Method of rehabilitation after hypoxia causing brain dysfunction in laboratory animal models
SUBSTANCE: drug-free correction of structure functional brain dysfunction caused by severe hypoxia/ischemia is ensured by placing a model in 24 hours after severe hypoxia in a pressure chamber and hypoxic post-conditioning at atmospheric pressure 360 mm Hg for 2 hours every 24 hours for three days.
EFFECT: method extends the range of products for studying rehabilitation capabilities after hypoxia causing brain dysfunction.
The invention relates to experimental medicine and can be used for non-pharmacological correction of structural and functional disorders of the brain, caused by severe hypoxia/ischemia.
Over the next analogues accepted: neuroprotective way hypoxic preconditioning moderate hypobaric hypoxia , the method of postconditioning on the heart  and the brain , as well as the modes used to hypobaric hypoxic stimulation . Method  was developed by us earlier with the aim of increasing the tolerance of the brain to damaging factors and prevention of post-hypoxic and post-stress disorders. Method  based on the exposure of the organism to episodes of moderate hypobaric hypoxia chamber (360 mm Hg corresponds to "rise" to a height of 5 km, 2 hours, three times with an interval of 24 hours. Such exposure causes multilevel activation urgent genome-dependent adaptation mechanisms, which increase the adaptive capabilities of the brain and the whole body to adverse factors (hypoxia/ischemia, stress). Method  effective, easy to use (easy to control and dosed) and is based on natural non-invasive treatment (hypobaric hypoxia occurs in natural conditions during the ascent to the height), but it is used only for the prevention of Vozniknovenie what I pathologies, and not to correct them. The correction of postischemic cardiac diseases, in particular reducing the size of the hearth damage after a heart attack, aimed way ischemic postconditioning infarction , namely, that early after myocardial infarction is temporary occlusion of coronary vessels and creates a transient myocardial ischemia. This method is described in 2003 by the American physiologists, who showed that if after 60 minutes of coronarography dogs in the reperfusion period to conduct three sessions of 30-second coronaryocclusion, interspersed with 30-second intervals resume coronary blood flow, the myocardium becomes resistant to reperfusion injury. The area of damage was decreased by 44% compared with controls . Subsequently, the cardioprotective effect of ischemic postconditioning has been confirmed in studies on other animals and clinical observations on patients with acute myocardial infarction. A similar technique was proposed and brain . According to  early after ischemic stroke by bilateral "ligation" (occlusion) of the carotid arteries creates a transient ischemia of the brain (four 20-minute episode of occlusion and 30-s reperfusion), which reduces the size of the evoked potential in the area of stroke by 80%. The drawback is this method limit its application in practice, should be attributed to poor controllability, insecurity and invasiveness of such effects. Currently, the effects of hypobaric hypoxia is widely used in practice to hypobaric hypoxic stimulation of various diseases, however, this mode of adaptation or training - regular daily UPS to a height of up to 3.5 km (20-30 sessions) [4, 5, and others].
Objective improving the adaptive capacity of the organism undergoing the influence of adverse factors (severe hypoxia).
The essence of the proposed method of recovery after hypoxia, including three hypobaric hypoxia, is that the individual has suffered severe ischemia/hypoxia, then subjected to moderate hypobaric hypoxia (360 mm Hg) duration : 2 hours in each of the next three days with an interval of 24 h, which greatly reduces the amount of neuronal damage and restores their function.
The claimed method is performed as follows. Hypoxic postconditioning (360 mm Hg is equivalent to the height of 5000 m, 2 hours with an interval of 24 hours) in the chamber flow type is carried out within 3 days after severe hypoxic stroke.
Figure 1 and 2 presents the results of the preclinical testing of the proposed method obtained in fashion is lnyh experiments on laboratory animals (rats).
The experiment demonstrated that hypoxic postconditioning in accordance with the stated Protocol significantly reduces the degree of neuronal brain damage caused by hypoxia stroke (severe hypobaric hypoxia - 180 mm Hg, equivalent to an altitude of 11,000 m).
