Synthetic nutrient medium for growing microorganisms
SUBSTANCE: synthetic nutrient medium for growing microorganisms contains citric acid, asparagine, potassium phosphate twice substituted, zinc sulfate, magnesium sulfate, iron sulfate, sodium chloride, sodium phosphate twice-substituted, glycine, succinic acid, distilled water and if necessary agar with specified component ratio.
EFFECT: invention makes it possible to increase nutritional properties of synthetic nutrient media.
2 cl, 1 tbl, 3 ex
The invention relates to Microbiology and biotechnology, in particular to the design of synthetic environments for cultivation of mycobacteria of different species, Pseudomonas molleo, Pseudomonas aeruginosa Pseudomonas aeruginosa, Staphylococcus, Salmonella, E. coli, Proteus, etc. as well as probiotic microorganisms.
On the origin of the nutrient medium is divided into natural (blood, milk, potato, egg, artificial using the universal source of nitrogen and carbon - peptone obtained by incomplete cleavage of proteins by enzymes (pepsin, trypsin) is different hydrolysates: fish, casein, meat, yeast and synthetic nutrient medium prepared from balanced chemical components (Korotyaev A.I., Babichev S.A. Medical Microbiology, immunology and Virology. SPb., 1998. - P.78-79).
At the same time when producing staphylococcal, komisarkaplan of intoxination, mallein for the purpose of humane and veterinary medicine cultivation of the microorganisms is performed in environments that use mesopatamia-potato broth or hydrolizates meat, fish, casein, characterized by instability of the composition, uneven growth and fibre products.
Known synthetic nutrient medium Kursk Biofactory (p the test environment Engleskog # 1 and # 2) are used for the cultivation of mycobacteria of different species in the manufacture of diagnostic allergens (A.A. Iglewski Experimental rationale and practical aspects of diagnosis and prevention of infectious and allergic diseases of animals. Abstract. dis. ... Prof. wet. Sciences, Lovetension, 1990. - P.8-9).
However, these synthetic environment versatility not possess. And the content of zinc sulfate 0.1 g/l does not always ensure surface cultivation of Mycobacterium tuberculosis. Known environment have also composed of scarce ammonium citrate.
Almost cultivation of Mycobacterium tuberculosis is carried out with the use of synthetic environments to obtain TB allergen - tuberculin PPD instead NKGB. Synthetic environment do not contain dietary fibre meat, casein, peptone, and were first used to obtain PPD-tuberculin (F.Seibert in 1934, Maslennikova in 1939, Ask in 1963)
Known synthetic nutrient medium for cultivation of Mycobacterium tuberculosis (patent No. 2139345, 10.10.1999, author ISI) includes, wt.%: citric acid 6,0±2,0; asparagine 7,5±1,5; ammonium citrate 3,0±1,0; sulfuric acid magnesium 0,75±0,15; iron sulfate 0,075±0,015; zinc sulfate 0,15±0,075; dvuhkamernyi phosphoric acid potassium 7,5±1,5; glycerol - rest.
However, this synthetic environment suitable for growing only Mycobacterium tuberculosis, that is, has no versatility, PR is the suitability for growing number of other pathogenic and probiotic microorganisms in the manufacture of biological products.
The aim of the invention is to increase the nutritional properties of the synthetic environment suitable for stable growth and maximum accumulation of a number of pathogenic and probiotic microorganisms.
This goal is achieved by increasing the content of citric acid prior to 9-11 g/l, zinc sulfate 0.5 g/l, more making succinic acid 3.0 g/l, sodium phosphate 2-substituted 1,5 g/l, glycine 1.0 g/l, NaCl 0.5 g/l, a decrease in the content of expensive asparagine to 2-4 g/l, and the complete withdrawal of scarce ammonium citrate.
Developed synthetic environment contains chemical ingredients in the following proportions, in grams per 1 liter of distilled water, citric acid 9,0-11,0; asparagine - 2,0-4,0; potassium phosphate dvuhkamernyi - 6,0; zinc sulfate is 0.5; magnesium sulfate - 0,7; sulphate of iron - 0,1; sodium chloride and 0.5; sodium phosphate dvuhkamernyi - 1,5; glycine - 1,0; succinic acid - 3.0 and glycerin 40-50 ml.
