involves the introduction of 1% novocaine, denatured emulsified placenta, nettle tincture and Endometromag T. 10 minutes after foetus birth, 1% novocaine is introduced in dosage 100 ml with puncture procaine block assistance, and for the 6th day after calving, novocaine is introduced once again in the same dose; denatured emulsified placenta is introduced suncutaneously in dosage 20 ml immediately after calving and 72 hours after calving; nettle tincture prepared by covering nettle with boiling water in the ratio 1:20 is introduced in dosage 5 l for the first days and for the next 3 days running, and Endometramag T is administered intrauterinally on the first days after calving in dosage 100 ml.
EFFECT: method is effective in the prevention of postnatal subinvolution of uterus in cows, allows accelerating lochia elimination from uterus to be prepared for a new pregnancy.
2 tbl, 1 ex
SUBSTANCE: invention relates to veterinary. Method of obtaining placental medication on the basis of cows' placenta is characterised by the following: in period of evisceration of incalvers at slaughterhouse, pregnant uterus is removed in the first half of pregnancy (4-5 months), non-separated caruncles and cotyledons are collected from baby's and maternal placenta by cutting cotyledons with curved sterile scissors from baby's placenta, which is made through longitudinal cut in serous-muscular-mucous layer of pregnant uterine horn, puncturing with a needle chorionically amniotic membrane by vacuuming amniotic fluid is collected from amniotic bubble; cut off cotyledond are crushed with caruncles by cutting, after that in mechanical homogeniser, amniotic fluid, previously collected from the same uterus, is added in amount 10:1 of homogenous mass, slowly mixed and settled, after that centrifuged at 10 thousand rev/min for 10 minutes, conservation is performed, supernatant part is poured into vials and sealed.
EFFECT: invention ensures increase of preparation efficiency.
SUBSTANCE: method of obtaining medications from fetus umbilical cord lies in the following: from calf fetus in the first part of intrauterine development umbilical cord as amputated in area of umbilical ring and on border of amnion-chorionic part of fetus placenta, blood is discharged from umbilical veins and arteries, amputated end of umbilical cord are fixed, umbilical blood is separated, components are separated, passed through nano-filters, amputated umbilical cord is disinfected and Wharton's jelly is separated, umbilical arteries, veins, umbilical envelope are separated and fixed at specified conditions, obtained materials are frozen in liquid nitrogen.
EFFECT: method makes it possible to increase purity of obtained preparation without application of chemical reagents.
1 tbl, 3 dwg