Figure 1 survival Analysis of neurons sensitive to hypoxia of the brain area - CA1 field of the hippocampus after severe hypoxia (black bar) and severe hypoxia with postconditioning (grey column). White column control. * - differences from control are statistically significant, p≤0.05. Y - axis the number of surviving neurons.
Severe hypoxia induces extensive neuronal loss in the hippocampus and neocortex of rats. The loss of neurons in the most vulnerable areas - CA1 field of the hippocampus to 7 days after the damaging effects reached 32% of the total number of cells. Postconditioning contributes vyjivaniyu% of neurons in the neocortex and CA4 field of the hippocampus, and in the CA1 killed only 9% of neurons, and 32%as not-postconditioning individuals.
Along with this postconditioning has a pronounced anxiolytic effect on behavior, reducing the anxiety levels of individuals after severe hypoxia.
Figure 2 Postconditioning has a pronounced anxiolytic effect, reducing pathological anxiety in animals after heavy is OI hypoxia, manifested in the avoidance of open and lit places.
Legend: white column control; black bar - severe hypoxia; grey column - severe hypoxia with postconditioning. * - differences from control are statistically significant, p<0.05
And the behavior of animals in open-field test. Y - axis the number of crossed-lit squares in the center of the "open field", 5-th day after hypoxic insult;
B - behavior of animals in the test, the elevated plus maze". Y - axis time (s) stay in the open, lit sleeves elevated cross maze, the 6th day after hypoxic stroke.
Individuals who suffer severe hypoxia, characterized by a sharp decline paces in the middle of the "open fields" and the time spent in the open arms of the maze, indicating that the increase in their level of anxiety. Postconditioning prevents the development of this pathological condition and normalizes the behavior of individuals, providing a pronounced anxiolytic effect.
Thus, the application of the proposed method provides structural and functional rehabilitation of the brain after severe hypoxia.
The characteristics of the prototype , which coincides with the essential features saleemul of the invention: parameters of moderate hypobaric hypoxia - 360 mm rtsenable 5000 m, 2 hours with an interval of 24 hours. Differences from the similar  - exposure moderate hypobaric hypoxia after damaging impact, i.e. in the mode of postconditioning.
Similarly,  and  in the proposed method uses the mode of postconditioning, but is used as postconditioning factor not ischemic, and combined hypoxic/hypobaric exposure.
Signs analogues  and , coinciding with the essential features of the claimed invention: the use of hypobaric hypoxia in a pressure chamber. Differences from analogues  and  - parameters hypoxia (high altitude "UPS") and the number of sessions (3 and not 20-30).
Thus, the claimed technical solution meets the criterion of "novelty", as it has significant distinguishing features of the prototype and model, thanks to which we can make a conclusion about conformity of the proposed method inventive step.
Sources of information
1. Samoilov MO, Rybnikova E.A., Tjulkov H., Wateva L.A., Amelin VA, Hogy LI, Pelto-Hucka M. the Effect of hypobaric hypoxia on the behavioural responses and the expression of early genes in the rat brain: corrective effect preconditioning impact// Reports of Academy of Sciences. 2001, 381, 1, 1-3.
2. Zhao ZQ, Corvera JS, Halkos ME, Kerendi F, Wang NP, Guyton RA, and Vinten-Johansen J (2003) Inhibitin of myocardial injury by ischemic postconditioning during reperfusion: comparison with ischemic preconditioning. Am J Physiol 285: H579-H588.
3. Zhao H., Sapolsky R., Steinberg G. (2006) Interrupting reperfusion as a stroke therapy: ischemic postconditioning reduces infarct dementia size after focal ischemia in rats. 166: J. Cereb. Blood Flow. Metab. 26 (9): 1114-1121.
4. The method of adaptation to chronic intermittent hypobaric hypoxia in therapy and prevention. Methodical recommendations. - M.: the Ministry of health of the RSFSR, 1989.
5. Chieftains A.A., Buoys, VA hypobaric therapy of anxiety disorders neuroses and psychosomatic diseases. Patent RU (11) 2193382 (13) C2 (51) 7 A61G 10/02.