The reaction medium is set 5-10% ammonia solution to pH 7.0, and 7.1 before autoclaving, and for the cultivation of staphylococci to pH 7.5 and 7.6 with the increase of sodium chloride up to 5-6 g/l
Introduction citric and succinic acids (di-, and tricarboxylic acid cycle Krebs) increases metabolism in microorganisms, improving the solubility of x the chemical ingredients and the formation of ammonium citrate by neutralizing citric acid 5-10% solution of ammonia. The formation of citrate of ammonium as nitrogen compounds needed to build protein molecules of microorganisms, improves metabolism and growth of microbial biomass. Introduction in the nutrient medium composition of glycine in combination with asparagine (amide of aspartic acid) improves the biosynthesis of protein structures of microorganisms, and sodium chloride and sodium phosphate dvuhkamernyi increase the buffering capacity of the environment help to keep the pH during prolonged cultivation of Mycobacterium tuberculosis and Staphylococcus.
The proposed composition of the medium differs from the prototype and analogues qualitative and quantitative ratio of ingredients and provides the accumulation wrung dead Bakassi of Mycobacterium tuberculosis to 130±10.0 g/l after 60 days of cultivation, microbial cells of Salmonella and E. coli after 2-3 days of incubation up to 70±10,0 billion/ml, Staphylococcus after 10-12 days of growing up to 13±1.0 billion/ml, probiotics after 2-3 days of cultivation 70-90 billion/ml Thus, the claimed universal synthetic environment meets the conditions of patentability of "novelty" and "inventive step".
Synthetic nutrient medium, providing consistently high accumulation of microorganisms with subsequent receipt of them tuberculin PPD, TB toxoid, mallein, to bacterioses, komisarkaplan, staphylococcal toxoid vaccines, etc. meets the condition of patentability "industrial applicability".
Synthetic medium for the cultivation of microorganisms was obtained by serial dilution in distilled water ingredients or dissolving them with advanced components are mixed in dry form in the ratio, g/l, citric acid 10.0 g; asparagine 3,0; potassium phosphate dvuhkamernyi 6,0; zinc sulfate to 0.5; magnesium sulfate 0,7; sulphate of iron of 0.1; sodium chloride of 0.5; sodium phosphate dvuhkamernyi 1,5; glycine 1,0; succinic acid 3,0; glycerol 50 ml and subsequent neutralization of the environment 5-10% solution of ammonia and the reduction of the volume of liquid medium by adding distilled water to 1 liter.
For the cultivation of staphylococci in the composition of the synthetic environment, the content of sodium chloride brought to 5 g/l while maintaining the above ratio of all other components, and pH to 7.5 in connection with the physiology of this species of microorganisms.
In the preparation of dense fundamentals of synthetic environments diluted with distilled water in the ratio 1:1 and add 2.5% agar, followed by sterilization at 100°C for 30-60 minutes and packaged in tubes, Petri dishes for sealing and further use for growing microorganism is.
The invention is illustrated by the following examples.
Example 1. Determination of the nutritive value of the synthetic nutrient medium during the cultivation of Mycobacterium tuberculosis.
The studies have used laboratory and industrial strain No. 8 of Mycobacterium tuberculosis and freshly isolated microorganisms.
Seeding of microorganisms was performed using a platinum loop (spiral) on the surface of the liquid synthetic environment. During incubation for 20-30 days there was a full coating film of Mycobacterium tuberculosis entire surface of the medium diameter up to 0.5-0.7 cm, and after 60 days the thickening of the film reached 1.0 cm or more. After autoclaving grown Mycobacterium tuberculosis with the subsequent release of Bakassi through several layers of gauze and drying were weighed.