The method of rehabilitation after hypoxia, which causes dysfunction of the brain in models in laboratory animals, including the effects of hypobaric hypoxia in the chamber, characterized in that at 24 h after severe hypoxia model is placed in a pressure chamber and implement hypoxic postconditioning at atmospheric pressure 360 mm RT. Art., duration 2 h with an interval of 24 h for three days.
SUBSTANCE: modelling a myocardial infraction is followed in a rat is followed by intravenous introduction of the immune corrector tamerite 3 mg/kg. The preparation is injected daily for seven days. The histological results of the myocardial tissue presented after the first, third and seventh days show the sufficiency and efficacy of the therapy.
EFFECT: effective therapy of the acute myocardial infraction with high possibility of cure ensured by reduced manifestations of systemic inflammation response syndrome, faster granulation tissue formation and necrotic zone replacement by cicatricial tissue.
SUBSTANCE: hypoestrogen-induced endothelial dysfunction correction in white female Wistar rats, hypoestrogen-induced endothelial dysfunction is simulated by bilateral ovariectomy. The dysfunction correction is ensured by daily intragastric introduction of lozartan 6 mg/kg and daily introduction of mixed solutions of homoeopathic dilutions of polyclonal rabbit C12, C30, C200 antibodies to human endothelial nitrogen oxide synthase once a day added to drinking bowls.
EFFECT: method provides effective correction with reducing an endothelial dysfunction coefficient in females to the level observed in intact animals ensured by the effect on various developmental mechanisms of this dysfunction in hypoestrogenic conditions.
1 ex, 2 tbl
SUBSTANCE: reservatrol efficacy is studied in female Wistar rats with simulated osteoporosis by bilateral ovariectomy. For osteoporosis correction, reservatrol is administered in animal intraperitoneally for eight weeks after ovariectomy daily once a day in single dose 2 mg/kg. Osteoprotective action of reservatrol is estimated by a bone tissue microcirculation level and width of trabeculas of bone.
EFFECT: improved blood circulation and microarchitectonics of bone tissue in hypoestrogenic condition.
1 tbl, 1 ex
SUBSTANCE: efficacy is studied in female Wistar rats. Osteoporosis is simulated by bilateral ovariectomy. Osteoporosis is corrected by intraperitoneal introduction of reservatrol 2 mg/kg and intragastric introduction of enalapril 0.5 mg/kg daily once a day for eight weeks after ovariectomy. Osteoprotective action of reservatrol combined with enalapril is estimated by a bone tissue microcirculation level and width of trabeculas of bone.
EFFECT: improved blood circulation and microarchitectonics of bone tissue in hypoestrogen condition.
1 tbl, 2 ex
SUBSTANCE: delimitation of a high-grade glioma invasion is ensured by imaging of an astroglial bank surrounding the high-grade glioma. An immunogenic recombinant human GFAP is prepared and used to immunise a Balb/C mouse; spleen B-lymphocyte of this mouse are recovered and fused with myeloma cells of Sp 2/0-Ag14 mice; hybridomas are produced. Supernatants of the prepared hybridomas are tested by immunochemical techniques for the presence of anti-GFAP antibodies used to select a hybrid cell clone producing the anti-GFAP antibodies able to distinguish GFAP in vivo. The anti-GFAP antibodies are cleaned from the supernatant of the selected clone and covalently bound with liposomal nanocontainers containing a diagnostic mark. The antibodies of the selected hybrid cell clone is modified by g-amino groups of lysine residues and incubated with the stelths-liposome solution. The prepared nanosystem is introduced in a patient's vascular bed, and the astroglial bank is imaged by the arrangement of the diagnostic mark in cerebral tissues.
EFFECT: method allows preventing relapses of high-grade gliomas after their surgical management by choosing an optimal extent of a pending surgery ensured by delimitation of a tumour invasion by means of imaging of the astroglial bank.
6 cl, 4 dwg, 1 ex
SUBSTANCE: originally, epiphysial hip dysplasia is formed in 10-day-old golden hamsters with projection of the joint exposed to local infra-red pulse laser light daily for 20 days. Then, on the following day, simple hip dislocation of an experimental extremity of a hamster is performed by gradual extension of a capsular-ligamentous apparatus of the hip joint. Ventral and dorsal zones of a joint space are subject to mechanical vibrations by a border of a thin metal plate fixed in a stably fixed vibrator. The following parameters are used: joint capsule pressure 1 kg, vibration frequency 100 Hz, amplitude 0.3 mm for 15 minutes to the moment of femoral head exit from an acetabular cavity. Then, the femoral head is displaced manually behind an external surface of the cavity. In 2 days, the hamster is laid on its back, and the experimental hip is gradually taken aside from a vertical plane to a position of an apex of the head of the hip joint in a horizontal plane. The procedure is performed daily for at least 3 times for 7 days.
EFFECT: use of the given invention allows performing sparing simulation of the simple hip dislocation similar to a mechanism of hip dislocation pathogenesis in children that testifies to possibility of the use of this model for studying macro- and microscopic changes in the ends of the bones formed during growth.
5 dwg, 4 ex
SUBSTANCE: in order to develop methods of treating obstructive purulent cholangitis narrowing of general bile duct at 2/3 of lumen is carried out by application of cup. After that, emptying of gallbladder is realised with simultaneous introduction as microbial suspension - suspension of E.Coli strain 303 in dilution 0.95*10^5 CFU/ml on sterile solution of 0.9% sodium chloride, in volume 0.5 ml. Microbial suspension is introduced into lumen of general gall duct above the place of cup application. Said narrowing of general bile duct lumen is supported during entire experiment duration during 10-12 days.
EFFECT: method makes it possible to obtain adequate model of said pathology due to combined influence of factors, which lead to development of morphological and patho- physical malfunctions, most closely reflecting essence of obstructive purulent cholangitis.
SUBSTANCE: invention can be used both for studying mechanisms of development of paroxysmal states with progredient course (epilepsy) and for screening (selection) of novel medications, possessing potential anti-epileptic activity. For this purpose, rats are given fractional injections of convulsant 1.5-pentamethylentetrazole (pentylenetetrazole) with 15 minute interval in dose 10 mg/kg in volume 0.1 ml solution per 100 g of body weight until fist generalised convulsive seizure develops. After that calculated is threshold dose of convulsant reflecting base level of CNS excitability for each animal. When sessions are repeated, dynamics of change of initial dose of pentylenetetrazole is taken into account, interval between sessions of pentylenetetrazole titration can constitute from 24 and more than 168 hours.
EFFECT: method ensures high accuracy of evaluation of change of seizure development threshold due to the fact that expression of modelled progredient state can be changed at any stage of carrying out chronic research without application of special methods of examination.
1 tbl, 2 dwg
SUBSTANCE: in order to correct endothelial dysfunction white male rats of Wistar line have endothelial dysfunctions modelled by intro-peritoneum introduction of N-nitro-L-arginine methyl ether in dose 25 mg/kg daily, for seven days. Degree of dysfunction development is estimated by ratio of indices of endothelium-independent and endothelium-dependent vasodilation. Correction of endothelial dysfunction is carried out by intra-peritoneum introduction of medicine "Etoxydol" in dose 25 mg/kg one time per day, for 7 days in the morning.
EFFECT: correction of endothelial dysfunction by novel derivative of 3-oxypyridine in specially developed for this purpose dose and mode of medication introduction.
2 tbl, 1 ex
SUBSTANCE: to study etiology and pathogenesis of periodontitis, and in order to define tactics of treating periodontitis in case, when evident symptoms of periodontitis exacerbation are absent carried out is modelling of periodontitis on dogs by introduction in gum around neck of upper and lower incisors of ethanol in concentration 65-75% or corticosteroid medication in amount 0.3-0.4 ml per injection.
EFFECT: increase of model credibility of disease, similar in pathomorphological menifestations to periodontitis in humans, reduction of model obtaining terms.
SUBSTANCE: invention refers to medicine, particularly to hyperbaric physiology, and can be used for providing more efficient hyperbaric oxygenation in treating partial ankle ligamentous ruptures. That is ensured by combining hyperbaric oxygenation sessions with hot foot baths t=40-55°C with dry mustard added to water at 1 table spoon per 10 l of water.