On average, a constant accumulation of terraria (wrung through several layers of gauze) Bakassi Mycobacterium tuberculosis was 120-130 g/l, and the dry - 12-13 g/l
Example 2. Cultivation of Mycobacterium tuberculosis on solid agar medium.
In the synthetic environment of the claimed composition, as well as when breeding it with distilled water 1:1 was added 2.5% agar, sterilized at 100°C for 30-60 minutes, were Packed up in tubes and grew Mycobacterium tuberculosis. The growth of M. tuberculosis in PR the tags with agar occurs 7-10 days after seeding of microorganisms, as is the case with the stated concentration of the synthetic nutrient medium, and when it is diluted with distilled water 1:1. Thus, in the preparation of dense medium with the addition of agar prepared with liquid synthetic medium is diluted with distilled water in a 1:1 ratio.
Example 3. Test the effectiveness of the growth of staphylococci, Salmonella, and Escherichia coli (E. coli) on the universal synthetic environment and mineral-based in the stated concentration and dilution with distilled water 1:1 with 2.5% agar.
The concentration of staphylococci after 12±2,0 day cultivation in the two-liter beautylab with medium volume, equal to 1.0 liter, is 12±1.0 billion/ml of microbial cells and Salmonella and Escherichia coli after 2-3 days incubation - 70±10,0 billion/ml at continuous sprouting colonies of microorganisms agar surface tubes on a mineral basis. Cultivation of microorganisms on solid agar medium liquid mineral Foundation pre-diluted by half with distilled water. Lower concentrations in combination with 2.5% agar does not affect the growth of the above-mentioned microorganisms.
Examples confirmed the suitability of the nutrient medium in the proposed options for the cultivation not only of M. tuberculosis and other pathogenic microor the mechanisms are: Salmonella, Escherichia coli, Pseudomonas aeruginosa, Proteus, and probiotic microorganisms, i.e. its versatility. For the cultivation of staphylococci in the composition of the medium increase the content of sodium chloride up to 5-6 g/l and pH to 7.5 and 7.6 in accordance with the physiological requirements of these microorganisms.
Results accumulation of bacterial mass cultivation of microorganisms on the claimed synthetic nutrient medium is given in the table.
|The accumulation of bacterial mass cultivation of microorganisms on synthetic nutrient medium.|
|№ p/p||Name of microorganisms||Growing period||Accumulation (concentration) of microorganisms|
|1.||Mycobacterium tuberculosis (bovine species)||30 days||60 g of autoclaved and pressed Bakassi|
|60 days||120 g of autoclaved and pressed Bakassi|
|2.||Staphylococci (C is latesty)||7 days||7-8 billion/ml|
|12 days||12±1.0 billion/ml|
|3.||Salmonella||2-3 days||70±10 billion/ml|
|4.||E. coli||2-3 days||70±10 billion/ml|
|5.||Pseudomonas aeruginosa||2-3 days||Thick lisaadamsy|
|6.||Bacillus subtilis - Bacillus||2-3 days||Thick lisaadamsy|
|7.||Lactobacillus||2-3 days||Thick lisaadamsy weight|
1. Synthetic nutrient medium for exp is farming microorganisms,
including citric acid, asparagine, potassium phosphate dvuhkamernyi, zinc sulfate, magnesium sulfate, iron sulfate, glycerin, characterized in that it further comprises succinic acid, glycine, sodium phosphate dvuhkamernyi, sodium chloride and distilled water in the following ratio of components, g/l:
|phosphate potassium dvuhkamernyi||6,0|
|phosphate sodium dvuhkamernyi||1,5|
|distilleria the water output||rest|
at pH 7.0, and 7.1.
2. Synthetic nutrient medium according to claim 1, characterized in that the dilution with distilled water 1:1 further comprises 2.5% agar.
SUBSTANCE: method involves preliminary selection of concentration of a disinfectant solution at which bacteria exhibit partial sensitivity to said disinfectant. The bacteria are exposed to the disinfectant solution at the given concentration. Grown colonies of bacteria are collected after exposure to said disinfectant and then sown on a solid culture medium, followed by culturing at 37°C. Bacteria colonies isolated from those grown at the previous step are selected in amount of 1-299 colony-forming units (CFU/ml). The collected isolated colonies are resown on a culture medium and grown at conditions needed for growth of bacteria of that type, followed by dividing the grown colonies into two parts. One part of the grown bacteria colonies is exposed to the disinfectant at the selected concentration, and the other part is exposed to the disinfectant at bactericidal concentration. If upon exposure to the disinfectant at bactericidal concentration, there is no growth of bacteria or their growth ranges from 1 to 299 (CFU/ml), then the cycle of selecting bacteria grown at the previous step, their resowing on the culture medium, growing colonies of said bacteria at conditions needed for growth of said type of bacteria and exposing the grown colonies to the disinfectant at the selected and bactericidal concentrations is repeated once more until after exposing the column to the disinfectant solution at bactericidal concentration, growth of bacteria on the culture medium is equal to 300 (CFU/ml), which indicates bacterial resistance to the disinfectant.
EFFECT: invention enables to simulate bacterial resistance to a disinfectant, evaluate build-up of resistance and bactericidal potential of hospital strains to disinfectants during their practical use.
1 tbl, 1 ex
SUBSTANCE: method provides test inoculation of a nutrient medium containing pancreatic fish flour hydolyzate, fermentative meat peptone, NaCl, Tween-80, CaCl2, sodium thiosulphate (Na2S2O3 × 5H2O), ferrous ammonium sulphate ((NH4)2SO4 × FeSO4 × 6H2O), sorbite, bromthymol blue, irgasan (DP-300), rifampicin, NaOH, agar and distilled water in the preset proportions. Pancreatic fish flour hydolyzate, peptone, NaCl, agar are dissolved with heating, sterilised at 121°C for 20 min and thereafter added in a hot medium of the other components specified above. The inoculations are incubated on the nutrient medium in aerobic conditions at temperature 37°C and/or 28°C for 24-48 h and assessed by the presence of black-centre green or dark grey colonies surrounded by a cloudy precipitate zone in the nutrient medium.
EFFECT: invention allows simplifying and providing higher specificity of Shewanella bacteria recovery and identification.
SUBSTANCE: invention refers to biotechnology, and concerns a lactic bacteria Lactobacillus reuteri DSM 17938 strain stimulating IL-10 production and hence CD4+CD25+TR-cell proliferation used for making a probiotic product. The probiotic product contains Lactobacillus reuteri DSM 17938 strain and additionally medium-chain triglyceride oil.
EFFECT: use of the probiotic product promoted a favorable effect on intestinal colic in a newborn baby.
3 cl, 3 dwg, 1 tbl, 2 ex
SUBSTANCE: inoculate contains in a mixture or in a combination, main L-cystein of formula HSCH2CH(NH2)CO2H and at least one B.animalis lactis strain. Said cysteine and at least one B.animalis lactis strain are contained or have the form of at least one frozen granule and/or at least one lyophilizate. The pH value of the solution produced by thawing of at least one granule and/or dissolution, of at least one lyophilizate in the relation making 1 to 2 g of the lyophilizate per 8-10 ml of H2O, makes at least 4. L-cysteine contains in the inoculate in an amount within 1 g per 1·1014 CFU to 1 g per 3.5·1010 CFU, of at least one B.animalis lactis strain or all used B.animalis lactis strains whereas the same are numerous. Using the offered inoculate provides stimulation of B.animalis lactis growth and/or metabolism on a lactic substratum to produce a fermented diary product.
EFFECT: high probiotic value of the product.
21 cl, 9 dwg, 3 tbl, 3 ex
SUBSTANCE: nutrient medium contains yeast water, beef hydrolyzate, sodium chloride, glucose, glycerin, sodium citrate, sodium metabisulphite and distilled water.
EFFECT: invention allows providing optimum conditions for brucellous microbe growth, replication in a transport nutrient medium at any distances.