EFFECT: method provides eliminated shin and foot vasospasm, reduced oedema within an injury, accelerates regeneration processes and reduces recovery time.
SUBSTANCE: invention concerns medicine, endocrinology, neurology. In women suffering climacteric syndrome, procedures of electrically induced sleep are carried out in one hour or one hour and a half after meals. Duration of a procedure is 30 minutes. In 30 minutes, it is followed by gravitational action in a direction from a head to lower extremities. Rotary speed is 32 rpm. The rotary speed is increased by 1 turn with each following session to 36 rpm. The maximum rotary speed is maintained to the end of the therapeutic course. A session involves muscular exercises of power 10 Wt gradually increased to 50 Wt to the end of the therapeutic course. Duration of a procedure is 10-12 minutes; the total therapeutic course is 10-15 days, 1 session a day.
EFFECT: invention allows providing minimum vestibular reactions and negative action on cerebral circulation with the greatest hypergravities at a level of small pelvic organs and lower extremities, improving metabolic processes, preventing venous hyperemia.
SUBSTANCE: compound modulus comprises a casing representing a rimmed oval cylinder, a buried end surface and a flange. An assembly fixture is mounted on the buried end surface and comprises extension clamps. The clamps accommodate a carrier plate flush mounted with the rim when the clamps are not extended. The modulus also comprises a control system in the casing accommodating a communication controller, a communication port attached to the communication controller and projecting from a first hole, and the communication port attached to the communication controller and accessible through a second hole. The first hole passes through the assembly fixture and the buried end surface, and the second one - through the flange. According to the other version, the compound modulus accommodates a casing, a processor built in the casing, a means for processor connection to an external control system, a means of casing connection to the external control system and a means of casing connection to the second compound modulus. The low-pressure therapy is performed by a system which comprises the control system accommodating the controller for therapy and the communication controller. The communication port is attached to the communication controller. A low-pressure source is attached to the controller for therapy. A main assembly is integrated in the flowing connection with the low-pressure source. A removable compound modulus is attached to the control system through the communication port.
EFFECT: use of the group of inventions shall enable enhancing the low-pressure therapy systems.
21 cl, 10 dwg
SUBSTANCE: invention relates to medicine, namely to dermatology and physiotherapy, and can be used for treatment of psoriasis vulgaris. Method of treating psoriasis vulgaris is realised by means of speleothgerapy at the background of traditional pathogenetic drug treatment by obtaining 16-18 day course in hospital ward, equipped with salt screens from natural syvinite with area not less than 2.6 m2 each per one bed, with ratio of salt surface area to total area of ward fencing surface 1:12.
EFFECT: increased efficiency of therapeutic treatment of psoriasis vulgaris.
SUBSTANCE: invention relates to rehabilitating medicine, namely to physiotherapy. Method includes basic therapy, speleoclimatotherapy, foamy bubble baths. Daily on empty stomach one hour before breakfast, six day running with break on the seventh day, treatment with individually selected homeopathic monopreparations is performed. Treatment in speleoclimatic chamber is carried out in first half of the day, for 60 minutes, daily. After the end of speleotherapy session patient gets 250 ml of foamy oxygen cocktail from licorice on mineral water from spring 104-P of Psekupskoye deposit. Foamy bubble baths with dense extract of licorice root are taken in the second half of the day. Baths are alternated with days of discontinuous nornobaric hypoxytherapy with course of 12 procedures.
EFFECT: method reduces drug load on organism, slows disease progressing, increases remission duration, increases tolerance to physical exercise, improves respiratory functions.
2 ex, 2 tbl
SUBSTANCE: invention relates to medicine and can be applied at necessary of defining of human's steadiness degree to hyperoxic hypoxia. For this purpose carried out is session of oxygenobarotherapy (OBT) with duration 60 minutes with partial oxygen pressure 0.25 MPa. Every 15 minutes during the session minute blood volume (MBV) is determined. If MBV increases during first 45 minutes of OBT session, person is considered non-resistant to hyperoxic hypoxia.
EFFECT: method makes it possible with to define of human resistance to said type of hypoxia with high accuracy.
SUBSTANCE: invention relates to medicine, namely, to neonatology, and can be applied in treatment of hyperbilirubinemia in newborn children. For this purpose carried out are sessions of hyperbaric oxygenation with purified moistened oxygen. During first session isopression is carried out at pressure 1.15 atm, at second session - 1.20 atm, and at third and following - 1.25 atm, with session duration 20-25 minutes. Treatment course consists of 5-7 sessions daily.
EFFECT: invention makes it possible to ensure impact on pathogenic mechanisms of said pathology development with simultaneous minimisation of complications.
SUBSTANCE: invention relates to field of medicine, namely - to physiotherapy. Method includes placing of patient into booth with placing head out of the latter. Booth is hermetised. After increasing temperature inside booth barometrical pressure is changed in impulse mode higher and lower than value of surrounding atmospheric pressure. Barometrical pressure is changed by displacement of, at least, one surface, limiting internal booth volume. At the end of procedure medications are additionally introduced into internal volume of booth. Device of physiotherapeutic impact for method realisation represents mini-sauna in form of booth with rigid walls and possibility of internal volume hermetisation in work position, with system of steam, aerosols feeding and thermoregulation. Internal surface of, at least, one wall, is made with possibility to change incidence angle and/or configuration. From external side, wall contacts with drive mechanism, which initiates listed changes.
EFFECT: method ensures efficient purification of skin pores, introduction of therapeutic substances through pores, contactless massaging of all body surface at the same time due to contactless impact, realised by means of physiotherapeutic device.
8 cl, 6 dwg
SUBSTANCE: invention refers to medical equipment, particularly to physiotherapeutic devices. An apparatus comprises a bedplate where there are a patient's head pillow and a heat chamber provided with a system for pressure control and regulation of a gas medium filling the heat chamber, front and back flanges whereto flexible sealing hoses made of an air-tight material are attached, and a sealing cuff for contacting with a patient's body mounted on a free end of the sealing hose attached to the front flange. In addition, the heat chamber is equipped with a cap provided to seal body parts being out of the sealing hoses; herewith the system for pressure control and regulation of a gas medium filling the heat chamber is equipped additionally with a heat chamber overpressure regulator.
EFFECT: invention allows to provide design universality with respect to enabling abdominal decompression and normal oxygen barotherapy.
4 cl, 3 dwg, 1 tbl
SUBSTANCE: invention relates to medical equipment and may be used in sports medicine and for therapy and prophylactics of vascular diseases of human extremities. Hypobaric device comprises local pressure chamber with unit of extremity sealing made of elastic material, device of pressure measurement, device of pressure reduction in pressure chamber, manifold of oxygen supply to pressure chamber and manifold of oxygen supply into mask arranged at the distance from face for inhalation of oxygen and air mixture. Sealing unit is arranged in the form of rubber sheet with hole in the centre with diametre of 35-105 mm and is connected to pressure chamber by connection element with the possibility of drawing into cavity of pressure chamber and adjacency to extremity in case of low pressure supply. Elastic material is a sheet of rubber with thickness of 1.5-3 mm.
EFFECT: invention provides for simplicity and reliability.
5 cl, 3 dwg
FIELD: medicine, obstetrics, gynecology.
SUBSTANCE: rehabilitation should be carried out in the third trimester of pregnancy based upon dispensary situated in ecologically safe region, as an enterosorbent one should apply "Carbopect" preparation per 0.5-0.6 g once daily for 3 wk; as an iodine preparation it is necessary to prescribe "Potassium iodide" 200 mcg once daily till the end of pregnancy period and during the whole period of lactation; as adaptogens one should additionally prescribe "Revit" per 2 lozenges thrice daily for 2 wk; vitamin-containing tea; one should, also, apply aerotherapy named "mountain air" per 15-40 min, 15 procedures, totally. The present innovation enables to improve anthropometric parameters in neonatals, decrease the percentage of complications in babies in early neonatal period, among them those that require antibioticotherapy.
EFFECT: higher efficiency of rehabilitation.