SUBSTANCE: strain of bacteria Bacillus thuringiensis BIOS-1 VKPM B-10709, possessing insectoacaricidal activity against representatives of leaf-eating and sucking pests, such as representatives of orders Lepidoptera, Coleoptera, Homoptera, Thysanoptera and Acariformes, doing harm to crops, is deposited in All-Russian collection of industrial microorganisms (VKPM), Federal State Unitary Enterprise GosNIIgenetika under number B-10709.
EFFECT: invention makes it possible to increase mortality of representatives of leaf-eating and sucking pests, doing harm to crops.
6 tbl, 2 ex
SUBSTANCE: strain of Bifidobacterium longum is separated from bowels content of healthy baby and is deposited in Government collection of microorganisms of normal microflora (GKNM) FSIS "MNIIEM named after G.N. Gabrichevskiy Rospotrebnadzor " under registration number 230. Strain multiplies in various nutrition mediua with accumulation of production biomass with high concentration of bifidobacteria.
EFFECT: Bifidobacterium longum strain has acid-forming activity, antagonistic activity with respect to pathogenic and opportunistic microorganisms, ferments milk, which makes it possible to apply it for obtaining bifidocontaining production.
2 tbl, 5 ex
SUBSTANCE: method of indicating hospital strains of Pseudomonas aeruginosa, Staphylococcus aureus, Enterobacter cloacae involves identification of an isolated pure culture of bacteria, inoculating the identified bacteria on blood-meat infusion agar while washing accumulated bacteria - S.aureus - after 12 hours, and Exloacae and P.aeruginosa - after 8 hours with sodium chloride solution. Electrical resistance of the suspension of bacteria in the sodium chloride solution is determined at bacteria concentration of 500000 in 1 ml in a dc electric field with 2.8 V across the electrodes. If electrical resistance for P.aeruginosa bacteria ranges from 579 to 674 kom, S.aureus 545-642 kom, Exloacae 452-584 kom and epidemiological data on recording five or more disease cases from one infection source, the isolated infectious agent is considered a hospital strain.
EFFECT: invention cuts time for conducting laboratory investigations and detecting circulation of hospital strains in hospitals, use of the invention does not require intraspecific identification of bacteria.
2 tbl, 3 ex
SUBSTANCE: nutrient medium contains enzymeatic hydrolyzate of beef meat, sodium chloride, sodium phosphate bisubstituted, sodium sulfite, Karpuzidi stimulant and purified water in specified component ratio.
EFFECT: invention makes it possible to obtain quality nutrient medium for culturing microbial cells of plague microbe of vaccine strain EB in sufficient quantity during 48 h.
1 tbl, 3 ex
SUBSTANCE: method involves treating a water-based medium containing sulphate-reducing bacteria SRB in industrial aqueous systems of chemical production and oil refining. Inhibition of production of biogenic sulphide with SRB takes place as a result of synergetic action of a biocide component in a first concentration and a metabolic inhibitor in a second concentration. The biocide immediately destroys the first portion of SRB. The biocide component is selected from a group comprising aldehydes, amine-type compounds, halogenated compounds, sulphur compounds, salts of quaternary phosphonium and/or combinations thereof. The metabolic inhibitor inhibits growth of a second portion of SRB without its direct destruction. The metabolic inhibitor component is selected from a group comprising nitrite, molybdate, tungstate, selenate, anthraquinone and/or combinations thereof. Contact between the SRB and the biocide and metabolic inhibitor can take place continuously, intermittently or simultaneously.
EFFECT: method ensures efficient inhibition of production of biogenic sulphide with SRB during combined use of components in considerably lower concentrations than if the biocide or metabolic inhibitor was used separately.
25 cl, 2 dwg, 1 ex
FIELD: biotechnology, microbiology, medicine.
SUBSTANCE: invention relates to the strain Lactobacillus paracasei CNCM I-2116 used for diarrhea prophylaxis causing by pathogenic microorganisms. Supernatant of this strain culture elicits ability to prevent colonization of intestine with pathogenic microorganisms causing diarrhea also and this strain is designated for preparing agent used for prophylaxis and/or treatment of disorders associated with diarrhea. Agent for oral administration represents therapeutically effective dose of the strain L. paracasei CNCM I-2116 or supernatant of its culture and acceptable foodstuff. Invention provides the enhanced viability of the strain in its applying and effectiveness in prophylaxis of adhesion to intestine cells and invasion to intestine cells of pathogenic microorganisms causing diarrhea.
EFFECT: valuable medicinal properties of strain.
5 cl, 8 dwg, 10 ex
FIELD: biotechnology, microbiology, agriculture.
SUBSTANCE: the strain Lactobacillus buchneri 600 is isolated from barley grains at milkwax stage of ripeness. The strain Lactobacillus buchneri 600 is registered in 05. 08. 2002 year in the All-Russian State collection of microorganism strains used in veterinary science and animal husbandry at number Lactobacillus buchneri VGNKI 02.08.04-DEP and deposited in the collection 000 "Biotrof". Invention provides the more effective multiplication of the strain in maize ensilaging green mass and preserving flattened grains with enhanced formation of lactic acid, inhibition of putrid microflora that allows preparing fodder from vegetable raw with enhanced quality. Invention can be used in fodder production for ensilage maize green mass and preserving flattened grains.
EFFECT: valuable properties of strain.
3 tbl, 2 ex
FIELD: biotechnology, agriculture, microbiology.
SUBSTANCE: invention relates to a new isolated strain of Lactobacillus acidophilus 1660/08 as a producer of the protein fodder. The strain Lactobacillus acidophilus 1660/08 is obtained by the selection method and selected by its ability to form significant amount of crude protein and to accumulate the biomass. The strain is deposited in the VGNKI collection at number VGNKI-03.04.10.-DEP. Invention provides eliminating the environment pollution in producing the protein fodder, to enhance the specific protein yield, to reduce energy consumptions in preparing protein fodder, to simplify and to accelerate the process in its preparing, to simplify equipment fitting out and to utilize waste in manufactures using natural raw.
EFFECT: valuable properties of strain.
2 tbl, 10 ex
FIELD: biotechnology, microbiology, agriculture.
SUBSTANCE: the strain Lactobacillus plantarum 578/25 is obtained by method of step-by-step selection and selected by its ability to produce significant amount of crude protein and to accumulate the biomass. The strain is deposited in the VGNKI collection at number VGNKI-03.04.09.-DEP. Invention provides eliminating the pollution of environment in producing the protein fodder, to elevate the protein specific yield, to reduce energy consumptions in preparing protein fodder, to simplify and to accelerate the process of its preparing, to simplify apparatus equipment, to utilize waste in manufacturing using the natural raw.
EFFECT: valuable properties of strain.
2 tbl, 10 ex
FIELD: biotechnology, microbiology, dairy industry.
SUBSTANCE: the strain of microorganism Lactobacillus lactis VKPM B-8354 is prepared without using mutagens and genetic methods and shows resistance against broad spectrum of lactophages. The strain ferments effectively milk from different trading sorts, with broad range of fatness and different methods of thermal treatment. Individual specific properties of the strain allows its applying as a monostrain ferment. Curd obtained with applying the strain L. lactis VKPM B-8354 shows good organoleptic qualities, nonacid taste and homogenous consistence. The strain is suitable especially for plants with small volume of manufacture but with varied assortment.
EFFECT: valuable properties of strain.
3 tbl, 2 dwg, 5 ex
FIELD: biotechnology, biochemistry, microbiology.
SUBSTANCE: the strain Bacillus circulans B-65 a producer of cyclodextrin glucanotransferase is isolated and selected form the soil sample by culturing in nutrient medium with amylolytic activity 12.17 U/ml and cyclodextrinogenic activity up to 0.530 U/ml. Cyclodextrin glucanotransferase isolated from B. circulans B-65 shows the high degree for conversion of starch to cyclodextrins and this enzyme is specific for formation of β-cyclodextrin. Invention can be used in food industry for preparing cyclodextrins and cyclodextrin glucanotransferase used in different branches of industry.
EFFECT: improved isolating method, valuable properties of strain and enzymes.
9 cl, 8 tbl, 2 ex
FIELD: biotechnology, microbiology, veterinary science.
SUBSTANCE: for preparing a preparation cells of microorganism Escherichia coli VKM CR-322D is cultured in nutrient medium containing Hottinger's broth, glucose, yeast extract, manganese sulfate, potassium hydrophosphate, sodium chloride and tap water in the content of amine nitrogen 125-155 mg%. Glucose is added by batch portions in the process of culturing cells that is carried out at temperature 30-31oC at stirring and aeration for 10-12 h. Prepared cultural fluid containing 3 x 109 bacterial cells/ml is mixed with protective sucrose-gelatin medium and subjected for lyophilic drying. Dried mass is stored under nitrogen that enhances safety of viable cells in the preparation. Applying the preparation for prophylaxis and treatment of agricultural and domestic animals and poultries with gastroenteric diseases provides its effectiveness.
EFFECT: improved preparing method, valuable veterinary properties of preparation.
17 cl, 8 ex
FIELD: biotechnology, microbiology.
SUBSTANCE: method for preparing liquid lactobacterin involves regeneration, culturing passages of lyophilized culture and culturing ferment of lactobacilli in liquid lyophilized nutrient medium containing dry defatted milk enzymatic hydrolyzate with the content of amine nitrogen 1 485 mg%, 30.0 ± 3.0 g/l; yeast concentrated autolyzate, 110.0 ± 10 g/l; food agar, 0.8 g/l, and distilled water, up to 1 l. Culturing ferment is carried out up to accumulation of biomass of lactobacilli 109-1010 CFU/ml. Then 10-30% of supernatant liquid is removed from the ready product and replaced it with equal volume of fresh nutrient medium. Invention provides simplifying technology in preparing liquid lactobacterin and to elevate the storage period of viable lactobacilli. Invention can be used in producing probiotic preparations.
EFFECT: improved preparing method.
1 tbl, 3 ex
FIELD: biotechnology, food and medicinal industry, microbiology.
SUBSTANCE: the strain Bifidobacterium longum 379-IN is obtained by selection without using methods of genetic modification of the strain Bifidobacterium longum B379M and distinct by ability to utilize insulin. The strain is deposited in GKNM GU "MNIIEM named for G. N. Gabrichevskiy Russia Ministry of Public Health" at № 172. The strain shows high technological effectiveness, accumulates biomass with substrates of vegetable origin and artificial nutrient media for short periods with concentration of bifidobacteria, it elicits acid-forming and antagonistic properties with respect to pathogenic and putrid microflora. This allows its using in manufacturing bacterial preparations, biologically active supplements for food, fermented-dairy and nonfermented-dairy foodstuffs, ferments, hygienic and cosmetic agents providing probiotic effect and normalization of microbiocenosis in human body, among them in gastroenteric and urogenital tracts, cutaneous and mucosa integuments. Invention can be used in manufacturing bacterial preparations, biologically active supplements for food, fermented-dairy and nonfermented-dairy foodstuffs, hygienic and cosmetic agents.
EFFECT: valuable properties of strain, expanded assortment of similar agents.
FIELD: biotechnology, microbiology.
SUBSTANCE: invention relates, in particular, to preparing nutrient media used for culturing the plague microorganism vaccine strain and can be used in medicinal microbiology. The nutrient medium for culturing the plague microorganism vaccine strain comprises additionally as a stimulating additive sodium sulfite and as a nutrient base - soybean fruits enzymatic hydrolyzate in the following ratio of components, g/l: microbiological agar, 11.0-13.0; soybean fruits enzymatic hydrolyzate, 250.0-350.0; sodium chloride, 4.5-5.5; sodium hydrogen phosphate, 3.5-4.5; sodium sulfite, 0.0003-0.0005; distilled water, the balance. Invention provides enhancing the growth property of nutrient medium.
EFFECT: valuable properties of medium.